CURRICULUM VITAE. Work experience

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1 CURRICULUM VITAE Name Carlos Manuel Mendes Novo Date of birth 1st of January 1952 Nationality Portuguese Academic Qualification Chemistry Degree at Lisbon University, Portugal, Public dissertation, equivalent to Master, INETI, Portugal, 1991 PhD in Biochemistry at King s College London, Work experience Secondary school teacher of Physics, Chemistry and Mathematics Laboratory Technician at the Medical Sciences Faculty of Lisbon (Immunology Department) Research probationer at National Laboratory of Industrial Technology and Engineering (LNETI) Research Assistant at National Institute of Industrial Technology and Engineering (INETI) (since May 18th) Auxiliary researcher at INETI (since September 1 st ) Unit Director at Biotechnology Department of INETI. Since 1991 Supervision of PhD, BsC and University Final Course thesis in Protein and Monoclonal antibody fields. Since 1989 Teaching and organization of practical courses in Protein and Monoclonal antibody fields Tel: + (351) Fax: + (351) [email protected] 1. Most relevant scientific papers 1. Evaluation of the presence of aspartic proteases from Centaurea calcitrapa during seed germination. Salvador, S.M., Novo, C., Domingos, A. Enzyme and Microbiol Biotechnology 2005 (in press). 2. Sequences of N termini of coat protein of Portuguese Zucchini yellow mosaic virus isolates and of an epitope recognized by a monoclonal antibody. Cardoso, F., Armada, A., Fonseca, A.M., Teixeira, M., Sequeira, J., Clemente, A, Sequeira, O., Novo, C., J. Plant Pathology, 2005 (in press). 3. Searching for nitrile hydratase using the Consensus Degenerate Hybrid Oligonucleotide Primers strategy. Lourenço, P.M.L., Almeida, T., Mendonça. D., Simões, F., Novo, C. (2004) J. Basic. Microbiol. 44, Aspartic proteases from the rodent parasite Plasmodium chabaudi, a suitable model for Plasmodium. falciparum plasmepsins. Martins, T.M., Novo, C., do Rosário, V.E., Domingos, A. (2003) Acta Tropica 89, Burkholderia genome analysis reveals new enzymes belonging to the nitrilase superfamily. The amidase of Burkholderia cepacia (hospital isolate) Novo, C., Tata, R., Cemente, A., Brown, P.R. (2003) Int. J. Biol. Macromol. 33, Support for a 3D structure predicting a Cys Glu Lys catalytic triad for Pseudomonas aeruginosa amidase comes from site directed mutagenesis and mutations altering substrate specificity. Novo, C., Farnaud, S., Clemente, A., Brown, P.R. (2002) Biochem. J. 365,

2 7. A monoclonal antibody specific for the amidase of Pseudomonas aeruginosa. Novo, C., Karmali, A., Clemente, A., Brown, P.R. (2001) Hybridoma 20, Production and characterization of a mouse monoclonal antibody against the Gag p26 protein of Human Immunodeficiency virus type 2. Marcelino, J., Novo, C., Picotez, F., Moniz Pereira, J., Clemente, A., Taveira, N. (2001) AIDS Research and Human Retroviruses. 17, Purification and characterization of monoclonal antibodies against the free α subunit of Human chorionic gonadotrophin, Novo, C., Domingos, A., Karmali, A. (2001) Molecular Biotech. 17, Primary structure deduction and molecular modelling from a cdna of a cellobiohydrolaselike protein from the white rot fungus Coriolus versicolor Novo, C., Simões, F., Mendonça, D., Matos, J., Clemente, A. (2001) IJBM, 28, Pseudomonas aeruginosa aliphatic amidase is related to the nitrilase/cyanide hydratase enzyme family and Cys 166 is predicted to be the active site nucleophile of the catalytic mechanism. Novo, C., Tata, R., Clemente, A., Brown, P.R. (1995) FEBS Lett, 367, Human α fetoprotein: isolation and production of monoclonal antibodies, Karmali, A., Novo, C., (1990) Biochimie, 72, Altered fatty acids membrane composition modifies lymphocyte localization in vivo, Novo, C., Fonseca, E., Freitas, A. (1987) Cell Immunol, 106, Oral Communications (last 5 years) 1. Serine proteases involved in the mosquito immune response. Domingos, A., Gabriel, A., Laires, R.S., do Rosério, V.E., Almeida, P.G., Novo, C., Silveira, H. 2 nd Annual Meeting of Portuguese Proteomic network Procura, Novembro, Lisboa, New monoclonal antibodies developed against dsrna for diagnostic in grapevine and fruit trees viruses. Cardoso, F., Baptista, c., Sousa, E., Novo, C. 14 th Meeting of the International Council for the study of virus and virus like diseases of the grapevine (ICVG), p 191, Locorotondo (Bari), Italy, Amidase/Nitrile hydratase operon from Agrobacterium tumefaciens. Almeida, T., Lourenço, PML, Mendonça, D., Simões, F., Novo, C. 6º International Symposium on Biocatalysis and Biotransformations, Cost D25 Workshop, p.342, Olomouc, Check Republik, Murine monoclonal antibodies against envelepe of HIV 2 primary isolate from Guinea Bissau. J. Marcelino, S. Silva, H. Barroso, C. Novo, N. Taveira. 1º Congresso da Sociedade Portuguesa de Ciências Farmacêuticas, O 15, Lisboa, Expression of the Plasmodium chabaudi haemoglobinase plasmepsin 1. T.M. Martins, D. Wyatt, C. Novo, V.E. do Rosário, C. Berry, A. Domingos.XIII Congresso Nacional da Soc. Port. de Bioquímica, S10(CS1) p 111, Lisboa, The use of a phage display library for the determination of the epitopes recognized by monoclonal antibody against plant virus ZYMV, A. Fonseca, A. Armada, F. Cardoso, C. Novo, Micro2001, CO18, Congresso Nacional de Microbiologia, Póvoa do Varzim, Posters (last 5 years) 1. A sensitive and specific elisa assay for the detection of the antibody response to the HIV 2 envelope glycoprotein in single and dual HIV infections. J.M. Marcelino, H. Barroso, F. Gonçalves, S.M. Silva, C. Novo, P. Gomes, R. Camacho, N. Taveira. 3rd IAS Conference on HIV Pathogenesis and treatment 2005, Rio de Janeiro.

