Transmembrane proteins span the bilayer. α-helix transmembrane domain. Multiple transmembrane helices in one polypeptide

Size: px
Start display at page:

Download "Transmembrane proteins span the bilayer. α-helix transmembrane domain. Multiple transmembrane helices in one polypeptide"

Transcription

1 Transmembrane proteins span the bilayer α-helix transmembrane domain Hydrophobic R groups of a.a. interact with fatty acid chains Multiple transmembrane helices in one polypeptide Polar a.a. Hydrophilic pore Nonpolar a.a. Membrane transporter for polar or charged molecules ECB Fig Mobility of transmembrane proteins Bleach with laser beam If protein is mobile then fluorescent signal moves back into bleached area Recovery rate measures mobility 2

2 Peripheral membrane proteins (associated with membrane, but not in bilayer) Lecture 5 (cont d) Membrane Proteins Proteins as enzymes Binding sites Free energy Activation energy, enzyme function Enzyme mechanisms Kinetic parameters of enzymes Proteins as membrane transporters Enzymes bind substrates Substrate (ligand) Non-covalent interactions Binding site ECB Fig Enzyme (protein) 3

3 How do enzymes work? Start by considering free energy Free energy is amount of useful energy available to do work G (Delta G) = free energy change (Reactants - Products) In a chemical reaction G = H T S H = heat; heat released is negative S = entropy (randomness); increased randomness is positive Reactions occur spontaneously if G is negative Enzymes lower activation energy but have NO effect on G Energy of reactants Activation energy G Energy of products ECB Fig Uncatalyzed reaction Catalyzed reaction Enzymes accelerate reaction rates X Y Uncatalyzed reaction X Y Enzyme catalyzed reaction ECB Fig

4 How do enzymes accelerate reactions? Enzymes can hold substrates in positions that encourage reactions to occur Enzymes can change the ionic environment of substrates, accelerating the reaction Lower activation energy Enzymes can put physical stress on substrates Adapted from ECB Fig Thermodynamically Unfavorable Reactions ( G+) Many reactions in cells have positive G: e.g. condensation reactions (forming polymers reduces randomness so S -, G +) G = H T S Y G + Solution: couple to reaction where G - (Often hydrolysis of ATP) X Y G + ATP ADP + P i G - X + ATP Y + ADP + Pi + G - Example of coupled reaction: synthesis of sucrose ECB Panel 3-1 G values are additive 5

5 ATP (Nucleotide) G of hydrolysis = -7.3 kcal/mole ADP + P i + energy Enzymes can be regulated Inhibitors can bind to active site Binding in the active site can prevent substrate interaction Enzymes can be regulated at sites other than the active site Example: phosphorylation Fig ECB

6 Lecture 5 Outline Protein Secondary Structure Membrane Proteins Proteins as enzymes Proteins as membrane transporters (Ch 12 ECB) Channel Carrier proteins Facilitated diffusion Active transport Lipid Bilayer Permeability Small hydrophobic Molecules O 2, CO 2, N 2, benzene Properties of a pure synthetic lipid bilayer Small Uncharged polar molecules H 2 O, glycerol, ethanol Large, uncharged Polar molecules Amino acids, glucose, nucleotides IONS H +, Na +, HCO 3 -, K +, Ca 2+, Cl -, Mg 2+ ECB 12-2 Transmembrane proteins allow movement of molecules that cannot move through bilayer ECB 12-1 But it is not that simple 7

7 Membrane impermeability results in electrical and chemical gradients across membrane Charged molecules - transport influenced by concentration gradient and membrane potential (electrochemical (EC) gradient ) out Electrochemical gradient in ECB 12-8 Concentration gradient only Conc.. Gradient with membrane potential (-) inside Ion gradients across the plasma membrane ph 7.2* ph 7.4* Different electrochemical gradient for each ion Electrical and concentration gradient can be opposite (e.g. K + ) Transport problems faced by cells: - Need to get an impermeable molecule across the membrane - going WITH its electrochemical gradient - Need to get a molecule (permeable or impermeable) across the membrane going AGAINST its electrochemical gradient Solution -- specialized membrane proteins for transport functions. 8

8 Two broad classes of transmembrane proteins A. channel protein B. carrier proteins ECB 12-3 Conformational change Transport can be passive or active electrochemical ECB 12-4 Channels - Passive transport down elecrochemical gradient Impermeable Channel protein ECB 12-4 Channel-mediated diffusion (facilitated diffusion) 9

9 Channel structure Aqueous pore due to polar and charged R groups ECB Always passive transport Mechanism of K + channel selectivity ECB 12-7 Carrier mediated Diffusion (facilitated diffusion down EC gradient) Active transport (energy-driven) Carrier Proteins: Transport against EC gradient Transfer across membrane driven by conformational change in transporter Slower than channels Binds transported ligand - highly specific 10

10 Active transport - three types -uses energy to drive transport against EC gradient through carrier protein ECB 12-9 Coupled transport Down EC gradient Cotransported Molecule (against EC gradient) ECB Symport- - move same direction Antiport- - move opposite directions Na-Glucose symporter Move glucose against its EC gradient, using the energy stored in the Na + gradient. ECB

