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1 This article was downloaded by:[steele, Brooke] On: 6 F ebruary 2007 Access Details: [subscription number ] Publisher: Taylor & Francis Informa Ltd Registered in England and Wales Registered Number: Registered office: Mortimer House, Mortimer Street, London W1T 3JH, UK C omputer Methods in Biomechanics and Biomedical E ngine ering Publication details, including instructions for authors and subscription information: Fractal network model for simulating abdominal and lower extremity blood flow during resting and exercise conditions To link to this article: D OI: / URL: Full terms and conditions of use: This article maybe used for research, teaching and private study purposes. Any substantial or systematic reproduction, re-distribution, re-selling, loan or sub-licensing, systematic supply or distribution in any form to anyone is expressly forbidden. The publisher does not give any warranty express or implied or make any representation that the contents will be complete or accurate or up to date. The accuracy of any instructions, formulae and drug doses should be independently verified with primary sources. The publisher shall not be liable for any loss, actions, claims, proceedings, demand or costs or damages whatsoever or howsoever caused arising directly or indirectly in connection with or arising out of the use of this material. Taylor and Francis 2007

2 Computer Methods in Biomechanics and Biomedical Engineering, Vol. 10, No. 1, February 2007, Fractal network model for simulating abdominal and lower extremity blood flow during resting and exercise conditions BROOKE N. STEELE *, METTE S. OLUFSEN and CHARLES A. TAYLOR{k Joint Department of Biomedical Engineering, NC State University & UNC-Chapel Hill, Campus Box 7115, Raleigh, NC , USA Department of Mathematics, NC State University, Campus Box 8205, Raleigh, NC , USA {Departments of Mechanical Engineering, Bioengineering, and Surgery, James H. Clark Center, Room E350B, 318 Campus Drive, Stanford, CA , USA (Received 6 August 2006; in final form 26 September 2006) We present a one-dimensional (1D) fluid dynamic model that can predict blood flow and blood pressure during exercise using data collected at rest. To facilitate accurate prediction of blood flow, we developed an impedance boundary condition using morphologically derived structured trees. Our model was validated by computing blood flow through a model of large arteries extending from the thoracic aorta to the profunda arteries. The computed flow was compared against measured flow in the infrarenal (IR) aorta at rest and during exercise. Phase contrast-magnetic resonance imaging (PC-MRI) data was collected from 11 healthy volunteers at rest and during steady exercise. For each subject, an allometrically-scaled geometry of the large vessels was created. This geometry extends from the thoracic aorta to the femoral arteries and includes the celiac, superior mesenteric, renal, inferior mesenteric, internal iliac and profunda arteries. During rest, flow was simulated using measured supraceliac (SC) flow at the inlet and a uniform set of impedance boundary conditions at the 11 outlets. To simulate exercise, boundary conditions were modified. Inflow data collected during steady exercise was specified at the inlet and the outlet boundaries were adjusted as follows. The geometry of the structured trees used to compute impedance was scaled to simulate the effective change in the crosssectional area of resistance vessels and capillaries due to exercise. The resulting computed flow through the IR aorta was compared to measured flow. This method produces good results with a mean difference between paired data to be 1.1 ^ 7 cm 3 s 21 at rest and 4.0 ^ 15 cm 3 s 21 at exercise. While future work will improve on these results, this method provides groundwork with which to predict the flow distributions in a network due to physiologic regulation. Keywords: One-dimensional model; Arterial blood flow; Fractal; Structured tree; Impedance; Exercise 1. Introduction Numerous models have been used to describe the dynamics of blood flow and blood pressure