Superior Colloidal Stabilization of Beer by Combined Treatment with Silica [Uerogel) and PVPP, Polyclar plus

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1 Voume 37, Number Pages Superior Cooida Stabiization of Beer by Combined Treatment with Siica [Ueroge) and PVPP, Poycar pus Internationa Speciaty Products, Wayne, NJ, USA. This poster was originay presented at the MBAA 112th Anniversary Convention, Keystone, Coorado, SNTiSS Cooida stabiization of beer can be addressed in terms of removing either or both the participating components in the haze formation, i.e., haze-active proine-rich proteins and tannoids and the poypheno precursors in the formation of tannoids. It is a we-estabished practice to use siica ge with high adsorptive capacity for the remova of proteins and cross-inked poyvinypyrroidone (PVPP), for the remova of tannoids, favanos and monomeric and poymeric poyphenos. It is aso a common practice in breweries to use either siica ge or PVPP for cooida stabiization depending upon the beer matrix. It woud be advantageous to investigate the merits and prac- Se puede habar de a estabiizacion cooida de a cerveza en terminos de a eimination de uno o mas componentes participantes en a formation de nieba, por ejempo, proteinas ricas en proina y activas en a nieba y tanoides y 10s precursores poifenoes en a formacion de tanoides. Es una practica bien estabecida e usar &ice geatinosa con ata capacidad de absorcion para a eimination de proteinas y a poivinipirroidona entrecruzada (PVPP), para a eimination de tanoides, favanoes y poifenoes monomericos y poimericos. Tambien es una practica comun en as cervecerias e usar ya sea sfice geatinosa o PVPP para a estabiizacion cooida and PVPP, can be used in combination, either sequentiay or concurrenty, to produce beer with superior cooida stabiization. This composite of Xeroge and PVPP, Poycaf, produced synergy in the remova of both proteins and poyphenos and produced beer with an enhanced shef ife. This resut was evident in the P ant scae trias wi th a Germ an Pisner and an American Lager from a microbrewery. Combination treatment aso showed considerabe improvement in transferabiity of the adsorbents, and beer fitration was observed to be more efficient. Experimenta resuts of these tria.s wi be provided. A working mode to expain the advantages of the combined treatment and the source of synergy wi 1 aso be discussed. Keywords: Cooida Stabiization, Combination Chiproofers, Pus 730, PVPP, Xeroge, Chi Haze Composition a estabiizacion de a cerveza. Se encontro que aunque parecian incompatibes, e xeroge de ata absorcion y e PVPP, pueden ser usados en combination, ya sea secuenciamente o concurrentemente para producir cerveza con una estabiizacion cooida superior. Este compuesto de xeroge y PVPP, produjo sinergia en a eimination de tanto proteinas, coma poifenoes y produjo una cerveza con una vida de estante mejorada. Este resutado fue evidente en prueba.s que se evaron a cabo en pantas con una Pisner Aemana y una Lager Americana provenientes de una micro cerveceria. E tratamiento de combination tambien mostro una mejoria considerabe en a capacidad de transferencia de 10s absorbentes y se observe que a fitration de a cerveza fue mas eficiente. Se proveer& 10s resutados experimentaes de estas pruebas. Tambien se discutirh un modeo de trabajo para expicar as ventajas de1 tratamiento combinado y a fuente de energia. NTRDUCTN Mustafa Rehmanji has over 20 years experience in the mating and brewing industries. He is a Senior Research Chemist with Internationa Speciaty Products. His current interests are in the areas of beer stabiization and technica service for commercia treatment of beverages. Mustafa started his brewing career with Kenya Breweries. Later he moved to Canada and was Director of Technica Service with Prairie Mat Ltd. Mustafa has a BSc. in Chemistry, a Business degree, and a Dipoma in Brewing Technoogy. He is an active member of ASBC and MBAA. Emai: Cooida stabiization of beer can be addressed in terms of removing either or both the participating components in the haze formation i.e. haze-active proine-rich proteins and tannoids; as we as the poypheno precursors in the formation of tannoids. It is found that the seemingy incompatibe xeroge and PVPP (PoycaF) can be used as a composite to produce beer with superior cooida stabiization. The composite of seected xeroge and micronized PVPP gave synergy in the adsorption of both proteins and poyphenos and

