SAP TESTING PLANT SAMPLING GUIDE
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1 SAP TESTING PLANT SAMPLING GUIDE
2 Hortus Plant Sampling Guide This guide is intended to help ensure that the correct plant part and number of leaves required for a sap test are being collected and sent to our laboratory for testing. This will allow for faster, more accurate testing of your sample, which in turn leads to you receiving your recommendation in the shortest turnaround time. If you require further information please do not hesitate to call us on to speak to one of our friendly, knowledgeable staff.
3 Plant Sampling Select the correct leaf consult sampling guide. For young or small crops a larger sample will be required. Select an average representative area in the field for monitoring. Select average representative plants avoid diseased plants. Collect enough sample for 5ml sap consult manual for leaf numbers. Do not mix varieties in the sample as there may be some variation. Sample from 8am 3pm, but monitor the same time each week. Do not sample immediately after rain note heat or cold stresses. Note fertiliser and irrigation times and application rates if possible.
4 Handling and Transport Use plastic bags. Collect dry samples. Keep cool and dry (don t freeze or sit on dash in direct sun). Send via the fastest form of delivery to minimise time in transit. Apply Keep cool and dry stickers if available. Do not over pack compression will cause deterioration.
5 Analysis Request Form Please fill in all information on the Analysis Request Form (ARF). This can be done online by logging in using your unique user ID (your address and a password). Simply register online to set up your online profile. Please indicate what sort of recommendation you require on the ARF as this will also influence the price you are charged for your results. A detailed ARF gives us a complete understanding of the growing conditions of your crop and lets us know if you are concerned about specific problems or symptoms and allows us to address these in our recommendations. It also ensures we generate the appropriate optimal level for your particular situation. The ARF can be completed online at our preferred and easiest option. You can also download a single hardcopy form from our website or order a carbon copy booklet of 50 forms.
6 Asparagus Take stems from the top 20cm of the plant. Take at least 30 tops, randomly across the sampling area. Begin sampling at fern formation; and monitor monthly to mature fern stage.
7 Aster Take first fully expanded leaf - usually the fourth or fifth leaf back from the growing point of the plant, or out from the crown of the plant. BE SURE TO TAKE WHOLE LEAF, not just the leaf blade. Take at least 70 leaves, randomly across the sampling area. Send the whole leaves to the laboratory. Begin sampling at 10 leaf stage. Monitor fortnightly until flowering peak. 1st fully expanded leaf
8 Avocado First fully expanded leaf Take the first fully expanded leaves on the latest flush. Sample at least 60 leaves through the orchard, no more than 3 per tree. Sample at about shoulder height, at up to three different points around the tree. Begin sampling at fruit set, then monthly to harvest, or sample by crop stage. Usual sampling times for sap analyses are: early fruit set when fruit size is about 25 mm when fruit is 50mm when fruit reaches mature size
9 Baby beets Take first fully expanded leaf - usually the fourth or fifth leaf out from the crown of the plant. Take at least 30 leaves, randomly across the sampling area. The petiole and midrib of the leaf is required. 1st fully expanded leaf from growing point
10 Banana Take the first fully unrolled leaf out from the crown, and chop off about 25 cm of the midrib of the leaf, from where the leaf blade begins. At least 6 leaves required - the larger the sample the more representative it is of the whole block. If the sample is too bulky the midribs can be split longitudinally, and half discarded. (i.e. 12 half midribs is a better sample than 6 entire midribs, for the same volume.) Begin sampling when suckers are 2m high, and continue fortnightly or monthly as required. 30cm section of midrib from 1st unrolled leaf
11 Basil Take first fully expanded leaf - usually the fourth leaf back from the growing point of the plant. Take at least 70 leaves, randomly across the sampling area. The part to be tested is the petiole, or leaf stalk and the midrib. Begin sampling at 5 true leaf stage. Sample weekly or fortnightly until harvest. First fully expanded leaf
12 Beans Petiole to send Take first fully expanded leaf - usually the fourth or fifth leaf back from the growing point of the plant. The trifoliate leaf is formed of three smaller leaflets - BE SURE TO TAKE WHOLE LEAF, not just the leaflets. Take at least 30 leaves, randomly across the sampling area. The part to be tested is the petiole, or leaf stalk. For ease of dispatching, the leaflets can be stripped off the leaf stalk and discarded. Strip the leaf blades only, leaving the whole petiole and the three smaller offshoots. Beans begin sampling at 5 true leaf stage. Sample weekly or fortnightly to pod fill stage.
