what news? an essential step
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- Shana Glenn
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1 what news? an essential step - Identify ways to optimize the facility s efficiency - Evaluate common needs - Discuss ways to fund new equipments & scientific contributions
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4 Dr. Wehrle-Haller Pr. Scorrano Pr. Sizonenko Pr. Schrenzel Pr. Demaurex Pr. Martinvalet Pr. Imhof Pr. Krause Pr. Jaconi Half of the equipment was acquired with the help of a scientific contribution as shown in blue + Laser Capture LMD + Olympus VS120 (Slide reader) Pre. Gotta Pre. Wohlwend Collaboration /partnership
5 2012 New mirrors New camera New microscope New Yokogawa head Definite focus Software updated New microscope New TIRF arm Perfect focus Software updated 2014 New Widefield system + DIC optics 2012 New CLSM 2014 New CLSM (soon!) Two-thirds of the machine park was upgraded or renewed these last 2 years as shown in red 2011 New CLSM New detectors GaAsp Software updated New system New optics + Laser Capture LMD + Olympus VS120 (Slide reader) New camera, new module for automation (software) 2012 New system
6 kfrs 160 kfrs 100 kfrs 36 kfrs Total (last 2 years): Frs kfrs kfrs 38 kfrs 80 kfrs + Laser Capture LMD 30 kfrs Olympus VS120 (Slide reader) 150 kfrs Running costs: Frs () Frs (2012)
7 High (>1500 hours) Medium ( hours) Low (<500 hours) + Laser Capture LMD + Olympus VS120 (Slide reader)
8 + Laser Capture LMD + Olympus VS120 (Slide reader)
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10 Total Frs (acquisition) and then Frs /year (updates, 10 %)
11 background focus Evanescent Wave Microscopy (TIRFM*) Confocal Laser Scanning Microscopy (CLSM) Fluorescence Widefield Microscopy Nipkow Spinning Disk Confocal Microscopy Two-Photon Microscopy + Laser Dissection Microscope + automated systems (Mirax/ slide scanner, ImageXpress/plate reader,.)
12 Spatial and temporal resolution of biological imaging techniques
13 Wavelength Lens PSF
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15 Structured Illumination Microscopy Stimulated Emission Depletion Photoactivated Localization Microscopy Stochastic Optical Reconstruction Microscopy
16 Schermelleh L et al. JCB 2010;190:
17 CLSM SIM Schermelleh et al. Science (2008)
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19 CLSM PALM (from:
20 The acquisition of the future SR-technique will be determined by the demands of the future R equip application (in 2014)!
21 -1) Identify ways to optimize the facility s efficiency -2) Evaluate common needs -3) Discuss ways to fund new equipments & scientific contributions
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