2 produced beer with an enhanced shef ife. In addition, it showed improved transferabiity of the adsorbent to the beer and exhibited an efficiency in the fiter runs in the brewery. Drivers for Improving Cooida Stabiity Quaity parameters and shef ife Export beers - ong distribution chains Growth of franchised beers Background - increased market share and production cost pressures Beer Haze Definitions Bioogica Haze - microbia origin Non-Bioogica Haze - presence of cooida matter makes beer appear hazy Chi Haze - formed when beer is cooed to 0 C; re-dissoves on warming to 20 C. (Increases during storage) Permanent Haze - irreversibe haze, remains in beer at 20 C *O Rourke et a Chii haze FIGURE 1 Mode of Haze Formation* Permanent haze TABLE 1 Materia & Processes to Optimize Cooida Stabiity Poyphenos Condensed Poypheno Tannoids Process Poypheno Protein Reduction Process Optimisation Reduction \ y Raw materia Low Low protein (N) Low mat modification seection proanthocyanidin barey Coarse grind of mat mat Hop extract Brewhouse High adjunct Mashing process, Vigorous kette boi for >60 ratio ph & temperature min. Avoid weak Good hot break Avoid excess minera sats worts Cod wort fitration Fermentation Rapid onset of Eary yeast remova Minimum 7 days and fermentation maturation at Maturation -1 C Fitration Low soids count Fiter at -1 C Avoid O2 pickup.. CoLechin Gaocatechin Prodephinidin FIGURE 2 Haze-Active Poyphenos I I Adsorption: - Bentonite - Siicage T 1 Composition of Chi Haze Simutaneous Adsorption -Poycar Pus 730 I Adsorption: I POLYPHENOLS About 17% Protein-Tannoid Remova: -Chiing -Formadehyde Compex FIGURE 3 Cooida Stabiization of Beer

3 KEY FEATURES Remove haze-forming Poyphenos Remove haze-forming Proteins Remove both haze-forming poyphenos & proteins - combined stabiization Combined stabiization is the best option for good physica stabiity Poycar Pus Optimized ratio for preventing chi and permanent haze 0 High adsorptive capacity for haze forming precursors 0 Improved handing and performance characteristics PROPERTIES OF POLYCLAR PLUS 730 Simutaneous remova of: 0 Proteins 0 Tannoids 0 Poypheno precursors of tannoids Handing features: 0 PVPP enabes foccuation of hydrated xeroge partices 0 Improves materia dispersion and transfer 0 Avoids setting at the bottom of surry tanks *Preferred moecuar conformation with catechin Xeroge PVPP FIGURE 4 PVPP-Poypheno Interaction* par - free to move around each other f suspensions - settes and compacts - PACKS - ess abe to pack cosey - minimum void voume - greater voids & swe voume Premix No temperature change when mixed FIGURE 6 Non-adsorbing Foccuation of Stabiizer Partices Greater than PVPP (g/hi) Dose eve of stabiizers required to achieve 12 months of shef ife (Lager 12 EBC) Courtesy: Ian McMurrough FIGURE 5 Cooida Stabiization with Different Maturation Times WHAT IS POLYCLAR PLUS 730? A proprietary composite of micronized Poyvinypoypyrroidone (PVPP) and seected Siica Xeroge PVPP Xeroge Poycar Pus 730 Inversions required to re-disperse 10% surries after 24 hours of sedimentation. Improved dispersion resuting in a more homogeneous dosing into the beer. FIGURE 7 Re-dispersion of Poycar Pus 730

