Principal Component Analysis and Cluster Analysis for Main Morphological Characteristics of Cauliflower Inbred Lines

Transcription

1 Journal of Plant Genetic Resources / % Ⅰ Ⅱ Ⅲ 3 Principal Component Analysis and Cluster Analysis for Main Morphological Characteristics of Cauliflower Inbred Lines ZHU Shi-yang ZHANG Xiao-ling LIU Qing TANG Zheng LUO Tian-kuan JING Zan-ge Key Laboratory of Crop Breeding in South Zhejiang / Wenzhou Academy of Agricultural Science Wenzhou Abstract Classification of cauliflower resource was useful for heterosis and hybridization selection. 54 cauliflower inbred lines were analyzed with statistical methods of cluster analysis and the principal component analysis PCA. The PCA results showed that the six eigenvalues of cumulative variance proportion % were selected for evaluation of inbred line of cauliflower Days to curd appearing days to curd harvesting leaf length leaf width plant height lengthways diameter of curd transverse diameter of curd weight curd shape tight degree leaf color wax powder leaf number inside days to flowering and plant extent were morphological characteristics for parents screening. By cluster analysis under the results of PCA 54 cauliflower inbreed lines were classified into 3 clusters. Characters of clusterⅠwere early maturity little plant extent narrow leaf with less wax moderate weight and tight degree of curd Cluster Ⅱ had the characters of middle maturity moderate plant extent sage green leaves with thick wax and weighty curd with tight semicircular Cluster Ⅲ was type of late maturity high stalk large plant extent more length and width leaves with moderate wax and oblate curd. Three clusters had obvious feature so that it would be helpful for parent materials selection in breeding. Key words Cauliflower Inbred line Morphological characteristic Principal component analysis Cluster analysis Brassica oleracea L. Var. botrytis L Y C22094 N N N zhushiyang2000@ 163. com

2 AFLP 8 RAPD cm 50cm Table 1 Code and origins of 54 cauliflower inbred lines 1. 3 Code of Code of Code of Origin Origin Origin inbred line inbred line inbred line AYXF-80 YK-F 1-80 FZTX-60 AYXF-90 AJ-90 FZTD-80 BMWZ-60 JSTD-50 ZHIHUA DHMZ-50 JSTD-80 YX-60 L-45 JZ-80 SH-80 LFTD-60 LM-80 TDXG-80 RG-F 1-80 LY-70 TDXG-100 RG-F SN-65 SLTD-60 RG-F TB-80 CJBY-50 RXTD-80 WX-60 YDSL-60 RXTD-90 WX-80 XS-45 RXTD-120 WX-90 YG-40 RXTZ-50 YDXG-50 U 1 TZ-45 YDXL-75 U 2 XTS-60 YDZN-50 U 3 YDJG-45 YN-70 U 4 YDJG-50 YN-80 U 5 YDJG-55 ZH-80 U Excel DPS % % % % ~ % l %

3 Table 2 Differences in numerical characteristics of 54 cauliflower inbred lines Characteristic Max Min Mean s % CV d Days to curd appearing d Days to curd harvesting d Days to flowering cm Leaf length cm Leaf width Leaf number outside Leaf number inside cm Plant extent cm Lengthways diameter of curd cm Transverse diameter of curd cm Plant height kg Cur weight Table 3 20 Principal components of 20 morphological characteristics of cauliflower Characteristic Principal components d Days to curd appearing d Days to curd harvesting d Days to flowering cm Leaf length cm Leaf width Leaf number outside Leaf number inside cm Plant extent cm Lengthways diameter of curd cm Transverse diameter of curd cm Plant height kg Cur weight Curd shape Curd color Tight degree of curd Hair Leaf color Leaf crimpling Wax powder Flower color Eigenvalue % Contributive percentage % Cumulative contributive percentage

4 Table 4 Indies and eigenvectors of principal components of morphological characteristics of cauliflower Principal components Index Eigenvector % % % Fig. 1 Dendrogram reflecting genetic relationships of 54 cauliflower inbred lines 5 4 ~ 6 Table 5 Average of morphological characteristics in different % ~ % clusters DPS Characteristic ClusterⅠ ClusterⅡ ClusterⅢ d d d cm cm cm cm

5 1 81 Characteristic ClusterⅠ ClusterⅡ ClusterⅢ cm cm kg The number in parentheses was the average evaluating of curd shape curd color tight degree of curd hair leaf color leaf crimpling wax powder and flower color % % Ⅰ 14 AYXF RXTZ-50 RXTD-80 BMWZ-60 DHMZ-50 TZ % YDJG-55 YDZN-50 U 2 CJBY-50 XS-45 SN-65 1 TDXG-80 YG d Ⅱ 22 L-45 WX YDJG-50 YN-70 JSTD-50 FZTX-60 U 3 SLTD- 60 YDXG-50 LFTD-60 RG-F 1-80 YK-F 1-80 U 1 JSTD-80 YDXL-75 JZ-80 LY-70 LM-80 YN-80 TB-80 FZTD-80 YDSL d kg Ⅲ 18 AYXF-90 AJ- 90 WX-90 RG-F RXTD-120 RXTD-90 WX- 80 YX-60 SH-80 U 4 U 6 XTS-60 YDJG-45 ZH- Ⅰ 80 ZHIHUA TDXG-100 U 5 RG-F Ⅱ 95. 1d Ⅱ Ⅲ 54

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