Corso Vittorio Emanuele 494, Napoli (Italy)
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1 PERSONAL INFORMATION Simona Cataldi Corso Vittorio Emanuele 494, Napoli (Italy) Sex Female Date of birth 8 Sep 1990 Nationality Italian Scopus ID: ORCID ID: Official H- index: 2 WORK EXPERIENCE Mar 2018 Jun 2018 Nov 2015 Dec 2018 Ph.D visiting student Centre Méditerranéen de Médecine Moléculaire (C3M), Nice (France) Short term research stay in the laboratory of Cellular and Molecular Physiopathology of Obesity coordinated by Dr. Jean- François Tanti. Ph.D student Institute of Genetics and Biophysics A. Buzzati-Traverso (IGB), Naples (Italy) Research activities and tutoring of undergraduates. Collaboration to national and international projects in the Human Genetics Diseases Lab coordinated by Prof. Alfredo Ciccodicola. Sep 2012 Oct 2015 Undergraduate student Institute of Genetics and Biophysics A. Buzzati-Traverso (IGB), Naples (Italy) Research and training activities in the Human Genetics Diseases Lab coordinated by Prof. Alfredo Ciccodicola. EDUCATION AND TRAINING Nov 2015 Dec 2018 Oct 2012 May 2015 Ph.D in Biomolecular Sciences Department of Environmental Sciences and Technologies, Biological and Pharmaceutical "DISTABiF" at University of Naples Luigi Vanvitelli., Caserta (Italy) Experimental thesis: A dominant negative PPARγ isoform at the crossroad between adipogenesis and obesity. Coordinator: Prof. Andrea Riccio, University of Naples "Luigi Vanvitelli" Relator: Prof. Alfredo Ciccodicola, Institute of Genetics and Biophysics A. Buzzati- Traverso, CNR, Naples; Department of Genetics, Parthenope University of Naples. Master Degree in Biological Science (Curriculum in Molecular Diagnostic 110/110 full marks) Faculty of MM.FF.NN, Federico II University of Naples, Naples (Italy) Experimental thesis in Human Genetic: Potential Role of a PPARγ dominant negative isoform on gene expression in human adipose tissue. European Union, Page 1 / 6
2 Relator Prof. Luciano Gaudio, Department of Genetics, Federico II University of Naples. Co-relator: Prof. Alfredo Ciccodicola, Institute of Genetics and Biophysics A. Buzzati- Traverso, CNR, Naples; Department of Genetics, Parthenope University of Naples. Oct 2009 May 2012 Bachelor Degree in Biological Science (110/110 full marks) Faculty Science and Technology University of Naples Parthenope, Naples (Italy) Experimental thesis in Human Genetic: Analysis of new genes isoforms involved in inflammation. Relator Prof. Alfredo Ciccodicola, Institute of Genetics and Biophysics A. Buzzati- Traverso, CNR, Naples; Department of Genetics, Parthenope University of Naples. Sep 2004 Jul 2009 High school degree (78/100) Liceo Ginnasio Statale Giambattista Vico, Naples (Italy) PERSONAL SKILLS Mother tongue(s) Italian Foreign language(s) UNDERSTANDING SPEAKING WRITING Listening Reading Spoken interaction Spoken production English B2 C1 B2 C1 B2 Levels: A1 and A2: Basic user - B1 and B2: Independent user - C1 and C2: Proficient user Common European Framework of Reference for Languages Communication, social and organizational skills Dr. Cataldi shows excellent ability to work under pressure. Her research and training activities helped her to develop high propensity to solve problems and reach relevant results despite the difficulties. Moreover, she has acquired a high propensity to teamwork and scientific collaborations. In addition, she gained organizational skills, decision-making and responsibilities in coordination of scientific work of undergraduate students. Dr. Cataldi is strongly self motivated and she can complete a task, even when challenging, without giving up. She shows a great communication skill acquired through research training at home institution and abroad, collaborations, attending National and International meetings, and thanks to the collaboration with Prof. Alfredo Ciccodicola in teaching and mentoring activities at the Genetics and Molecular Biology courses at University Parthenope, Naples. Job-related skills Since she was an undergraduate student, Dr. Cataldi was involved in projects regarding the characterization and the study of alternative splicing isoforms of human transcripts. In particular, during her master degree and Ph.D training at the CNR in Naples, she focused on the study of the role of dominant negative splice isoforms of PPARG gene in the context of obesity and metabolic complications. During this project she acquired different skills in cellular and molecular biology, well documented in the publications in which she is involved. She acquired competences about the generation of cdna libraries