EUROPEAN COMMISSION HEALTH & CONSUMER PROTECTION DIRECTORATE-GENERAL. Unit 04 - Veterinary Control Programmes

Transcription

1 *** * * *** EURPEAN CMMSSN HEALTH & CNSUMER PRTECTN DRECTRATE-GENERAL Unit 04 - Veterinary Control Programmes SANC/0285/2007 Programmes for the eradication and monitoring of animal diseases, of certain TSEs and for the prevention of zoonoses Eradication programme of Rahies Approved* for 2007 by Commission Decision 2006/875/EC Czech Republicl * in accordance with Commission Decision 90/424/EEC Commission européenne, B-1049 Bruxelles Europese Commissie, B-1049 Brussel- Belgium. Telephone: {32-2)

2 .,... '.J' STATE VETERNARY ADMNSTRATN Czech Republic Slezská 7, PRAHA 2 Tel.: Fax.: Your letter did Your reference ur reference : VET Attachement File handled by Department Phone Fax Dr. Satrán European Commission Health and Consumer Protection Directorate-General E2 - Animal health and welfare, zootechnics Mr. Bernard Van Goethem - Head of Unit. F3/56 B-049 Brussels, BELGUM Re: Programme for the control of Rabies in the year 2007 Prague Dear Sir, Enclosed please find the programme for the control of Rabies in the Czech Republic in the year 2007 as requested in the Commission Decision 450/2004/EC. The electronic version of the programme was submitted to address [email protected]. Kind regards. Yours sincerely, MVDr. Milan Malena Director General (CV). Enclosure: ala

3 Programme for the control of Rabies in the Czech Republic 1.!Qgramme for the control of Rabies Member State: Czech Republic Disease: Rabies Year of implementation: 2007 Reference ofthis document: State Veterinary Administration of the Czech Republic Contact Name: Phone: Fax: MVDr. Petr Satrán, Ph.D Date sent to the Commission:

4 Programme for the control ofrabies in the Czech Republic 2. Historical data on the epidemiological evolution of the disease: Rabies history The Czech Republic belongs to those countries in which the incidence of rabies in wilc;llife was steadily increasing since the 1950s until 1980s. Continual research carried out during proved that rabies had become enpemic in the border areas of West and North Bohemia and North Moravia. The importance offoxes in rabies epidemiology increased and red fox became the principal vector of rabies in the Czech Republic. Neither subsidiaries payment for hunted foxes, which wasintroduced in 1969, nor gassing of fox dens, carried out during , did not improved the situation. n the 1980s rabies reached its greatest geographical range. With the exception 0f several districts, the whole territory of the Czech Republic was affected. The oral vaccination of foxes was launched in a few districts adjacent to German borders in 1989 and implemented further thereafter. Since that time continual decline has been visible especially since 92 w4en positive effec of oral vaccination has become evident Status of Rabies The rabies data reported during the last thirteen years indicate the development of the rabies situation in our country since the beginning of oral vaccination. n the period 1989 to 2005, animals were examined for rabies. The major part of them were foxes (more. than 50%) followed by cats and dogs participating by 30 % together. Rabies was diagnosed in cases during this thirteen year period. The highest!).umber of rabies cases was recorded in 1989, reaching 1 S cases. The lowest occurrence (3 cases - April) was recorded in n 2004 the Czech Republic obtained a status of a country officially free of rabies. n 2005 one positive bat for rabies was recorded. Rabies in animals belonging to 39 species were examined for rabies in the Czech Republic in nly three rabies cases were diagnosed in foxes from district Trutnov in East ohemia. Rabies in animals belonging to 45 species were examined for rabies in the Czech Republic in No positive case was recorded. Rabies in 2004 n total, animals were examined for rabies in the Czech Republic (22 farm animals, 8549 wildlife animals, 727 pet animals). No positive case was recorded. Rabies in 2005 n total, animals were examined for rabies in the Czech Republic (774 pet animals, 7. farm animals, wild animals). ne positive bat for Lyssavirus (rabies) was recorded. 2

5 Programme for the control of Rabies in the Czech Republic 3. Description of the programme for Rabies control: Domestic animals Preventive vaccination of domestic carnivores and if necessary, domestic herbivores is the principal method of domestic animals protection. The inactivated tissue-culture vaccines are used exclusively for this purpose. Wild animals The strategy of rabies control is based on reduction of wildlife reservoir of the virus by oral vaccination of foxes. ral vaccination of foxes The field trial of oral immunization of foxes was started in the Czech Republic in spring The project planning and organization was prepared according to the rules of the WH, in close co-operation with the Rabies Centre in Tübingen. The first application of the oral rabies vaccine was carried out in the districts Klatovy, Domazlice, Tachov adjacent to German border in the spring n the course of next campaigns the treated area was extended covering 44 districts in autumn n the autumn 1993 the whole territory of the Czech Republic, with exception of rabies free districts bordering Germany, was included. The "Bavarian model" was applied during all vaccination campaigns. The hunters placed the vaccine baits manually in their hunting preserves. The strategy of vaccine baits distribution twice a year in spring and autumn was applied. Veterinary authorities were responsible for coordination and supervision of the campaigns at the district level. The original baits density 16 doses per 1 km2 were gradually increased, mainly in the areas with non-favourable epidemiological situation. Since 1996, the aerial distribution of the vaccine baits was used on restricted territory (4-6 districts). n 2002 the aerial vaccination was extended reaching 50% oftreated territory (29 districts). The aerial vaccination was extended reaching 99% oftreated territory in Nearly twenty five million of vaccine baits was used from 1989 till the spring ' Results of oral vaccination Contol examinations following baits distribution were oriented to baits uptake, rabies diagnosis, tetracycline marking, characterization of virus strains and antibody formation. The indirect measuring of baits uptake was obtained by the examination of fox bones for tetracycline incorporation. ral vaccination had undisputable positive influence on rabies incidence. A considerable reduction of positive findings was noticed, - from 1501 cases started in 1989 to 3 cases in An increase was registered in the year 1999 (214 cases). Since 2003 to 2005 only one positive bat for rabies was recorded. ' 3

6 Programme for the control of Rabies in the Czech Republic 4. Measures of the submitted programme for oral vaccination of foxes 4.1. Summary of measures under the programme Duration of the programme The oral vaccination has been carried out since 1989 and is planning to continue with oral vaccination in future. t is foreseen to create and keep the buffer zone around the borders, to Poland and Slovakia till eradication programme will be finished there. The pre-requisite of stable rabies situation on our territory must be also fulfilled Temporal pattern The spring and autumn campaigns are organized annually. Timing for aerial and manual vaccination: Spring - April Autumn - ctober Vaccines Since 1992, only Czech made live attenuated vaccine SAD - Bern has been used for vaccination campaigns. Producer is Bioveta a.s., vanovice na Hané. More information about the vaccine you can see in the package insert attached in ANNEX V Distribution Aerial distribution - by a fixed-wing plane. Density = baits/krn2. Aerial distribution is the main method for distribution. Each campaign of aerial vaccination is coordinated and supervised by state veterinary inspectors. Each inspector is responsible for area about km2 closed to one airport. The GPS system is used for navigation and flying lines are recorded by computer and printed out on a map. The distance of flight lines is 500 m; the speed is km per hour. The distribution by plane is more efficient than by hand with respect to a long - term vaccination. Better treatment is reached especially in the hilly areas. Manual placement - by voluntary hunters. Density = 18 baits/km2. n the areas with ineffective aerial distribution, the vaccine is placed manually. The exact date and time of the vaccine bait distribution is fixed. Distribution of vaccine to hunters and the control of vaccination is organised at the district level by Veterinary bodies. Manual placement is only a supplementary means used on towns' outskirts, in parks etc Designation of the central authority charged with supervising and coordinating the departments responsible for implementing the programme: The central authority competent for supervising and coordinatingall activities in veterinary care is the State Veterinary Administration, which performs its powers at the whole territory of the Czech Republic Cs 47, Veterinary Act No 166/1999 Col. of Acts). SVA of the CR coordinates the activities of Regional Veterinary Administrations., 4

7 Programme for the control of Rabies in the Czech Republic The Department of Animal Health and Welfare in co-operation with the National reference Laboratory for Rabies will prepare a concrete plan of the oral vaccination according the present rabies situation. This plan is approved by the State Veterinary Administration afterwards. Regular meetings are organised with the participation of the representatives of the air-company, the vaccine manufacturer and the representatives of the Regional eterinary Administrations from districts which are involved in the vaccinated area. The exact timetable and further details are discussed at these meetings, such as:. Distribution of the vaccne and its storage before vaccination and the. delivery conditions to the airport (storage at -200 C).. n the districts, where the vaccine is distributed manually the exact date and time of the vaccine bait çlistribution is fixed. The distribution to hunters and control of the vaccination is organised at the district level by Veterinary bodies. A vaccine producer is selected in the tendering procedure. This company also ensured the aerial distribution of vaccine baits in current year. Manual distribution is carried out by hunting organisations in compliance with the instructions of the Regional Veterinary Administration Description and delimitation of the geographical and administrtive areas in which the programme is to be applied: The planned extent ofbuffer zone (spring and autumn): Area to be covered*. Number of doses * ne vaccination campaign only For information about regional distribution see annex km baits 4.4. System in place for the registration of holdings: The'program will be performing in wild life population of foxes System in place for identification of animals: The program will perform in wild life population offoxes without any identification bftarget animals Measures in place as regards the notification of the disease: n the case of Rabies positive results the protective and control measures will be imposed. according to the provisions of the Veterinary Act No. 166/1999 às amended. 5

8 Programme for the control of Rabies in the Czech Republic 4.7. Measures and terms of legislation as regards the control vaccination of the disease: Contra of baits acceptance after manual placèment: n. the vaccinating areas, a distribution team checks the baiting sites on days 4, 8 and 14 following the placement. The number of baits taken is recorded into a protocol. The hunters also look out for animal sings and tracks, teeth marks in vaccine capsules in order to identify the animal species, which took the bait. The results are collected by veterinary inspectors and then are submitted to the National Reference Laboratory for rabies. Follow up examinations: At least 8 -la faxes per 100 km2 per year must be presented for rabies examination and further control investigation. That means about 50 foxes per district after each campaign. Not vaccinated districts have to be also included into the surveillance area. Beside of these, clinically suspect and found dead animals from all over the country should be analysed for rabies. The evaluation of the efficacy includes: - % of the bait uptake.. % of the TTC marker positivity - Asses of the Rabies epidemiological situation 4.8. Measures and terms of legislation as regards the measures in the case of positive results: n the case of Rabies positive results the protective and control measures will be imposed according to the provisions of the Veterinary law 166/1999 as amended Measures and terms of legislation as regards the compensation for owners of slaughtered and killed animals: n the case of Rabies positive results the protective and control measures will be imposed according to the provisions of the Veterinary law 166/1999 (67) as amended and 100% ompensation for owners is established. 6

9 Programme for the control of Rabies in the Czech Republic 5. General description of the costs and benefits. Fi l. C k Year Spring 2007* Autumn 2007 * Tota12007* TDTAL* Price o/baits* Prices are without tax (VAT) 1 = 28, 50 Czk For more details, see ANNEX. Price/or tl'llnspor Price/or laying PriJe/or laying t byairplane* by haml,and storage, Czk ( ,26 ) 6. Data on the epidemiological evolution during the last five years Number or foxes examined per 100 km2 Year Number 0/ examined Number/l km ,6 8,8 11,0 10,4. 11,9 12, Monitoring of wildlife ; 3,00 Animal species: Fox Virological tests, which are used: Direct mmunofluorescent Test and Mouse isolatiqntest. Control of vaccination efficacy is performing by biomarker detection test and Seruin neutralization test on cell culture. 7

