HISTO SPOT SSO System
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1 HISTO SPOT SSO System for HLA Typing HISTO SPOT Kits MR.SPOT Processor HISTO MATCH Software Complete system certified for IvD use
2 HISTO SPOT Features The HISTO SPOT SSO assay in combination with the MR.SPOT processor represents the most automated typing system for HLA typing on the market. Test principle: Miniature reverse SSO blot DNA Extraction Advantages: Fully automated SSO assay Flexible: 1 to 96 tests per run Fast: 3 hours from sample to result for a single type Locus-specific amplification with biotinylated primers in a standard PCR Easy to handle Plug & play instrument Ready to use reagents No extra washing runs necessary Convenient interpretation software included Hybridisation of the amplicon to SSO probes fixed on the bottom of the well of a microwell plate: There is one test per well! Superior resolution with the new HISTO SPOT 4D kits With HISTO SPOT, on-call testing can now be automated because of the speed of the process in combination with a very low hands-on time and simple test procedure. The flexibility and convenience of the system makes HISTO SPOT the ideal solution for low as well as high throughput requirements. Colourmetric reaction to detect positive probes The resulting pattern of positive probes is interpreted as HLA-Genotype
3 MR.SPOT Processor The MR.SPOT processor runs the whole assay from amplicon to result. After PCR amplification, the amplicon tray is placed into MR.SPOT. It adds hybridization buffer to each well and transfers each amplicon together with the hybridization buffer to the test well containing the immobilized SSO probes. Then the processor performs all necessary washing steps and all pipetting steps for the colorimetric reaction. Afterwards the resulting probe pattern (coloured dots in the bottom of each well) is photographed by the integrated camera and the image is transferred to the interpretation software. The MR.SPOT Software guides the user through all steps: Just follow the instructions on the screen and press the start button
4 HISTO SPOT HISTO MATCH: Create and send worklist MR.SPOT : Create/ import samples Create worklist Send worklist Setup PCR
5 System Workflow SSO assay HISTO MATCH: Return and type Import worklist Automated interpretation Review results Report results
6 HISTO MATCH Software This user friendly software is the central workstation and is designed for Patient and sample management Organization of the workflow and sample tracking Creating worklists Interpretation of the results Typing reports Data Management Microsoft SQL Server 2005 Database manages patients, samples, worklists, users, processors and kits. Processors Kits Worklists Samples Patients Colour coded background makes navigation through the software very easy!
7 Clearly arranged interpretation screen Interpretation options and features: Gridded result: Array view Common allele filter NMDP codes Activate / inactivate probes Automatically ignore 0-25 probes Change cut-off values Add / remove results View array picture Add comments Complete editing history Authorize results Rare alleles are indicated Amplification failures are indicated Probe performance statistics are accessible from the interpretation screen Report options : Compact report Low resolution report High resolution report Patients and sample report Export to text file (.csv) SCORE export
8 HISTO SPOT SSO Kits Locus Specific Typing Kit Reagent Kit Mastermix (colour coded lid according to HLA locus) MgCl 2 Typing wells (colour coded on the bottom according to HLA locus) Colour coded reagents for the SSO assay Barcoded lot numbers for easy traceability Ready to use reagents Storage of all reagents at 4-8 C Storage of all reagents at 4-8 C HISTO SPOT 4D Kits The HISTO SPOT typing kits will be transformed to 4D kits as soon as they reach four digit resolution for a defined set of common alleles. For further information visit our website at BAG Health Care GmbH Amtsgerichtsstraße Lich/Germany Tel.: +49 (0) 6404/925-0 Fax: +49 (0) 6404/ info@bag-healthcare.com Auftragsannahme/Ordering: Tel.: +49 (0) 6404/ Fax: +49 (0) 6404/ verkauf@bag-healthcare.com Customer Service: Tel.: +49 (0) 6404/ Fax: +49 (0) 6404/ service@bag-healthcare.com
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