TENDER DOCUMENT PURCHASING EQUIPMENT FOR PEPTIDE SYNTHESIS

Transcription

1 TENDER DOCUMENT PURCHASING EQUIPMENT FOR PEPTIDE SYNTHESIS for Barents BioCentre

2 Section 1 Description of the purchase 1.1. Introduction The Northern Research Institute (NORUT) P.O. Box 6434 Forskningsparken, 9294 Tromsø, Norway, organizes Barents BioCentre Purpose Barents BioCentre is requesting Tender for various equipment for peptide synthesis Purchase Depending on the actual financial situation Barents BioCentre plans to buy various equipment for peptide synthesis.

3 Section 2 Conditions of tendering 2.1. Guidelines This purchase will follow an open procedure according to Lov om offentlige anskaffelser of 16. July 1999 no. 69 and Forskrift om offentlige anskaffelser of 7. April 2006 no 402 with subsequent amendments. The tender competition is announced both at and TED database. Tenders shall comply with the instructions given in this document. Any reservations with regard to the terms of contract or other parts of the Tender document shall be clearly marked as such and properly referenced to the relevant part of the tender document Final date for receipt of Tenders Final date for receipt of Tenders is 26 th of September 2011, 1500h (local time) at the latest. Tenders received after this deadline will be rejected 2.3. Delivery of the Tender The tender must be delivered complete in a neutral, sealed enveloped clearly marked: Tender Peptide Synthesis to the following address: Postal address: Barents BioCentre c/o NORUT Postboks 6434 Forskningsparken 9294 Tromsø Norway The Tender cannot be delivered by .

4 2.4. Queries regarding the tender Queries regarding the Tender must be submitted before 2 nd of September, 1500h (local time) at the latest. All queries shall be submitted to Erling Sandsdalen and Johannes Eksteen by Erling.Sandsdalen@norut.no johann.eksteen@uit.no If answered, the querie and its answer will be published in Doffin Tender format The submitted Tender shall be written in English or Norwegian. The Tender shall contain one (1) written version on paper, and one (1) electronic version (CD or memory stick) of the complete tender. The electronic version should preferably be delivered in the pdf format Tender letter The tender letter shall include the following information about the Tenderer: Company name Address Telephone number E mail address Contact person Tender letter shall be duly signed and dated. The letter confirms that the Tenderer has accepted the terms in the Tender document. Any reservations should be clearly marked and properly referenced to the relevant part of the Tender documents Tender opening The tender will be opened within a few days after the deadline. The opening of tenders is not open to the public Validity of Tender The tender shall be remained valid for 90 days after final date for receipt of Tenders.

5 2.8. Alternative tenders and part tenders Alternative Tenders and part Tenders will not be accepted for sections We accept separate Tender for section 5.1 Peptide Synthesizers The Cost of preparing the Tender The Tenderer shall cover the cost of preparing and submitting the Tender.

6 Section 3 Absolute requirements 3.1. Conditions for participations Documentation that should be included within the Tender (strictly for Norwegian Tenderers) 1. Skatteattest for merverdiavgift 2. Skatteattest for annen skatt 3. HMS erklæring Lack of documentation can lead to reject of the tender 3.2. Minimum requirements All items and performance criteria described in the detailed specifications and classified as must are to be considered as minimum requirements. See section five (5)

7 Section 4 Award criteria Barents BioCentre prefers a user friendly and the scientifically and economically most advantageous Tender, evaluated on the basis of: 1. Functionality and Technical performance weight 60% 2. Price weight 25 % 3. Service and training weight 15 %

8 Section 5 Specifications for the instruments 5.1 Peptide Synthesizers 5.2 Freeze Dryer 5.3 Rotary Evaporator 5.4 Chemistry Diaphragm Pump 5.5 Balances (Precision & Analytical)

9 5.1 Peptide Synthesizers Specifications Automatic multiple peptide synthesizer APS1.00 APS1.01 APS2.00 APS2.01 APS2.02 APS2.03 APS3.00 APS3.01 APS3.02 APS3.03 APS3.04 APS3.05 APS3.06 APS3.07 APS4.00 APS4.01 APS4.02 APS4.03 APS4.04 Vendor The instrument must be produced and distributed by a reputable and trusted company and must be supported by a reliable service team. Software and Operating System The instrument must be completely automated and all operations should be controlled via a computer. The software should be user friendly and flexible enough to develop custom synthesis protocols. A failure response system (i.e. notification of synthesis stoppages via e mail/sms as well as an emergency resin wash feature) are required. Chemistry The instrument must have the capability to synthesize at least six peptides in parallel. A wide synthesis scale range is important (i.e. mole to mmole scale). The instrument should be able to perform both Fmoc and Boc chemistries. Amino acid activations should be done in situ under an inert atmosphere. There should be at least 27 amino acid positions (which are kept under an inert atmosphere). At least eight solvent reservoirs with separate solvent lines are required. Automated peptide cleavage from the resin and automated collection of cleaved peptide crudes should be an available option. Reaction Vessels To facilitate the aforementioned (APS3.02) synthesis scales, disposable reaction vessels of different sizes (e.g. 10mL or 45mL vessels) should be available for use. The reaction vessels should constantly be kept under an inert atmosphere such as nitrogen. After each reaction step the reaction vessels should be washed in an efficient top down manner. Resin agitation should be done with adjustable nitrogen bubbling.

