Giuseppe Torzillo. Italian Viggiano (Potenza)



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C U R R I C U L U M V I T A E F O R M A T O E U R O P E O / E U R O P E A N F O R M A T INFORMAZIONI PERSONALI/ PERSONAL INFORMATION Nome, Cognome/Name, Surname Indirizzo/Address Via, numero civico, c.a.p., città, nazione/ House number, street name, postcode, city, country Giuseppe Torzillo Telefono/Telephone + 39.055.522.5992 Via B. Buozzi, 110, 50013, Campi Bisenzio, Firenze, Italy Fax + 39.055.522.5920 E-mail Sito web/website Nazionalità/Nationality Luogo e data di nascita/ Place and Date of birth torzillo@ise.cnr.it www.cnr.it Italian Viggiano (Potenza) ESPERIENZA PROFESSIONALE /WORK EXPERIENCE Se dipendente CNR indicare: N. MATRICOLA. 20382 QUALIFICA: SENIOR RESEARCHER LIVELLO: SECOND In ordine di data /Dates (from 2010 to 1994) [Iniziare con le più recenti ed elencare separatamente ciascun incarico ricoperto/ Add separate entries for each relevant post occupied, starting with the most recent. ] Page 1 - Curriculum vitae 1) 30- years of research with the National Research Council of Italy (CNR); 2) 2011 Head of the Section of Florence of the Institute of Ecosystem Study. 3) Since 1995-Project leader of Bilateral CNR/AVCR project (Czech Republic); 4) (2006-2009)- Responsible of the research line Develompment of a high-performance photobioreactor design for hydrogen production Project Hydrobio (http://www.idrobio.net), supported by the Italian Ministry of Education (MIUR); 5) 2007-2009: Local coordinator within the project EBH2: Electro- Bio-Idrogeno, supported by Tuscany Region (Italy); 6) Since 2009: Italian representative within the International Energy Association- Hydrogen Implementing Agreement, (IEA-HIA) - Task 21 Bio-Hydrogen; 7) 2006-2009. Local responsible in the European Coordinated Action (Aquagris) Environmental management reform for

sustainable farming, fisheries and aquaculture; 8) 2008. Member of the International Scientific Committee of the First International Congress on Biotechnology and Bioengineering November 5-7, 2008, Mexico City. 9) 2008 - Member of the Scientific Committee of the Conference Combustibili da biomasse: aspetti innovative. Organized by AIDC Associazione italiana di ingegneria chimica, Facoltà di Ingegneria dell Università La Sapienza Roma. 26 marzo 2009; 10) 2007-Member of the International Symposium on Applied Phycology and Environmental Biotechnology (ISAPEB-2007), Pilani (India); 11) 2006-Member of the scientific committee of the Forth European Phycological Congress, Oviedo (Spain); 12) Since 2006- Member of the Italian Society of Botany 13) 2005- Member of the VII Congreso de Ficología de Latinoamérica y el Caribe y Reunión Iberoamericana de Ficología. Cuba, 19-24 September, 2005. 14) 2003- Local responsible in the European Thematic network "Alginet: microalgae as cell factories for chemical and biochemical products"; 15) 2003- Scientific expert for the Italian Ministry of Education (Albo Esperti MIUR); 16) Since 2003 -Member of the Executive Committee of the International Society for Applied Phycology (ISAP); 17) Since 1999- local coordinator of the research project Effect of ionizing radiations on the oxygen-evolving activity of photosynthetic microorganisms sponsored by ASI (Italian Space Agency); 18)1997-1998: Project leader (Italian side) of a joint research between CNR (Italy) and MOSE (Ministry of Science and Technology, Israel) on chlorophyll fluorescence; 19) 1999- Member of the Scientific Committee of the VIII International Conference of the Society of Applied Algology, held in Italy; 20) 1994- Member of the Scientific Committee of the VIII Symposium on Phototrophic Prokaryotes held in Italy; 21) Since 1995, Project leader (Italian side) of a joint research between CNR and Department of Autotrophic Microorganisms, Institute of Microbiology, Czech Academy of Sciences; Teaching activity in training course 2011- Biotechnology of cultivation of microalgae and cianobacteria and their applications to the production of clean energy and environmental protection. University of CR 12-16 December, San Josè, Costa Rica. 2010 - Advanced Training Course in Algal Biotechnology (Izmir 25-29 October 2010), Turkey. 2009 - Advanced training course in algal biotechnology. King Mongkut s University of Technology Thonburi. September 28 th - October 2 nd, Bangkok, Thailand. 2007- Photosynthesis and chlorophyll fluorescence measurements to optimize growth of outdoor cultures of microalgae. University of Costa Page 2 - Curriculum vitae