for NGS and basic skills in the analysis of RNA-Seq data. Moreover, she actively contributed to set up a model of human adipocyte and hypertrophic adipocyte differentiation from human adipose derived immortalized mesenchymal stem cells. She characterized the model from the morphologic and genes/proteins expression point of view. Thanks to the experience in the laboratory of Jean- François Tanti in Nice she nourished ideas through exposure to different methods and scientific skills. She had the opportunity to acquire technical and theoretical knowledge about the relationship between inflammation and metabolic diseases. She learned how to induce mouse and human in vitro macrophages differentiation. Moreover, she started a collaboration with Dr. Jean-François Lout to set up a protocol of brown adipocyte differentiation from human adipose European Union, Page 2 / 6
3 derived immortalized mesenchymal stem cells. In addition, Dr. Cataldi expanded and improved her knowledge about metabolic processes and genes involved therein participating at International meeting such as European Association for the Study of Diabetes annual meeting and International Symposium on Insulin Receptor and Insulin Action. Digital skills SELF-ASSESSMENT Information processing Communication Content creation Safety Problemsolving Independent user Independent user Independent user Basic user Basic user Digital skills - Self-assessment grid ECDL Operative systems used: Windows and Mac. Excellent use of Office softwares (Word, PowerPoint and Excel) Excellent use of main Internet Browsers: Mozilla Firefox, Internet Explorer, Safari and Netscape. Excellent use of Mac softwares for nucleotide and aminoacid sequences analysis. Excellent use of softwares for editing of digital pictures Basic use of R Graphics Package Publications Aprile M, Cataldi S, Ambrosio MR, D'Esposito V, Lim K, Dietrich A, Blüher M, Savage DB, Formisano P, Ciccodicola A, Costa V. PPARγΔ5, a Naturally Occurring Dominant-Negative Splice Isoform, Impairs PPARγ Function and Adipocyte Differentiation. Cell Reports Nov 6;25(6): e6. doi: /j.celrep PubMed PMID: Cataldi S, Aprile M, Italiani P, Ciccodicola A, Costa V. The impact of inflammation on alternative splicing at PPARG locus in hypertrophic obesity. Published Abstract, Diabetologia 61, S255-S255. Aprile M, Cataldi S, Bluher M, Ciccodicola A, Costa V. New insights in adipose tissue dysfunctions in diabetic obese patients: a new PPARγ truncated isoform mimicking PPARγ dominant negative mutations. Published Abstract, Diabetologia 61, S111-S111. Aprile M, Ambrosio MR, Cataldi S, D'Esposito V, Terracciano D, Formisano P, Ciccodicola A, Costa V. A new PPARγ truncated isoform induced by a regulatory feedback: is alternative splicing crucial in adipocyte differentiation and defective neoadipogenesis? Published Abstract, Diabetologia 59, pp. S88-S89. Costa V, Federico A, Pollastro C, Ziviello C, Cataldi S, Formisano P, Ciccodicola A. Computational Analysis of Single Nucleotide Polymorphisms Associated with Altered Drug Responsiveness in Type 2 Diabetes. Int J Mol Sci Jun 25;17(7). pii: E1008. doi: /ijms PubMed PMID: ; PubMed Central PMCID: PMC Honours and awards Awarded from the EASD of the Albert Renold Travel Fellowship to work in the Cellular and Molecular Physiopathology of Obesity Lab coordinated by Dr. Jean- François Tanti at Centre Méditerranéen de Médecine Moléculaire (C3M) in Nice. To be accomplished in Awarded from EASD of the EASD Travel Grant to attend the 54 rd EASD Annual Meeting in Berlin, Germany. October Awarded from EMBO of the 3-months Short Term Fellowship to carry out research activities during the PhD training in the Cellular and Molecular Physiopathology of Obesity Lab coordinated by Dr. Jean-François Tanti at Mediterranean Centre for Molecular Medicine European Union, Page 3 / 6
4 (C3M) in Nice. March- June Poster session/ Abstracts Cataldi S et al., The impact of inflammation on alternative splicing at PPARG locus in hypertrophic obesity. Poster Presentation at 54th Annual Meeting of the European Association for the Study of Diabetes (EASD), Berlin, Germany, October Aprile M, Cataldi S, et al. New insights in adipose tissue dysfunctions in diabetic obese patients: a new PPARγ truncated isoform mimicking PPARγ dominant negative mutations. Oral presentation at 54th Annual Meeting of the European Association for the Study of Diabetes (EASD), Berlin, Germany, October Cataldi S et al., Regulation of PPARγ beyond genetic mutation: the impact of the alternative splicing and dominant negative isoforms. Poster at XIX National Congress SIGU, Turin, Italy, November Aprile M, Ambrosio MR, Cataldi S et al. A new PPARγ truncated isoform induced by a regulatory feedback: is alternative splicing crucial in adipocyte differentiation and defective neoadipogenesis?. Oral presentation at 52th Annual Meeting of the European Association for the Study of Diabetes (EASD), Munich, Germany, September Cataldi S et al., Defective adipocyte differentiation beyond genetic mutation: regulation of PPARγ through alternative splicing. Poster at National Ph.D. Meeting, Salerno, 7-9 April, Courses/Meeting 54th Annual Meeting of the European Association for the Study of Diabetes (EASD). 