ROUTINE SCREENING AND QUANTITATION. Cliquid Software and imethod Applications ONE TOUCH PRODUCTIVITY
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1 ROUTINE SCREENING AND QUANTITATION Cliquid Software and imethod Applications ONE TOUCH PRODUCTIVITY
2 Simple. Flexible. Powerful. From running samples to generating results, AB SCIEX Cliquid Software simplifies routine LC/MS/MS testing with an intuitive point-and-click interface and a configurable four-step workflow. A setup wizard guides the user through the process of adding tests to a preconfigured test menu that automatically acquires data and generates reports according to established standard operating procedures. A highly customizable solution Cliquid Software gives you flexibility in terms of the applications you can run and the content that individual users see. You can configure Cliquid Software to run a single application or a series of applications. Set method specifics such as internal standard concentrations or injection volumes to simplify the graphical interface and minimize user error. Set report defaults for each application to ensure that results are always represented in a consistent, approved manner. You can choose from a number of included report templates or customize your own. It s as easy as choosing a test, building (or importing) a sample list, choosing a report, and submitting your samples. Support for a variety of systems Cliquid Software is compatible with all AB SCIEX Triple Quad and QTRAP series instruments. Cliquid Software is also compatible with all industry leading HPLC and online sample preparation systems and is continually updated to support new HPLC releases. Change your HPLC configuration at anytime, with minimal changes to Cliquid Software. Perform a variety of experiments Since Cliquid Software is compatible with the AB SCIEX Triple Quad and QTRAP series instruments, you can use a number of different analysis workflows. For example, perform quantitative analysis using a single MRM transition or multiple transitions in an automated fashion, including MRM ratio experiments. Report results automatically in batch mode, after all samples have been analyzed, or individually, after each sample has been run. You can also add previously processed data to newly generated data, and use previously run standard curves with the current batch. Secure, easy-to-use, customizable four-step workflow MRM catalogue for easy method creation Workflows for quantitative, targeted, and general unknown screening with optional automated reporting Automatic library searching Bi-directional LIS/LIMS support including barcode reading Interactive review of quantitative results Access to other application-specific or efficiency gaining tools Compound-class specific imethod Applications The list of tests can be customized for any laboratory using a simple point and click workflow.
3 Create new tests Run pre-configured imethod Applications You can create new Cliquid Software tests in one of two ways: 1) Use existing acquisition and quantitation methods developed in Analyst Software, or 2) create a new test using an MRM Catalogue. In either case, you can add one or more report templates to the test and save the method for use in the Cliquid Software test menu. The MRM Catalogue The MRM Catalogue contains up to six optimized MRM transitions as well as the corresponding retention time imethod Applications for Cliquid Software are the easiest way to harness the power of LC/MS/MS for food safety testing, environmental analysis, clinical research, and forensic toxicology. Simply download a lab-proven method from the web or DVD into Cliquid Software and you are ready to implement it as an imethod Application in your lab. All the LC and MS specific instrument parameters are downloaded during installation. All the information you need to start running a test immediately is included sample preparation, LC methods, optimized MS methods, processing methods, reporting templates, and complete method documentation. information for a series of application specific analytes. Choose the MRM Catalogue that meets your need, including The value of imethod Applications imethod Application Center one for pesticides, containing 540 compounds. Note that imethod Applications not only provide instant methods to imethod Applications are available for a variety of routine assays these catalogues are available separately and do not come reduce the need for method development, they also enable you for food safety testing, water quality testing, clinical research, and as standard with Cliquid Software. to create new or customize existing methods using the MRM forensic toxicology. For a complete list and description of imethod Catalogue, reducing development costs and development time Applications available from AB SCIEX, please visit our imethod from months to days. Starting with an imethod Application can Application center at one for drugs of abuse, containing 1250 compounds, and Secure user login save you months of time and effort and thousands of dollars When the Cliquid Software is launched, a login page appears that in the deployment of a new test. requires a password. Once the password is entered, the graphical interface appears with content based upon the user rights and personalized settings created during setup. Depending on these rights, some users will only be able to run samples and generate reports, while users with more extensive access permission can create, edit, and activate tests. The MRM Catalogue allows maximum flexibility to add, delete, and edit compounds.
