# Most limiting amino acid concept...

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1 Review... Proteins are composed of amino acids Amino acids are the essential nutrients The dietary provision of amino acids in correct amount and provisions determines the adequacy of the protein in the diet Review... Most limiting amino acid concept... Def.- the amino acid found in the diet at the lowest concentration relative to the animals requirements Finishing diet formulated to contain 13.2 CP would contain: Finishing diet formulated to contain 13.2 CP would contain:.61 (req. 0.60).61/.60*100 = (req. 0.16).22/.16*100 = (req 0.41).54/.41*100 = (req.11).16/.11*100 = 145 Which AA is most limiting? 1 st limiting = 2 nd limiting = 3 rd limiting = 4 th limiting = Therefore, if you formulated the diet on a basis, the diet would be sufficient in all AA. If the diet was formulated on a basis, it would be deficient in. 1

2 Nutrient Requirements... AA requirements for the growing pig are related to the rate of protein accretion, energy intake, and dietray energy density Growing pigs Daily requirement = Maintenance + Protein accretion Protein:Energy Relationship Protein accretion requires energy Up to a point, protein accretion is linearly correlated with energy intake Animals usually consume enough feed to meet their energy requirements if given ad libitum access to feed Requirement... The requirement can be expressed as a ratio of :Energy AA Requirements... Amino acid requirements can be listed as a ratio of the AA to The requirement ( or g/kg diet ) increases with increasing energy concentration in the diet [ME] diet Feed Intake How much should be in the diet? Body Weight (kg), ME (kcal/kg) 3,265 3,265 3,265 3,265 3,265 3,265 ine ()

3 What if we increase the dietary ME level? Amino acid ratios relative to? Can we adjust other amino acid levels? If so, how, and to what level? Protein Quality... Amino Acid Ratios... Protein Quality increases as the ratio of amino acids in the feedstuffs more closely resembles the ratio of amino acids required by the animal kg kg Corn SBM '70:30 Converting to a basis... Maintaining the :ME? of

4 Converting to a basis... Amino Acid Ratios Relative to of kg Corn SBM '70:30 The Concept of an Ideal Protein Must contain all EAA, and in the correct ratios. The closer the amino acid profile of the dietary protein to that of the tissue or product being produced, the better the biological value and efficiency of utilization. There is a high correlation between AA requirements for growth and the amino acid composition of the carcass. Diet abolism Cell & Tissue Proteins Diet abolism Animal Cells & Tissue EAA Muscle 100 units lys 114 units arg 32 units his 46 units met 66 units phe 56 units tyr EAA 100 units lys 114 units arg 32 units his 46 units met 66 units phe 56 units tyr Muscle 100 units lysine 114 units arg 32 units hist 46 units meth 66 units phen 56 units tyr 4

5 V. The Concept of an Ideal Protein A. Practical application: The requirements for EAA can be calculated if the lysine requirement is known. B. Examples from the NRC Table 1. Maintenance 2. Protein Accretion 3. Lactation Converting to a basis... of Ideal protein concept... VI. Feed ingredients contain various proteins that are digested to provide amino acids. A. Soybean meal - 44 or 48 crude protein 1. Low in sulfur amino acids, but a good source of other amino acids. B. Corn gluten meal 1. A good source of sulfur amino acids. Amino Acid Ratios Relative to Deficits can be covered by blending complimentary ingredients together, or by providing synthetic amino acids kg Corn SBM '70:30 5

6 Concept of Bioavailability... Digestibility... Bioavailability- the availability of a given nutrient for absorption by the animal A given feed ingredient may contain 2 g of /kg, but only 1.5 g may be available to the animal for absorption (Amt. of Nutrient in Feed- Amt of Nutrient in Feces) Amt. of Nutrient in Feed However, digestibility based on fecal amino acid excretion does not accurately represent the the amount or proportion of amino acids digested by the animal. Why not??? Nutrient Requirements Ileal digestibility... Feed In Available (digestible) (Amt. of Nutrient in Feed- Amt of Nutrient in Ileal digesta) Amt. of Nutrient in Feed Ileum Feces Out A more accurate estimate of the amino acid availability to the animal. 6

7 Apparent ileal digestibility... True AA digestibility... [AA] feed [AA] digesta [AA] feed Apparent AA digestibility is adjusted to account for endogenous losses Ileal digesta contains amino acids from two sources: 1. Unabsorbed feed amino acids 2. Amino acids from endogenous origin Sloughed cells Non reabsorbed digestive enzymes [AA] feed [AA] digesta + [Endogenous AA] digesta [AA] feed True AA digestibility... Difficulty in determining True AA digestibility lies in the difficulties associated with determining endogenous AA losses Problem: 1 g lysine consumed in 1000 g feed (1 of diet) 2.1 g ileal lysine remaining, 7.9 g absorbed 0.3 g endogenous ileal lysine Apparent: 1. (10-2.1)/10 = 79 (0.79) True: 2. [10 - ( )]/10 = 82 (.82) 7

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