Bottlenecks in Clinical Source Material Acquisition. Aby J. Mathew, PhD May 5, 2009 ISCT Annual Meeting San Diego, CA
|
|
- Thomas Terry
- 8 years ago
- Views:
Transcription
1 Bottlenecks in Clinical Source Material Acquisition Aby J. Mathew, PhD May 5, 2009 ISCT Annual Meeting San Diego, CA
2 Biopreservation What s the issue? Biopreservation considerations towards Source Material Acquisition Unmet needs Scientific support for improved biopreservation 2
3 HISTORICAL ASSUMPTIONS Assumes that the cell/tissue is a passive participant Assumes that the effects of the sub lethal stresses are not additive or reversible Assumes cell in = cell out Assumes that Perceived Viability immediately post preservation = True Viability long term 3
4 BIOPRESERVATION YIELD THE PROBLEM! Cascading yield effects common to nearly all biopreservation applications Outcomes heavily affected by lack of in process efficiencies and optimization Does the beginning affect the end? Where does my cell therapy end up? 4
5 Low yield (viability and function) of source material and manufactured products often from current preservation technologies and processes Limited shelf life of source material and finished products Quality Concerns Need to reduce or eliminate toxic components Need for defined media with few/no animal components Varied/misleading results from assays and time of testing Impact of preservation process on outcomes Biopreservation media solutions are excipients/reagents in Cell Therapy Has your commercial media or self formulated cocktail been fully qualified in your process? Does your preservation solution actually work? Is your cell therapy product really as effective (including cost effective) as you want? Risk Management Suboptimal biopreservation affects each phase of development Source Material Acquisition Transportation Logistics R&D Cell Banking Therapeutic Delivery Clinical Efficacy Cost Effectiveness POINTS TO CONSIDER 5
6 BIOPRESERVATION: UNMET NEEDS & OPTIMIZATION GOALS Extending shelf life, improving transportation logistics Increasing post preservation recoverable yield Increasing post preservation functional yield Improving biopreservation media quality Improving efficiency in media prep Source material 6
7 RECENT STUDY IN CYTOTHERAPY
8 Cellular responses to low temperature what happens? Metabolic activity decreases Ionic and osmotic balance is disrupted reduced membrane pump activity Free radicals accumulate Molecular stress responses are initiated Initiation of apoptotic cell death signaling pathways Secondary necrotic injury (necroptosis) CELLULAR RESPONSE TO COLD Multiple points for preservation stress (hypothermia, ischemia, postpreservation) Cellular priorities are survival, recovery, then functional performance 8
9 Adjustment of Na, K, Ca, Mg ratios to restrict passive ion movement FORMULATING A SOLUTION Osmotic Support impermeant anion to replace Cl in the extracellular space and balance fixed intracellular ions Chelators buffer calcium, iron dysregulation ph Buffering Capacity specific to low temperature (15ºC to 2ºC) Free Radical Scavengers Energy substrates but still serum free! Inhibition of preservation induced Apoptosis and Necrosis Intracellular like vs. Extracellular like 9
10 MOLECULAR ORIGIN OF CELL DEATH Mathew et al.,
11 IMPROVED HYPOTHERMIC STORAGE Media HTS FRS 11
12 EXPANDED PRESERVATION WINDOW 24 Hour 48 Hour 12
13 Biopreservation solutions are considered Excipients Not a Drug Not a Medical Device Is it cgmp Manufactured? Are there FDA Master Files available for cross reference? Does it have Fully Defined Components and Finished Product? Protein Free Serum Free USP/Multicompendial/Highest quality components QUALITY AND REGULATORY Elevate the bar for Biopreservation solutions 13
14 VISUAL EVIDENCE 5 Days Storage of Human Fibroblasts at 2 8ºC and 1 Day Recovery at 37ºC NHDF Cells Stored in HypoThermosol NHDF Cells Stored in Viaspan NHDF Cells Stored in Celsior Green = Actin Cytoskeleton Red = Mitochondria Activation Blue = Nuclear Stain 14
15 WHAT DO YOUR CELLS LOOK LIKE? 24 hr storage at 2-8ºC in intracellular-like HypoThermosol 24 hr storage at 2-8ºC in traditional media Human Keratinocytes stained for mitochondrial activity (red) and cytoskeletal integrity (green) Snyder et al.,
16 16
Fighting the Battles: Conducting a Clinical Assay
Fighting the Battles: Conducting a Clinical Assay 6 Vocabulary: In Vitro: studies in biology that are conducted using components of an organism that have been isolated from their usual biological surroundings
More informationTransmembrane proteins span the bilayer. α-helix transmembrane domain. Multiple transmembrane helices in one polypeptide
Transmembrane proteins span the bilayer α-helix transmembrane domain Hydrophobic R groups of a.a. interact with fatty acid chains Multiple transmembrane helices in one polypeptide Polar a.a. Hydrophilic
More informationTeriflunomide is the active metabolite of Leflunomide, a drug employed since 1994 for the treatment of rheumatoid arthritis (Baselt, 2011).