13 Brassicas 1st fully expanded leaf Take first fully expanded leaf out from the growing point of the plant. BE SURE TO TAKE WHOLE LEAF, not just the leaf blade. Take 20 to 30 leaves, randomly across the sampling area. The part to be tested is the petiole (leaf stalk) and the midrib. For ease of dispatching, the blades can be stripped off the midrib and discarded. Begin sampling at five leaf stage, continue to heading stage, weekly or fortnightly.
14 Capsicum Take first fully expanded leaf - usually the fourth or fifth leaf back from the growing point of the plant, or out from the crown of the plant. BE SURE TO TAKE WHOLE LEAF, not just the leaf blade. Take at least 50 leaves, randomly across the sampling area. Send the whole leaves to the laboratory. First fully expanded leaf Begin sampling at early budding. Monitor weekly or fortnightly until harvest. Sampling of long harvest crops can continue through harvest.
15 Carambola It is best to sample non-fruiting terminals. Take compound leaves that are almost fully expanded on the latest flush. Sample at least 40 leaves through the orchard, no more than 3 per tree. Sample at about shoulder height, at up to three different points around the tree. Begin sampling at pre-flowering stage, monthly to harvest, or sample by crop stage as below. Usual sampling times for sap analyses are - one month pre-flowering beginning of flowering early fruit set when fruit size is about 25 mm
16 Carnations Take first fully expanded leaf down from the growing point of the plant. The leaf to sample is usually the third or fourth leaf. Sample the leaf back to the node of the stem. The part sampled is the whole leaf and midrib. Take at least 80 leaves, randomly across the sampling area. Begin sampling when first new leaves reach mature stage. Sample fortnightly or monthly. Sample this leaf
17 Carrots, Turnips & Beets Take first fully expanded leaf - usually the fourth or fifth leaf out from the crown of the plant. Take at least 30 leaves, randomly across the sampling area. Whole leaves are required for carrots, and the midrib of beet, and parsnips etc. 1st fully expanded leaf from growing point Carrots: begin sampling at 5-6 leaf stage. Sample fortnightly or every three weeks to bulking up stage.
18 Celery Take the top three nodes from the stalk from the first fully expanded leaf - usually the fourth or fifth leaf out from the crown of the plant. Take at least 30 stalks, randomly across the sampling area. Begin sampling at 5-6 leaf stage. Sample fortnightly or every three weeks to pre-harvest stage.
19 Chervil Take first fully expanded leaf - usually the fourth or fifth leaf back from the growing point of the plant. Take at least 50 leaves, randomly across the sampling area. The part to be tested is the petiole, or leaf stalk and the midrib. Begin sampling at 5 true leaf stage. Sample weekly or fortnightly until harvest.
20 Chives Send 20 whole plants after removing the roots. For larger plants cut the collar at the top end of the bulb, remove green leaves, and send the leaf blades. Most bulb crops can be sampled from the time of bulb formation to last fertigation.
21 Christmas Bush Take first fully expanded leaf - usually the third or fourth leaf back from the growing point of the plant. BE SURE TO TAKE WHOLE LEAF, not just the leaflets. Take at least leaves, randomly across the sampling area. The part to be tested is the whole leaf and petiole. 1st fully expanded leaf Sampling should commence from the new flush in spring and continue monthly during the main growing season or while vegetative growth is being produced.
22 Chrysanthemum Take first fully expanded leaf down from the growing point of the plant. The leaf to sample is usually the fourth or fifth leaf down. The part sampled is the petiole and midrib. Take at least 70 leaves, randomly across the sampling area. Begin sampling when first new leaves reach mature stage. Sample fortnightly or monthly. Take the whole compound leaf
23 Citrus Sample non-fruiting terminals only. Take leaves that are about 3/4 expanded on the latest flush. Sample at least 40 leaves through the orchard, no more than 3 per tree. Sample at about shoulder height, at up to three different points around the tree. Usual sampling times for sap analyses are one month pre-flowering beginning of flowering early fruit set when fruit size is about 25 mm Monitoring program 1st mature leaf of current flush Begin sampling at pre-flowering stage, monthly to harvest, or sample by crop stage.
24 Coffee Take the first fully expanded leaves on the latest flush. Sample at least 40 leaves through the orchard, no more than 3 per tree. Sample at about shoulder height, at up to three different points around the tree. Begin sampling at fruit set, then monthly to harvest, or sample by crop stage. First fully expanded leaf
25 Coriander Take first fully expanded leaf - usually the fourth or fifth leaf back from the growing point of the plant. Take at least 50 leaves, randomly across the sampling area. The part to be tested is the petiole, or leaf stalk and the midrib. Begin sampling at 5 true leaf stage. Sample weekly or fortnightly until harvest.
