Advanced Instrumentation Research Facility

Advanced Instrumentation Research Facility USER CHARGES (All s to be prepared by users at their end unless specified) Please consult concerned AIRF staff member(s) before bringing your s. w.e.f 01.01.2015 Equipment/ Transmission Electron (TEM) Scanning Electron (SEM) (a) Biological Sample Preparation (preparation of blocks, sectioning and staining): Rs. 800/- per (b) Sample Preparation for Non- Biological Samples or Negative Staining: Rs. 500/- per (c) TEM Viewing: Rs. 500/- per (d) TEM Viewing & EDX: Rs. 700/- per (e) TEM Viewing & EDX (Mapping): Rs.1000/- per Coating: Rs. 200/- per Biological Sample Preparation (Fixation, dehydration, critical point drying and coating): Rs. 400/- per a) High Vacuum Mode: SEM Viewing: Rs.200/- per SEM Viewing & EDX: Rs. 500/- per Multipoint (>5) ; Rs. 800/- SEM Viewing & EDX Mapping: Rs. 800/- per b) Low Vacuum Mode: Viewing: Rs. 500/- per Institutions/IITs/IISERs/ Universities (a) Biological Sample Preparation (preparation of blocks, sectioning and staining): Rs. 2000/- per (b) Sample Preparation for Non- Biological Samples or Negative Staining: Rs. 800/- per (c) TEM Viewing: Rs.1500/- per (d) TEM Viewing & EDX: Rs. 2000/- per (e) TEM Viewing & EDX (Mapping): Rs.3000/- per Coating: Rs. 400/- per Biological Sample Preparation (Fixation, dehydration, critical point drying and coating): Rs. 1000/- per a) High Vacuum Mode: SEM Viewing: Rs. 600/- per SEM Viewing & EDX: Rs. 1500/- per Multipoint (>5) ; Rs. 2000/- SEM Viewing & EDX Mapping: Rs. 2000/- per b) Low Vacuum Mode: Viewing: Rs. 1000/- per Profit Laboratories (a) Biological Sample Preparation (preparationof blocks, sectioning and staining): Rs. 5000/- per (b) Sample Preparation for Non- Biological Samples or Negative Staining: Rs. 1500/- per (c) TEM Viewing: Rs.3000/- per (d) TEM Viewing & EDX: Rs. 5000/- per (e) TEM Viewing & EDX (Mapping): Rs. 7,000/- per Coating: Rs. 600/- per Biological Sample Preparation (Fixation, dehydration, critical point drying and coating): Rs. 2500/- per a) High Vacuum Mode: SEM Viewing: Rs.2000/- per SEM Viewing & EDX: Rs. 3600/- per Multipoint (>5) ; Rs. 4000/- SEM Viewing & EDX Mapping: Rs. 4000/- per b) Low Vacuum Mode: Viewing: Rs. 3000/- per Ten images shall be provided per. Hereafter, Rs. 100/- would be charged extra per image. Biological s will be accepted only after primary fixation with suitable fixative. For TEM, s should be trimmed into 1.0-1.5 mm thick pieces (4-5 pieces for each ). Fixation may be done in 2.5 % Glutaraldehyde and 2 % paraformaldehyde made in 0.1 M sodium phosphate buffer (ph7.2). The fixed should be brought at AIRF, in fixative or in phosphate buffer at 4 C preferably between 10 am to 1 pm (Working days). Only 10 s per requisition form will be accepted. Ten images per would be provided. Hereafter, Rs. 75/- would be charged extra per image. Biological s will be accepted only after primary fixation with suitable fixative. The thickness of s may be upto 1 cm 3. Fixation may be done in 2.5 % Glutaraldehyde and 2% paraformaldehyde made in 0.1 M sodium phosphate buffer (ph 7.2). The fixed should be brought at AIRF, in fixative or in phosphate buffer at 4 C preferably between 10 am to 1 pm (Working days). Only 10 s per requisition form will be accepted.