Page 1 of 10 ANALYTE NAME AND STRUCTURE TERIFLUNOMIDE Teriflunomide TRADE NAME Aubagio CATEGORY Antimetabolite TEST CODE PURPOSE Therapeutic Drug Monitoring GENERAL RELEVANCY BACKGROUND sclerosis. The
More informationThe bleeding edge: emerging issues in biobankingand cryopreservation Allison Hubel, PhD University of Minnesota Preservation as a platform technology Food Core scientific principals Mechanisms of degradation
More informationAAGPs TM Anti-Aging Glyco Peptides. Enhancing Cell, Tissue and Organ Integrity Molecular and biological attributes of lead AAGP molecule
AAGPs TM Anti-Aging Glyco Peptides Enhancing Cell, Tissue and Organ Integrity Molecular and biological attributes of lead AAGP molecule 1 Acknowledgements This presentation was prepared by Dr. Samer Hussein
More informationModes of Membrane Transport
Modes of Membrane Transport Transmembrane Transport movement of small substances through a cellular membrane (plasma, ER, mitochondrial..) ions, fatty acids, H 2 O, monosaccharides, steroids, amino acids
More informationCompound extracted from plant Aristolochia. Nephrotoxin and carcinogen. Page 2
Mariana Babayeva MD, PhD Touro College of Pharmacy, New York, NY, USA Page 1 Compound extracted from plant Aristolochia Nephrotoxin and carcinogen Page 2 AA-I is an organic anion eliminated by the kidney
More informationBioPreservation Today
BioPreservation Today Volume 1 - Issue 4 FALL 2009 published by BioLife Solutions, Inc. FEATURE ARTICLE CASE STUDY: MODIFIED CRYOPRESERVATION OF UMBILICAL CORD BLOOD STEM CELLS AT CRYOBANKS INTERNATIONAL
More informationInvestor Presentation NASDAQ:BLFS
Investor Presentation NASDAQ:BLFS Safe Harbor Statement This presentation contains forward-looking statements, including, but not limited to, statements concerning the company s anticipated business and
More informationThe Need for a PARP in vivo Pharmacodynamic Assay
The Need for a PARP in vivo Pharmacodynamic Assay Jay George, Ph.D., Chief Scientific Officer, Trevigen, Inc., Gaithersburg, MD For further infomation, please contact: William Booth, Ph.D. Tel: +44 (0)1235
More informationIslet Viability Assessment by Single Cell Flow Cytometry
Islet Viability Assessment by Single Cell Flow Cytometry Page 1 of 8 Purpose: To comprehensively assess the viability of the islet cell preparation prior to transplantation. Tissue Samples: A sample containing
More informationEarly successes of cell therapy
B IOP ROCESSTECHNICAL Hypothermic Storage and Cryopreservation Successful Short- and Long-Term Preservation of Cells and Tissues Robert G. Van Buskirk, John M. Baust, Kristi K. Snyder, Aby J. Mathew, and
More informationIII. Cell destruction by freezing and cryoprobe development
III. Cell destruction by freezing and cryoprobe development 1 WHAT IS CRYOSURGERY? Cryoprobe Surface iceball formation Ice-ball Cryoprobe Cryosurgery of liver (Angular insertion) Cryosurgery of liver (Vertical
More informationApplication Note No. 2 / July 2012. Quantitative Assessment of Cell Quality, Viability and Proliferation. System
