Some Immunological Test. Presented by Alaa Faeiz Ashwaaq Dyaa Aseel Abd AL-Razaq Supervised by D.Feras

Save this PDF as:
 WORD  PNG  TXT  JPG

Size: px
Start display at page:

Download "Some Immunological Test. Presented by Alaa Faeiz Ashwaaq Dyaa Aseel Abd AL-Razaq Supervised by D.Feras"

Transcription

1 Some Immunological Test Presented by Alaa Faeiz Ashwaaq Dyaa Aseel Abd AL-Razaq Supervised by D.Feras

2 Alaa Faeiz

3 Antigen -Antibody Reactions. Antigen antibody reactions are performed to determine the presence of either the antigen or antibody. ( serological tests ). One of the two components has to be known. e.g. with a known antigen, such as influenza virus, a test can determine whether antibody to the virus is present or not.

4 There are different types of antigen antibody ractions. Include: - Precipitation reactions(single and double) - Agglutination reactions(latex, haemagglutination and bacterial Ags ) -Labeled Antibody serology include: -ELISA -Immuno-fluorescence assay - Radio Immuno Assay (RIA)

5 Precipitation test : In this test antigen is in soluble form (solution). Antibody cross -links antigen molecules to form aggregates (precipitates) in the zone of equivalence:at optimal proportion of antigen and antibody. Precipitation test can be performed in solution or in semi- solid medium (agar).

6 Precipitation in Agar. Single radial immunodiffusion:(mancini TEST) Antibody is incorporated into agar and antigen introduced into the well. As antigen diffuses into agar precipitation rings form depending on the concentration of the antigen. This technique is commonly used for determining the concentrations of various plasma proteins such as IgG, and IgM in patients suspected to be suffering from agamma-globulinaemia and multiple myeloma, respectively and others.

7 Single Radial Immunodiffusion.

8 Double immunodiffusion.(ouchterlony analysis) Antigen and antibody are placed in different wells in agar and allowed to diffuse and form precipitation lines at the points of optimal concentrations. This method is used to determine whether antigens are related, identical or non identical.

9 Double immunodiffusion

10 Ashwaaq Dyaa

11 Agglutination In this test the antigen is particulate (e.g. bacteria and red blood cells) or an inert particle (latex beads) coated with antigen. Antibody is divalent and cross links the multivalent antigen to form a lattice network or clumps (agglutination). This reaction can be performed in a tube or on a glass slide e.g. ABO blood grouping.

12 Latex agglutination This test is a laboratory method to check for certain antibodies or antigens in a variety of bodily fluids including saliva, urine, cerebrospinal fluid, or blood. Examples of tests based on latex agglutination reaction include pregnancy test, C-reactive protein,, and IgM rheumatoid factors and others.

13 the sample is mixed with latex beads coated with a specific antibody or antigen. If the suspected substance is present, the latex beads will clump together (agglutinate)

14 Antigen Antibody Reactions Haemaggultination Tests: The clumping or clustering of red blood cells caused by certain viruses, antibodies, or other substances. A.Simple haemagglutination : when the red blood cells agglutinated by anti sera directed to them.

15 B.passive haemagglutination Red cells can also absorb many antigens and when mixed with specific antibodies will form clumps i.e. red cells are passive carriers.

16 Agglutination test of suspensions The most common example of this category is WIDAL TEST Widal test is a tube agglutination test employed in the serological diagnosis of enteric fever. Patients' suffering from enteric fever would posses antibodies in their sera which can react and agglutinate serial doubling dilutions of killed, coloured salmonella antigens in a tube agglutination test.

17 A. Rapid screening test 1)Place 2 drops of patient's serum. 2)Add 1 drop of the suspension (S.typhi and S.paratyphi,A and B for H and O antigen ). 3)Mix and read within 1 minute.

18 B. tube test, also known as a culture tube or sample tube, is a common piece of laboratory glassware consisting of a finger-like length of glass or clear plastic tubing, open at the top, usually with a rounded U-shaped bottom. Tube agglutination test fordetermining antibody titer

19 The procedure is done as follows : 1)Dilute patient serum in normal saline (1\20,1\40,1\80 1\160,1\320,1\640,1\1280). 2)Add 1 drop of the suspension. 3)Mix and incubate (O antigen at 50 C for 4hrs,while H antigen at 50 C for 2hrs). 4)Examine for agglutination at the bottom of the tube.

20 c.rapid slide titration Similar to rapid screening test,but known quantities of serum are added (e.g. 80 ul, 40 ul, 20 ul, 10 ul, 5 ul, 1.0 give dilution of 1\20, 1\40, 1\80, 1\160, 1\320 respectively).

21 Aseel Abd AL-Razaq

22 Enzyme Linked Immunosorbent Assay (ELISA) has become one of the most widely used serological tests for antibody or antigen detection. ELISA include: Direct and Indirect immunosorbent assay. The direct ELISA is used to detect antigens against a specific antibody bound in a test well. The indirect ELISA is used to detect antibodies against an antigen bound in a test well. ELISA techniques use antibodies linked to an enzyme and Antigen- antibody reactions are detected by enzyme activity.

23 Direct ELISA Direct ELISA for antigen detection. A common use of the direct ELISA test is to detect the presence of drugs in urine. For this test,antibodies specific for the drug are adsorbed to the well on the microtiter plate. When the patient's urine sample is added to the well, any of the drug that it contained would bind to the antibody and is captured. The well is rinsed to remove any unbound drug.

24 To make a visible test, more antibodies specific to the drug are now added (these antibodies have an enzyme attached to them- therefore, the term enzyme-linked ) and will react with the already-captured drug, forming a "sandwich" of antibody/drug/enzyme-linked antibody This positive test can be detected by adding a substrate for the linked enzyme; a visible color is produced by the enzyme reacting with its substrate and then we measure by spectrophotometry.

