FlexCycler 2. Thermal Cycler. Operating manual

FlexCycler 2 Thermal Cycler Operating manual

Manufacturer: Biometra GmbH Rudolf-Wissel-Str. 30 37079 Göttingen Germany Phone: + 49 (0) 551 / 50 68 6-0 Fax: + 49 (0) 551 / 50 68 6-66 Email: info@biometra.com Service: Analytik Jena AG Life Science Konrad-Zuse-Str. 1 07745 Jena Germany Phone: + 49 (0) 3641 / 77-94 11 Fax: + 49 (0) 3641 / 77-76 77 76 Email: k.fritzsche@analytik-jena.de General information about Analytik Jena AG on the internet: http://www.analytik-jena.de Documentation number: 34-3700-000-23 Edition November 2012 Implementation of the Technical Documentation: Analytik Jena AG This publication describes the state of this product at the time of publishing. It need not necessarily agree with future versions of the product. Modifications reserved! Copyright 2012 Analytik Jena AG

Contents Contents 1 Introduction... 5 1.1 Intended use... 5 1.2 Special features of the FlexCycler 2... 5 1.2.1 Block exchange system... 5 1.2.2 Temperature uniformity... 5 1.2.3 Heated lid... 5 1.2.4 Operation... 6 1.2.5 Software options... 6 1.2.6 User management... 6 1.2.7 Housing... 6 1.2.8 USB functions... 7 1.3 Technical specifications... 7 1.4 Legal information... 8 1.4.1 PCR license information... 8 1.4.2 Liability... 8 2 Safety instructions... 10 2.1 Meaning of the symbols used in the operating manual... 10 2.2 Safety symbols on the FlexCycler 2... 10 2.3 General safety instructions... 11 3 Installation... 13 3.1 Scope of delivery... 13 3.2 Unpacking and inspection... 13 3.3 Setting the operating voltage... 13 3.4 Commissioning... 14 3.5 Automatic power-on self test... 14 4 First steps... 15 4.1 Front view... 15 4.2 Rear view... 15 4.3 Exchanging the block module... 16 4.4 Control unit... 17 4.5 The High Performance Smart Lid (HPSL)... 17 Closing the lid... 17 Opening the lid... 18 5 Operation... 19 5.1 User interface... 19 5.1.1 Menu buttons... 19 5.1.2 Softkeys... 20 FlexCycler 2 Edition 11/2012 1

Contents 5.1.3 LOG IN menu... 20 5.1.4 BLOCK menu/quick-start function... 21 5.1.5 SYSTEM menu... 22 5.1.6 PROGRAM menu... 22 5.2 Software... 23 5.2.1 Log in... 23 5.2.2 Logging in a user... 23 5.2.3 Creating a new user directory... 25 5.2.4 Logging in as a supervisor... 25 5.2.5 Deleting a user directory... 26 5.3 Creating a new program... 27 5.3.1 Creating a program... 27 5.3.2 Selecting the block type... 27 5.3.3 Heated lid settings... 28 5.3.4 Setting the lid temperature... 29 5.3.5 Entering the temperature and hold time for a step... 29 5.3.6 Defining a loop... 30 5.3.7 Cooling the samples after the run... 31 5.3.8 Programming a pause... 31 5.3.9 Saving the program... 32 5.3.10 Programming the temperature gradient... 33 5.3.11 Programming a linear gradient... 34 5.4 Setting program options... 35 5.4.1 Setting the heating and cooling rate... 36 5.4.2 Changing the hold time in cycles Δt(s)... 36 5.4.3 Changing the temperature in cycles ΔT( C)... 37 5.5 Editing programs... 37 5.5.1 Inserting a step... 38 5.5.2 Deleting a step... 39 5.5.3 Copying the program... 40 5.5.4 Deleting a program... 41 5.5.5 Editing programs in the graphical mode... 42 5.6 Starting a program... 43 5.6.1 Selecting a program for the start... 43 5.6.2 User-specific quick-start of programs... 44 5.6.3 Program preview... 45 5.6.4 Display during a run... 46 5.6.5 Pausing the program... 48 5.6.6 Continuing the program... 49 2 Edition 11/2012 FlexCycler 2

Contents 5.6.7 Skipping a program step... 49 5.7 Stop the program... 50 5.7.1 Stopping a program in pause... 51 5.8 USB functions... 51 5.8.1 Connecting the USB stick with the thermal cycler... 51 5.8.2 Opening the USB folder... 52 5.8.3 Deleting a program in the USB folder... 52 5.8.4 Copying the program from the USB folder... 53 5.8.5 Copying a program to the USB folder... 54 5.8.6 Maximum number of programs in the USB folder... 55 6 System settings... 56 6.1 User configuration... 56 6.1.1 Setting the language... 56 6.1.2 Changing the PIN code... 57 6.1.3 Deleting a user directory... 57 6.2 System configuration... 58 6.2.1 Setting the acoustic signal... 58 6.2.2 Setting the contrast... 59 6.2.3 Setting the date and time... 59 6.2.4 Defragmenting the memory... 59 6.2.5 Setting the screen saver... 60 6.3 System information... 60 6.3.1 System information... 61 6.3.2 Viewing/saving the log files from the last nine runs... 61 6.4 SERVICE menu... 62 6.4.1 Displaying log files of the "Power-on" self test... 62 6.4.2 Performing an extended self test... 63 6.4.3 Displaying log files of the extended self test... 64 6.4.4 Displaying the error history... 65 6.4.5 Creating an info file for Analytik Jena Service... 65 6.5 Contacting Analytik Jena... 66 6.6 Supervisor system settings... 66 6.6.1 Changing a user's PIN code... 66 6.6.2 Deleting a user directory (user account)... 67 6.6.3 Changing the supervisor PIN code... 67 6.6.4 Setting the boot language... 68 6.6.5 Deleting all user directories (user accounts)... 68 7 Quick reference guide... 69 Safety instructions... 69 FlexCycler 2 Edition 11/2012 3

Contents The FlexCycler 2 control panel... 69 Log in... 69 Creating, modifying and saving programs... 70 The FlexCycler 2 programming table... 70 Starting, pausing and stopping programs... 72 8 Troubleshooting... 74 8.1 Slow heating and cooling... 74 8.2 Auto restart... 74 8.3 Auto restart without recognizable cause... 74 8.4 Adjusting protocols of other thermal cyclers... 75 8.5 Loosening the lid wheel in case of blockage... 75 9 Maintenance and care... 76 9.1 Cleaning and decontamination... 76 9.2 Service and repair... 76 9.3 Firmware update... 76 9.4 Spare parts... 76 10 Order numbers for FlexCycler 2 and block modules... 77 11 Service... 78 11.1 Device return information... 78 11.2 Packaging the thermal cycler... 79 12 Disposal... 80 13 Declaration of conformity... 81 14 Warranty... 82 15 Index... 83 Figures Figure 1 Voltage selector switch on the bottom of the device... 13 Figure 2 Front view of the FlexCycler 2... 15 Figure 3 Connections and block release lever on the rear of the FlexCycler 2... 15 Figure 4 Block exchange... 16 Figure 5 Setup of the control unit... 17 Figure 6 Diagram for inserting empty tubes into different block systems... 18 4 Edition 11/2012 FlexCycler 2

Introduction 1 Introduction 1.1 Intended use The FlexCycler 2 is a thermal cycler designed for multiplying nucleic acids by means of PCR. The FlexCycler 2 is licensed for this type of application (research use only). It performs cyclically repeated temperature changes to create the adequate environmental conditions. With the help of enzymes for DNA amplification (polymerases) as well as additional required components in an aqueous solution, nucleic acid sequences can be copied in suitable reaction tubes. The device can further be used for other applications such as ligation, nucleic acid digestion by means of restriction enzymes, bisulfite conversion or protein crystallography, all of which require exact temperature control. 1.2 Special features of the FlexCycler 2 1.2.1 Block exchange system Thanks to the Quick-X-Change technology, the block modules in the FlexCycler 2 can be exchanged in just a few seconds. The built-in quick block exchange system works without using additional tools or screw connections. Just lift the block exchange lever, remove the block to be exchanged, insert the new block and lower the lever to establish the connection to the base unit. The device automatically detects and installs the newly inserted block. Thanks to the block exchange function of the FlexCycler 2 the device configuration can be changed within a few seconds, if necessary. But not only single block modules are available; there are also the twin block modules that feature two independently controllable blocks and heated lids. With the twin block modules you can run two different programs at the same time, allowing an even more flexible operation. Optionally, blocks with additionally featuring the gradient function are available. These can be used for the quick and comfortable optimization of new PCR assays. 1.2.2 Temperature uniformity All FlexCycler 2 blocks feature high-quality platinum sensors with a temperature accuracy of ± 0.1 C. 1.2.3 Heated lid The heated lid of the FlexCycler 2 features the High Performance Smart Lid (HPSL) technology, which guarantees the formation of a homogeneously tempered air cushion between the samples. The device therefore offers an excellent temperature uniformity over the whole block and reproducible PCR conditions regardless of the positioning of the samples. At the same time, an integrated friction clutch makes sure that the contact pressure always remains the same, even if the shape and height of the plastic ware change. The uniform distribution of the pressure on all tubes/wells ensures that the reaction tubes are securely closed during the PCR and that the temperature transition between block and reaction mix is ideal. In addition, evaporation and condensation effects are prevented. FlexCycler 2 Edition 11/2012 5