in the cardiovascular system. These models include simple Windkessel models (Pater and van den Berg 1964; Westerhof et al. 1969; Noordergraaf 1978), non-linear one-dimensional (1D) models (Stergiopulos et al. 1992; Olufsen et al. 2000; Wan et al. 2002) and complex three-dimensional (3D) models (Taylor et al. 1996; Cebral et al. 2003). Each class of models is suited to answer a particular type of blood flow question. For example, the Windkessel can be used to describe the overall dynamics of blood flow in the systemic circulation (Olufsen et al. 2000; Olufsen and Nadim 2004) while spatial models (1D, 2D and 3D models) can describe blood flow and blood pressure through a given geometry. Spatial models span a limited region of interest. The remainder of the circulatory system is represented with a set of boundary conditions that are developed to approximate blood flow and blood pressure outside the modelled domain. *Corresponding author. Tel: þ Fax: þ Tel: þ Fax: þ ktel: þ Fax: þ Computer Methods in Biomechanics and Biomedical Engineering ISSN print/issn online q 2007 Taylor & Francis DOI: /

3 40 B. N. Steele et al. To describe boundary conditions for spatial models, researchers often prescribe blood flow or pressure profiles (Taylor et al. 1999a). Although this approach is simple, specifying flow or pressure will influence the fluid dynamics inside the model domain and is only appropriate when profiles and distribution between outlets is known. Often complete boundary profile information is not available, so constant relationships between pressure and flow are used (Wan et al. 2002). Resistance boundary conditions provide a convenient method to specify a boundary relationship without prescribing a pressure or flow waveform. However, pure resistance boundary conditions cannot account for non-proportional variations between pressures and flow as observed in compliant vessels. An alternative to the constant resistance boundary condition is the impedance boundary condition, which is the frequency analogue to resistance. Impedance has long been recognized as an important tool for evaluating the reflections and damping of flow and pressure waves (Taylor 1966; Brown 1996; Nichols and O Rourke 2005). Impedance boundary conditions are often implemented using simple three-element Windkessel model (Burattini et al. 1994; Manning et al. 2002). While useful, the Windkessel model has two limitations: (1) parameters cannot be specified as a function of model geometry; and (2) Windkessel models cannot account for flow and pressure wave changes including damping or amplification and dispersion that occur in a branched network of compliant blood vessels with spatially varying properties (Olufsen and Nadim 2004). An alternate method not subject to these limitations is to compute the impedance using a fractal network (Taylor 1966; Brown 1996; Olufsen 1999) representing the vascular bed. In this work, the objective is to extend the structured tree model developed by Olufsen (1999) to compute the impedances of vascular beds during rest and exercise. Short-term regulatory mechanisms in the body continuously alter the impedance of vascular beds to control the distribution of blood due to varying demands of organs and tissues. These regulatory mechanisms act on the vascular beds resulting in changes in vascular anatomy such as vasodilation or vasoconstriction and recruitment or closure of capillary beds by the opening and closing of pre-capillary sphincters. Following the onset of leg exercise, heart rate (HR) and cardiac output (CO) are increased and as a result, the aortic flow waveform is changed from tri- to bi-phasic as negative flows are eliminated. Impedance in the leg is decreased due to the dilation (3 5 times) of arterioles or recruitment of non-flowing capillaries to meet the metabolic demand of the active muscles. Meanwhile, the vascular beds that supply non-essential organs and inactive muscles reduce flow, using constriction of arterioles or pre-capillary sphincters to direct more of the CO to high-demand locations and maintain blood pressure. These impedance-regulating mechanisms can be incorporated by