Application Note No. 2 / July 2012 Quantitative Assessment of Cell Quality, Viability and Proliferation System Quantitative Assessment of Cell Quality, Viability and Proliferation Introduction In vitro
More informationBIOLIFE SOLUTIONS INC
SECURITIES & EXCHANGE COMMISSION EDGAR FILING BIOLIFE SOLUTIONS INC Form: 8-K Date Filed: 2016-01-07 Corporate Issuer CIK: 834365 Copyright 2016, Issuer Direct Corporation. All Right Reserved. Distribution
More informationThe Impact of Polyphenolic Compounds on Apoptosis in Epithelial Cells
The Impact of Polyphenolic Compounds on Apoptosis in Epithelial Cells Mayson Husband, 12 th grade, Latta High School Abstract The purpose of this investigation was to determine whether polyphenolic compounds
More informationBiology Institute: 7 PhD programs Expertise in all areas of biological sciences
Biology Institute: 7 PhD programs Expertise in all areas of biological sciences!" #$%&'()*" '+**$,%' Biology Institute: PhD programs Programs Website: http://www.ib.unicamp.br/pos About the Biology Institute
More informationHemaCare Corporation Company Overview. Comprehensive Product and Service Solutions for BioResearch and Cellular Therapy
HemaCare Corporation Company Overview Comprehensive Product and Service Solutions for BioResearch and Cellular Therapy 2015 HemaCare Corporation Leading provider of apheresis products, human blood cells,
More informationFEATURE ARTICLE BIOLIFE PRODUCTS IN REGEN MED CLINICAL TRIALS. Volume 4 - Issue 2 Winter 2013 BIOLIFE SOLUTIONS CONTRACT MEDIA MANUFACTURING
Volume 4 - Issue 2 Winter 2013 PHACILITATE S 9TH ANNUAL CELL & GENE THERAPY FORUM WASHINGTON, DC FEATURE ARTICLE BIOLIFE PRODUCTS IN REGEN MED CLINICAL TRIALS BIOLIFE SOLUTIONS CONTRACT MEDIA MANUFACTURING
More informationRegulating the Internal Environment Water Balance & Nitrogenous Waste Removal
Regulating the Internal Environment Water Balance & Nitrogenous Waste Removal 2006-2007 Animal systems evolved to support multicellular life CH CHO O 2 O 2 NH 3 CH CHO O 2 CO 2 NH NH 3 O 2 3 NH 3 intracellular
More informationTodays Outline. Metabolism. Why do cells need energy? How do cells acquire energy? Metabolism. Concepts & Processes. The cells capacity to:
and Work Metabolic Pathways Enzymes Features Factors Affecting Enzyme Activity Membrane Transport Diffusion Osmosis Passive Transport Active Transport Bulk Transport Todays Outline -Releasing Pathways
More informationLEUPHASYL A NEW PENTAPEPTIDE FOR EXPRESSION WRINKLES CODE: PD080. Date: June 2005 Revision: 1 A GMP PEPTIDE FOR COSMETIC APPLICATIONS
Polígon Industrial Camí Ral C/Isaac Peral, 17 08850 Gavá Barcelona - Spain Telf. +34 93 638 80 00 fax. +34 93 638 93 93 Internet: www.lipotec.com e-mail. commercial@lipotec.com abcde LEUPHASYL A NEW PENTAPEPTIDE
More informationMembrane Structure and Function
Membrane Structure and Function Part A Multiple Choice 1. The fluid mosaic model describes membranes as having A. a set of protein channels separated by phospholipids. B. a bilayer of phospholipids in
More informationTotal body water ~(60% of body mass): Intracellular fluid ~2/3 or ~65% Extracellular fluid ~1/3 or ~35% fluid. Interstitial.