25 Direct ELISA

26

27 ELISA Intensity of color correspond to concentration of antibody.

28 Indirect ELISA The indirect ELISA test, detects antibodies in a patient's sample rather than an antigen Indirect ELI SA tests are used, for example, to screen blood for antibodies to HIV.For such a purpose, the microtiter well contains an antigen, such as the inactivated virus that causes the disease the test is designed to diagnose,

29 A sample of the patient's blood is added to the well; if it contains antibodies against the virus, they will react with the it. The well is rinsed to remove unbound antibodies, If antibodies in the blood and the virus in the well have attached to each other, they will remain in the well-a positive test,

30 To make a positive test visible, some anti-h ISG (an immunoglobulin that will attach to any antibody, including the one in the patient's serum that has attached to the virus in the well)is added, The anti-hisg is linked to an enzyme, A positive test consists of a "sandwich" or a virus/antibody/enzyme-linked-anti-hisg, At this point, the substrate for the enzyme is added, and a positive test is detected by the color change caused by the enzyme linked to the anti-hisg.

31 Indirect ELISA

32

33 Thank You

Topic: Serological reactions: the purpose and a principle of reactions. Agglutination test. Precipitation test. CFT, IFT, ELISA, RIA.

Topic: Serological reactions: the purpose and a principle of reactions. Agglutination test. Precipitation test. CFT, IFT, ELISA, RIA. Topic: Serological reactions: the purpose and a principle of reactions. Agglutination test. Precipitation test. CFT, IFT, ELISA, RIA. Serology is the study and use of immunological tests to diagnose and

More information

Basic Immunologic Procedures. Complex Serological Tests

Basic Immunologic Procedures. Complex Serological Tests Basic Immunologic Procedures Complex Serological Tests Amal Alghamdi 2014-2015 1 Classification of antigen-antibody interactions: 1. Primary serological tests: (Marker techniques) e.g. Enzyme linked immuonosorben

More information

Chapter 6: Antigen-Antibody Interactions

Chapter 6: Antigen-Antibody Interactions Chapter 6: Antigen-Antibody Interactions I. Strength of Ag-Ab interactions A. Antibody Affinity - strength of total noncovalent interactions between single Ag-binding site on an Ab and a single epitope

More information

Chapter 18: Applications of Immunology

Chapter 18: Applications of Immunology Chapter 18: Applications of Immunology 1. Vaccinations 2. Monoclonal vs Polyclonal Ab 3. Diagnostic Immunology 1. Vaccinations What is Vaccination? A method of inducing artificial immunity by exposing

More information

LAB 1 - Direct agglutination. Serology-the study of the in vitro reactions between antibody and antigen

LAB 1 - Direct agglutination. Serology-the study of the in vitro reactions between antibody and antigen LAB 1 - Direct agglutination Serology-the study of the in vitro reactions between antibody and antigen Serological reaction: - quantitative (weight/volume) - qualitative Agglutination - the aggregation

More information

Chapter 6. Antigen-Antibody Properties 10/3/2012. Antigen-Antibody Interactions: Principles and Applications. Precipitin reactions

Chapter 6. Antigen-Antibody Properties 10/3/2012. Antigen-Antibody Interactions: Principles and Applications. Precipitin reactions Chapter 6 Antigen-Antibody Interactions: Principles and Applications Antigen-Antibody Properties You must remember antibody affinity (single) VS avidity (multiple) High affinity: bound tightly and longer!

More information

Antigens & Antibodies II. Polyclonal antibodies vs Monoclonal antibodies

Antigens & Antibodies II. Polyclonal antibodies vs Monoclonal antibodies A Brief Review of Antibody Structure A Brief Review of Antibody Structure The basic antibody is a dimer of dimer (2 heavy chain-light chain pairs) composed of repeats of a single structural unit known

More information

LAB 14 ENZYME LINKED IMMUNOSORBENT ASSAY (ELISA)

LAB 14 ENZYME LINKED IMMUNOSORBENT ASSAY (ELISA) STUDENT GUIDE LAB 14 ENZYME LINKED IMMUNOSORBENT ASSAY (ELISA) GOAL The goal of this laboratory lesson is to explain the concepts and technique of enzyme linked immunosorbent assay (ELISA). OBJECTIVES

More information

ELISA BIO 110 Lab 1. Immunity and Disease

ELISA BIO 110 Lab 1. Immunity and Disease ELISA BIO 110 Lab 1 Immunity and Disease Introduction The principal role of the mammalian immune response is to contain infectious disease agents. This response is mediated by several cellular and molecular

More information

How Does a Doctor Test for AIDS?

How Does a Doctor Test for AIDS? Edvo-Kit #S-70 How Does a Doctor Test for AIDS? S-70 Experiment Objective: The Human Immunodefi ciency Virus (HIV) is an infectious agent that causes Acquired Immunodefi ciency Syndrome (AIDS) in humans.