using geometric alterations in the structured tree impedance boundary. A number of in vitro and numerical studies have been performed to visualize the changes in flow features in the abdominal aorta during exercise (Pedersen et al. 1993; Moore and Ku 1994; Boutouyrie et al. 1998; Taylor et al. 1999b). In these studies, the goal was to understand current hemodynamic conditions with a prescribed, known outflow condition. This method would not be suitable in determining the change in flow features following a change in the geometry of the modelled region. The ability to simulate both rest and exercise is desired because diagnostic data required for modelling is primarily collected with the patient at rest and symptoms of lower extremity vascular disease are most evident during exercise. One of the most pronounced symptoms of lower extremity vascular disease is claudication, pain in the thigh and buttock during exercise due to diminished capacity to deliver blood to active muscle. Currently, the success rate of relieving claudication is not easily predicted as it is related to the location and extent of disease, the ability of proximal vessels to supply blood to the region, and the capacity of distal beds to accommodate runoff. As a consequence of this difficulty, potential negative outcomes include: (1) patient may be required to undergo a re-do operation to relieve symptoms following an under aggressive treatment; (2) patient may not benefit due to being a poor candidate; or (3) patient may suffer unnecessary complications from overaggressive treatment. Computational modelling for surgical planning in the scenario above, with the ability to model the exercise state based on data collected during rest, may improve the success rate and reduce the risk to patients suffering from claudication. In summary, this paper shows how to model blood flow in large vessels during rest and exercise. We demonstrate the effect of changing inlet HR, CO, and the geometry of the structured tree attached at the outlet. This model is validated against non-invasively recorded phase contrastmagnetic resonance imaging (PC-MRI) flow data for eleven healthy subjects during rest and exercise. 2. Methods 2.1 Governing equations Axisymmetric 1D equations for blood flow and pressure can be derived by appropriately integrating the 3D Navier Stokes equations over the vessel cross-section and neglecting in-plane components of velocity (Hughes and Lubliner 1973; Hughes 1974). This model is used to describe large vessels in which the blood flow is considered Newtonian, the fluid is considered incompressible and the vessel walls are assumed to be impermeable. We further assume that the velocity profile across the diameter of the vessels is parabolic (Wan et al. 2002). The resulting partial differential equations for conservation of mass (1) and balance of momentum (2)

4 Fractal network model for rest and exercise 41 are given by: q t þ z 4 q 2 3 s s t þ q z ¼ 0 ð1þ þ s p r z ¼ 28pn q s þ n 2 q z 2 : ð2þ pressure and flow of the form: Pðz; vþ ¼ Zðz; vþqðz; vþ, Qðz; vþ ¼ Pðz; vþyðz; vþ; where Yðz; vþ ¼ 1=Zðz; vþ: ð5þ The primary variables are cross-sectional area sðz; tþ (cm 2 ), volumetric flow rate, qðz; tþ (cm 3 s 21 ), and pressure, pðz; tþ (dynes s 21 cm 22 ); z (cm) is the axial location along the arteries and t (s) is time. The density of the fluid is given by r ¼ 1.06 g cm 23, the kinematic viscosity is given by n ¼ cm 2 s Constitutive equation The above system has three variables, but only two equations. Hence, to complete the system of equations, a constitutive relationship is needed. In this paper, we have used a model that describes pressure p as an elastic function of the cross-sectional area s. This equation, derived by Olufsen (1999), is given by: pðsðz; tþ; z; tþ ¼ p 0 þ 4 Eh 3 r 0 ðzþ s ffiffiffiffiffiffiffiffiffiffiffi! s 0 ðzþ 1 2 sðz; tþ where p 0 is the unstressed pressure, E (g s 22 cm 21 ) is Young s modulus, h (cm) is the thickness of the arterial wall, r 0 (z) (cm) is the radius of the unstressed vessel at location z, and s 0 (cm 2 ) is the cross-sectional area of the unstressed vessel. Young s modulus times the wall thickness over the radius is defined by: Eh r 0 ðzþ ¼ k 1e k 2r 0 ðzþ þ k 3 ; where k 1 ¼ g s 22 cm 21, k 2 ¼ cm 21, and k 3 ¼ g s 22 cm 21 are constants obtained from Olufsen (1999). This elastic model is only an approximation and hence, it does not reflect the viscoelastic nature of arteries. 