http://www.bristol.ac.uk/phys-pharm/teaching/staffteaching/sergeykasparov.htmlpharm/teaching/staffteaching/sergeykasparov.html Physiology of the Cell Membrane Membrane proteins and their roles (channels,
More informationBIOLOGICAL MEMBRANES: FUNCTIONS, STRUCTURES & TRANSPORT
BIOLOGICAL MEMBRANES: FUNCTIONS, STRUCTURES & TRANSPORT UNIVERSITY OF PNG SCHOOL OF MEDICINE AND HEALTH SCIENCES DISCIPLINE OF BIOCHEMISTRY AND MOLECULAR BIOLOGY BMLS II / B Pharm II / BDS II VJ Temple
More informationapoptosis detection Apoptosis assays for the Attune Acoustic Focusing Cytometer Apoptosis detection Attune Acoustic Focusing Cytometer
apoptosis detection ttune coustic Focusing Cytometer poptosis detection poptosis assays for the ttune coustic Focusing Cytometer poptosis is a carefully regulated process of cell death that occurs as a
More informationChapter 2: Cell Structure and Function pg. 70-107
UNIT 1: Biochemistry Chapter 2: Cell Structure and Function pg. 70-107 Organelles are internal structures that carry out specialized functions, interacting and complementing each other. Animal and plant
More informationAnimal Cell Culture. Third Edition. A Practical Approach OXJORD VNIVVRSITY 1'RVSS
Animal Cell Culture Third Edition A Practical Approach Edited by John R. W. Masters 3rd Floor Research Laboratories, University College London OXJORD VNIVVRSITY 1'RVSS Contents List of protocols page xiii
More informationCELL MEMBRANES, TRANSPORT, and COMMUNICATION. Teacher Packet
AP * BIOLOGY CELL MEMBRANES, TRANSPORT, and COMMUNICATION Teacher Packet AP* is a trademark of the College Entrance Examination Board. The College Entrance Examination Board was not involved in the production
More informationBi 360: Midterm Review
Bi 360: Midterm Review Basic Neurobiology 1) Many axons are surrounded by a fatty insulating sheath called myelin, which is interrupted at regular intervals at the Nodes of Ranvier, where the action potential
More informationNotch 1 -dependent regulation of cell fate in colorectal cancer
Notch 1 -dependent regulation of cell fate in colorectal cancer Referees: PD Dr. Tobias Dick Prof. Dr. Wilfried Roth http://d-nb.info/1057851272 CONTENTS Summary 1 Zusammenfassung 2 1 INTRODUCTION 3 1.1
More informationMechanism of short-term ERK activation by electromagnetic fields at mobile phone frequencies. Biochemistry Journal. August 1, 2007 405, pp.
Mechanism of short-term ERK activation by electromagnetic fields at mobile phone frequencies 1 Biochemistry Journal August 1, 2007 405, pp. 559 568 Joseph Friedman, Sarah Kraus, Yirmi Hauptman, Yoni Schiff
More informationUnit 2: Cells, Membranes and Signaling CELL MEMBRANE. Chapter 5 Hillis Textbook
Unit 2: Cells, Membranes and Signaling CELL MEMBRANE Chapter 5 Hillis Textbook HOW DOES THE LAB RELATE TO THE NEXT CHAPTER? SURFACE AREA: the entire outer covering of a cell that enables materials pass.
More informationuse. 3,5 Apoptosis Viability Titration Assays
Introduction Monitoring cell viability can provide critical insights into the effectiveness of biological protocols and the stability of biological systems including: the efficacy of cell harvesting procedures,
More informationThe Lipid Bilayer Is a Two-Dimensional Fluid
The Lipid Bilayer Is a Two-Dimensional Fluid The aqueous environment inside and outside a cell prevents membrane lipids from escaping from bilayer, but nothing stops these molecules from moving about and
More informationDate: Student Name: Teacher Name: Jared George. Score: 1) A cell with 1% solute concentration is placed in a beaker with a 5% solute concentration.
Biology Keystone (PA Core) Quiz Homeostasis and Transport - (BIO.A.4.1.1 ) Plasma Membrane, (BIO.A.4.1.2 ) Transport Mechanisms, (BIO.A.4.1.3 ) Transport Facilitation Student Name: Teacher Name: Jared
More informationLecture 8. Protein Trafficking/Targeting. Protein targeting is necessary for proteins that are destined to work outside the cytoplasm.
Protein Trafficking/Targeting (8.1) Lecture 8 Protein Trafficking/Targeting Protein targeting is necessary for proteins that are destined to work outside the cytoplasm. Protein targeting is more complex
More informationBioBusiness Biopharmaceutical Ingredients Recombinant albumins & albumin fusion Dermot Pearson, Strategic Product Director, NZ Biopharma
BioBusiness Biopharmaceutical Ingredients Recombinant albumins & albumin fusion Dermot Pearson, Strategic Product Director, NZ Biopharma 2 Novozymes solutions dedicated to the biopharmaceutical industry
More informationKnipholone anthrone from Kniphofia foliosa induces a rapid onset of necrotic cell death in cancer cells
Knipholone anthrone from Kniphofia foliosa induces a rapid onset of necrotic cell death in cancer cells Fitoterapia, 11/18/2010 Habtemariam S The present study examines the comparative cytotoxicity of
More informationPHOSPHATE-SANDOZ Tablets (High dose phosphate supplement)
1 PHOSPHATE-SANDOZ Tablets (High dose phosphate supplement) PHOSPHATE-SANDOZ PHOSPHATE-SANDOZ Tablets are a high dose phosphate supplement containing sodium phosphate monobasic. The CAS registry number
More informationPROTOCOL. Immunostaining for Flow Cytometry. Background. Materials and equipment required.
