Gerhard Schomburg. Gas Chromatography. A Practical Course. -«WTM-JY// ^ Weinheim New York. VCH XJ Basel Cambridge
|
|
- Owen Gaines
- 8 years ago
- Views:
Transcription
1 Gerhard Schomburg Gas Chromatography A Practical Course -«WTM-JY// ^ Weinheim New York VCH XJ Basel Cambridge
2 Contents 1 The Role of Gas Chromatography Among Other Methods of Instrumental Analysis 1 2 Design and Functions of Instrumentation for Gas Chromatography 5 3 The Basic Theory of Chromatographic Separations 9 4 The Gas Chromatogram The Time Coordinate of the Gas Chromatogram Retention Time or Volume Relationships between Retention Time, Capacity Ratio and Partition Coefficient Peak Width, Theoretical Plate Number, Separation Efficiency Resolution Relationships between Resolution, Efficiency and Capacity Ratio The Intensity Coordinate of the Gaschromatogram 26 References for Sections Journals and Periodicals in which Publications on Gas Chromatography Topics may Appear 29 5 Elements and Parameters of Gas Chromatographic > irf -"&. Instrumentation GC Instruments and their Optimization Separation Columns Packed Columns Capillary Columns Connection Techniques for Capillary Columns Couplings of Capillary Columns Optimization of Column Parameters Column Temperature Carrier Gas Flow Column Ovens Sample Introduction Techniques Sample Volume Split Injection: Sample Introduction with External Vaporization with Splitting of the Carrier Gas Flow 53
3 VIII Contents Splitless Injection of Dilute Samples: Sample Introduction with External Vaporization without Splitting On-Column Sample Introduction Temperature-Programmed Vaporization: Cold Sample Introduction and Temperature-Programmed External Vaporization Introduction of Gaseous Samples Head Space Sample Introduction Gas Chromatographic Detectors Detectors with Concentration-Dependent Signal Intensity Detectors with Mass Flow-Dependent Signal Intensity General Properties and Parameters of GC Detectors GC Instruments for Practical Analyses: Systems and Functions Packed Columns in Combination with TCD and/or FID, Split and Splitless Sampling Combinations of Capillary Columns with FID, N, P-FID, ECD, Multichannel Detection and Various Sampling Devices such as Split, Splitless and On-Column Coupling of GC Systems to Mass or FTIR Spectrometers GC-MS Combination GC-FTIR Combination 80 References 82 6 Methods of Qualitative and Quantitative GC Analysis of Mixtures Qualitative Analysis Sample Types, Sample Pretreatment, Chromatographic Volatility of Components Considerations before the Execution of a GC Separation Standard GC Instrumentation for Use with Capillary Columns Test Measurements and Optimization of the GC System before the Actual Analysis Qualitative GC Analysis of Samples of Unknown Composition Identification of the Separated Species Identification from Absolute and Relative Retention Data The Retention Index System The Kovats Retention Index Types of Intermolecular Interaction between Solute and Stationary Phase Retention and Chemical Structure of Solutes Comparison of Retention Indices of Two Compounds of Different Structure Obtained with the same Stationary Phase Comparison of Retention Indices of a Single Compound Measured with Two Stationary Phases of Different Polarity Temperature Dependence of Retention Indices, Quantitative GC Analysis Criteria of Good Quantitative Analyses for the Analytical Practice Quantitative Evaluation of Chromatograms via Peak Heights or Areas. 109
4 Contents IX Determination, Application and Prediction of Response Factors for Use in Quantitative Analysis via Peak Areas Ill Response Correction Factors for the TCD and the FID Determination of Response Factors Response Factors for TCD and FID, Influence of Chemical Structure Incremental Prediction of FID Response Factors Evaluation of the Concentrations of Components in the Sample from Peak Areas Absolute Calibration: Peak Areas versus Sample Volume The Internal Normalization Method of Chromatogram Evaluation via Peak Areas The Internal Standard Method of Chromatogram Evaluation via Peak Areas /. 120 References Special Methods, Auxiliary Techniques and Applications of Gas Chromatography Analysis of Samples of Complex Mixtures Separation of Volatile and/or Non-polar Samples. Gas Analysis by Gas Chromatography Separation of Compounds of Low Volatility; High Temperature GC Separation of Highly Polar Compounds; Derivatization Separation and Detection of Trace Components by Specific Detection Determination of Trace components of Known Chemical Structure; Trace Analysis for Target Compounds Specific Detection of Trace Components Automation of GC Analyses; Process Control GC Automated Execution of Sample Introduction, Separation and Detection Handling of GC Data by Computers Coupled GC Systems, Multi-dimensional GC Separations Analytical Objectives of Multi-Dimensional Separations Coupling of Two Capillary Columns with Different Stationary Phase Contents Coupling of Two Capillary Columns with Stationary Phases of Different Polarities Coupling of Two Capillary Columns in a Double Oven Instrument Using an ECD as Monitor Detector and Parallel Detection with FID and ECD for the Second (Main) Separation. Multi-dimensional GC of Chlorinated Aromatic Hydrocarbons Coupling of a Packed to Capillary Column Coupling of Chemical Reactors to GC Columns. Reaction Gas Chromatography Pre-column Reaction Gas Chromatography: On line Hydrogenation, PyrolysisGC 146