11 ATP-driven pumps Move against EC gradient ATP ADP + Pi Typically move ions generating EC gradient EC gradient can then be used in coupled transport Na + /K + pump in animal cells ECB Cyclic transport by Na + /K + pump Phosphoryation regulates the enzyme conformation 3 Conf. change 1 Low affinity Na binding sites High affinity K binding sites 3 High affinity Na binding sites Low affinity K+ binding sites 3 2 NaKATPase.avi 2 Conf. change

12 Chemiosmotic coupling of pumps and cotransport H + transporters in vacuole and lysosome are similar Osmosis Osmosis: movement of water from region of low solute concentration to region of high solute concentration (or high water potential to low water potential) How do cells prevent osmotic swelling? ECB

Total body water ~(60% of body mass): Intracellular fluid ~2/3 or ~65% Extracellular fluid ~1/3 or ~35% fluid. Interstitial.

Total body water ~(60% of body mass): Intracellular fluid ~2/3 or ~65% Extracellular fluid ~1/3 or ~35% fluid. Interstitial. http://www.bristol.ac.uk/phys-pharm/teaching/staffteaching/sergeykasparov.htmlpharm/teaching/staffteaching/sergeykasparov.html Physiology of the Cell Membrane Membrane proteins and their roles (channels,

More information

Modes of Membrane Transport

Modes of Membrane Transport Modes of Membrane Transport Transmembrane Transport movement of small substances through a cellular membrane (plasma, ER, mitochondrial..) ions, fatty acids, H 2 O, monosaccharides, steroids, amino acids

More information

BIOLOGICAL MEMBRANES: FUNCTIONS, STRUCTURES & TRANSPORT

BIOLOGICAL MEMBRANES: FUNCTIONS, STRUCTURES & TRANSPORT BIOLOGICAL MEMBRANES: FUNCTIONS, STRUCTURES & TRANSPORT UNIVERSITY OF PNG SCHOOL OF MEDICINE AND HEALTH SCIENCES DISCIPLINE OF BIOCHEMISTRY AND MOLECULAR BIOLOGY BMLS II / B Pharm II / BDS II VJ Temple

More information

The Lipid Bilayer Is a Two-Dimensional Fluid

The Lipid Bilayer Is a Two-Dimensional Fluid The Lipid Bilayer Is a Two-Dimensional Fluid The aqueous environment inside and outside a cell prevents membrane lipids from escaping from bilayer, but nothing stops these molecules from moving about and

More information

Cell Biology - Part 2 Membranes

Cell Biology - Part 2 Membranes Cell Biology - Part 2 Membranes The organization of cells is made possible by membranes. Membranes isolate, partition, and compartmentalize cells. 1 Membranes isolate the inside of the cell from the outside

More information

Membrane Structure and Function

Membrane Structure and Function Membrane Structure and Function -plasma membrane acts as a barrier between cells and the surrounding. -plasma membrane is selective permeable -consist of lipids, proteins and carbohydrates -major lipids

More information

Six major functions of membrane proteins: Transport Enzymatic activity

Six major functions of membrane proteins: Transport Enzymatic activity CH 7 Membranes Cellular Membranes Phospholipids are the most abundant lipid in the plasma membrane. Phospholipids are amphipathic molecules, containing hydrophobic and hydrophilic regions. The fluid mosaic

More information

Student name ID # 2. (4 pts) What is the terminal electron acceptor in respiration? In photosynthesis? O2, NADP+

Student name ID # 2. (4 pts) What is the terminal electron acceptor in respiration? In photosynthesis? O2, NADP+ 1. Membrane transport. A. (4 pts) What ion couples primary and secondary active transport in animal cells? What ion serves the same function in plant cells? Na+, H+ 2. (4 pts) What is the terminal electron

More information

1.1.2. thebiotutor. AS Biology OCR. Unit F211: Cells, Exchange & Transport. Module 1.2 Cell Membranes. Notes & Questions.

1.1.2. thebiotutor. AS Biology OCR. Unit F211: Cells, Exchange & Transport. Module 1.2 Cell Membranes. Notes & Questions. thebiotutor AS Biology OCR Unit F211: Cells, Exchange & Transport Module 1.2 Cell Membranes Notes & Questions Andy Todd 1 Outline the roles of membranes within cells and at the surface of cells. The main

More information

CELL MEMBRANES, TRANSPORT, and COMMUNICATION. Teacher Packet

CELL MEMBRANES, TRANSPORT, and COMMUNICATION. Teacher Packet AP * BIOLOGY CELL MEMBRANES, TRANSPORT, and COMMUNICATION Teacher Packet AP* is a trademark of the College Entrance Examination Board. The College Entrance Examination Board was not involved in the production

More information

CHAPTER 5.1 5.2: Plasma Membrane Structure

CHAPTER 5.1 5.2: Plasma Membrane Structure CHAPTER 5.1 5.2: Plasma Membrane Structure 1. Describe the structure of a phospholipid molecule. Be sure to describe their behavior in relationship to water. 2. What happens when a collection of phospholipids

More information

Anatomy and Physiology Placement Exam 2 Practice with Answers at End!

Anatomy and Physiology Placement Exam 2 Practice with Answers at End! Anatomy and Physiology Placement Exam 2 Practice with Answers at End! General Chemical Principles 1. bonds are characterized by the sharing of electrons between the participating atoms. a. hydrogen b.