3 2. Monoclonal antibodies with catalytic activity towards viral dsrna. R. Madeira, F. Cardoso, C. Novo. XIV Congresso Nacional de Bioquímica, Vilamoura, Protein disulfide isomerase as target for chemotherapy. Isolation and sequencing of the protein disulfide isomerase from Plasmodium chabaudi. S. Prata, T. M. Martins, A. Domingos, V. E. do Rosário, C. Novo. PJ.46, XIV Congresso Nacional de Bioquímica, Vilamoural Plasmodium proteases as targets for malaria therapy. T. Martins, R. Caldeira, J. Pombo, A. Afonso, P. Cravo, V. do Rosário, C. Novo, C. Berry, A. Domingos. 1st Annual meeting fo the COST Action 857, P 40, Lisboa, Babesia proteases as targets for veterinary therapy. T. Martins, S. Teixeira, R. Caldeira, A. Leitão, C. Novo, A. Domingos. 1st Annual meeting fo the COST Action 857, P 41 Lisboa, Detection of an antigen in serum of mice infected with Plasmodium chabaudi. A. Armada, A.T. Fonseca, A. Puaty, V do Rosário, C. Novo. 1st Annual meeting fo the COST Action 857, P 28, Lisboa, The role of neutralizing antibodies in the disease evolution of children infected by HIV 2 transmitting mother. J. Marcelino, R. Antunes, M.A. Lourenço, L. Rosado, C. Novo, N. Taveira. Keystone Symposia, Alberta, Canadá Detecção do depósito amilóide por cintigrafia com o componente P na polineuropatia amiloidótica familiar tipo português: purificação do componente P. A. Lopes, F. Teixeira e Costa, F. Carvalho, Mª João Galvão, C. Novo. X Congresso Nacional de Biotecnologia Biotec2003, Lisboa. 9. Detection of an high molecular weight circulating antigen from Plasmodium chabaudi. A. Pereira, A. Armada, V.E. Rosário, C. Novo. X Congresso Nacional de Biotecnologia Biotec2003, Lisboa. 10. Degradation of haemoglobin by recombinant aspartic proteinase from Plasmodium chabaudi, plasmepsin. J. L. Pombo, T. M. Martins, R. A. Caldeira, P. V. Cravo, V. E. do Rosário, C. Novo, A. Domingos. X Congresso Nacional de Biotecnologia Biotec2003, Lisboa. 11. New aspartic protease from the cattle parasite Babesia bovis. S. P. Teixeira, T. Martins, A. Leitão, V. E. do Rosário, C. Novo, A. Domingos. X Congresso Nacional de Biotecnologia Biotec2003, Lisboa. 12. Activity of the Plasmodium chabaudi recombinant haemoglobinase plasmepsin. T. M. Martins, D. Wyatt, P. V. Cravo, V. E. do Rosário, C. Novo, C. Berry, A. Domingos. X Congresso Nacional de Biotecnologia Biotec2003, Lisboa. 13. Recombinant expression of the Plasmodium chabaudi cysteine protease chabaupain 1. R. A. Caldeira, T. M. Martins, V. E. do Rosário, C. Novo, A. Domingos. X Congresso Nacional de Biotecnologia Biotec2003, Lisboa. 14. dsrna purification by antibody affinity chromatography. C. Baptista, F. Cardoso, C. Novo. X Congresso Nacional de Biotecnologia Biotec2003, Lisboa. 15. Isolation of scfv from Tomlinson I+J library antibodies against Olive virus 1 A. Custodio, F. Cardoso, C. Novo, M.R. Felix, M.I. Clara. X Congresso Nacional de Biotecnologia Biotec2003, Lisboa. 16. Expression and activity of the Plasmodium chabaudi recombinant haemoglobinase plasmepsin 1. Martins, T.M., Wyatt, D., Cravo, P.V.I., Novo, C., Berry, C., Domingos, A. 11 th European Congress on Biotechnology, P620, Basel, Switzerland, Nitrile hydratase PCR detection from soil metagenome. Lourenço, P.M.L., Almeida, T., Mendonça, D., Simões, F., Novo, C. Metagenomics, from microbial diversity to function, P 9, p.40, Darmstad, Alemanha, 2003.