PROTOCOL Immunostaining for Flow Cytometry 1850 Millrace Drive, Suite 3A Eugene, Oregon 97403 Rev.0 Background The combination of single cell analysis using flow cytometry and the specificity of antibody-based
More informationDiscontinuous native protein gel electrophoresis
Discontinuous native protein gel electrophoresis Michael Niepmann and Junfeng Zheng Institute of Biochemistry Friedrichstrasse 24 Faculty of Medicine, JustusLIebigUniversity 35392 Giessen, Germany In this
More information2nd MuscleTech Network Workshop. From translational Research to translational Medicine. CONCLUSIONS AND CLOSING REMARKS Dr.
2nd MuscleTech Network Workshop. From translational Research to translational Medicine. CONCLUSIONS AND CLOSING REMARKS Dr. Carles Pedret MUSCLE AND TENDON WORKSHOP HEALTH AND GENERAL POPULATION SPORTS
More informationCellular Calcium Dynamics. Jussi Koivumäki, Glenn Lines & Joakim Sundnes
Cellular Calcium Dynamics Jussi Koivumäki, Glenn Lines & Joakim Sundnes Cellular calcium dynamics A real cardiomyocyte is obviously not an empty cylinder, where Ca 2+ just diffuses freely......instead
More informationEden Biodesign ebook Monoclonal Antibody Production: Building the Platform
Eden Biodesign ebook Monoclonal Antibody Production: Building the Platform CHAPTER 1: Overview CHAPTER 2: Challenges CHAPTER 3: Purification Methodology CHAPTER 4: Results CHAPTER 5: About Eden Biodesign
More informationChapter 7: Membrane Structure and Function
Name Period Concept 7.1 Cellular membranes are fluid mosaics of lipids and proteins 1. The large molecules of all living things fall into just four main classes. Name them. 2. Explain what is meant when
More informationLAB 5 - PLANT NUTRITION. Chemical Ionic forms Approximate dry Element symbol Atomic weight Absorbed by plants tissue concentration
LAB 5 PLANT NUTRITION I. General Introduction All living organisms require certain elements for their survival. Plants are known to require carbon (C), hydrogen (H), oxygen (O), nitrogen (N), phosphorus
More informationBSC 2010 - Exam I Lectures and Text Pages. The Plasma Membrane Structure and Function. Phospholipids. I. Intro to Biology (2-29) II.
BSC 2010 - Exam I Lectures and Text Pages I. Intro to Biology (2-29) II. Chemistry of Life Chemistry review (30-46) Water (47-57) Carbon (58-67) Macromolecules (68-91) III. Cells and Membranes Cell structure
More information8/20/2012 H C OH H R. Proteins
Proteins Rubisco monomer = amino acids 20 different amino acids polymer = polypeptide protein can be one or more polypeptide chains folded & bonded together large & complex 3-D shape hemoglobin Amino acids
More informationOsteoblast Differentiation and Mineralization
Osteoblast Differentiation and Mineralization Application Note Background Osteoblasts are specialized fibroblasts that secrete and mineralize the bone matrix. They develop from mesenchymal precursors.
More informationCell Biology - Part 2 Membranes
Cell Biology - Part 2 Membranes The organization of cells is made possible by membranes. Membranes isolate, partition, and compartmentalize cells. 1 Membranes isolate the inside of the cell from the outside
More informationCHAPTER 6 AN INTRODUCTION TO METABOLISM. Section B: Enzymes
CHAPTER 6 AN INTRODUCTION TO METABOLISM Section B: Enzymes 1. Enzymes speed up metabolic reactions by lowering energy barriers 2. Enzymes are substrate specific 3. The active site in an enzyme s catalytic
More informationELECTROLYTE SOLUTIONS (Continued)
ELECTROLYTE SOLUTIONS (Continued) Osmolarity Osmotic pressure is an important biologic parameter which involves diffusion of solutes or the transfer of fluids through semi permeable membranes. Per US Pharmacopeia,
More informationHomeostasis and Transport Module A Anchor 4
Homeostasis and Transport Module A Anchor 4 Key Concepts: - Buffers play an important role in maintaining homeostasis in organisms. - To maintain homeostasis, unicellular organisms grow, respond to the
More informationSelect the one that is the best answer:
MQ Kidney 1 Select the one that is the best answer: 1) n increase in the concentration of plasma potassium causes increase in: a) release of renin b) secretion of aldosterone c) secretion of H d) release
More informationCopyright 2000-2003 Mark Brandt, Ph.D. 54