More information

PART I: Neurons and the Nerve Impulse

PART I: Neurons and the Nerve Impulse PART I: Neurons and the Nerve Impulse Identify each of the labeled structures of the neuron below. A. B. C. D. E. F. G. Identify each of the labeled structures of the neuron below. A. dendrites B. nucleus

More information

Cell Membrane Coloring Worksheet

Cell Membrane Coloring Worksheet Cell Membrane Coloring Worksheet Composition of the Cell Membrane & Functions The cell membrane is also called the plasma membrane and is made of a phospholipid bilayer. The phospholipids have a hydrophilic

More information

Todays Outline. Metabolism. Why do cells need energy? How do cells acquire energy? Metabolism. Concepts & Processes. The cells capacity to:

Todays Outline. Metabolism. Why do cells need energy? How do cells acquire energy? Metabolism. Concepts & Processes. The cells capacity to: and Work Metabolic Pathways Enzymes Features Factors Affecting Enzyme Activity Membrane Transport Diffusion Osmosis Passive Transport Active Transport Bulk Transport Todays Outline -Releasing Pathways

More information

Ch. 8 - The Cell Membrane

Ch. 8 - The Cell Membrane Ch. 8 - The Cell Membrane 2007-2008 Phospholipids Phosphate head hydrophilic Fatty acid tails hydrophobic Arranged as a bilayer Phosphate attracted to water Fatty acid repelled by water Aaaah, one of those

More information

NO CALCULATORS OR CELL PHONES ALLOWED

NO CALCULATORS OR CELL PHONES ALLOWED Biol 205 Exam 1 TEST FORM A Spring 2008 NAME Fill out both sides of the Scantron Sheet. On Side 2 be sure to indicate that you have TEST FORM A The answers to Part I should be placed on the SCANTRON SHEET.

More information

Cell Membrane Structure (and How to Get Through One)

Cell Membrane Structure (and How to Get Through One) Cell Membrane Structure (and How to Get Through One) A cell s membrane is a wall of sorts that defines the boundaries of a cell. The membrane provides protection and structure for the cell and acts as

More information

Ions cannot cross membranes. Ions move through pores

Ions cannot cross membranes. Ions move through pores Ions cannot cross membranes Membranes are lipid bilayers Nonpolar tails Polar head Fig 3-1 Because of the charged nature of ions, they cannot cross a lipid bilayer. The ion and its cloud of polarized water

More information

Cell Membrane & Tonicity Worksheet

Cell Membrane & Tonicity Worksheet NAME ANSWER KEY DATE PERIOD Cell Membrane & Tonicity Worksheet Composition of the Cell Membrane & Functions The cell membrane is also called the PLASMA membrane and is made of a phospholipid BI-LAYER.

More information

Chapter 8. Movement across the Cell Membrane. AP Biology

Chapter 8. Movement across the Cell Membrane. AP Biology Chapter 8. Movement across the Cell Membrane More than just a barrier Expanding our view of cell membrane beyond just a phospholipid bilayer barrier phospholipids plus Fluid Mosaic Model In 1972, S.J.

More information

Chemical Basis of Life Module A Anchor 2

Chemical Basis of Life Module A Anchor 2 Chemical Basis of Life Module A Anchor 2 Key Concepts: - Water is a polar molecule. Therefore, it is able to form multiple hydrogen bonds, which account for many of its special properties. - Water s polarity

More information

Mammalian Physiology. Cellular Membranes Membrane Transport UNLV. PHYSIOLOGY, Chapter 1 Berne, Levy, Koeppen, Stanton UNIVERSITY OF NEVADA LAS VEGAS

Mammalian Physiology. Cellular Membranes Membrane Transport UNLV. PHYSIOLOGY, Chapter 1 Berne, Levy, Koeppen, Stanton UNIVERSITY OF NEVADA LAS VEGAS Mammalian Physiology Cellular Membranes Membrane Transport UNLV 1 UNIVERSITY OF NEVADA LAS VEGAS PHYSIOLOGY, Chapter 1 Berne, Levy, Koeppen, Stanton Objectives Describe the structure of the cell membrane

More information

BSC 2010 - Exam I Lectures and Text Pages. The Plasma Membrane Structure and Function. Phospholipids. I. Intro to Biology (2-29) II.

BSC 2010 - Exam I Lectures and Text Pages. The Plasma Membrane Structure and Function. Phospholipids. I. Intro to Biology (2-29) II. BSC 2010 - Exam I Lectures and Text Pages I. Intro to Biology (2-29) II. Chemistry of Life Chemistry review (30-46) Water (47-57) Carbon (58-67) Macromolecules (68-91) III. Cells and Membranes Cell structure

More information

Copyright The McGraw-Hill Companies, Inc. Permission required for reproduction or display. Two Forms of Energy

Copyright The McGraw-Hill Companies, Inc. Permission required for reproduction or display. Two Forms of Energy Module 2D - Energy and Metabolism Objective # 19 All living organisms require energy for survival. In this module we will examine some general principles about chemical reactions and energy usage within

More information

FIGURE 2.18. A. The phosphate end of the molecule is polar (charged) and hydrophilic (attracted to water).