More information

HiPer RA Test Teaching Kit

HiPer RA Test Teaching Kit HiPer RA Test Teaching Kit Product Code: HTI019 Number of experiments that can be performed: 20 Duration of Experiment: 1hour Storage Instructions: The kit is stable for 6 months from the date of receipt

More information

CHAPTER 2 ANTIGEN/ANTIBODY INTERACTIONS

CHAPTER 2 ANTIGEN/ANTIBODY INTERACTIONS CHAPTER 2 ANTIGEN/ANTIBODY INTERACTIONS See APPENDIX (1) THE PRECIPITIN CURVE; (2) LABELING OF ANTIBODIES The defining characteristic of HUMORAL immune responses (which distinguishes them from CELL-MEDIATED

More information

Comparing the Tube and Gel Techniques for ABO Antibody Titration, as Performed in Three European Centers 林 良 豐 100.07.18

Comparing the Tube and Gel Techniques for ABO Antibody Titration, as Performed in Three European Centers 林 良 豐 100.07.18 Comparing the Tube and Gel Techniques for ABO Antibody Titration, as Performed in Three European Centers 林 良 豐 100.07.18 Introduction Data from 60 consecutive ABO-incompatible kidney transplantations performed

More information

Serology: Fluorescent antibody tests and other tests employing conjugated antibodies

Serology: Fluorescent antibody tests and other tests employing conjugated antibodies Serology: Fluorescent antibody tests and other tests employing conjugated antibodies Authors: Adapted by Prof M van Vuuren. Originally compiled by Dr RW Worthington. (Retired) Licensed under a Creative

More information

IV. Principles of Serological Testing in Immunohematology

IV. Principles of Serological Testing in Immunohematology IV. Principles of Serological Testing in Immunohematology A. Blood Group Antigens and Antibodies 1. Blood group antigens are structures embedded in or protruding from RBCs, WBCs and Platelets. 2. Exposure

More information

Cold Agglutination Titer detecting Cold Reacting Antibodies

Cold Agglutination Titer detecting Cold Reacting Antibodies Objectives: Cold Agglutination Titer detecting Cold Reacting Antibodies 1. Perform a serial dilution to determine the amount of cold reacting antibody present in a patient specimen with the results obtained

More information

Direct Antiglobulin Test (DAT)

Direct Antiglobulin Test (DAT) Exercise 8 Exercise 9 Direct Antiglobulin Test (DAT) Elution Study Task Aim Introduction To perform the DAT and elution procedure with correct interpretation of results. To perform with 100% accuracy the

More information

Objectives. Immunologic Methods. Objectives. Immunology vs. Serology. Cross Reactivity. Sensitivity and Specificity. Definitions

Objectives. Immunologic Methods. Objectives. Immunology vs. Serology. Cross Reactivity. Sensitivity and Specificity. Definitions Immunologic Methods Part One Definitions Part Two Antigen-Antibody Reactions CLS 420 Clinical Immunology and Molecular Diagnostics Kathy Trudell MLS (ASCP) CM SBB CM ktrudell@nebraskamed.com Discuss the

More information

Direct Testing Systems and Serology

Direct Testing Systems and Serology Direct Testing Systems and Serology Rapid Manual Tests 6-2 Serology Diagnostics 6-6 BD Diagnostics Diagnostic Systems Catalog 2005/2006 6-1 Rapid Manual Tests Meningitis Test Systems 252360 Directigen

More information

Direct Antiglobulin Test (DAT)

Direct Antiglobulin Test (DAT) Exercise 8 Direct Antiglobulin Test (DAT) Objectives: 1. State the purpose for performing the DAT. 2. State what a positive DAT indicates. 3. List the reagents which are used for performing the DAT. 4.

More information

www.gbo.com/bioscience Tissue Culture 1 Cell/ Microplates 2 HTS- 3 Immunology/ HLA 4 Microbiology/ Bacteriology Purpose Beakers 5 Tubes/Multi-

www.gbo.com/bioscience Tissue Culture 1 Cell/ Microplates 2 HTS- 3 Immunology/ HLA 4 Microbiology/ Bacteriology Purpose Beakers 5 Tubes/Multi- 11 Cryo 5 Tubes/Multi 2 HTS 3 Immunology / Immunology Technical Information 3 I 2 96 Well ELISA 3 I 4 96 Well ELISA Strip Plates 3 I 6 8 Well Strip Plates 3 I 7 12 Well Strip Plates 3 I 8 16 Well Strip

More information

IgM ELISA. For the quantitative determination of IgM in human serum and plasma. For Research Use Only. Not For Use In Diagnostic Procedures.

IgM ELISA. For the quantitative determination of IgM in human serum and plasma. For Research Use Only. Not For Use In Diagnostic Procedures. IgM ELISA For the quantitative determination of IgM in human serum and plasma For Research Use Only. Not For Use In Diagnostic Procedures. Please read carefully due to Critical Changes, e.g., Calibrator

More information

Serotyping Techniques

Serotyping Techniques Serotyping Techniques Thomas A. Kruzel, M.T., N. D. Southwest College of Naturopathic Medicine & Health Sciences ABO Blood Groups Blood Group RBC Antigens Serum Antibodies Percentage O none Anti A & B

More information

Blood Typing Laboratory Exercise 40

Blood Typing Laboratory Exercise 40 Blood Typing Laboratory Exercise 40 Background Blood typing involves identifying protein substances called antigens that are present in red blood cell membranes. Although there are many different antigens

More information

chapter 6 Antigen-Antibody Interactions: Principles and Applications Strength of Antigen-Antibody Interactions

chapter 6 Antigen-Antibody Interactions: Principles and Applications Strength of Antigen-Antibody Interactions 8536d_ch06_137-160 8/1/02 12:41 PM Page 137 mac79 Mac 79:45_BW:Goldsby et al. / Immunology 5e: Antigen-Antibody Interactions: Principles and Applications chapter 6 THE ANTIGEN-ANTIBODY INTERACTION IS A

More information

Anti-Treponema Syphilis IgM. Cat # 1464Z

Anti-Treponema Syphilis IgM. Cat # 1464Z DIAGNOSTIC AUTOMATION, INC. 23961 Craftsman Road, Suite D/E/F, Calabasas, CA 91302 Tel: (818) 591-3030 Fax: (818) 591-8383 onestep@rapidtest.com technicalsupport@rapidtest.com www.rapidtest.com See external