5 X Contents On-line Hydrogenation of Unsaturated Species Pyrolysis Gas Chromatography Post-Column Reaction Gas Chromatography Trace Analysis for CO and CO 2 by Post-column Hydrogenation On-Line Hydrogenation of Unsaturated Species After Elution from the Separation Column and Before Entering the Ion Source of the Mass Spectrometer Specific Detection of Oxygenated Hydrocarbons 151 References Sources of Analytical Error in Gas Chromatographic Systems: Elucidation and Elimination Recognition of Instrumental Errors in the Chromatographic System from the Analog Chromatogram Distortion of Peak Shapes Thermodynamic Reasons for Bad Peak Symmetry Overloading of a Non-polar Methylpolysiloxane Capillary Column by the Highly Polar Free Fatty Acids Instrumental Sources of Bad Peak Symmetry Influence of Flow Geometry Influence of Inadequate Execution of Sample Introduction Unsymmetrical and Overlapped Peak Shapes Originating from Thermal or Catalytic Conversion of Labile Sample Components Appearance of Too Broad Peaks in the Chromatogram The Peaks in Isothermal Chromatograms are Broad with Generally Bad Resolution Individual Peaks in the Isothermal Gas Chromatogram are Broader than the Neighbouring Peaks with Longer Retentions Appearance of Too Narrow Peak Profiles in the Entire Chromatogram Causes of More Complicated Peak Shapes Disturbances to the Baseline of the Chromatogram Elimination of Sources of Deteriorated Chromatogram Performance Sources of Error in Retention Data Measurements Elimination of Negative Effects on Retention Data Measurements Sources of Error in Quantitative GC Analysis Problems with the Evaluation of the Correct Baseline for Quantitation of Insufficiently Resolved Chromatograms of Complex Preferably Isomeric Mixtures 170 References Analytical Supercritical Fluid Chromatography Principles and Methods for SFC Instrumentation for SFC Columns and Column Ovens Pumping Systems for Mobile Phase Delivery 178
6 Contents XI Sample Introduction Devices Restrictors Detectors Typical Applications of SFC 181 References The Coupling of LC and GC Pretreatment of Complex Samples by Liquid Chromatography with Direct Transfer of Eluate Cuts into Capillary GC Systems 189 References Gas Chromatographic Applications Using Different Methods and Techniques Characteristics and Criteria of Good GC Separations with Regard to Qualitative and Quantitative Objectives of Analysis Isothermal Separation of a Series of Homologous n-alkanes Temperature-Programmed Separation of a Series of. Homologous n-alkanes Temperature Programmed Separation of Complex Hydrocarbon Mixtures Temperature-Programmed Separation of a C 10 -C 25 Petroleum Fraction Temperature-Programmed Separation of a Petroleum Fraction Using a Narrow-Bore Capillary Column Separation of the Phytane Diastereomers (C 20 tetraisoprenoid) by Optimization of Efficiency, Temperature and Polarity Using a PEG (CW 20M) Column Saturated and Unsaturated Volatile CJ-CJ Hydrocarbons C r C 5 Hydrocarbons Separated with a Column Packed with a Porous Polydivinyl Polymer CJ-CJ Hydrocarbons Separated with GLC Capillary Columns with a Thick Film Stationary Phase Coating GSC Capillary Columns Coated with Alumina Test Sample of Components Applied as Propellents for Spray Cans Low Molecular Weight Halogenated Hydrocarbons with Electron Capture Detection, ECD Halocarbon Compounds Contained in Tap Water Using an Apolar Thick-Film Capillary Column and Electron Capture Detection Polychlorinated Biphenyls (PCB's) Using a Narrow Bore Capillary Column and Electron Capture Detection Highly Polar Compounds Using a Polar PEG Capillary Column and Thermal Conductivity Detection Aqueous Solution of Apple Flavor Compounds Using a PEG Capillary Column with Thermal Conductivity and Flame Ionization Detection 218
7 XII Contents Determination of Aromatic Hydrocarbons and Oxygenates such as Methanol, tert.-butanol and Isobutanol in Gasoline Multi-dimensional Separation of Aromatic Hydrocarbons and Oxygenates such as Aliphatic Alcohols and Methyl tert.-butyl Ether Solvent Mixture Containing Hydrocarbons, Ethylene Glycol and Isomeric Phenols Separated Using a Polyethylene Glycol Column Test Mixtures for the Characterization of the Surface Adsorptivity of Capillary Columns Grob-Test for Efficiency, Polarity and Adsorptivity of a Non-polar Methylpolysiloxane OV-1 Column Grob-Test of a Methylpolysiloxane OV-1 Column with Incompletely Dealkalized Support Surface Grob-Test of a Polar Polyethylene Glycol (CW 20 M) Column Hindelang Round-Robin Capillary Column Test Mixture High-Speed Separation of Alkylbenzenes with a Capillary Column of Small Inner Diameter Polyaromatic Hydrocarbons from Coal Tar, Separation with a Thin Film Methylpolysiloxane OV-1 Column Polyaromatic and Heterocyclic Hydrocarbons, Simultaneous Detection with FID and NFID Saturated and Unsaturated Hydrocarbons Originating from Date Fruits, Separated with and without On-line Hydrogenation Saturated and Unsaturated Fatty Acid Methyl Esters Obtained from the Triglycerides of Fish Oil Triglycerides of Fish Oil Separated with a Non-selective Methylpolysiloxane Column Fatty Acid Methyl Esters (FAME) Obtained from Fish Oil by Transesterification Separated with an Apiezon M Glass Capillary Column with and without On-line Hydrogenation Unsaturated C 18 -FAMEs Obtained from Lipids of Pimpinella anisium Cell Cultures and from Triglyerides of Fish- and Sunflower Oil Compared with FAMEs in a Test Mixture, Separated with a 120 msilarlocp Glass Capillary Column Triglyerides of Palm Oil Separated with a Methylpolysiloxane (OV-1) and a Polar Methylphenylpolysiloxane (OV-17) Column With a Non-Polar OV-1 Column, the Elution Sequence of the Different Triglycerides Depends on: With the Polar, Phenyl-Substituted OV-17 Methylpolysiloxane Column, the Elution Sequence is Different and Depends on: Test Mixture of Morphine Drugs Trace Concentration of Polychlorinated Biphenyls (PCBs) in Used Mineral Oils by Simultaneous Detection with FID and ECD