Introduction 1.2.4 Operation With the clearly structured FlexCycler 2 operating software, temperature programs can be programmed in a well-arranged table format (Easy Spreadsheet Programming (ESP)). All necessary parameters can be entered in an easy-to-navigate window. There is no need to call up subwindows to set variables for specific program functions or to toggle between several windows. Optionally, with the single touch of a button the temperature profile can be displayed graphically and parameters modified. The FlexCycler 2 has a memory capacity for more than 300 programs. For a clear overview, the FlexCycler 2 has a user-specific quick-start option that displays the five most recently used or edited programs. The user who is logged in to the system is presented a list from which the desired program can be started directly by pressing the start key on the front panel. The FlexCycler 2 also features a multiblock selection tool for starting and stopping programs. Therefore, programs can also be started and ended on several blocks at the same time. 1.2.5 Software options Aside from programming temperature protocols, the software features several other useful functions. It has an extended self test, is able to display run log files for GLP-complient documentation of PCR runs and can create service info files in service cases. When the extended self test is active, the FlexCycler 2 automatically tests itself and summarizes the results in a clearly structured report. If a section of the test has been failed, the user receives a corresponding message. 1.2.6 User management The FlexCycler 2 manages up to 30 different user directories that can optionally be protected by a PIN code. Users are not able to change temperature programs of other users in protected directories. In addition to normal user directories, the supervisor (administrator) there is a second user level with extended rights. The supervisor has his own menu for managing the system and is able to delete user directories. (This also includes protected directories.) The supervisor can also select the boot language of the system. 1.2.7 Housing The housing of the FlexCycler 2 impresses by its distinct unique design, clean lines, and its functionality. The high quality of the device also makes it suitable for routine continuous operation. One example of its advantages is the air flow within the device: It is designed to dissipate excessive heat as quickly as possible. This keeps energy consumption low and, at the same time, the temperature uniformity of the block remains at an optimal level. In addition, its compact design means that the FlexCycler 2 needs very little space in the laboratory. The display and keyboard are arranged in an ergonomically angle, allowing the comfortable operation of the instrument and also preventing unwanted light reflections in the display from the surroundings. 6 Edition 11/2012 FlexCycler 2

Introduction 1.2.8 USB functions The USB functions can be used to exchange temperature programs between different FlexCycler 2 devices, save run log files for documenting PCR runs and create service info files (SINF) in service cases. For this purpose, the device has a USB A port for connecting standard USB sticks. The USB B port at the rear of the FlexCycler 2 can be used to copy software updates from a connected computer easily and conveniently. It is not necessary to open the thermal cycler or remove parts to update the instrument. 1.3 Technical specifications Order number Capacity 844-00062-x 844-00063-x FlexCycler 2 twin 48 FlexCycler 2 twin 48G 2 x 48 x 0.2 ml tubes, 2 x 6 x strips of 8 0.2 ml or 2 x 48 well micro titer plates 844-00060-x 844-00061-x FlexCycler 2 96 FlexCycler 2 96G 96 x 0.2 ml tubes, 6 x strips of 8 0.2 ml or 96 well micro titer plates Block material Aluminum Aluminum Block coating Anodized silver Anodized silver Block exchange Quick-X-change Quick-X-change Block exchange time under 10 s under 10 s Maximum heating rate* 4.5 C/s 4.0 C/s Maximum cooling rate* 4.5 C/s 4.0 C/s Average heating rate* 3.5 C/s 3.0 C/s Average cooling rate* 3.5 C/s 3.0 C/s Mamium gradient span** 20 C 30 C Temperature uniformity Twin 48 95 C: ± 0.60 C within 15 s 70 C: ± 0.30 C within 15 s 55 C: ± 0.25 C within 15 s Twin 48G 95 C: ± 0.70 C within 15 s 70 C: ± 0.40 C within 15 s 55 C: ± 0.15 C within 15 s Temperature range 3 C 99 C Gradient temperature 20 C 99 C range Control accuracy +/- 0.1 C Software Programming modes Memory 95 C: ± 0.60 C within 15 s 70 C: ± 0.40 C within 15 s 55 C: ± 0.20 C within 15 s Quick-start of the last 5 programs, program preview, toggle between program table and graphical programming, graphical gradient input, variable heating and cooling rates, extended self test, service files for remote diagnosis, USB functions such as saving programs, run log files or SINF files. Easy spreadsheet or graphical mode Approx. 350 programs in 30 user directories with optional PIN code protection. FlexCycler 2 Edition 11/2012 7

Introduction Display Auto restart function Smart lid technology ¼ VGA screen, 320 x 240 pixels Yes Yes Lid temperature range 30 C 99 C Power consumption Operating voltage 600 watt 100, 115, 230 V, 50 60 Hz Weight 15.0 kg 15.3 kg Dimensions (width x height x depth) Noise emissions Interfaces Environmental conditions 26.4 cm x 28.9cm x 40.0 cm 26.4 cm x 47.9cm x 40.0 cm with open lid Very low USB A, USB B 15 C 35 C, 70 % air humidity, max 2000 m NN * measured in the block ** Only for gradient enabled block modules. For twin block modules the left block (block 1) is gradient enabled. 1.4 Legal information 1.4.1 PCR license information Purchase of the FlexCycler 2 conveys a limited non-transferable immunity from suit for the purchaser s own internal research and development and applied fields other than human in vitro diagnostics under one or more of US Patents Nos. 5,038,852, 5,656,493, 5,333,675, 5,475,610, and 6,703,236, or corresponding claims in their non-us counterparts, owned by Applera Corporation. No right is conveyed expressly, by implication or by estoppel under any patent claim, reagents, kits, or methods such as 5 nuclease methods, or under any other apparatus or system claim, including but not limited to US Patent No. 6,814,934 and its non-us counterparts, which describe and claim thermal cyclers capable of real-time detection. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA. Analytik Jena AG Konrad-Zuse-Strasse 1 07745 Jena Germany 1.4.2 Liability The warranty and liability period comply with the legal requirements and the provisions in the general terms and conditions of Analytik Jena AG. Deviations from the intended use described in this user manual result in limitations of warranty and liability in case of damage. Damage to wearing parts is not included in the warranty. 8 Edition 11/2012 FlexCycler 2

Introduction Warranty and liability claims are excluded for personal injury and property damage due to one or several of the following causes: use of the FlexCycler 2 other than intended improper commissioning, operation and servicing of the device modifications of the equipment without prior consultation with Analytik Jena AG unauthorized intervention in the equipment operation of a faulty device use of other than original spare parts, wearing parts or consumables improper repairs faults due to the non-observance of this user manual FlexCycler 2 Edition 11/2012 9

Safety instructions 2 Safety instructions 2.1 Meaning of the symbols used in the operating manual CAUTION! Hazard area! Indicates a potentially hazardous situation which, unless avoided, may result in light or minor injuries. CAUTION! Risk of electric shock! Do not touch! Indicates a hazardous situation which, unless avoided, may result in electric shock or damage to the device (short-circuit). ATTENTION Indicates a potentially hazardous situation which, unless avoided, may cause damage to the device or items in its vicinity. IMPORTANT Indicates application hints and other especially useful information without any resulting hazardous or damaging situations. 2.2 Safety symbols on the FlexCycler 2 Damaged or missing safety symbols can cause incorrect actions leading to personal injury or material damage. Do not remove safety symbols! Replace damaged safety symbols immediately! The following safety symbols are attached to the inside of the heated lid and the rear of the device: CAUTION! Hazard area! Proceed with care when handling samples and working with the FlexCycler 2. CAUTION! Hot surfaces! There is a risk of burning at the heated lid, the thermal block, the samples, and the rear of the device. The following signs are attached to the bottom of the FlexCycler 2 : ATTENTION! Keep ventilation slots clear! Keep the ventilation slots on the bottom and at the rear of the device clear. Make sure that there are no objects underneath the device (e.g. paper) that may be sucked against the ventilation slot thus interfering with ventilation. 10 Edition 11/2012 FlexCycler 2

Safety instructions 2.3 General safety instructions Carefully read the operating instructions before using the FlexCycler 2. Intended use The FlexCycler 2 is designed for incubating samples with alternating temperatures and is to be used for this purpose only. Local regulations Local safety regulations that apply to the use of this device (e.g., work protection regulations, accident prevention regulations, and accident avoidance regulations) must be observed. References to potential dangers in the handbook do not replace the work protection regulations which must be observed. Requirements for the operating personnel The FlexCycler 2 may only be operated by qualified personnel, who have received additional training for this type of work. The instruction also includes conveying the contents of this user manual. Any work on electrical equipment may only be carried out by qualified electricians. CAUTION! Risk of burns! The thermal block, the samples and the heated lid reach high temperatures. There is a risk of burns during contact. Do not touch the heated lid! The rapid heating of the thermal block can cause liquids to boil explosively. This is why you always need to wear goggles during operation! Before starting a program close the lid of the device. Ensure that the lid is securely closed before starting the program! The samples can reach high temperatures. Do not touch hot sample tubes or plates and do not open them or boiling liquid might splash out! Only use plates and tubes that are suitable for high temperatures (as of 100 C), fit well into the thermal block (no shaking) and whose lids seal tightly! CAUTION! Risk of electric shock! Do not touch! Do not use the FlexCycler 2 if there is any visible damage to the block, housing, cables or any other components. Before starting the device, make sure that the voltage selector on the bottom is set to the right mains voltage (see section "Setting the operating voltage" p.13). Always disconnect the mains plug before opening the housing. Connect the FlexCycler 2 to a grounded mains. Mains plug and mains switch must be easily accessible. FlexCycler 2 Edition 11/2012 11

