HiPic High Performance image control and Processing software
testimonials about HiPic, u8913 HiPic is Hamamatsu s sophisticated software for physical and industrial applications. It s not only Hamamatsu monochrome DCAM based cameras such as scmos ORCA-Flash series or ImagEM X2 series that are supported. Line sensors or X-ray cameras can be used with HiPic too. In the newest version image intensifiers and mini-spectrometers are integrated now as well. HiPic is easy to use and along with full control of all camera features, it offers various analysis functions such as sophisticated correction algorithms, calibration and arithmetical operations. Features Full support of all Hamamatsu monochrome cameras Full support of all X-ray Flat panels, X-ray Line sensors, X-ray TDI sensors and MFX tubes Extended range of cameras and frame grabbers by internal driver modules (e.g. analog video cameras and TDI cameras) Support of mini-spectrometers Support of image intensifiers Control of all camera features Long term support of legacy hardware, maximum lifetime guarantee Support of freely definable camera through GenericCam module Sequence acquisition at high frame rate Set of triggering functions for perfect synchronization with external devices applications X-ray imaging Spectroscopy Fluorescence and Luminescence imaging Photon counting Semiconductor imaging Scintillator readout High speed imaging 2
image acquisition and camera control All features of any Hamamatsu camera can be fully controlled by HiPic. Besides image acquisition, these include related features like temperature control of the CCD chip, overexposure security functions (ImagEM X2 series) and DSP functions. For the acquisition of the perfect image, HiPic offers various acquisition modes: live mode: A continuous series of images for adjustments and optical alignment. acquire mode: Records individual images to keep the image data for subsequent analysis. analog integration mode: Memory integration up to 32 bit / pixel to reduce noise for high precision measurements. All acquisition modes allow for variable bit depth (8, 10, 12 14 or 16 bit), feature binning (standard and multi-line, depending on camera type) and sub-array functionality. Image data can be saved in a proprietary format or in 8 or 16 bit TIFF format. If the camera is to be used with other hardware or should be synchronized to external events, various trigger modes allow different timing modes for image acquisition. sequence mode: Acquires a series of single images to RAM or hard disk, quickly and effi ciently. Images of a sequence can be accessed and analyzed individually or as batch. streaming mode: Saves data continuously to hard disk into a single streaming fi le. It allows acquiring images over a long time, limiting the number of acquired frames to the available hard disk space. additional acquisition modes are available: Photon counting mode: Dynamic photon counting (in combination with image intensifi er) and offl ine photon counting analysis. PiV mode: PIV (Particle Image Velocimetry) mode enables quick interval image acquisition. Images are saved as pairs. 3
selected Publications image display and analysis HiPic offers various possibilities for displaying and analyzing recorded images. In addition to the image itself, rulers and multiple profi les can be displayed in combination with the image or separately. Furthermore images can be superimposed, scaled, and shown in linear or logarithmic coordinates. A zoom allows for viewing sub regions of the image or the profi le. In case of line sensors or TDI cameras, HiPic offers a scrolling LIVE display. For image analysis multiple Rois can be defined, either area or line with optional display of the respective profiles inside or outside the image area. The look up table (LUT) is used to adapt the dynamic range of the image data for display and allows visualizing even very faint images clearly. Black and white or pseudo colors can be used for display. In very critical cases a nonlinear LUT can be applied. HiPic offers the following analysis functions: Multiple regions of interest (ROIs) with optional display of profi les. Sophisticated profi le tool allows for sampling data along a user defi ned sampling line. Intensity profi le analysis can handle up to 10 profi les simultaneously. Analytical parameters such as the peak position, FWHM, fall or rise time can be easily obtained on the fl y Distance / area measurements Histogram analysis Arithmetic operations on one image or between images For further analysis, image data and profi le data can be saved to ASCII fi les and imported into other programs like Excel, Origin or MATLAB. 4
image correction and calibration For extracting sensitive data from an image, image correction and calibration is crucial. HiPic supports image data correction functions required for scmos cameras, CCD cameras and video imaging, including: Background subtraction for correction of an offset signal caused by CCD dark current, non-uniform video noise or background light such as stray light or auto fl uorescence signal. Shading correction (fl at fi eld correction) for compensating overall system non-uniformity caused by imperfect illumination or imperfect optical systems (vignetting). Defect pixel correction for correction of defective pixels, lines or columns (hot pixel / dead pixel). Apart from image correction, another crucial tool is image calibration. Often it is more interesting to see the length, time or wavelength coordinates instead of plain pixel axis. Consequently HiPic offers the possibility to calibrate the camera display: physical units are assigned to the respective pixel coordinates. Both spatial axes can be independently calibrated using linear or nonlinear functions and arbitrary units. The calibration information is attached to all measurement data like images or profi les. All corrections can be performed real-time, automatically after the measurement or offline as a separate step. Some cameras allow for real-time correction functions directly in their fi rmware or in a DSP this feature is of course supported in the software as well. For reducing noise while keeping the dynamic aspect of LIVE mode, recursive fi lters can be applied. 5
Remote control and scripts Almost all software functions like image acquisition or image data save can be controlled from other applications by the RemoteEx tool. RemoteEx is based on simple text commands sent via TCP-IP across a network (LAN) or from an application executed on the same computer. Image data can be sent by TCP-IP or exchanged by saving the data to a file and loading it from the other application. RemoteEx allows easy integration of functionality from almost every other application, across a network and also from other platforms like UNIX. RemoteEx is included in the standard distribution and can be used freely by everybody. It also includes a script functionality which allows building of automated measuring sequences. Sample scripts are also part of the distribution. distribution / download / license client program Downloadable through the internet and can be freely distributed, dongle protected. Possibility of checking, troubleshooting and image processing using demo mode without dongle. control by tcp-ip HiPic data and events 6
application examples HiPic can be used to acquire and process images with an X-ray source and detector. One application example is the investigation of mechanical condition of product and wrapping in the pharmaceutical industry. The image was taken with a L9181-02 microfocus X-ray source at 130 kv and a C7942SK-25 flat panel detector. X-ray image of a C-mount surveillance lens with plastic fitting. The image was taken with a Hamamatsu L9181-02 microfocus X-ray source at 130 kv and a C7942SK-25 flat panel detector. mini-spectrometer HiPic provides the functionality to grab spectra from USB mini-spectrometers. Besides acquiring a spectrum profile, analysis offers the possibility to review peak positions, FWHM of the bands, integral and more. Photon counting using image intensifiers For very low light applications using image intensifiers HiPic offers the special photon counting mode. If the used image intensifier provides enough gain to register single photon counting events, they are added up in the frame memory. In this way, very high signal-to-noise ratios can be achieved, when signal integration times are sufficiently long. This mode also effectively suppresses certain crosstalk effects inherent to some image intensifiers. A dynamic photon counting mode allows temporal analysis of photon counting images. 7
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