10 Programme for the control of Rabies in the Czech Republic Surveillance of Vaccination efficiency in red foxes in 2005 Region Number Positive TTC % f tested Central Bohemia Hradec Králové Karlovy Vary 291 Liberec Moravia-Silesian lomouc Pardubice Pizen 544 Praha 37 South Bohemian 788 South Moravia Ustf nad Labem Vysocina Zlin Total ,5 1, Number of animals, which were tested last 5 years Number of positive sample * * ne positive bat for Lyssavirus (rabies) 8

11 Programme for the contra of Rabies in the Czech Republic 6.3. Data on vaccination of wildlife Number of baits lay down on the whole Czech Republic territory Campaigll Number of baits Area;'1 km Area ill km by Are( ill km- by hllllll airplane Spring :386 Autumn Spring Autumn Spring Autumn Spring Autumn Total F', iipaign---price ofbaits Price per Pricefor layillg Pricefor (l'illg transport allll by a;,plane by hallll storage Spring 2002* Autumn 2002* Spring 2003* Autumn 2003* Spring 2004* Autumn 2004* Spring 2005** Autumn 2005** * Prices including tax (VAT) ** Prices without tax (19% VAT).. '7. Targets Dvaccination ofwildlife o Disease: Rabies Animal species: Fox Number of baits to be laid down on the whole Czech Republic territory Campaign Spring 2007 Autumn 2007 Total2007 Number of baits Area ill knl Area ;', kill Area ill knl by halid by airpltll1e For more details and regional distribution, see table in ANNEX and map in annex 1. ' 9

12 Programme for the control of Rabies in the Czech Republic Testing scheme After each campaign, specimens of wildlife animals are collected within vaccination area for examination, as recommended by WH. At least 8-10 foxes per 100 km2 per annum must be presented for rabies examination and further control investigation. That means about 50 foxes per district after each campaign. No vaccinated districts have to be also included into surveillance area. Beside of these clinically suspect and found dead animals from all over country should be analysed for rabies. Control examination following bait distribution should be directed at bait uptake, rabies diagnosis, tetracycline marking, characterization of virus strains and antibody formation. The indirect measuring of bait uptake is obtained by the examination of fox bones for tetracycline incorporation. Antibody formation is tested in the body fluids. Specific virus neutralizing antibodies are detected Number and specification of tests to be carried out in the year 2007 Region Type of tests Target Type of sample bjective Number of population planned tests Central FAT,SNT,TTC Fox Brain,Blood,Bone monitoring of 950 Bohemia campaigns Hradec Králové FAT,SNT,TTC Fox Brain,Blood,Bone monitoring of 750 campaigns Karlovy Vary FAT,SNT,TTC Fox Brain,Blood,Bone monitoring of 300 campaigns Liberec FAT,SNT,TTC Fox Brain,Blood,Bone monitoring of 550 campaigns Moravia- FAT,SNT,TTC Fox Brain,Blood,Bone monitoring of 890 Silesian campaigns lomouc FAT,SNT,TTC Fox Brain,Blood,Bone monitoring of 320 campaigns Pardubice FAT,SNT,TTC Fox Brain,Blood,Bone monitoring of. campaigns 620 Pzen FAT,SNT,TTC Fox Brain,B 100d,Bone monitoring of 550 campaigns Praha FAT,SNT,TTC Fox Brain,Blood,Bone monitoring of 50 campaigns South FAT,SNT,TTC Fox Brain,Blood,Bone monitoring of 800 Bohe!T\ian campaigns SouthMoravia FAT,SNT,TTC Fox Brain,Blood,Bone monitoring of 620 campaigns Usti nad Labem FAT,SNT,TTC Fox Brain,Blood,Bone monitoring of 650 campaigns Vysotina FAT,SNT,TTC Fox Brain,Blood,Bone monitoring of 500 campaigns Zlin FAT,SNT,TTC Fox Brain,Blood,Bone monitoring of 600 campaigns Total 8150 FAT - Flurescent antibody test SNT -Serum neutralization test on cell culture TTC - Tetracycline marker test 10

13 Programme for the control of Rabies in the Czech Republic 8. Detailed analysis of the cost of the programme 8.1. Summary of the costs Costs relatedto... Specification Number of 1.1. Purchase of vaccine Spring campaign 2. Distribution costs 3. Administering costs Autumn campaign Spring by airplane Spring by hand Autumn by airplane Autumn by hand Price for transport and storage - Spring Price for transport and storage - Autumn.. Unitary cost ill Total (l1ount in Czk Community funding units Czk requested 1. Vaccination baits baits km2 133 km km2 133 km Yes Yes Yes Yes Yes Yes No No No 1 =28,50 Czk 4. Control costs. Prices are withouttax Total Czk ( , 26 ) No Yes 11

14 12/06/2006 rálni vakcinace lisek rok l, kampan jaro "PliE letecky ruë celkern BN Benesov i BE Beroun BK Blansko i BM Bmo-mesto B Bmo-venkov BR Bruntál i BV Bì'eclav Ceská Lipa J CB Ceské Budejovice CKTCesky Krumlov : DC Decin D Domazlice FM FrYdek-Mistek HB Havl[ckuv Brod H Hodonin i HK Hradec Králové CH Cheb CV Chomutov! CR Chrudim JN Jablonec nad Nisou JE Jesenik 720 i 720 JC Jicfn' 887 -l- ' 887 J Jihlava i JH Jindì'ichuv Hradec i KV KarlovyVary l K Karviná 347 i 347 KL Kladno i KT Klatovy K Kolin. L. KM Kromeì'iz 900! 900 KH Kutná Hora i -' lo! r j l ' i : ! i.10 l t Q._ J! i ANNEX 2007; vkládaci cást strana 1 z 8

15 12/06/2006 rálni vakcinace lisek rok 2006 kampan jaro LB Liberec 1Tl Litomêrice LN Louny ME Mêlnik MB Mladá Boleslav M Most NA Náchod NT Novy Jicin NB Nymburk L lomouc P pava S strava-mêsto PU Pardubice PE Pelhrimov P Pisek Pl Plzen-jih PM Plzen-mêsto PS Plzen-sever AB Praha-mêsto PY Praha-vychod PZ Praha-západ PT' Prachatice PV Prostêjov PR Prerov PB Pribram letecky rucné L celkern 800, l ol ol ol ol ol olof ol l l o, l ol =j ol. ol o' ol l ol ol ol ol 0--1 ol '. J ol ol ol ol. l', -o ol ol ol ANNEX 2007; vkládaci Cást strana 2 z 8

16 12/06/2006 rálni vakcinace lisek ' rok 2006 kampari jaro letecky rucne celkern r1 Rakovnik R Rokycany RK Rychnov nad Knêinou SM Semily S Sokolov ST Strakonice Sy Svitavy SU Sumperk 1.315' TA Tábor TC Tachov TP Teplice TU Trutnov TR Hebic. l. UH bherské Hradistê l l. UL Usu nad Labem U UsUnad rlici rl-- VS VseUn VY Vyskov ZL Zlin ZN Znojmo ZR Zcfár nad Sázavou.... èeská republika hranice SRN hranice PL hranice SK hranice AUT ANNEX 2007; vkládaci cást strana 3 z 8

17 12/06/2006 rálni vakcinace isek rok 2006 kampan jaro letecky ruènè celkern poèet dávek na km2 pri leteckém kladeni poèet dávek na km2 pri ruènirn kladeni 18 ANNEX 2007; vkládaci èást strana 4 z 8

18 12/06/2006 cena za dávku 12,90 Kei cena za 1 km2 letecky 126,00 K9 cenaza 1 km2rucnè L 50,00Kei l _ ANNEX 2007; vkládaci eást strana 5 z 8

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22 12/06/2006. rálni vakcinace lisek rok 2006 kampan jaro."..r. BN L!!enesov BE Beroun BK Blansko sto B Brno-venkov BR Bruntál Breclav Cl Ceská Upa CB Ceské Budejovice Ck- -Cesky Krumlov DC D FM HB Deein Domazlice FrYdek-Mistek Havlieküv Brod H Hodonin HK Hradec Králové CH Che CV Chomutov CR Chrudim Jablonec nad Nisou Jesenik JC, Jiein J Jihlava JH Jindrichuv Hradec KV Karlovy Vary K Karviná Kl Kladno KT Klatovy KC Kolin KM Kromeriz KH Kutná Hora letecky ol ol ol ol ol ol / Ò ol ol ol ol j ol ol ol ol l ol ol ol ol LT.o- -::..:::() ol l ol l ANNEX 2007; vztek strana 1 z 8

23 =::vbesto /06/2006 rálni vakcinace lisek rok, 2006 kampan jaro i L --=-- == ) 3r-1 13 PE Pelhfimov --i ! P 'Pisek 'PJ! Plzeri-jih --j imtpïzer\=mesto :;o -Ô letecky ruènê celkem _+ LB ' Liberec ; LT ' Litomefice : ' o '_ ' --' LN Louny o ME Melnik =t-.- ; Q: MB Mladá Boleslav 1:Q i M Most i ' ---j NA, Náchod NJ Novy Jicin -; gß7 - --:-- -=-=--=-=t= ' P : pava l 1.144!:- - -]_ --:.- _:-- -] Y_ Praha=vchodn.J. Q._.. _ Q n PZ Praha-záJ!... :_ PT Prachatice. t _ u PV l Prostejov ,. --T _'n _fj!ib..rélm PR Pferov ' 'ô , L_ "; t = _=_: _., u _. - Q ; ' ' ö _T _= ; L.- 0_ r r f---- : ' ; ' -- r , ANNEX 2007; vztek strana 2 z 8

24 12/06/2006 rálni vakcinace lisek rok 2006 kampan jaro RA Rakovnik Rokycany RK Rychnov nad Knêinou SM Semily 50 Sokolov ST Strakonice 5Y Svitavy SU Sumperk ill Tábor TC Tachov TP Teplice TU Trutnov Tì'ebic UH Uherské Hradistê UL UsU nad Labem U UsU nad rlici V5 VseUn VY Vyskov ZL Zlin ZN Znojmo ZR Zd Sázavou ëeská republika. hranice 5RN --- hranice PL hranice 5K hraniceaut letecky rucnë L l D _ celkern l ol i ol l l , ANNEX 2007; vztek strana 3 z 8

25 12/06/2006 rálni vakcinace lisek rok 2006 kampalï jaro leteck_uënê l-- c::!_ke oëet dávek na k!!,ri em klade,!i '_ J 3 e d".e.!!a m2 i_!,im k!adeni =1 "-8 ANNEX 2007; vztek strana 4 z 8

26 12/06/2006 cena za dávku 12,90 Kël cena za 1 km21etecky 126,00 Kël cena za 1 km2 ruènè 50,00 Kël ANNEX 2007; vztek strana 5 z 8

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30 " Rabies ral Vaccination of Foxes from to from 1 to from to from to _ from to E.:: ;::.:l overthan _ ANNEX3 ANNEX 200?mapa

