[ WELCOME ] 2015 Waters Corporation 1
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![[ WELCOME ] 2015](/docs-images/32/15705671/images/1-0.png "Waters Corporation")
1 [ WELCOME ] 2015 Waters Corporation 1
2 Lance Nicolaysen Senior Director Americas Mass Spectrometry Business Waters Corporation, USA 2015 Waters Corporation 2
3 MS-Related Job Opportunities Field Marketing Manager, Health Sciences (LC/MS MA Clinical / Forensic / Toxicology) Mass Spectrometry Sales Specialist WA Chemistry Sales Specialist NJ Field Sales Account Manager NC Field Service Engineer NJ, TX, MD, CA, PA, MD, VA, LA Inside Instrument Sales Specialist MA Inside Sales Specialist - Chemistry Consumables MA Mass Spectrometry Clinical Specialist - West District CA Mass Spectrometry Sales Specialist Brazil North District Business Development Manager MA Regional Service Manager CA Senior MS Applications Chemist MA SFC/SFE Field Service Engineer CA SFC/SFE Sales Specialist USA Waters Corporation 3
4 Gordon Kearney Senior Manager Mass Spectrometry Product Management Waters Corporation, UK 2015 Waters Corporation 4
5 What s New from Waters: 2015 Waters Users Meeting 63rd ASMS Conference, St. Louis, MO 30 th May Waters Corporation 5
6 Set up theme Innovation Performance Access 2015 Waters Corporation 6
7 Separations science Mass spectrometry Standards and reagents Informatics Chemistries 2015 Waters Corporation 7
8 Oasis PRiME HLB How can Oasis PRiME HLB simplify sample preparation? How does it compare to other sample preparation products? Sales Tools! Questions? 2015 Waters Corporation 8
9 GlycoWorks RapiFluor-MS N-Glycan Kit Come Visit to Play: 2015 Waters Corporation 9
10 ACQUITY UPLC Glycoprotein BEH Amide 300Å 1.7 µm 2015 Waters Corporation 10
11 NuGenesis LMS 2015 Waters Corporation 11
12 Progenesis QI v2.1 New features for ASMS 2015 More compound databases Greater choice for successful compound ID NIST LC-MS/MS libraries ChemSpider results refinement Theoretical fragmentation Elemental composition filtering Increased confidence in results Pathways analysis Placing discoveries in biological context IPA (Ingenuity / Qiagen) Breakfast Meeting Wednesday Book your place now! 2015 Waters Corporation 12
13 UNIFI UNIFI 1.8 Streamlined Instrument Controls Seamless Workflows Integrated Ion Mobility Tools Sophisticated Reporting UNIFI: Taking Data to Knowledge 2015 Waters Corporation 13
14 The Evolution of Ion Mobility MS 2015 Waters Corporation 14
15 2013 Synapt G2Si HDMS Enhancing Workflows (implementing CCS) Dedicated IMS Apps Software (UNIFI TM ), MS Compare, Intellistart TM 2009 Synapt G2 HDMS 2 nd Gen TriWave & QuanTOF 2011 Synapt G2S HDMS StepWave 2010 MALDI Synapt G2 HDMS HDI Software, ETD 2007 MALDI Synapt 2003 First T-Wave IMS 2006 Synapt HDMS TriWave, DriftScope 2004 Prototype SYNAPT Q-IMS-Tof 2001 Linear Field IMS 2015 Waters Corporation 15