1-5 October 2018, Berlin, Germany. Symposium Inflammation and Disease April 2018, Nice, France. International Symposium on Insulin Receptor and Insulin Action April 2017, Nice, France. XIX National Congress SIGU, November 2016, Turin, Italy. 52th Annual Meeting of the European Association for the Study of Diabetes (EASD) September 2016, Munich, Germany. HORIZON 2020 il nuovo programma quadro in ricerca e innovazione: opportunità per il tema salute scrittura di una proposta di successo. 4 May 2016, Naples, Italy National Ph.D. Meeting. 7-9 April 2016, Salerno, Italy. Chromatin, Epigenome And Drug Discovery Training School March 2016, Naples, Italy. Basic R October 2015, Open Bionformatics Laboratory, CNR-IGB, Via Pietro Castellino, 111. Ecdl. 8 April 2008, Liceo Ginnasio statale Giambattista Vico, Via Salvator Rosa, 117. English Course. 1 8 March 2008, Anglo European Study Tours Group LTD (AEST), London. ADDITIONAL INFORMATION Wet laboratory skills Main molecular biology procedures acquired: Cultures of different human and mouse cell lines such as primary human white adipocytes and SVF, adipose derived human mesenchymal stem cells, THP-1, 3T3-L1, RAW Direct and indirect co-cultures between two different cell lines Pharmacological treatments in vitro Transient and stable transfection of human cell lines In vitro induction of human and mouse adipocyte differentiation In vitro generation of human hypertrophic adipocytes European Union, Page 4 / 6
5 In vitro differentiation of human and mouse macrophages Lipids staining and quantification by Oil Red O Lipolysis assay Cell staining for immunofluorescence microscopy RNA, DNA and proteins extraction from human and mouse cells and tissues biopsies Gene-specific expression assays semi- and quantitative RT-PCR DNA/RNA gel electrophoresis DNA/cDNA cloning in expression vectors DNA Methylation Analysis (bisulphite treatment and targeted sequencing) Transformation of bacteria with plasmids Plasmid DNA extraction (Mini, Midi and Maxi Prep) Endonuclease digestion Western blot Luciferase assay Sulforhodamine B assay Cell-cycle analysis by flow cytometry In vitro scratch assay for cell migration analysis PolyA+ mrna selection for RNA-Seq on next-generation sequencing platforms (Illumina), including: - RNA isolation and purification - poly(a+) enrichment on total RNA samples -Paired-end library preparation for next-generation sequencing - Quality control of RNA and library integrity by Experion system (BioRad) Bioinformatic tools Main nucleotide and protein Databases: NCBI, Unigene, UniSTS, dbest, dbsnp, RNAdB, GeneBank, GeneCards, AceView, EMBL Bank, HGNC For UniProt, SWISSPROT, Ensemble. Database and Genome Browsers: BLAST (NCBI), BLAT (UCSC). Genome Browser (UCSC), Integrated Genome Viewer (IGV). Tools/databases for NGS analysis: RNA-SeqGUI, Galaxy, TCGA portal, GTeX, COSMIC, cbioportal. Other bioinformatics tools for secondary analysis: David, Panther, KEGG, KEGG mapper, STRING, G-profiler. Driving licence B La sottoscritta, Simona Cataldi, nata a Napoli il 08/09/1990, consapevole delle sanzioni penali richiamate dall'art. 76 del D.P.R. 28/12/00 n. 445 in caso di dichiarazioni mendaci e della decadenza dei benefici eventualmente conseguenti al provvedimento emanato sulla base di dichiarazioni non veritiere, di cui all'art. 47 del D.P.R. del 28/12/00 n. 445, ai sensi e per gli effetti dell'art. 47 del citato D.P.R. 445/2000, sotto la propria responsabilità DICHIARA European Union, Page 5 / 6
6 Che i dati e le informazioni del presente curriculum vitae corrispondono al vero, - Di aver preso visione dell Informativa privacy, di cui all art. 13 del. D. Lgs. n.196/2003, inserita sul sito del Ministero degli Affari Esteri ( e nell avviso di selezione. AUTORIZZA il trattamento dei dati personali ai sensi del Decreto Legislativo 30 giugno 2003, n. 196 "Codice in materia di protezione dei dati personali. Napoli, 10/04/2019 Signature European Union, Page 6 / 6
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