8 Contents XIII Analysis of Kirsch Test Mixture Containing C 2 -C 5 Diols Phenols, Cresols and Xylenols Chlorophenols with Flame Ionization- and Electron-Capture Detection FID ECD Phenols Derivatized by Silylation, Detection with FID and ECD Polychlorinated Dibenzo-p-dioxins and Dibenzofurans Polychlorinated Dibenzo-p-dioxins and Dibenzofurans, Detected by ECD Tetrachlorinated Dibenzo-p-dioxins Detected by GC-MS-SIM Recorded at m/z Multi-Dimensional GC-analysis for 2, 3, 7, 8-TCDD Aroma Extracts from Beer and Wine Separated with an Apolar Methylpolysiloxane OV-1-Column Acetylated Polyalcohols, Alditols and Some Amino Derivatives Chiral Stationary GC Phases for the Separation of Enantiomers Enantiomer Separation of Underivatized Prominal and Evipan N-Pentafluoropropanoyl Derivatives of the Isopropyl Esters of DL-Amino Acids. Separation of a Test Mixture of 17 DL-Amino Acids and a Total Hydrolysate of the Peptide Antibiotic Herbicolin A Multi-Dimensional Separation of the Isopropylurethanes of the Four Diastereomeric Menthols Trifluoroacetylated a- and p-pyranosidic Hexoses: Galactose, Mannose and Glucose Isopropylurethanes of Eight Enantiomeric Pairs of 2-Aminoalkanes Enantiomeric y-lactones Separated with Hexakis (3-O-butyryl-2, 6- di-o-pentyl) -oc-cyclodextrin Enantiomer Separation of 3-Methyl-1 -hexene, 2-Bromobutane and 3-Methyl-cyclohexene with Perpentyl-p-cyclodextrin Enantiomer Separation of Limonene, Camphene and a-pinene Using Heptakis (3-O-methyl-2,6-di-O-pentyl)-(3-cyclodextrin Important Equations and Terminology of Gas Chromatography Equations Total or Gross Retention Time, t^ Net Retention Time, t s and Dead Time, t m ' Capacity Ratio, k' Capacity Ratio and Partition Coefficient, K, Phase Ratio, ( Absolute Retention Data, Corrected Net Retention Volumes Relative Retention Data Relative Volatility Selectivity Coefficient Retention Index Kovats Retention Index, I of a Single Solute x 293
9 XIV Contents Difference, AI, of Retention Indices of the Same Solute Obtained with Different Stationary Phases Linear Retention Index Theoretical Plate Number N Van Deemter-Golay Equation Resolution of Chromatographic Separations Separation Number (Trennzahl), TZ Glossary of Terms Used in the Theory and Practice of Gas Chromatography List of Symbols and Acronyms Index 313
Lecture Chromo-3: Gas Chromatography. CHEM 5181 Fall 2004 Mass Spectrometry & Chromatography. Jessica Gilman and Prof. Jose-Luis Jimenez CU-Boulder
Lecture Chromo-3: Gas Chromatography CHEM 5181 Fall 2004 Mass Spectrometry & Chromatography Jessica Gilman and Prof. Jose-Luis Jimenez CU-Boulder Outline Introduction Instrument overview Carrier gas Sample
More informationGas Chromatography. Gas Chromatograph
Gas Chromatography Gas Chromatograph Chromatography: Separate analytes in a mixture with a resolution 1.5 in the shortest amount of time and detection of separated components. 1 = m.p. 6 2 4 5 3 Gas supplies
More informationGC METHODS FOR QUANTITATIVE DETERMINATION OF BENZENE IN GASOLINE
ACTA CHROMATOGRAPHICA, NO. 13, 2003 GC METHODS FOR QUANTITATIVE DETERMINATION OF BENZENE IN GASOLINE A. Pavlova and R. Ivanova Refining and Petrochemistry Institute, Analytical Department, Lukoil-Neftochim-Bourgas
More information1.1 This test method covers the qualitative and quantitative determination of the content of benzene and toluene in hydrocarbon wax.
Standard Method for Analysis of Benzene and Toluene Content in Hydrocarbon Waxes by Headspace Gas Chromatography EWF METHOD 002/03 (Version 1 Reviewed 2015) 1 Scope 1.1 This test method covers the qualitative
More informationBTU ANALYSIS USING A GAS CHROMATOGRAPH
BTU ANALYSIS USING A GAS CHROMATOGRAPH By Joseph E. Landes Southern Petroleum Laboratories, Inc. Technical Director Hydrocarbon and Field Services 8820 Interchange Drive Houston, Texas 77054 Introduction
More informationTheory and Instrumentation of GC Introduction
Theory and Instrumentation of GC Introduction i Wherever you see this symbol, it is important to access the on-line course as there is interactive material that cannot be fully shown in this reference
More informationAn Advanced Base Deactivated Capillary Column for analysis of Volatile amines Ammonia and Alcohols.
An Advanced Base Deactivated Capillary Column for analysis of Volatile amines Ammonia and Alcohols. Jaap de Zeeuw, Ron Stricek and Gary Stidsen Restek Corp Bellefonte, USA To analyze basic compounds at
More informationChemistry 321, Experiment 8: Quantitation of caffeine from a beverage using gas chromatography
Chemistry 321, Experiment 8: Quantitation of caffeine from a beverage using gas chromatography INTRODUCTION The analysis of soft drinks for caffeine was able to be performed using UV-Vis. The complex sample
More informationFractional Distillation and Gas Chromatography
Fractional Distillation and Gas Chromatography Background Distillation The previous lab used distillation to separate a mixture of hexane and toluene based on a difference in boiling points. Hexane boils
More informationGas Chromatography. Let s begin with an example problem: SPME head space analysis of pesticides in tea and follow-up analysis by high speed GC.