FIGURE 2.18. A. The phosphate end of the molecule is polar (charged) and hydrophilic (attracted to water). PLASMA MEMBRANE 1. The plasma membrane is the outermost part of a cell. 2. The main component of the plasma membrane is phospholipids. FIGURE 2.18 A. The phosphate end of the molecule is polar (charged)

More information

Unit 2: Cells, Membranes and Signaling CELL MEMBRANE. Chapter 5 Hillis Textbook

Unit 2: Cells, Membranes and Signaling CELL MEMBRANE. Chapter 5 Hillis Textbook Unit 2: Cells, Membranes and Signaling CELL MEMBRANE Chapter 5 Hillis Textbook HOW DOES THE LAB RELATE TO THE NEXT CHAPTER? SURFACE AREA: the entire outer covering of a cell that enables materials pass.

More information

Keystone Review Practice Test Module A Cells and Cell Processes. 1. Which characteristic is shared by all prokaryotes and eukaryotes?

Keystone Review Practice Test Module A Cells and Cell Processes. 1. Which characteristic is shared by all prokaryotes and eukaryotes? Keystone Review Practice Test Module A Cells and Cell Processes 1. Which characteristic is shared by all prokaryotes and eukaryotes? a. Ability to store hereditary information b. Use of organelles to control

More information

Biological cell membranes

Biological cell membranes Unit 14: Cell biology. 14 2 Biological cell membranes The cell surface membrane surrounds the cell and acts as a barrier between the cell s contents and the environment. The cell membrane has multiple

More information

Cells and Their Housekeeping Functions Cell Membrane & Membrane Potential

Cells and Their Housekeeping Functions Cell Membrane & Membrane Potential Cells and Their Housekeeping Functions Cell Membrane & Membrane Potential Shu-Ping Lin, Ph.D. Institute of Biomedical Engineering E-mail: splin@dragon.nchu.edu.tw Website: http://web.nchu.edu.tw/pweb/users/splin/

More information

Carbohydrates, proteins and lipids

Carbohydrates, proteins and lipids Carbohydrates, proteins and lipids Chapter 3 MACROMOLECULES Macromolecules: polymers with molecular weights >1,000 Functional groups THE FOUR MACROMOLECULES IN LIFE Molecules in living organisms: proteins,

More information

The correct answer is d C. Answer c is incorrect. Reliance on the energy produced by others is a characteristic of heterotrophs.

The correct answer is d C. Answer c is incorrect. Reliance on the energy produced by others is a characteristic of heterotrophs. 1. An autotroph is an organism that a. extracts energy from organic sources b. converts energy from sunlight into chemical energy c. relies on the energy produced by other organisms as an energy source

More information

BCOR 011 Exam 2, 2004

BCOR 011 Exam 2, 2004 BCOR 011 Exam 2, 2004 Name: Section: MULTIPLE CHOICE. Choose the one alternative that best completes the statement or answers the question. 1. According to the first law of thermodynamics, A. the universe

More information

MULTIPLE CHOICE QUESTIONS

MULTIPLE CHOICE QUESTIONS MULTIPLE CHOICE QUESTIONS 1. Most components of energy conversion systems evolved very early; thus, the most fundamental aspects of energy metabolism tend to be: A. quite different among a diverse group

More information

Biological Membranes. Impermeable lipid bilayer membrane. Protein Channels and Pores

Biological Membranes. Impermeable lipid bilayer membrane. Protein Channels and Pores Biological Membranes Impermeable lipid bilayer membrane Protein Channels and Pores 1 Biological Membranes Are Barriers for Ions and Large Polar Molecules The Cell. A Molecular Approach. G.M. Cooper, R.E.

More information

4. Biology of the Cell

4. Biology of the Cell 4. Biology of the Cell Our primary focus in this chapter will be the plasma membrane and movement of materials across the plasma membrane. You should already be familiar with the basic structures and roles

More information

Membrane Transport. Extracellular Concentration of X

Membrane Transport. Extracellular Concentration of X Use the following graph to answer questions 1 and 2. Rate of diffusion of X into the cell 1. Which of the following processes is represented by the above graph? c. Active transport 2. Molecule X is most

More information

Chapter 8: An Introduction to Metabolism

Chapter 8: An Introduction to Metabolism Chapter 8: An Introduction to Metabolism Name Period Concept 8.1 An organism s metabolism transforms matter and energy, subject to the laws of thermodynamics 1. Define metabolism. The totality of an organism

More information

Chapter 8: Energy and Metabolism

Chapter 8: Energy and Metabolism Chapter 8: Energy and Metabolism 1. Discuss energy conversions and the 1 st and 2 nd law of thermodynamics. Be sure to use the terms work, potential energy, kinetic energy, and entropy. 2. What are Joules

More information

Ordered Structures of Lipids - Bilayers form spontaneously over large areas

Ordered Structures of Lipids - Bilayers form spontaneously over large areas Membranes What are the purposes of membranes? Physical barriers/compartmentalization Gatekeepers exclusion of toxic molecules Energy and signal transduction Aid in cell locomotion Cell-cell interactions

More information

8/20/2012 H C OH H R. Proteins

8/20/2012 H C OH H R. Proteins Proteins Rubisco monomer = amino acids 20 different amino acids polymer = polypeptide protein can be one or more polypeptide chains folded & bonded together large & complex 3-D shape hemoglobin Amino acids

