Workshop Rapid NGS for Public Health Microbiology



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Medical Microbial Genomics Centre Workshop Rapid NGS for Public Health Microbiology March 4 th 8 th, 2013; Univ. Münster Sponsors In co-operation with MMGC 2013 1

Objectives and Organization Objectives It is our great pleasure to welcome you to the Workshop Rapid Next-Generation-Sequencing for Public Health Microbiology in Münster. Next-Generation-Sequencing (NGS) technology has developed rapidly during the last years. Innovative benchtop NGS platforms broaden application opportunities, especially for diagnostic use and surveillance. The program highlights opportunities and challenges posed by this technology. The workshop has been designed to boost practical knowledge exchange as well as ample discussions with your colleagues from all over Europe. Participation fees No participation fees will be charged for members of SeqNet.org, EU PathoNGenTrace & Antigone, BMBF German National Research Platform for Zoonoses, or DGHM Division of Population Genetics & Infectious Disease Epidemiology. Organization Dr. Dag Harmsen, Dr. Alexander Mellmann, Dr. Ulrich Dobrindt (Medical Faculty, University Münster) & Dr. Alexander Goesmann (CeBiTec, Bielefeld University). Registration and information Workshop places are limited. Therefore, please register before 21 st of January 2013. To do so, fill in the registration form (at the end of this document), sign it, and fax it to the following number: +49-(0)251-83 47134. Venue Department Periodontology, University Münster Waldeyerstr. 30, 48149 Münster Germany NOTE Save the dates! March 2014 we will organize in Münster, Germany again a larger Rapid NGS for Clinical & Public Health Microbiology conference (MMGC 2014). For 2013 we refer to the Wellcome trust Applied Bioinformatics and Public Health Microbiology conference (May 15 th - 17 th, 2013; Cambridge, UK). 2

Monday - Wednesday, 4 th 6 th March 2013 (max. 32 participants) 08.00-17.00 Ion Torrent PGM & Illumina MiSeq Microbial Whole Genome Shotgun (WGS) Sequencing Wet Laboratory Workshop including presentations & demonstrations of PGM and MiSeq laboratory workflow (e.g., amplicon & mate pair sequencing) and automatisation. Wednesday, 6 th March 2013 19.00-23.00 Evening Dinner in a typical Westphalian Cottage (max. 60 participants) Mühlenhof-Freilichtmuseum Münster (no fees). Thursday, 7 th March 2013 09.00-17.00 Rapid NGS for Public Health Microbiology Conference (max. 40 additional participants) See following page for speaker and topics. Friday, 8 th March 2013 09.00-17.00 Computer Class for NGS WGS Data Analysis CLCbio Genomics Workbench, BIGSdb, Ridom SeqSphere +, CLoVR, and MIRA(?) NGS WGS data analysis.

Speakers & Topics, Thursday, 7 th March 2013 (preliminary) Rapid NGS for Public Health Microbiology Jonathan Green, Health Protection Agency, Colindale, United Kingdom De-novo Assembly Strategies & MIRA (to be confirmed) Bastien Chevreux, Basel, Switzerland Benchtop NGS Platform & de-novo Assembler Comparison Sebastian Jünemann, CeBiTec Bielefeld Univ. & Univ. Münster, Germany WGS NGS Campylobacter spp. & BIGSdb Keith Jolley, Univ. Oxford, United Kingdom Manual & Automated Microbial Genome Annotation Alexander Goesmann, CeBiTec Bielefeld Univ., Germany NGS Cloud Computing & CLoVR W. Florian Fricke, Univ. Maryland, USA Reconstructing Disease Outbreaks from Pathogen Genome Sequences: a Bayesian Approach Thibaut Jombart, Imperial College London, United Kingdom WGS NGS Analysis of Neisseria and Pseudomonas spp. Ulrich Vogel, Univ. Würzburg, Germany WGS NGS Analysis of Staphylococcus aureus & Clostridium difficile Ulrich Nübel, Robert Koch Institute Wernigerode, Germany WGS NGS Analysis of Mycobacterium tuberculosis complex Stefan Niemann, Research Centre Borstel, Germany 4