26 Corn Select the first leaf with a visible dewlap. Select the thickest stalk in each stool. Take at least leaves per sample, selected randomly through the sampling area. Avoid distorted of stunted plants Send whole leaves from young plants, but remove the leaf tips to leave 30 cm of leaf when sampling more mature plants. Sampling can begin from about 30 cm high (V5). Sample fortnightly or as required, depending on fertigation frequency. If necessary, seedlings (V1-V4) can be sampled by sending 30 whole plants.
27 Cotton Take first fully expanded leaf - usually the fourth or fifth leaf back from the growing point of the plant. BE SURE TO TAKE WHOLE LEAF, not just the leaf blade. Take at least 50 leaves, randomly across the sampling area. The part to be tested is the petiole, or leaf stalk. For ease of dispatching, the blades can be stripped off the leaf stalk and discarded. Send the petioles or leaf stalks to the Lab. Sampling times for cotton are: squaring 2-3 weeks later, late flowering 2-3 weeks later, late boll set/early boll fill 2-3 weeks later, mid-late boll fill late boll fill
28 Cucumber 1st fully expanded leaf Take first fully expanded leaf - usually the fourth or fifth leaf back from the growing point of the runner, or out from the crown of the plant. BE SURE TO TAKE WHOLE LEAF, not just the leaf blade. Take at least 30 leaves, randomly across the block. Up to 50 leaves may be required if petioles are very small. The part to be tested is the petiole, or leaf stalk. For ease of dispatching, the blades can be stripped off the leaf stalk and discarded.
29 Custard Apple It is best to sample non-fruiting terminals. Take leaves that are about 3/4 expanded on the latest flush. Sample at least 40 leaves through the orchard, no more than 3 per tree. Sample at about shoulder height, at up to three different points around the tree. Usual sampling times for are: one month pre-flowering beginning of flowering early fruit set when fruit size is about 25 mm when fruit size is about 75 mm
30 Eggplant First fully expanded leaf Take first fully expanded leaf - usually the fourth or fifth leaf back from the growing point of the plant, or out from the crown of the plant. BE SURE TO TAKE WHOLE LEAF, not just the leaf blade. Take at least 30 leaves, randomly across the block. The part to be tested is the petiole, or leaf stalk and the midrib of the leaf. Begin sampling at budding, continue weekly or fortnightly until harvest. May continue sampling during harvest.
31 Fennel Take the top 20cm of the plant. Take at least 30 tops, randomly across the sampling area. Begin sampling at budding; and monitor fortnightly to mid-harvest.
32 Gerberas Take first fully expanded leaf out from the crown of the plant. This will be the leaf that is almost fully expanded and starting to change colour to a darker green. The part sampled is the petiole and midrib. Take at least 30 leaves, randomly across the sampling area. Begin sampling when first new leaves reach mature stage. Sample fortnightly or monthly. Sample this leaf
33 Grapes Take first fully expanded leaf - usually the fourth or fifth leaf back from the growing point of the runner. BE SURE TO TAKE WHOLE PETIOLE. Take at least 60 leaves, randomly across the block and increase number if petioles are small. The part to be tested is the petiole, or leaf stalk. For ease of dispatching, the blades can be stripped off the leaves of grapes and discarded. Begin sampling when canes are 30 cm long. Continue weekly or fortnightly to harvest. Strategic sampling times are 30 cm canes, early flowering, early fruit set, fruit fill, pre-harvest, and postharvest.
34 Industrial Hemp Select the youngest fully expanded leaf back from the growing point BE SURE TO TAKE WHOLE LEAF, not just the leaflets. Take at least 50 leaves, randomly across the sampling area. The part to be tested is the petiole, or leaf stalk. For ease of dispatching, the leaflets can be stripped off the leaf stalk and discarded. Begin sampling at 30cm in height. Sample fortnightly to flowering stage. 1st fully expanded leaf
35 Kangaroo Paw Take first fully expanded leaf - usually the second or third leaf out from the growing point of the plant, or out from the crown of the plant. Take at least 50 leaves, randomly across the sampling area. Send the entire leaves to the laboratory. Begin sampling when first able to sample appropriate part. Monitor monthly until flowering. 1st fully expanded leaf
36 Kiwi Fruit Take first fully expanded leaf - usually the fourth or fifth leaf back from the growing point of the runner. BE SURE TO TAKE WHOLE LEAF, not just the leaf blade. Take at least 50 leaves, randomly across the block. The part to be tested is the petiole, or leaf stalk and midrib. Begin sampling when vines reach 1 m and sample monthly until midharvest.