More information

Membrane Structure, Transport, and Cell Junctions

Membrane Structure, Transport, and Cell Junctions Membrane Structure, Transport, and Cell Junctions 5 A model for the structure of aquaporin. This protein, found in the plasma membrane of many cell types, such as red blood cells and plant cells, forms

More information

AP BIOLOGY CHAPTER 7 Cellular Respiration Outline

AP BIOLOGY CHAPTER 7 Cellular Respiration Outline AP BIOLOGY CHAPTER 7 Cellular Respiration Outline I. How cells get energy. A. Cellular Respiration 1. Cellular respiration includes the various metabolic pathways that break down carbohydrates and other

More information

Lecture 15: Enzymes & Kinetics Mechanisms

Lecture 15: Enzymes & Kinetics Mechanisms ROLE OF THE TRANSITION STATE Lecture 15: Enzymes & Kinetics Mechanisms Consider the reaction: H-O-H + Cl - H-O δ- H Cl δ- HO - + H-Cl Reactants Transition state Products Margaret A. Daugherty Fall 2004

More information

Electron Transport and Oxidative Phosphorylation

Electron Transport and Oxidative Phosphorylation CHM333 LECTURES 37 & 38: 4/27 29/13 SPRING 2013 Professor Christine Hrycyna Electron Transport and Oxidative Phosphorylation Final stages of aerobic oxidation of biomolecules in eukaryotes occur in the

More information

Bioenergetics. Free Energy Change

Bioenergetics. Free Energy Change Bioenergetics Energy is the capacity or ability to do work All organisms need a constant supply of energy for functions such as motion, transport across membrane barriers, synthesis of biomolecules, information

More information

2007 7.013 Problem Set 1 KEY

2007 7.013 Problem Set 1 KEY 2007 7.013 Problem Set 1 KEY Due before 5 PM on FRIDAY, February 16, 2007. Turn answers in to the box outside of 68-120. PLEASE WRITE YOUR ANSWERS ON THIS PRINTOUT. 1. Where in a eukaryotic cell do you

More information

IB104 - Lecture 9 - Membranes

IB104 - Lecture 9 - Membranes There have been many magnificent boats built to try to reach 50 knots. This was the creation of an Australian team that held the record for more than a decade, from 1993 till 2005, at 46.5 knots with their

More information

Date: Student Name: Teacher Name: Jared George. Score: 1) A cell with 1% solute concentration is placed in a beaker with a 5% solute concentration.

Date: Student Name: Teacher Name: Jared George. Score: 1) A cell with 1% solute concentration is placed in a beaker with a 5% solute concentration. Biology Keystone (PA Core) Quiz Homeostasis and Transport - (BIO.A.4.1.1 ) Plasma Membrane, (BIO.A.4.1.2 ) Transport Mechanisms, (BIO.A.4.1.3 ) Transport Facilitation Student Name: Teacher Name: Jared

More information

Energy & Enzymes. Life requires energy for maintenance of order, growth, and reproduction. The energy living things use is chemical energy.

Energy & Enzymes. Life requires energy for maintenance of order, growth, and reproduction. The energy living things use is chemical energy. Energy & Enzymes Life requires energy for maintenance of order, growth, and reproduction. The energy living things use is chemical energy. 1 Energy exists in two forms - potential and kinetic. Potential

More information

Cellular Energy: ATP & Enzymes. What is it? Where do organism s get it? How do they use it?

Cellular Energy: ATP & Enzymes. What is it? Where do organism s get it? How do they use it? Cellular Energy: ATP & Enzymes What is it? Where do organism s get it? How do they use it? Where does Energy come from? Ultimately, from the sun. It is transferred between organisms in the earth s lithosphere,

More information

Chapter 7: Membrane Structure and Function

Chapter 7: Membrane Structure and Function Name Period Concept 7.1 Cellular membranes are fluid mosaics of lipids and proteins 1. The large molecules of all living things fall into just four main classes. Name them. 2. Explain what is meant when

More information

Proteins and Nucleic Acids

Proteins and Nucleic Acids Proteins and Nucleic Acids Chapter 5 Macromolecules: Proteins Proteins Most structurally & functionally diverse group of biomolecules. : o Involved in almost everything o Enzymes o Structure (keratin,

More information

Electron Transport Generates a Proton Gradient Across the Membrane

Electron Transport Generates a Proton Gradient Across the Membrane Electron Transport Generates a Proton Gradient Across the Membrane Each of respiratory enzyme complexes couples the energy released by electron transfer across it to an uptake of protons from water in

More information

CELL MEMBRANE & CELL TRANSPORT (PASSIVE and ACTIVE) Webquest

CELL MEMBRANE & CELL TRANSPORT (PASSIVE and ACTIVE) Webquest Name: Period: CELL MEMBRANE & CELL TRANSPORT (PASSIVE and ACTIVE) Webquest PART I: CELL MEMBRANES WEBSITE #1: http://www.wisc-online.com/objects/index_tj.asp?objid=ap1101 1. What is the BASIC UNIT of LIFE?