2.3 Initial and boundary conditions Initially, the cross-sectional area is prescribed from model geometry, and the initial flow is set to zero. Since the above system of equations is hyperbolic, one boundary condition must be specified at each end for all vessels. There are three types of vessel endings: inlets, outlets and bifurcations. At the inlet, we specify a flow waveform qð0; tþ from data, and at the outlets, we use an expression for impedance obtained by solving the linearized version of the Navier Stokes equations in the structured tree using an approach first described by Womersley (1955) and Taylor (1966). The impedance is computed as a function of frequency, v (s 21 ). It provides a relation between ð3þ ð4þ Pðz; vþ; Qðz; vþ; Zðz; vþ and Yðz; vþ are frequency dependent pressure, flow, impedance and admittance, respectively. Since these expressions are applied as outlet conditions, for each outflow vessel they are calculated at z ¼ L: For each outlet, the relation between variables expressed in the time domain and their counterparts in the frequency domain is found using the Fourier transform. Hence, time dependent quantities can be obtained using the convolution theorem, i.e.: qðz; tþ ¼ 1 T ð T=2 2T=2 yðz; t 2 tþpðz; tþ dt; where z ¼ L and y is admittance in the time domain. In our implementation, we evaluate the flow waveform by computing the flow at discrete time points using the form: qðl; nþ ¼ XN21 yðl; jþpðl; n 2 jþ; j¼0 where N is the number of time steps per cardiac cycle and L is the length of the given vessel. Finally, bifurcation conditions are introduced to link properties of a parent vessel x p and daughter vessels x di, i ¼ 1; 2. For each bifurcation three relations must be obtained, an outlet condition for the parent vessel and an inlet condition for each daughter vessel. One equation is obtained by ensuring that flow is conserved, and two other relations are obtained by assuming continuity of pressure: q p ðl; tþ ¼ q d1 ð0; tþ þ q d2 ð0; tþ; p p ðl; tþ ¼ p d1 ð0; tþ ¼ p d2 ð0; tþ: Pressure losses associated with the formation of vortices downstream from the junctions are accommodated for by including a minor loss term applied in the proximal region of the junction vessels. For a detailed description, see Steele et al. (2003). To solve the system of equations (1) (3) combined with the inlet condition, outlet conditions (7), and bifurcation conditions (8), we employ a space-time finite element method that include Galerkin least squares stabilization in space and a discontinuous Galerkin method in time. We use a modified Newton Raphson technique to solve the resultant nonlinear equations for each time step (Wan et al. 2002). ð6þ ð7þ ð8þ

5 42 B. N. Steele et al. 2.4 Impedance boundary condition for vascular networks Vascular impedance is the resistance to blood flow through a vascular network. During steady state (i.e. at rest or during steady exercise), impedance can be computed from the structured trees that represent vascular beds and used as an outlet boundary condition. The vascular impedance at the root of the fractal tree is obtained in a recursive manner starting from the terminal branches where pressure is assumed to be 0 mmhg (figure 2). Along each vessel in the structured tree, impedance is computed from linear, axisymmetric, 1D equations for conservation of mass and momentum (Olufsen et al. 2000). Linearized equations are appropriate for use in arteries with diameter smaller than 2 mm where viscosity dominates (Olufsen and Nadim 2004) and the nonlinear advection effects can be neglected as a first approximation (Womersley 1957; Atabek and Lew 1966; Pedley 1980). The details of this computation are given in Olufsen et al. (2000). Briefly, the input impedance is computed at the beginning of each vessel z ¼ 0 as a function of the impedance at the end of a vessel