Pyruvate Oxidation Overview of pyruvate metabolism Pyruvate can be produced in a variety of ways. It is an end product of glycolysis, and can be derived from lactate taken up from the environment (or,
More information2007 7.013 Problem Set 1 KEY
2007 7.013 Problem Set 1 KEY Due before 5 PM on FRIDAY, February 16, 2007. Turn answers in to the box outside of 68-120. PLEASE WRITE YOUR ANSWERS ON THIS PRINTOUT. 1. Where in a eukaryotic cell do you
More informationLab 1: Simulation of Resting Membrane Potential and Action Potential
Lab 1: Simulation of Resting Membrane Potential and Action Potential Overview The aim of the present laboratory exercise is to simulate how changes in the ion concentration or ionic conductance can change
More informationCryoinjury of Breast Cancer Cells in Tissue Equivalent Constructs
Cryoinjury of Breast Cancer Cells in Tissue Equivalent Constructs Bumsoo Han Department of Mechanical and Aerospace Engineering University of Texas at Arlington Cryosurgery Minimally invasive surgical
More informationCell Injury, Adaptation and Death
Harvard-MIT Division of Health Sciences and Technology HST.035: Principle and Practice of Human Pathology Dr. Badizadegan Cell Injury, Adaptation and Death HST.035 Spring 2003 Overview of Cell Injury Cells
More informationAP BIOLOGY CHAPTER 7 Cellular Respiration Outline
AP BIOLOGY CHAPTER 7 Cellular Respiration Outline I. How cells get energy. A. Cellular Respiration 1. Cellular respiration includes the various metabolic pathways that break down carbohydrates and other
More informationAn Overview of Cells and Cell Research
An Overview of Cells and Cell Research 1 An Overview of Cells and Cell Research Chapter Outline Model Species and Cell types Cell components Tools of Cell Biology Model Species E. Coli: simplest organism
More informationab185915 Protein Sumoylation Assay Ultra Kit
ab185915 Protein Sumoylation Assay Ultra Kit Instructions for Use For the measuring in vivo protein sumoylation in various samples This product is for research use only and is not intended for diagnostic
More informationClassical drug resistance in the molecular age
Classical drug resistance in the molecular age Robert O Connor, Ph.D. Senior Programme Leader, Translational Cancer Pharmacology Lecturer in Biological Sciences, School of Nursing Chair ICORG Translational
More informationMedical Therapies Limited EGM Presentation
Medical Therapies Limited EGM Presentation Maria Halasz Chief Executive Officer 5 May 2009 1 Agenda 1. Company information 2. Recent developments 3. Business strategy 4. Key value inflection points for
More informationGraduate and Postdoctoral Affairs School of Biomedical Sciences College of Medicine. Graduate Certificate. Metabolic & Nutritional Medicine
Graduate and Postdoctoral Affairs School of Biomedical Sciences College of Medicine Graduate Certificate in Metabolic & Nutritional Medicine Graduate Certificate Metabolic & Nutritional Medicine Purpose
More informationIntroduction to Enteris BioPharma
Introduction to Enteris BioPharma Enteris BioPharma Intelligent Solutions for Oral Drug Delivery Privately held, New Jersey based biotech company Owned solely by Victory Park Capital, a large Chicago based
More informationPredict Reactivity Note Chicken (100%), Sheep (100%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (100%), Guinea pig (100%)
Datasheet GeneTex, Inc : Toll Free 1-877-GeneTex (1-877-436-3839) Fax:1-949-309-2888 info@genetex.com GeneTex International Corporation : Tel:886-3-6208988 Fax:886-3-6208989 infoasia@genetex.com Date :
More informationObjectives. Immunologic Methods. Objectives. Immunology vs. Serology. Cross Reactivity. Sensitivity and Specificity. Definitions
Immunologic Methods Part One Definitions Part Two Antigen-Antibody Reactions CLS 420 Clinical Immunology and Molecular Diagnostics Kathy Trudell MLS (ASCP) CM SBB CM ktrudell@nebraskamed.com Discuss the
More informationAccelerated Stability During Formulation Development of Early Stage Protein Therapeutics Pros and Cons of Contrasting Approaches
Accelerated Stability During Formulation Development of Early Stage Protein Therapeutics Pros and Cons of Contrasting Approaches 2008 IBC Formulation Strategies for Protein Therapeutics Tim Kelly, Ph.D.