More information

Chapter 3. Protein Structure and Function

Chapter 3. Protein Structure and Function Chapter 3 Protein Structure and Function Broad functional classes So Proteins have structure and function... Fine! -Why do we care to know more???? Understanding functional architechture gives us POWER

More information

Chapter 5: The Structure and Function of Large Biological Molecules

Chapter 5: The Structure and Function of Large Biological Molecules Name Period Concept 5.1 Macromolecules are polymers, built from monomers 1. The large molecules of all living things fall into just four main classes. Name them. 2. Circle the three classes that are called

More information

Chapter 19a Oxidative Phosphorylation and Photophosphorylation. Multiple Choice Questions

Chapter 19a Oxidative Phosphorylation and Photophosphorylation. Multiple Choice Questions Chapter 19a Oxidative Phosphorylation and Photophosphorylation Multiple Choice Questions 1. Electron-transfer reactions in mitochondria Page: 707 Difficulty: 1 Ans: E Almost all of the oxygen (O 2 ) one

More information

Chem 306 Chapter 21 Bioenergetics Lecture Outline III

Chem 306 Chapter 21 Bioenergetics Lecture Outline III Chem 306 Chapter 21 Bioenergetics Lecture Outline III I. HOW IS ATP GENERATED IN THE FINAL STAGE CATABOLISM? A. OVERVIEW 1. At the end of the citric acid cycle, all six carbons of glucose have been oxidized

More information

AP Bio Photosynthesis & Respiration

AP Bio Photosynthesis & Respiration AP Bio Photosynthesis & Respiration Multiple Choice Identify the letter of the choice that best completes the statement or answers the question. 1. What is the term used for the metabolic pathway in which

More information

AP BIOLOGY 2008 SCORING GUIDELINES

AP BIOLOGY 2008 SCORING GUIDELINES AP BIOLOGY 2008 SCORING GUIDELINES Question 1 1. The physical structure of a protein often reflects and affects its function. (a) Describe THREE types of chemical bonds/interactions found in proteins.

More information

THE HISTORY OF CELL BIOLOGY

THE HISTORY OF CELL BIOLOGY SECTION 4-1 REVIEW THE HISTORY OF CELL BIOLOGY Define the following terms. 1. cell 2. cell theory Write the correct letter in the blank. 1. One early piece of evidence supporting the cell theory was the

More information

What affects an enzyme s activity? General environmental factors, such as temperature and ph. Chemicals that specifically influence the enzyme.

What affects an enzyme s activity? General environmental factors, such as temperature and ph. Chemicals that specifically influence the enzyme. CH s 8-9 Respiration & Metabolism Metabolism A catalyst is a chemical agent that speeds up a reaction without being consumed by the reaction. An enzyme is a catalytic protein. Hydrolysis of sucrose by

More information

Molecular Cell Biology

Molecular Cell Biology Harvey Lodish Arnold Berk Paul Matsudaira Chris A. Kaiser Monty Krieger Matthew P. Scott Lawrence Zipursky James Darnell Molecular Cell Biology Fifth Edition Chapter 2: Chemical Foundations Copyright 2004

More information

Name: Hour: Elements & Macromolecules in Organisms

Name: Hour: Elements & Macromolecules in Organisms Name: Hour: Elements & Macromolecules in Organisms Most common elements in living things are carbon, hydrogen, nitrogen, and oxygen. These four elements constitute about 95% of your body weight. All compounds

More information

Osmosis, Diffusion and Cell Transport

Osmosis, Diffusion and Cell Transport Osmosis, Diffusion and Cell Transport Types of Transport There are 3 types of transport in cells: 1. Passive Transport: does not use the cell s energy in bringing materials in & out of the cell 2. Active

More information

Cellular Respiration & Metabolism. Metabolism. Coupled Reactions: Bioenergetics. Cellular Respiration: ATP is the cell s rechargable battery

Cellular Respiration & Metabolism. Metabolism. Coupled Reactions: Bioenergetics. Cellular Respiration: ATP is the cell s rechargable battery Cellular Respiration & Metabolism Metabolic Pathways: a summary Metabolism Bioenergetics Flow of energy in living systems obeys: 1 st law of thermodynamics: Energy can be transformed, but it cannot be

More information

Membrane Structure and Function

Membrane Structure and Function Membrane Structure and Function Part A Multiple Choice 1. The fluid mosaic model describes membranes as having A. a set of protein channels separated by phospholipids. B. a bilayer of phospholipids in

More information

CHAPTER 6 AN INTRODUCTION TO METABOLISM. Section B: Enzymes

CHAPTER 6 AN INTRODUCTION TO METABOLISM. Section B: Enzymes CHAPTER 6 AN INTRODUCTION TO METABOLISM Section B: Enzymes 1. Enzymes speed up metabolic reactions by lowering energy barriers 2. Enzymes are substrate specific 3. The active site in an enzyme s catalytic

More information

Previous lecture: Today:

Previous lecture: Today: Previous lecture: The energy requiring step from substrate to transition state is an energy barrier called the free energy of activation G Transition state is the unstable (10-13 seconds) highest energy

More information

Preliminary MFM Quiz

Preliminary MFM Quiz Preliminary MFM Quiz 1. The major carrier of chemical energy in all cells is: A) adenosine monophosphate B) adenosine diphosphate C) adenosine trisphosphate D) guanosine trisphosphate E) carbamoyl phosphate