More information

QUICK REFERENCE TO BLOOD BANK TESTING

QUICK REFERENCE TO BLOOD BANK TESTING QUICK REFERENCE TO BLOOD BANK TESTING All Blood bank Tests are performed on demand 24 hours a day, 7 days a week. Feto/Maternal Bleed Quantitation estimates will be available within 4 hours of blood bank

More information

AGGLUTINATION MODULE 60.1 INTRODUCTION OBJECTIVES. Notes

AGGLUTINATION MODULE 60.1 INTRODUCTION OBJECTIVES. Notes MODULE Agglutination 60 AGGLUTINATION 60.1 INTRODUCTION Agglutination is one of the antigen and specific antibody reactions which takes place when the two are mixed in-vitro in laboratory in the presence

More information

Introduction. Laboratory Procedure Manual

Introduction. Laboratory Procedure Manual Exercise 9 Elution Study Objectives: 1. State when an elution procedure may be performed. 2. List four situations in which the performance of an elution may provide helpful information. 3. List three types

More information

Blood-Based Cancer Diagnostics

Blood-Based Cancer Diagnostics The Biotechnology Education Company Blood-Based Cancer Diagnostics EDVO-Kit 141 Store entire experiment at room temperature. EXPERIMENT OBJECTIVE: The objective of this experiment is to learn and understand

More information

IgE (Human) ELISA Kit

IgE (Human) ELISA Kit IgE (Human) ELISA Kit Catalog Number KA0216 96 assays Version: 03 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Intended Use... 3 Background... 3 Principle of the Assay...

More information

Veterinary Testing. Classes of Test

Veterinary Testing. Classes of Test Veterinary Testing Classes of Test July 2014 Copyright National Association of Testing Authorities, Australia 2014 This publication is protected by copyright under the Commonwealth of Australia Copyright

More information

Medical Microbiology

Medical Microbiology 1. MED. MICROBI0L.-VOL 16 (1983). 111-116 6 1983 The Pathological Society of Great Britain and Ireland The Journal of Medical Microbiology Vol. 16, No. 2 COMPARISON OF ENZYME IMMUNOASSAY WITH RADIOIMMUNOASSAY

More information

Immunoglobulin E (IgE) concentrations in Human. Immunoglobulin E (IgE) Human ELISA Kit

Immunoglobulin E (IgE) concentrations in Human. Immunoglobulin E (IgE) Human ELISA Kit ab108650 Immunoglobulin E (IgE) Human ELISA Kit Instructions for Use For the quantitative measurement of Immunoglobulin E (IgE) concentrations in Human serum. This product is for research use only and

More information

an antirubella antibody labelled with iodine-125 not require purified rubella antigen and, in general, are resistant to false positive results due to

an antirubella antibody labelled with iodine-125 not require purified rubella antigen and, in general, are resistant to false positive results due to J Clin Pathol 1985;38:1150-1154 Public Health Laboratory Service IgM antibody capture enzyme linked immunosorbent assay for detecting rubella specific IgM KATHRYN BELLAMY,* J HODGSON,t PS GARDNER,* P MORGAN-CAPNERt

More information

VETERINARY SERVICES MEMORANDUM NO. 800.90

VETERINARY SERVICES MEMORANDUM NO. 800.90 August 5, 1998 VETERINARY SERVICES MEMORANDUM NO. 800.90 Subject: To: Guidelines for Veterinary Biological Relative Potency Assays and Reference Preparations Based on ELISA Antigen Quantification Veterinary

More information

Instructions for use of 1 % Sheep-Erythrocytes-Suspension

Instructions for use of 1 % Sheep-Erythrocytes-Suspension Instructions for use of 1 % Sheep-Erythrocytes-Suspension Ready to use for CFT Order No.: E-420, E-423, E-430, E-433, E-450, E-453 Diagnostic Importance The Complement Fixation Test (CFT) is a classical

More information

Chapter 3. Immunity and how vaccines work

Chapter 3. Immunity and how vaccines work Chapter 3 Immunity and how vaccines work 3.1 Objectives: To understand and describe the immune system and how vaccines produce immunity To understand the differences between Passive and Active immunity

More information

Anti-Treponema Syphilis IgM Cat # 1464Z

Anti-Treponema Syphilis IgM Cat # 1464Z DIAGNOSTIC AUTOMATION, INC. 23961 Craftsman Road, Suite E/F, Calabasas, CA 91302 Tel: (818) 591-3030 Fax: (818) 591-8383 onestep@rapidtest.com technicalsupport@rapidtest.com www.rapidtest.com See external

More information

Blood Stains at the Crime Scene Forensic Investigation

Blood Stains at the Crime Scene Forensic Investigation Blood Stains at the Crime Scene Forensic Investigation Introduction Blood stains at a crime scene can be crucial in solving the crime. Numerous analytical techniques can be used to study blood stains.

More information

RHESUS BLOOD GROUP SYSTEM

RHESUS BLOOD GROUP SYSTEM MODULE Rhesus Blood Group System 12 RHESUS BLOOD GROUP SYSTEM 12.1 INTRODUCTION The Rhesus blood group system is the second most important system in transfusion practice. Rh typing is routinely performed

More information

Anti-Zona Pellucida Antibody Latex Agglutination Test

Anti-Zona Pellucida Antibody Latex Agglutination Test Instructions for use Anti-Zona Pellucida Antibody Latex Agglutination Test Cat. No.: BS-20-10 Size: 50 Determinations Storage: 2 C 8 C (36 F 46 F) Screening test for the determination of anti-zona pellucida

More information

MYCOLOGY PARASITOLOGY RHEUMATOID FACTORS VIROLOGY SAMPLE COLLECTION

MYCOLOGY PARASITOLOGY RHEUMATOID FACTORS VIROLOGY SAMPLE COLLECTION C A T A L O G U E C L I N I C A L D I A G N O S T I C S couv BACTERIOLOGYOGY MYCOLOGY PARASITOLOGY RHEUMATOID FACTORS VIROLOGY SAMPLE COLLECTION couv EXPORT DEPARTMENT Orders, technical and commercial