37 Lemon Grass Take the first fully expanded leaf. The part to be tested is the midrib of the leaves. Send leaves from across the sampling area.
38 Lettuce & Cabbage Take wrapper leaf, close to head maturity. Take at least 15 leaves, randomly across the block. The part to be tested is the midrib of the leaf. For ease of dispatching, the blades can be stripped off the midrib and discarded. Begin sampling at 3-4 weeks and sample weekly or fortnightly until two weeks before harvest.
39 Loquats Sample non-fruiting terminals only. Take leaves that are almost fully expanded on the latest flush. Sample at least 50 leaves through the orchard, no more than 3 per tree. Sample at about shoulder height, at up to three different points around the tree. Begin sampling at early fruit set, then monthly to harvest, or sample by crop stage. Usual sampling times for sap analyses are - one month pre-flowering beginning of flowering early fruit set at fruit fill stage
40 Lucerne Take the top 20cm of the plant. Take at least 30 tops, randomly across the sampling area. Begin sampling at budding; and monitor fortnightly to mid-harvest.
41 Lychee Sample non-fruiting terminals only. Take leaves that are almost fully expanded on the latest flush. Sample at least 200 leaves through the orchard, no more than 3 per tree. Sample at about shoulder height, at up to three different points around the tree. Begin sampling at pre-flowering stage, monthly to harvest, or sample by crop stage as below. Usual sampling times for sap analyses are - one month pre-flowering beginning of flowering early fruit set when fruit size is about 25 mm
42 Macadamia First fully expanded leaf Take the first fully expanded leaves on the latest flush. Sample at least 200 leaves through the orchard, no more than 3 per tree. Sample at about shoulder height, at up to three different points around the tree. Begin sampling at fruit set, then monthly to harvest, or sample by crop stage. Usual sampling times for sap analyses are - early fruit set when fruit size is about 25 mm when fruit is 50mm when fruit reaches mature size
43 Mango 1st fully expanded leaf Sample non-fruiting terminals only. Take leaves that are about 3/4 expanded on the latest flush. Sample at least 70 leaves through the orchard, no more than 3 per tree. Sample at about shoulder height, at up to three different points around the tree. Begin sampling at early fruit set, then monthly to harvest, or sample by crop stage. Usual sampling times for sap analyses are - one month pre-flowering beginning of flowering early fruit set when fruit size is about 25 mm at fruit fill stage
44 Mint Take the top 20cm of the plant. Take at least 30 tops, randomly across the sampling area. Begin sampling at budding; and monitor fortnightly to midharvest.
45 Olives Sample non-fruiting terminals only. Take leaves that are fully expanded on the latest flush. Sample at least 70 leaves through the orchard, no more than 3 per tree. Sample at about shoulder height, at up to three different points around the tree. Monitoring program Begin sampling when first leaves of new flush reach maturity. Sample fortnightly or monthly to peak harvest. 1st fully expanded leaf
46 Onions, Garlic & Leek For spring onions and young onions, send 20 whole plants after removing the leaf tips and roots. Be sure to include the collar at the base of the leaf, as this is the part to be tested. For larger plants cut the collar at the top end of the bulb, remove green leaves, and send the collars collars is enough. Most bulb crops can be sampled from the time of bulb formation to last fertigation.
47 Parsley Take first fully expanded leaf - usually the fourth or fifth leaf back from the growing point of the plant. Take at least 50 leaves, randomly across the sampling area. The part to be tested is the petiole, or leaf stalk and the midrib. Begin sampling at 5 true leaf stage. Sample weekly or fortnightly until harvest.