Rica Escuela de Biologia. November 24 th December 5 th, San Josè, Costa Rica. 2006- Preconference workshop: Measurements of Algal growth and photosynthesis October 9-11. Institute of Biological Sciences College Laguna Philippines. 2006- European Summer School Production of fluels, specialty chemicals and biobased products from agro-industrial wastes and surplus. Faculty of Engineering. University of Bologna (Italy). 2006 - Photosynthesis physiology in microalgae and higher plants. Regular course at the University of Costa Rica Escuela de Biologia. July 31- August 11. 2003- International Workshop and Training Course on Photobioreactors. Ege University, Faculty of Engineering, Bioengineering Department, TUBITAK. Ismir (Turkey) 2-4 April 2003. 2004- Applied Course on Production and Monitoring of Microalgal Growth. Ege University, Faculty of Engineering, Bioengineering Department, TUBITAK. Ismir (Turkey), 7-11 June, 2004; 2004- Regional graduate workshop on microalgal biotechnology (November 29- December 7) UNA-UCR Costa Rica. Nome e indirizzo del datore di lavoro / Name and address of employer Tipo o settore di attività / Type of business or sector Funzione o posto occupato / Occupation or position held Principali mansion e responsabilità / Main activities and responsibilities Consiglio Nazionale delle Ricerche, Piazzale Aldo Moro, 8 Growth physiology and biotechnology of mass culture of photosynthetic microorganisms. He has published more than 80 papers and 8 chapters dealing with photosynthetic microorganisms and patented two photobioreactor designs for outdoor culture of microalgae. Iinvited speaker in several International Conferences dealing with algal Physiology and Biotechnology. Senior Researcher. Head of the Unit of Florence of ISE 1) 2011- Responsible of the research line: Biology and technology of photosynthetic microorganisms (TA P04.034) of the Department Earth and Environment of CNR 2) 2005-2010: Responsible of a research module (T&A) Ecotecnologie di mitigazione dell impatto umano in ambienti lacustri e tecnologie per la depurazione di acque da nutrienti e sostanze organiche con produzione massiva di biomsse algali. 3) 2006-2010-Responsible of research module, within Dept AG: Cultivation of photosynthetic microorganisms. 4) 2003-2008- Member of the Committee of the Istituto per lo Studio degli Ecosistemi, CNR, Italy; 5) 1989-1998-Member of the Scientific Committee of the Centro di Studio dei Microorganismi Autotrofi, CNR; ISTRUZIONE E FORMAZIONE / EDUCATION AND TRAINING Nome e tipo d istituto di istruzione o formazione / Name and type of organisation providing education and training Principali materie e competenze Page 3 - Curriculum vitae -University of Florence -Centro di Studio dei Microorganismi Autotrofi; -Microalgal Biotechnology Laboratory Sede Boker, Israel -National Renewable Energy Lab ( Golden, Colorado) USA Algal Physiology and Biotechnology

professionali apprese / Principal subjects occupational skills covered Certificato o diploma ottenuto /Title of qualification awarded Livello nella classificazione nazionale o internazionale / Level in National classification 1976: Degree in Agriculture with first-class honours from the University of Florence, 1978. Laurea in Scienze Agrarie (Agriculture) Page 4 - Curriculum vitae