4 TABLE 2 Partice size distribution in the DRY state Sampe Percenties (urn) Mv 10% 50% 90% Poycar Xeroge Poycar Pus Comparison with components Partice size distribution of Poycar Pus 730, denoted by Mv (mean voume), fas between the partice size distribution of the component constituents, Poycar and Xeroge. FIGURE 8 (eft) Partice Size Distribution of Poycar Pus Dry TABLE 3 Partice size distribution in the HYDRATED state Sampe Percenties (urn) Mv 10% 50% 90% Poycar I Xeroge I Poycar Pus Comparison with components The partice size of hydrated Poycar Pus 730 is arger than both its constituents - see tabe. These arger partices do not compact easiy. This non-compaction gave improved fiter runs in the brewery. At the same time, the adsorption of poypheno or protein was not affected. In fact, in some instances the adsorption was enhanced. FIGURE 9 (eft) Partice Size Distribution Poycar Pus % Hydrated

5 40 60 PVPP in Bend (%) *Schenk method FFI max 25% - 45% PVPP 0 I I I Contact Time (min.) Unstabiized Lager FIGURE 10 Fiter Fow Rate Index* FIGURE 13 Sensitive Protein Reduction with Poycar Pus F 80 - $ 60 2 c/ 40-0 z Time Xeroge settes and compacts. Poycar Pus 730 dose not prematurey sediment. Therefore, Poycar Pus 730 is dosed in to beer eveny and provides enhanced adsorption of Proteins and Poyphenos. (hrs) // Unstabiized It+20 g/hi g/hi +60 g/hi Contact Time (min.) Lager FIGURE 11 FIGURE 14 Sedimentation of PVPP, Xeroge and Poycar Pus 730 Poypheno Haze Stabiization with Poycar Pus *20 g/hi +30 g/hi +60 g/hi ~,.....,,.... I I I.,. +30 g/hi 60 g/hi 0 5 IO IO Contact Time (min.) Contact Time (min.) Unstabiized Lager Unstabiized Lager FIGURE 12 FIGURE 15 Protein Haze Stabiization with Poycar Pus 730 Tannoid Reduction with Poycar Pus 730

6 10 80 I.j : -? 70 * Data tempate courtesy of Schneider, 1 anayzed by PT Standard 20 VERY HIGH 10 POLYPHENOL INSTABILITY : STABILITY \ 1 I 1 I I I I 1 I Poypheno Stabiity (m reagent/ OOm beer to 2 EBC) Si hydroge 95g/h Si hydroge 95g/h + PVPP 20g/h Poycar Pus g/h Poycar Pus g/h Poycar Pus g/h Summary FIGURE 16 of Beer Stabiization FIGURE 18 Forced Haze Deveopment in Packaged Beer - Tria A OPTIONS FOR ADDITION OF POLYCLAR PLUS 730 US MICROBREWERY TRIAL B Between fermentation and maturation vesses Between maturation and fiter inet buffer tank Via diatomaceous earth dosing pot Via separate dosing pot prior to primary fiter After primary fitration, before a secondary fiter Objective: to evauate stabiization with Poycar Pus bb of American Lager treated Stabiizer dosed in-ine, before buffer tank Dosage rate of Poycar Pus Ob/OObb DE PVPP Stabiized - 10 b/ OObb 40 - E Predicted Shef Life in Months 30 / 20 - E 10 -bunstabiized Region Untreated Poycar Pus 730 Increasing Poypheno Stabiity ==> (m T125/1 OOm Beer to 2 EBC Additiona To Packaging Poish Fiter FIGURE 19 Tria B: Stabiization FIGURE 17 Possibe Injection Points for Poycar Pus 730 Haze) I week at 37 C with Tota Haze c 2.0 EBC is equivaent to I month of Shef Life Resuts EUROPEAN BREWERY TRIAL A Objective: to increase product shef ife Current stabiization: 95g/h siica hydroge 500h fiter run ength Stabiizer dosed in ine before primary fiter Contact time - 10 minutes Test 1: Siica hydroge + PVPP A NEW DIRECTION WITH POLYCLAR PLUS 730 Combined stabiization Singe addition Utiizes existing equipment Lower addition rates Appicabe to different beer types Exceent handing & performance Efficient cooida stabiization Test 2: Poycar Pus 730 We thank ISP for permission to present this poster

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