16 Storage Region Gate Electric Field Second Transfer Region Quadrupole Mass Filter Collision Cell Lenses Pusher Detector Ions Electrospray Tip Mobility Region First Transfer Region Stacked Ring Ion Guide Reflectron 2015 Waters Corporation 16
17 2013 Synapt G2Si HDMS Enhancing Workflows (implementing CCS) Dedicated IMS Apps Software (UNIFI TM ), MS Compare, Intellistart TM 2009 Synapt G2 HDMS 2 nd Gen TriWave & QuanTOF 2011 Synapt G2S HDMS StepWave 2010 MALDI Synapt G2 HDMS HDI Software, ETD 2007 MALDI Synapt 2003 First T-Wave IMS 2006 Synapt HDMS TriWave, DriftScope 2004 Prototype SYNAPT Q-IMS-Tof 2001 Linear Field IMS 2015 Waters Corporation 17
18 2015 Waters Corporation 18
19 2013 Synapt G2Si HDMS Enhancing Workflows (implementing CCS) Dedicated IMS Apps Software (UNIFI TM ), MS Compare, Intellistart TM 2009 Synapt G2 HDMS 2 nd Gen TriWave & QuanTOF 2011 Synapt G2S HDMS StepWave 2010 MALDI Synapt G2 HDMS HDI Software, ETD 2007 MALDI Synapt 2003 First T-Wave IMS 2006 Synapt HDMS TriWave, DriftScope 2004 Prototype SYNAPT Q-IMS-Tof 2001 Linear Field IMS 2015 Waters Corporation 19
20 2015 Waters Corporation 20
21 The Challenge of Ion Mobility 2015 Waters Corporation 21
22 2013 Synapt G2Si HDMS Enhancing Workflows (implementing CCS) Dedicated IMS Apps Software (UNIFI TM ), MS Compare, Intellistart TM 2009 Synapt G2 HDMS 2 nd Gen TriWave & QuanTOF 2011 Synapt G2S HDMS StepWave 2010 MALDI Synapt G2 HDMS HDI Software, ETD 2007 MALDI Synapt 2003 First T-Wave IMS 2006 Synapt HDMS TriWave, DriftScope 2004 Prototype SYNAPT Q-IMS-Tof 2001 Linear Field IMS 2015 Waters Corporation 22
23 2015 Waters Corporation 23
24 2013 Synapt G2Si HDMS Enhancing Workflows (implementing CCS) Dedicated IMS Apps Software (UNIFI TM ), MS Compare, Intellistart TM 2009 Synapt G2 HDMS 2 nd Gen TriWave & QuanTOF 2011 Synapt G2S HDMS StepWave 2010 MALDI Synapt G2 HDMS HDI Software, ETD 2007 MALDI Synapt 2003 First T-Wave IMS 2006 Synapt HDMS TriWave, DriftScope 2004 Prototype SYNAPT Q-IMS-Tof 2001 Linear Field IMS 2015 Waters Corporation 24
25 2015 Waters Corporation 25
26 2013 Synapt G2Si HDMS Enhancing Workflows (implementing CCS) Dedicated IMS Apps Software (UNIFI TM ), MS Compare, Intellistart TM 2009 Synapt G2 HDMS 2 nd Gen TriWave & QuanTOF 2011 Synapt G2S HDMS StepWave 2010 MALDI Synapt G2 HDMS HDI Software, ETD 2007 MALDI Synapt 2003 First T-Wave IMS 2006 Synapt HDMS TriWave, DriftScope 2004 Prototype SYNAPT Q-IMS-Tof 2001 Linear Field IMS 2015 Waters Corporation 26
27 Structure Separation CCS Specificity Sensitivity 2015 Waters Corporation 27
28 Opportunity Measure CCS of every ion in every analysis Incorporate CCS into routine workflows 2015 Waters Corporation 28
29 2013 Synapt G2Si HDMS Enhancing Workflows (implementing CCS) Dedicated IMS Apps Software (UNIFI TM ), MS Compare, Intellistart TM 2015 Accessible Ion Mobility 2009 Synapt G2 HDMS 2 nd Gen TriWave & QuanTOF 2011 Synapt G2S HDMS StepWave 2010 MALDI Synapt G2 HDMS HDI Software, ETD 2007 MALDI Synapt 2003 First T-Wave IMS 2006 Synapt HDMS TriWave, DriftScope 2004 Prototype SYNAPT Q-IMS-Tof 2001 Linear Field IMS 2015 Waters Corporation 29