31 12/06/2006 rálni vakcinace lisèk rok 2007 kampari Spring kres letecky ol ol ol ol 3tljBeneSov BE Beroun l i BK Blansko BM Brno-mesto B Brno-venkov BR Bruntál BV Bfeclav J Cl Ceská Upa CB Ceské Budejovice 1= _ CK Cesky Krumlov DC Deein _ D Domazlice FM FrYdek-Mistek HB Havliekuv Brod H Hodonin HK Hradec Králové CH Cheb' CV Chomutov CR Chrudim l J JN Jablonec nad Nisou _ JE Jesenik J.C Jiein = J Jihlava! i JH Jindnchùv Hradec KV Karlovy Vary K Karviná Kl Kladno 0 _ KT Klatovy - K Kolin :-- f n - - KM Kromeriz _ Ö KH Kutná Hora laf loa SPRNG ANNEX l and ;vkládaci eást strana 1 z 8

32 l -- 12/06/2006 rálni vakcinace lisek r rok' 2007 : kampan Spring -:-t --- u i-!!c!<y_ L ru'!ll 800. u_ n t :fice --1 "-- j-'- ME Melnik! u uu _, t , o-o.. _ ' o il r::oleslav :: t 1.:: f-- }= '- r-u--- _J-- l ::::: NJ Novy Jicin i =rr=- ---_-g== i _t=: - P pava u ' : : :to P Pisek - -- n_ - - ö- -,--- i =-13 u _n n_t--- r 13 f=1=_4 F-.-T! -'0-0--'--- -o : PJ Plzen-jih _ L Q. : u Q. 9 : o 2.40 PM Plzen-mesto Q Q.!.Qu Q._ u <l PS Plzen-sever..Q..9. Q '. _ :::d i- PZ'+Praha-západ - n - - -i r---t --- no _Q K-l _9. j! _-;- o-l --ö Q _ i PT. Prachatice.' - : - "t l--- _ PV Prostejov 120_..: _ : L 120 l _ }.ooq 9 3.0Q + PR Pferov _: PB : Pfibram =J _ 884 Q _ ; 884 i!j _:..un 9 _ n' n 9 L_ 0.-l. 9 ' i SPRNG ANNEX l and 2007; vkládaci cást strana 2 z 8

33 12/06/2006 rálni vakcinace lisek rok 2007 kampari Spring letecky ruènë celkem RA Rakovnik T T T R Rokycany RK Rychnov nad Knèfnou SM Semily S Sokolov ST Strakonice SV Svitavy SU -Sumperk TA Tábor TC Tachov TP Teplice TU Trutnov TR Trebie UH Uherské Hradistè stinad Labem U Usti nad rlici VS Vsetin VY Vyskov ZL Zlin ZN Znojmo ZR Zel'ár nad Sázavou.1 Ceská republika ] hranice SRN hranice PL hranice SK hranice AUT SPRNG ANNEX l and 2007; vkládaci eást strana 3 z 8

34 . poceáve 12/06/2006 rálni vakcinace lisek rok 2007 kampari Spring "P's letecky rucnë + ekem _ 2S : Q _ 1pocet dávek na km2 l'ri ltedeni na km2 pri ru nimél_c!ni ---j _ 18. SPRNG ANNEX l and 2007; vkládaci cást strana 4 z 8

35 12/06/2006 cena za dávku 13,00Kël cena za 1 km21etecky L 115,00Kël 'cena za 1 km2 ruent} 50,00 Kël 'kurs za 1 Euro 28,50 Kë/1 EUR SPRNG ANNEX l and 2007; vkládaci ëást strana 5 z 8

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39 lorálni vakcinace isek rok 2007 kampan Spring okres letecky ruènê celkern BN Benesov BE Beroun KL Kladno K Kolin KH Kutná Hora ME Melnik MB Mladá Boleslav NB Nymburk py Praha-vychod PZ Praha-západ PB Pfibram RA Rakovnik SPRNG ANNEX l and ;automaticky vypocet strana 1 z 6

40 rok 2007 kampari Spring kr_ -- - letec CB èeské Budejovice Ci<jèeSkY Krumlov u. - --=_Qn JH Jindì'ichuvHradec t ruënê -- celkern. - o- P Pisek, PT Prachatice ST! Strakoniêe - - -j-- TA,Tábor 1 o 0--:- ' - m u : T : m---r--..1e-=-t1 t---' ---nl T-- : , ' ' D KT PJ PM PS R TC okres Domazlice Klatovy Plzen-jih Plzen-mesto Plzen-sever Rokycany 'Tachov letecky i l i.-=- --=-._-= 1== or o o o-j o --o _. r- -=-,.%f -- i=---m! ,n i o o '. o _ o,-=o i o o j J!. 1 (j' ruënê celkern _.1 _.. u'._ ' Ò -t _ l : SPRNG ANNEX l and 2007; automaticky vypocet strana 2 z 6

41 rok 2007 kampan Spring. okres letecky ruènê celkern DC Dêèin CV Chomutov lt Litomêì'ice ln Louny M Most TP Teplice Ul UsU nad Labem okres letecky ruènê celkern Cl èeská Lipa JN Jablonec nad Nisou lb Liberec SM Semily okres letecky ruènê celkern HK Hradec Králové JC Jièin NA Náchod RK Rychnov nad Knêinou TU Trutnov SPRNG ANNEX l and 2007; automaticky vypoèet strana 3 z 6

42 rok 2007 J kampan Spring _ okres CR Chrudim -{ice et_ecky l - -o n - --, U Usti nad rlici i 1.26,! : r r j-- ' Q _ i! ; "-- r Jihlava _Q._; _ L.....Q--t- Q_..l Q._:.9. : okres : : èsto _ letecky rutnê 1 celkern ill' T---% r u _u _" n"_ - - B Brno-venkov _LB!c1av.:- 1.Q33-_ rq33j_--25: i Hodonin _ Q_J _Q.: t- f :9.._: okres L- teckl..l. rl!è!1è.elk,!!_. HB : HavlickuvBrod Q _ Q_...Q ---l- _ Q.. n Q. ->- -i ; :mov u! : -t-+ ;- n_ ZR lld'árnadsázavu_n ==-=- j t!=-- r -g;- -g L VYVyskov ---l- 666 : : : ; 9 :_ : ZN Znojmo 4Q_! _Q. r' _n _'!Q9_ U J _ o Q_l - i- ol ' o SPRNG ANNEX l and 2007; automaticky vypocet strana 4 z 6

43 rok 2007 kampan Spring lornoucky kraj olozené krn2 okres letecky ruènë celkern JE Jesenik L llomouc 1- PV Prostêjov PR PFerov SU Sumperk okres letecky ruènë celkern , BR Bruntál FM F,ydek-Mistek K Karviná 347 i ; l NJ N0vYJicin P pava S strava-mêsto ,.; - okres letecky ruènë celkern KM KromêFiz UH Uherské Hradistê VS. Vsetin ZL Zlin SPRNG ANNEX l and 2007; autornaticky vypocet strana 5 z 6

44 133 rok: 2007 J kampali Spring èeská republika hranice SRN hranice PL hranice SVK hraniceaut letecky rucnè celkem ! i o o r, pocet dávek na km2 pri leteckém kladeni [pocet ávek na km2 pri rucni kldni SPRNG ANNEX l and 2007; automaticky vypocet strana 6 z 6

45 rálni vakcinace lisek -ceny jsou bez DPH rok 2007 kampan Spring okres Praha-mêsto cena za dávku cena za cena za cena za kladeni letecky kladeni rucnê uskladnêni l l cena za transdort soucet cena za cena za cena za cena za cena za soucet okres dávku kladen i leteckv kladeni rucnê uskladnêni transport BN Benesov l BE Beroun l KL Kladno K Kolin KH Kutná Hora ME Mêlnik MB Mladá Boleslav NB Nymburk PY Praha-vychod l PZ Praha-západ PB Pfibram RA Rakovnik l l l SPRNG ANNEX l and 2007,cena vakcinace stránka 1 z 6

46 cena za cena za cena za cena za cena za okres dávku kladeni leteckv kladeni rucnê uskladnêni transport CB Ceské Budêjovice D,DKc D,DKc D,DKc D,DKc CK Cesky Krumlov D,DKc D,DKc D,DKc D,DKc JH Jindrichuv Hradec D,DKc D,DKc D,DKc D,DKc P Pisek D,DKc D,DKc D,DKc D,DKc PT Prachatice D,DKei D,DKc D,DKc D,DKc ST Strakonice D,DKei D,DKc D,DKei D,DKc TA Tábor D,DKc D,DKc D,DKc D,DKc D,DKei D,DKc D,DKei 0,00 Kei D,DKc D,DKc soueet D KT PJ PM PS Ra TC okres Domazlice Klatovy Plzen-jih Plzen-mêsto Plzen-sever Rokycany Tachov cena za dávku D,DKc D,DKc D,DKc D,DKc D,DKc D,DKc cena za kladeni letecky 0,00 Kei D,DKc D,DKc cena za cena za cena za. t kladen i rucnê ;!_é!9!!.!1l.!.rq.e9_1'! : --% :::::::::::::::::::::::r::::::::::::::::::::::: ::::::::::::::Q9:q: r g lhlfi cena za. cena za cena za cena za cena za soueet okres dávku kladeni leteckv kladeni rucnê uskladnêni transport Cheb D,DKc) 99_q_ Karlovy Vary D,DKei D,DKc 99_q_ Sokolov 0,00 Kei D,DKc D,DKc D,DKei D,DKc D,DKc) D,DKc SPRNG ANNEX l and 2007,cena vakcinace stránka 2 z 6

47 1 DC CV LT LN M TP UL okres Dêcfn Chomutov Litomérice Louny Most Teplice Ûsti nad Labem -,-.-ce-na- za çfávku ,00 Kei 0,00 Kei 0,00 Kei 0,00 Kei cena za kladenfletecky ,00 Kei 0,00 Kei 0,00 Kei,Kcl ,00 Kei cena za kladeni rucnê 1 0,00 Kei 0,00 Kei 0,00 Kei 0,00 Kei 0,00 Kei 0,00 Kei 0,00 Kei 0,00 Kei cena za uskladnênf cena za transoort souœt.:9_9.!9_q_'5.. 9.!9_q_'5.. 9.!9_q_'5 9.!9_q_'5 9.!9_q_'5.. 9.!9_q_'5.. 0,00 Kei ,00Kc cena za cena za cena za cena za cena za soueet dávku kladenf letecky kladeni rucnê uskladnêni transport ?_.:_9.!9_q_' ,00 Kei Ê..:_q9.!9_q_' ,00Kc ,00 Kei ,00 Kc ä5(fÖ-Kt ,00 Kc 0,00 Kei 0,00 Kei ,00 Kc l 1 cena za cena za cenaza cena za cena za okres dávku kladenf leteckv kladenf rucnê uskladnênf transport 1 soueet [ HK Hradec Králové 0,00 Kei 9.!9_q_'5 JC Jicfn ,00 Kc.:_q?.!9_q_'5 NA Náchod ?_.:_9.!9_q_'5 RK Rychnov nad Knêznou !!.:_9.!9_q_'5 TU Trutnov ,00 Kc ,00 Kc SPRNG ANNEX l and 2007,cena vakcinace stránka 3 z 6

48 r--0. im. cena za cena za cena za cena za cena za, soucet okres dávku kladeni letecky kladeni rucnê uskladnêni transport CR Chrudim PU Pardubice SY Svitavy U Ûsti nad rlici ,00Kc ,00Kc l l l l cena za cena za cena za cena za cena za okres dávku kladeni leteckv kladeni rucnê uskladnêni transport soucet Havlickuv Brod Q9 Jihlava Q9 Pelhì'imov Tì'ebic 99q 7dár nad Sázavou l l cena za cena za cena za cena za cena za okres dávku kladeni leteckv kladeni rucnê uskladnêni transport soucet BK Blansko l Q9 BM Brno-mêsto l Q9 B Brno-venkov z.:9_Q9 '5.. BV Bì'eclav ,00 Kc.:_9 H Hodonin i?.:_q9 VY Vyskov ?.:_Q9 ZN Znojmo ,00 Kc ,00 Kc SPRNG ANNEX l and 2007,cena vakcinace stránka 4 z 6