More information

Rapid Screening Tests

Rapid Screening Tests Rapid Screening Tests Infectious Diseases Cardiac Marker Tumor Markers Pregnancy Rheumatology Allergy Drugs of Abuse > For rapid and cost-effective diagnosis > Accurate, reliable results > Easy to use

More information

HiPer Blood Grouping Teaching Kit

HiPer Blood Grouping Teaching Kit HiPer Blood Grouping Teaching Kit Product Code: HTI008 Number of experiments that can be performed: 100 Duration of Experiment: Protocol: 15 minutes Storage Instructions: The kit is stable for 6 months

More information

This module is for INFORMATION ONLY. No BETC test questions are from this module. On completion of this module, participants will be able to:

This module is for INFORMATION ONLY. No BETC test questions are from this module. On completion of this module, participants will be able to: MODULE 3 - LABORATORY TESTING Objective This module is for INFORMATION ONLY. No BETC test questions are from this module. On completion of this module, participants will be able to: o o Identify the types

More information

QuickTiter FeLV Core Antigen ELISA Kit (FeLV p27)

QuickTiter FeLV Core Antigen ELISA Kit (FeLV p27) Product Manual QuickTiter FeLV Core Antigen ELISA Kit (FeLV p27) Catalog Numbers VPK-155 96 wells FOR RESEARCH USE ONLY Not for use in diagnostic procedures Introduction Feline leukemia virus (FeLV) is

More information

Lymphatic System - Structures

Lymphatic System - Structures Lymphatic System - Structures Lymphatic vessels Lymphatic tissues and organs 1 Lymphatic System - Functions Returns leaked plasma to blood vessels Cleanses lymph of bacteria & other foreign matter Provides

More information

Mouse C-Peptide I EIA

Mouse C-Peptide I EIA KAMIYA BIOMEDICAL COMPANY Mouse C-Peptide I EIA For the quantitative determination of C-Peptide I in mouse plasma, serum and urine. Cat. No. KT-367 For Research Use Only. 1 Rev. 091207 PRODUCT INFORMATION

More information

P R O D U C T S CATALOG 2011-2012

P R O D U C T S CATALOG 2011-2012 P R O D U C T S CATALOG 2011-2012 INSTITUTE FOR THE APPLICATION OF NUCLEAR ENERGY - INEP Banatska 31b 11080 Zemun Belgrade Serbia Tel: (+381 11) 2619 252, 2618 696, 2199 949 Fax: (+381 11) 2618 724 www.inep.co.rs

More information

4A. Types of Laboratory Tests Available and Specimens Required. Three main types of laboratory tests are used for diagnosing CHIK: virus

4A. Types of Laboratory Tests Available and Specimens Required. Three main types of laboratory tests are used for diagnosing CHIK: virus 4. LABORATORY 4A. Types of Laboratory Tests Available and Specimens Required Three main types of laboratory tests are used for diagnosing CHIK: virus isolation, reverse transcriptase-polymerase chain reaction

More information

9- Labeled Immunoassays Fluorescent & Chemiluminescent Immunoassays

9- Labeled Immunoassays Fluorescent & Chemiluminescent Immunoassays 9- Labeled Immunoassays Fluorescent & Chemiluminescent Immunoassays In 1944 it was demonstrated that antibodies could be labeled with molecules that fluoresce. These fluorescent compounds are called fluorophores

More information

Mouse IgM ELISA. Cat. No. KT-407 K-ASSAY. For the quantitative determination of IgM in mouse biological samples. For Research Use Only. 1 Rev.

Mouse IgM ELISA. Cat. No. KT-407 K-ASSAY. For the quantitative determination of IgM in mouse biological samples. For Research Use Only. 1 Rev. K-ASSAY Mouse IgM ELISA For the quantitative determination of IgM in mouse biological samples Cat. No. KT-407 For Research Use Only. 1 Rev. 072309 K-ASSAY PRODUCT INFORMATION Mouse IgM ELISA Cat. No. KT-407

More information

QuickTiter Hepatitis B Surface Antigen (HBsAg) ELISA Kit

QuickTiter Hepatitis B Surface Antigen (HBsAg) ELISA Kit Product Manual QuickTiter Hepatitis B Surface Antigen (HBsAg) ELISA Kit Catalog Numbers VPK-5004 VPK-5004-5 96 assays 5 x 96 assays FOR RESEARCH USE ONLY Not for use in diagnostic procedures Introduction

More information

Direct Antiglobulin Test (DAT)

Direct Antiglobulin Test (DAT) Exercise 8 Exercise 9 Task Direct Antiglobulin Test (DAT) Elution Study To perform the DAT and elution procedure with correct interpretation of results. Obectives 1. State what the Direct Antiglobulin

More information

What Forensics Information Does Blood Typing Provide?