Münster Transportation and Accommodation General Information Münster is a convention city in the German federal state of North Rhine-Westphalia with European tradition - envoys from Austria, France, Sweden, the Netherlands and Germany gathered there as early as 1648. In Münster City Hall they officially sealed the end of the Thirty Years War with the Treaty of Westphalia. Historical gabled buildings, baroque treasures, lots of green spaces and delightful surroundings all conspire to make visitors to Münster loath to say farewell. - 200 years of history, bikes, and a pulsating student community lend one of the largest German University Cities charm and character. How to get to Münster 1.) By plane Nearest airports: Münster/Osnabrück (IATA code: FMO) Airport is approx. 35 min by bus (line 51) Düsseldorf (IATA code: DUS) Airport is approx. 1.5 h by train (very convenient!) Hannover (IATA code: HAJ) Airport is approx. 2 h by train 2.) By train Münster is easy to reach by train via high-speed Intercity Express trains. Choose Münster Central Station ( Hauptbahnhof ) as your destination (http://www.bahn.de/i/view/usa/en/index.shtml). 3.) By car Münster is situated in the North-West of Germany. It is located directly at the A1 and A43 Autobahn. Accommodation Factory Hotel An der Germania Brauerei 5 48159 Münster, Germany WWW: http://www.factoryhotel-muenster.de/en/ From Münster Central Station via bus lines 6 or 15 (both from track B2 and direction: Kinderhaus Brüningheide) until Germania-Campus stop (approx. 15 min; 2.5 ) or via taxi (approx. 10 min; 10 ). We made a hotel room contingent reservation at the Factory Hotel that is valid until January 21 st 2013. If you register via phone (+49 [0]251 4188-0), fax (+49 [0]251 4188-999), or Email (mail@factoryhotel.de) by naming the code Münster Rapid NGS a room will be available for 89 per night (no price difference for 1 or 2 person occupation; exclusive breakfast). If you arrived in Münster, how do you find the Department of Periodontology? By bus/taxi The Department of Periodontology (named Zahnklinik on the following map) is located in the Waldeyer Street 30, 48149 Münster. From the Central Station: Busses leave every 10 min at the Central Station, i.e., line 11 (track C1), line 12 or 14 (both track B1). Suitable bus stops are Hüfferstiftung, Jungeblodtplatz, and Domagkstraße (takes about 12 min drive). A travel with a taxi from the Central Station takes about 10 min and costs about 10. From the Factory Hotel: The bus line 3 leaves at the Kanonierplatz (5 min walk from the hotel; direction Uni- Klinikum ) in about 10 min to the stop Uni-Klinikum. By taxi the drive takes about 5 min (about 7.5 ). 5

Münster Transportation and Accommodation 6

Please register before 21 st of January 2013. To do so, please fill in the following form completely, sign it, and fax it back to (we will confirm your participation within 3 working days; book only your accommodation after confirmation). Fax-number +49(0)251-83 47134 Ms. Mr. Registration Rapid NGS for Public Health Microbiology Title Last name Surname Institute / Clinic / Organisation Street ZIP City Country Phone Fax Email Workshop Rapid NGS for Public Health (max. 32 participants; March 4 th 8 th 2013) ONLY Conference Rapid NGS for Public Health (max. 40 additional participants; Thursday, March 7 th 2013) Social Event Mühlenhof (max. 60 participants; Wednesday evening, March 6 th 2013) If Workshop Rapid NGS for Public Health already occupied, then make at least a reservation for the Conference Rapid NGS for Public Health (March 7 th 2013) City Date Signature Contact Heike Frieling-Braithwaite Department Periodontology University Münster, Germany Phone: +49(0)251-83 47059 Fax: +49(0)251-83 47134 Email: Heike.Frieling-Braithwaite@ukmuenster.de