49 ,, i cena za cena za cena za cena za cena za soucet okres dávku kladeni leteckv kladeni rucnê ' uskladnêni transport Jesenfk l l.:_q.!9 '5E lomouc l l Q.!9 '5E Prostêjov 39.00l 13.80l?.:_Q.!9 '5E prerov l D,DKc umperk ,00 Kcl D,DKc j25ö-ö-K ,00 Kcl D,D Kc D,DKcl D,D Kc ,00 Kc, cena za cena za cena-za cena za cena za -- okres dávku kladeni leteckv kladeni rucnê uskladnêni transport Bruntál ,00 Kc l T!-_?.:_?.!9_q_'5E Frydek-Mistek ,00 Kc l?.:_?.!9 '5E Karviná ,00 Kc l.:?_?.!9 '5E Navy Jicin ,00 Kc l.:?_?.!9 'SE pava l?.q.:q.!9 '5E strava-mêsto D,D Kc 6.65l l D,DKc 'o' o cena za cena za cena za cena za cena za soucet okres dávku kladeni leteckv kladeni rucnê uskladnêni transport KM Kromêriz l D,DKc.:?.QQ.!9 '5E UH Uherské Hradistê l ,00 Kc l ,00 Kc VS Vsetin l ,00 Kc D,D Kc 3@9K. ZL Zlin l ,00 Kc ,00 Kc l ,00 Kc D,DKcl D,DKcl ,00 Kc soucet...',.,2.".'.e SPRNG ANNEX l and 2007,cena vakcinace stránka 5 z 6

50 Ceská republika hranice SRN hranice PL hranice SVK hranice AUT CELKEM v Kc CELKEM v EUR ,00 Kë ,00 Kë ,00 Kë ,00 Kë 0,00 Kë ,00 Kë 6.900,00 Kë ,00 Kë ,00 Kë 0,00 Kë ,00 Kc , , ,00 Kë 0,00 Kë 0,00 Kë 0,00 Kë 0,00 Kë[ ,33 0,00 Kë ,00Kë 0,00 Kë,??_qg9_q_E!?g9_q_E! g9_q_e 0,00 Kë 0,00 Kë ,00 Kë 0, ,63 SPRNG ANNEX l and 2007,cena vakcinace stránka 6 z 6

51 Rabies ral Vaccination of Foxes Number of baits distributed by hands - 18 baits/km2, Number of baits distributed by airplane baits/km2. Yearl 2007 Campaign Spring BN BE KL K KH ME MB NB PY PZ PB RA district Benesov - Beroun Kladno Kolin Kutná Hora Melnik Mladá Boleslav Nymburk Praha-yYchod Praha-západ Pribram Rakovnik by airplane by hands total tl.''-:11h-...., district l=yairplane ëeské Budêjovice CK ëesky Krumlov JH P PT ST TA Jindrichuv Hradec Pisek Prachatice Strakonice Tábor 0-'_ - by hands total []. Number of baits..fel:... l.\. -{è- f --t-- Stránka 1 ANNEX l vaccination

52 Year' 2007 Campaign Spring -T district _l by!lirpj by_ha c!s_ J tp -=ai. L H i-=-tr g! gh =--:--=g +-1 : :$ci.,;,r---l-h-u-t-- gln--=-u- _---Q -=%- g TC Tachov Q.-1- Q.. : Q-L oi, g =f Yai.r:plan_L.!!Y han1 total -=ai. ---l Q _ i.--=- g--;--- g f--n=i-i---=--r---ff district Yairplane by hands total.. Dêéin : CV Chomutov l l =---r LT Utomêì'ice - LN Louny ïi:be--- PJ Plzen-jih, : u u_ n : -n ---r T -t ---- PM Plzen-mêsto '! -j-, - h- '912; n î 22:40 n n - _ o ---=E= -=J.2J Ï=J Stránka2 ANNEX l vaccination

53 Yearl 2007 Campaign Spring gion of Liberec Area in km2 - Number JC Jicfn district by airplane by hands total Ri'iJlf@ Cl èeská Lipa T of baits district byairplane by hands total a:wrmsm. tl".ß.t.ç MGtdftr HK Hradec Králové T NA Náchod RK Rychnov nad Knêznou l o: TU Trutnov Region of Pardubice Area in k district byairplane by hands tlal PEtwtlfiP'"..,.l".J:JWe-t'l'oMJ,...[:nt?.m CR Chrudim PU Pardubice Sy Svitavy ua UsU nad rtici Stránka 3 ANNEX l vaccination

54 Year 2007 Campaign Spring.. d;s";ct. by a;rplane--,--"y handsj total -- -1?:td-- n-l: -Hi---l> -J-- t-i' t, i dár nad Sázavou -j. -.o-i- : r--u-- H fi _H_ H _uo BK BM B BV H VY ZN district Blansko Brno-mesto Brno-venkov Bfeclav Hodonin ---- Vyskov Znojmo ,- L =_= _ ! EYailJlL!!y hands L total_ _- t- -- t j -= _- -Q_= L i l r Ö district Jesenik l lomouc - PV Prostèjov :- _._----- _L':ferov SU. i Sumperk-- t»l.airplane J! _ f -_ i, 120-0:--- e l'.-:.t':... Number of baits.au o,..j: _r l : iooft: t ; ' 'J" Stránka 4 ANNEX l vaccination

55 Yearl 2007 k Campign Spring ].. BR FM K NJ P S district Bruntál Fr)tdek-Mistek Karviná N0vY Jicin pava strava-mêsto byairplane by hands total _ o KM UH VS ZL district Kromêì'iz Uherské Hradistê Vsetin Zlin byairplane by hands total Wil'sJfftlfiW- "]fi number of baits per km2 by airplane number of aits per km2 by hands Stránka 5 ANNEX l vaccination

56 Rabies ral Vaccination of Foxes Number of baits distributed by hands - 18 baits/km2, Number of baits distributed by airplane baits/km2, Price per one bait - 13,00 Czk (without,19%vat), Price for la in km2 b air lane - 115,- Czk, Price for la in km2 b hands - 50,-Czk. Prices for la in are without tax 19% VAT t Exchange rate EUR - 28,50 Czk. Yearl 2007 Campaign Spring price for l lv tjl'''',,", total district baits Praha-mêsto " 0,00 Kèl 0,00 Kè ==:J price for price for ì price for price for price for district baits lavina bv airplane lavina bv hands storage transport total BN Benesov 0,00 Kei 0,00 Kei 0,00 Kè 0,00 Kè 0,00 Kèl BE Bereun 0,00 Kèl 0,00 Kèl 0,00 Kèl 0,00 Kè 0,00 Kè 0,00 Kè KL Kladno 0,00 Kèl 0,00 Kèl 0,00 Kè 0,00 Kè 0,00 Kèl 0,00 Kè K Kolin 0,00 Kei 0,00 Kè 0,00 Kè 0,00 Kè 0,00 Kè 0,00 Kè KH Kutná Hora 0,00 Kei 0,00 Kèl 0,00 Kè 0,00 Kè 0,00 Kè 0,00 Kè ME Mêlnrk 0,00 Kèl 0,00 Kè 0,00 Kè 0,00 Kè 0,00 Kè 0,00 Kè MB Mladá Boleslav ,00 Kei ,00 Kè 0,00 Kè 0,00 Kè 0,00 Kè ,00 Kè NB Nymburk 0,00 Kei 0,00 Kè 0,00 Kè 0,00 Kè 0,00 Kè PY Praha-yYchod 0,00 Kei 0,00 Kè 0,00 Kè 0,00 Kè 0,00 Kè PZ Praha-západ 0,00 Kèl 0,00 Kèl 0,00 Kè 0,00 Kè 0,00 Kè 0,00 Kè PB PFlbram 0,00 Kèl 0,00 Kèl 0,00 Kè 0,00 Kè 0,00 Kè 0,00 Kè RA Rakovnrk 0,00 Kèl 0,00 Kè '0,00 Kè 0,00 Kè 0,00 Kè 0,00 Kè ,00 Kèl ,00 Kèl 0,00 Kè 0,00 Kè 0,00 Kè ,00 Kè Stránka 1 AN N EX price

57 price for price for price for price.for price for total district baits laying by airplane laying bv hands storaqe transport CB Ceské Budêjovice CK Cesky Krumlov JH JindriehUv Hradec P Pisek PT Prachatice ST Strakonice TA Tábor 4. Region of Pizen price for price for price for price for price for total district baits lavinq bv airplane layinq by hands storage transport D Domazlice!lQ_ KT Klatovy!lQ_ PJ Plzerì-jih!lQ_ PM Plzerì-mêsto!lQ_ PS Plzerì-sever l l l!lq_ R Rokycany l l ,Q- TC Tachov 0, ëi,(5ö-t Kc.price for price for price for price for price for total district baits laying byairplane lavinq bv hands storaqe transport CH Cheb l ,Q- KV Karlovy Vary ,Q- S Sokolov l Stránka 2 ANNEX price

58 price for price for price for price for price for district baits layinq by airplane layinq by hands storaqe transport DC Dêcin ,00 Kei?.:QQ,QQ_!5. CV Chomutov 0,00 Kei Q,QQ_!5. LT Litomêì'ice Q,QQ_!5. LN Louny 0,00 Kei Q,QQ_!5. M Most Q,QQ_!5. TP Teplice Q,QQ_!5. UL Ûsti nad Labem 0,00 Kei ,00 Kei total " price for price for price for price for price for baits layinq by airplane lavina bv hands storaqe transport total " ,00 Kei 1.Q,QQ_! ,00 Kei QQ,QQ_! ,00 Kc 0,00 Kei ä5",oöï<t ,00 Kc 0,00 Kei ,00 Kc,- price for price for price for price for price for district baits lavina bv airplane lavina by hands storage transport total HK Hradec Králové Q,QQ_!5. JC Jicin ,00 Kc i:_q,qq_!5. NA Náçhod i:q,qq_!5. RK Rychnov nad Knêznou :Q,QQ_!5. TU Trutnov ,00 Kc ,00 Kc Stránka 3 ANNEX price