30 Beyond Resolution 2015 Waters Corporation 30
31 2015 Waters Corporation 31
32 QuanTof2 Detector Dual Channel 7.2GHz (up-sampled) 10-bit ADC Amplifier ADC From MS x 1 x 0.03 ADC High Gain Channel ADC Low Gain Channel Peak Processing Peak processing Apply calibration Combine ADC Test signal 2015 Waters Corporation 32
33 Benefits of QuanTof Waters Corporation 33
34 Intensity High Selectivity (HS) MS/MS m/z RT DT m/z m/z DT DT 2015 Waters Corporation 34
35 Routine ion mobility Seamlessly from sample to answers Just press START Simply Acquire Get to the Answers 2015 Waters Corporation 35
36 Benefits of Routine Ion Mobility Clarity cleaner mass spectra Confidence valuable CCS information for every ion System Productivity with UNIFI analytical workflows 2015 Waters Corporation 36
37 Benefits of Routine Ion Mobility Clarity m/z Å 2 Verapamil m/z Unlimited Product ion HDMS E m/z m/z 2015 Waters Corporation 37
38 Vion measured CCS(N 2 ) values <2% time after time Reserpine m/z : 252 Å % RSD LueEnk m/z : 230 Å % RSD Terfenadine m/z : 230 Å % RSD Verapamil m/z : 211 Å % RSD Sulphadimethoxine m/z : 168 Å % RSD Caffine m/z : 136 Å % RSD Data from 400 injections over 33 hours 2015 Waters Corporation 38
39 Acetamiprid Azoxystrobin Bromuconazole II Carbetamide Carbofuran Chlorbromuron Chloridazon (Pyrazon) Clethodim I Clethodim I fgt Clethodim II Clethodim II fgt Cyromazine DEET Demeton-S-methyl-sulfone Dichlorvos Diethofencarb fgt Disulfoton-sulfone Diuron Ethoprophos Fenamiphos sulphone Fenoxycarb Fenoxycarb fgt Fenpropimorph Fenpyroximat Flutriafol Formetanate Fosthiazate Furathiocarb Heptenophos Hexazinone Indoxacarb Isoxaben Linuron Mesotrione Metalaxyl Methabenzthiazuron Metobromuron Metolachlor Metribuzin Mevinphos trans Nitenpyram Oxadiazon pethoxamid phenthoate Pirimicarb Primicarb-desmethyl fgt* Propamocarb propargite Prosulfocarb Pymetrozine Pyraclostrobin Pyridaben Pyridaben fgt 1 Pyridate Pyriproxifen Simazine Spinosad Spirodiclofen Spiroxamine II Tebuconazole tepraloxydim Thiacloprid Thiodicarb Thiophanate-methyl Triasulfuron Triflumuron Trinexapac-ethyl Triticonazole Vamidothion % CCS Difference WIV ISP Study: SYNAPT/Vion Comparison for Pesticides Confidence 5 Synapt WIV ISP/Vion IMS Q ToF Comparison April Pesticide Dr Severine Goscinny, Scientific Institute of Public Health, Belgium Axis Title At Waters user meeting 2015 Waters Corporation 39
40 Benefits of Routine Ion Mobility Confidence 2015 Waters Corporation 40
41 153 Coeluting Components Resolved with Vion Mobility System Productivity 2015 Waters Corporation 41
42 2015 Waters Corporation 42
43 Mike Wilson 30 th May Waters Corporation 43
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56 What if...