Safety instructions CAUTION! Safety instructions during operation When handling pathogenic, radioactive or infectious samples, the relevant safety regulations must be observed. For details contact the safety officer responsible for your location. The FlexCycler 2 must not be operated with flammable, explosive or volatile substances. Do not place your fingers between the block and lid when opening or closing the lid. Risk of crushing! If only few samples are being incubated, 2 additional (empty) tubes of the same height must be placed at each corner of the block. If the number of tubes in the block is too low, there is a risk of the tubes being damaged. Before opening the lid, reduce the contact pressure (see section "The High Performance Smart Lid (HPSL)" p. 17). It is not necessary to use oil between the samples and the block to create a better heat exchange. If you do wish to use oil, never use silicone oil but mineral oil instead. Make sure that the ventilation slots at the bottom and rear of the device are unobstructed and not clogged with dust or other materials. Risk of overheating! If the FlexCycler 2 has been moved, allow the device to adjust to room temperature before switching it on for the first time (1 to 6 hours). Place the device at a minimum distance of 10 cm between the rear of the device and the wall or other objects. Risk of overheating! The device has been built and certified according to safety standard EN 61010-1. Do not modify the device in any way. Any modification of the device will lead to a loss of warranty and the EN 61010-1 certificate and poses a potential risk. Place the FlexCycler 2 on a solid, non-flammable surface in a secure, dry environment. For details please refer to the environmental conditions in the section "Technical specifications" p. 7. CAUTION! Safety instructions for maintenance and transport The FlexCycler 2 does not contain any parts that can be serviced or repaired by the operator. Do not open the housing of the thermal cycler! Service and repair work should be performed by Analytik Jena AG Service or by trained qualified personnel. Do not use alcohol (e.g. methanol or ethanol), organic solvents or abrasives to clean the device. Always transport the device in the original box. CAUTION! Behavior during an emergency If there is no immediate risk of injury, immediately switch the device switch of the FlexCycler 2 into the "0" position during dangerous situations or accidents if this is possible and/or disconnect the mains plugs from the mains! 12 Edition 11/2012 FlexCycler 2

Installation 3 Installation 3.1 Scope of delivery 1. Base unit with exchangeable block module 2. Mains cable 3. Operating instructions 3.2 Unpacking and inspection Unpack and carefully check the device. Immediately report any damage to Analytik Jena AG. Do not attempt to operate the device if there is any visible damage. Keep the original box for possible return. The FlexCycler 2 must always be transported in its original packaging. If the packaging is no longer available, please contact Analytik Jena AG. 3.3 Setting the operating voltage CAUTION! Risk of electric shock! Do not touch! Before commissioning the device, make sure that the voltage selector setting on the bottom of the device corresponds to the mains voltage in your laboratory. The FlexCycler 2 can be operated at 100, 115 or 230 volts. Set the operating voltage by turning the dial on the bottom of the FlexCycler 2 to the desired voltage. You can do so with a coin. Figure 1 Voltage selector switch on the bottom of the device FlexCycler 2 Edition 11/2012 13

Installation 3.4 Commissioning CAUTION! Danger of electric shock! Prior to commissioning, please make sure that the operating voltage set on the device is the same as the mains voltage (see section "Setting the operating voltage" p. 13). ATTENTION! Do not cover the air vents! Ensure that the air vents are clear. Insufficient ventilation may cause the device to overheat. Make sure that there are no objects underneath the device (e.g. paper) that may be sucked against the ventilation slot thus interfering with ventilation. Place the device at a minimum distance of 10 cm between the rear of the device and the wall or other objects. Place the FlexCycler 2 on a solid dry surface. If the FlexCycler 2 has been moved, allow the device to adjust to room temperature before switching it on for the first time (1 to 6 hours). Connect the FlexCycler 2 with a grounded mains outlet using the provided mains cable. Make sure that the mains switch and the mains cable are accessible. This is important if the device needs to be disconnected from the mains voltage. The display contrast can be adjusted according to the respective lighting conditions (see section "Setting the contrast" p. 59). 3.5 Automatic power-on self test When the device is switched on, it displays a serial number and software version and performs a self test. During this test, it checks whether all important functions are working properly. The results from the initial self test are saved on the thermal cycler and can be accessed again later (see section "Displaying log files of the "Power-on" self test" p. 62). 14 Edition 11/2012 FlexCycler 2

First steps 4 First steps 4.1 Front view Figure 2 Front view of the FlexCycler 2 4.2 Rear view Figure 3 Connections and block release lever on the rear of the FlexCycler 2 FlexCycler 2 Edition 11/2012 15

First steps 4.3 Exchanging the block module ATTENTION! Do not touch the contacts! Avoid touching the plug contacts on the block or the device. It is possible that the electronic system may be damaged due to electrostatic discharge. 1. Switch off the device when exchanging the block. 2. Flip up the lever on the rear of the device (1) and slowly pull the block module back (2). 3. As soon as the plug connection at the front is released, the module can be lifted up (or back) and out. For installing a block module, proceed in reverse order: 4. Carefully insert the module into the device from the rear / top and slowly push it forward, until the plug clicks into the contacts (2). 5. Carefully push the lever on the rear down (1) until the module is firmly connected to the device. 6. Switch on the FlexCycler 2. During start-up the module is automatically recognized and the block format and serial number are displayed. Figure 4 Block exchange 16 Edition 11/2012 FlexCycler 2

First steps 4.4 Control unit Figure 5 Setup of the control unit 4.5 The High Performance Smart Lid (HPSL) The FlexCycler 2 has a height-adjustable lid for optimal contact pressure on the samples. Closing the lid 1. Place the samples in the block, then close the lid until you feel the button in the front click into position. 2. Turn the lid wheel clockwise until the friction clutch activates (you will hear a clicking noise). ATTENTION! The strength of the lid contact pressure has been designed for a fully loaded block. If only a few samples are to be used in the block, please place an additional two (empty) tubes of the same height in the four corner positions of the block. Otherwise the sample t may be damaged from excess contact pressure (see also Figure 6 below). Attention! Never attempt to close the heated lid with force! This may damage the device. FlexCycler 2 Edition 11/2012 17

First steps Figure 6 Diagram for inserting empty tubes into different block systems Opening the lid ATTENTION! Never open the lid under pressure! Always follow the sequence below when opening the lid. If the lid is opened under pressure, the locking mechanism may be damaged. 1. Reduce the contact pressure of the lid completely by turning the lid wheel anticlockwise until there is no resistance. 2. Open the lid by pressing the button on the front of the lid. The lid opens and locks in a defined end position. 18 Edition 11/2012 FlexCycler 2

Operation 5 Operation 5.1 User interface The user interface of the FlexCycler 2 has four menu buttons above the screen and four softkeys below the screen. 5.1.1 Menu buttons The four menu buttons are for quick access to the four main menus of the FlexCycler 2. These are: the LOGIN/OUT menu the SYSTEM menu the BLOCK menu the PROGRAM menu The active menu is indicated by a graphical link to the corresponding menu button on the top edge of the screen. In this example the PROGRAM menu is active. The four menu buttons always have the same function. In contrast, the function of the softkeys changes depending on the software menu that is being accessed. The current function of the softkeys is always shown in the bottom row of the screen. FlexCycler 2 Edition 11/2012 19

Operation 5.1.2 Softkeys There are four softkeys below the screen. In contrast to the menu buttons the function of the softkeys changes depending on the program window (context-sensitive). The current function of the softkeys is always shown in the bottom row of the screen. 5.1.3 LOG IN menu After switching on (and booting) the FlexCycler 2, the LOG IN menu is displayed automatically. If the device is already switched on and is in another menu, press the [LOG IN/OUT] menu button above the screen to log into or out of the thermal cycler. To log in, select a user from the list or create a new user. The initials of the currently loggedin user will be shown in the status line at the top of the screen: 20 Edition 11/2012 FlexCycler 2

Operation 5.1.4 BLOCK menu/quick-start function The BLOCK menu displays data on the current status of the sample block. This section is particularly important during the run, because this is where the progress and remaining time are displayed. Press the [BLOCK] menu button above the screen access the block menu. The display differs depending on whether a block module with an individual block or a twin block module is installed: Monoblock Twinblock For twin block systems, the FlexCycler first displays an overview screen with the status of the two blocks. Each block can be active with a running program or free. Select block 1, block 2 or all blocks with the corresponding softkeys. The view changes to the overview of the last used programs as with the Monoblock. If the block is free (i.e. no program is running) up to five programs are listed that were last started on the block or edited by the user who is logged in to the system. The FlexCycler 2 here offers a comfortable quick-start function. Select the desired program from the list and press the [START] key on the control unit (see section "Control unit" p. 17). The corresponding program is started immediately. To display a program prior to starting, press the [VIEW PROGRAM] softkey. The programmed parameters and the calculated duration of the program are listed: Note: In PREVIEW mode it is not possible to edit programs. FlexCycler 2 Edition 11/2012 21

Operation 5.1.5 SYSTEM menu Press the [SYSTEM] menu button above the screen. The SYSTEM menu can be used to access the basic settings of the thermal cycler and to query protocol data and service files. The protocol files from the automatic start-up test can be called up here (see section "System settings" p.56). 5.1.6 PROGRAM menu Press the [PROGRAM] menu button above the screen. The PROGRAM menu is used to create and edit programs. There is an individual directory for saving programs for every user. The user directory is marked by initials. The programs from foreign, PIN code-protected user directories can be read, but they cannot be changed. If necessary, a program can be copied from another user directory to the current user directory and edited there. 22 Edition 11/2012 FlexCycler 2

Operation 5.2 Software The arrow keys can be used for navigation on all screens. The [LEFT] and [RIGHT] key have additional functions in some menus. These additional functions shall be pointed out inthis manual. The [RIGHT] arrow key moves the cursor into the next field. This key can also be used to confirm a data entry: During programming the entered value is accepted when the [RIGHT] key is pressed and the cursor jumps to the next field. In the directory tree, the [RIGHT] key moves the cursor into the next lower directory structure. The [LEFT] key moves the cursor to the previous field. On most screens the [LEFT] key is the same as the Back key. In the directory tree, the [LEFT] key moves the cursor into the previous, higher directory structure. 5.2.1 Log in The FlexCycler 2 manages up to 30 user directories. Every user has his or her own directory into which the programs are saved. Programs from foreign, PIN code-protected user directories can be read, but they cannot be changed. If a user wants to modify another user's program, he can copy it to his own directory and edit it there. Every user is identified by his name and his 2 or 3-digit initials. The initials are adopted to the system as an identification code for the individual user directory. Every user directory can be protected by a PIN code (1 7 digits). If you do not wish to have your directory PIN-code protected, leave the "ENTER PIN" field empty and continue with [EN- TER]. Note: Without a PIN code to protect the user directory, the data in your directory can be modified or deleted by others. 5.2.2 Logging in a user Press the [LOG IN/OUT] menu button above the screen. A list of all users appears. FlexCycler 2 Edition 11/2012 23

