WANG Zong2wei 1 MOU Xiao2ling 1 YANG Guo2wei 2 LI Ji2kai 2 LIU Da2sen

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1 2009,23 (5) : Journal of Nuclear Agricultural Sciences 891 : (2009) (1 28 ) (11, ;21, ) :216 1,L 9 (3 4 ),1228 : ( P < 0101), ( P < 0105) ( HDL) (LDL) (CHO) ( P < 0105), ( P < 0101) ; T3, ( GH) 21 ( IGF21) ( P < 0105), GH IGF2 1 GH IGF21 : ; ; ; ; ; ; EFFECT OF DIETARY NUTRIENT LEVELS ON GROWTH PERFORMANCE AND SERUM PARAMETERS FOR NORTHEAST GEESE( 1 28d) WANG Zong2wei 1 MOU Xiao2ling 1 YANG Guo2wei 2 LI Ji2kai 2 LIU Da2sen (1. Animal Nutrition Institute, Northeast Agricultural University, Harbin, Heilongjiang ; 2. Institute of grassland, Academy of agricultural sciences in Heilongjiang provincial, Harbin, Heilongjiang ) Abstract :Two hundred and sixteen northeast geese at 1 d of age were randomly allotted to 9 treatments of 24 birds each using four factors and three levels L 9 (3 4 ) orthogonal design, in order to research effects of different levels of dietary metabolic energy (ME), crude protein ( CP), calcium ( Ca) and non2phytic acid phosphorus (NPP) on performance, serum parameters and hormone levels for northeast geese. The results indicated that dietary (ME) significantly affected average daily gain, average daily feed intake, feed gain ratio ( P < 0101), dietary CP significantly affected feed gain ratio ( P < 0105). Dietary ME levels affected the concentrations of HDL LDL and CHO significantly ( P < 0105), and affected the concentration of uric acid extremely significantly ( P < 0101 ). It has strong positive correlation between Average daily gain ( ADG) and the concentration of GH, ADG and the concentration of IGF21, the concentrations of GH and IGF21. Key words : northeast geese ; metabolic energy ; crude protein ; calcium ; non2phytic acid phosphor ; growth performance ; hormone, ;,(C18 2), [1 ] ;,,, [2 ] ;,,, : : : (19822),,,, E2mail : com. cn : (19612),,,, E2mail : edu. cn

2 [3 6 ], [7 ] 29 56, , 1 28, 1 28, 1 28, ( ), 9, 1 item ingredient 1 (, %) Table 1 Dietary composition and nutritive level (air2dry basis, %) treatment maize soybean meal wheat bran alfalfa powder soybean oil fish meal L2Lys DL2Met NaCl CaHPO Limstone Choline Multi2vitamin Minerals total nutrient level ME(MJΠkg) CP Ca NPP CF Lys Met + Cys :8 ( 3 ) : 80 mg, 70 mg, 0110 mg, Zn 50 mg, 8 mg, 0135 mg ;V A 2000IU, V D3 480IU, V E 312IU, V K3 014 mg, V B1 2 mg,v B mg, V B2 5 mg,v B6 4 mg, 116 mg,55 mg, mg,012 mg,0103 mg CP Ca NPP, Note :CaHPO 4 was replaced by Ca (H 2 PO 4 ) 2 in treatment 8. Provided per kg of dietary :Fe 80 mg,mn 70 mg,se 0110 mg,zn 50 mg,cu 8 mg,i 0135 mg ;V A 2000IU, V D3 480IU, V E 312IU, V K3 014 mg, V B1 2 mg,v B mg, V B2 5 mg,v B6 4 mg,calcium pantothenate 116 mg,nicotinic acid 55 mg,biotin mg,folic acid 012 mg, ethoxy quinoline 0103 mg CP Ca NPP were measured values,other nutrient levels were calculated values ,,, 9 ( ), 4, 6 (metabolic energy, ME) (crude protein,cp) (Ca) (Non2phytic acid phosphor,npp), 3,4 3 ( )L 9 (3 4 ),2,28d ( ) 1-10