More information

Enzymes and Metabolic Pathways

Enzymes and Metabolic Pathways Enzymes and Metabolic Pathways Enzyme characteristics Made of protein Catalysts: reactions occur 1,000,000 times faster with enzymes Not part of reaction Not changed or affected by reaction Used over and

More information

7. A selectively permeable membrane only allows certain molecules to pass through.

7. A selectively permeable membrane only allows certain molecules to pass through. CHAPTER 2 GETTING IN & OUT OF CELLS PASSIVE TRANSPORT Cell membranes help organisms maintain homeostasis by controlling what substances may enter or leave cells. Some substances can cross the cell membrane

More information

Cell Transport and Plasma Membrane Structure

Cell Transport and Plasma Membrane Structure Cell Transport and Plasma Membrane Structure POGIL Guided Inquiry Learning Targets Explain the importance of the plasma membrane. Compare and contrast different types of passive transport. Explain how

More information

Chapter 3 Molecules of Cells

Chapter 3 Molecules of Cells Bio 100 Molecules of cells 1 Chapter 3 Molecules of Cells Compounds containing carbon are called organic compounds Molecules such as methane that are only composed of carbon and hydrogen are called hydrocarbons

More information

Energy Production In A Cell (Chapter 25 Metabolism)

Energy Production In A Cell (Chapter 25 Metabolism) Energy Production In A Cell (Chapter 25 Metabolism) Large food molecules contain a lot of potential energy in the form of chemical bonds but it requires a lot of work to liberate the energy. Cells need

More information

The amount of cellular adenine is constant. -It exists as either ATP, ADP, or AMP (the concentration of these vary)

The amount of cellular adenine is constant. -It exists as either ATP, ADP, or AMP (the concentration of these vary) Electron transport chain Final stage of aerobic oxidation! Also known as: -oxidative phosphorylation(when coupled to ATP synthase) -respiration (when coupled to ATP synthase) Purpose: -Recycle reduced

More information

Chapter 36: Resource Acquisition & Transport in Vascular Plants

Chapter 36: Resource Acquisition & Transport in Vascular Plants Chapter 36: Resource Acquisition & Transport in Vascular Plants 1. Overview of Transport in Plants 2. Transport of Water & Minerals 3. Transport of Sugars 1. Overview of Transport in Plants H 2 O CO 2

More information

Anabolic and Catabolic Reactions are Linked by ATP in Living Organisms

Anabolic and Catabolic Reactions are Linked by ATP in Living Organisms Chapter 5: Microbial Metabolism Microbial Metabolism Metabolism refers to all chemical reactions that occur within a living a living organism. These chemical reactions are generally of two types: Catabolic:

More information

CHAPTER 3 THE CHEMISTRY OF ORGANIC MOLECULES

CHAPTER 3 THE CHEMISTRY OF ORGANIC MOLECULES CHAPTER 3 THE CHEMISTRY OF ORGANIC MOLECULES 3.1 Organic Molecules The chemistry of carbon accounts for the diversity of organic molecules found in living things. Carbon has six electrons, four of which

More information

Summary of Metabolism. Mechanism of Enzyme Action

Summary of Metabolism. Mechanism of Enzyme Action Summary of Metabolism Mechanism of Enzyme Action 1. The substrate contacts the active site 2. The enzyme-substrate complex is formed. 3. The substrate molecule is altered (atoms are rearranged, or the

More information

1. A covalent bond between two atoms represents what kind of energy? a. Kinetic energy b. Potential energy c. Mechanical energy d.

1. A covalent bond between two atoms represents what kind of energy? a. Kinetic energy b. Potential energy c. Mechanical energy d. 1. A covalent bond between two atoms represents what kind of energy? a. Kinetic energy b. Potential energy c. Mechanical energy d. Solar energy A. Answer a is incorrect. Kinetic energy is the energy of

More information

1. Enzymes. Biochemical Reactions. Chapter 5: Microbial Metabolism. 1. Enzymes. 2. ATP Production. 3. Autotrophic Processes

1. Enzymes. Biochemical Reactions. Chapter 5: Microbial Metabolism. 1. Enzymes. 2. ATP Production. 3. Autotrophic Processes Chapter 5: Microbial Metabolism 1. Enzymes 2. ATP Production 3. Autotrophic Processes 1. Enzymes Biochemical Reactions All living cells depend on biochemical reactions to maintain homeostasis. All of the

More information

PRESTWICK ACADEMY NATIONAL 5 BIOLOGY CELL BIOLOGY SUMMARY

PRESTWICK ACADEMY NATIONAL 5 BIOLOGY CELL BIOLOGY SUMMARY Name PRESTWICK ACADEMY NATIONAL 5 BIOLOGY CELL BIOLOGY SUMMARY Cell Structure Identify animal, plant, fungal and bacterial cell ultrastructure and know the structures functions. Plant cell Animal cell

More information

Lecture 4 Enzymes Catalytic proteins. Enzymes. Enzymes 10/21/10. What enzymes do therefore is:

Lecture 4 Enzymes Catalytic proteins. Enzymes. Enzymes 10/21/10. What enzymes do therefore is: Lecture 4 Catalytic proteins Are a type of protein that acts as a catalyst-speeding up chemical reactions A catalyst is defined as a chemical agent that changes the rate of a reaction without being consumed

More information

A disaccharide is formed when a dehydration reaction joins two monosaccharides. This covalent bond is called a glycosidic linkage.