Operation Select a user with the arrow keys or enter the initials. The cursor automatically jumps to the field that begins with the corresponding letters. Press the [LOG IN] softkey and enter your PIN code. If you are not using a PIN code for the user directory, the following screen will not appear. Confirm the PIN number with the [PIN-CODE OK] key. The current user is displayed: To continue programming or to start a program, press one of the menu buttons above the display. 24 Edition 11/2012 FlexCycler 2

Operation 5.2.3 Creating a new user directory Press the [LOG IN/OUT] menu button above the screen. To create a new user, press the [NEW USER] softkey. Enter a name and the 2-3-digit initials. Enter a personal PIN code. Repeat the entry. Note: The PIN code is optional. If you do not wish to protect your user directory by a PIN code, leave the "ENTER PIN" field empty and continue with [USER-DATA OK]. Note: Without a PIN code to protect the user directory, the data in your directory can be modified or deleted by others. Note: You can change your PIN code at any time in the SYSTEM menu (see section "Changing the PIN code" p. 57). 5.2.4 Logging in as a supervisor The supervisor (administrator) can make global changes to the device settings. This includes the boot language of the system and deleting user directories. Press the [LOG IN/OUT] menu button above the screen to log in as a supervisor. Press the [SUPERVISOR LOG IN] softkey and enter the supervisor PIN code. FlexCycler 2 Edition 11/2012 25

Operation The factory setting for the supervisor PIN code is 000 000 0 (entered without spaces). 5.2.5 Deleting a user directory Users are able to delete their own user directories in the SYSTEM menu (see section "Deleting a user directory" p. 57). In addition, the supervisor can delete any user directory, regardless of whether the directory is PIN code-protected or not. To do so, log in as a supervisor (see section "Logging in as a supervisor" p. 25) and select the "DELETE user" option in the SYSTEM menu (see section "Deleting a user directory (user account)" p. 67). Select the user whose directory you wish to delete and press the [OK] key to confirm. This is particularly useful if an employee has left the group without deleting his user directory and programs. Note: Deleting a user directory also automatically deletes all programs in the corresponding directory. Make sure that you copy all important programs you would like to keep into another directory before deleting the user directory. Note: Deleting a user directory cannot be undone. 26 Edition 11/2012 FlexCycler 2

Operation 5.3 Creating a new program 5.3.1 Creating a program Log in to your user directory (see section "Logging in a user" p. 23). Press the [PROGRAM] menu button above the screen to get into the PROGRAM menu of the FlexCycler 2. Press the [NEW PROGRAM] softkey. The programming table opens. 5.3.2 Selecting the block type The FlexCycler 2 automatically detects the installed block module during the start-up process (block format, with/without gradient function). When creating a new program this module is accepted as default. It is also possible to create a program for a block format other than the currently installed block. In this case, the block type can be selected via the [BLOCK TYPE] softkey. FlexCycler 2 Edition 11/2012 27

Operation Select the correct block type from the list and press [SELECT]. A "G" stands for gradientcapable blocks. In this example the standard block type is the "TWIN 48G". 5.3.3 Heated lid settings With the standard settings the lid is preheated to the programmed temperature before the actual program starts. It is also possible to deactivate preheating the lid (PREHEAT LID: OFF). In this case the program already starts whilst the lid is still heating up. Press the [PREHEAT ON/OFF], softkey to switch between the two settings. Confirm the entry with [ENTER] or press the [RIGHT] arrow key to jump to the next entry field. Note: The recommended setting is "PREHEAT ON" Note: In the "PREHEAT ON" mode the block is kept constantly at 25 C whilst the lid heats up. After the heated lid has reached the target temperature, a 40 s equilibration period follows before the program is started. During this phase, a homogeneous temperature cushion forms between the samples. This results in a better temperature uniformity between the samples. 28 Edition 11/2012 FlexCycler 2

Operation 5.3.4 Setting the lid temperature Thanks to the High Performance Smart Lid technology much lower lid temperatures can be used than was common in the past. The advantage here is that a local overheating of the samples in the center of the block by heat radiation from above is prevented. The homogeneous heat cushion that forms in the High Performance Smart Lid leads to a considerably higher temperature uniformity between the samples compared to conventional heated lids. Enter the desired lid temperature and confirm with [ENTER]. Note: The recommended lid temperature for typical PCR reactions is 99.0 C. 5.3.5 Entering the temperature and hold time for a step All steps of a program are entered in a well-structured table. Numbers can be entered via the number pad. To navigate within the table use the arrow keys and the [ENTER], [BACK] and [CLEAR] keys. Number pad for entering numeric values. Use the arrow keys to navigate in the table. Every entry must be confirmed with [ENTER]. The cursor automatically jumps to the next field. You can also adopt an entry with the [RIGHT] arrow key. With [BACK] the cursor jumps back to the previous field. Within one row you can also use the [LEFT] arrow key for that purpose. FlexCycler 2 Edition 11/2012 29

Operation Use the [CLEAR] key to undo wrong entries. The previously entered value is removed. Alternatively, entries can be overwritten if the cursor is in the corresponding field. Enter the temperature for the first step and press the [RIGHT] arrow key or [ENTER]. The cursor jumps into the next column. Here, enter the hold time for this step. Note: When entering the time for a step minutes and seconds can but do not have to be separated by a period. The numbers can also be entered without a separating period. Example: To enter a time for a step of 2 min and 30 s, type "2", "3", "0". Depending on the column heading the time is shown as 02:30 (m:s). Note: Minutes can be entered faster by adding a period after the number. Example: Enter "2", " " to program a hold time of 2 minutes. 5.3.6 Defining a loop A loop is generally defined by two parameters: The target for the return ("GOTO") and the number of returns ("LOOPS"). The parameters for a loop are entered in the last step of the loop. Place the cursor on the last step of the loop to be programmed (in the example: step 4). Enter the target for the loop in the "GOTO" row (in the example: step 2). 30 Edition 11/2012 FlexCycler 2

Operation Enter the number of returns in the "LOOPS" row and confirm with [ENTER]. The loop is indicated by a bracket on the left side of the table. Note: The total number of loops displayed in the bracket is the result of the number of returns plus 1. 5.3.7 Cooling the samples after the run The samples can be cooled at a constant temperature after the program has ended. Enter the desired target temperature and the time "0" in the corresponding step. The software interprets the value "0" as an entry for the pause step and displays it as such (see also section "Programming a pause" p. 31). When this step is reached, the device keeps the programmed temperature for an unspecified time. Note: To increase the service life of the Peltier elements we recommend sample incubation at a temperature of 15 C after the program has ended. The frequently used temperature of 4 C shortens the life of the Peltier elements of the device. 5.3.8 Programming a pause If you want the FlexCycler 2 to hold a specific temperature over an undefined period of time, enter the value "0" as the hold time. After confirming the entry with [ENTER] the word "PAUSE" is displayed. The pause function is used, for example, for PCR protocols with manual hot start (open the heated lid, insert the samples, press [CONTINUE] to go to the next step of the program) or for cooling the samples after the reaction is over (see section "Cooling the samples after the run" p. 31). IMPORTANT If you are using the pause function make sure that you stop the program properly in the last step after taking out the samples (see section "Ending a program in pause" p. 51). If the device is shut down without stopping the program it will interpret this as a power failure and continue with the pause step when it is started again. FlexCycler 2 Edition 11/2012 31

Operation 5.3.9 Saving the program Press the [SAVE/SAVE AS] key. The first free program number is selected automatically. If you wish to save the program under a different number, use the arrow keys to select the number or enter it with the numeric keys. Press the [EDIT NAME] softkey and enter a name for the program. Confirm the name with [OK] and save the program with the [SAVE] softkey. 32 Edition 11/2012 FlexCycler 2

Operation The program number, name and date are shown in the directory tree. 5.3.10 Programming the temperature gradient Gradients can be programmed directly in the program table or with the [EDIT GRADIENT] softkey. The easiest way of defining a gradient is to enter the temperatures of the left and right block side separated by a dash (example 40 60). Acknowledge with [ENTER]. Alternatively, you can move the cursor to the " C" column and press the [EDIT GRADIENT] softkey. FlexCycler 2 twin 48G FlexCycler 2 96G Here, you can enter the temperature for column 1 and column 8 of the block (twin 48G) or for column 1 and 12 (mono 96G). As soon as both entries have been confirmed with [ENTER], the temperatures in the individual rows are displayed as a bar chart. To return to the table, press the [EDIT TABLE] softkey. FlexCycler 2 Edition 11/2012 33

Operation The [UP] or [DOWN] arrow keys can be used to fine-adjust the gradient. Press the arrow keys to increase or reduce the entered temperature in 0.1 C steps. Note: Programs with gradient steps can only be started on blocks with gradient function. Please note that for twin block modules only the left block (block 1) features the gradient function. If you attempt to start a gradient program on a block without gradient function, a corresponding message appears: 5.3.11 Programming a linear gradient Press the [EDIT LINEAR GRADIENT] softkey. FlexCycler 2 twin 48 G FlexCycler 2 twin 96 G Enter the desired annealing temperature and define the temperature gaps "INCR." between the rows in the heating block. The entered annealing temperature corresponds to the temperature in row 6 for 96 well blocks and in row 4 for 48 well blocks. The temperatures for all other rows will be calculated automatically based on the increment that has been entered. 34 Edition 11/2012 FlexCycler 2