59 'ìt:.r:mr. price for price for price for price for price for district baits layinq by airplane layinq by hands storaqe transport tota CR Chrudim 0,00 Kë 0,00 Kë 0,00 Kë 0,00 Kë 0,00 Kë 0,00 Kë PU Pardubice 0,00 Kë 0,00 Kë 0,00 Kë 0,00 Kë 0,00 Kë 0,00 Kë SY Svitavy 0,00 Kë 0,00 Kë 0,00 Kë 0,00 Kë 0,00 Kë 0,00 Kë U Usti nad rlici ,00 Kë ,00 Kë 0,00 Kë 0,00 Kë 0,00 Kë ,00 Kë ,00 Kë ,00 Kë 0,00 Kë 0,00 Kë 0,00 Kë ,00 Kë. Region Vysocina price for price for price for price for price for district baits layinq by airplane laying by hands storage transport tota HB Havliëkuv Brod 0,00 Kë 0,00 Kë 0,00 Kë 0,00 Kë 0,00 Kë Q'Qg_ J Jihlava 0,00 Kë 0,00 Kë 0,00 Kë 0,00 Kë 0,00 Kë 0,00 Kë,Ö-Kt PE Pelhrimov 0,00 Kë 0,00 Kël 0,00 Kë 0,00 Kë 0,00 Kë --,Ö-Kt TR Trebië 0,00 Kë 0,00 Kë 0,00 Kë 0,00 Kë 0,00 Kë ZR 'Ldár nad Sázavou 0,00 Kë 0,00 Kë 0,00 Kë 0,00 Kë 0,00 Kë,Ö-Kt 0,00 Kë 0,00 Kë 0,00 Kë 0,00 ë 0,00 Kë 0,00 Kë price for price for price for price for price for district baits laying by airplane laying by hands storage transport total BK Blansko 0,00 Kë 0,00 Kë 0,00 Kë 0,00 Kë 0,00 Kë Qg- BM Brno-mèsto 0,00 Kë 0,00 Kë 0,00 Kë 0,00 Kë 0,00 Kë Qg- B Brno-venkov ,00 Kë ,00 Kë 0,00 Kë 0,00 Kë 0,00 Kë ??.:QgQg- BV Breclav ,00 Kë ,00 Kë 0,00 Kë 0,00 Kë 0,00 Kë ,00 Kë H Hodonin ,00 Kë ,00 Kë 0,00 Kë 0,00 Kë 0,00 Kë _:::::::.gq,qg:k VY Vyskov ,00 Kë ,00 Kë 0,00 Kë 0,00 Kë 0,00 Kë f.i.q,qg_ ZN Znojmo ,00 Kë ,00 Kë 0,00 Kë 0,00 Kë 0,00 Kë ,00 Kë ,00 Kë ,00 Kë 0,00 Kë 0,00 Kë 0,00 Kë ,00 Kë Stránka 4 ANNEX price

60 ,!, price for price for, price for price for price for.1 district baits laying by airplane. laying by hands storage transport Jesenik l 82.80l l l l !.QQ,QQ- lomouc l l l l Q,QQ- Prostêjov 39.00l 13.80l l l ?_.QQ,QQ- Pi'erov l l l l ?.Q,QQ- umperk l ,00 Kcl l l ,00 Kc l ,00 Kcl l l ,00 Kc total l l' price for price for price for price for price for district baits lavinq bv airplane lavinq bv hands storage transport total BR Bruntál ,00 Kcl l _.???:?.'QQ- FM Frydek-Mistek ,00 Kcl l :?.'QQ- K Karviná ,00 Kc ?.:Q?..'QQ- NJ N0vY Jicin ,00 Kc ?..?Q?..'QQ- P pava Q:Q,QQ- S strava-mêsto ,00 Kè ,00 Kèl l price for price for price for district baits hands stora e trans ort total KM Kromêi'fi l l i..?.qq,qq_ UH Uherské Hradistê )_.Q-'QQ- VS Vsetin l :-'QQ- ZL Zlin ,00 Kc ,00 Kei l ,00 Kc "..t Stránka 5 ANNEX price

61 Czech Republic border BRD border PL bordersvk borderaut l.,,..,'_s' ,00 Kei ,00 Kc ,00 Kei l ,00 Kc f...qq,qq_!.:??.._q,qq-!.:_1?.:q,qq_ Stránka 6 ANNEX price

62 # - rucnè BN Benesov BE Beraun BK Blansko BM Brno-mèsto B Brno-venkov BR Bruntál BV Breclav Cl Ceská Upa mceské Budèjovice CK Cesky Krumlov 1 DC Dèèin i D Domazlice FM FrYdek-Mistek HB Havlièkuv Brad H Hodonin HK Hradec Králové CH Cheb CV Chomutov CR Chrudim JN Jablonec nad Nisou JE Jesenik JC Jièin J Jihlava JH Jindrichuv Hradec KV. KarlovyVary K Karviná Kl Kladno J KT Klatovy. l_ a o K Kolin KM Kromèriz KH Kutná Hora 12/06/2006 rálni vakcinace lisek.. rok 2007 kampan Autumn AUTUMN ANNEX l and 2007; vkládaci èást strana 1 z 8

63 Plzen-mesto, /06/2006 rálni vakcinace isek rok i 2007 kampari Autumn J:.!!Jberec LT Litomerice ß u u_em_ i : : i : iili Boleslav --_26J 1.05 i : -l t- j M : Most! +:cin - n1ff---i-1 r=--- : --- -i : +:u.r==-=:ee' i _l pava 1 S - strava-rnèsto.. _ PU Pardubice &::J! =1-: : - r----: : 1! 1.144! J.7Q_ l : ij : PE Pelhrimov _ - _ - L_-==-o 0-2=- - -o-t -l- + - L =_:[] :}i:=-jih 1_ 1 :_ - : PM! PS Plzen-sever 1 : i> Q AB Praha-rnès!o + nn : ft- J :: :ad-!-td-f- " n L_ 1- _ -. J - :èe -= :.. n_n..l" 12"';' n;:-=-, 3.00 Prerov! 884 _u 2J.700 Q _.Lribr..J L _ l o -- AUTUMN ANNEX l and 2007; vkládaci cást strana 2 z 8

64 12/06/2006 rálni vakcinace lisek rok.. letecky RA Rakovnik kampan Autumn ruèné celkemo Ra Rokycany RK Ryehnov nad Knêinou SM Semily S Sokolov..... ST Strakonice r SY Svitavy SU Sumperk TA Tábor TC Taehov ' TP Teplice TU Trutnov TR Hebit UH Uherské Hradistê UL Usti nad Labem ua Usti nad arliei VS Vsetin VY Vyskov ZL Zlin *znojmo ZR Zdár nad Sázavou.Ceská republika hranice SRN hranice PL hranice SK hraniceaut.. CELKEM " AUTUMN ANNEX l and 2007; vkládaci tást strana 3 z 8

65 12/06/2006 rálni vakcinace lisek rok: 2007 kampali Autumn letecky rucne L celkem. pocet dávek na km2pr. leteckém kladeni : _25 _-. ; pocet dávek na km2pr. rucnim kladeni _ 18 AUTUMN ANNEX l and ; vkládaci Cást strana 4 z 8

66 12/06/2006 cena za dávku 1.00 Kei cena za 1 km21etecky 115,00 Kei cena za 1 km2 ruënê 50,00 Kei l kurs za 1 Euro 28,50 Ke/1 EUR! AUTUMN ANNEX l and 2007; vkládaci Cást strana 5 z 8

67 (') CD Dl lì! c. :1»" '< ;lç" s:::» C -l C s: z» z z m X Q) ::J a. N - < ;lç" Qi: a. Q) Q. e l. Cl - ::J Q) '> N (Xl... '> ì'3 '>

68 CD o CD e Ṇ N... co c: -ln iii ; '(3 co " -ca 32 >,:.: o N = " c: co x w zz «z ::> ::> «:J > -co " S eu c: C) CJ

69 l'd ;:, &1 o. ll. < "C }> C -l C s: z }> z z m X Q) ;:, - = \.) o o - <"iiï- - Q) Q. : eṉ ëi3 ::J Q) (Xl N (Xl... \.) -- o o o cr>

70 lorálni vakcinace lisek rok 2007 kampan Autumn okres letecky rucnê celkern BNl Benesov BE Beroun l KL Kladno. ' K Kolin KH Kutná Hora ME Mèlnik MB Mladá Boleslav NB Nymburk PY Praha-vYehod - PZ Praha-západ PB Pì'ibram RA Rakovnik AUTUMN ANNEX l and 2007; automatieky vypoéet strana 1 z 6

71 Jl_ rok: q9 _ J kampan Autumn okres CB Geské Budêjovice cktce_sy Krum JH! Jindì'ichuv Hradec -PfTPiSek--- PT Prachatice ST Strakonice TA. Tábor --i! ruënê 1_ celkem_ a 1- a ' a a a i a _- -_ - - o - -l---=- ---T a a a a a a a a - ' a a a a a a o o j i j a a! a, a ò+ al -0-: a a 1 0-: a a a. a a öu- T o. o a _ 9 _ a a a : ---j -- - _9 o _ o o i okres Domazlice KT : Klatovy PJ i Plzen-jih PM Plzeif-mêsto PS! Plzen-sever R Rokycany TC Tachov ruënë celkern a i a _ a a -+ a a a --==--ja a a a a a a -*+- [j a n---t-- -g +---%-t- - un _ ö: a 1_=9 _i a, :_ a a 1 J o a a a Ln a u u_. --r -,-- -- u Q : a a o o o o - _ a ' -=----.L a.-- --t a --.L t, o AUTUMN ANNEX l and 2aa7; automaticky vypocet strana 2 z 6

72 rok 2007 kampali Autumn okres letecky ruènè celkern DC Deein CV Chomutov lt Litomerice ln Louny M Most TP Teplice Ul Usti nad Labem okres letecky ruènè celkern Cl Ceská Lipa JN Jablonec nad Nisou lb Liberec SM Semily okres letecky t. ruènè celkern HK Hradec Králové JC Jiein NA Náchod RK Rychnov nad Kneinou TU Trutnov t AUTUMN ANNEX l and 2007; automatickyvypoèet strana 3 z 6

73 i 3.499! rok' 2007 kampan Autumn t okres.leteckï- CR : Chrudim o, PU Pardubice. sv Svitavy U Ustl nad 6 [ rucnê celkemo i i -! t - o,, : HB _ J PE TR ZR okres 1 le!.eï.. l ljen!._ Havlickûv Brod i - - ihlé! -_- _J?- = QuJ i =- Q -; Pelhrimov uu_ njl Q -o-- Hebic n : --- 'Zd'ár nad Sázavou _ g t g - : -T:--- L_ Ö- okres letecky! rucnê : oês9 _u_- _ -- - B Brno-venkov 400 _BVj Bfeclav- -l1ß33 H ' Hodonin t - VY -Vyskov 666 t ---o ZN Znojmo t o l _ celke"! : 01-- : 400-1J)öo' r = 25.Q i- J : : 400: r Q..-,-- -, t o - [ t L s-"QQ _800 o: t AUTUMN ANNEX l and 2007; automaticky vypocet strana 4 z 6

74 rok kampali Autumn okres letecky ruènê celkem Jesenik j T lomouc T Prostèjov Prerov umperk BR FM K D P S okres Bruntál FiYdek-Mistek Karviná N0vY Jicin pava strava-mèsto letecky ruènê celkem [ l j , H okres letecky ruènê celkem KM Kromèriz UH Uherské Hradistè VS Vsetin ZL Zlin AUTUMN ANNEX l and 2007; automaticky vypocet strana 5 z 6

75 2007 rok J kampan Autumn letec_rucnè celkem èeská republika : ; hranice SRN 60 l l l hranice PL i, 3.S hranice SVK l hranice AUT l l l pocet dávèk-km2 pri leteckém kladeni --.=J j.3ô _ --J pocet_c:láve.k na 2 pri rucnim kladeni -.L " AUTUMN ANNEX l and 2007; automaticky vypocet strana 6 z 6

76 rálni vakcinace lisek -ceny jsou bez DPH rok 2007 kampan Autumn cena za dávku cena za kladenf rucnê cena za uskladnênf soucet cena za. cena za cena za cena za cena za okres dávku kladenf leteckv kladen f rucnê uskladnênf transport soucet BN Benesov BE Beroun KL Kladno l K KoHn KH Kutná Hora ME Mêlnfk MB Mladá Boleslav NB Nymburk PY Praha-vYchod PZ Praha-západ PB PFfbram RA Rakovnfk ,00Kcl l l AUTUMN ANNEX l and 2007,cena vakcinace stránka 1 z 6