Gas Chromatography Let s begin with an example problem: SPME head space analysis of pesticides in tea and follow-up analysis by high speed GC. Samples in 10mL sealed glass vials were placed in the MPS-2
More informationFerda ORHUN Mineral Research and Exploration Institute of Turkey
GAS CHROMATOGRAPHY, EXPERIMENTAL STUDIES ON THE FLOW RATE EFFECTS OF CARRIER GAS AND APPLICATION OF THE METHOD TO HYDROCARBON ANALYSIS OF SOME NATURAL GASES IN TURKEY Ferda ORHUN Mineral Research and Exploration
More informationExpectations for GC-MS Lab
Expectations for GC-MS Lab Since this is the first year for GC-MS to be used in Dr. Lamp s CHEM 322, the lab experiment is somewhat unstructured. As you move through the two weeks, I expect that you will
More informationSPE and HPLC. Dr Iva Chianella Lecturer in Analytical Chemistry Cranfield Health +44 (0) 1234 758322. i.chianella.1998@cranfield.ac.
SPE and HPLC Dr Iva Chianella Lecturer in Analytical Chemistry Cranfield Health +44 (0) 1234 758322 i.chianella.1998@cranfield.ac.uk Solid-Phase Extraction- SPE Simple, fast and efficient sample preparation
More informationGet the Right GC for Your Industry
Get the Right GC for Your Industry Shimadzu was founded on the basis of "Technology for Science" and we continue to take the initiative in the analytical technology industry. From our absorption spectrophotometers
More informationSplit / Splitless Injection for Capillary GC
Split / Splitless Injection for Capillary GC Speakers John V Hinshaw CHROMacademy GC Dean GC Connections editor Moderator Tony Taylor CHROMacademy Trainer Technical Director, Crawford Scientific Dave Walsh
More informationTABLE OF CONTENTS CHAPTER TITLE PAGE DECLARATION ACKNOWLEDGEMENT ABSTRACT LIST OF TABLES LIST OF FIGURES LIST OF SYMBOLS LIST OF APPENDICES
vii TABLE OF CONTENTS CHAPTER TITLE PAGE DECLARATION ii ACKNOWLEDGEMENT iv ABSTRACT v TABLE OF CONTENTS vii LIST OF TABLES xii LIST OF FIGURES xiv LIST OF SYMBOLS xvii LIST OF APPENDICES xviii 1 INTRODUCTION
More informationSeparation of Amino Acids by Paper Chromatography
Separation of Amino Acids by Paper Chromatography Chromatography is a common technique for separating chemical substances. The prefix chroma, which suggests color, comes from the fact that some of the
More informationAnalyzing Small Molecules by EI and GC-MS. July 2014
Analyzing Small Molecules by EI and GC-MS July 2014 Samples Appropriate for GC-MS Volatile and semi-volatile organic compounds Rule of thumb,
More informationUnit Vocabulary: o Organic Acid o Alcohol. o Ester o Ether. o Amine o Aldehyde
Unit Vocabulary: Addition rxn Esterification Polymer Alcohol Ether Polymerization Aldehyde Fermentation Primary Alkane Functional group Saponification Alkene Halide (halocarbon) Saturated hydrocarbon Alkyne
More informationIII Analytical Methods
III Analytical Methods III.1 Outline of chemical analysis in the environment III.1.1 Analytical order of chemicals in the environmental samples In order to be able to determine very small amounts (very
More informationDiffusion and Fluid Flow
Diffusion and Fluid Flow What determines the diffusion coefficient? What determines fluid flow? 1. Diffusion: Diffusion refers to the transport of substance against a concentration gradient. ΔS>0 Mass
More informationBackground Information
1 Gas Chromatography/Mass Spectroscopy (GC/MS/MS) Background Information Instructions for the Operation of the Varian CP-3800 Gas Chromatograph/ Varian Saturn 2200 GC/MS/MS See the Cary Eclipse Software
More information23 The Thermal Conductivity Detector
23 The Thermal Conductivity Detector General Information TCD pneumatics Conditions that prevent the detector from operating Filament passivation Carrier, reference, and makeup gas Negative polarity Analyzing
More informationPLOT Columns. Top: Bob Langford, GC Column Manufacturing Technician Bottom: Stephanie Sunner, Customer Service Representative
PLOT Columns Top: Bob Langford, GC Column Manufacturing Technician Bottom: Stephanie Sunner, Customer Service Representative PLOT Column Selection 93 Features & Benefits of Restek PLOT Columns Features
More informationAMD Analysis & Technology AG
AMD Analysis & Technology AG Application Note 120419 Author: Karl-Heinz Maurer APCI-MS Trace Analysis of volatile organic compounds in ambient air A) Introduction Trace analysis of volatile organic compounds
More informationAromatic plants: from extraction to analysis
Aromatic plants: from extraction to analysis Thierry TALOU Agro-industrial Chemistry Laboratory National Polytechnic Institute of Toulouse ENSIACET 4 allée Emile Monso 31432 Toulouse Olfactory system Olfactory
More informationChemical Analysis. Natural Gas Analyzer. A Family of optimized GC Solutions. Gas Chromatography. think forward
Chemical Analysis Natural Gas Analyzer A Family of optimized GC Solutions think forward Gas Chromatography Bruker Natural Gas Analyzers Natural gas is bought and sold as a bulk commodity with price based
More informationEXAMINATION IN COURSE KJ 2053 CHROMATOGRAPHY
NTNU Norwegian University of Science and Technology Faculty of Natural Sciences and Technology Department of chemistry Page 1 (of 13) Inquiries concerning the contents of the test to : Assoc. professor
