How we turn salmon, shrimp, and other marine animals into vegetarians. Christoph Kobler June 21, 2016, Essen, Germany

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1 How we turn salmon, shrimp, and other marine animals into vegetarians Christoph Kobler June 21, 2016, Essen, Germany

2 Aquaculture is the growth driver in global fish production Development in wild-caught vs. farmed fish Millions of metric tons 75% 25% 50% 50% 25% 75% ~ 90 million metric tons per year Aquaculture Wild-capture fisheries Fish are among the most important sources of protein in human diets The world s oceans are overfished and overexploited Aquaculture already accounts for 50% of all fish, crustaceans and other marine species Page 2

3 Vision: quality and sustainability in aquaculture Our vision for sustainable aquaculture: Healthy animals Highly efficient feeding Minimizing the use of fishmeal and fish oil in feeds Reducing water pollution Page 3

4 Transfer the salmon success story to shrimp Traditional salmon diet (2007) Current salmon diet (2014) Future salmon diet (medium term) Fishmeal Other Fishmeal Other 35% 40% 15% 10% 74% Fishmeal Fish oil Other 84% 5% 5% 5% Fish oil Amino acids > 1% Omega-3 oil from algae > 5% 25% Fish oil Amino acids < 1% The salmon industry will finally become a net fish producer: fish in : fish out < 1 (today: net fish consumer!) Page 4

5 Eating habits and digestive systems of crustaceans differ from those of fish Primitive digestive system Slow feed intake Fast response of supplemented free amino acids Feed pellet is under water for a long time Fast excretion Eutrophication of water Amino acid synchronicity problem Poor growth Leaching of water soluble nutrients Page 5

6 Requirements of a suitable methionine product for shrimps and other crustaceans Poor water solubility Leaching protection High hydrolytic stability Small particle size New methionine derivative High active ingredient content Slow release in the gut (retarder) Stable in pelletization/ extrusion process No interaction with feed ingredients Page 6

7 The search for the right molecule took several years Development and screening of promising methionine derivatives Chemical syntheses Application tests Decision Synthesis development Pelletization tests 35 different methionine derivatives In vitro digestion experiments Hydrolytic stability tests Met-Met Solubility and release tests Page 7

8 The solution: a dipeptide DL-Methionyl-DL-Methionine The dipeptide of DL-methionine DL-Met molecule 1 O O O S N H S NH 3 DL-Met molecule 2 Low solubility of the dipeptide in water means very little leaching from the feed High stability during feed processing, in the feed pellet and in water Slow release and synchronous availability of methionine via enzymatic cleavage Page 8

9 Outstanding bioefficacy of AQUAVI Met-Met in shrimp feeding trials Example: Dose response trial with whiteleg shrimp (Litopenaeus vannamei) 9, % Specific specific growth growth rate rate, (% %/d per day) 8, , , ,0 7.0 Basal diet DL-Methionine AQUAVI Met-Met Met-Met 6, , , ,20 0, , ,50 0, ( ) Supplemented supplemented methionine product, source % of (% dietof diet) Relative bioefficacy (%) % DL-Met AQUAVI Met-Met Page 9

10 AMINOShrimp Amino acid recommendations for optimized shrimp nutrition The new innovative software gives the customer the opportunity to calculate species specific amino acid recommendations for different shrimp adjusted to important parameters like initial weight, feeding rate, feeding frequency, growth rate and production system. Page 10

11 Optimized particle size distribution of AQUAVI Met-Met ensures high homogeneity in feed mixtures Light microscopic images (100x) Competing product 1 Competing product 2 90% of AQUAVI Met-Met particles are smaller than 300 μm Optimal blending with other feed components Ensures that each individual feed pellet will have the same Met-Met dipeptide content Page 11

12 Goal achieved Minimized leaching from feed pellet Scientifically proven outstanding growth performance Excellent homogeneity and distribution in the feed mixing process Competitive manufacturing costs Successful development of innovative low fishmeal diet concepts Patented application and process Page 12

13 Production facility in Antwerp Facility is fully integrated in an existing Evonik methionine plant to form a Verbund Startup capacity: 3,000 metric tons of AQUAVI Met-Met per year First industrial-scale dipeptide synthesis in purely aqueous solutions without the use of organic solvents Page 13

14 Principle markets: Asia and Central America 2% Mexico 2% Bangladesh 5% Thailand 8% ROW 5% Ecuador 6% (400) India 8% (500) Vietnam 2014 global 6.60 million metric tons 9% (600) Indonesia 5% (3,700) China Target markets in Asia: ~ 90% of the global shrimp and crustacean market Source: FAO 2014 Page 14

15 Outlook Research on additional applications for AQUAVI Met-Met in aquatic and land animals Collaboration with DSM Nutritional Products Ltd. to develop algae-based omega-3 fatty-acid products Medium-term goal: vegetarian shrimp feed Page 15

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