48 Passionfruit Take first fully expanded leaf - usually the fourth or fifth leaf back from the growing point of the runner. BE SURE TO TAKE WHOLE LEAF, not just the leaf blade. Take at least 70 leaves, randomly across the block. The part to be tested is the petiole, or leaf stalk and midrib. Begin sampling when vines reach 1 m and sample monthly until midharvest. 1st fully expanded leaf
49 Paw Paw Take the youngest fully expanded leaf out from the crown, and remove the petiole. At least 10 petioles required. Petioles should be approximately 25 cm long. Leaf blades can be removed before dispatching sample. Sample from budding, monthly to harvest. Petiole of first fully expanded leaf
50 Peanut Take the first fully expanded leaf from the growing tip of the plant. The trifoliate leaf is formed of three smaller leaflets BE SURE TO TAKE THE WHOLE LEAF AND PETIOLE, not just the leaflets. Take at least 50 leaves randomly across the sampling area. The part to be tested is the petiole or leaf stalk. For ease of dispatching the leaflets can be stripped off and discarded. Strip the leaf blades only leaving the petiole and the three smaller offshoots. Begin sampling when the first new leaves reach the mature stage. Sample every 2-4 weeks until the plants start to shut down. Important times are pre-flowering, pegging and nut sizing.
51 Peas & Snow Peas Take the top 20cm of the plant. Take at least 30 tops, randomly across the sampling area. Begin sampling at budding; and monitor fortnightly to midharvest.
52 Pistachio Nuts Sample non-fruiting terminals only. Take leaves that are fully expanded on the latest flush. Sample at least 50 leaves through the orchard, no more than 3 per tree. Sample at about shoulder height, at up to three different points around the tree. Monitoring program Begin sampling when first leaves of new flush reach maturity. Sample fortnightly or monthly to peak harvest. 1st fully expanded leaf
53 Potato First fully expanded leaf Take first fully expanded leaf - usually the fourth or fifth leaf back from the growing point of the plant. The compound leaf is formed of several/ many smaller leaflets - BE SURE TO TAKE WHOLE LEAF, not just the leaflets. Take at least 30 leaves, randomly across the sampling area. The part to be tested is the petiole, or leaf stalk. In potatoes, this should be from 15 to 30 cm long, depending on the stage of the crop. For ease of dispatching, the leaflets can be stripped off the leaf stalk and discarded. Begin sampling at 4-5 leaf stage. Sample fortnightly to bulking up stage.
54 Rhubarb Take the stalk from the first fully expanded leaf - usually the fourth or fifth leaf out from the crown of the plant. Take at least 30 stalks, randomly across the sampling area. Begin sampling at 5-6 leaf stage. Sample fortnightly or every three weeks to "preharvest" stage.
55 Rocket Take first fully expanded leaf - usually the fourth or fifth leaf back from the growing point of the plant. Take at least 50 leaves, randomly across the sampling area. The part to be tested is the petiole, or leaf stalk and the midrib. Begin sampling at 5 true leaf stage. Sample weekly or fortnightly until harvest.
56 Rose Take first fully expanded leaf from the growing point of the plant. The compound leaf is formed of five smaller leaflets - BE SURE TO TAKE WHOLE LEAF, not just the leaflets. Take at least 50 leaves, randomly across the sampling area. Begin sampling when first new leaves reach mature stage. Sample fortnightly or monthly. Take the whole compound leaf
57 Rosemary Take the top cm of the new growth. Take at least 20 to 30 tops, randomly across the sampling area.
58 1st fully expanded leaf Sage Take first fully expanded leaf - usually the fourth or fifth leaf back from the growing point of the plant. BE SURE TO TAKE WHOLE LEAF, not just the leaf blade. Take at least 40 leaves, randomly across the block. The part to be tested is the petiole, or leaf stalk. For ease of dispatching, the blades can be stripped off the leaf stalk and discarded.. Begin when the plant begins to start significant vertical growth and continue every 2-4 weeks until harvest.
59 Stone & Pome Fruit Sample non-fruiting terminals only. Take leaves that are fully expanded on the latest flush. Sample at least 30 leaves through the orchard, no more than 3 per tree. Sample at about shoulder height, at up to three different points around the tree. Monitoring program Begin sampling when first leaves of new flush reach maturity. Sample fortnightly or monthly to peak harvest. 1st fully expanded leaf
60 Strawberry Take first fully expanded leaf out from the crown of the plant. The trifoliate leaf is formed of three smaller leaflets - BE SURE TO TAKE WHOLE LEAF AND PETIOLE, not just the leaflets. Take at least 70 leaves, randomly across the sampling area. Send petiole The part to be tested is the petiole, or leaf stalk. For ease of dispatching, the leaflets can be stripped off the stalk and discarded. Strip the leaf blades only, leaving the whole petiole and the three smaller offshoots. Begin sampling when first new leaves reach mature stage. Sample fortnightly until the end of the main harvest period.