Confocal Inverted Research Fluorescence Rs. 3 0 0/- per hour for all s including FRET, FRAP and FCS _ Academic & Research Institutions/IITs/IISER s/ Universities Rs. 1500/- per hour for all s including FRET, FRAP and FCS _ Rs. 5000/- per hour for all s including FRET, FRAP and FCS _ submitting s. This has a unique two- tiered, V shape optical design to accommodate a wide range of advanced research techniques giving bright images with minimal reflections or frame modifications. The IX71's modular frame and optical design provides nine access ports for multiple input or output devices. Up to four ports can have simultaneous access to a primary image. All the four ports are IR compatible. The camera system on this IX71 platform is DP71. It has special features: 1. 1.45 megapixel high resolution (1360x1024 pixels) images displayed live at 15fps. 2. DP71's dedicated 2/3 inch CCD is cooled by a Peltier element to 10 0 C below ambient, ensuring high sensitivity, low noise image capture. 3. You can choose and apply settings easily according to the specimen and observation method and then capture images quickly. Time Resolved Fluorescence Spectrometer (TRFS) Rs. 150/- per hour or 1000 per day Rs. 250/- per hour or 2500 per day Rs. 1000/- per hour or per day Those users who have booked the instrument for whole day, but could not use it for more than 2-3 hours on the booking day, will be charged for complete day and Outside users who will use the instrument after a full day booking it will be charged as hourly basis thereafter ( 8 hrs.) Contact AIRF: i) Before preparing s. ii)for available excitation wavelengths. LIquid Nitrogen (LN2) Rs. 20/- per litre Rs. 50/- per litre Rs. 100/- per litre Contact AIRF.

FTIR-Raman FTIR: Rs. 200/- per RAMAN: Rs. 250/- per Institutions/IITs/IISERs/ Universities FTIR: Rs. 500/- per RAMAN: Rs. 600/- per FTIR: Rs. 1000/- per RAMAN: Rs. 1500/- per submitting s. IR-: Rs. 300/- per IR-: Rs. 1000/- per IR-: Rs. 2000/- per EDXRF i)pellet Form Rs. 400 /- per Rs. 100 /- per element ii) Loose Form Rs. 400 /- per Rs. 150 /- per element B) Aerosol Filters i) Pellet Form Rs. 1000 /- per Rs. 200 /- per element ii) Loose Form Rs. 1000 /- per Rs. 250 /- per element B) Aerosol Filters i)pellet Form Rs. 2000 /- per Rs. 500 /- per element ii) Loose Form Rs. 2000 /- per Rs. 600 /- per element B) Aerosol Filters preparing / submitting s Only solid s in powder form with particle size<75µm can be analyzed with better accuracy Minimum quantity of 1gm in case of plants and 2-3 gm in case of soil Samples should be properly dried Users should specify the elements to be identified For very accurate and precise quantitative measurements, the user should provide the respective standards Rs. 400/- per Calibration per element Rs. 100 /- per element Rs. 1000/- per Calibration per element Rs. 300 /- per element Rs. 1500/- per Calibration per element Rs. 600 /- per element For liquid s, 10ml liquid is required for C)Liquid Samples Rs. 600 /- per C)Liquid Samples Rs. 1500 /- per C)Liquid Samples Rs. 2000 /- per Rs. 150 /- per element Rs. 300 /- per element Rs. 1000 /- per element WDXRF i)pellet Form Rs. 400 /- per Rs. 100 /- per element i) Pellet Form Rs. 1000 /- per Rs. 300 /- per element i)pellet Form Rs. 2000 /- per Rs. 600 /- per element preparing / submitting s Only solid s in powder form with particle size<75µm can be analyzed with better accuracy Minimum quantity of 1gm in case of plants and 2-3 gm in case of soil Samples should be properly dried Users should specify the elements to be identified For very accurate and precise quantitative measurements, the user should provide the

X-Ray Diffraction (XRD) System for Powder Thin Films with low temperature attachment XRD at variable temperature Rs. 200/-per Institutions/IITs/IISERs/ Universities Rs. 800/-per Rs. 500/-per and per scan at Rs. 1000/-per and per scan different temperatures Rs. 200/- at different temperatures Rs. 300/- Rs. 1000/-per Rs. 2000/-per and per scan at different temperatures Rs. 500/- Sample should be crushed and brought in powder form Only 6 s per requisition form will be accepted Maximum of one requisition form per user will be accepted in one single day. Subsequent requisition forms of same user will be accepted for booking and only after all other users booked for the day, have finished their The maximum time for of one is one hour. After every one hour, the same would be treated as new and charged accordingly. Protein Crystallization for XRD Rs. 300/- per Consumables: Rs. 200/- Plates: Rs. 200/- per plate Rs. 400/- per Consumables: Rs. 200/- Plates: Rs. 300/- /- per plate Rs. 1000/- per Consumables: Rs. 