About Our Products. Blood Products. Purified Infectious/Inactivated Agents. Native & Recombinant Viral Proteins. DNA Controls and Primers for PCR

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2 About Our Products Purified Infectious/Inactivated Agents ABI produces a variety of specialized reagents, allowing researchers to choose the best preparations for their studies. Available reagents include purified virus, purified viral lysate, whole inactivated virus, and high-titer infectious virus preparations. ABI offers custom start-to-finish virus propagation, purification, characterization, and inactivation services. We also specialize in large scale, in vitro production of HIV and other viruses. DNA Controls and Primers for PCR ABI s DNA controls can be used as positive controls in nucleic acid amplification reactions. Quantitated controls can also be used to generate standard curves for quantitative PCR assays, and in real-time quantitated PCR to determine the copy number of unknown samples. The preparations from whole virus, virus infected cells, or cultured bacteria, allow researchers to amplify or test any region of the genome which is present in natural infections. Additionally, PCR primers are available to complement select DNA controls. Infectious Disease Antigens ABI s Infectious Disease Antigens are carefully optimized and characterized to assure high sensitivity and specificity in a variety of antibody detection assay formats. In addition to virus infected cell extracts, ABI offers various uninfected cell extracts for use as negative controls. Antigen purification strategies vary according to each product. Blood Products Human Blood derived products are offered for use in infectious disease research. Cryopreserved elutriated lymphocytes and monocytes/macrophages are prepared from single donor blood and may be purchased as frozen cell ampules. Other blood products such as Heat-Inactivated Human AB+ Serum and Purified Human Interleukin-2 are available as supplements for primary cell culture. Native & Recombinant Viral Proteins ABI s Purified Native Viral Proteins are whole viral antigens that have been immunoaffinity purified from clarified infected cell extracts. ABI offers purified native viral proteins from virus-infected cell culture, isolated from conditioned cell culture medium, inactivated by Triton X-100, and then purified by immunoaffinity chromatography or reverse phase HPLC to >95% purity. Our EBNA-1 Purified Recombinant Protein is a full-length EBNA-1 gene cloned and produced in a Baculovirus protein expression system. EBNA-1 is then purified by ion-exchange chromatography to >95% purity. Quantitated Viral Load Controls Our new Quantitated Viral Load Controls can be used in nucleic acid extraction and amplification processes alongside unknown samples to measure viral load and as a control to validate, monitor, and troubleshoot testing procedures. Quantitated Viral Load Controls contain a known concentration of purified, whole virus Monoclonal Antibodies ABI offers monoclonal and polyclonal antibodies against infectious agents. These antibodies are directed against specific human viral proteins and are highly specific in immunoassays. ABI hybridomas are produced by standard fusion and selection methodologies to yield the highest quality monoclonal antibodies possible for a specific application. ELISA Test Kits ABI s ELISA (Enzyme-Linked Immunosorbent Assay)research test kits are intended for qualitative and semi-quantitative detection of IgG antibody in human serum or plasma. All test kits are evaluated against a standard panel of samples to ensure that product specificity and sensitivity claims are met. Kits are reliable and easy to use, and produce rapid results. All necessary reagents are included to perform multiple individual assays. particles suspended in a buffer appropriate for further dilution in the same matrix as the unknown sample.

3 Custom Designed Products & Services Because your research is unique, ABI offers a variety of custom services to suit your specific requirements. We place great emphasis on product quality. Our products are manufactured to the highest standards and face stringent, comprehensive quality control testing during multiple stages of production, and upon completion. Cell & Infectious Agent Propagation ABI can use your provided cell line to grow high-quality cell seed stocks and create Master or Working Cell Banks (MCB or WCB). ABI can also use your provided virus stock to propagate your custom material. Because Quality Control (QC) testing is a critical part of ABI s production process, sterility testing, Mycoplasma testing, and a viable cell count are performed on every custom cell culture product. Concentration/Purification Some research projects demand a high-purity concentrated virus, while others demand a high titer. And while all of ABI s concentrated viral and bacterial preparations undergo rigorous quality control testing, the individual assays required to determine product specifications may vary according to your specific research needs. ABI provides multiple options to help researchers customize their viral and bacterial preparations. Our Purified Virus products assure the highest purity viral preparations. Inactivation Inactivated viral and bacterial preparations are essential for some investigations. ABI offers custom inactivation services for any infectious agent in our inventory. Additionally, inactivations can be performed on samples submitted to ABI, or on custom viral or bacterial preparations. Electron Microscopy For over 30 years, our Electron Microscopy (EM) services have allowed investigators to examine fine structure for the presence, absence, and quantitation of known microbial or adventitious agents in specimens. Utilizing negative staining technology, our scientists are able to conduct extensive analysis for virus characterization. We provide the investigator with the recommended reagents and protocols needed to properly prepare their specimens for EM examination. All micrographs produced at ABI are developed and printed to publication quality standards, and ABI stores all grids, blocks, negatives, and records for the investigator. Quick. Easy. Technical Support. Send Us Your Questions HHV-6 HHV-6