61 Sugarcane, Sorghum & Maize Select the first leaf with a visible dewlap. Select the thickest stalk in each stool. Take at least leaves per sample, selected randomly through the sampling area. Avoid distorted or stunted plants Send whole leaves from young plants, but remove the leaf tips to leave 30 cm of leaf when sampling more mature plants. Sampling can begin from about 30 cm high, in conjunction with Quick Soil test. Sample fortnightly or as required, depending on fertigation frequency. If necessary, seedlings can be sampled by sending 30 whole plants.
62 Sweet Potato Take first fully expanded leaf - usually the fourth or fifth leaf back from the growing point of the plant, or out from the crown of the plant. BE SURE TO TAKE WHOLE LEAF, not just the leaf blade. Take at least 50 leaves, randomly across the block. The part to be tested is the petiole, or leaf stalk. For ease of dispatching, the blades can be stripped off the leaf stalk and discarded. 1st fully expanded leaf Begin when runners are 50 cm long, continue fortnightly to bulking up.
63 Tarragon Take the top 20cm of the plant. Take at least 30 tops, randomly across the sampling area. Begin sampling at budding; and monitor fortnightly to midharvest.
64 Timber Trees 1st fully expanded leaf Take leaves that are about 3/4 expanded on the latest flush. Sample at least 80 leaves through the plantation, no more than 3 per tree. Sample at about shoulder height if possible, (or from limbs that have been recently removed) at up to three different points around the tree. Usual sampling times for sap analyses are - as required during main growing period of the year main sampling period is during the first 2-4 years of tree establishment
65 Tomato 1st fully expanded leaf Take first fully expanded leaf - usually the fourth or fifth leaf back from the growing point of the plant. The compound leaf is formed of several/many smaller leaflets - BE SURE TO TAKE WHOLE LEAF, not just the leaflets. Take at least 30 leaves, randomly across the block. The part to be tested is the petiole, or leaf stalk. In tomatoes, this should be from 15 to 30 cm long, depending on the stage of the crop. For ease of dispatching, the leaflets should be stripped off the leaf stalk and discarded. Tomatoes begin sampling at first flowering, continue weekly or fortnightly until harvest. May continue sampling trellised crops after harvest has begun.
66 Thyme Take the top 5-10 cm of the new growth. Take at least 40 to 50 tops, randomly across the sampling area.
67 Vine Cucurbits Watermelon, rockmelon, honeydew & pumpkin. First fully expanded leaf - usually the fourth or fifth leaf back from the growing point of the runner, or out from the crown of the plant. BE SURE TO TAKE WHOLE LEAF, not just the leaf blade. Take at least 30 leaves, randomly across the block. Up to 50 leaves may be required if petioles are very small. 1st fully expanded leaf The part to be tested is the petiole, or leaf stalk. For ease of dispatching, the blades can be stripped off the leaf stalk and discarded.
68 Watercress Take the top 20cm of the plant. Take at least 30 tops, randomly across the sampling area. Begin sampling at budding; and monitor fortnightly to midharvest.
69 Wheat, Barley & Oats (early stage) Sample the whole above ground part of the plant. The whole plant is sampled up until tillering begins. Send plants per sample, selected randomly through the sampling area. Avoid distorted of stunted plants in the sampling area. Usually a single sample is sufficient from this growth stage.
70 Wheat, Barley & Oats (tillering) Sample the first fully expanded leaf and sheath with a visible dewlap. This part is sampled up until the flag leaf emerges. Send leaves per sample, selected randomly through the sampling area. Avoid distorted of stunted plants in the sampling area. Usually a single sample is sufficient from this growth stage. Leaf with visible dewlap
71 Wheat, Barley & Oats (flag leaf stage) Sample the flag leaf and sheath down to the last node. The flag leaf will be the final leaf that emerges before the grain head emerges. Send leaves per sample, selected randomly through the sampling area. Avoid distorted of stunted plants in the sampling area. Usually a single sample is sufficient from this growth stage. Flag Leaf, final leaf before grain head
72 Zucchini & Squash Take first fully expanded leaf - usually the fourth or fifth leaf back from the growing point of the runner, or out from the crown of the plant. BE SURE TO TAKE WHOLE LEAF, not just the leaf blade. Take at least 30 leaves, randomly across the block. Up to 50 leaves may be required if petioles are very small. No more than 20 leaves may be required for zucchinis. 1st fully expanded leaf The part to be tested is the petiole, or leaf stalk. For ease of dispatching, the blades can be stripped off the leaf stalk and discarded.
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