Advanced Course Downstream Processing

Transcription

1 Advanced Course Downstream Processing 4-8 July, 2016 Aim The challenge for biochemical engineers is to design compact and clean processes to efficiently separate instable products, such as recombinant proteins, from dilute complex fermentation broths to the required pharmaceutical degree of purity. Therefore, the quantitative systematic design of integrated downstream processes is the general theme of this Advanced Course. The aim of the course is to provide the tools for the participants to be able to quantitatively and systematically design integrated downstream processes. Course description The course presents the state of the art in downstream processing of biotechnological products. It provides knowledge of different techniques for solid-liquid separation, product release, refolding, concentration and purification of precious biotechnology products with a focus on the total integrated process. An international group of experts from both industry and academia will lecture to assure an optimal balance of practical knowledge and theoretical insight, which subsequently will be trained by exercises, into the quantitative engineering aspects of bioseparations. At the end of the course, the participants should be able to estimate main equipment dimensions, know qualitative constraints to the integrated bioprocess and have a quantitative insight in process streams, structure and economics. The course starts with an overview of possibilities and problems typically associated with the recovery of bioproducts. Physico-chemical characteristics of the products, as well as the fermentation broth with its multiple contaminants, are discussed in relation to possibilities for selective separation. Using this information, the general structure of large scale industrial processes will be outlined. The thermodynamical basics and biochemical engineering principles will be introduced and developed during the course related to bio-separations. Special attention will be paid to the unit operations typically used in this field of industry. Firstly, various solid-liquid separation techniques and cell disruption will be treated. Particular consideration will be given to the application of major concentration techniques such as extraction, adsorption and membrane separation. Furthermore, the scientific and industrial aspects of purification by means of precipitation and chromatography will be discussed. In addition, the release of intracellular products as well as protein folding in industrial protein processes will be discussed. Process integration is the binding element in the course. During the course, a case study on the design of an integrated purification process for a recombinant protein will offer the participants the opportunity to practice on the individual unit operations as well as on the integrated process. Finally, possibilities of computer-aided, rational design of integrated separation processes will be demonstrated. This intensive course consists of theoretical and applied lectures, exercises, case studies and computer simulations. The course will be given in English. A laptop and pocket calculator is required.

2 Who should attend? This Advanced Course is aimed at professionals (MSc or PhD level is required) in (bio)chemical engineering, or in microbiology or biochemistry with a basic knowledge in chemical engineering. The course is primarily aimed at those already employed in industry and who are interested in the separation of biotechnological products. In addition, this Advanced Course is an option in the two-year postgraduate programs of. Duration / Location This Advanced Course will be given on Monday, 4 July Friday, 8 July 2016 The course will be held at: Hampshire Hotel Delft Centre Koepoortplaats RR Delft the Netherlands P +31 (0) Contact course organisation Department of Biotechnology Julianalaan BC Delft the Netherlands P E bsdl-edu@tudelft.nl W Accommodation The organising committee has blocked a sufficient number of hotel rooms at Hampshire Hotel Delft Centre. The costs of bed/breakfast are approx. 99,50 (single room, inclusive breakfast). Participants have to book their own room. Hotel reservation is handled by Hampshire Hotel Delft Centre. Participants can book their hotel room by sending an to: meeting@hoteldelftcentre.nl or by telephone: +31 (0) Please mention booking code ACDP2016. Lunches, the buffet on Monday, July 4 th and the course dinner on Thursday, July 7 th will be provided. For the other meals a variety of restaurants can be found in the centre of the city.

3 Program, 4 8 July, 2016 Monday, July 4 th Themes: Today s bioseparation processes Mechanical separations: solid-liquid separation Introduction to the separation processes Pitfalls and challenges in bioseparation processes Luuk van der Wielen Engineering fundamentals Mass balances: from batch to continuous counter current processes Luuk van der Wielen Case study: industrial protein production (I) Introduction to the case study Splitting up into teams working on the case Mass balances Solid-liquid separation: filtration and centrifugation Assignment: design of filtration equipment Predicting molecular properties Molecular properties and selection of separation conditions Equilibrium calculations Luuk van der Wielen Case study: industrial protein production (II) Getting familiar with the case study and investigating options for improvement of a protein purification process Social event and buffet Tuesday, July 5 th Themes: Mechanical separations: solid liquid separation Concentration and design Centrifugation in the biopharmaceutical industry Design considerations of sedimentation and centrifugation processes: from biomass removal to centrifugal extraction Alex Hanke Centrifugation assignment Extractive separation Solvent selection and equipment design in extraction processes Extraction assignment Membrane technology: theory, design & industrial application Reinoud Noordman Assignment: membrane separation Case study: industrial protein production (III) Engineering and design of unit operations

4 Wednesday, July 6 th Theme: Purification processes Design of chromatographic separations Equilibrium theory and column design Non-linear and mass transfer effects Chromatography in industrial practice Jan-Christer Janson Industrial cases and applications of chromatography Column design and operation from theory and practice Jeroen den Hollander Chromatography assignment Continuous chromatography and Simulated Moving Bed (SMB) Technology Practical aspects and implementation for large scale chromatography in industry Marc Bisschops Case study: industrial protein production (IV) Engineering and design of unit operations Lab tour: High throughput facilities (optional) Marcelo Silva / Silvia Pirrung Thursday, July 7th Theme: Purification & formulation processes Quality by design Danielle Horneman Bulk crystallization Unit operation design for the crystallization of small and large biomolecules Crystallization assignment Industrial crystallization Real life examples Rob Geertman Inclusion body processing and refolding Michel Eppink Design of an industrial process for purification of biologicals Michel Eppink Case study: industrial protein production (V) Process design: sequencing and interfacing of unit operations Course dinner Friday, July 8 th Theme: Process development & process integration Rational methodology for protein purification Beckley Nfor High Throughput techniques in downstream process development Jürgen Hubbuch Process integration in industry Arne Staby Case study: industrial protein production (VI) Presentations of the three winning teams: the strategy DSP award ceremony Evaluation of the course and farewell drink