z ¼ L : Zð0; vþ ¼ ig 21 sin ðvl=cþ þ ZðL; vþcosðvl=cþ cosðvl=cþ þ igzðl; vþsinðvl=cþ p L is vessel length, c ¼ ffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffi s 0 ð1 2 F J Þ=ðrCÞ is the wavepropagation velocity, where: ð9þ Zð0; 0Þ ¼ lim Zð0; vþ ¼ 8ml rr v!0 pr 3 þ ZðL; 0ÞF J ¼ 2 J 1ðw 0 Þ 0 w m J 0 ðw 0 Þ ð10þ J 0 (x) and J 1 (x) are the zero th and first order Bessel functions with w 2 0 ¼ i 3 w and w 2 ¼ r 2 0v=y: The compliance C is approximated as: C < 3s 0r 0 2Eh ; ð11þ 2.5 The geometry of the structured tree The small arteries that form the vascular bed are described using a bifurcating self-similar tree characterized by three parameters as described below (Olufsen 1999). The first parameter describes the branching relationship across bifurcations between the radius of the parent vessel r p and the radii of the daughter vessels r di ; i ¼ 1; 2: There are two methods for defining this relationship, the area ratio and the power law. The area ratio, h, is given by: h ¼ r2 d1 þ r2 d2 r 2 p The power law is defined by: r k p ¼ r k d1 þ r k d2 ð13þ ð14þ If k ¼ 2, then area will be conserved. Murray (1926) studied the physiologic organization of the vascular system and applied the principle of minimum work to examine the correlation between structure and function of the arteries. Murray derived that maximum efficiency for blood flow is attained when the relationship between flow and vessel radius is of the form q / r 3 ; or k ¼ 3: However, observations show that k is neither constant nor organ specific. It has also been suggested that k may vary depending on the radius of the branches involved. Several studies show that k varies from 2 to 3 with mean values given by 2.5, 2.7 and 2.9 (Iberall 1967; Zamir 1999; Karch et al. 2000). Zamir (1999) introduces the concept of sub-ranges of vessels based on diameter. In this work, we adopt this concept and use a three tiered structure with k ¼ 2:5 for small arteries r, 250 mm, k ¼ 2:7 for resistance arteries 250 mm, r, 50 mm; and k ¼ 2:9 for vessels r, 50 mm (table 1). The second parameter, g, is known as the bifurcation index or the asymmetry index. The asymmetry index describes the relative relationship between the daughter vessels: where s 0 is the reference cross-sectional area, Eh=r 0 is defined in equation (4), and g ¼ cc: The impedance at v ¼ 0 can be found as: Zð0; 0Þ ¼ lim v!0 ð12þ where l rr ¼ L=r is the length-to-radius ratio described below and viscosity, m ¼ g cm 21 s. g ¼ r d1 r d2 : ð15þ Assuming that r d1 # r d2 ; g is between 0 and 1. The asymmetry index varies widely throughout vascular beds and does not appear to be organ specific (Papageorgiou et al. 1990; Zamir 1999). We chose to vary the asymmetry ratio in the tiered system described above with g ¼ 0.4, 0.6 and 0.9 (table 1). Table 1. Parameters used to describe the structured tree. The tree is divided into three levels as a function of the vessel radius (second column). For each level, the parameters that describe the power exponent k (third column) and the asymmetry ratio g (last column) are varied. Level Radius Power exponent Asymmetry ratio Small arteries 250 mm, r k ¼ 2.50 g ¼ 0.4 Resistance vessels 50 mm, r, 250 mm k ¼ 2.76 g ¼ 0.6 Capillaries r, 50 mm k ¼ 2.90 g ¼ 0.9

6 Fractal network model for rest and exercise 43 Finally, the length of a given artery (between bifurcations) can be expressed as a function of the mean radius of the vessel. Iberall (1967) recommends the use of a length-to-radius ratio, l r r, of 50. This conclusion was drawn from analysis of data collected in several studies that produced a range of estimates. Zamir (1999) suggests that the mean l r r is 20 with a maximum of 70. Others (Suwa et al. 1963; Iberall 1967; Zamir 1999) have shown that that the l r r in the vascular bed is widely varied and that the value is organ specific. We elect to use l rr as a mechanism to vary the relative impedance between outlets. The properties described above are used in an asymmetric structured tree originally devised by Olufsen (1999). The limbs of the structured tree are systematically ordered to take advantage of pre-computed branches to minimize the