More informationCell Membrane Structure (and How to Get Through One)
Cell Membrane Structure (and How to Get Through One) A cell s membrane is a wall of sorts that defines the boundaries of a cell. The membrane provides protection and structure for the cell and acts as
More informationThe diagram below summarizes the effects of the compounds that cells use to regulate their own metabolism.
Regulation of carbohydrate metabolism Intracellular metabolic regulators Each of the control point steps in the carbohydrate metabolic pathways in effect regulates itself by responding to molecules that
More informationPaclitaxel-Loaded Expansile Nanoparticles Enhance Chemotheraputic Drug-delivery in Mesothelioma 3D Multicellular Spheroids
Paclitaxel-Loaded Expansile Nanoparticles Enhance Chemotheraputic Drug-delivery in Mesothelioma 3D Multicellular Spheroids Hongyi Lei 1, Rong Liu 1, Aaron Colby 2, Mark W. Grinstaff 2, Yolonda L. Colson
More informationCHAPTER 2 ANTIGEN/ANTIBODY INTERACTIONS
CHAPTER 2 ANTIGEN/ANTIBODY INTERACTIONS See APPENDIX (1) THE PRECIPITIN CURVE; (2) LABELING OF ANTIBODIES The defining characteristic of HUMORAL immune responses (which distinguishes them from CELL-MEDIATED
More information3D Cell Culture mimsys G
3D Cell Culture mimsys G xeno-free & nutrient permeable hydrogel for 3D cell culture mimsys G is a xeno-free and non-immunogenic, easy to handle hydrogel for cell encapsulation in 3D experiments in vitro
More informationCell Transport and Plasma Membrane Structure
Cell Transport and Plasma Membrane Structure POGIL Guided Inquiry Learning Targets Explain the importance of the plasma membrane. Compare and contrast different types of passive transport. Explain how
More informationUses of Flow Cytometry
Uses of Flow Cytometry 1. Multicolour analysis... 2 2. Cell Cycle and Proliferation... 3 a. Analysis of Cellular DNA Content... 4 b. Cell Proliferation Assays... 5 3. Immunology... 6 4. Apoptosis... 7
More informationUnderstanding ph management and plant nutrition Part 5: Choosing the best fertilizer
Understanding ph management and plant nutrition Part 5: Choosing the best fertilizer Bill Argo, Ph.D. Blackmore Company, Tel: 800-874-8660, Int l 734-483-8661, E-mail: bargo@blackmoreco.com Originally
More informationRayBio Creatine Kinase (CK) Activity Colorimetric Assay Kit
RayBio Creatine Kinase (CK) Activity Colorimetric Assay Kit User Manual Version 1.0 May 28, 2014 RayBio Creatine Kinase Activity Colorimetric Assay (Cat#: 68CL-CK-S100) RayBiotech, Inc. We Provide You
More informationSeahorse XF Cell Mito Stress Test Kit
Seahorse XF Cell Mito Stress Test Kit Part # 103015-100 User Guide For use with Seahorse XF e and XF Extracellular Flux Analyzers For Research Use Only 1 Table of Contents Product Description... 2 Introduction...
More information博 士 論 文 ( 要 約 ) A study on enzymatic synthesis of. stable cyclized peptides which. inhibit protein-protein interactions
博 士 論 文 ( 要 約 ) 論 文 題 目 A study on enzymatic synthesis of stable cyclized peptides which inhibit protein-protein interactions ( 蛋 白 質 間 相 互 作 用 を 阻 害 する 安 定 な 環 状 化 ペプチドの 酵 素 合 成 に 関 する 研 究 ) 氏 名 張 静 1
More informationComparison of Trypan Blue and Fluorescence-Based Viability Detection Methods Via Morphological Observation
Comparison of Trypan Blue and Fluorescence-Based Viability Detection Methods Via Morphological Observation Nexcelom Bioscience LLC. 360 Merrimack Street, Building 9 Lawrence, MA 01843 T: 978.327.5340 F:
More informationHyStem. Hydrogels CELLULAR MATRICES FOR TRANSLATIONAL RESEARCH. esibio.com
HyStem Hydrogels CELLULAR MATRICES FOR TRANSLATIONAL RESEARCH HyStem Hydrogel Extracellular Matrices Chemically-defined For use in 2D and 3D formats in vitro and in vivo applications Customizable for many
More information7 Answers to end-of-chapter questions
7 Answers to end-of-chapter questions Multiple choice questions 1 B 2 B 3 A 4 B 5 A 6 D 7 C 8 C 9 B 10 B Structured questions 11 a i Maintenance of a constant internal environment within set limits i Concentration
More informationIntroduction to Flow Cytometry
Outline Introduction to Flow Cytometry Basic Concept of Flow Cytometry Introduction to Instrument Subsystems Daisy Kuo Assistant Product Manager E-mail: daisy_kuo@bd.com BDBiosciences Application Examples