1000/- Plates: Rs. 500/- per plate submitting s. X-ray Diffractometer (XRD) - for Macromolecules & Proteins Capillary Electrophoresis Rs. 500/- per Rs. 1500/- per Rs. 3000/- per Patterns and convolutions would be given. Rs. 200/- per three s Rs. 400/- per three s Rs. 1000/- per three s submitting Contact AIRF s. before Gas Chromatograph GC: Rs. 150/- per MS: Rs. 300/- per Mass Spectrometer GC-MS: Rs. 450/- per Note:- For any additional (GCMS) fragmentation spectra extra Multivapour charges of Rs. 30 per peak Rs. 50/- per GC: Rs. 400/- per MS: Rs. 800/- per GC-MS: Rs. 1000/- per Note:- For any additional fragmentation spectra extra charges of Rs. 30 per peak Rs. 100/- per GC: Rs. 1000/- per MS: Rs. 1500/- per GC-MS: Rs. 2500/- per Note:- For any additional fragmentation spectra extra charges of Rs. 30 per peak Rs. 150/- per Contact submitting AIRF s. before Submitting s. Output to user for GC = Peak Separation profile + RT + Area. For GC-MS = peak separation profile + RT + Area + Base peak fragmentation profile. For any additional fragmentation spectra extra charges of Rs. 30/- per peak. Conditions:- (a) User will provide detailed method protocol. (b) User will supply standards as per their requirement. (c) Interpretation of fragment spectra of MS is User s responsibility.

500 MHz Nuclear Magnetic Resonance (NMR) Spectrometer with solid state attachment (CPMAS) a) 1H NMR: Rs. 150/- per b) 13C and other Nuclei: Rs. 200/- per c) Experiments requiring hourly blocks (1 block for 3 hours): 1) Block: Rs. 300/- 2) Block: RS. 400/- 3) Block: RS. 500/- 4) Block: RS. 600/- d) 2D experiments: Rs. 400/- e) Solid-state experiments: i) 1H NMR: Rs. 500/- per ii) 13C NMR and other nuclei: Rs. 600/- per f) Variable Temperature:* i) High Temp.: Rs. 100/- ii) Low Temp. Rs. 200/- * charges to be added with experiments a/b/c/d/e g) Additional Deuterated Solvent Charges: ** D2O/CDCl3, DMSO-d6 :Rs. 100/- per * * charges to be added with experiments a/b/c/d/e/f Institutions/IITs/IISERs/ Universities a) 1H NMR: Rs. 350/- per b) 13C and other Nuclei: Rs. 500/- per c) Hourly blocks are not available to external users d) 2D experiments: Rs. 1000/- e) Solid-state experiments: i) 1H NMR: Rs. 2000/- per ii) 13C NMR and other nuclei: Rs. 3500/- per f) Variable Temperature:* i) High Temp.: Rs. 300/- ii) Low Temp.: not available to external users * charges to be added with experiments a/b/d/e g) Additional Deuterated Solvent Charges: ** D2O/CDCl3: Rs. 120/- per ; DMSO-d6 Rs. 150/- per * * charges to be added with experiments a/b/d/e/f a) 1H NMR: Rs. 700/- per b) 13C and other Nuclei: Rs. 1200/- per c) Hourly blocks are not available to external users d) 2D experiments: Rs. 2500/- e) Solid-state experiments: i) 1H NMR: Rs. 7500/- per ii) 13C NMR and other nuclei: Rs. 10,500/- per f) Variable Temperature:* i) High Temp.: Rs. 500/- ii) Low Temp.: not available to external users * charges to be added with experiments a/b/d/e g) Additional Deuterated Solvent Charges: ** D2O/CDCl3: Rs. 250/- per ; DMSO-d6 Rs. 450/- per * * charges to be added with experiments a/b/d/e/f submitting s. Important Note for Solidstate s: (a) As a matter of AIRF policy, it has been decided that solid-state NMR experiments would be done three times a year. (b) Requests for solid-state experiments should be directly made to Dr. Pritam Mukhopadhyay, NMR faculty in-charge (pritam.jnu@gmail.com) or Dr. Ajai Kumar, operator in-charge (ajkumar33@gmail.com). (c) Due to shortage of trained personnel and complexity involved in solid-state NMR experiments, no special requests for emergency recording of solid-state s would be entertained. (d) Samples for solid-state NMR should be sent to AIRF, only after taking permission from the NMR faculty in-charge or operator incharge. (e) Nuclei to be recorded have to be clearly mentioned. (f) Samples should be finely powdered and a minimum amount of 125 mg should be sent. (g) Please check the revised rates and send the exact amount. Surface Plasmon Resonance (SPR) Spectrometer Rs. 500/- per day SPR bare gold discs: Rs. 2500/- Chemicals and other consumables: Rs. 250/- per experiment Vacuum filter assembly: Rs. 2000/- Rs. 1000/- per day SPR bare gold discs: Rs. 2500/- Chemicals and other consumables: Rs. 500/- per experiment Vacuum filter assembly: Rs. 2000/- Rs. 3000/- per day SPR bare gold discs: Rs. 2500/- Chemicals and other consumables: Rs. 1000/- per experiment Vacuum filter assembly: Rs. 2000/- preparing s. Galvanostat- Potentiostat for electrochemical measurements Rs. 200/- for each / Consumables: Rs. 250/- Rs. 200/- for each / Consumables: Rs. 500/- Rs. 1000/- for each / Consumables: Rs. 1000/- preparing s.