77 cena za cena za cena za cena za cena za okres dávku kladeni leteckv kladeni rucné uskladnéni transport soucet CB Ceské Budéjovice CK Cesky Krumlov l l JH JindFichuv Hradec l P Pisek PT Prachatice l ST Strakonice TA Tábor l D KT PJ PM PS R TC okres Domailice Klatovy Plzeri-jih Plzeri-mêsto Plzeri-sever Rokycany Tachov cena za dávku l cena za kladeni leteck cena za cena za l cena za c t kladeni ru ::[email protected]!1l tr.e_"! + : :::::::::::::::::::::::t:::::::::::::::::::::::[:::::::::::::::: 9!9_q_ 9!9_q_ [::::::::::::::::::::: ::::::::::::::::::::::: ::::::::::::::9:: Ö:Ö-Ö-Kt Ö:Ö"(fKt Ö:Ö-Ö-Kt cena za cena za cena za cena za cena za soucet okres dávku kladen i'eteckv kladeni rucné uskladnêni transport CH Cheb l 9!9_q_ KV Karlovy Vary l 9!9_q_ S Sokolov T! l AUTUMN ANNEX l and 2007,cena vakcinace stránka 2 z 6

78 '-- DC CV LT LN M TP UL okres Dêcin Chomutov Litomêfice Louny Most Teplice Ûsti nad Labem cena za. dávku l l l l l l l cena za cena za cena za., kladeni letecky kladeni rucnê uskladnêni ' l l l l l l l l l l l l j cena za transoort soü\cet }_?.:9_9.!9 '5 9.!9 '5 9.!9 '5 9.!9 '5 9.!9 '5 9.!9 '5 l cena za cena za cena za cena za cena za soucet dávku kladeni leteckv kladeni rucnê uskladnêni transport l l 92.00l l ,00 Kc l :f53:fä5ö-ö-kt ,00 Kc l ,00 Kc cena za cena za cena za cena za cena za soucet okres dávku kladeni leteckv kladeni rucnê uskladnêni transport HK Hradec Králové l- l 9.!9 '5 JC Jicin l ,00 Kcl l.:_.!9 '5 NA Náchod ?_.:_9.!9 '5 RK Rychnov nad Knêznou :_9.!9 '5 TU Trutnov ,00 Kc l ,00 Kc AUTUMN ANNEX l and ,cena vakcinace stránka 3 z 6

79 tr;';;rt cena za cenaza cena za cena za cena za okres dávku kladenr letecky kladenr rucnè uskladnèni transport soucet CR Chrudim PU Pardubice SY Svitavy U UsU nad rlici l l l cena za cena za cena za cena za soucet okres dávku kladeni letecky kladeni rucnè uskladnèni Havlickûv Brod l gl9_q_ Jihlava l 9l9_q_ Pelhrimov l 9l9_q_ Trebic l 9l9_q_ dár nad Sázavou l l l cena za cena za cena za cena za cena za okres dávku kladeni letecky kladeni rucnè uskladnèni transport BK Blansko 9l9_q_ BM Brno-mèsto 9l9_q_ B Brno-venkov z.;9_qgl9_q_ BV Breclav ,00 Kc?.;_?l9_q_ H Hodonin ?.;_q9l9_q_ VY Vyskov ?_.;_9l9_q_ ZN Znojmo ,00 Kc ,00 Kc soucet AUTUMN ANNEX l and 2007,cena vakcinace stránka 4 z 6

80 \ okres Jesenfk lomouc Prostêjov Pì'erov Sumperk cena za dávku l l 39.00l l ,00 Kei l cena za kladenf letecky 82.80l l ,00 Kei l ,00 Kcl ,00 Kcl j cena za kladenf rucnê l l l l l l cena za uskladnênf : l i 0,00Kci cena za transoort 0,00 Kei j j soueet _.;.QQ.!9 '5 Q.!9 '5?.;_Q.!9 '5 _.;?Q.!9 ' ,00 Kc ,00 Kc cena za cena za cena za cena za cena za okres dávku kladenf letecky kladenf rucnê uskladnênf transport soucet BR Bruntál ,00 Kc?_?.;_.!9 '5 FM FrYdek-Mfstek ,00 Kc.;_.!9 '5 K Karviná ,00 Kc.;?.Q.!9 '5 NJ NavY Jicfn ,00 Kc.;?.Q.!9 '5 P pava S strava-mêsto fä-5Ö(f6-Kt l cena za cena za cena za okres dávku kladenf letecky kladenf rucnê uskladnênf transport l cena za cena za KM Kromêì'fz l.;?_Q.!9 '5 UH Uherské Hradistê ,00 Kcl 1.;_.!9 '5 VS Vsetfn ,00Kcl...;_.!9 '5 ZL Zlfn ,00Kc ,00KcT ,00 Kc ,00 Kcl l ,00 Kc soucet. AUTUMN ANNEX l and 2007,cena vakcinace stránka 5 z 6

81 Ceská republika hranice SRN hranice PL hranice SVK hranice AUT CELKEM v Kc CELKEM v EUR ,00 Kei ,00 Kei ,00 Kei ,00 Kei 0,00 Kei ,00 Kei ,00 Kc , , ,00 Kei ,33 l ,00 Kc,??_q99_q_ 1.z?99_q_ 1_?99_q_' ,00 :t1'iti Kc. 0, ,63 AUTUMN ANNEX l and 2007,cena vakcinace stránka 6 z 6

82 Rabies ral Vaccination of Foxes Number of baits distributed by hands - 18 baits/km2, Number of baits distributed by airplane baits/km2. Yearl 2007 Campaign Autumn pital city Prague Area in km2 by hands total district byairplane by :itmmù- M@ÙìÇ tijltôtil@& m! BN Benesov - BE Beroun KL Kladno K Kolin KH Kutná Hora ME Mëlnik MB Mladá Boleslav NB Nymburk PY Praha-vYchod PZ Praha-západ PB Pì'lbram RA Rakovn k district by airplane by hand; total..- CB Ceské Budëjovice CK Cesky Krumlov ol - JH Jindì'ichuv Hradec P Pisek PT Prachatice ST Strakonice TA Tábor - Stránka 1 ANNEX l vaccination

83 Year! J Campaign Autumn 2007 district D T Domaziice KT Klatovy PJ Plzen-ji!! - i!r:e! _-hn]_ aj (f r u (W PM _+E'nT1!<?_ -gtl;er TC,Tachov _ [ ' --f i r--m l,......f'. i=. g g ---- DC CV LT LN M TP UL district Dêcin Chomutov Litomêfice --- Lou Most Teplice ÙSti nad Labem ---H-- o.r -ü-j -r j i+ by airplane i by hands total 912, 912 ' : Ö.',.....-o r o- ---o : i --- t =1-1=--. +=---=+- --t , t --- i l=---i -j , Stránka 2 ANNEX l vaccination

84 , " Yearl 2007.J Campaign Autumn gion of Liberec : district by airplane by hand; total '''J!f:.w. Cl Ceská Lipa JN Jablonec nad Nisou lb Liberec SM Semily Number of baits district by airplane hands total HK Hradec Králové JC Jicin NA Náchod RK Rychnov naa Knêznou TU Trutnov " 1J:.:mt.i..i.... Number of baits district by airplane by hands Chrudim Pardubice _vitavy Usti nad rlici Stránka 3 ANNEX l vaccination

85 -- 1 Year! 2007 Campaign Autumn "', t!iri l-y irp!ane by hands -L Havlickuv Brod! Jihlava _-- Pelhì'imov _ --of t--- - _ - Tì'ebic - f d'ár nad Sázavou Q _ - t- total ' dh lo o-r f== r l, Ü-j : Ö-j BK BM B BV H VY ZN district by airplane: by hands total --- 'fn-_-tn:_.oti--o1j..,0oj -n. _._ gln Znojmo : : ' JE l PV PR SU district - Jesenik - lomouc Prostejov Pì'erov Sumperk " - - Y _é!!rplane by hands L- _Jl...( 120 ' ï Ù; - ---o r - 1-4_ total t - 11.''-:11'' Number of baits. f:... 1'..l'.[:.J...-: Q.j Q._l l T i-- _Q Ö- -tl Bì'eclav 1.033: ! -- Hodonin E=1.0Q= = == =0- Yyskov_ 666 _1_ _[ -l- -0-; Q " _ ' Stránka 4 ANNEX l vaccination

86 Yearl 2007 Campaign Autumn oravia-silesian Region Area in km2 district by airplane by hands total BR Bruntál FM FrYdek-Mistek K Karviná NJ NavY Jicin P pava S strava-mësto district by airplane by hands total KM Kromêriz UH Uherské Hradistë VS Vsetin ZL Zlin " Area in km2 by hands 133 total l l.'. number of baits per km2 by airplane number of baits per km2 by hands Stránka 5 ANNEX l vaccination

87 Rabies ral Vaccination of Foxes Numberof baits distributedby hands- 18 baits/km2, Number of baits distributed by airplane baits/km2, Price per one bait -13,00 Czk (without 19%VAT), l'' Price for laying km2 by'airplane - 115,- Czk, Price for laying km2 by hands - 50,-Czk. Prices for laying are without tax (19% VAT), Exchange rate EUR - 28,50 Czk. Yearl 2007 Campaign Autumn district Praha-mêsto price for baits price for price for total hands l storaae transport 0,00 Kei l 0,00 Kei price for price for price for price for price for district baits layinq by airplane layinq by hands storaqe transport total. BN Benesov l 0,00 KCh BE Beroun l l KL Kladno 0,00 Kei l l K Kolin 0,00 Kei KH Kutná Hora 0,00 Kei ME Mêlnik 0,00 Kei MB Mladá Boleslav NB Nymburk 0,00 Kei 0,00 Kei PY Praha-vjchod 0,00 Kei 0,00 Kei PZ Praha-západ 0,00 Kei l PB pribram l 0,00 Kei RA Rakovnik l l Stránka 1 AN N EX price

88 price for price for price for pricefor price for district baits layinqby airplane laying by hands storage transport CB Ceské Budéjovice CK Cesky Krumlov JH Jindì'ichuvHradec P Pisek -PT Prachatice ST Strakonice TA Tábor price for price for price for pricefor price for total district baits layinq by airplane layinq by hands storaqe transport D Domazlice l l Q,QQ- KT Klatovy l Q,QQ- PJ Plzen-jih l Q,QQ- PM Plzen-mésto l Q,QQ- PS Plzen-sever l l l _PQQ- R Rokycany l l _PQQ- TC Tachov l l _PQQ- l l l total price for price for price for price for district baits la in b air lane hands stara e trans ort total CH Cheb l Q,QQ- KV Karlovy Vary l _PQQ- S Sokolov Stránka 2 ANNEX price

89 , price for price for' price for price for price for district r' ; baits lavina bv airolane 'avina bv hands storaqe transpórt DêCfn l l l?_"q9_'_q_ Chomutov l l l 9_'QQ_ Litomêì'ice l l 9_'QQ_ Louny l l l Q,QQ- Most l l l 9_'QQ_ Teplice l l Q,QQ- Ûstl nad Labem l l l l total.:!!...' ':; price for price for price for price for prtce for baits total lavina bv airolane lavina bv hands storaqe transport l 1i.Q,QQ_1 -"f i.qq,qq_ ,00 Kc ,Ö-Kt ,00 Kc ,00 Kc price for price for price for price for price for district baits lavina bv airolane lavina bv hands storage transport total HK Hradec Králové QQ- Je Jicfn ,00 Kc i-_q,_q_ NA Náchod ,00Kc. i.q,_q_ RK Rychnov nad Knêznou _.Q,-Q- TU Trutnov ,00 Kc ,00 Kc Stránka 3 AN N EX price

