Supplemental Figure 1. Seedlings of Transgenic and Mutant Plants.
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1 Supplemental Figure 1. Seedlings of Transgenic and Mutant Plants. Supplemental Figure 2. Role of SPLs in Anthocyanin Accumulation. (A) Inflorescences of spl mutants. Scale bar represents 0.5 cm. (B) Close-ups of rosette-stem junctions of ProSPL9:rSPL9, ProSPL10:rSPL10, and Pro35S:rSPL3 plants.
2 Supplemental Figure 3. Quantification of Putative Phenolic Derivatives. Peak areas of putative phenolic derivatives [peaks 3 to 7 in (Figure 2A to 2C)]. Errors bars indicate ±SD (n=3). Supplemental Figure 4. Inflorescences of Pro35S:MIR156 in tt8-1, pap1- migs, and ttg1-1 backgrounds.
3 Supplemental Figure 5. SPL9 Binding to MYB113 in Yeast. The coding region of MYB113 and SPL9 was fused to AD or BD, respectively. Only the yeast harboring both MYB113-AD and SPL9-BD could grow on LTH plates supplemented with 15 mm 3-AT.
4 Supplemental Table 1. Oligonucleotide Primer Sequences. ID Gene Sequence (5-3 ) Purpose G DFR TGC CTG CAG GCG ATA CAA AAT TCT TCG ATT TTT AAA promoter G DFR CGG GGA TCC TTT TGT GGT TAT ATG ATA GAT TGT GCT promoter G DFR GTA AAA GTG GGT GGG GAA CAA AA ChIP, region I G DFR GAA AAC AGA TAA TAA GAA CGA GAA GC ChIP, region I G DFR CAA CAA AAT ACA CAC CTA AGG AAA T ChIP, region II G DFR TAT ATG ATA GAT TGT GCT TTG GGA A ChIP, region II G DFR CGA AAT CAA GCG AAG ATC TGA TC ChIP, region III G DFR CAA TTT GTT GTA TCG TAA GAC GTC AG ChIP, region III G DFR AGC CGC CAA GGG ACG TTA TAT TTG qpcr G DFR CCG GGA GAA AAC CCT TTT GAC GA qpcr G FLS1 CCA CCG TCA TGC GTC AAT TAC AG qpcr G FLS1 TCT CCG CCG AGA CCT TCT TTC AA qpcr G CHI CTC TCT TAC GGT TGC GTT TTC G qpcr G CHI CAC CGT TCT TCC CGA TGA TAG A qpcr G CHS GGA GAA GTT CAA GCG CAT GTG qpcr G CHS ATG TGA CGT TTC CGA ATT GTC G qpcr G PAP1 TGC TGG AAG ATT ACC TGG TCG qpcr G PAP1 AGT GCC GGT GTT GTA GGA ATG qpcr G TT8 TGG AGA CAC CAT TGC GTA CGT qpcr G TT8 TCT TAC AAG TAC GCG TCC GCT qpcr G MYB11 GGC GAT TGT AAC CCA AGC ATT qpcr G MYB11 TCA CAT GAG GAC ACG TGG ACA qpcr G TT2 CCG CAA AAG ACT TCC CAA AA qpcr G TT2 GCA CCT AAT CGC CTT TGT ACG T qpcr
5 G TTG1 TCG ATA TTC GTT CGC CGA CT qpcr G TTG1 GCC TGT GTA TCA TCA CCA CCA G qpcr G PAL1 CTT GGA ACA GAG CTT TTG ACC G qpcr G PAL1 CGT GAA AAC CTT GTC GAA CTC TTC qpcr G FLS3 GCT TGT GGC AAG TGC GGT GG qpcr G FLS3 GCT CGG TCG GGA TTC CGT GG qpcr N-0078 TUB GAG CCT TAC AAC GCT ACT CTG TCT GTC qpcr N-0079 TUB ACA CCA GAC ATA GTA GCA GAA ATC AAG qpcr G MYB12 AAC AGG TGG TCA CTA ATC GCG qpcr G MYB12 TTC GTT GTC TGT TCT CCC TGG qpcr G MYB111 CGG CCT CAC AAT GTT TCT CAC qpcr G MYB111 CCA CTC ATA AGG CCC CAA AGA qpcr G EGL3 CAT CAG CTA ATA CTC GGA CCG C qpcr G EGL3 TCT GCG ATT TCT CTC CCA ATG T qpcr G GL3 TCG GTT CGT TTG GTA ATG AGG qpcr G GL3 GCT TGC AAT TGA CGG TTA AGC qpcr G ANS GGC TGT GTT TTG TGA GCC ACC A qpcr G ANS CCT TGG AGG AAA CTT AGC CGG AGA qpcr G UGT75C1 CGG TGT TGG AGA GTG TAT CGG qpcr G UGT75C1 TGA TCC CCA AAA AAT GGC C qpcr G UGT78D2 TTT TGG CGC ATT GTG CTG T qpcr G UGT78D2 TCA GCA AAC TGC GGA AAC G qpcr G MYBL2 ATG AAC AAA ACC CGC CTT CG qpcr G MYBL2 TCA TCG GAA TAG AAG AAG CG qpcr G MYB113 ATC TTG TTC TTC GCC TTC ATA AA qpcr G MYB113 GCA TCG TTC ATC GTG CTT CTT A qpcr
6 G F3 H TTC CTT ACC TTC AGG CGG TTA TC qpcr G F3 H CGA GAG TGG TGT TGG TGG ATG qpcr G SPL3 ACG CTT AGC TGG ACA CAA CGA GAG AAG qpcr G SPL3 TGG AGA AAC AGA CAG AGA CAC AGA GGA qpcr N-1461 SPL4 TCC CAT GGC ATG GAG GGT AAG AGA TCA CAA G qpcr N-1462 SPL4 TCA GAA TTC CTA TCT AAT CTG TGG TCG CTT G qpcr G SPL9 CAA GGT TCA GTT GGT GGA GGA qpcr G SPL9 TGA AGA AGC TCG CCA TGT ATT G qpcr G SPL15 TGA ATG TTT TAT CAC ATG GAA GCT C qpcr G SPL15 TCA TCG AGT CGA AAC CAG AAG AT qpcr G DFR AAA GGA TCC TTT TGT GGT TAT ATG ATA GAT TGT GCT TTG GGA AAG ATT CAA CTA TGT ATG TTC GGT ATA AAT G DFR ATT GTT ACA TAC CAC ACA TCT CTT TAG TCC TTC GTC AAC CAA CGT TCC CCA CGT GCT TCT CCG GTT GAT ACT G DFR AGA TGT GTG GTA TGT AAC AAT CTG GTA AGT TGA TTT TAT TAT TTC CTT AGG TGT GTA TTT TGT TGT TGT ATC GGT Promoter mutation Promoter mutation Promoter mutation G-7981 SPL9 AAA CCC GGG ATG GAG ATG GGT TCC AAC TCG GGT G-7982 SPL9 AAA GAG CTC TCA GAG AGA CCA GTT GGT ATG GTG A G PAP1 ACG GGA TCC GAA TGG AGG GTT CGT CCA AAG GGC TGC GA, MIGS G PAP1 CTC GAG CTC CTA ATC AAA TTT CAC AGT CTC TCC ATC, MIGS G R2R3 CTC GAG CTC CTA ACA CGG TTC ATG TTT CTT ACT CAG ATG G TT8 GCC GAA TTC ATG GAT GAA TCA AGT ATT ATT CCG GCA GAG G TT8 GAC GGA TCC CTA TAG ATT AGT ATC ATG TAT TAT GAC TTG G GL1 GCC GAA TTC ATG AGA ATA AGG AGA AGA GAT GAA AAA GAG
7 G GL1 GAC GGA TCC CTA AAG GCA GTA CTC AAC ATC ACC AGA AGC G TT2 GCT CAT ATG GGA AAG AGA GCA ACT ACT AGT GTG AGG G TT2 CTC GAG CTC TCA ACA AGT GAA GTC TCG GAG CCA ATC TTC G MYB113 GCT CAT ATG GGC GAA TCA CCC AAA GGG TTG AGA AAA G MYB113 CTC GAG CTC CTA ATT CAG TTC TAA AGT CTC TTC ATC AAA G SPL9 GGC GGT ACC ATG GAG ATG GGT TCC AAC TCG GGT CCG G SPL9 CTG GTC GAC GAG AGA CCA GTT GGT ATG GTG AGA AGA AGA G TT8 GGC GGT ACC ATG GAT GAA TCA AGT ATT ATT CCG GCA GAG G TT8 CAG GTC GAC CTA TAG ATT AGT ATC ATG TAT TAT GAC TTG G PAP1 CTC GGT ACC ATG GAG GGT TCG TCC AAA GGG CTG G PAP1 CTG GTC GAC ATC AAA TTT CAC AGT CTC TCC ATC GAA G PAP1 CTG GTC GAC TTA ATC AAA TTT CAC AGT CTC TCC ATC GAA AAG G PAP1 ATC AAA TTT CAC AGT CTC TCC ATC GAA AAG PAP1-HA G SPL9 TCA CTT ATC ATC ATC ATC CTT GTA ATC GAG AGA CCA GTT GGT ATG GTG AGA rspl9-flag
8 Supplemental Table 2. Constructs. ID Gene Restriction Sites Backbone Purpose JW626 SPL9 EcoRI pgbkt7 JW770 PAP1 BamHI/SacI pgadt7 JW839 TT8 EcoRI/BamHI pgbkt7 JW853 GL1 EcoRI/BamHI pgbkt7 JW894 PAP1, R2R3 BamHI/SacI pgadt7 JW895 TT2 NdeI/SacI pgadt7 JW896 MYB113 NdeI/SacI pgadt7 JW771 nluc HindIII/EcoRI pcambia2300 JW772 cluc HindIII/EcoRI pcambia2300 JW840 rspl9-nluc KpnI/SalI JW771 JW841 cluc-pap1 KpnI/SalI JW772 JW813 PAP1-nLUC KpnI/SalI JW771 JW838 cluc-tt8 KpnI/SalI JW772 JW491 pdfr PstI/BamHI pcambia2300 Promoter GUS JW843 pdfr mu PstI/BamHI pcambia3300 Promoter GUS JW647 PAP1-migs BamHI 173_CH42 MIGS JW689 rspl9-flag XbaI/SacI pcambia2300 CoIP JW877 PAP1-HA XbaI/SacI pcambia2300 CoIP
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