computational cost in arriving at the root impedance. In the tree, the radii of successive daughter vessels (r d1 and r d2 ) were obtained by introduction of scaling parameters a and b for the radius of the root vessel (r root ) such that: r d1 ¼ ar root ; r d2 ¼ br root and r i; j ¼ a i b j2i r root ð16þ for the radius of the ith daughter vessel in the jth generation of the tree and i ¼ {0; 1;... ; j} (see figure 1). The power law, area and asymmetry ratios are combined to give: and h ¼ r2 d1 þ r2 d2 r 2 p 1 þ g ¼ ð1 þ g k=2 Þ 2=k ¼ ðar rootþ 2 þ ðbr root Þ 2 r 2 p a ¼ ð1 þ g k=2 Þ 2ð1=kÞ p and b ¼ a ffiffiffi g ð17þ ð18þ Olufsen used an asymmetry ratio g ¼ 0:41; a power k ¼ 2:76 and l r r ¼ 50: The terminal minimum radius ( mm) was used as the mechanism to vary the relative impedance between outlets. In our implementation of the structured tree, we developed a three-tiered model with variables r root and l rr to mimic the behaviour of a specific vascular bed. The tiers in table 1 were developed based on the literature described above and to provide asymmetry. These modifications to Olufsen s structured tree allow the extension of the structured tree to a minimum radius of 3 mm where pressure is set to be 0.0 mmhg. The extension of the structured tree to include resistance vessels facilitates the use of a scaling factor, described below to model physiologic regulation. Although the apparent viscosity of blood in microcirculation is a function of both vessel diameter and hematocrit (Fahraeus-Lindqvist effect) Pries et al. (1990), we do not include this variation in viscosity in this impedance model. 2.6 Modeling exercise During exercise, regional resistance vessels regulate the blood supply to active muscle and non-essential organs. To mimic this behaviour, the radii of the resistance vessels ðr, 300 mmþ were adjusted by a scaling factor, f, to simulate the increase or decrease in effective crosssectional area of the vascular bed during exercise. If f, 1; the effective vessel cross-section is decreased and if f. 1; the effective cross-sectional area is increased. In order to preserve the structure of the tree, the length and radii of the vessels and number of generations of the tree are determined before scaling vessel radii. 2.7 Application with non-invasively measured data The method described above is validated using flow data obtained non-invasively from 11 healthy subjects Taylor et al. (2002). Through-plane flow velocities were acquired using a Cine PC-MRI sequence on a 0.5 T open magnet (GE Signa SP, GE Medical Systems, Milwaukee, WI, USA). To minimize blood flow regulation associated with digestion, subjects were instructed to fast 2 h before scanning. Subjects were seated on a custom built magnetic Table 2. Standard dimensions for idealized model. Columns describe the large vessels in the idealized model with inlet and outlet radii and vessel lengths (cm) Vessel Inlet radius (cm) Outlet radius (cm) Length (cm) Figure 1. Structured tree and sub-unit. The root of the tree is the interface between the modeled domain and boundary condition. Radii of vessels determined using scaling parameter a i b j2i. In the convention shown, the leftmost daughter vessel assumes i ¼ j and the right most daughter vessel assumes i ¼ 0. Aorta Celiac Superior mesenteric Renal Inferior mesenteric Iliac Internal iliac Femoral Profunda

7 44 B. N. Steele et al. Table 3. Estimated flow distribution expressed as a percentage of CO. Structured tree boundary conditions were created for each outlet using the specified length-to-radius ratio. This uniform set of parameters was used for each subject. Target distribution in extremities estimated from measured data and physiologic blood pressure. Symmetry was assumed in right/left-paired vessels (renal, profunda, internal iliac, and femoral). Outlet Target CO (%) Length-to-radius ratio Celiac Superior mesenteric Renal (2) Inferior mesenteric 4 24 Internal iliac (2) 4 60 Profunda (2) 4 60 Femoral (2) 5 80 resonance (MR) compatible cycle ergometer with their torsos in the field of view of the magnet. To minimize movement, the subjects were securely strapped to the seat. PC-MRI