Immunological Identification

Transcription

1 Immunological Identification Tests Bio-Rad kits are used for the identification of bacteria isolated on culture media or for direct detection in specimens. These tests are based on a latex particle agglutination reactionthat is read visually by the presence of colored particles. PASTOREX STAPH PLUS Visibly More Sensitive Pastorex Staph Plus is a rapid agglutination test for the simultaneous detection of the fibrinogen affinity antigen (clumping factor), protein A, and the capsular polysaccharides of Staphylococcus aureus. The kit includes the control, disposable cards and sticks. 21 identification MICROBIOLOGY A growing percentage of S. aureus (mainly METHI-R strains) can elude detection in some agglutination tests because cell wall antigens (clumping factor and protein A) are masked. The Pastorex Staph Plus kit incorporates monoclonal antibodies to capsular polysaccharides as well as to cell wall antigens, thus reducing the risk of false negative results. Unmask METHI-R: Pastorex Staph Plus allows the simultaneous detection of cell wall antigens and capsular antigens of S. aureus. Pastorex Staph Plus disposable card Pastorex Staph Plus...50 tests Pastorex Staph Plus...5 x 50 tests

2 22 MICROBIOLOGY identification PASTOREX STREP Visibly Easier A family of rapid, sensitive agglutination tests providing identification of β-hemolytic streptococci belonging to Lancefield groups A, B, C, D, F and G. Identification is based on the type of hemolysis (β-hemolysis) surrounding colonies grown on blood agar and on the detection of groupspecific polyoside antigens in the cell wall. The polyoside antigens are extracted from the cell wall and their presence demonstrated using latex particles coated with homologous antibodies. Vial caps are color-coded Sensitivity is increased using a reduced inoculum due to optimized enzyme and extraction volumes Extraction can be performed at two temperatures: 15 minutes at room temperature 10 minutes at 37 C Bichromatic results enhances readability: Positive: Red agglutination reaction on green background Negative: Homogenous brown suspension Pastorex Strep disposable agglutination card Pastorex Strep: A, B, C, D, F, G (complete kit including extraction enzyme, controls, disposable cards and sticks)...60 tests Pastorex Strep A...60 tests Pastorex Strep B...60 tests Pastorex Strep D...60 tests Extraction Enzyme (for Pastorex Strep) (individual kits)...2 x 10 ml Disposable Agglutination Cards...3 x 15 cards

3 23 identification MICROBIOLOGY PASTOREX MENINGITIS Visibly More Effective For the Detection of N. meningitidis A, B/E. coli K1, C, Y/W135, H. Influenzae Type b, S. pneumoniae, Streptococcus B. Bacterial meningitis is an infection of the meninges and can be either an acute or chronic disease depending on the infective agent. The primary agents causing bacterial meningitis, an infection of the brain meninges, are Neisseria meningitidis, Streptococcus pneumoniae, Haemophilus influenzae Type b, and group-b streptococci. Because meningitis is a life-threatening disease, rapid identification is critical for the initiation of correct antimicrobial therapy. The Pastorex Meningitis kit consists of colored latex particles coated with mouse monoclonal or rabbit polyclonal antibodies for the direct qualitative detection and identification of Neisseria meningitidis, A, B/E coli K1, C, Y/W 135; Haemophilus influenzae Type b; Streptococcus pneumoniae; and group B streptococci. Pastorex Meningitis complete kit includes controls, disposable cards and sticks. Kit Features: Rapid diagnoses from CSF, serum, and urine specimens Confirmation of culture isolates Clear and reliable reading with seven colored latex suspensions Control for non-specific reactions using the negative Control Optimized reaction time with increased sensitivity Pastorex Meningitis: N. meningitidis A, B/E. coli K1, C, Y/W135, H.Influenzae Type b, S. pneumoniae, group B streptococci (complete kit includes controls, disposable cards and sticks)...25 tests Pastorex Meningitis: Meningo A...25 tests Pastorex Meningitis: Meningo B / E. coli K tests Pastorex Meningitis: Meningo C...25 tests Pastorex Meningitis: Streptococcus B...25 tests Pastorex Meningitis: Streptococcus pneumoniae...25 tests Pastorex Meningitis: Haemophilus Influenzae Type b...25 tests Pastorex Meningitis: Diluent...40 ml Pastorex Meningitis: Control Reagents...2 x 25 tests PASTOREX ROTAVIRUS Pastorex Rotavirus (see Direct Ag Detection)...30 tests

4 40 MICROBIOLOGY mycology Serology Antigen and Antibody Tests Invasive fungal infections have become more frequent over recent years. This increased incidence is due to the rising number of immunocompromised patients: treatment of malignant diseases (chemotherapy) and organ transplantations, particularly bone marrow transplantations (immunosuppressive agents, corticosteroids). AIDS is also associated with an increased risk of fungal infections, which represent a major cause of morbidity and mortality. The main fungal infections occurring in this context are candidiasis, aspergillosis and cryptococcosis. The development of new serological tests has improved the diagnosis of these diseases. These tests are designed to provide rapid results, since patient survival depends on early diagnosis and rapid initiation of appropriate antifungal therapy. Bio-Rad offers a complete line of tests for the detection of soluble antigens of Candida, Aspergillus and Cryptococcus neoformans and for the detection of antibodies in patient sera using standardized antigens of Candida and Aspergillus. Antigen Detection Aspergillus Aspergillus species are known to release exo-antigens during growth: low levels of the circulating galactomannan antigen are present in biological fluids of patients with invasive aspergillosis. The sandwich ELISA Platelia Aspergillus can detect 1ng of galactomanann antigen per ml of serum, allowing the diagnosis of invasive aspergillosis at early stage of the disease often before the onset of clinical signs and radiological abnormalities. This method is now integrated into the EORTC-MSG criteria of definition and classification of invasive aspergillosis. Candida Candida infections are the most frequent form of nosocomial fungal infections. The detection of circulating antigens in the serum improves their diagnosis: Platelia Candida Ag detects the mannan antigen that appears to be one of the main markers of invasive candidiasis. The combination of this test with Platelia Candida Ab/Ac/Ak (detecting anti-mannan antibodies) improves the sensitivity and allows an earlier diagnosis of invasive candidiasis. Antigen and antibody detection product ordering information continues on the next page >

5 41 mycology MICROBIOLOGY Cryptococcus Cryptococcosis is an opportunistic infection that occurs in patients who are immunodeficient, most notably those with AIDS. In these patients, the infection disseminates rapidly to the central nervous system (meninges) and other organs after a pulmonary primary phase. Soluble antigens become rapidly detectable in biological fluids. Pastorex Crypto Plus is a latex agglutination test designed for the qualitative and semi-quantitative detection of the capsular polysaccharide of Cryptococcus neoformans (glycuronoxylomannan) in serum, CSF, BAL and urine. EIA Microplate Platelia Aspergillus (for the detection of Aspergillus galactomannan antigen in serum by enzyme immunoassay) tests Platelia Candida Ag (for the detection of Candida mannan antigen in serum by enzyme immunoassay) tests Pastorex Aspergillus (for the detection of Aspergillus galactomannan antigen in serum by latex agglutination technique) tests Pastorex Candida (for the detection of Candida mannan antigen in serum by latex agglutination technique) tests Pastorex Crypto Plus (for the detection of Cryptococcus neoformans glycuronoxylomannan antigen in serum, CSF, BAL, urine, by latex agglutination technique)...60 tests Platelia Aspergillus EIA