Curriculum Vitae. Education: Research Associate (Sept Current)

Transcription

1 Curriculum Vitae Pramod Kumar Giri, Ph.D Research Associate, Department of Microbiology & Immunology, Feinberg School of Medicine, Northwestern University, Chicago, IL USA. Phone: Lab : Cell : pramodgiri75@gmail.com Education: Research Associate (Sept Current) Postdoctoral Research Associate (Sept Oct. 2009) Ph. D (July, 2006): M.Sc. (June, 2000): Department of Microbiology & Immunology, Feinberg School of Medicine, Northwestern University, Chicago, IL USA. Eck Center for Global Health, Department of Biological Sciences, University of Notre Dame, Notre Dame, IN USA. Tuberculosis Immunology Laboratory, Department of Biochemistry, Post Graduate Institute of Medical Education & Research, Chandigarh India. Department of Biotechnology, Indian Institute of Technology (IIT) Roorkee, Uttarakhand India. Ph.D Thesis: Evaluation of new generation Th1 adjuvants for the development of antituberculous mucosal vaccine based on Mycobacterium tuberculosis Ag85 complex proteins. Ph.D Mentor: Professor G.K. Khuller, Senior Professor (former Head of the Department), Department of Biochemistry, PGIMER, Chandigarh, , India.

2 Honors and Awards: 1. Included in Who's Who in America, 2011 Edition. 2. Reviewer for BMC Biotechnology journal. 3. Recipient of Senior Research Fellowship of Indian council of Medical Research (ICMR), Gov. of India, New Delhi [ ]. 4. Recipient of Junior Research Fellowship of Indian council of Medical Research (ICMR), Gov. of India, New Delhi [ ]. 5. Qualified Junior Research Fellowship of Indian council of Medical Research, Gov. of India (ICMR), New Delhi [2002]. 6. Qualified, National Eligibility Test for Junior Research Fellowship of Council of Scientific and Industrial Research (CSIR) Govt. of India, New Delhi. [Dec. 2001]. Research Skills: Proteomics techniques like MALDI-TOF, LC-MS-MS, 1D and 2D-PAGE, whole gel electro-elution, multi-elution, Western blotting, N-terminal sequencing. Protein purification techniques like gel filtration chromatography, ion-exchange chromatography, affinity chromatography, hydrophobic interaction chromatography etc. Handling of pathogenic (M. tuberculosis) and non-pathogenic cultures. Tissue culture techniques- Maintaining mammalian cell line, generation of bone marrow derived macrophages/dendritic cells etc. Immunological techniques- Generation of hybridoma, generation of bone marrow chimera and adaptive transfer in mice, flow cytometery, ELISPOT, cytotoxic T cell assay, ELISA, lymphocyte proliferation assay, estimation of various cytokines, neutrophil activation assay, macrophage activation assay, western immunoblotting, dot blot, raising antibodies, purification of antibodies. Cell biology technique- Con-focal microscopy, Phagosome isolation, microvesicles and nanovesicle (exosomes) isolation etc. Molecular biology techniques DNA & RNA extraction form animal tissue and cells, Q-PCR etc. Microbiology techniques Phagocytosis assay, microbial cultures, colony forming units (CFU) enumeration etc. Handling of experimental animals like-mice and Guinea pigs. Computer skills 1. Flow-cytometry data analysis using Fow-Jo and WIN-MDI software. 2. Statistical software SPSS and Sigma Plot.