57 2015 Waters Corporation 57
58 What is REIMS? Rapid Evaporative Ionization Mass Spectrometry iknife Hand-held sampling device Diathermy Generator Informatics System Sample REIMS Source 2015 Waters Corporation 58
59 How does it work? 2015 Waters Corporation 59
60 How does it work? 2015 Waters Corporation 60
61 Product Availability 2015 Waters Corporation 61
62 Workflow o Fast, direct ionization o No sample prep required o No chromatography required Acquire Import Analyze Identify Review 2015 Waters Corporation 62
63 Workflow Acquire Import Analyze Identify Review o Easily manage large datasets o Handle multiple replicates o Enhance data quality 2015 Waters Corporation 63
64 Workflow o Easy to use multivariate statistics o Differentiate sample groups o Highlight significant features Acquire Import Analyze Identify Review 2015 Waters Corporation 64
65 Workflow Acquire Import Analyze Identify Review Flexible compound search methods o Compatible with popular databases o Confident assignments o 2015 Waters Corporation 65
66 Workflow Effective visualization tools o Integrate with other informatics o Employ pathway analysis o Acquire Import Analyze Identify Review 2015 Waters Corporation 66
67 Applications Food Research 2015 Waters Corporation 67
68 Applications Food Research 2015 Waters Corporation 68
69 Applications Health Sciences Research 2015 Waters Corporation 69
70 Applications Health Sciences Research The REIMS Research System with iknife Sampling is intended for research use only, and is not intended for diagnostic or therapeutic use 2015 Waters Corporation 70
71 Find out more Waters Hospitality Suite Renaissance Grand Hotel, Ballroom E Mon Wed, 8pm 11pm 2015 Waters Corporation 71
72 Find out more Visit us at the Waters Hospitality Suite Renaissance Grand Hotel, Ballroom E Mon Wed, 8pm 11pm 2015 Waters Corporation 72
73 Find out more Waters Corporation 73
74 2015 Waters Corporation 74
75 Ambient Ionisation Mass Spectrometry Mike Morris Waters Users Meeting ASMS St Louis 30 th May Waters Corporation 75
76 Newborn Screening Inherited metabolic disorders 2015 Waters Corporation 76
77 Acylcarnitine analysis Duke University RCOO H (CH 3 ) 3 N CH 2 CH CH COOH Butylation RCOO H Charlie Roe (CH 3 ) 3 N CH 2 CH CH COOC 4 H 8 H Dave Millington CID - RCOOH -(CH 3 ) 3 N -C 4 H 8 [ CH 2 CH CH COOH ] + (m/z 85) Don Chace 2015 Waters Corporation 77
78 Amino Acids & Acylcarnitines Neutral and acidic amino acids Neutral Loss 102Da 2-minute measurement Up to 50 metabolic disorders Acylcarnitines Precursors of m/z Waters Corporation 78
79 Adoption of MS/MS in the US North Carolina, 1997 New England, 1998 California, 2004 New York, 2005 Texas, 2006 It ll never catch on, you know! 2015 Waters Corporation 79
80 MS/MS in Neonatal Screening Laboratories In the US: All states have Tandem MS programs >80% use Waters instruments In the UK: Leeds, Manchester, Birmingham, Newcastle, Sheffield, North London, South London, Glasgow, Portsmouth, Glasgow In Europe: Belgium, Germany, Netherlands, Sweden, Hungary, Italy, Norway, Switzerland, Russia, UK, Denmark RoW: Australia, Saudi Arabia, Brazil, Argentina, China, Canada, Hong Kong, Korea, Turkey, Kuwait, Taiwan, Japan, Costa Rica, Malaysia, Thailand, Oman, Philippines, Vietnam. In 2015, between 12,000,000 and 15,000,000 babies will be screened using tandem mass spectrometry Waters Corporation 80
81 REIMS and DESI Plenary Speakers Ambient MS in clinical diagnostics from microbiology to MS-guided surgery Professor Zoltan Takats, Imperial College London Addressing complex and critical food integrity issues using the latest analytical technologies Professor Chris Elliott, Queen s University Belfast 2015 Waters Corporation 81
82 [ THANKS ] 2015 Waters Corporation 82
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