Operation FlexCycler 2 twin 48 G FlexCycler 2 twin 96 G 5.4 Setting program options Additional parameters, such as time and temperature increments, can be defined for every step and the heating and cooling rate can be adapted. You can find these parameters by moving the cursor all the way to the right in the programming table, to the column titled "OP- TION". An additional section of the table will open up: To get back to the programming table, move the cursor to the left. If any parameters have been changed under "Option", the table view will display an additional "+" sign in the "OP- TION" column. FlexCycler 2 Edition 11/2012 35

Operation 5.4.1 Setting the heating and cooling rate The average heating and cooling rate can be set in the ( C/s) column. Note: The value in the ( C/s) column defines the speed with which the current step is reached. This means that if heating (or cooling) should be adapted to a speed of 1 C/s between step 2 and step 3, a value of 1.0 must be entered in step 3. Note: If the speed is to be reduced for the whole program the heating or cooling rates of all steps must be adjusted. The configuration then also only applies to this specific program. 5.4.2 Changing the hold time in cycles Δt(s) To compensate for a loss in enzyme activity during longer protocols, the hold time of a specific step can be increased between cycles (within a loop). Enter the desired hold time increase in the Δt(s) column of the corresponding step. During each cycle the hold time will be increased by the value entered here. Note: Please make sure that the corresponding step is within a loop. Otherwise, an entry in the Δt(s) column will be without effect. Note: An entry in the Δt(s) column for increasing the hold time will influence the total runtime of the protocol. A program with many cycles and significant increases of the hold time takes considerably longer than the same program without a programmed increase would. 36 Edition 11/2012 FlexCycler 2

Operation 5.4.3 Changing the temperature in cycles ΔT( C) For some applications it makes sense to start with a high temperature and lower the temperature from cycle to cycle. This technique is also known as "touch down". To lower the temperature from cycle to cycle in a specific step, enter a negative temperature value into the ΔT( C) column. The temperature will change by the entered value with every cycle. Note: Make sure that the step is within a loop. Otherwise, an entry in the ΔT( C) column will be without effect. 5.5 Editing programs Programs can be edited in the tabular or graphical mode. You can switch between the two alternative modes with the softkeys. The graphical mode is particularly useful for the quick adjustment of existing programs. The programs can then be saved under a new name (see section "Editing programs in the graphical mode" p. 42). Note: It is not possible to change the active program during operation. All changes must be made before starting the program. The program must be saved for the changes to become effective. The only way to apply changes to a running program is the skip function for skipping steps (see section "Skipping a program step" p. 49 ). FlexCycler 2 Edition 11/2012 37

Operation 5.5.1 Inserting a step Move the cursor to the step in front of which you wish to insert a new step. Press the [INSERT/DELETE STEP] softkey. Press the [INSERT STEP] softkey. Confirm with [OK]. Enter the temperature and hold time for the new step. 38 Edition 11/2012 FlexCycler 2

Operation Note: In the example the step was inserted within a loop. The loop now has 4 steps. (The existing loop is automatically updated). 5.5.2 Deleting a step Move the cursor to the step to be deleted and press the [INSERT/DELETE STEP] softkey. Press the [DELETE STEP] softkey to delete the marked step. Confirm the choice with [OK]. The total number of steps is now reduced by one step. Note: In the example the step was inserted within a loop. The loop now only has 3 steps. (The existing loop is automatically updated). FlexCycler 2 Edition 11/2012 39

Operation 5.5.3 Copying the program Press the [PROGRAM] menu button and the [OPEN DIRECTORY] softkey. Move the cursor to the program to be copied. Press the [COPY PROGRAM] softkey. The next free memory space in the user folder is automatically selected for saving the copy. With the arrow keys or by entering the corresponding number on the number pad of the operating panel, any other location can be chosen. The program will be saved under a new program number for unique identification. If you wish to change the name press [EDIT NAME]. Enter the new name and confirm with [ENTER]. Note: You can also copy programs from PIN code-protected user directories to your own directory. To do so, log on to the device and open the corresponding user directory. Select the program to be copied and press [COPY PROGRAM]. The program will be copied from the other user directory to your own. 40 Edition 11/2012 FlexCycler 2

Operation 5.5.4 Deleting a program Select the program to be deleted. Press the [DELETE PROGRAM] softkey. Confirm with [OK]. The memory space is now free. Note: Only programs from a user's own directory or from non-pin code-protected directories can be deleted. Deleting programs cannot be undone. FlexCycler 2 Edition 11/2012 41

Operation 5.5.5 Editing programs in the graphical mode Programs are usually created in the table view, which permits the fast programming of new steps. The graphical mode also offers a schematic representation of the temperature profile. You can switch between the table view and the graphical mode with the [GRAPH] or [TABLE] softkey. Table view Graphical mode To change parameters in the graphical mode, please move the cursor to the corresponding field and enter the new value. After confirming the entry with [ENTER] the graphical mode will be updated. Note: For an ideal representation the temperature stages are distributed on the display with maximum distances. This means that the location of the stages does not correspond to the effective temperatures. Note: To define loops switch to the table view (press [EDIT TABLE]). Note: To insert or delete steps switch to the table view (select [EDIT TABLE]). Note: To change a gradient or to enter a new gradient press the [EDIT GRADIENT] softkey (see section "Programming the temperature gradient" p. 33). Alternatively, you can switch to the table view for programming a gradient. The function for programming gradients is only available if a gradient-capable block is inserted at the device or if a gradient-capable block type is selected in the programming table (see section "Selecting the block type" p. 27). 42 Edition 11/2012 FlexCycler 2

Operation 5.6 Starting a program On the user interface of the FlexCycler 2 there is a [START] key for starting programs (see section "User interface" p. 19). 5.6.1 Selecting a program for the start Any user can start any program, including programs from protected directories of other users. Press the [PROGRAM] menu button above the display. Open the corresponding directory [OPEN DIRECTORY] and select the program to be started. For devices with Monoblock the selected program can be started directly by pressing the [START] key on the user interface Note: When starting programs with the appendix "GRAD" and that include a gradient step, a temporary adjustment to the currently used block type may be necessary (the saved program will not be modified). The following table provides an overview of the possible adjustments made by the FlexCycler 2 : Cause Example Adjustment Maximum gradient range for the selected block is exceeded. A program for the 96 well gradient block type with a programmed temperature range > 20 C is to be started on a 48 well gradient block and therefore exceeds the block's maximum gradient range. The temperature range of the gradient will be reduced based on the average temperature of the maximum permissible value. If the average temperature of the programmed gradient is, for example, 60 C and the temperature range is ± 15 C, it will be reduced to 60 C ± 10 C. FlexCycler 2 Edition 11/2012 43

Operation A block without gradient function has been selected. A program for the 96 well gradient block type or 48 well gradient block type is started on a block without gradient function. The gradient will be deleted and the average temperature will be used for this step. If the average temperature of the programmed gradient is, for example, 60 C, this value will be used for the step. If adjustments are necessary to start a program, the following message will appear briefly (5s): The message describes the reason for the adjustment and for which block the started program is being adjusted. 5.6.2 User-specific quick-start of programs The FlexCycler 2 records the last five started or edited programs for each user individually. With the quick-start function, these programs can be selected conveniently and started immediately. Press the [BLOCK] menu button above the display. Monoblock Twinblock 44 Edition 11/2012 FlexCycler 2

Operation Twinblock only: Select one or both blocks. Select one of the programs with the cursor and press the [START] key on the control unit. Note: When starting programs with the appendix "GRAD" and that include a gradient step, a temporary adjustment to the currently used block type may be necessary. The saved program will not be modified. See also section "Selecting a program for the start" p. 43. 5.6.3 Program preview Before starting a program, you can check it in a preview mode. Press the [VIEW PROGRAM] softkey (see section "User-specific quick-start of programs" p. 44). Note: In this menu it is not possible to edit the program. The [LATEST PROGRAM LIST] function will take you back to the list of programs last used by the currently logged-in user (see section "User-specific quick-start of programs" p. 44 ). The FlexCycler 2 provides the option for both tabular and graphical program preview. Press [VIEW GRAPH] or [VIEW TABLE], to switch between both modes. Table view Graphical mode If the program has a programmed gradient, it is also possible to open a preview showing the shape of the gradient. Press [VIEW GRADIENT] to change to the gradient preview. FlexCycler 2 Edition 11/2012 45

Operation FlexCycler 2 twin 48 G FlexCycler 2 twin 96 G 5.6.4 Display during a run After the program has been started, the following screen will be shown: Monoblock Twinblock The status of a block can be free, active or paused. The user who started the program and the calculated remaining runtime are displayed. If you are using a Twinblock device, the status of both blocks will be shown. Aside from the remaining runtime, there is other information that can be called up for a running program. The software offers the following basic view options: [TIME] [GRAPH] [TABLE] [GRADIENT] Remaining runtime display Graphical representation of the program Tabular representation of the program Gradient display (only possible in gradient steps) You can switch between the different views with [SHOW TIME], [SHOW TABLE], [SHOW GRAPH] and [SHOW GRADIENT]. The corresponding keys are assigned several functions - pressing a key several times will switch between these functions. For Twinblock devices, first select a block with the softkeys [BLOCK 1] or [BLOCK 2] and press one of the [SHOW TIME], [SHOW TA- BLE], [SHOW GRAPH] or [SHOW GRADIENT] keys. 46 Edition 11/2012 FlexCycler 2

Operation Remaining time Graphical representation Tabular representation Gradient view Twinblock only: To display the remaining time, press [OVERVIEW TIME] and to display the temperature of both blocks press [OVERVIEW TEMPERATUR.]. Overview time Overview temperature Note: The screen always shows the average temperature of the block. This also applies for gradient steps. FlexCycler 2 Edition 11/2012 47

Operation 5.6.5 Pausing the program Twinblock only: To pause a program in the current step select the corresponding block with the softkeys [BLOCK 1] or [BLOCK 2]. Press [PAUSE BLOCK / SKIP STEP]. Press [BLOCK PAUSE]. The block status displayed is now "PAUSE". It is shown alternating with the elapsed time since pause has started. 48 Edition 11/2012 FlexCycler 2