images of velocity and cross-section were captured at the supraceliac (SC) and infrarenal (IR) levels of the abdominal aorta at rest and during moderate cycling exercise. The velocities were integrated over the crosssectional area of the vessel to compute the flow rate. The single cardiac cycle produced by PC-MRI is a composite of many gated cardiac cycles. Patient specific anatomy and pressure data were not acquired. In order to develop subject specific geometric models, an existing geometry of an idealized abdominal aorta with major branching vessels (Moore and Ku 1994) was scaled to match measured SC and IR cross-section areas. The idealized model includes one inlet vessel and 11 outlet vessels as shown in figure 2 and in table 2. The scaling was performed using an allometric scaling law (West et al. 1997): R ¼ R 0 M b ð19þ where R represents the desired radius (cm), R 0 is the known scaling constant (cm), M represents the body mass and b is the scaling exponent. The measured cross-sectional area of the aorta for one test subject matched the aorta of the idealized model. This subject was assumed ideal and the SC radius was used as the scaling constant Y 0. This subject s body mass was designated M 0. In order to balance the units, M ¼ M i =M 0 where M i is the test subjects body mass. Using the measured radius from the PC-MRI slice obtained at the SC aorta (R) during diastole, the size of the idealized aorta (R 0 ), and the mass for each subject (M), the scaling exponent, b, was found to be for the measured data. This scaling exponent was then used to scale the idealized model for all remaining vessels. The scaled idealized models represent the large vessels in the computational domain including the aorta (inlet), celiac, superior and inferior mesenteric, renal, iliac, internal iliac, femoral and profunda arteries (see figure 2 and table 2). Next, boundary conditions were determined. The inlet boundary condition for each subject was specified from the SC flow waveforms measured using PC-MRI. All outlet boundary conditions were specified using the modified structured tree with root radius set to the model domain boundary radius and neutral tone, f ¼ 1:0: Initially, visceral outlet boundaries were assigned l rr ¼ 20 and leg outlet boundaries were assigned l rr ¼ 70: The l rr values were adjusted so that the distribution of flow to the viscera matched distributions reported in the literature and to maintain a physiologic blood pressure of approximately 120/70 mmhg (see table 3). For normal, healthy individuals, it is assumed that approximately 20 27% of CO flows through the renal arteries (Ganong 1995) and approximately 27% flows through the celiac, superior mesenteric artery (SMA), and inferior mesenteric artery (IMA) combined. The resting, fasting mesenteric flow can be further estimated as 10% of the CO to the celiac artery, 13% to the SMA, and 4% to the IMA (Ganong 1995; Perko et al. 1998). Because CO was not measured, each subject s blood volume in litres was estimated as 7% of body mass (self reported) in kg. This value was used to estimate resting CO by assuming the entire blood volume is circulated in 1 min (table 4). Using this target CO, l rr values were determined for all outlets (table 3). These outlet boundary conditions were used to perform resting flow analysis for all subjects. Finally, steady exercise was simulated by modifying the boundary conditions. The inlet boundary condition for each subject was specified using the SC flow measured using PC- MRI during exercise. The outlet boundary conditions were modified by specifying a scaling factor, f, as described above. To determine appropriate factors, a series of analyses were performed on one data set. Initially, peripheral beds were dilated by a factor of 5 as described in Ganong (1995) to increase flow to the active muscles Table 4. Estimated resting CO based on weight and HR. Estimated SC aortic flow based on target percentage (66%) of estimated CO. Measured SC flow and error between measured and estimated SC flow. Subject Weight (kg) HR Estimated CO (l min 21 ) Estimated SC (l min 21 ) Measured SC (l min 21 ) Error (%)