What Forensics Information Does Blood Typing Provide? The Biotechnology Education Company EDVO-Kit 191 What Forensics Information Does Blood Typing Provide? See Page 3 for storage instructions. EXPERIMENT OBJECTIVE: The objective of this experiment is to

More information

www.gbo.com/bioscience Tissue Culture 1 Cell/ Microplates 2 HTS- 3 Immunology/ HLA 4 Microbiology/ Bacteriology Purpose Beakers 5 Tubes/Multi-

www.gbo.com/bioscience Tissue Culture 1 Cell/ Microplates 2 HTS- 3 Immunology/ HLA 4 Microbiology/ Bacteriology Purpose Beakers 5 Tubes/Multi- 11 Cryo 5 Tubes/Multi 2 HTS 3 Immunology / Immunology Technical Information 3 I 2 96 Well ELISA 3 I 4 96 Well ELISA Strip Plates 3 I 6 8 Well Strip Plates 3 I 7 12 Well Strip Plates 3 I 8 16 Well Strip

More information

IgM ANTIBODY CAPTURE ENZYME-LINKED IMMUNOSORBENT ASSAY PROTOCOL

IgM ANTIBODY CAPTURE ENZYME-LINKED IMMUNOSORBENT ASSAY PROTOCOL Appendix C. IgM and IgG Serologic Assay Protocols IgM ANTIBODY CAPTURE ENZYME-LINKED IMMUNOSORBENT ASSAY PROTOCOL Introduction Principle Safety Assays that detect viral specific immunoglobulin M (IgM)

More information

Laboratory Procedure Handout. Detection of Anti-streptolysin O (ASL)

Laboratory Procedure Handout. Detection of Anti-streptolysin O (ASL) KING ABDULAZIZ UNIVERSITY FACULTY OF MEDICINE & ALLIED HEALTH MEDICAL TECHNOLOGY PROGRAM Laboratory Procedure Handout Detection of Anti-streptolysin O (ASL) Latex agglutination Assay for the detection

More information

RhD typing. Practice for IV year medical students. Zita Csernus MD. National Blood Transfusion Service Blood Transfusion Centre Pécs

RhD typing. Practice for IV year medical students. Zita Csernus MD. National Blood Transfusion Service Blood Transfusion Centre Pécs immunisation Bed side test Antibody tests RhD typing Practice for IV year medical students Zita Csernus MD National Blood Transfusion Service Blood Transfusion Centre Pécs Rh Blood Group System Discovery:

More information

Development of slide agglutination test for the rapid diagnosis of Mycoplasma infection in the chicken

Development of slide agglutination test for the rapid diagnosis of Mycoplasma infection in the chicken The Bangladesh Veterinarian (2011) 28(2) : 80 84 Development of slide agglutination test for the rapid diagnosis of Mycoplasma infection in the chicken M. Arefin, J. A. Begum, R. Parvin, M. M. Rahman,

More information

Immunohematology. Primary vs. Secondary Response. Blood Group Antibodies. What do we do with that tube of blood?

Immunohematology. Primary vs. Secondary Response. Blood Group Antibodies. What do we do with that tube of blood? Immunohematology Jeffrey S. Jhang, MD Assistant Director, Transfusion Medicine Blood groups antigens Markers on red cell structures Detected by serologic techniques Multiple alleles within each system

More information

Blood Type Testing Lab Report Section 1101 Nattanit Trakullapphan (Nam) Chawalnrath Wongdeshanan (Kat)

Blood Type Testing Lab Report Section 1101 Nattanit Trakullapphan (Nam) Chawalnrath Wongdeshanan (Kat) Blood Type Testing Lab Report Section 1101 Nattanit Trakullapphan (Nam) Chawalnrath Wongdeshanan (Kat) Introduction: figure 1.1 (Blood type n.d.) figure 1.2 (Blood type, Antigens-Antibodies n.d.) Multiple

More information

Laboratory Procedures. Serological detection of avian influenza A(H7N9) virus infections by turkey haemagglutinationinhibition

Laboratory Procedures. Serological detection of avian influenza A(H7N9) virus infections by turkey haemagglutinationinhibition Laboratory Procedures Serological detection of avian influenza A(H7N9) virus infections by turkey haemagglutinationinhibition assay 23 May 2013 The WHO Collaborating Center for Reference and Research on

More information

serology Agglutination Techniques and Blood Cell Identification

serology Agglutination Techniques and Blood Cell Identification Serology: Agglutination Techniques and Blood Cell Identification S erology is a branch of immunology dealing with techniques to identify and measure antigens, and to detect serum antibodies. Agglutination

More information

Laboratory Procedure Manual

Laboratory Procedure Manual Exercise 3 Textbook: ABO and D Typing Blaney, Chapter 4 The ABO and H Blood Group Systems and 5 Rh Blood Group System Skills: 20 points Objectives 1. State the reason why ABO blood groups are the most

More information

Mouse Creatine Kinase MB isoenzyme (CKMB) ELISA

Mouse Creatine Kinase MB isoenzyme (CKMB) ELISA KAMIYA BIOMEDICAL COMPANY Mouse Creatine Kinase MB isoenzyme (CKMB) ELISA For the quantitative determination of mouse CKMB in serum, plasma, cell culture fluid and other biological fluids Cat. No. KT-57681

More information

Dengue Virus Serology Testing

Dengue Virus Serology Testing Dengue Virus Serology Testing 40% of the world s population is at risk of dengue infection Focus Diagnostics has been a leader in the development of infectious disease immunology assays for more than 25

More information

Helicobacter pylori IgG ELISA Kit

Helicobacter pylori IgG ELISA Kit Helicobacter pylori IgG ELISA Kit Catalog Number KA0219 96 assays Version: 02 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Intended Use... 3 Background... 3 Principle

More information

Herpes-simplex virus-1 and/or -2 IgM ELISA Kit

Herpes-simplex virus-1 and/or -2 IgM ELISA Kit Herpes-simplex virus-1 and/or -2 IgM ELISA Kit Qualitative assays for anti-herpes simplex IgM antibodies For Research Use Only. Not for use in diagnostic procedures. Changes from previous version 04/18/02

More information

Basics of Immunology

Basics of Immunology Basics of Immunology 2 Basics of Immunology What is the immune system? Biological mechanism for identifying and destroying pathogens within a larger organism. Pathogens: agents that cause disease Bacteria,