More informationAddressing the World Helium Shortage For Gas Chromatography
Addressing the World Helium Shortage For Gas Chromatography Helium Conservation and Converting GC Methods to Nitrogen and Hydrogen Carrier Gas James D. McCurry, Ph.D. Senior Scientist Agilent Technologies
More informationBringing Innovations to Standards AC Analytical Controls by PAC All In One Biodiesel Analyzer
Bringing Innovations to Standards AC Analytical Controls by PAC All In One Biodiesel Analyzer www.paclp.com Background Biodiesel offers an alternative to fossil diesel fuel & contributes to the reduction
More informationPesticide Analysis by Mass Spectrometry
Pesticide Analysis by Mass Spectrometry Purpose: The purpose of this assignment is to introduce concepts of mass spectrometry (MS) as they pertain to the qualitative and quantitative analysis of organochlorine
More informationOverview. Triple quadrupole (MS/MS) systems provide in comparison to single quadrupole (MS) systems: Introduction
Advantages of Using Triple Quadrupole over Single Quadrupole Mass Spectrometry to Quantify and Identify the Presence of Pesticides in Water and Soil Samples André Schreiber AB SCIEX Concord, Ontario (Canada)
More informationShimadzu Simulated Distillation Gas Chromatograph System C184-E030
Shimadzu Simulated Distillation Gas Chromatograph System C184-E030 Meets All Simulated Distillation Gas Chromatograph Standards - Provides Highly Accurate Analysis Results with Excellent Reproducibility-
More informationChapter 5 Classification of Organic Compounds by Solubility
Chapter 5 Classification of Organic Compounds by Solubility Deductions based upon interpretation of simple solubility tests can be extremely useful in organic structure determination. Both solubility and
More informationMass Spectrometry. Fundamental GC-MS. GC Considerations
Mass Spectrometry Fundamental GC-MS GC Considerations i Wherever you see this symbol, it is important to access the on-line course as there is interactive material that cannot be fully shown in this reference
More informationUHPLC/MS: An Efficient Tool for Determination of Illicit Drugs
Application Note: 439 UHPLC/MS: An Efficient Tool for Determination of Illicit Drugs Guifeng Jiang, Thermo Fisher Scientific, San Jose, CA, USA Key Words Accela UHPLC System MSQ Plus MS Detector Drugs
More informationThe Characterization of Perfume Fragrances Using GC/MS, Headspace Trap and Olfactory Port
APPLICATION NOTE Gas Chromatography/ Mass Spectrometry Authors: Andrew Tipler Sheila Eletto PerkinElmer, Inc. Shelton, CT The Characterization of Perfume Fragrances Using GC/MS, Headspace Trap and Olfactory
More informationA new concept for isotope ratio monitoring LC/MS. A Wide Range of Applications
A new concept for isotope ratio monitoring LC/MS A Wide Range of Applications Andreas Hilkert, Dieter Juchelka, Michael Krummen Thermo Electron (Bremen) GmbH Overview Introduction in Isotope Ratio Monitoring-LC/MS
More informationTechnical Report. Automatic Identification and Semi-quantitative Analysis of Psychotropic Drugs in Serum Using GC/MS Forensic Toxicological Database
C146-E175A Technical Report Automatic Identification and Semi-quantitative Analysis of Psychotropic Drugs in Serum Using GC/MS Forensic Toxicological Database Hitoshi Tsuchihashi 1 Abstract: A sample consisting
More informationProcess Instrumentation
Process Instrumentation Analysis by Gas Chromatography Engineered Solutions, Guaranteed Results. WASSON - ECE INSTRUMENTATION Process Gas Chromatography Features of Process Chromatographs Agilent 7890A
More informationAN INTRODUCTION TO HEADSPACE SAMPLING IN GAS CHROMATOGRAPHY FUNDAMENTALS AND THEORY. Andrew Tipler
AN INTRODUCTION TO HEADSPACE SAMPLING IN GAS CHROMATOGRAPHY FUNDAMENTALS AND THEORY Andrew Tipler Chromatography Research and Technology Manager PerkinElmer, Inc. Table of Contents Introduction 3 Fundamental
More informationGas Separations: A Comparison of GasPro and Aluminum Oxide PLOT Columns for the Separation of Highly Volatile Compounds
Gas Separations: Comparison of GasPro and luminum Oxide PLOT Columns for the Separation of Highly Volatile Compounds Daniel W. rmstrong* Department of Chemistry, University of Missouri-Rolla, Rolla, MO
More informationCHROMOCTANE O N LINE ANALYZER
GECIL Process GT instruments CHROMOCTANE O N LINE ANALYZER ASTM D6733 DHA and RON and MON on line GC for Reformer unit Automatic Chromatographic system for analysis of naphta feeds and reforming gasolines
More informationPCBs DETERMINATION IN TRANSFORMER OIL BY SPE AND GAS CHROMATOGRAPHY ANALYSIS
PCBs DETERMINATION IN TRANSFORMER OIL BY SPE AND GAS CHROMATOGRAPHY ANALYSIS Bogdevich O.P. *, Cadocinicov O.P. * Institute of Geophysics and Geology, Laboratory of Geochemistry GEOLAB Academiei str. 3,
More information6. ANALYTICAL METHODS
45 6.1 BIOLOGICAL MATERIALS No methods were located that are routinely used for the detection of BCEE in biological materials. Norpoth et al. (1986) reported a method for measuring thiodiglycolic acid
More informationAnalysis of Liquid Samples on the Agilent GC-MS
