1 The Human Genome Project From genome to health From human genome to other genomes and to gene function Structural Genomics initiative June 2000
2 What is the Human Genome Project? U.S. govt. project coordinated by DOE and NIH Initiated in 1990 goals ( ) identify the approximate 100,000 genes in human DNA determine the sequences of the >3 billion bases that make up human DNA store this information in databases develop tools for data analysis address the ethical, legal, and social issues that arise from genome research
3 The Human Genome Project Project began in 1990 as a $3 billion, 15-year effort 18 countries participate in the worldwide effort About 3 yrs ago -A private company - Celera announced that the human genome will be completed within 3 years. The human genome was immediately associated with additional sequencing projects - microbial, pathogen, plants, Fugu fish, mouse etc.
5 The Human Genome Project September 1999 public announcement: A rough draft by spring 2000 The draft About 90% of the human genome A complete, high-quality DNA sequence - by 2003 Generating the draft will require to sequence each piece of DNA only about 5 times, instead of the usual 10 times used for obtaining the highest quality sequence (99.99%).
7 DNA sequencing
8 The Human Genome Project From the news: The Human Genome Project international consortium announced (April 2000) that 2 billion of the 3 billion letters that constitute the genetic instruction book of humans have been deciphered and deposited into GenBank. GenBank, the public database of DNA sequence operated by the National Institutes of Health, is accessible freely and without restrictions to all scientists in industry and academia.
9 The Human Genome Project Some numbers: Human Genome Project assembles 12,000 bases every minute. 15 billion raw base pairs were sequenced to reach the two billion. Each area of a chromosome at least four to five times to insure that the data deposited is accurate. The depth of coverage,
10 Cancer genome projects and the search for disease genes One of the challenges of the post-genomic era is to establish the role of genes in common multifactorial disease. Cancer in the western world About one in three people will get cancer over their lifetime, and about one in five will die of it. The goal - discovery of the disease genes
11 Cancer genome projects During life, human cells acquire changes to their DNA as a result of : Exposure to the environment - chemicals, radiation or viruses Errors in the enzymatic machinery that copies DNA during cell division
12 Cancer genome projects Most of these changes are put right by the cell's repair mechanisms. If mistakes occur in genes known as oncogenes or cancer genes, the cell in which those changes occur can start to behave abnormally. The cell may continue dividing when it should be stopping
13 Cancer genome projects Following isolating the genes... Which genes are abnormal in which cancers Which cancer have many abnormalities and which have few abnormalities In Sanger center (UK) screen 2000 cancers of all types - childhood, adult, epithelial and soft tissue
15 The growth in sequencing - last decade
16 Chromosomes -over the globe
17 Chromosomes content Chromosome million bases, 6 % of the genome. Disease-linked genes: colorectal cancer, basal cell carcinoma, acute myelogenous leukemia, salt-resistant hypertension type of dwarfism. Chromosome million 3 % of the genome. breast and prostate cancer, Crohn's disease adult polycystic kidney disease (5 million people worldwide. 50% require dialysis or kidney transplant). Chromosome million, 2% of the genome. DNA damage atherosclerosis diabetes mellitus
18 Chromosomes 22 landscape Chromosome 22 - the longest sequenced piece (up to Chr.21) 545 genes, 134 pseudogenes and 200 to 300 additional ones. Size of a gene - 1,000 to 583,000 bases of DNA. 39% of the chromosome is copied into RNA (exons and introns). 247 genes were revealed to be identical to previously identified genes. There are families of genes that are distributed over large chromosomal regions. Unexpected long-range complexity of the chromosome with an elaborate array of repeat sequences near the centromere. several regions where recombination is increased, and others where it is suppressed.
19 Human chromosomes Trisomy of chromosome 21 Down syndrome Nature, 18th May 2000
20 The latest -18 May 2000
22 Latest news: Genetic information on human chromosomes 5, 16 and 19. The chromosomes contain an estimated 10-15,000 genes. Genetically linked diseases : kidney disease, prostate and colorectal cancer, leukemia, hypertension, diabetes and atherosclerosis. 300 million base pairs, or an estimated 11 percent of the total human genome
23 Cancer genome projects How to isolate those genes? About 100 cancer genes have been found Most of the 100 genes have been found in the rare leukaemias and lymphomas, which account for less than 10 % of all human cancer For the common adult cancers of the breast, colon, prostate, lung and ovary, which account for 80 % of the cancer burden, only about 30 genes are known.
24 Cancer genome projects Future medicine... The genes are likely to be the key targets for new types of therapy. For example: If a gene is mutated so it is permanently active, a therapy could inactivate the protein. If a gene has been turned off by the mutation, perhaps it could be turned back on or a second, normal copy introduced.
25 Screening genome projects Future medicine... similar kinds of screen can be performed for other human diseases that we know have a genetic component - for example diabetes and heart disease. Long term goal: Asthma, Dyslexia, Autism..
26 Benefits of HGP Research Medical Benefits improved diagnosis of disease earlier detection of predispositions to disease rational drug design gene therapy and control systems for drugs pharmacogenomics personal drugs organ replacement
27 Benefits of HGP Research Microbial Genome Research new energy sources (biofuels) environmental monitoring to detect pollutants protection from biological and chemical warfare safe, efficient toxic waste cleanup
28 Benefits of HGP Research DNA Forensics identify potential suspects at crime scenes exonerate wrongly accused persons identify crime and catastrophe victims establish paternity and other family relations identify endangered and protected species as an aid to wildlife officials (prosecution of poachers)
29 Benefits of HGP Research DNA Forensics - cont. detect bacteria and other organisms that may pollute air, water, soil, and food match organ donors with recipients in transplant programs determine pedigree for seed or livestock breeds authenicate consumables such as caviar and wine
30 Benefits of HGP Research Agriculture and Livestock disease-, insect-, and drought-resistant crops healthier, more productive, disease-resistant farm animals more nutritious produce biopesticides edible vaccines incorporated into food products new enviornmental cleanup uses for plants like tobacco
31 Benefits of HGP Research Evolution and Human Migration use germline mutations in lineages to study evolution study migration of different population groups based on female genetic inheritance study mutations on the evolutionarily stable Y chromosome to trace lineage and migration compare breakpoints in the evolution of mutations with ages of populations and historical events
32 Benefits of HGP Research Risk Assessment assess health damage and risks caused by radiation exposure, including low-dose exposures assess health damage and risks caused by exposure to mutagenic chemicals and cancer-causing toxins reduce the likelihood of heritable mutations
33 Future medicine 99.9 per cent of our DNA sequence is identical to that of all other humans One nucleotide base in every 1000 of the 3 billion total, differs from one individual to another. A simple blood sample could provide a complete genetic profile that predicts our susceptibility to disease, our response to drugs, our propensity to obesity or anxiety.
34 Differernces among individuals Each of us has a unique combination of polymorphisms (SNPs). This polymorphism underlies the fact that some have blue eyes and some brown, some are tall and some short - and... some suffer heart attacks in middle age while others are very healthy at >90 years of age.
35 The role of Bioinformatics in the Human Genome Project
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