More information

Laboratory diagnosis of viral infections. Specimen collection and transport

Laboratory diagnosis of viral infections. Specimen collection and transport Laboratory diagnosis of viral infections Specimen collection and transport Steps in Pathogenesis of Viral Diseases 1. Entry through skin through mucosa (respiratory, gastrointestinal or genital) through

More information

WHO Prequalification of Diagnostics Programme PUBLIC REPORT. Product: Genscreen ULTRA HIV Ag-Ab Number: PQDx 0096-031-00. Abstract

WHO Prequalification of Diagnostics Programme PUBLIC REPORT. Product: Genscreen ULTRA HIV Ag-Ab Number: PQDx 0096-031-00. Abstract WHO Prequalification of Diagnostics Programme PUBLIC REPORT Product: Genscreen ULTRA HIV Ag-Ab Number: PQDx 0096-031-00 Abstract Genscreen ULTRA HIV Ag-Ab with product codes 72386 and 72388, manufactured

More information

TECHNICAL UNIVERSITY OF MOMBASA

TECHNICAL UNIVERSITY OF MOMBASA TECHNICAL UNIVERSITY OF MOMBASA Faculty of Applied and Health Sciences DEPARTMENT OF MEDICAL SCIENCES DIPLOMA IN MEDICAL LABORATORY SCIENCES (DMLS 13 J MID / DMLS 12M ) AML 2222 : IMMUNOLOGY II SPECIAL/SUPPLEMENTARY:

More information

Zika Virus. Fred A. Lopez, MD, MACP Richard Vial Professor Department of Medicine Section of Infectious Diseases

Zika Virus. Fred A. Lopez, MD, MACP Richard Vial Professor Department of Medicine Section of Infectious Diseases Zika Virus Fred A. Lopez, MD, MACP Richard Vial Professor Department of Medicine Section of Infectious Diseases What is the incubation period for Zika virus infection? Unknown but likely to be several

More information

The Blood Group Systems. Inheritance and Genetics

The Blood Group Systems. Inheritance and Genetics The Blood Group Systems Inheritance and Genetics History of Blood Groups and Blood Transfusions Experiments with blood transfusions have been carried out for hundreds of years. Many patients have died

More information

Dengue IgM ELISA. For the quantitative determination of IgM-class antibodies to Dengue Virus in serum.

Dengue IgM ELISA. For the quantitative determination of IgM-class antibodies to Dengue Virus in serum. Dengue IgM ELISA For the quantitative determination of IgM-class antibodies to Dengue Virus in serum. For Research Use Only. Not For Use In Diagnostic Procedures. Catalog Number: 20-DEMHU-E01 Size: 96

More information

Appendix B: Provincial Case Definitions for Reportable Diseases

Appendix B: Provincial Case Definitions for Reportable Diseases Infectious Diseases Protocol Appendix B: Provincial Case Definitions for Reportable Diseases Disease: Lassa Fever Revised December 2014 Lassa Fever 1.0 Provincial Reporting Confirmed and probable cases

More information

Testing for RA. The Ideal Lab Test. William M. Wason, MD, PhD 9/24/2010. Confusion Abounds

Testing for RA. The Ideal Lab Test. William M. Wason, MD, PhD 9/24/2010. Confusion Abounds Confusion Abounds Rheumatoid arthritis: ulnar deviation and muscle artrophy, hands Poor sensitivity and specificity Hepatitis C causes lots of false + tests Changing technology in how tests are done Historic

More information

Artificial Blood Typing

Artificial Blood Typing Artificial Blood Typing Background: Human blood may be classified according to the presence or absence of certain antigens or factors, that are attached to the surface of the red blood cells, or erythrocytes.

More information

Helicobacter pylori IgG ELISA Kit

Helicobacter pylori IgG ELISA Kit Helicobacter pylori IgG ELISA Kit Catalog Number KA0220 96 assays Version: 02 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Intended Use... 3 Background... 3 Principle

More information

1) Siderophores are bacterial proteins that compete with animal A) Antibodies. B) Red blood cells. C) Transferrin. D) White blood cells. E) Receptors.

1) Siderophores are bacterial proteins that compete with animal A) Antibodies. B) Red blood cells. C) Transferrin. D) White blood cells. E) Receptors. Prof. Lester s BIOL 210 Practice Exam 4 (There is no answer key. Please do not email or ask me for answers.) Chapters 15, 16, 17, 19, HIV/AIDS, TB, Quorum Sensing 1) Siderophores are bacterial proteins

More information

VI. ABO and H Blood Groups

VI. ABO and H Blood Groups MLAB 243 4 A. History of ABO System. Discovered in 900 by Karl Landsteiner and remains the most important of the blood group systems as far as the transfusion of blood is concerned. 2. Landsteiner tested

More information

CORD BLOOD EVALUATION

CORD BLOOD EVALUATION CORD BLOOD EVALUATION Principle: When there is incompatibility between a mother s antibodies and an infant s red blood cell antigens, the infant is at risk of developing Hemolytic Disease of the Fetus

More information

Lyme (IgG and IgM) Antibody Confirmation

Lyme (IgG and IgM) Antibody Confirmation Pathology & Laboratory Medicine Lyme (IgG and IgM) Antibody Confirmation TEST UPDATE: New Test Notification Date: 1/9/2013 Effective Date: 1/7/2013 CONTACT INFO Call 802-847-5121 800-991-2799 email labmarketing@vtmednet.org

More information

SOUTH SHORE HEALTH LABORATORY SERVICES STANDARD OPERATING PROCEDURES

SOUTH SHORE HEALTH LABORATORY SERVICES STANDARD OPERATING PROCEDURES APPROVED BY: MEDICAL DIRECTOR LABORATORY MANAGER NAME AND SIGNATURE DR. CHRIS NAUGLER: DEBBIE MOSHER: DATE SUPERCEDES: S25.7.026 REVIEWED BY: NAME SIGNATURE DATE: TRACY MACKAY DATE REMOVED FROM SERVICE:

More information

ELISA. Enzyme immunoassay for the qualitative and quantitative detection of IgM antibodies against. Hepatitis A-Virus.