Analysis of Liquid Samples on the Agilent GC-MS I. Sample Preparation A. Solvent selection. 1. Boiling point. Low boiling solvents (i.e. b.p. < 30 o C) may be problematic. High boiling solvents (b.p. >
More informationCRYOCOOLER CONTAMINATION STUDY. S.W.K. Yuan, D.T.Kuo, and A.S. Loc. BEI Technologies, Cryocooler Group Sylmar, CA 91342 ABSTRACT
CRYOCOOLER CONTAMINATION STUDY S.W.K. Yuan, D.T.Kuo, and A.S. Loc EI Technologies, Cryocooler Group Sylmar, CA 91342 ASTRACT Eliminating or reduction of contamination is essential for the success of cryocoolers
More informationCopyright 2010 Pearson Education, Inc. Chapter Fourteen 1
An alcohol has an OH bonded to an alkyl group; a phenol has an OH bonded directly to an aromatic ring; and an ether has an O bonded to two organic groups. Chapter Fourteen 1 Ethyl alcohol, dimethyl ether,
More informationAlignment and Preprocessing for Data Analysis
Alignment and Preprocessing for Data Analysis Preprocessing tools for chromatography Basics of alignment GC FID (D) data and issues PCA F Ratios GC MS (D) data and issues PCA F Ratios PARAFAC Piecewise
More informationHow To Use Gc-Ms
The CHROMacademy Essential Guide Understanding GC-MS Analysis Part 1 Speakers John Hinshaw GC Dept. Dean CHROMacademy Tony Taylor Technical Director Crawford Scientific Moderator M ( g ) e M ( g ) 2e Peter
More informationSelectivity and Detectability Optimizations in HPLC
Selectivity and Detectability Optimizations in HPLC SATINDER AHUJA Development Department Pharmaceuticals Division CIBA-GEIGY Corporation Suflern, New York WILEY A WILEY-INTERSCIENCE PUBLICATION JOHN WILEY
More informationGas Chromatography Liner Selection Guide
Gas Chromatography Liner Selection Guide Peter Morgan, Thermo Fisher Scientific, Runcorn, Cheshire, UK Technical Note 20551 Key Words Liner, focus Abstract The liner serves an important function in allowing
More informationDetermination of polyols derived from sugars and residual sugars found in dry wines by means of gas chromatography (Resolution Oeno 9/2006)
Method OIV-MA-AS311-06 Type IV method Determination of polyols derived from sugars and residual sugars found in dry wines by means of gas chromatography (Resolution Oeno 9/2006) 1. Scope Simultaneous determination
More informationQuantitative Evaluation of Perfume Fleuressence Samples using the znose
Quantitative Evaluation of Perfume Fleuressence Samples using the znose Edward J. Staples, Electronic Sensor Technology Electronic Noses Conventional electronic noses (enoses) produce a recognizable response
More informationSolid Phase Extraction Products PAGE: 1. Introduction of Solid Phase Extraction (SPE) Why Choose Nano-Micro Tech SPE
Solid Phase Extraction Products PAGE: 1 Introduction of Solid Phase Extraction (SPE) SPE has been used increasingly in chemical analysis and purification broadly, and become the most popular technology
More informationADVANCEMENTS IN MICRO GAS CHROMATOGRAPHY (GC)
ADVANCEMENTS IN MICRO GAS CHROMATOGRAPHY (GC) 1 Fast Analysis of C1 to C8 hydrocarbons for mud logging applications within 2 minutes using a temperature programmable Micro GC Fusion NEN GAS2015 Debbie
More informationAnalysis of the Vitamin B Complex in Infant Formula Samples by LC-MS/MS
Analysis of the Vitamin B Complex in Infant Formula Samples by LC-MS/MS Stephen Lock 1 and Matthew Noestheden 2 1 AB SCIEX Warrington, Cheshire (UK), 2 AB SCIEX Concord, Ontario (Canada) Overview A rapid,
More informationContents. Contributors. 1 Quality control and regulation 1 C.J. MOORES
Contents Contributors Preface xiii xiv 1 Quality control and regulation 1 C.J. MOORES 1.1 Introduction 1 1.2 The quality of medicines 2 1.2.1 The meaning of quality 2 1.2.2 Medicines are special 3 1.2.3
More informationIdentification of Arson Accelerants by Gas Chromatography
Identification of Arson Accelerants by Gas Chromatography Purpose Arson is an insidious crime that annually claims the lives of hundreds of Americans and costs billions of dollars. 1 Scientists and criminal
More informationGuide to Reverse Phase SpinColumns Chromatography for Sample Prep
Guide to Reverse Phase SpinColumns Chromatography for Sample Prep www.harvardapparatus.com Contents Introduction...2-3 Modes of Separation...4-6 Spin Column Efficiency...7-8 Fast Protein Analysis...9 Specifications...10
More informationCarbohydrates, proteins and lipids
Carbohydrates, proteins and lipids Chapter 3 MACROMOLECULES Macromolecules: polymers with molecular weights >1,000 Functional groups THE FOUR MACROMOLECULES IN LIFE Molecules in living organisms: proteins,
More informationTHERMAL DESORPTION. Introduction and Principles. Focusing on Volatiles
THERMAL DESORPTION Introduction and Principles Thermal Desorption Thermal desorption is a simple extension of the technique of gas chromatography (GC) It involves the use of heat and a flow of inert gas
More informationC184-E014D GC-2014. Shimadzu Gas Chromatograph System
C184-E014D GC-2014 Shimadzu Gas Chromatograph System Big Performance & Small Space GC-2014 GC-2014 Shimadzu Gas Chromatograph System High Performance and Expandability Merged at a higher level High Performance
More informationStrategies for Developing Optimal Synchronous SIM-Scan Acquisition Methods AutoSIM/Scan Setup and Rapid SIM. Technical Overview.
