Aldosterone and mineralocorticoid receptor (ADMIRE) COST Action BM1301. Tenerife Training school

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1 Aldosterone and mineralocorticoid receptor (ADMIRE) COST Action BM1301 Tenerife Training school February 9 th -13 th, 2015 Mineralocorticoid receptor cell biology and pharmacology

2 The Aldosterone and Mineralocorticoid Receptor (ADMIRE) COST action BM1301 has organized a training school on mineralocorticoid receptor (MR) pharmacology and cell biology aimed at Early Stage Researchers (ESR: PhD students and postdocs up to eight years after obtaining a PhD degree) from groups participating in the action. In this COST training school trainees will get hands-on experience in searching for new small-molecule MR modulators and characterizing their actions on different levels of the receptor s biology, including gene transactivation, subcellular trafficking, protein-protein and protein- DNA interactions. Faculty Dr. Diego Alvarez de la Rosa (Training School organizer). Dept. Physiology, University of La Laguna (ULL) and Center for Biomedical Research of the Canary Islands (CIBICAN), Tenerife, Spain. Dr. Nourdine Faresse. Dept. of Anatomy, University of Zurich, Switzerland. Dr. Miguel X. Fernandes. Institute of Bioorganic Chemistry (IUBO) and Center for Biomedical Research of the Canary Islands (CIBICAN), Tenerife, Spain. Dr. Rosario González Muñiz. Institute of Medicinal Chemistry, CSIC, Spain. Dr. Claudia Grossmann. University of Halle, Germany. Dr. Antonio Hernandez Daranas. Institute of Bioorganic Chemistry (IUBO) and Center for Biomedical Research of the Canary Islands (CIBICAN), Tenerife, Spain. Dr. Mercedes Martín Martínez, Institute of Medicinal Chemistry, CSIC, Spain. Dr. Araceli Morales. Dept. Physiology, University of La Laguna (ULL) and Center for Biomedical Research of the Canary Islands (CIBICAN), Tenerife, Spain. Applications for the training school Send applications for the training school by to Sheeona Gorman and Diego Alvarez de la Rosa including the following information: Last name, first name, , institution, name of Principal Investigator. Letter of motivation: state why you want to participate in this training school. Support letter from Principal Investigator indicating how attending the training school will benefit the trainee. Abstract (max. 250 words): brief summary of the work of the ERS that will be presented in a short 10 min oral communication to the rest of the trainees during the training school. Deadline for applications: December 3 rd 2014 Places are limited to 10 trainees so be sure to register early! Accepted applications will be announced by December 5 th, 2014

3 Financial support Early stage researchers accepted for the training school will obtain financial support from ADMIRE COST BM1301 that will cover: - Housing for six nights in a University of La Laguna Residence (February 8 th -14 th 2015) - Lunch and dinner for every day of the course - Travel (max 300) - Registration to the course, including coffee breaks PRELIMINARY PROGRAM Monday February 9 th 9:00 Welcome and Introduction to the course (Diego Alvarez de la Rosa, ULL) 9:15-10:15 In silico analysis of potential small-molecule MR inhibitors (Rosario González Muñiz, CSIC, Madrid) 10:15-10:45 Coffee break 10:45-13:00 Practical session part I computation of small molecules interactions with known MR ligand binding domain structures. (Miguel X. Fernandes, ULL; Mercedes Martín, CSIC, Madrid) 14:00-16:00 Practical session part II computation of small molecules interactions with known MR ligand binding domain structures. (Miguel X. Fernandes, ULL; Mercedes Martín, CSIC, Madrid) 16:00-17:00 Analysis of small-molecule binding to purified MR ligand-binding domain by calorimetry and surface plasmon resonance. (Rosario Gonzalez-Muñiz, CSIC, Madrid; Antonio Hernández-Daranas, ULL). 17:00-18:00 Practical demonstration of analysis of small-molecule binding to purified proteins by calorimetry. (Antonio Hernández-Daranas, ULL) Tuesday February 10 th 9:00-10:00 In-cell screening for new MR modulators. Screening strategies. Primary and secondary screenings (Diego Alvarez de la Rosa, ULL)

4 10:00-11:00 Practical session Primary screening of MR modulators using reporter genes and luminescence. Data analysis. (Diego Alvarez de la Rosa, ULL) 11:00-17:00 Free time visit to Teide National Park 17:00-18:00 Tools to study MR cellular dynamics. (Claudia Grossman, University of Halle) Wednesday February 11 th 9:00-10:30 Practical session part I studying the kinetics of MR nuclear translocation using confocal microscopy; pulse-chase analysis of MR dynamics using FlAsH-tagged MR; quantitative analysis of data (Claudia Grossmann, Halle, Germany; Araceli Morales, ULL, Spain). 10:30-11:00 Coffee break 11:00-13:00 Practical session part II studying the kinetics of MR nuclear translocation using confocal microscopy; pulse-chase analysis of MR dynamics using FlAsH-tagged MR; quantitative analysis of data (Claudia Grossmann, Halle, Germany; Araceli Morales, ULL, Spain). 14:00-15:00 Studying MR post-translational modifications (Nourdine Faresse, Zurich) 15:00-17:00 Practical session (part I) analyzing MR protein-protein interactions and post-translational modifications using proximity ligation assay (PLA) (Nourdine Faresse, Zurich; Diego Alvarez de la Rosa, ULL) Thursday February 12 th 9:00-10:30 Practical session (part II) analyzing MR protein-protein interactions and post-translational modifications using proximity ligation assay (PLA) (Nourdine Faresse, Zurich; Diego Alvarez de la Rosa, ULL) 10:30-11:00 Coffee break 11:00-12:00 continuation of practical session (part II) analyzing MR protein-protein interactions and posttranslational modifications using proximity ligation assay (PLA) (Nourdine Faresse, Zurich; Diego Alvarez de la Rosa, ULL)

5 12:00-13:00 Methodological approaches to study MR-DNA interactions. (Dr. Diego Alvarez de la Rosa, ULL, Spain) 14:00-16:00 Practical session (part III) analyzing MR protein-protein interactions and post-translational modifications using proximity ligation assay (PLA) (confocal microscopy; data analysis) (Nourdine Faresse, Zurich; Diego Alvarez de la Rosa, ULL) 16:00-18:00 Practical session (part I) Chromatin immunoprecipitation (sample preparation and processing) (Dr. Diego Alvarez de la Rosa, ULL) Friday February 13 th 9:00-11:00 Practical session (part II) Chromatin immunoprecipitation (part II: continue sample preparation and processing) (Dr. Diego Alvarez de la Rosa, ULL 11:00-11:30 Coffee break 11:00-13:30 continue Practical session (part II) Chromatin immunoprecipitation (qpcr and analysis of results) (Dr. Diego Alvarez de la Rosa, ULL 13:30-14:30 Lunch 14:30-17:00 Short oral presentations by attendees 20:00 FAREWELL DINNER

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