3 3. PC applications, MS office, MS DOS, Paint, Adobe Photoshop etc. Publications 1. Choi HJ, Geng Y, Cho H, Li S, Giri PK, Felio K, and Wang CR. Differential requirements for the Ets transcription factor Elf-1 in the development of NKT cells and NK cells. Blood, Dec 2010; doi: /blood Giri PK, Kruh N, Dobos-Elder KM, Schorey JS. Proteomic analysis identifies highly antigenic proteins in exosomes from M. tuberculosis-infected and culture filtrate protein-treated macrophages. Proteomics Sep; 10(17): Giri PK, Verma I and Khuller GK. Adjunct immunotherapy with Ag85 complex proteins based subunit vaccine in a murine model of Mycobacterium tuberculosis infection. Immunotherapy (1): Giri PK. How could we have better vaccines against TB? Expert Opin Biol Ther Nov; 8(11): Giri PK, Schorey JS. Exosomes derived from M. bovis BCG infected macrophages activate antigen-specific CD4+ and CD8+ T cells in vitro and in vivo. PLoS ONE Jun 18; 3(6):e Giri PK & Khuller GK. Is intranasal vaccination a feasible solution for tuberculosis? Expert Rev Vaccines Nov; 7(9): Giri PK, Verma I and Khuller GK. Enhanced immunoprotective potential of Mycobacterium tuberculosis Ag85 complex proteins based vaccine against airway Mycobacterium tuberculosis challenge following intranasal administration. FEMS Immunol Med Microbiol Jul; 47(2): Giri PK, Verma I and Khuller GK. Protective efficacy of intranasal vaccination with Mycobacterium bovis BCG against airway Mycobacterium tuberculosis challenge in mice. Journal of Infection Nov; 53(5): Giri PK, Sable SB, Verma I and Khuller GK. Comparative evaluation of intranasal and subcutaneous route of immunization for development of mucosal vaccine against experimental tuberculosis. FEMS Immunol Med Microbiol Jul 1; 45(1):87-93.

4 Conferences, Symposiums and Professional Training: 1) 2009 (January). Keystone symposia on Tuberculosis: Biology, Pathology and Therapy. Keystone Resort, Keystone, Colorado, USA. Poster Title: Pramod K Giri, Nicole Kruh, Karen M. Dobos-Elder, Jeff Schorey. Proteomics approach to identify the mycobacterial proteins present on exosomes isolated from M. tuberculosis infected macrophages. 2) 2008 (July). 43rd US-Japan tuberculosis & Leprosy Research Conference.. Poster Title: Giri PK, Schorey JS. Exosomes from mycobacterium infected macrophages augment specific T cell immunity. 3) 2008 (May). Oral presentation in Chemistry-Biochemistry-Biology Interface (CBBI) meeting at University of Notre Dame. Giri PK, Schorey JS. Exosomes from M. bovis BCG infected macrophages augment specific CD8+ T cell immunity. 4) 2005 (Feb). Attended International Conference on Functional Genomics for Vaccine & Novel Drug Design against Mycobacteria, at IIT Kharagpur, India [Poster presentation]. 5) 2004 (July). Attended 31 st Annual conference of Indian Immunology Society organized by Department of Biotechnology, Anna Malai University, Chennai, India [Poster presentation]. 6) 2003 (March). Qualified Advanced Course in Research Methodology and Biostatistics at PGIMER, Chandigarh. This 2-month course was aimed at teaching medical professionals and research fellows the intricacies of medical research viz. study designs and protocols, biases, ethical considerations, subject selection, data collection, processing and analysis. I also learned the use of advanced statistical procedures applied in different research trials and studies. 7) 2003 (April). Qualified Advanced Course on the safe use of radioactive materials at PGIMER, Chandigarh, India. I learned the techniques involved in the safe use and disposal of radioactive materials in the hospitals and associated clinical and research laboratories in this 6 weeks course.

5 Professional Referees Chyung-Ru Wang, Ph.D. Professor Department of Microbiology and Immunology, Feinberg School of Medicine, Northwestern University 320 E. Superior Ave, Searle 3-401, Chicago, IL 60611, USA Phone: Prof. G. K. Khuller, Ph.D. Senior Professor (Former Head of the Department) Department of Biochemistry, PGIMER, Chandigarh [India] Tel Res , Cell No Dr. J. S. Schorey, Ph.D. Associate Professor, Department of Biological Sciences, 129 Galvin Life Science Center, Notre Dame, IN 46556, USA Tel Dr. Suraj B. Sable, D.V.M, Ph.D Immunology Activity Leader, Mycobacteriology Laboratory Branch, Division of Tuberculosis Elimination, Bildg. 17, Room No 4042, MS-G35, Centers for Disease Control and Prevention, 1600 Clifton Road, NE, Atlanta, GA, USA Phone: Fax: