A Preliminary Study on the Extraction Techology of Oil-tea Camellia Seed Oil by Aqueous Enzymatic Method
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1 http / /xuebao. jxau. edu. cn Acta Agriculturae Universitatis Jiangxiensis E - mail ndxb7775@ sina. com * Alcalase 2. 4 L % Alcalase 2. 4 L % Alcalase 2. 4 L TS A A Preliminary Study on the Extraction Techology of Oil-tea Camellia Seed Oil by Aqueous Enzymatic Method SUN Hong FEI Xue-qian * FANG Xue-zhi Research Institute of Subtropical Forestry CAF Fuyang China Abstract An aqueous enzymatic extraction technology was adopted to extract oil from Oil - tea camellia seed. The influences of different enzymes and enzyme complex formulas on oil yield were investigated. The result showed that Alcalase 2. 4 L was the best single enzyme with the highest oil yield of %. The best enzyme complex formula was composed by pectinase cellulose and Alcalase 2. 4 L which was gained by multilevel optimization procedure. The oil yield efficiency could reach % when this enzyme complex formula was used in sequence. By comprehensive consideration Alcalase 2. 4 L is the best hydrolase. Key words oil-tea camellia seed oil aqueous enzymatic method enzymatic selection oil yield BADB1B E - mail sunh206@ 163. com * E - mail fxq6565@ 163. com
2 % % sigma C2730 Trichoderma reesei ATCC ~ 60 ph 4. 5 ~ 6. 0 sigma H2125 Aspergillus niger 40 ph 4. 5 sigma P2736 Aspergillus niger 50 ph 8. 0 sigma Bacillus subtilis 25 ph 6. 9 Alcalase 2. 4 L Novozymes Alcalase 2. 4 L Bacillus licheniformis 50 ~ 60 ph 8 ~ 9 Buchi B Thermo Fisher ph g 50 ml 25 ml min ph min r /min 30 min GB /T = - / 100% / 1 5 g 5 50 ml 2% h r /min 30 min 2 2% h 1 Tab. 1 1 Enzymatic hydrolysis conditions of simultaneous enzyme supplementation /% ph / /h Emzyme combination Enzyme dosage Reaction ph Reaction temperature Reaction time + Alcalase Cellulose + Alcalase Alcalase Hemicellulase + Alcalase Alcalase Pectinase + Alcalase Alcalase Amylase + Alcalase % h
3 Alcalase Alcalase % % Alcalase % % % 5. 97% Fig. 1 The influences of different enzyme type on oil yield Alcalase Alcalase 2 + Alcalase Alcalase 1 Cellulase + Alcalase 2 Pectinase + Alcalase 3 Hemicellulase + Alcalase 4 Amylase + Alcalase. 2 Fig. 2 The influences of different two - enzymes complex formulas on oil yield Alcalase Alcalase % % 1. 73% Alcalase 1 + Alcalase 2 /Alcalase 3 Alcalase / 4 + Alcalase 5 /Alcalase 6 Alcalase / Alcalase + / 1 Cellulase + Alcalase 2 Cellulase /Alcalase 3 Alcalase /Cellulase 4 Pectinase + Alcalase 5 Pectinase /Alcalase 6 Alcalase /Pectinase. + means simultaneous enzyme supplementation / means different adding sequence. Alcalase 3 Fig. 3 The influences of different adding sequence on oil yield
4 /Alcalase 2 3 /Alcalase 4 1 / /Alcalase 2 / /Alcalase 3 / /Alcalase 4 / /Alcalase 5 / /Alcalase 1 Cellulase/Pectinase/Alcalase 1 Cellulase /Alcalase 2 Pectinase /Alcalase 2 Cellulase/Hemicellulase/Alcalase 3 Cellulase/Amylase/Alcalase 3 /Alcalase 4 Amylase + Alcalase. 4 Pectinase/Hemicellulase/Alcalase 5 Pectinase /Amylase /Alcalase. 4 5 Fig. 4 The influences of different enzymes Fig. 5 The influences of different tri enzymes complex formulas on oil yield complex formulas on oil yield Alcalase % Alcalase Alcalase % 0. 31% Alcalase 2 ph Alcalase Alcalase Alcalase Alcalase % % 0. 39% Alcalase Alcalase / /Alcalase 2 / /Alcalase 3 Alcalase + /Alcalase Alcalase 5 Alcalase / / 6 Alcalase / / % 0. 44% 1 Cellulase /Pectinase /Alcalase 2 Pectinase /Cellulase /Alcalase 3 91% Cellulase + Pectinase /Alcalase 4 Cellulase + Pectinase + Alcalase 5 Alcalase /Cellulase /Pectinase 6 Alcalase /Pectinase /Cellulase. 6 Alcalase Fig. 6 The influences of different adding sequence on oil yield
5 Alcalase % Alcalase 6 Alcalase % % % % 0. 44% 0. 92% Alcalase 2. 4 L % Alcalase2. 4 L 3 1 Alcalase % 2 Alcalase 2. 4 L % Alcalase 2. 4 L 3 1. J J Zhang S B Wang Z Xu S Y. Optimization of the aqueous enzymatic extraction of rapeseed oil and protein hydrolysates J. Journal of the American Oil Chemists Society Moreau R A Johnston D B Powell M J et al. A comparison of commercial enzymes for the aqueous enzymatic extraction of corn oil from corn germ J. Journal of the American Oil Chemists Society J J J J Dominguez H Nunez M J Lema J M. Enzymatic pretreatment to enhance oil extraction from fruits and oilseeds A review J. Food Chemistry Rosenthal A Pyle D L Niranjan K. Aqueous and enzymatic processes for edible oil extraction J. Enzyme and Microbial Technology GB /T S Huang A. Structure of plant seed oil bodies J. Current Opinion in Structural Biology Yoon SH Kim IH Kwon TW. Effects of enzyme treatments and ultrasonification on extraction yields of lipids and protein from soybean by aqueous process J. Korean J J J
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