Thermo Scientific CellInsight CX5 HCS Platform. discover more uncover the possibilities
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1 Thermo Scientific CellInsight CX5 HCS Platform discover more uncover the possibilities
2 high content screening Driving your Productivity with Innovated Technology What is High Content Screening (HCS)? High Content Screening (HCS), also known as High Content Analysis (HCA), image cytometry, quantitative cell analysis or automated cell analysis, is an automated method that is used to identify substances that alter the phenotype of a cell in a desired manner. This technology is primarily used in biological research and drug discovery and combines fluorescent microscopy, automated cell calculations and phenotyping using image processing algorithms and informatics tools for the user to make decisions about a treatment. ASSAY FORMAT High Content Analysis Flow Cytometry Geneblazer Technology Luciferase SPA RIA ELISA Substrate Conversion Good Capability DETECTION MODE Multi Spectral Imaging and Screening Multi Laser PMT Ratio Fluoriometric Bioluminescence Radiometric Radiometric Colorimetric/Fluoriometric Colorimetric/Fluoriometric Radiometric Limited Capability Intensity Based Cell Based Poor Capability Intact Cells Multiplex Capability Individual Cell Measurements Subcellular Structure ** Adapted from: Keefer, S., S., and and Zock, Zock, J. In J. Approaching In High High Content Content Screening Screening and Analysis: and Analysis: Practical Practical Advice Advice for Users; for Users; Haney, S.A., Eds.: High Content Screening: Science Haney, S.A., Techniques Eds.: High and Content Applications. Screening: Wiley-Interscience, Science Techniques 2008, and pp. Applications Wiley-Interscience, 2008, Multicellular Structure Location How Does HCS Compare to Other Cell-Based Assays? Using a combination of established technologies, HCS can address both cellular level intensity and morphological measurements. With sufficient resolution for subcellular detection, automated detection and phenotyping can be achieved with intact, fixed or live cells. The table above describes some common cell-based assays used in laboratories. Visit our website at thermoscientific.com/highcontent
3 The Inventors and Leaders in HCA The Thermo Scientific Cellular Imaging and Analysis product line (formerly Cellomics) offers a full complement of hardware, analysis and control software, and informatics database tools to fully implement a high content program in your laboratory. With powerful software analysis and scalable informatics software, you can address hundreds of different biological phenotypes to discover more about a cell and its function. To order or request additional information, call USA Asia Europe +32 (0)
4 unmatched performance Reliably Scale your Throughput CellInsight CX5 HCS Platform Small in size but massive in its power, the Thermo Scientific CellInsight CX5 High Content Screening (HCS) Platform brings automated quantitative cell analysis to every cell biology or screening lab. Transform your cell-based research using the CellInsight CX5 HCS Platform by analyzing single cells in up to five fluorescent colors. With proprietary autofocus and integrated plate scanning intelligence methods, the CellInsight CX5 HCS Platform brings speed and accuracy to investigate cell populations and phenotypes without sacrificing sensitivity and resolution. Visit our website at thermoscientific.com/highcontent
5 IL10 IL2 KRT5 LCK LEP DLP SKD WOC 7DJS J5S8 KSU7 SUB3 SNWE PHF8 PIP PKC RHO SDHB AGP PC53 RISD 3NSD 4SD6 IL2 ZBTB 7AC3 CREBB CPH CHCP4 DHFR GAST HFE SRY BCL2 AL NPPB DLM RGL2B RGL2 MCM6 MYH7 MYOD1 GSLKD SD79S US3LS S72DF D3N4 SKD70 DLP 83J3 8SJ0 9D2E See More in Less Images The cutting-edge X1 camera is the most powerful HCS imaging tool to boost quantitative performance. See more of your biology in less images with the enlarged 2208 x 2208 pixel array. By ensuring reliability and reproducibility, the X1 camera s CCD technology affords you the confidence to trust your data. Brightfield Capabilities Designed to work with or without fluorescent labeling, the CellInsight CX5 HCS Platform provides transmitted light, allowing you to explore more cell biology without the restriction of fluorescent dyes. Scalable Cell Biology The CellInsight CX5 HCS Platform addresses the problem of scale with a continuum of solutions depending on the need, compatible with slides all the way through 1536-well microplates. The CellInsight CX5 HCS Platform connects directly to the Thermo Scientific Orbitor RS Microplate Mover to add up to 80-plate processing capacity. With included Application Programming Interface s (API), the CellInsight CX5 HCS Platform can be integrated into any third-party automation platform To order or request additional information, call USA Asia Europe +32 (0)
6 re-analyzing cell analysis The HCS Studio Advantage Image Analysis that Makes Sense The Thermo Scientific HCS Studio Cell Analysis Software offers simple workflows to help you develop and optimize an assay without functionality loss. With simple and straightforward task-oriented approaches to image algorithm development, new users are immediately productive. When a more complex assay is designed, HCS Studio Cell Analysis Software scales seamlessly, providing a host of algorithmic, visualization and online help tools that reduce the complexity and time investment of developing assays. From Optimized Assay Settings Users of HCS Studio Cell Analysis Software start with more than 30 pre-established assays that can be optimized to cellline and particular phenotype. Driven by intuitive icons, you can simply choose the assay and magnification of the optimized protocol, confirm settings, and begin the scan. We ve done the work, so you don t have to. M G2 S G1 Cell Cycle Assay Zebrafish Toxicity Assay Synaptogenesis Assay DNA Damage Assay Myotube Formation Assay Migration Assay Invasion Assay Cell Motility Assay Autophagy Assay Apoptosis Assay Build Your Own Assay Create your own assay from scratch using the flexible tools and design of HCS Studio Cell Analysis Software. With instant feedback you can control hundreds of options including: Background Correction Object Segmentation Spot/Granular Detection Region of Interests Sensivitity of Detection Projected Image Format Contrast of Images Phenotypic Gating Visit our website at thermoscientific.com/highcontent
7 Population Characterization Getting More From HCA Your assay deserves more. Whether using well-level measurements for total population responses or single-cell measurement for screening, HCS Studio Cell Analysis Software and its visualization tools allow you to investigate responses from the hundreds of measurements made. You can then combine single-cell phenotypic measurements and generate Events that can describe a subpopulation of cells using the multiplexed capability of HCA. The tremendous power of high content and Thermo Scientific High Content Tools allow you to quickly and accurately build phenotypic experiments in a single automated run. Individual Cell Level HCS Studio Cell Analysis Software is the most powerful tool in cell-based research because of its ability to report hundreds of measurements from each individual cell, monitoring even the most subtle changes that occur. Averaged Per Condition When thousands of cell responses are needed, then HCS Studio Cell Analysis Software simply delivers with treatment averages and relevant statistics that describe the population response. Cell-level Phenotyping By combining the power of cell analysis tools with the ability to create phenotypic events, HCS Studio Cell Analysis Software becomes more than just a high content tool, it becomes a cell phenotyping platform that transforms ordinary data into extraordinary results. To order or request additional information, call USA Asia Europe +32 (0)
8 thermoscientific.com/highcontent 2014 Thermo Fisher Scientific Inc. All rights reserved. Oracle is a trademark of Oracle. SQL Server is a trademark of Microsoft Corporation. All other trademarks are the property of Thermo Fisher Scientific Inc. and its subsidiaries. Specifications, terms and pricing are subject to change. Not all products are available in all countries. Please consult your local sales representative for details. USA info.cellomics@thermofisher.com Asia info.cellomics.asia@thermofisher.com Europe +32 (0) info.cellomics.eu@thermofisher.com C-BR_CX50414
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