3D Cell Culture mimsys G

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Loren Golden
8 years ago
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1 3D Cell Culture mimsys G xeno-free & nutrient permeable hydrogel for 3D cell culture mimsys G is a xeno-free and non-immunogenic, easy to handle hydrogel for cell encapsulation in 3D experiments in vitro or in vivo. mimsys G is nutrient permeable and allows long term 3D cell culture, at least 21 days of proliferation and survival The transparency of mimsys G hydrogel make it suitable for established laboratorial cell assays, such as cell differentiation, microscopy evaluations, viability protocols and histological procedures Parque da Ciência e Tecnologia Avepark Zona Industrial da Gandra GMR Portugal Phone Fax

2 mimsys G Ref Characteristics Xeno-free Transparent Water soluble Defined composition: methacrylated gellan gum is based on gellan gum wich is extensively used on food industry providing no-cytotoxicity and noimmunogenic response Gelation in less than 5 min by ionic crosslink with cationic solution (such as media or PBS) Gelation by photo-initiation with UV light for higher spacial and temporal control Easy to handle at room temperature and at 37ºC Allows oxygen and nutrient flow through the hydrogel up to the cells Intended use: for research use only Benefits mimsys G accurately mimics the 3D cell environment: Cell-to-cell communication are improved Cell morphology is closer to its native one Right flow of nutrients through the hydrogel No necrosis: oxygen can naturally penetrate the gel High reproducibility of prepared hydrogels due to the robustness and easy handling of the product Compatible with stem cells and cell lines 3D culture for long term culture Easy to use in in vivo experiments Appropriate for established downstream applications: Optical microscopy observation of your 3D cell culture Viability assay, such as MTS Fluorescence microscopy for Live/Dead and morphology assays Histology assays of hydrogels from in vivo studies Other application range: drug delivery, diagnostic marker delivery and surface coatings Save time in your research: Fast gelation time 5 min Save money in your experiment: 1 vial suitable for 2x96 well plates Cell morphology in long term culture (21 days) High cell viability Costumize 3D hydrogels with mimsys G Customize your experiment! Hydrogel with the shape and volume that you want (well-plates, petri-dish, etc) Easy supplementation with growth factors, matrix proteins, or other molecules specific for your work Injectable hydrogel with in situ gelation to improve the control in your in vivo studies Related products hsvf human Stromal Vascular Fraction hasc Stromal Cells hasc xeno-free Stromal Cells xeno-free hsvf xeno-free human Stromal Vascular Fraction xeno-free

3 Xeno-free human Stromal Vascular Fraction hsvf xeno-free Heterogeneous cell population isolated from adipose tissue processing Adipose tissue processing, cell isolation and cryopreservation are performed under Good Manufacturing Practices equivalent conditions (cgmp), using xeno-free and GMP reagents Parque da Ciência e Tecnologia Avepark Zona Industrial da Gandra GMR Portugal Phone Fax

4 hsvf xeno-free Ref Brief Description Cells of each hsvf lot are from a single adult donor of human lipoaspirate tissue hsvf contains adherent and nonadherent cells including endothelial (progenitor) cells, smooth muscle cells, fibroblasts, lymphocytes, pericytes, monocytes, macrophages, preadipocytes, progenitor cells, as well as mesenchymal / hematopoietic stem cells Vials are provided with indication of donor age, gender, ethnicity, Body Mass Index (BMI) and anatomical harvesting location Intended use: for research use only Benefits Minimally manipulated cell source that enables researchers more experimental freedom and substantially reduces dependence on isolation criteria High quality control analysis Extensive information regarding cell characteristics is provided hsvf cultured in 2D cell culture plasticware Characteristics 1x10 6 viable cells/ml (post-thawing) Minimally manipulated: non-passaged cells hsvf cultured in 3D mimsys G hydrogel Xeno-free: processing and cryopreservation medium Each hsvf lot is characterized by flow cytometry analysis for: Viability Total cell number Presence / absence of cell-surface markers: CD31, CD34, CD45, CD73, CD90 and CD105 Also available non xeno-free Related products mimsys G 3D cell culture hydrogel hsvf human Stromal Vascular Fraction hasc Stromal Cells hasc xeno-free Stromal Cells xeno-free

5 Xeno-free human Adipose derived Stem/ stromal Cells hasc xeno-free Obtained from expansion of human Stromal Vascular Fraction (hsvf), isolated from adipose tissue processing Adipose tissue processing, cell isolation and cryopreservation are performed under Good Manufacturing Practices (cgmp), using xeno-free and GMP reagents Parque da Ciência e Tecnologia Avepark Zona Industrial da Gandra GMR Portugal Phone Fax

6 hasc xeno-free Ref A Brief Description Cells of each hasc lot are from a single adult donor of human lipoaspirate tissue hasc are less heterogeneous than hsvf cells, yet not a homogeneous cell population hasc include multipotent cells with the ability to differentiate into osteoblasts, adipocytes and chondrocytes, among other lineage pathways. Vials are provided with indication of donor age, gender, ethnicity, Body Mass Index (BMI) and anatomical harvesting location Characteristics 1x10 6 viable cells/ml (post-thawing) hasc are obtained and characterized in BSL-2 laboratory Each hasc lot is characterized by flow cytometry analysis for: Viability Total cell number Presence / absence of cell-surface markers: CD31, CD34, CD45, CD73, CD90 and CD105 Intended use: for research use only Benefits Cell manufacturing in xeno-free conditions High quality control analysis Extensive information regarding cell characteristics is provided B C Xeno-free: processing and cryopreservation medium Related products Stage of culture: cryopreserved cells in passage 1 (2 nd culture) Tri-lineage Differentiation: Adipogenesis, Chondrogenesis and Osteogenesis Also available non xeno-free Tri-lineage differentiation potential of hasc A Osteogenesis: Alizarin red staining of mineralized extracellular matrix (ECM) B Adipogenesis: Oli red O staining of lipid vacuoles C Chondrogenesis: Alcian blue staining of ECM proteoglycan (pellet cross-section) mimsys G 3D cell culture hydrogel hsvf human Stromal Vascular Fraction hasc Stromal Cells hsvf xeno-free human Stromal Vascular Fraction xeno-free

protocol handbook 3D cell culture mimsys G hydrogel

protocol handbook 3D cell culture mimsys G hydrogel handbook 3D cell culture mimsys G hydrogel supporting real discovery handbook Index 01 Cell encapsulation in hydrogels 02 Cell viability by MTS assay 03 Live/Dead assay to assess cell viability 04 Fluorescent