4 Standard Products Blood Products Purified Human Interleukin Adult Human Elutriated Monocytes - Frozen Adult Human Elutriated Lymphocytes - Frozen P Human AB+ Serum, Heat-Inactivated Standard PCR Controls HIV-1 (IIIB Strain) Infected Cell DNA HIV-2 (NIH-Z Strain) Infected Cell DNA HTLV-II (C3-44/Mo Strain) Infected Cell DNA HTLV-I (MT-2 Strain) Infected Cell DNA Human Adenovirus-2 Infected Cell DNA HPV-16 Transformed Cell DNA HPV-18 Transformed Cell DNA Hepatitis C Viral RNA Quantitated PCR Controls HSV-1 (MacIntyre Strain) Quantitated Viral DNA HSV-2 (G Strain) Quantitated Viral DNA HHV-6B (Z-29 Strain) Quantitated Viral DNA Neisseria gonorrhoeae Quantitated Bacterial DNA HCMV (AD169 Strain) Quantitated Viral DNA EBV (B95-8 Strain) Quantitated Viral DNA VZV (Rod Strain) Quantitated Viral DNA Chlamydia trachomatis LGV-II (434 Strain) Quantitated DNA Chlamydophila pneumoniae (CDC-CWL-011 Strain) Quant DNA KSHV/HHV-8 (KS-1 Strain) Quantitated Viral DNA BK Virus (MM Strain) Quantitated Viral DNA JC Virus (MAD1 Strain) Quantitated Viral DNA HHV-6A (GS Strain) Quantitated Viral DNA Human Adenovirus-5 Quantitated Viral DNA Toxoplasma gondii (RH Strain) Quantitated DNA Mycobacterium tuberculosis (H37Rv Strain) Quantitated Bact DNA HHV-7 (H7-4 Strain) Quantitated Viral DNA Mycoplasma pneumoniae (M2 Strain) Quantitated DNA Primers For PCR P HSV-1 Forward and Reverse Primers P HSV-2 Forward and Reverse Primers P HHV-6B Forward and Reverse Primers P Neisseria gonorrhoeae Forward and Reverse Primers P HCMV Forward and Reverse Primers P EBV Type 1 Forward and Reverse Primers P VZV Forward and Reverse Primers P BK Human Polyomavirus Forward and Reverse Primers P Mycoplasma pneumoniae Forward and Reverse Primers Quantitated Viral Load Controls T HCMV Quantitated Viral Load Control T HHV-6B (Z-29 Strain) Quantitated Viral Load Control T EBV (B-958 Strain) Quantitated Viral Load Control T HSV-1 (MacIntyre Strain) Quantitated Viral Load Control Purified Infectious Agents/Inactivated Infectious Agents HSV-1 (MacIntyre Strain) Purified Virus HTLV-I (MT-2 Strain) Purified Virus HTLV-I (MT-2 Strain) Purified Virus HTLV-I (MT-2 Strain) Purified Viral Lysate EBV (B95-8 Strain) Purified Virus EBV (B95-8 Strain) Purified Virus HIV-1 (IIIB Strain) Purified Virus HIV-1 (IIIB Strain) Purified Virus HIV-1 (IIIB Strain) Purified Viral Lysate (H9 Cells) HIV-1 (IIIB Strain) Purified Viral Lysate (HuT 78 Cells) HIV-1 (IIIB Strain) Direct Pelleted Virus (1000X) HIV-1 (IIIB Strain) Direct Pelleted Virus (1000X) HIV-2 (NIH-Z Strain) Purified Virus HIV-2 (NIH-Z Strain) Purified Viral Lysate HCMV (AD169 Strain) Purified Viral Lysate HSV-1 (MacIntyre Strain) Purified Viral Lysate HSV-2 (G Strain) Purified Viral Lysate EBV (B95-8 Strain) Purified Viral Lysate SIV (mac251 Strain) Purified Virus SIV (mac251 Strain) Purified Virus SIV (mac251 Strain) Purified Viral Lysate SRV-1 (Macaque Strain) Purified Virus SRV-1 (Macaque Strain) Purified Virus SRV-1 (Macaque Strain) Purified Viral Lysate HIV-1 (Ba-L Strain) Direct Pelleted Virus (500X) Human Adenovirus-5 Purified Virus Human Influenza A/Hong Kong/8/68 (H3N2) Purified Virus Human Influenza A/PR/8/34 (H1N1) Purified Virus SRV-2 Purified Virus Human Influenza B/Lee/40 Purified Virus SRV-5 Purified Virus SRV-5 Purified Virus HIV-1 (phxb2d/iiib Strain) Direct Pelleted Virus (500X) HIV-1 (phxb2d/iiib Strain) Direct Pelleted Virus (500X) HHV-6A (GS Strain) Purified Viral Lysate RSV-A (A2 Strain) Purified Virus RSV-A (A2 Strain) Purified Virus RSV-A (A2 Strain) Purified Viral Lysate RSV-A (A2 Strain) Direct Pelleted Virus (1000X) RSV-A (A2 Strain) Direct Pelleted Virus (1000X) FeLV (Thielen Strain) Purified Virus Vaccinia (Lister Strain) Purified Virus Vaccinia (Lister Strain) Purified Virus, UV-Inactivated Vaccinia (WR Strain) Purified Virus Vaccinia (WR Strain) Purified Virus, UV-Inactivated Human Influenza A/2/Japan/305/57 (H2N2) Purified Virus

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