ELISA. Enzyme immunoassay for the qualitative and quantitative detection of IgM antibodies against. Hepatitis A-Virus. This version US RUO E11 070212 Version 2 of IFU is to be used only for reference purposes. Contents especially technical, methodical and regulations data, may not be amended. Anti-HAV-IgM ELISA Enzyme

More information

Diagnosis of HIV-1 Infection. Estelle Piwowar-Manning HPTN Central Laboratory The Johns Hopkins University

Diagnosis of HIV-1 Infection. Estelle Piwowar-Manning HPTN Central Laboratory The Johns Hopkins University Diagnosis of HIV-1 Infection Estelle Piwowar-Manning HPTN Central Laboratory The Johns Hopkins University Tests Used to Diagnose HIV-1 Infection HIV antibody (today s topic) HIV p24 antigen HIV DNA HIV

More information

竞 争 性 分 析 Epitope Mapping 实 验 方 法

竞 争 性 分 析 Epitope Mapping 实 验 方 法 竞 争 性 分 析 Epitope Mapping 实 验 方 法 ABSTRACT The simplest way to determine whether two monoclonal antibodies bind to distinct sites on a protein antigen is to carry out a competition assay. The assay can

More information

Case Report: Complement and infection susceptibility Diagnosis of complete deficiency of C2

Case Report: Complement and infection susceptibility Diagnosis of complete deficiency of C2 Diagnosis of complete deficiency of C2 Lennart ruedsson MD/PhD, Section of Microbiology, Immunology and Glycobiology Dept of Laboratory Medicine, Lund University, Lund, he patient was a 14-year-old girl

More information

Results Demographic profile of these children is shown in Table I.

Results Demographic profile of these children is shown in Table I. Prevalence of Antibody to Hepatitis C Virus in Pakistani Thalassaemics by Particle Agglutination Test Utilizing C 200 and C 22-3 Viral Antigen Coated Particles Pages with reference to book, From 269 To

More information

Immunohematology. Immunohematology. Blood Group Antigens

Immunohematology. Immunohematology. Blood Group Antigens Immunohematology Immunohematology Jeffrey S. Jhang, MD Assistant Director, Transfusion Medicine Demonstration of red cell antigen-red cell antibody reactions is the key to immunohematology Combination

More information

ABO-Rh Blood Typing With Synthetic Blood

ABO-Rh Blood Typing With Synthetic Blood 70-0101 ABO-Rh Blood Typing With Synthetic Blood Teacher s Manual World-Class Support for Science & Math The ability to type blood is an invaluable tool in the fields of medicine and criminology. Using

More information

TOTAL PROTEIN FIBRINOGEN

TOTAL PROTEIN FIBRINOGEN UNIT: Proteins 16tproteins.wpd Task Determination of Total Protein, Albumin and Globulins Objectives Upon completion of this exercise, the student will be able to: 1. Explain the ratio of albumin and globulin

More information

Toxoplasma IgA. Cat # 1103-11

Toxoplasma IgA. Cat # 1103-11 DIAGNOSTIC AUTOMATION, INC. 23961 Craftsman Road, Suite D/E/F, Calabasas, CA 91302 Tel: (818) 591-3030 Fax: (818) 591-8383 onestep@rapidtest.com technicalsupport@rapidtest.com www.rapidtest.com See external

More information

MLT 118L Clinical Immunology/Immunohematology Lab

MLT 118L Clinical Immunology/Immunohematology Lab Page 1 of 5 MLT 118L Clinical Immunology/Immunohematology Lab Approval Date: Effective Term: Department: MEDICAL LABORATORY TECHNICIAN Division: Allied Health/Public Safety Units: 1.00 Grading Option:

More information

Hillsborough Community College - Ybor City Campus 1025C Laboratory Exercise 9: Testing Urine for the Presence of Drugs Introduction

Hillsborough Community College - Ybor City Campus 1025C Laboratory Exercise 9: Testing Urine for the Presence of Drugs Introduction Hillsborough Community College - Ybor City Campus 1025C Laboratory Exercise 9: Testing Urine for the Presence of Drugs Introduction Drug testing beyond the health care and criminal justice systems has

More information

HuCAL Custom Monoclonal Antibodies

HuCAL Custom Monoclonal Antibodies HuCAL Custom Monoclonal HuCAL Custom Monoclonal Antibodies Highly Specific, Recombinant Antibodies in 8 Weeks Highly Specific Monoclonal Antibodies in Just 8 Weeks HuCAL PLATINUM (Human Combinatorial Antibody

More information

STANDARD OPERATING PROCEDURE FOR THE DIRECT ANTIGLOBULIN TEST

STANDARD OPERATING PROCEDURE FOR THE DIRECT ANTIGLOBULIN TEST STANDARD OPERATING PROCEDURE FOR THE DIRECT ANTIGLOBULIN TEST TITLE: Standard Operating Procedure for Performing the Direct Antiglobulin Test 1.0 Principle To detect in vivo sensitization of red blood

More information

Standardization and Evaluation of IgA and IgM Gel Column Agglutination for Direct Antiglobulin Testing

Standardization and Evaluation of IgA and IgM Gel Column Agglutination for Direct Antiglobulin Testing Standardization and Evaluation of IgA and IgM Gel Column Agglutination for Direct Antiglobulin Testing ABSTRACT BACKGROUND: Diagnosis of autoimmune hemolytic anemia is commonly confirmed with a positive

More information