Mass Spectrometry MALDI based Institutions/IITs/IISER s/ Universities MALDI based MALDI based 1-Intact Mass/MW Determination :Rs-200/- 1-Intact Mass/MW Determination :Rs-400/- 1-Intact Mass/MW Determination :Rs-1200/- 2-Peptide Mass Fingerprinting(PMF) :Rs-300/- 2-Peptide Mass Fingerprinting(PMF) 2-Peptide Mass Fingerprinting(PMF) :Rs-800/- :Rs-2000/- 3-MS/MS Protein Id: Rs-500/- 3-MS/MS Protein Id: Rs-1200/- 3-MS/MS Protein Id: Rs-2500/- 4-LC-Maldi (Protein Id/PTMs): Rs- 1000/- 4-LC-Maldi (Protein Id/PTMs):RS- 3000 4-LC-Maldi (Protein Id/PTMs):Rs- 5000/- 5-iTRAQ :Rs-10,000/- 6-MS/MS (per Precursor ion) :Rs- 200/- LC-ESI-MS/MS based 1-Inact Mass/MW determination :Rs-300/- 2-UPLC-MS /MS (Qualitative):Rs-500/- 3-UPLC-MS/MS (Quantitative):Rs-2000/- 4-MS/MS (per Precursor ion):rs-300/- 5-RP/RP 2D-nano LC-MS/MS :Rs- 1500/- 5-iTRAQ :Rs-50,000/- 6-MS/MS (per Precursor ion) :Rs-400/- LC-ESI-MS/MS based 1-Inact Mass/MW determination :Rs-600/- 2-UPLC-MS /MS (Qualitative):Rs-2000/- 3-UPLC-MS/MS (Quantitative):Rs-5000/- 4-MS/MS (per Precursor ion):rs-600/- 5-RP/RP 2D-nano LC-MS/MS :Rs- 4000/- 5-iTRAQ :Rs-2.5 Lac/- 6-MS/MS (per Precursor ion) :Rs-1200/- LC-ESI-MS/MS based 1-Inact Mass/MW determination :Rs-1500/- 2-UPLC-MS /MS (Qualitative):Rs-4000/- 3-UPLC-MS/MS (Quantitative):Rs-10000/- 4-MS/MS (per Precursor ion):rs-1500/- 5-RP/RP 2D-nano LC-MS/MS :Rs- 10000/- OffGel Fractionator Rs. 1000/- per OffGel Fractionator Rs. 2000/- per OffGel Fractionator Rs. 3000/- per Bio Analyzer Rs. 1500/- per chip ( 12 ) Bio Analyzer Rs. 3000/- per chip ( 12 ) Bio Analyzer Rs. 4500/- per chip ( 12 )

Femtosecond Laser Facility (Femtosecon d Fluorescence Upconversion) Rs. 1000/- per day Or Rs. 200/- per hour Institutions/IITs/IISER s/ Universities Rs. 1500/- per day Or Rs. 300/- per hour Rs. 3000/- per day Please contact Dr. Sobhan Sen (faculty in-charge, Femtosecond facility) at sobhan.sen@gmail.com for details of preparation and other details. Instructions for the users: 1. The user has to run their s first in the picosecond TRFS machine. And only if it is absolutely necessary to obtain the fast life-times of molecules which are not resolved in picosecond TRFS machine, only then the users will be allowed to run their s in the femtosecond facility. 2. The users should have sufficient knowledge of picosecond and femtosecond spectroscopy. 3. Available excitation wavelengths are: 375 nm to 470 nm (for second harmonic). 280 nm to 320 nm (for third harmonic). 4. Lifetime measurement window: 50 fs to 1.5 ns (after deconvolution). Circular Dichroism (CD) Spectrometer Rs. 150/- per hour Rs. 500/- per hour Rs. 2500/- per hour submitting s. Stop Flow Rs. 100/- per Rs. 500/- per Rs. 1000/- per submitting s. Cell Sorter and Flow Cytometer Quartz Crystal Microbalance Rs. 400/- per hour for Flow cytometry (including the sheath fluid charges) Rs. 400/- per hour for Sorting Rs. 100/- for each / Quartz Crystal Ti/Au: Rs. 4200/- Consumables: Rs. 250/- per day Note: Rates of crystal may vary depending upon the specifications and Company price. Rs. 800/- per hour for Flow cytometry (including the sheath fluid charges) Rs. 1200/- per hour for Sorting Rs. 200/- for each / Quartz Crystal Ti/Au: Rs. 4200/- Consumables: Rs. 500/- per day Note: Rates of crystal may vary depending upon the specifications and Company price. Rs. 1500/- per hour for Flow cytometry (including the sheath fluid charges) Rs. 3000/- per hour for Sorting Rs. 1000/- for each / Quartz Crystal Ti/Au: Rs. 4200/- Consumables: Rs.1000/- per day Note: Rates of crystal may vary depending upon the specifications and Company price. Users will be provided the sheath fluid and tubes for their. submitting s. Physical property Measurement System (PPMS) Rs. 1000/- per day Or Rs. 100/- per hour Rs. 2000/- per day Or Rs. 250/- per hour Rs. 5000/- per day Submitting s.

Institutions/IITs/IISER s/ Universities Private Industries/ For- Live cell Confocal Rs. 600/- per hour TIRF Rs. 300/- per hour Rs. 2500/- per hour TIRF Rs. 1500/- per hour Rs. 4000/- per hour TIRF Rs. 3000/- per hour Contact AIRFbefore preparing s and for available facilities pertaining to live imaging. Instrument facilities available in this laboratory: Bio Station Rs. 300/- per hour Bio Station Rs. 1500/- per hour Bio Station Rs. 3000/- per hour 1. Real TimeLaser Scanning Confocal Model A1R with motorized inverted microscope having Live Cell and Spectral Imaging (Nikon- Model Ti-E) 2. Fully Motorized Laser Total Internal Reflection Fluorescence (TIRF) (Nikon- Model Ti- E) 3. Integrated Live Cell Imaging Set- up (Model Biostation IM- Q) 4. Trinocular Research (anti-fungus type) for bright field, phasecontrast, DIC, polarizing and digital camera attachment (Nikon- Model 80i) 5. Trinocular (anti fungus type) with digital camera system o All results would be given on a compact disc (CD) provided by AIRF. # Analysis other than routine shall be charged extra. ** AIRF will not supply any software copies for offline. *** The above rates are subject to time to time revision. Note: The facility timings are from 9.00 am to 5.30 pm. The Facility is closed on Saturday, Sunday and Government Holidays. The Requisition forms for the users can be downloaded from the AIRF site or can be collected from AIRF Office. For the users within, the duly filled forms must be signed by the Supervisor and submitted at the AIRF office for the booking of the user slot for instrument. The requisition forms from users outside must be signed by concerned officials and submitted in AIRF office or sent before by mail/ post/ courier along with the Demand Draft drawn in favour of The Finance Officer - payable at New Delhi for the amount calculated as per user charges given above for all s/. Users have to pay service tax with user charges according to service Tax act as 12.36 %. Users coming from outside and other stations may reconfirm the availability of time slots in advance just before coming. The Director s decision shall be final in case of any dispute. The users shall be allotted the time slots as per the availability. All users are required to acknowledge the use of AIRF equipment/ AIRF facilities and the person(s) providing the technical help in all their research publications/ articles resulting from the use of AIRF. A copy of such publication must be submitted to AIRF for reference and record.