4 18. Neutralizing immune response in mice immunized with envelope glycoproteins from the primary isolate HIV2ALI, J. Marcelino, H. Barroso, C. Novo, R. Antunes, P. Matoso, C. Nilsson, E. Bjorling, N. Taveira. Keystone Symposia, P430, Alberta, Canadá Theoretical models of Plasmodium chabaudi proteases. C.Novo, T.M. Martins, R.A. Caldeira, V.E. do Rosário, A. Domingos. XIII Congresso Nacional da Soc. Portuguesa de Bioquímica, S6 (P15), p 70, Lisboa, Nitrile hydratase from Rhizobium radiobacter. Elucidation of the nucleotide sequence. T. Almeida, P.M.L. Lourenço, D. Mendonça, F. Simões, C. Novo. XIII Congresso Nacional da Soc. Port. de Bioquímica, S6 (P40) p 76, Lisboa, Selection of scfv and peptides binding phages against dsrna from phage display libaries using paramagnetic particles. F. Cardoso, A. Custódio, C. Novo. XIII Congresso Nacional da Soc. Port. de Bioquímica, S9 (P3) p 102, Lisboa, Production of monoclonal antibodies towards Olive latent virus 1. A.I. Custódio, F. cardoso, C. Novo, M.R. Félix, M.I. Clara. XIII Congresso Nacional da Soc. Port. de Bioquímica, S9 (P15) p 105, Lisboa, Detection of endogen ds RNA. Comparative methods. F. Cardoso, C. Baptista, C. Novo. XIII Congresso Nacional da Soc. Port. de Bioquímica, S9 (P17) p 105, Lisboa, Production of multiple antigenic peptides for aspartic proteases from Plasmodium chabaud immunolocalization. J.I. Pombo, V.E. do Rosário, C. Novo, A. Domingos. XIII Congresso Nacional da Soc. Port. de Bioquímica, S9 (P29) p 108, Lisboa, Cysteine protease from the rodent parasite Plasmodium chabaudi: potential model for cysteine proteases of human malaria parasites. R. Caldeira, T.M. Martins, C. Novo, V.E. do Rosário, A. Domingos. XIII Congresso Nacional da Soc. Port. de Bioquímica, S10 (P2) p 112, Lisboa, Preliminary results on the presence of proteases on malaria patients urine, A. Armada, V.E. do Rosário, C. Novo, A. Domingos, 3 rd European Congress on Tropical Medecine and International Health, Acta Tropica, 83 (Suppl 1) P131, Lisbon The first cysteine protease found in Plasmodium chabaudi, a rodent malaria parasite, R. A. Caldeira, T.M. Martins, V.E. do Rosário, C. Novo, A. Domingos, 3 rd European Congress on Tropical Medecine and International Health, Acta Tropica, 83 (Suppl 1) P080, Lisbon Aspartic proteases of the rodent malaria parasite Plasmodium chabaudi, T.M. Martins, V.E. do Rosário, C. Novo, A. Domingos, 3 rd European Congress on Tropical Medecine and International Health, Acta Tropica, 83 (Suppl 1) P142, Lisbon Characterization of a 45kDa glycoprotein in urine of patients with malaria, A. Armada, C. Novo, V. Rosário, M. Hommel, 3 rd European Congress on Tropical Medecine and International Health, Acta Tropica, 83 (Suppl 1) P081, Lisbon Design, production and evaluation of new envelope derived HIV 2 antigens and immunogens, N. Taveira, H. Barroso, J. Marcelino, C. Novo, J. Moniz Pereira, 7 th European Conference on Experimental AIDS Research (ECEAR), WS64, Genoa, Italy, Effect of Agrobacterium rhizogenes infection on in vitro rooting of Vitis vinifera. Confirmation of the transgenic nature of the roots, T.M. Martins, P.M.L. Lourenço, A. Domingos, C. Novo, Micro2001, P147, Congresso Nacional de Microbiologia, Póvoa do Varzim, Rodent parasite P. chabaudi s a model for proteinases in human malaria, T.M. Martins, C. Dias, J. Ferreira, C. Novo, A. Domingos, V. E. Rosário, FEBS 27 th Meeting, Lisboa, EBJ 268, Supplement 1, PW1 027, 2001.

5 33. A monoclonal antibody against the cinnamomin from Phytophtora cinnamomi. Ferreira, S.M.S., Custódio, A.I., Duclos, J., Clemente, A., Novo, C. 11 th Congress of MPU/3 rd Congress of SPF, Évora.2001, P18.