Operation 5.6.6 Continuing the program To continue the program and end the pause press the [CONTINUE] softkey. 5.6.7 Skipping a program step Twinblock only: To skip a program step select the corresponding block with the softkeys [BLOCK 1] or [BLOCK 2]. Press [PAUSE BLOCK / SKIP STEP]. Press [SKIP STEP]. FlexCycler 2 Edition 11/2012 49

Operation The current step is skipped. The program continues with the next step. 5.7 Stop the program To stop an active program press the [STOP] key on the control unit. Twinblock only: To stop a program step select the corresponding block with the softkeys [STOP BLOCK 1], [STOP BLOCK 2] or [STOP ALL BLOCKS]. Confirm stopping the program with [OK]. 50 Edition 11/2012 FlexCycler 2

Operation 5.7.1 Stopping a program in pause If a program is stopped during a pause (for example when incubating the samples at room temperature) this program should still be stopped properly before switching off the FlexCycler 2. Note: If the FlexCycler 2 is switched off during pause mode without stopping the program first, the software registers this as a power failure and saves the event to the run log files and error reports. 5.8 USB functions The USB connection on the front of the device (see section "Front view" p. 15) can be used to transfer programs with a USB stick. 5.8.1 Connecting the USB stick with the thermal cycler Plug the USB stick into the intended connection at the front of the device. If no USB stick is connected to the FlexCycler2 the message "NO DISKDRIVE FOUND" appears if the attempt is made to read memory content. Note: The maximum memory capacity of the USB stick should not exceed 2GB. FlexCycler 2 Edition 11/2012 51

Operation 5.8.2 Opening the USB folder Log in to your personal user directory and go to the PROGRAM menu (see section "PROGRAM menu" p. 22). Use the [UP] and [DOWN], arrow keys to switch to the "USB DRIVE" directory. After selecting the USB drive the memory content of the USB stick is read automatically and the message "READING FILE LIST" appears. After the memory has been read a list of the programs on the USB stick is displayed. 5.8.3 Deleting a program in the USB folder To delete a program on the USB stick press [DELETE PROGRAM] (see section "Opening the USB folder" p. 52). In the next window, confirm the selection with [OK] or press [CANCEL] to cancel deleting. 52 Edition 11/2012 FlexCycler 2

Operation Note: Deleted programs cannot be restored. 5.8.4 Copying the program from the USB folder To copy a program select the program from the list and press [COPY PROGRAM] (see section "Opening the USB folder" p. 52). Select a memory space in your user directory and press [SAVE COPY]. To stop the copying process press [CANCEL]. The name of the program to be copied can be changed before saving. Press the [EDIT NAME] softkey and enter a name for the program. Confirm your entry with [OK] or press [CANCEL] to cancel the modification of the name. FlexCycler 2 Edition 11/2012 53

Operation 5.8.5 Copying a program to the USB folder To copy a program to the USB stick, select the user directory and press [COPY PROGRAM]. In the next window, press [SAVE COPY TO USB]. The system will display a message screen on the copying progress. After copying is complete, one program has been added to the program list on the USB stick. Note: Name changes are not adopted when copying programs to the USB stick. If you used the [EDIT NAME] function and entered a new name this change will not be adopted with the [COPY TO USB] function. Save the program in you personal user directory under a new name before you execute [SAVE COPY TO USB]. 54 Edition 11/2012 FlexCycler 2

Operation 5.8.6 Maximum number of programs in the USB folder The maximum number of programs on the USB stick is 48. If this limit is exceeded, a corresponding message is shown. FlexCycler 2 Edition 11/2012 55

System settings 6 System settings To change the basic FlexCycler 2 settings press the [SYSTEM] menu button above the screen (see section "User interface" p. 19). The main window of the SYSTEM menu opens. Use the cursor keys to select a menu item and press [SELECT]. 6.1 User configuration This is an option for using user-specific parameters. Select a menu item with the cursor keys and press [SELECT] or [OVERVIEW] to return to the main window. 6.1.1 Setting the language The language can be selected individually for each user and will be saved for the user directory as additional information. The currently available languages are German and English. Note: The language for the display during boot-up (before a user is logged in) can be set by the supervisor (see section "Setting the boot language" p. 68). 56 Edition 11/2012 FlexCycler 2

System settings 6.1.2 Changing the PIN code With this option you can change the current user's PIN code. First, enter your current PIN code, then enter the new PIN code and repeat the entry. Confirm the entries with [PIN OK]. Note: If you have forgotten your PIN code, you can ask the administrator to reset it (see section "Changing a user's PIN code" p. 66). 6.1.3 Deleting a user directory Users can delete their own user directories if they are logged in to the thermal cycler. Important: Deleting a user directory deletes all programms as well. Always make sure to copy all important programs to a different directory before deleting a user directory. Note: The information from deleted user directories and programs cannot be restored! FlexCycler 2 Edition 11/2012 57

System settings 6.2 System configuration In this menu you can set the beep, screen contrast, date and time and the screen saver. The menu also has a function for memory defragmentation. Use the cursor keys to select a menu item and press [SELECT] or [OVERVIEW] to return to the main window of the SYSTEM menu. 6.2.1 Setting the acoustic signal Switch the beep to OFF/ON and confirm with the [SAVE] softkey. The beep sounds if the program is paused or stopped. The beep can be stopped by pressing any key (except for the [STOP] key on the control unit). The default setting is beep OFF [DEFAULT BEEP]. 58 Edition 11/2012 FlexCycler 2

System settings 6.2.2 Setting the contrast The screen contrast can be set individually for each user. You can move the bar for changing the contrast with the arrow keys. Accept the setting with [SAVE CONTRAST]. The default setting for the contrast is the middle position [DEFAULT CONTRAST]. 6.2.3 Setting the date and time Set the current date and time and confirm with [SET DATE AND TIME]. 6.2.4 Defragmenting the memory As the memory gets fuller, programs are deleted and new programs created, new data is stored fragmented in the memory over time. This may reduce the access speed to the memory and cause the software to react slower to user commands. By defragmenting the memory the saved information is sorted and stored in a sequence that allows faster access to the program functions. FlexCycler 2 Edition 11/2012 59

System settings Confirm your selection with [OK] and wait for the defragmentation process. 6.2.5 Setting the screen saver Set the screen saver to OFF or ON and adjust the screen saver wait time as required. Accept the settings with [SAVE]. The default setting is screen saver ON with a wait time of 30 minutes [DEFAULT]. 6.3 System information In this menu you can access information on the device and run log files of the latest five runs. The different types of information that can be accessed are described in the following sections. Use the cursor keys to select a menu item and press [SELECT] or [OVERVIEW] to return to the main window of the SYSTEM menu. 60 Edition 11/2012 FlexCycler 2

System settings 6.3.1 System information Under this menu item information on the system, such as the serial number, block type and current software version of the device is displayed. System info Hardware info In addition, the software version numbers of several hardware components can be accessed via the [HARDWARE INFO] function. Press [SYSTEM INFO] or [HARDWARE INFO] to switch between the two windows or press [OVERVIEW] to return to the main menu. 6.3.2 Viewing/saving the log files from the last nine runs During each run, information is protocolled by the thermal cycler. The log files of the last nine runs can be called up in this menu. Select a program from the list using the cursor keys and press [VIEW RUN-LOGFILE]. To save an individual run log file on a USB stick that is connected to the FlexCycler 2 press [RUN- LOGFILE TO USB]. The [ALL LOGFILES TO USB] function can be used to export all saved run log files at once. The run log file saves several sets of information that are relevant for the last run. To view the steps of the used program press [VIEW PROGRAM]. To display an overview of all messages for this run press [VIEW MESSAGES]. To export the selected run log files to a USB stick press [SAVE LOGFILE TO USB]. FlexCycler 2 Edition 11/2012 61

System settings The messages are displayed sorted by time and date. If a power failure exceeds 30 minutes a pause step at 4 C (freeze step) is started automatically as soon as the power returns. Press [VIEW FREEZE PROG], to display the freeze step. 6.4 SERVICE menu This menu provides access to several service functions of the instrument. 6.4.1 Displaying log files of the "Power-on" self test Every time the FlexCycler 2 is switched on, it performs a self test. The results of the test are saved and can be called up. 62 Edition 11/2012 FlexCycler 2

System settings 6.4.2 Performing an extended self test In addition to the automatic power-on self test, you can perform an extended device test. During the extended self test, the Flex Cycler 2 checks the parameters of different components and functions and saves the results in a log file. The test therefore offers the user important and useful information on whether the device is operating within specifications. To initiate an extended self test, press [EXECUTE SELFTEST]. The extended self test takes approx. 30 minutes. During the test, no program can run on the device. Within specific intervals, the device displays a message prompting the user to perform an extended self test (message 729): You can confirm that you have read the message by pressing [QUIT]. If the FlexCycler 2 is switched off without having performed the extended self test, the message is displayed again the next time the device is switched on. To document that the device is working properly, we recommend performing the extended self test at regular intervals. FlexCycler 2 Edition 11/2012 63

System settings 6.4.3 Displaying log files of the extended self test The results of the extended self test are stored in the device. To call up the results of the test, access the log files in the menu under [VIEW LOGFILE OF EXTENDED SELFTEST] and open them by pressing [SELECT]. The next window summarizes the current status of all important components. Press [MORE RESULTS] to display data on the run time of the device. Press [LIST LOGFILES], to return to the log files selection list. 64 Edition 11/2012 FlexCycler 2