3 5 (1 28 ) 893,11,, item 2 Table 2 Orthogonal experimental design table factor ME (MJΠkg) CP( %) Ca ( %) NPP( %) 1 1(1019) 1(16) 1 (018) 1 (013) 2 1(1019) 2(18) 2 (110) 2 (015) 3 1(1019) 3(20) 3 (112) 3 (017) 4 2(1117) 1(16) 2 (110) 3 (017) 5 2(1117) 2(18) 3 (112) 1 (013) 6 2(1117) 3(20) 1 (018) 2 (015) 7 3(1215) 1(16) 3 (112) 2 (015) 8 3(1215) 2(18) 1 (018) 3 (017) 9 3(1215) 3(20) 2 (110) 1 (013) ( P < 0101), ( P = ), ( P > 0105) ; (28d), : > >,, >, ( P <, 0101), ( P > 0105) 4,, : > > >,, ( P < 0101), ( Initial weight, IW),( Final weight,fw), ( P > 0105) (Average daily feed intake,adfi), 212 (Average daily gain, ADG), ( Feed gain ratio, FΠ G) ,,4 HDL : >, 12h > >, HDL 28, ( P = ) ;4 LDL 1, : > > >, 5 ml,,3000 rπmin LDL 0105 ( P = ) ;4 15 min, CHO : > > > CX24, CHO 0105 HDL LDL CHO ( TG) (Uric acid) ( P = ) ; TG : > (RIA) > > ;: (T 3 ) ( T 4 ) ( GH) > > >, ( INS) 21 ( IGF21) T 3 T 4 GH ( P < 0101) INS, IGF21,,FH2408, 114 SAS812 ANOVA Duncan [8 ], (SD) SAS812 CORR ( 3),,4 : > > >, ( P < 0101),, ( P > 0105) ;4 : > > >, ; T 3 : > > > ; T 4 : > > > ; GH : > > > ; INS : > > > ; IGF21 : > > >,

4 Table 3 Effects of ME, CP, Ca and NPP in diets on growth performance of northeast geese during 1 to 28 day of age factor ME(MJΠkg) CP( %) Ca ( %) NPP( %) level IW(g) FW(g) determined indicators ADFI(gΠd) ADG(gΠd) FΠG(gΠg) A A A A AB AB AB A B B B B P < < < R a b b P R a a a a b b P R P R :,( P < 0105), ( P < 0101),R Notes : In the same column of the same factor treatment, different superscripts mean significant difference ( P < 0105), different capital letters mean extremely significant difference ( P < 0101), and R means range. The same as following tables Table 4 Effects of ME, CP, Ca and NPP in diets on related serum parameters of northeast geese at 28 day of age factor ME(MJΠkg) CP( %) Ca ( %) NPP( %) level HDL (mmolπl) LDL (mmolπl) determined indicators CHO(mmolΠL) TG(mmolΠL) UA(mmolΠL) b b b B ab ab ab A a a a A P R b a a P R P R P R

5 5 (1 28 ) 895 factor ME (MJΠkg) CP ( %) Ca ( %) NPP ( %) 5 28 Table 5 Effects of ME, CP, Ca and NPP in diets on related hormone of northeast geese at 28 day of age level T 3 (ngπml) T 4 (ngπml) determination of indicators GH(ngΠml) INS(uIUΠml) - 1 IGF21 (ngπml) b b b ab ab b a a a P R P R P R P R ( P < 0105) T 3 T 4 GH INS IGF21 : T 3 ( r = 0181, P < 0101), GH (r = 0165, P = 0102), IGF21 (r = 0162, P = 0103), GH IGF21 (r = 0179, P = 01002), [13 ],,110 %,,, [7 ],,;, ,, LDL CHO TG 1215 MJΠkg, ;HDL CHO, NRC(1994) MJΠkg [9 ], MJΠkg [10 ], MJΠkg MJΠkg ( ) [11 ],(1985) MJΠkg [9 ],,, ;, 18 %,, % % [4 ] 19 % [5 ],,,,, Summers [13 ] (0155 % 0185 %) (013 % 014 %) LDL HDL CHO TG [15 ], ( MJΠkg) 9 HDL LDL CHO TG ( P > 0105) [16 ], ( MJΠkg) 4

6 HDL LDL CHO TG ( P > 0105) [17 ], ( MJΠkg) 70 TG CHO ( P > 0105), (13138 MJΠkg) HDL LDL (12113 MJΠkg) (10187 MJΠkg) ( P < 0105) CHO TG ( P < 0101) [18 ], HDL LDL CHO,P ,,, 1 28, Kzen [18 ], [19 ], [20 ],,,,, [5 ],, ;,, 313 : ( TRH)2 (T 3 T 4 )2 (), [21 ],0 70 T 3,T 4 T 3, T 4 ( GH),, ; 21 ( IGF2I), GH, [17 ],( MJΠkg) 70 GH IGF21,,, GH, IGF21 [22 ],IGF2I, GH GH, GH IGF21,GH GF21,, [23 ] kg,, (14187 MJΠkg),5,20 d (12139 MJΠkg) (9198 MJΠkg),, 28, ;,0 28,, ( ), 4,0 28 : 1215 MJΠkg, 18 %, 018 %, 015 % : [ 1 ]. [J ].,1999, (4) : 2-3 [ 2 ]. [J ].,1991, (2) :40-41 [ 3 ],,,,. [J ].,1999,12 (2) : [ 4 ]. 0-3

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