ATTIVITA DI RICERCA / RESEARCH ACTIVITIES Attuali campi di ricerca / Research sectors Recenti attività scientifiche/ Recent Scientific Activities. Pubblicazioni/ Books and Articles Algal Physiology and Biotechnology; Bioenergy from microalgae (Biohydrogen and Biodiesel) List of recent papers published by Dr. Giuseppe Torzillo 1) Masojidek J., Sergejevova M., Rottnerova K., Jirka V., Koreccko J., Kopecky J., Zat kova I., Torzillo G., Stys D. (2009). A two-stage solar photobioreactor for cultivation of microalgae based on solar concentrators. J Appl Phycol, 21: 55-63. 2) Torzillo G., Scoma A., Faraloni C., Ena A., Johanningmeier U. (2009). Increased hydrogen photoproduction by means of a sulfurdeprived Chlamydomonas reinhardtii D1 protein mutant. Int J Hydrogen Energy: 34: 4529-4536. 3) Giannelli L., Scoma A., Torzillo G. (2009). Interplay between light intensity, chlorophyll concentration and culture mixing on the hydrogen production in sulfur-deprived C. reinhardtii cultures grown in laboratory photobioreactors. Biotechnol Bioeng 104: 76-90. 4) Faraloni C., Torzillo G. (2010) Phenotypic characterization and hydrogen production in Chlamydomonas reinhardtii Q B binding D1 protein mutants under sulphur starvation: changes in chlorophyll fluorescence and pigment composition. J Phycol, 46: 788-799. 5) Masojidek J., Kopecky J., Giannelli L., Torzillo G. (2011) Productivity correlated to photochemical performance of Chlorella mass cultures grown outdoors in thin-layer cascades. J Ind Microbiol Biotechnol 38: 307-317. 6) Torzillo G. Giannelli L., Martinez-Roldan A.J., Verdone N., De Filippis P., Scarsella M., Bravi M. (2010). Microalgal culturing in thinlayer photobioreactors. Chem Eng Trans, 20: 265-270. 7) Faraloni C., Cutino L., Petruccelli R., Leva AR., Lazzeri S., Torzillo G. (2011). Chlorophyll fluorescence technique as a rapid tool for in vitro screening of olive cultivars (Olea europea L.) tolerant to drought stress. Env. Exper. Botany, 73:49-56. 8) Torri C., Samorì C., Adamiano A., Fabbri D., Faraloni C., Torzillo G. (2011). Preliminary investigation on the production of fuels and biochar from Chlamydomonas reinhardtii biomass residue after biohydrogen production. Biotechnol. Biores, 102: 8707-8713 9) Faraloni C., Ena A., Pintucci C., Torzillo G. (2011). Enhanced hydrogen production by means of sulfur-deprived Chlamydomonas reinhardtii cultures grown in pre-treated olive mill wastewater. Int. J. Hydrogen Energy 36: 5920-5931. 10) Silva M., Torzillo G. (2012). Nitrogen and phosphorus removal through laboratory batch cultures of Chlorella vulgaris and cyanobacterium Plankthotrix isothrix grown as monoalgal and as cocultures. J. Appl. Phycol. 24: 267-276 11) Scoma A., Giannelli L Faraloni C., Torzillo G. (2012). Outdoor H 2 production in a 50-liter tubular photobioreactor by means of a sulfurdeprived culture of the microalga Chlamydomonas reinhardtii. J. Biotechnol. 157::620-627 12) Scoma A., Giannelli L Faraloni C., Torzillo G. (2012) Sustained H 2 production in a Chlamydomonas. reinhardii D1 protein mutant. J. Biotechnol. 157: 613-619 Page 5 - Curriculum vitae

13) Scarsella M., Torzillo G., Cicci A., Belotti G., De Filippis P., Bravi M. (2012). Mechanical stress tolerance of two microalgae. Process Biochemistry (in press). Doi: 10.1016/j.procbio.2011.07.002 14) Samori C., Torri C., Fabbri D., Falini G., Faraloni C., Galletti P., Spera S., Taglisavini E., Torzillo G. (2012). Unusual catalysts from molasses: synthesis, properties and application in obtaining of biofuels from algae. ChemSusChem 5: 1501-1512. 15) Adessi A., Torzillo G. Baccetti E., De Philippis R. (2012). Sustained outdoor H2 production with Rhodopseudomonas palustris cultures in a 50 L tubular photobioreactor. Int. J. Hydrogen Energy, 37: 8840-8849. 16) Torzillo G., Faraloni C., Silva A.M., Kopecky J., Pilny J., Masojidek J. (2012). Photoacclimation of Phaeodactlylum tricornutum (Bacillariophyceae) cultures grown outdoors in photobioreactors and open ponds. Eur J Phycol. 47: 169-181. 17) Cuzman1 O.A., Faraloni C., Pinna D., Riminesi C., Sacchi B., Tiano P., Torzillo G. (2012). Evaluation of treatments efficiency against lichens growing on monumental stones by electrical conductivity (in press). 18) Giannelli L., Torzillo G. (2012). Hydrogen production with the microalga Chlamydomonas reinhardtii grown in a compact tubular photobioreactor immersed in a scattering nanoparticle suspension. Int. J. Hydrogen Energy Doi: http://dx.doi.org./10.1016/j.ijhydene.2012.08.103. Chapter in books Page 6 - Curriculum vitae 1) Torzillo G. (1997). Tubular Bioreactors. In: Spirulina platensis (Arthrospira): Physiology Cell-Biology and Biotechnology. (Edited by A. Vonshak), Taylor and Francis Ltd, London. 2) Torzillo G. and Vonshak A. (2003). Biotechnology for algal mass cultivation. In: "Recent Advances in Marine Biotechnology. (Edited by M Fingerman & R Nagabhushanam). Science Publishers of Enfield, New Hampshire, USA. Vol. 9. 3) Vonshak A., Torzillo G. (2003). Microalgal physiology of environmental stress responses. In: Handbook of Microalgal Cultures (Richmond A. Ed.). Blackwell Science Ltd. Oxford. 4) Masojidek J., Koblizek M., Torzillo G. (2003). Photosynthesis in Microalgae. In : Handbook of Microalgal Cultures (Richmond A. Ed.). Blackwell Science Ltd. Oxford 5) Esposito D., Faraloni C., Fasolo F., Margonelli A., Torzillo G., Zanini A., Giardi MT. (2004) Automatic photosystem II-based biosensors to reveal the effect of complex space radiation on photosynthetic oxygen microorganisms for survival in flight. In: Biotechnological Applications of Photosynthetic Proteins: Biochips, Biosensors, and Biodevices. MT. Giardi and EV. Piletska (Eds). BioScence Publishers, London. 6) Masojidek J., Torzillo G. (2008). Mass cultivation of freshwater microalgae. Ecological Engineering. Encyclopedia of Ecology. Elsevier (eds. Sven Erik Jorgensen and Brian D. Fath). Vol. 3. pp. 2226.2235 7) Sili C., Torzillo G., Vonshak A. (2010). Arthrospira. In: Ecology of cyanobacteria. Whitton BA, Pott M (eds), Kluwer Academia Publishers. Dordrect/ London/ Boston (in press) 8) Masojidek J., Vonshak A., Torzillo G. (2011) Chlorophyll