System settings 6.4.4 Displaying the error history This menu shows the software and hardware error history. Note: Serious errors, that would impair the function of the device, are displayed as messages on the user interface and require user interaction. 6.4.5 Creating an info file for Analytik Jena Service This function creates an info file with technical details that serves as a remote diagnosis tool for Analytik Jena Service. Service info files can be transferred to a USB stick that is connected to the FlexCycler 2 thermal cycler or to a PC. To save the files to a USB stick select [SINF- File to USB]. To save the files to a computer, connect the FlexCycler 2 to a computer with a serial cable (full handshaking mode) and start the hyper terminal. Enter "SINF" into the input mask of the hyper terminal to start the file transfer. Send the SINF (service info file) to Analytik Jena Service by email (see section "Contacting Analytik Jena" p. 66). FlexCycler 2 Edition 11/2012 65

System settings 6.5 Contacting Analytik Jena This screen shows the Analytik Jena contact information. 6.6 Supervisor system settings The administrator (supervisor) has access to a special system menu with some special functions. 6.6.1 Changing a user's PIN code The supervisor can change the PIN code of every user directory. To do so, select the user directory from the selection list and press [SELECT USER]. Enter the new PIN code and repeat the code. Press [PIN OK]. 66 Edition 11/2012 FlexCycler 2

System settings 6.6.2 Deleting a user directory (user account) The supervisor can delete user directories regardless of whether these are PIN code protected or not. Select the correct user directory from the list and press [SELECT USER]. Confirm deleting the directory with [OK]. The directory and the programs in it are deleted. 6.6.3 Changing the supervisor PIN code The supervisor account is PIN code-protected. The supervisor PIN code is originally set to 0000000. Enter a new PIN code and repeat the entry. Confirm your entry with [PIN OK]. FlexCycler 2 Edition 11/2012 67

System settings Note: Write down the supervisor PIN code and keep the note at a safe place. If the supervisor PIN code has been lost, it can only be reset by the Analytik Jena Service or an authorized Analytic Jena dealer. 6.6.4 Setting the boot language The language for the display during boot-up can be set by the supervisor. Use the arrow keys to select the language and press [SELECT LANGUAGE]. The default language is English [DEFAULT LANGUAGE]. 6.6.5 Deleting all user directories (user accounts) The supervisor can delete all user directories and the programs in them at once. Confirm your selection in the security prompt by pressing [OK] to delete all directories. Note: The information from deleted user directories and programs cannot be restored! 68 Edition 11/2012 FlexCycler 2

Quick reference guide 7 Quick reference guide Safety instructions Do not open the device unless you have been authorized to do so! Before switching on the device, make sure that the voltage selector on the bottom is set to the right mains voltage (110 V, 115 V or 230 V, see section "Setting the operating voltage" p.13). The block and lid can reach high temperatures during operation. There is a risk of burning from touching these components. The samples are heated up quickly and may come to an explosive boil. Wear goggles when handling hot samples. Make sure that the lid is closed securely before starting the program! The use of oil between the samples and the block is NOT necessary to achieve an improved heat exchange. If you do wish to use oil, never use silicone oil but mineral oil instead. Ensure that the air vents are clear. Insufficient ventilation may cause the device to overheat. The FlexCycler 2 control panel Log in After the FlexCycler 2 has completed the initial self test, the LOGIN menu is displayed: Press [LOG IN] to log in as an existing user. Press [SUPERVISOR] to log in as supervisor. FlexCycler 2 Edition 11/2012 69

Quick reference guide Press [NEW USER] to create a new user. You can select the language for the control panel (German or English). The language setting is saved individually for each user. Press [SELECT LANGUAGE] to select the preferred language. To define the language for the screens during boot-up, log in as a supervisor and select "SET BOOT LANGUAGE" in the supervisor SYSTEM menu. Use the arrow keys to select the language and press [SAVE]. Creating, modifying and saving programs To create a new program or modify an existing program press the [PROGRAM] menu button. Select the desired user directory and press [OPEN DIRECTORY]. Press [NEW PROGRAM] or select an existing program and press [EDIT PROGRAM], to get to the programming window. Use the arrow keys to move through the programming table. The active field in which entries can be made is highlighted white. The assignment of the soft keys on the FlexCycler 2 control panel changes context depending. The FlexCycler 2 programming table To switch the heated lid on or off, activate the corresponding status view and press [PREHEAT ON/OFF]. 70 Edition 11/2012 FlexCycler 2

Quick reference guide The lid temperature can be set in the status view under [-- C] in a range between 30 C and 99 C. Enter a temperature [ C] and a time [m:s] into the programming table for every step in your PCR protocol. To program a temperature for a specific time enter "0" under [m:s] and press <ENTER>. The word "PAUSE" is displayed. Note: Minutes and seconds can be entered separated by a period or one after the other. For 2 minutes 30 seconds, for example, enter "2", " ", "3", "0" or "2", "3", "0". To insert or delete a program step move the cursor to the corresponding position in the program table and press [INSERT/DELETE STEP]. Select [DELETE STEP] or [INSERT STEP], to insert or delete a step. The position in which the step is inserted or deleted is highlighted. Confirm deleting or inserting the step with [OK] in the next window. To program a loop, specify a) which step the program is to go back to [GOTO] and b) how often he loop is to be repeated [LOOPS]. The loop is indicated with brackets on the left side of the program table. To program special functions such as temperature increments, time increments or heating and cooling rates, repeatedly press the [RIGHT] arrow key to open the options table. The new table will be displayed: Note: Set the temperature to 15 C instead of 4 C at the end of a run. This increases the life time of the instrument. To change the annealing temperature in a loop from step to step, enter a temperature increment with a negative or positive sign under T( C). To change the incubation time in a loop from step to step, enter a time increment with a negative or positive sign under T( C). The ramping rate (heating and cooling rate) is preset depending on the block type. To adjust the ramping rate, enter a value in steps of 0.1 C under [ C/S]. Only for blocks with gradient function: To define a gradient, enter a) two temperatures, separated by a minus sign, for the left and right side of the block under [ C] (e.g. 55-65 C) or b) press [GRADIENT] and then [EDIT LINEAR GRADIENT]. This activates the window for programming linear gradients: FlexCycler 2 Edition 11/2012 71

Quick reference guide Enter an annealing temperature for the center of the block (row 4 for 48 well blocks, row 6 for 96 well blocks). Enter the desired temperature increment under [INCREM.]. The corresponding temperature profile for each individual row is displayed. Press [SAVE/SAVE AS] to save a program. Tip: You can create a folder with general protocols. From this general folder the programs can easily be copied to the individual user directories. The program is saved at the first available memory location. To save a program in a specific memory location, use the [UP] or [DOWN] arrow keys or enter the number of the memory location. To change the name of a program press [EDIT NAME]. In the next window, enter a name and confirm your entry with [OK]. Starting, pausing and stopping programs The FlexCycler 2 saves the last 5 active or modified programs for each user. For quick access to one of these programs press the [BLOCK] menu button. In the next window, select one of the programs with the [UP] or [DOWN] arrow keys. Press the [START] button on the control unit to start the program. To start a program from the user directory, log in and press the [PROGRAM] menu button. In the next window, use the [UP] and [DOWN] arrow keys to select the corresponding user directory and program. Press the [START] button on the control unit to start the program. After the start of the program the following window appears: 72 Edition 11/2012 FlexCycler 2

Quick reference guide Monoblock Twinblock To pause an active program, press the [PAUSE BLOCK/SKIP STEP] key on the control unit. Twinblock only: To pause a program, select the corresponding block with the softkeys [BLOCK 1] or [BLOCK 2] and press the [PAUSE BLOCK/SKIP STEP] key. To continue a program that is on pause, press [CONTINUE] in the next screen. Twinblock only: To continue a program step select the corresponding block with the softkeys [BLOCK 1] or [BLOCK 2] and press [CONTINUE]. To stop an active program press the [STOP] key on the control unit. Twinblock only: To stop a program step select the corresponding block with the softkeys [STOP BLOCK 1], [STOP BLOCK 2] or [STOP ALL BLOCKS]. Programs for which a pause is programmed as the last step must also be stopped by the user. FlexCycler 2 Edition 11/2012 73

Troubleshooting 8 Troubleshooting 8.1 Slow heating and cooling The FlexCycler 2 is equipped with strong fans that remove the heat from the heat sink. The air supply to these fans is on the bottom of the device. Make sure that there is no dust or other obstruction (a piece of paper under the Flex Cycler 2, for example, may be sucked into the fan and restrict its functionality). Dirt should be frequently removed from the air supply of the fan with a vacuum cleaner or a brush. 8.2 Auto restart Running programs are continued in the same step that was active at the time of the power failure as soon as the power supply is back. At the same time, a message indicates that a power failure occurred during the run and when and in which step the program was restarted. Pressing [OK] closes the window with the message. After power failure of 30 minutes or longer the instrument starts a freeze step and keeps the temperature constant at 4 C. The user has to confirm that the started program shall be continued with the step during which the power supply failed. 8.3 Auto restart without recognizable cause Strong fluctuations in the voltage supply may cause the FlexCycler 2 to reboot. The FlexCycler 2 then reacts similar to a supply voltage failure and restarts the program with the step during which the supply voltage failed. At the same time, a message indicates that a power failure occurred during the run and when and in which step the program was restarted (see section "Auto restart" p. 74). To avoid strong fluctuations in the supply voltage, do not connect the FlexCycler 2 to a power outlet to which other powerful consumers (such as freezers, refrigerators or centrifuges) are connected. 74 Edition 11/2012 FlexCycler 2

Troubleshooting 8.4 Adjusting protocols of other thermal cyclers When transferring protocols from other, slower thermal cyclers it may be necessary to reduce the heating and cooling rate. To reduce heating and cooling rates see section "Setting the heating and cooling rate" p. 36. The hold times can be increased as well. 8.5 Loosening the lid wheel in case of blockage Note: If the lid is in the highest or lowest position, the friction clutch may be active in both directions. The clicking then occurs in both directions; the lid will not be lifted or lowered. In this case, use a pointed object to press on the metal pin on the top of the lid wheel. Hold the pin down and carefully turn the lid wheel one quarter turn. The metal pin bypasses the friction clutch and the blocked lid can be released. Lid blocked in the top position: 1. Press down the metal pin. 2. Carefully turn the lid wheel clockwise until it moves without resistance (no more clicking). 3. Release the pin. Lid blocked in the bottom position: 1. Press down the pin. 2. Carefully turn the lid wheel anticlockwise until it moves without resistance (no more clicking). 3. Release the pin. ATTENTION! Do not bypass the friction clutch to increase the contact pressure! When the friction clutch is active (= optimal contact pressure reached) do not, under any circumstances, press the metal pin again to increase the contact pressure further. This will damage the samples and the device. FlexCycler 2 Edition 11/2012 75