5 Fees and Registration Please fill in the application form on our website or complete and return the form below if you are interested to attend the course or would like to receive information on other courses. Applications will be handled in order of the date of receipt. The course fee is: Early bird fee: in case of payment received before May 23 rd, 2016 or Regular fee: in case of payment received after this date. In the event of your cancellation before May 23 rd, 2016 a full refund will be granted, after this date, a 25% fee charge will be made. To facilitate enrolment of young PhD-students, a limited number of fellowships is available. The course fee with fellowship is To apply, please include a copy of your enrolment as a PhDstudent from your university. The fee includes course materials, lunches, the buffet on Monday, July 4 th and the course dinner on Thursday, July 7 th. The fee does not cover other meals and lodging. When the number of participants is too low to have a fruitful course, the Institute BSDL will cancel the event no later than six weeks before the start of the course. The course fee will be reimbursed within three weeks after cancellation. In case a speaker will not be able to present his/her lecture, due to unforeseen circumstances, BSDL will arrange an equivalent replacement. Hotel accommodation can be arranged at your request directly at Hampshire Hotel Delft Centre. Preparatory texts will be sent after receipt of the course fee. The complete course book will be supplied at the start of the course. Advanced Course Downstream Processing o I wish to attend the course of 4 8 July, 2016 o I would like to receive information of the other courses of the Institute BSDL o Please, send me announcements of the future Advanced Course Downstream Processing Family name, title, Mr/Ms. First name.. Organisation/Company. Address. Phone. address.. Educational background Diet wishes Date / Signature

6 Board of the course Dr. M. Ottens Prof. L.A.M. van der Wielen Prof. M.H.M. Eppink Synthon BV Nijmegen, the Netherlands Prof. J. Hubbuch Karlsruhe Institute for Technology (KIT) Karlsruhe, Germany Dr. E.J.A.X. van de Sandt DSM Anti-Infectives Dr. A. Staby NOVO Nordisk A/S Gentofte, Denmark Dr. J. Thömmes Biogen Idec Inc. San Diego, USA Lecturers Dr. M.A.T. Bisschops Batavia BioServices B.V. Leiden, the Netherlands Prof. M.H.M. Eppink Synthon BV Nijmegen, the Netherlands Dr. R. Geertman DSM Fibre Intermediates BV Geleen, the Netherlands Dr. A.T. Hanke Novartis Basel, Switzerland Dr. D. Horneman Batavia BioServices B.V. Leiden, the Netherlands Prof. J. Hubbuch Karlsruhe Institute for Technology (KIT) Karlsruhe, Germany Prof. J.-C. Janson Uppsala Biomedical Centre Department of Surface Biotechnology Uppsala, Sweden Dr. B. Kungah Nfor Crucell Leiden, the Netherlands Dr. R. Noordman Pentair X-Flow Enschede, the Netherlands Dr. M. Ottens S.M. Pirrung Dr. A. Staby NOVO Nordisk A/S Gentofte, Denmark Prof. L.A.M. van der Wielen Course coordinators Ms. Jenny Boks-Zondervan Mr. Vincent Renken, MSc MSc(Ed) Department of Biotechnology M.D. Henriques da Silva Dr. J.L. den Hollander DSM Biotechnology Center

7 Institute Biotechnology Studies Delft Leiden Department of Biotechnology Julianalaan BC Delft The Netherlands The institute Biotechnology Sciences Delft Leiden (BSDL-EDU) constitutes a joint initiative in biotechnological post-graduate education of and Leiden University and is coordinated from the department of Biotechnology of. BSDL-EDU was founded in 1987 and has since then very successfully organised various types of postdoctoral education: the Advanced Course Quality Management in Pharma and Biotech, the PDEng programmes and the Advanced Courses in biotechnology. The Advanced Course Quality Management in Pharma and Biotech was developed by BSDL-EDU and is currently organised by PAO Farmacie. The PDEng programmes are special two-year postgraduate programmes that are aimed at those who wish to tailor their own specialisation to the needs of multidisciplinary biotechnological research and design, and lead to the degree of Professional Doctorate in Engineering. Originally developed by BSDL-EDU, these programmes are now hosted by the 3TU School for Technological Design / Stan Ackermans Institute. Currently BSDL-EDU offers various Advanced Courses covering the multidisciplinary spectrum of biotechnology: MICROBIAL PHYSIOLOGY AND FERMENTATION TECHNOLOGY BIOCATALYSIS AND PROTEIN ENGINEERING BIOPROCESS DESIGN ENVIRONMENTAL BIOTECHNOLOGY GENOMICS IN INDUSTRIAL BIOTECHNOLOGY METABOLOMICS FOR MICROBIAL SYSTEMS BIOLOGY Further information Ms. Jenny Boks-Zondervan Mr. Vincent Renken, MSc MSc(Ed) P E bsdl-edu@tudelft.nl W Address Institute Biotechnology Studies Delft Leiden Department of Biotechnology, Julianalaan 67, 2628 BC