SUPPLEMENTARY DATA. PRIBA Study Group. Lead Centre:



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PRIBA Study Group Lead Centre: Royal Devon and Exeter NHS Foundation Trust/University of Exeter: Anita Hill, Rob Bolt, Jane Stewart, Bridget Knight, Tim McDonald, Beverley Shields, Angus Jones, Andrew Hattersley, Gayle Githens-Mazer, Tina Sanders, Kirsty Wensley NIHR Clinical Research Network: Ipswich Hospital NHS Trust: Gerry Rayman, Sue Hood, Jo Rosier, Jane Jiao, Debbie Simmonds, Caroline Calver North Bristol Hospitals NHS Trust: Andrew Johnson, Sharon Tovey, Jade Bennet, Dafydd Wilson Evans, Philippa Lamb, Hilary Holloway, B Moore Northampton General Hospital NHS Trust: Charles Fox, Kathy Hall, L James, C Smith Northern Devon Healthcare NHS Trust: Alastair Watt, Geraldine Belcher, Amanda Skinner Oxford Centre for, Endocrinology and Metabolism: Steve Gough, Judy MacDonald, Lynne Nairn, Sue Rous Plymouth Hospitals NHS Trust: Ann Millward, Margaret Blackmore, Migaila Watt Portsmouth Hospitals NHS Trust: Mike Cummings, Sharon Allard, Elaine Hallett, Jane Rowney Royal Bournemouth and Christchurch Hospitals NHS Foundation Trust: David Kerr, Patricia Sanders, Carina Vickers Royal Cornwall Hospitals NHS Foundation Trust: Steve Creely, Duncan Browne, Helen Chenoweth, Terri Chant, Sue Durkin Royal Stoke University Hospital North Midlands: Ellen Hodgson, Gemma Reddell, Loretta Barnett, Jane Deleaney South Devon Healthcare NHS Foundation Trust: Richard Paisey, Sue Bunce, Dawn Tomlinson, Mary Costello South Warwickshire NHS Foundation Trust: Peter Horrocks, Penny Parsons, Alex Smith Surrey and Sussex Healthcare NHS Trust: James Clark, Tracey Shewan, Louise Nimako Taunton and Somerset NHS Foundation Trust: Rob Andrews, Catherine Thompson, Donna Archer West Hertfordshire Hospitals NHS Trust: Thomas Galliford, Elaine Walker, Lynn Curry, Sindi Masuka, Cathy Constantin Yeovil District Hospital NHS Foundation Trust: Seshadri Pramodh, Linda Balian, James Gibbons, Claire Buckley

Supplementary Table 1. The relationship between baseline markers of beta cell function and HbA1c change after GLP-1RA therapy in islet autoantibody negative participants (N=501). *A negative regression coefficient denotes a greater HbA1c reduction with a higher baseline value or presence of dichotomous state Insulin cotreatment 0.23 0.025 1.79-2.6 5-0.41 0.08 Supplementary Table 2. The association between insulin co-treatment and markers of beta cell failure. positive Insulin treated Mean (95% CI) or % positive Non insulin treated Mean (95% CI) or % positive 12.9 (11.9, 13.9) 1.4 (1.3, 1.5) 4.3 (4.0, 4.7) P value (T test or Chi 2 ) 8.1 (7.6, 8.7) (0.85, 1.0) 2.4 (2.1, 2.8) 8% %

Supplementary Table 3. The relationship between baseline markers of beta cell failure and HbA1c change after incretin therapy within insulin treated participants (n=209). *A negative regression coefficient denotes a greater HbA1c reduction with a higher baseline value or presence of dichotomous state. positive** 0.18 0.2 (-0.1, 0.4) -4.3 (-7.6, -1.0) -1.1 (-1.9, -0.2) 8.1 (0.7, 15.5) 0.03 Supplementary Table 4. The relationship between baseline markers of beta cell failure and HbA1c change after incretin therapy within non-insulin treated participants (n=337). *A negative regression coefficient denotes a greater HbA1c reduction with a higher baseline characteristic value or presence of dichotomous state. **only 3 non insulin treated participants were antibody positive positive** *Regression coefficient 0.06 (-0.24, 0.36) -0.1 (-2.9, 2.7) 0.02 (-.51, 0.54) -1.6 (-18.7, 15.6) HOMA2%B -.04 (-0.10, 0.02) Significance (p) 0.7 0.16

Supplementary Table 5. The relationship between baseline markers of insulin resistance and HbA1c change after incretin therapy. *A negative regression coefficient denotes a greater HbA1c reduction with a higher baseline characteristic value. ** non insulin treated participants only BMI (Kg/m 2 ) Triglycerides (mmol/l) SHBG HDL (mmol/l) HOMA2%S** (-0.04 0.07) - (-0.22, 0.16) 7 (-0.46, 0.47) - 0.7 (-0.09, 0.06) -0.67 0.8 (-5.4, 4.1) 0.67

Supplementary Figure 1. Scatterplot showing the relationship between baseline fasting C- and HbA1c change (6 months minus baseline, adjusted for co-treatment change and baseline HbA1c) after GLP-1 receptor agonist therapy. Linear regression B = - 3.2, p=0.002-100 -50 0 50 0 1 2 3 4 (nmol/l) HbA1c change Fitted values