90 l:. :r:r.tr. price\for price for price f()r price for price for, district baits lavina bv airolane lavina bv hands storaqe transport total CR Chrudim PU Pardubice SV Svitavy ua UsU nad rlici l l l price for price for price for price for price for total district baits lavina bv airplane lavina bv hands storaqe transport HB Havlickuv Brod l ,Q- J Jihlava ,Q- PE Pelhi'imov ,Q- TR Tì'ebic ,Q- ZR Zdár nad Sázavou! price for price for price for price for price for total district baits lavinq bv airplane lavinq bv hands storage transport Blansko!l_Q_ Brno-mêsto ,Q- Brno-venkov ??.:Q,Q- Bi'eclav ,00 Kc ,00 Kc Hodonin H?.QQ,QQK Vyskov i.,q_ Znojmo ,00 Kc ,00 Kc Stránka 4 ANNEX price

91 i., price for district baits hands Jesenik l l l lomouc l 0,00 Kei rq,qgk Prostêjov 39.00l 0,00 Kei l ?.:.!!QQ,QQ- Pi'erov l 0,00 Kei l ?.:.?..Q,QQ- umperk l 0,00 Kei ,00 Kc l 0,00 Kei ,00 Kc price for price for price for price for price for total district baits laving bv airplane laving bv hands storage transport BR Bruntál ,00 Kc l l _.???:?.'QQ- FM Frydek-Mistek ,00 Kc l l ,00 Kcl.-'. K Karviná ,00 Kc l j.g,qgk NJ Novy Jicin ,00 Kcl l ?:.?Q?..'QQ- P pava l S strava-mêsto l Ci,(5ö-ß l '. t".... '.' ",:d'. ' " price for price for price for price for price for district baits lavina bv airolane lavina bv hands storage transport total KM Kromêi'ii ,00Kc ,00Kc i..?.qp.!qq_ UH Uherské Hradiätê l ,00 Kc J.:.!!QP.!QQ_ VS, Vsetin l ,00 Kc l l P.:?.'QQ-. ZL Zlin l ,00 Kc ,00 Kc l ,00 Kc 0,00 Kei l ,00 Kc total : Stránka 5 ANNEX price

92 '\ ,00 Kei D,DKei ,00 Kc ,00 Kei D,DKc D,DKc D,DKc 0,00 Kei 0,00 Kei 0,00 Kei D,DKei ,00 Kc??_.Q9.!QQ_.:??.:_9.!QQ._1?.:Q,_Q_ D,D Kc riti Stránka 6 ANNEX price

93 ,) PACKAGENSERT " /L YSVULPEN por. ad us. vet. Vaccine against rabies. ntended for oral immunization. Qualitative and quantitative composition Composition - dose = 1.8 ml: -Virus rabiei attenuatum SAD Berne min. 1.8 x 107TCDso- max. 1.8 x 108TCDso - MEM culture medium, stabilizing medium, bait material with tetracycline Pharmaceutical form The vaccine is intended for oral application and is contained in an aluminium-plastic blister, placed in the bait consisting of a special feeding mixture. mmunobiological characteristics Pharmacotherapeutical ATC.classification: - group: 97 Veterinaria immunopraeparata Q l5aa Veterinary vaccines The principle of the immunization effect of the vaccine is that, upon chewing the capsule containing the vaccination virus inside the bait, the vaccination virus escapes from the perforated capsule to the surface of the mucosae and tonsils in the oral cavity. n a vaccinated individual, the virus is recognized to be extraneous and activation of the immunity system starts and protective virus-neutralizing antibodies start creating. n this way, the immunized foxes and other species of target animals are protected against infection with the virulent field rabies virus and are unable to transmit rabies further. mmunity in the vaccinated animals lasts for at least one year. Protective effect against rabies may be accomplished only in uninfected foxes andraccoon dogs. The presence of colostral antibodies against rabies has a negative impact on the creation and protective immunity in fox cubs and raccoon dogs as well. The ingurgitation of more bait is harmless to the target species. The harmlessness of the vaccine has also been demonstrated in domestic animals (dogs, cats). The vaccination rabies virus may be distinguished from the field rabies virus by means of monoclonal antibodies../ mmunization in foxes and other species of target animals occurs after the ingurgitation of laid-out immunization baits containing the vaccination virus. mmunity in the immunized foxes and other target species forms after 21 days following ingurgitation of the bait containing the vaccination virus. Business denomination and registered office of the holder of the resolution on registratio"n Bioveta, a. s., Komenského vanovice na Hané Ceská republika Target species: Red fox (Vulpes vupes), raccoon dogs (Nyctereutes procyonoides). Withdrawal ndication: periods: Not intended for food-producing animals. To be used for the prophylactic vaccination of feral foxes and raccoon dogs against rabies. ""Contraindication: The vaccine is not to be used for the immunization of domestic animals.

94 ')... Special advice concerning safe use of the preparation Until laid out, either manually or by aircraft, the vaccine must not be exposed to temperatures exceeding 15 oe (it is to be distributed in thermo-cases or packed in heat-insulating material so that the vaccine is sufficiently protected against the effect of excessive external temperatures). f laid out manually in a field, the bait is to be tipped out of the cardboard box carefully. The bait is to be laid in spots that are protected from direct sunlight. After being laid out, the bait shall be covered with natural material (leaves, grass, soil etc.) to be protected from the sun. To prevent the hands from coming into direct contact with the bait, it is necessary to use protective gloves when laying out the bait in order to avoid the effects of human scent on the bait. The gloves are disposable (intended for the unloading of 1 carton = 20 bait items). During the winter, at temperatures below oe, the bait is not to be laid open in a field. After perforating the blister, the deep-frozen vaccine contained inside the bait may not come out, reach the mucosae of the oral cavity and thus immunize the animal. Two weeks after laying the bait, the free movement of dogs and cats should be avoided within the vaccination zone as these animls epresent competitors for the bait. The areas in which vaccination is performed are to be duly marked and the respective inhabitants must be informed in an adequate manner. The vaccine contains attenuated vaccination rabies virus. f the vaccine (the content of the aluminiumplastic blister deposited in the bait) comes into contact with human eyes, mouth, nose or broken skin, contact a physician immediately. n this case, medical procedures are conducted pursuant to the guidelines in force, dealing with exposure to the rabies virus. f the hands or other uncovered body parts become stained with the vaccine, the affected spot is to be duly washed with soap and water. The. bait containing the vaccine must be stored' out of the reach of unauthorized persons, especially children! nteraction with other veterinary preparations and other forms of interaction Unknown so far. Dosage and method of administration The immunization of the target species forms after the ingurgitation of the laid-out bait containing the vaccination virus. The vaccine is laid twice a year, namely in April or May and September or ctober. Usually, 15 to 20 baits or more are laid out for each square meter, depending on the epizootologic situation and population density, especially the fox population density. The baits with the vaccine have to be laid out throughout the entire area of the given tehitory. f an airplane or a helicopter is used, the flight lines must be adjusted to reach equal dispersion. For example, the baits are dropped every 250 meters, so the individual lines must also be at a distance of 250 meters from each other. n the event of necessity provoked by a contagious situation in the given area, spring or autun revaccination is recommended. t is also recommended to lay bait in a limited area in the summer or to lay the bait close to occupied fox dens in an amount of 10 baits per den. An aerial form of bait distribution is recommended in free areas with a low population density. n areas which are densely populated, it is preferable to distribute the bait manually. Vaccination of the respective territory is to be performed for two consecutive years, but at least for three years after the last occurrence of rabies in the respective territory. The vaccine mayalso be used for a rabies-free territory in order to form a protective zone. The width of such a zone entrenching on terdtory with rabies occurrence should not be smaller than 50 kilometers. Airplane distribution ofbait The bait is laid out in conformity with the previously prepared flight maps along the previously determined flight lines. The Satellite Navigation System (GPS) may be applied for orientation and correct distribution. Undesirable effects: Unknown

95 ' " ' \ Type of wrapping and size of package a) Manual distribution The vaccine (aluminium-plastic blister containing the vaccination virus inside ti feeding mixture bait) is packaged in a cardboard box with a fixative grid per 20 baits. The collated package in a carton contains 30 x 20 doses (i.e. 600 doses). b) Airplane distribution The vaccine (aluminium-plastic blister containing the vaccination virus inside a feeding mixture bait) is packaged: -. per 40 doses that are freely deposited in layers spaced with tapaten in a heat-insulated carton. The collated package in a carton contains lx 40 doses.. - freely in polyethylene bags per 350 doses in a heat-insulated carton. The collated package in a carton contains 2 x 350 doses. A package insert is attached with each package. Method of storage and reference to the indication on termination of applicability, stated on the package, advise of the prohibition to use the preparation after the lapse of the applicability period Store at a temperature of -20 and bellow. The preparation must not be used after the lapse of the applicability period, indicated on the package, Special measures concerning disposal of the unused veterinary preparation or of the waste originating from this preparation The packages and any remainders of the preparation are to be disposed of pursuant to the regulations in force. Date -of the last revision of the text, "

96 l 'l.. $ STATE VETERNARY ADMNSTRATN Czech Republic Slezská 7, PRAHA 2 Tel.: Fax.: Your letter did Your reference ur reference : 2006 / 808 / VET Attachement File handled by Department Phone Fax Dr. Satrán, Dr. Fejfarová Department of Animal Health and Welfare European Commission Health and Consumer Protection, Directorate-General Paola Testori-Coggi F013/56 B-l049 Brussels Belgium Prague Re: Additional information on animal disease and zoonoses eradication, control and monitoring programmes submitted for co-financing for Dear Paola Testori-Coggi, Enclosed please find additional information to the animal disease and zoonoses eradication, control and monitoring programmes submitted for co-financing for The electronic version of additional information to the programme was submitted to address: SANC- [email protected]. Yours sincerely, MVDr. Milan Malena, Ph.D. Director General (CVa)

97 Rabies Estimation ofwildife population The estimation of the fox's population you can find enclosure in the table according to Commission Decision 2004/450/EC. The hunting bag was considered as standard method of estimation. Enclosure: Table Salmonella Number of positive breeding flocks (Elite, Grandparents, Parents) for Salmonella sp. during period Year Number of positive breeding holdings for Salmonella sp Estimation of number of breeding establishmentslflocks covered by the control programme in 2007 Egg production line Meat production line n total Number ofbreeding holdings covered by the 9 control programme Number ofbreeding flocks covered by the control programme, Summary of measures under the programme (specify first year objectives) The measures taken under the programme in 2007 are: Testing Slaughter of positive animals Killing of positive animals Vaccination Treatment Disposal of product 1

98 Surveillance system on Salmonella in feedingstuffs implemented in holdings covered by the control programme. The State Veterinary Administration of the Czech Republic and the Central nstitute for Supervising and Testing in Agriculture perform checks of feedingstuffs in the territo Qf the Czech Republic. According to the Act No. 91/1996 on feedingstuffs, as amended, a Good Manufacturing Practice for feedingstuffs intended for farm animals is supervised by the Central nstitute for Supervising and Testing in Agriculture. Feedingstuffs intended for farm animals are examined for Salmonella by the State Veterinary administration of the Czech Republic through the competent Regional Veterinary administration in accordance with Regulation (EC) No. 1774/2002. The official veterinarians take samples of feedingstuffs: 1. in compound feedinstuffs production plants; final compound feedinstuffs are randomly sampled according to the volume of production before dispatch, and 2. in poultry holdings. 2

