Mar. 2010, Volume 4, No.2 (Serial No.27) Journal of Life Sciences, ISSN 1934-7391, USA 200090 幷 1. 幷 [1] [2] [3] [4] Scylla spp. Crustacea Decapoda Brachyura Portunidae 1. Genetic diversity * 2006BAD03B08-09 2007Z01 2008M02 1979 E-mail: kjjiang@hotmail.com. 1972 E-mail: malingbo@vip.sina.com. 45
Molecular marker DNA Genetic marker DNA [5] DNA [6] 3. 9 [7] 1 2 3 4 5 6 7 8 9 Restriction Fragment Length Polymorphism RFLP [8,9] DNA Random Amplified Polymorphic DNA RAPD [10] Amplified Fragment Length Polymorphism AFLP [11] DNA Simple Sequence Repeat SSR [12-14] Inter-Simple Sequence Repeats ISSR [15] DNA Single-Nucleotide Polymorphism SNP [16] DNA Mitochondrial DNA mtdna [17] Expressed sequence Tag, EST [18] Sing1e Strand Conformation Po1ymorphism SSCP DNA DNA Amplification Fingerprinting DAF Denaturing Gradient Gel Electrophoresis DGGE Temperature Gradient Gel Electrophoresis TGGE 4. DNA S. serrata [19] S. serrata, S. oceanic, S. tranquebarica [20] S. serrata, S. tranquebarica, S. paramamosain, S. olivacea [21] DNA 幷 DNA DNA DNA 5 72 72 COI [22] 12 46
COI 98% 9.98% 10.84% 0.00783 0.11659 [23,24] DNA Gopurenko 3 N=21 3 N=76 5 N=28 S. serrata [25] I I II 38 Numts [26] COI [27] 5 [28] 10 [29] 5. 1997 2003 180 5.1 [30] [31,32] [33] 1 [34] 2 3 4 DNA PCR 5.2 47
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