Strategies for Developing Optimal Synchronous SIM-Scan Acquisition Methods AutoSIM/Scan Setup and Rapid SIM Technical Overview Introduction The 5975A and B series mass selective detectors (MSDs) provide
More informationOn-line Dissolved Gas Analysis
On-line Dissolved Gas Analysis accord. to IEC 567/ASTM 3612 Online-Monitoring of transformers Automatic on-line analysis of 11 gases The analysis is done in 2 steps with high selectivity and accuracy fully
More informationAnalysis of Organophosphorus Pesticides in Milk Using SPME and GC-MS/MS. No. GCMS-1603. No. SSI-GCMS-1603. Shilpi Chopra, Ph.D.
Gas Chromatograph Mass Spectrometer No. GCMS-1603 Analysis of Organophosphorus Pesticides in Milk Using SPME and GC-MS/MS Shilpi Chopra, Ph.D. Introduction Organophosphorus (OP) pesticides are a class
More informationEXPERIMENT 1 - Determination of the purity and identity of organic compounds by melting point and/or analytical thin layer chromatography
EXPERIMENT 1 - Determination of the purity and identity of organic compounds by melting point and/or analytical thin layer chromatography PART A Melting points and mixed melting points. As discussed in
More informationChemical analysis service, Turner s Green Technology Group
Chemical analysis service, Turner s Green Technology Group Hourly costs, Academic collaborations leading to co-publications: 627 SEK/hr (excl. VAT) Hourly costs, Industry/Academic collaborations, no publications:
More informationDetermination of Pesticide Residues in Drinking Water Using Automated Solid-Phase Extraction and Gas Chromatography with Nitrogen Phosphorus Detection
Determination of Pesticide Residues in Drinking Water Using Automated Solid-Phase Extraction and Gas Chromatography with Nitrogen Phosphorus Detection Application Note 1097 Liu Qian, Zheng Hongguo and
More informationPaper Chromatography: Separation and Identification of Five Metal Cations
Paper Chromatography: Separation and Identification of Five Metal Cations Objectives Known and unknown solutions of the metal ions Ag +, Fe 3+, Co 2+, Cu 2+ and Hg 2+ will be analyzed using paper chromatography.
More informationThe Theory of HPLC. Gradient HPLC
The Theory of HPLC Gradient HPLC i Wherever you see this symbol, it is important to access the on-line course as there is interactive material that cannot be fully shown in this reference manual. Aims
More informationChapter 21 Principles of Chromatography and Mass Spectrometry Problems 1,2,3,4,8,9,11,13
Chapter 21 Principles of Chromatography and Mass Spectrometry Problems 1,2,3,4,8,9,11,13 21-1 What is Chromatography Process to separate and purify compounds in a mixture by passing them trough a material
More informationDaniel M. Mueller, Katharina M. Rentsch Institut für Klinische Chemie, Universitätsspital Zürich, CH-8091 Zürich, Schweiz
Toxichem Krimtech 211;78(Special Issue):324 Online extraction LC-MS n method for the detection of drugs in urine, serum and heparinized plasma Daniel M. Mueller, Katharina M. Rentsch Institut für Klinische
More information9.2.3.7 Retention Parameters in Column Chromatography
9.2.3.7 Retention Parameters in Column Chromatography Retention parameters may be measured in terms of chart distances or times, as well as mobile phase volumes; e.g., t R ' (time) is analogous to V R
More informationRECITATION NOTES FOR EXPERIMENT # 5 A&B THIN LAYER CHROMATOGRAPHY
RECITATION NOTES FOR EXPERIMENT # 5 A&B THIN LAYER CHROMATOGRAPHY Have your lab textbook available for quick reference to specific pages, indicated in red. BASIC PRINCIPLES OF CHROMATOGRAPHY Chromatography
More informationSUCRALOSE. White to off-white, practically odourless crystalline powder
SUCRALOSE Prepared at the 41st JECFA (1993), published in FNP 52 Add 2 (1993). Metals and arsenic specifications revised at the 63rd JECFA (2004). An ADI of 0-15 mg/kg bw was established at the 37th JECFA
More informationGOED VOLUNTARY MONOGRAPH (v. 4)
GOED VOLUNTARY MONOGRAPH (v. 4) Omega-3 EPA Omega-3 DHA Omega-3 EPA & DHA DEFINITION Omega-3 fatty acids, EPA and DHA, consist of the all cis forms of 5, 8, 11, 14, 17-eicosapentaenoic acid and 4, 7, 10,
More informationReactions of Fats and Fatty Acids
Reactions of Fats and Fatty Acids Outline Fats and Oils Fatty Acid Biosynthesis Biodiesel Homework We hear quite a lot about the place of fats and oils in human nutrition. Foods high in fat are at the
More informationHPLC Basics, Equipment, and Troubleshooting
HPLC Basics, Equipment, and Troubleshooting HPLC Basics, Equipment, and Troubleshooting explains chromatography in practical terms from the ground up HPLC Basics, Equipment, and Troubleshooting HPLC Basics,
More informationFOR RISK ASSESSMENT. Analysis of mineral oil: Manual pre-separation, followed by LV-GC/FID Off-line HPLC-GC/FID FEDERAL INSTITUTE.
FEDERAL INSTITUTE FOR RISK ASSESSMENT Analysis of mineral oil: Manual pre-separation, followed by LV-GC/FID Off-line HPLC-GC/FID Christoph Hutzler Topics Design of the manual methods Large Volume GC injections
More informationThe First Quantitative Analysis of Alkylated PAH and PASH by GCxGC/MS and its Implications on Weathering Studies
The First Quantitative Analysis of Alkylated PAH and PASH by GCxGC/MS and its Implications on Weathering Studies INEF Penn State Conference 2013 Albert Robbat, Jr. and Patrick Antle Tufts University, Chemistry
More informationFor Chromatography, you must remember Polar Dissolves More, not like dissolves like.