More informationChapter 7 Active Reading Guide Cellular Respiration and Fermentation
Name: AP Biology Mr. Croft Chapter 7 Active Reading Guide Cellular Respiration and Fermentation Overview: Before getting involved with the details of cellular respiration and photosynthesis, take a second
More informationArC Amine Reactive Compensation Bead Kit
ArC Amine Reactive Compensation Bead Kit Catalog no. A1346 Table 1. Contents and storage information. Material Amount Composition Storage Stability ArC reactive beads (Component A) ArC negative beads (Component
More informationCytoSelect Cell Viability and Cytotoxicity Assay Kit
Product Manual CytoSelect Cell Viability and Cytotoxicity Assay Kit Catalog Number CBA-240 96 assays (96-well plate) FOR RESEARCH USE ONLY Not for use in diagnostic procedures Introduction The measurement
More informationCancer SBL101. James Gomes School of Biological Sciences Indian Institute of Technology Delhi
Cancer SBL101 James Gomes School of Biological Sciences Indian Institute of Technology Delhi All Figures in this Lecture are taken from 1. Molecular biology of the cell / Bruce Alberts et al., 5th ed.
More informationIntravenous Fluid Selection
BENNMC03_0131186116.qxd 3/9/05 18:24 Page 20 seema Seema-3:Desktop Folder:PQ731: CHAPTER 3 Intravenous Fluid Selection LEARNING OBJECTIVES By the end of this chapter, you should be able to: Describe and
More informationXinnan Wang Stanford University School of Medicine
Xinnan Wang Stanford University School of Medicine Cellular Organelles are Highly Dynamic COS7 Cell, MitoTracker Fission-fusion, changing morphology and locabon To meet ever-changing energy demand Electrical
More informationWeek 30. Water Balance and Minerals
Week 30 Water Balance and Minerals Water: more vital to life than food involved in almost every body function is not stored--excreted daily largest single constituent of the human body, averaging 60% of
More informationWilliam Shaw, Ph.D. The Great Plains Laboratory, Inc., Lenexa, Kansas, USA
Inhibition of dopamine conversion to norepinephrine by Clostridia metabolites appears to be a (the) major cause of autism, schizophrenia, and other neuropsychiatric disorders. All these factors can now
More informationStem Cell-based Therapies and FDA Regulations
Stem Cell-based Therapies and FDA Regulations By Trina Slabiak, MS, RAC Stem cell-based therapies are arriving in the clinic as Phase 1 studies. Which technological challenges and US Food and Drug Administration
More informationAP BIOLOGY 2008 SCORING GUIDELINES
AP BIOLOGY 2008 SCORING GUIDELINES Question 1 1. The physical structure of a protein often reflects and affects its function. (a) Describe THREE types of chemical bonds/interactions found in proteins.
More informationSafe Nano Design Molecule Manufacturing Market
August 14 16, 212 College of Nanoscale Science & Engineering (CNSE) of the University at Albany National Institute for Occupational Safety and Health (NIOSH) Prevention through Design Program Safe Nano
More informationNon Specific Binding (NSB) in Antigen-Antibody Assays
Non Specific Binding (NSB) in Antigen-Antibody Assays Chem 395 Spring 2007 Instructor : Dr. James Rusling Presenter : Bhaskara V. Chikkaveeraiah OUTLINE Immunoassays Introduction Factors contributing to
More informationVersion 1 2015. Module guide. Preliminary document. International Master Program Cardiovascular Science University of Göttingen
Version 1 2015 Module guide International Master Program Cardiovascular Science University of Göttingen Part 1 Theoretical modules Synopsis The Master program Cardiovascular Science contains four theoretical
More informationFuture Oncology: Technology, Products, Market and Service Opportunities
Brochure More information from http://www.researchandmarkets.com/reports/296370/ Future Oncology: Technology, Products, Market and Service Opportunities Description: Future Oncology is an analytical newsletter
More informationPART I: Neurons and the Nerve Impulse
PART I: Neurons and the Nerve Impulse Identify each of the labeled structures of the neuron below. A. B. C. D. E. F. G. Identify each of the labeled structures of the neuron below. A. dendrites B. nucleus
More information