10 APS5.00 APS5.01 APS5.02 APS5.03 APS5.04 Fluidics and Waste System The delivery of reagents should be very accurate and should be done by fixed loop measurement controlled by optical sensors Fluid transfer should be done by positive pressure with an inert gas such nitrogen The instrument should have separate fluid paths for reagents, cleaved peptides and waste solvents. Waste overflow detection must be included Specifications Manual Microwave Assisted Peptide Synthesizer MMS1.00 MMS1.01 MMS2.00 MMS2.01 MMS2.02 MMS2.03 MMS3.00 MMS3.01 MMS3.02 MMS4.00 MMS4.01 MMS4.02 MMS5.00 MMS5.01 MMS5.02 Vendor The instrument must be produced and distributed by a reputable and trusted company and must be supported by a reliable service team. Microwave Technology and Design The instrument should utilize microwave energy suitable for the complete peptide synthesis process The reaction temperature should be measured and controlled by in situ fibre optic probe. The instumuent design should be modular, i.e. the manual system should be able to be upgraded to an automated system at a later stage. Chemistry A wide synthesis scale range is important, i.e to 1.0 mmole. Pre activation of amino acids is required Reaction Vessels The instrument should use disposable reaction vessels Agitation should be done by magnetic stirring Fluidics and Waste System Transfer of reagents to the reaction vessel should be done manually. A vacuum manifold to transfer reagents and washing solvents to a waste containing should be included

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12 5.2 Freeze Dryer Specifications Freeze Dryer FD1.00 FD1.01 FD1.02 FDS2.00 Vendor The instrument must be produced by a reputable and trusted company and should be supported by a reliable service team. The distributor of the product chosen must be the manufacturer s preferred company. Collector Unit FD2.01 The instrument should have a minimum ice holding capacity of 4.5L FD2.02 FD2.03 FD2.04 FD2.05 FD3.00 FD3.01 FD4.00 FD4.01 FD4.02 FD4.03 FD4.04 The collector chamber should be fixed to the instrument, be made of stainless steel and should have at least 12 ports. In order to collect solvents with low eutectic points (such as acetonitrile) the collector temperature must be able to go as low as 84⁰C. It should be able to remove at least 4L of water in 24hours. The refrigeration system should be CFC/HCFC free. System Display The instrument should have a LCD display for operating parameters and alarm messages Miscellaneous It should have a vacuum control/break valve to protect the system from oil backstreaming. It should have an automatic start up switch for collector cool down and vacuum pulldown. A retractable collector drain line to remove captured solvents after defrosting. A compact console to house the vacuum pump in.

13 5.3 Rotary Evaporator Specifications Rotary Evaporator RE1.00 RE1.01 RE1.02 RE2.00 RE2.01 RE2.02 RE2.03 RE2.04 FD3.00 RE3.01 RE3.02 RE3.03 FD4.00 RE4.01 Vendor The instrument must be produced by a reputable and trusted company and should be supported by a reliable service team. The distributor of the product chosen must be the manufacturer s preferred company. Temperature control The heating capacity should be 1300W The temperature range for the instrument should be C and accuracy for the bath must be ± 1 C. The heating bath control should be electronic/digital and must be displayed on a LCD The heating bath should have a diameter of 255mm, a volume of 4.5L and should be made from stainless steel. Rotation The rotator s height adjustment should be manual. Rotation speed should be rpm s and the speed setting should be displayed on a LCD The drive should be a brushless DC Miscellaneous Height adjustment for the rotator should be at least 155mm RE4.02 The heating bath should have a secondary overtemperature cut off at 250 C RE4.03 The instrument should have a remote control function to start rotation and heating.

14 5.4 Chemical Diaphragm Pump Specifications Chemical Diaphragm Pump CDP1.00 CDP1.01 CDP 1.02 CDP2.00 CDP2.01 Vendor The instrument must be produced by a reputable and trusted company and should be supported by a reliable service team. The distributor of the product chosen must be the manufacturer s preferred company. Vacuum pump The pump should have heads/stages CDP2.02 The maximum pumping speed should be at least 2 m 3 /h CDP2.03 The ultimate vacuum should be at least 7 mbar CDP2.04 The motor power should be 0.18kW and the speed (at 50Hz) should be 1500 min 1. CDP3.00 CDP3.01 CDP3.02 CDP4.00 CDP4.01 Inlet and outlet The inlet should have a separator made of glass with a protective coating to retain particles and liquid droplets The outlet should have a highly efficient waste vapor condenser to enable solvent recovery and efficient recycling. Miscellaneous Outstanding chemical resistance and vapor tolerance is required

15 5.5 Balances Specifications Precision Balance PB1.00 PB1.01 PB 1.02 PB2.00 PB2.01 Vendor The instrument must be produced by a reputable and trusted company and should be supported by a reliable service team. The distributor of the product chosen must be the manufacturer s preferred company. Measurements and Display The maximum measuring range should be 220g and a capacity display funtion must show how much of the weighing range is still available. PB2.02 The LCD readout should be in 0.001g and stabilization time 3s. PB2.03 PB3.00 PB3.01 PB4.00 PB4.01 The reproducibility should be 0.001g and the linearity ± 0.002g. Calibration The instrument should be capable of performing interal calibration. Miscellaneous The instrument should possess a leveling foot and a draft guard. Specifications Analytical Balance PB1.00 PB1.01 PB 1.02 PB2.00 PB2.01 Vendor The instrument should be produced by a reputable and trusted company and should be supported by a reliable service team. The distributor of the product chosen should be the manufacturer s preferred company. Measurements and Display The maximum measuring range should be 220g and a capacity display funtion must show how much of the weighing range is still available. PB2.02 The LCD readout should be in g and stabilization time 5s. PB2.03 The reproducibility should be g and the linearity ± g.

16 PB3.00 PB3.01 PB4.00 PB4.01 Calibration The instrument should be capable of performing interal calibration. Miscellaneous The instrument should possess a leveling foot and a draft guard. Section 6 Delivery terms All products and services covered by the tender shall be supplied free of charge, delivered to Barents BioCentre. Free of charge is to be interpreted as DDP in accordance with Incoterms 2000