Chromatography In General Separation of compounds based on the polarity of the compounds being separated Two potential phases for a compound to eist in: mobile (liquid or gas) and stationary Partitioning
More informationGeneral aspects of GC analysis of volatiles
General aspects of GC analysis of volatiles Kaunas University of Technology Content Introduction: food volatiles, principles of GC separation Main parameters for the optimisation of volatile analysis columns
More informationTransformer Oil Gas Analysis
Get on the Analyzer FAST TRACK Transformer Oil Gas Analysis Helping customers protect the environment! 1 Why Analyzers Value to Customers Alleviates Resource Pressure Facilitates Configuration Use Advanced
More informationAnalysis of Free Bromate Ions in Tap Water using an ACQUITY UPLC BEH Amide Column
Analysis of Free Bromate Ions in Tap Water using an ACQUITY UPLC BEH Amide Column Sachiki Shimizu, FUJIFILM Fine Chemicals Co., Ltd., Kanagawa, Japan Kenneth J. Fountain, Kevin Jenkins, and Yoko Tsuda,
More informationEnhancing GCMS analysis of trace compounds using a new dynamic baseline compensation algorithm to reduce background interference
Enhancing GCMS analysis of trace compounds using a new dynamic baseline compensation algorithm to reduce background interference Abstract The advantages of mass spectrometry (MS) in combination with gas
More informationMETHOD 8082A. POLYCHLORINATED BIPHENYLS (PCBs) BY GAS CHROMATOGRAPHY
METHOD 8082A POLYCHLORINATED BIPHENYLS (PCBs) BY GAS CHROMATOGRAPHY SW-846 is not intended to be an analytical training manual. Therefore, method procedures are written based on the assumption that they
More information5s Solubility & Conductivity
5s Solubility & Conductivity OBJECTIVES To explore the relationship between the structures of common household substances and the kinds of solvents in which they dissolve. To demonstrate the ionic nature
More informationHolcim EMR List of EN Standards and VDI Guidelines usable for Discontinuous Measurements in Cement Plants
Holcim EMR List of EN Standards and VDI Guidelines usable for Discontinuous Measurements in Cement Plants Version 2004-01 / August 2004 (HGRS-JW-04-25s) HGRS-CTS/MT J. Waltisberg SUMMARY ( ) shall not
More informationSULFUR COMPOUNDS IN PETROLEUM HYDROCARBON STREAMS
Petroleum & Coal ISSN 1337-7027 Available online at www.vurup.sk/petroleum-coal Petroleum & Coal 54 (1) 9-13, 2012 SULFUR COMPOUNDS IN PETROLEUM HYDROCARBON STREAMS Antoaneta Pavlova, Pavlina Ivanova,
More informationIntelligent use of Relative Response Factors in Gas Chromatography-Flame Ionisation Detection
52 May/June 2012 Intelligent use of Relative Response Factors in Gas Chromatography-Flame Ionisation Detection by Karen Rome and Allyson McIntyre, AstraZeneca, Macclesfield, SK10 2NA, UK Quantitative analysis
More informationFipronil Analysis By GC/XSD following Post-Extraction Gel Permeation Chromatography Cleanup
Application Note 25570306 Fipronil Analysis By GC/XSD following Post-Extraction Gel Permeation Chromatography Cleanup Keywords Gel Permeation Chromatography GPC AutoPrep 2000 Pesticides RapidVap N2 System
More informationPurification of reaction mixtures using flash chromatography.
Purification of reaction mixtures using flash chromatography. This technical note details the use of ISOLUTE Flash chromatography columns for the purification of reaction mixtures. What is flash chromatography?
More informationBIOLOGICAL MEMBRANES: FUNCTIONS, STRUCTURES & TRANSPORT
BIOLOGICAL MEMBRANES: FUNCTIONS, STRUCTURES & TRANSPORT UNIVERSITY OF PNG SCHOOL OF MEDICINE AND HEALTH SCIENCES DISCIPLINE OF BIOCHEMISTRY AND MOLECULAR BIOLOGY BMLS II / B Pharm II / BDS II VJ Temple
More informationSPE, LC-MS/MS Method for the Determination of Ethinyl Estradiol from Human Plasma
SPE, LC-MS/MS Method for the Determination of Ethinyl Estradiol from uman Plasma Krishna Rao Dara, Dr. Tushar N. Mehta, Asia Pacific Center of Excellence, Thermo Fisher Scientific, Ahmedabad, India Application
More informationRapid Screening Method for Illicit Drugs, Using an Advanced Solid Core UHPLC Column and UHPLC System with MS/MS Detection
Rapid Screening Method for Illicit Drugs, Using an Advanced Solid Core UHPLC Column and UHPLC System with MS/MS Detection Derek Hillbeck, Thermo Fisher Scientific, Runcorn, UK Application Note 2999 Key
More informationß-CYCLODEXTRIN SYNONYMS
ß-CYCLODEXTRIN Prepared at the 44th JECFA (1995), published in FNP 52 Add 3 (1995) superseding specifications prepared at the 41st JECFA (1993), published in FNP 52 Add 2 (1993). Metals and arsenic specifications
More informationCannabis Analysis: Potency Testing Identification and Quantification of THC and CBD by GC/FID and GC/MS
APPLICATION NOTE Gas Chromatography/ Mass Spectrometry Authors: Timothy D. Ruppel Nathaniel Kuffel PerkinElmer, Inc. Shelton, CT Cannabis Analysis: Potency Testing Identification and Quantification of
More information