99 Data on the epidemiological evolution during the last (five) years and targets on testing of breeding flocks: (laid down by Commission Decision 2004/4S0/EC - Annex n. tiand 7. Data on evolution of the disease in the whole territory ofthe Czech Republic Year: Animal species: Gallus gallus - breeding flocks Disease/infection: SALMNELLA Year Type of holdings Total number of holdings Total number of animals Total number Total number Number of holdings of animas Number of Number of positive underthe under the holding holdings holdings programme programme depopula checked (al) (a2) (aj) (a3) (a l Number of flocks depopulated in last 5 years: Number of animals slaughtered/destroyed in last 5 years: We estimate to depopulate two flocks with animals in

100 Targets on vaccination Vaccine( s) and vaccination scheme SALMNELLA ENTERTDS Disease: SALMNELLA Animal species: Gallus gallus - breeding flocks Region Total number of Total number of holdings in animals in Numberof Numberof Numberof Number of doses holdingssin holdings animals of vaccine vaccination vaccination vaccination expected to be expected to be expected to be programme programme programme vaccinated vaccinated administered CZ Details of sampling framework pursuant to controls at the initiative of the operator and sampling implemented in the frame the official controls. Frequency of sampling in breeding poultry flocks: All samplings in breeding poultry flocks are compulsory and will be refunded from the state budget. The sequence of taking Sampling taken on the initiative of the fficial sampling n samples operator breeding poultry flocks (See definition for appropriate letter bellow) a) x b) x e) x c) x d) 14 times x (each 2 weeks) f) x g) x h) x Sampling taken on the initiative of the operator a) day-old chicks: 10 swabs from internal surfaces of boxes or crates used for transportation of the chicks. A sample from the entire consignment of chicks S concerned that is, if appropriate, combined to form a pooled sample; 4

101 b) carcasses of chicks found after arrival of a veterinarian (acceptance of chicks). A sample from the entire consignment of chicks (at most 60 birds) is concerned that is, if appropriate, combined to form a pooled sample; c) 2 weeks prior to laying phase - taking of pooled faecal sample; d) each 2 weeks during the laying phase - taking of pooled faecal sample. fficial sampling in breeding poultry flocks: e) in age of 4 weeks - pooled faecal sample; f) in 4 weeks from the start of laying phase - pooled faecal sample; g) after 20 weeks of laying phase - pooled faecal sample; h) 4 weeks prior to the end of layingphase - pooled sample. Testing methods used by laboratories under the control programme (official sampling + sampling taken on the initiative of the operator. Testing methods used by laboratories under the control programme for official sampling and sampling taken on the initiative of the operator are identical. Sampling must be carried out in accordance with minimum sampling requirements as laid down in Regulation (EC) No. 2160/2003 of the European Parliament and the Council and Commission Regulation (EC) No. 1003/2005. Diagnosis within this National Programme shall be carried out at the SV Prague - NRL for salmonellae, at the SV Jihlava and the SV lomouc. Laboratory examination comprising detection of Salmonella spp. shall be carried out in accordance with the method recommended by the Community RL in Bilthoven, Nethrlands. The method is a modification of S 6579 (2002), where a semi-solid medium (MSRV) is used as the single selective enrichment medium. Serotyping shall be carried out in at least one isolate from each positive sample, following the Kaufmann- White scheme. Fagotyping shall be carried out in accordance with the HPA Colindale, London. 5

102 Examination of at least one sample for inhibition substances shall be carried out within the framework of bacteriological examination of organs from official samples taken for the confirmation of test results Lifting of restrictions: All restrictions are lifted if all measures laid down in the emergency veterinary measures are fulfilled and if the final disinfection is carried out. The State Veterinary Administration of the Czech Republic confirms that:. the aim of the programme is to reduce the prevalence of the 5 Salmonella serovars referred to in point "objectives" - to a levelof 1% or lower by 31 December 2009,. the use of antimicrobials (in exceptional cases) is subject to authorizatiorl anq supervision of the competent authority and is based, wherever possible, on the results of bacteriological sampling and of susceptibility testing. The use of antimicrobials shall be carried out in accordance to Commission Decision No. 1177/2006,. live salmonella vaccines for which the manufacturer does not provide an appropriate method to distinguish bacteriologically wild-type strains of salmonella from vaccine strains are prohibited from use in the framework of national salmonella control programmes, documents accompanying the sample sent to laboratories under the control programme indicates whether the samples are derived from a vaccinated flock and the y'pe of vaccine used, an empty period is required after cleaning and disinfection. The period is required on the bases of technological procedure and takes at least for period of 14 days, feedingstuffs are restricted in case of confirmation, the conditions for registration of holdings are already applied. MTD Ustrasice registers the holdings and gives the holdings the registration numbers. A negative bacteriological result is not required before restocking. 6

103 The other measures which are foreseen to achieve the programme's objective are: The requirements of sanitary measures and biosecurity measures are carried out by keeping of technological procedure by the establishments which provide one-day old parent's chickens. The costs of tests were calculated for all samples which are compulsory " ' in the frame of programme. All samples are considered as official samples and it is foreseen that all samples will be refunded from the state budget. We would like to supplement our Programme for the Monitoring and Control of Salmonella spp in poultry breeding flocks for vaccination against Salmonella enteritidis. The vaccination shah be carried out in accordance with Commission Decision 1177/2006. t is expectd hat in total 322 flocks with animals will be vaccinated against Salmonella enteritidis. Live salmonella vaccine will be used for vaccination and inactivated vaccine will be used for revaccination. The estimation of total amount of the costs for vaccination is Czk. The following vaccines will be covered under the programme: Live vaccine nactivated vaccine Name of vaccine Manufacturer Name of vaccine Manufacturer NBlUS SG 9R ntervet, NBlLlS SALENVAC ntervet GALUVAC SE Merial NBlLlS SALENVACT ntervet TAD SALMNELLA VAC Lohman E GALLMUNESE Merial PULVAC SE Fort Dodge CEVAC SETK Fatro 7

104 Avian influenza The number of tests to be performed has been carefully formulated and numbers of tests have been revised. Fore number of tests for confirmation we estimated prevalence 10%. Tèsting will be carrying out in accordance with Diagnostic manual. Measures eligible for co-financing - Poultry Methods of laboratory Number tests performed per Cost (VAT excluded) analysis method Serological pre-screening ELSA (DEXX) ,- ezk (unitary cost 110,- ezk) ' Haemaggl utination- inhibiton- HsN test (unitary cost 110,- ezk) ,- ezk (H) for H5/H7 H7N (unitary cost 110,- ezk) ,- ezk HsN ,- ezk (unitary cost 110,- ezk) H7NJ ,- ezk (unitary cost 110,- ezk) per (unitary cost 900,- ezk) ,- ezk Virus isolation test (unitary cost 870,- ezk) ,- ezk Total ,- CZK " Measures eligible for co-financi!g - Wild birds, Methods of laboratory Number tests performed per Cost (VAT excluded) analysis method per (unitary cost 900,- ezk) ,- ezk' Virus isolation test ,- ezk (unitary cost 870,- ezk) Total ,- CZK TTAL Wild birds and Poultry ,- CZK 8

105 TSEs 1. The price of the test (unitary cost) including: Test price ther direct material cost (sampling kit, articles of consumption) Wage cost ndirect costs (e.g. protective means and disinfection agent, waste disposal, amortization! depreciation) Handling looses General overhead. 2. All sheep and goats older than 18 months slaughtered, emergency slaughtered C!lld fallen or killed stock to be tested in 2007 in accordance with Commission Regqlcition. No The number of animals to be tested in 2007 has been revised and table was changed. The table 5.2. Summary of the cost has been changed as well. The full new version of the programme you can find in attachment Monitoring of Chronic wasting disease Estimated number of tests Animals to be tested - Wild Red Deer population 299 Animals to be tested - Farmed Red deer 288 Total number of animals to be tested is

106 Estimation of wildlife population Year: 2000 Method of estimation: Hunting bag Species: Fox Region huntil! area (km2) hunted Southern Bohemian r _ _.._---_... Southern Moravian r _----_..._----_.....?L:!?'2':y.?::!.....?......YY.?J?-...?:.1.?L?:.??......?9:. <?......}J? L ?!::...?:?. '..?... Moravia-Silesian r _ _._-_._---...? 9.1.<?.<? :.1.? }:.?......?X. }:?... }: ?!....?:.!. 9:! J..?'!.?.....?!).!!y.!??..1.?... Central Bohemian r _ _..._ ?XQi. :.?.. }?......?)?..... Region ofzlin Czech Republic

107 Estimation of wildlife population Year: 2001 Method of estimation: Hunting bag Species: Fox Region huntin2 area (km2) hunted Southern Bohemian r Southern Moravian r _.._-----_._ ?X!?y ?:?:?......Yy.??:.1.??:?......?X.A?... }J?..}:?-?......?X!:!.?:?.. }:.l... Moravia-Silesian r _ ?X Ql.. :}.? :!!......?X!.. }:?q }:?.....!.?X!?:.1.9. J! ?!!J.!Y..!?-.?... Central Bohemian r _......_----_._ !?.?XQi.:.:...}??:?L... Region of ZHn Czech Republic

108 Estimation of wildlife population Year: 2002 Method of estimation: Hunting bag Species: Fox Region hunted Southern Bohemian r w Southern Moravian r w ?X!.\!?_Y.. _.. _.?:. '.??:......Yy.?...?:.1.? _....?.g?_. _......?X..<?....:??_. L?......?!:g _..?}- -- '.? _.-... Moravia-Silesian r _-----_..._ ? Q.<?. :.1_?_ L??....?L_ }:.?g L?? ?L!._?:.1.9..'.? ?!!.!Y... _??.??... Central Bohemian r _------_ ?xq!i.:.. }?L _....? _._._.. Region ofzlîn Czech Republic

109 Estimation of wildlife population Year: 2003 Method of estimation: Hunting bag Species: Fox Region Southern Bohemian f _---_._._-_.- Southern Moravian f _....??X!?y.?::!:! : ? yy.? _...?}?L ::!.._.....??X..<?......:7.??:?.....?_?!:i!.. _ }} _?::!? hunted Moravia-Silesian f _.._ i??.<?.l.<?.<?... }?. _ }.? _..... i? Ri... :? _..?:??... i??r!.t:..? _?:9...._..i!J.jy.R. J_!..._ Central Bohemian r _._ _ i??xqi.:. _ }? }:?!..... Region of ZHn Czech Republic

110 Estimation of wildlife population Year:2004 Method of estimation: Hunting bag 5Decies: Fox Region buntin!! area (km2) hunted Southern Bohemian r Southern Moravian r ?X!?y :1:!?:? yy.?....?:.t.?l..1:??l......?x..?... }:?..., }? ?!:-!!:. :1..:.?... Moravia-Silesian r _._._._.-...?X 9.t...1:.1.. :??.....??.?L. }:.q :......?X!..?:.1.9:!.?:?-.....?!).!Y...??-... Central Bohemian r _..._..._--_ ?LQ!i.:.; }?L...1:.... Region ofzlin Czecb Republic

111 Estimation of wildlife population Year: 2005 Method of estimation: Hunting bag Species: Fox Region huntin area (km2) hunted Southern Bohemian r _._._- Southern Moravian r _._-...!?.?.?.y?: :?......!?.Yy'?i.?}?.! :?......!?.?X!:.. l.,? } J?.:......!?.?!-:!.?} }?... Moravia-Silesianr _..._._._ !?l.? :.l.?....:? !?.?.!?... }:?......: !?.?!..?}.?:?.....e!!.!y ?? Central Bohemian, r _..._----_ !?.?.i.'.!-:. Region of Zlin :? :.? Czech Republic