A disaccharide is formed when a dehydration reaction joins two monosaccharides. This covalent bond is called a glycosidic linkage. CH 5 Structure & Function of Large Molecules: Macromolecules Molecules of Life All living things are made up of four classes of large biological molecules: carbohydrates, lipids, proteins, and nucleic

More information

Carbon Hydrogen Oxygen Nitrogen

Carbon Hydrogen Oxygen Nitrogen Concept 1 - Thinking Practice 1. If the following molecules were to undergo a dehydration synthesis reaction, what molecules would result? Circle the parts of each amino acid that will interact and draw

More information

Biological molecules:

Biological molecules: Biological molecules: All are organic (based on carbon). Monomers vs. polymers: Monomers refer to the subunits that, when polymerized, make up a larger polymer. Monomers may function on their own in some

More information

Ionization of amino acids

Ionization of amino acids Amino Acids 20 common amino acids there are others found naturally but much less frequently Common structure for amino acid COOH, -NH 2, H and R functional groups all attached to the a carbon Ionization

More information

ATP accounting so far ELECTRON TRANSPORT CHAIN & CHEMIOSMOSIS. The Essence of ETC: The Electron Transport Chain O 2

ATP accounting so far ELECTRON TRANSPORT CHAIN & CHEMIOSMOSIS. The Essence of ETC: The Electron Transport Chain O 2 accounting so far The final stage of cellular respiration: ELECTRON TRANSPORT CHAIN & CHEMIOSMOSIS Glycolysis 2 Kreb s cycle 2 Life takes a lot of energy to run, need to extract more energy than 4! There

More information

Multiple Choice Identify the choice that best completes the statement or answers the question.

Multiple Choice Identify the choice that best completes the statement or answers the question. AP bio fall 2014 final exam prep Multiple Choice Identify the choice that best completes the statement or answers the question. 1. According to the first law of thermodynamics, a. the energy of a system

More information

CHM333 LECTURE 13 14: 2/13 15/13 SPRING 2013 Professor Christine Hrycyna

CHM333 LECTURE 13 14: 2/13 15/13 SPRING 2013 Professor Christine Hrycyna INTRODUCTION TO ENZYMES Enzymes are usually proteins (some RNA) In general, names end with suffix ase Enzymes are catalysts increase the rate of a reaction not consumed by the reaction act repeatedly to

More information

Electron transport chain, oxidative phosphorylation & mitochondrial transport systems. Joško Ivica

Electron transport chain, oxidative phosphorylation & mitochondrial transport systems. Joško Ivica Electron transport chain, oxidative phosphorylation & mitochondrial transport systems Joško Ivica Electron transport chain & oxidative phosphorylation collects e - & -H Oxidation of foodstuffs oxidizes

More information

Cellular Respiration and Fermentation

Cellular Respiration and Fermentation LECTURE PRESENTATIONS For CAMPBELL BIOLOGY, NINTH EDITION Jane B. Reece, Lisa A. Urry, Michael L. Cain, Steven A. Wasserman, Peter V. Minorsky, Robert B. Jackson Chapter 9 Cellular Respiration and Fermentation

More information

ATP Synthesis. Lecture 13. Dr. Neil Docherty

ATP Synthesis. Lecture 13. Dr. Neil Docherty PG1005 The Electron Transport Chain and ATP Synthesis Lecture 13 Dr. Neil Docherty My Teaching Objectives Define and describe the electron transport chain Explain how electron transfer couples to proton

More information

Passive and Active Transport 1. Thermodynamics of transport 2. Passive-mediated transport 3. Active transport

Passive and Active Transport 1. Thermodynamics of transport 2. Passive-mediated transport 3. Active transport Passive and Active Transport 1. Thermodynamics of transport 2. Passive-mediated transport 3. Active transport neuron, membrane potential, ion transport Membranes Provide barrier function Extracellular

More information

1. The diagram below represents a biological process

1. The diagram below represents a biological process 1. The diagram below represents a biological process 5. The chart below indicates the elements contained in four different molecules and the number of atoms of each element in those molecules. Which set

More information

Lecture Overview. Hydrogen Bonds. Special Properties of Water Molecules. Universal Solvent. ph Scale Illustrated. special properties of water

Lecture Overview. Hydrogen Bonds. Special Properties of Water Molecules. Universal Solvent. ph Scale Illustrated. special properties of water Lecture Overview special properties of water > water as a solvent > ph molecules of the cell > properties of carbon > carbohydrates > lipids > proteins > nucleic acids Hydrogen Bonds polarity of water

More information

Enzymes and Metabolism

Enzymes and Metabolism Enzymes and Metabolism Enzymes and Metabolism Metabolism: Exergonic and Endergonic Reactions Chemical Reactions: Activation Every chemical reaction involves bond breaking and bond forming A chemical reaction

More information

Chemistry 20 Chapters 15 Enzymes

Chemistry 20 Chapters 15 Enzymes Chemistry 20 Chapters 15 Enzymes Enzymes: as a catalyst, an enzyme increases the rate of a reaction by changing the way a reaction takes place, but is itself not changed at the end of the reaction. An

More information