fluorescence applications in microalgal mass cultures. In: Chlorophyll a Fluorescence in Aquatic Sciences: Methods and Applications. Developments in Applied Phycology 4, D Suggett et al. (eds) pp 277-292 DOI 10.1007/978-90-481-9268-7-13. Sprinter Science + Business Media B.V. 2011. 9) Torzillo G., Seibert M. Hydrogen production by Chlamydomonas reinhardtii (2012) Handbook of Microalgal Cultures (Richmond A., Hu Q. Eds.). Blackwell Science Ltd. Oxford. 10) Bravi M., Cicci A., Torzillo G. (2012). Quality preservation and cost effectiveness in the extraction of nutraceutically-relevant fractions from microbial and vegetal matrices. Scientific, Health and Social Aspects of the FOOD Industry (Valdez B. ed.) Vol. 46, 2012: 463-488. ISBN: 978-953-307-916-5. C) Patents 1) Torzillo G., Carlozzi P., Materassi R. Fotobioreattore con circolazione della massa colturale in un condotto sostanzialmente elicoidale 05.05.1995 N. FI95A 92. 2) Carlozzi P., Torzillo G., Materassi R. Fotobioreattore con circolazione della massa colturale in condotti offrenti curvature successive e sostanzialmente inverse. Titolare CNR a Roma: Data di deposito 05.05.1995 N. FI95A 93. 3) Ena A., Pushparaj B., Carlozzi P., Sacchi A., Torzillo G., Peccianti F., Citernesi S., Pintucci C., Petrai I. Processo per la depurazione e valorizzazione delle acque di vegetazione olearie. Titolare CNR a Roma. Data di deposito 19/06/2006. FI2006A000155. ULTERIORI INFORMAZIONI / ADDITIONAL INFORMATION TRATTAMENTO DEI DATI PERSONALI, INFORMATIVA E CONSENSO Il D.Lgs. 30/6/2003, n. 196 Codice in materia di protezione dei dati personali regola il trattamento dei dati personali, con particolare riferimento alla riservatezza, all identità personale e al diritto di protezione dei dati personali; l interessato deve essere previamente informato del trattamento. La norma in considerazione intende come trattamento qualunque operazione o complesso di operazioni concernenti la raccolta, la registrazione, l organizzazione, la conservazione, la consultazione, l elaborazione, la modifica, la selezione, l estrazione, il raffronto, l utilizzo, l interconnessione, il blocco, la comunicazione, la diffusione, la cancellazione e la distruzione di dati, anche se non registrati in una banca dati. In relazione a quanto riportato, autorizzo il CNR al trattamento dei dati contenuti nel presente curriculum vitae e nella documentazione della quale fa parte integrante ( barrare la casella) X Si, acconsento Page 7 - Curriculum vitae