Maintenance and care 9 Maintenance and care 9.1 Cleaning and decontamination The housing of the FlexCycler 2 should be wiped with a soft cotton cloth from time to time. Never use aggressive detergent or solvents. The air vents on the bottom and the rear of the device should occasionally be cleaned with a vacuum cleaner. Additional regular maintenance is not required. CAUTION! Observe the safety regulations! Observe the relevant safety regulations when working with infectious or pathogenic material. The professional decontamination of radioactive contaminations depends on the type of substance used. Please contact your radiation protection officer on this matter and observe the relevant safety regulations! 9.2 Service and repair CAUTION! The FlexCycler 2 does not contain any parts that can be repaired by the operator. The housing of the FlexCycler 2 must only be opened by trained and authorized service personnel. The Analytik Jena Service department offers maintenance and temperature measurements for thermal cyclers. Analytik Jena recommends to service the device every year and to perform a temperature check and, if necessary, a calibration, every two years. 9.3 Firmware update Please contact Analytik Jena Service or your local dealer for instructions on how to load a new FlexCycler 2 firmware. 9.4 Spare parts Use original spare parts only. 76 Edition 11/2012 FlexCycler 2

Order numbers for FlexCycler2 and block modules 10 Order numbers for FlexCycler 2 and block modules 844-00060-2 FlexCycler 2 96, 230 V, English language manual 844-00060-3 FlexCycler 2 96, 230 V, German language manual 844-00060-4 FlexCycler 2 96, 115V, English language manual 844-00060-5 FlexCycler 2 96, 100V, English language manual 844-00061-2 FlexCycler 2 96G, 230 V, English language manual 844-00061-3 FlexCycler 2 96G, 230 V, German language manual 844-00061-4 FlexCycler 2 96G, 115V, English language manual 844-00061-5 FlexCycler 2 96G, 100V, English language manual 844-00062-2 FlexCycler 2 twin 48, 230 V, English language manual 844-00062-3 FlexCycler 2 twin 48, 230 V, German language manual 844-00062-4 FlexCycler 2 twin 48, 115V, English language manual 844-00062-5 FlexCycler 2 twin 48, 100V, English language manual 844-00063-2 FlexCycler 2 twin 48, 230 V, English language manual 844-00063-3 FlexCycler 2 twin 48G, 230 V, German language manual 844-00063-4 FlexCycler 2 twin 48, 115V, English language manual 844-00063-5 FlexCycler 2 twin 48, 100V, English language manual 844-00064-2 FlexCycler 2 twin 30, 230 V, English language manual 844-00064-3 FlexCycler 2 twin 30, 230 V, German language manual 844-00064-4 FlexCycler 2 twin 30, 115V, English language manual 844-00064-5 FlexCycler 2 twin 30, 100V, English language manual 844-00065-2 FlexCycler 2 twin combi, 230 V, English language manual 844-00065-3 FlexCycler 2 twin combi, 230 V, German language manual 844-00065-4 FlexCycler 2 twin combi, 115V, English language manual 844-00065-5 FlexCycler 2 twin combi, 100V, English language manual 844-00069-2 FlexCycler 2 basic unit The following exchangeable block modules are available for the FlexCycler 2 : Order number Model 844-60060-0 FlexCycler 2 block 96 844-60061-0 FlexCycler 2 block 96G 844-60062-0 FlexCycler 2 block twin 48 844-60063-0 FlexCycler 2 block twin48g 844-60064-0 FlexCycler 2 block twin 30 844-60065-0 FlexCycler 2 block twin combi FlexCycler 2 Edition 11/2012 77

Service 11 Service If you have any problems with the device please contact our Service department or your local Analytik Jena dealer: Analytik Jena AG Life Science Department Konrad-Zuse-Strasse 1 D-07745 Jena Germany Phone: +49(0)3641 77-94 11 Fax: +49(0)3641 77-76 77 76 Email: k.fritzsche@analytik-jena.de IMPORTANT Please observe the return information (see section 11.1) if you wish to return a device. 11.1 Device return information If your FlexCycler 2 is faulty, please proceed as follows: Please only send in devices that are really faulty! If a technical problem occurs, contact the Analytik Jena Service department first. If you need to send in your device, the Service will assign an RMA number (RMA = Return Material Announcement) which you will receive over the phone. This number must be attached to the device packaging in a place that is clearly visible. Devices submitted without an RMA will not be accepted by Analytik Jena. IMPORTANT: Please clean all device components from biologically hazardous, chemical and radioactive contamination. If a contaminated device arrives at Analytik Jena, we must refuse acceptance. The sender of the item may be liable for any damage caused by inadequate decontamination of the device. Complete the written decontamination declaration and attach the decontamination declaration to the outside of the shipment. You will receive the form for the decontamination declaration together with the service documentation. Only use the original box for the shipment (see section 11.2). If this is no longer available, please contact Analytik Jena or your local dealer. Please attach the warning note "CAUTION! SENSITIVE ELECTRONIC DEVICE!" to the packaging. Complete the service documentation fully and include it with the device. 78 Edition 11/2012 FlexCycler 2

Service 11.2 Packaging the thermal cycler For dispatch, Analytik Jena uses a specially designed packaging system where the device is held in place by two tear-proof plastic sheets. When packaging the FlexCycler 2 the device is placed on the plastic sheet of the lower insert element and secured between the two sheets by pressing on the upper insert element. ATTENTION! The FlexCycler 2 is only protected against transport damage if the packaging instructions are adhered to and the device is secured between the plastic sheets. Analytik Jena is not liable for transport damage due to incorrect packaging. Mount instrument floating in between the foils! FlexCycler 2 Edition 11/2012 79

Disposal 12 Disposal At the end of its service life, the FlexCycler 2 and all its electronic components must be disposed of as electronic waste in accordance with valid regulations. 80 Edition 11/2012 FlexCycler 2

Declaration of conformity 13 Declaration of conformity EU Konformitätserklärung EC - Declaration of Conformity Göttingen, November 2012 im Sinne der EG-Richtlinie über elektrische Betriebsmittel zur Verwendung innerhalb bestimmter Spannungsgrenzen 2006/95/EG following the EC directive about electrical equipment for use within certain limits of voltage 2006/95/EC und / and im Sinne der EG-Richtlinie für die elektromagnetische Verträglichkeit 2004/108/EG. following the EC directive about the electromagnetic compatibility 2004/108/EC. Hiermit erklären wir, dass folgende Thermal cycler: Herewith we declare that the following Thermal cyclers: Typen / types: FlexCycler 2 96, FlexCycler 2 96G, FlexCycler 2 twin 48, FlexCycler 2 twin 48G, FlexCycler 2 twin 30, FlexCycler 2 twin combi Best.-Nr. / Order No.: 844-00060, 844-00061, 844-00062, 844-00063, 844-00064, 844-00065 den grundlegenden Anforderungen der corresponds to the basic requirements of EG-Niederspannungsrichtlinie 2006/95/EG und der EC low voltage directive 2006/95/EC and the EG-Richtlinie über die elektromagnetische Verträglichkeit 2004/108/EG entsprechen. EC directive about the electromagnetic compatibility 2004/108/EC. Dr. Jürgen Otte Quality Manager For and behalf of Biometra GmbH Certificate number FS 68563 FlexCycler 2 Edition 11/2012 81

Warranty 14 Warranty This Analytik Jena device was produced and tested with the utmost care and subjected to a quality control before delivery. Analytik Jena guarantees that the device corresponds to the specifications described in this manual. This warranty includes material or processing defects and applies under the following conditions: This warranty applies 24 months from delivery by Analytik Jena to the customer. Without a written permission by Analytik Jena this warranty is not transferable to other persons. This warranty applies for this device and the included original accessories only. It is valid only if the device is operated in accordance with the instructions in this manual. Analytik Jena shall repair or replace every faulty device that is sent in. The warranty does not cover wear caused by unintended use, negligence or unauthorized modifications or misuse of any parts of the device. 82 Edition 11/2012 FlexCycler 2

Index 15 Index Auto restart 75 Block menu 21 Block type 27 Boot language 68 Contact 66 Contrast 59 Control unit 17 Date and time 59 Decontamination 77 Defragmentation 59 Edit program 37 Exchanging the block 16 Extended self test 63, 64 Firmware update 77 Gradient 33 linear gradient 34 Heated lid 17, 76 legal disclaimer 8 Log file 61, 62 Log in 20, 23 Maintenance 77 Menu button 19 Operating voltage 13 Pause 48 PIN code 57, 67 Program copy 40 create 27 delete 41 delete step 39 end 50 gradient 33 graphical programming 42 heating and cooling rate 36 hold time 29, 36 insert step 38 linear gradient 34 loop 30 pause 31, 48 remaining time 46 save 32 skip step 49 start 43 tabular programming 42 temperature 29 Program menu 22 Quickstart 21 Quick-start 44 Repair 77 Safety instructions 10 Screen saver 60 Self test 63, 64 Service 77 Service info file 65 Setting the language 56 Signal tone 58 Softkey 20 Start 43 Stop 50 Supervisor 25, 66 System information 60 System menu 22 System settings 56, 58 Technical specifications 7 USB copy program 53, 54 delete program 52 folder 52 max. number of programs 55 USB functions 51 User configuration 56 User directory create 25 delete 26, 57, 68 Warranty 83 FlexCycler 2 Edition 11/2012 83