4 Support for a wide range of applications and efficiency improving tools LIS/LIMS compatibility Through the use of a middleware solution, Cliquid Software can support a variety of bi-directional workflows between the LC/MS/MS system and the Laboratory Information System (LIS) or Laboratory Information Management System (LIMS). Cliquid ChemoView Software MPX -2 High Throughput System Review and release results to LIS/LIMS Chain of custody workflows Cliquid ChemoView Software uses the processing power of the ChemoView Software and adds data acquisition to provide a complete automated solution. With ChemoView Software, you can quickly screen large sample sets for multiple components using flow injection analysis on AB SCIEX triple quadrupole LC/MS/MS systems. This provides a powerful workflow for researching into inborn errors of metabolism (IEM), metabolic studies, lipid and endocrine studies, acylcarnitine profiling, and monitoring compounds such as amino acids (AA), acylcarnitines (AC), sugars, fatty acids, peptides, and proteins. MPX-2 High Throughput System is an end-to-end solution to accelerate LC/MS/MS productivity for a number of applications including pharmaceutical discovery and development, clinical research, and forensic toxicology. Using MPX-2 with Cliquid Software you can double the throughput of AB SCIEX Triple Quad and QTRAP mass spectrometers by allowing concurrent LC/MS/MS analysis of two streams into a single mass spectrometer. Cliquid Software creates a single point of control for managing the complete workflow to provides the most efficient solution of its kind within the life-science industry. Full support of targeted and untargeted screening workflows Combined with QTRAP technology, Cliquid Software enables automated screening, quantitation, and identification of targeted analytes, as well as spectral library searching to support untargeted screening experiments. Data acquisition, library searching, and reporting are completely automated within the Cliquid Software, providing a simple solution for comprehensive workflows. Once data is collected from the LC/MS/MS system, the results can be reviewed and then released to the LIS/LIMS via the middleware. Using either the Cliquid Software reporting functionality or through the middleware itself, you can customize the format and content of the exported results including the ability to perform simple mathematical calculations. Importing worklists You can import worklists into the Cliquid Software from a variety of LIS/LIMS or offline sample preparation systems through the use of middleware. These include workflows that involve barcodes or simply a single downloaded worklist. You can also set up worklist templates in the middleware to facilitate the addition of standards, quality controls and samples into the batch. Cliquid Software can read barcodes from vials when using the appropriate LC system. In the simplest implementation, the barcode from each vial is logged in the data file or for more elaborate workflows; Cliquid Software compares the expected barcode from the worklist to the actual barcode. Any differences are flagged to the user. Vitamin D Analysis Retention Time: 2.67 minutes Q1/Q3: / Da Sample FCD Expected RT: 2.66 minutes Date: 1/22/2011 8:55:48 PM Analyte Name: Lorazepam Collision Energy =35± 15 ev 4 th 100 th OH-Vitamin- OH-Vitamin- Time, min Time, min %B Time (Min) Two fold increase in throughput 1.9 times increase in thoughput 100 injections made from commercially available serum calibrator and controls using 2 stream approach Singleplex Run Time: 422 minutes Multiplex Run TIme: 220 minutes Time Saved: 3 hours 22 minutes Lorazepam Library Spectrum Acquired Spectrum Compound Name Peak Area (counts) Purity (%) 1 Lorazepam 1.49e Staggered injections double throughput: Using standard chromatography, peaks of interest elute from 1.7 to 2.5 minutes. By multiplexing staggered injections, a second analysis is performed during the dead time between analyses to double the number of runs performed during a 4 minute run time. Example report of a general unknown screening experiment. Export samples from Cliquid Software tests to a LIMS or LIS. Sample list depicting examples of barcodes that did not match the expected for a series of samples.
5 You invest in our technology. We invest in your success. As the world leader in mass spectrometry, AB SCIEX solutions are backed by the industry s most extensive service and support organization. With a network of service professionals, experienced compliance specialists, and over 150 PhD application scientists worldwide, we are dedicated to supporting your technical needs and helping you get the most out of your AB SCIEX systems. AB SCIEX service professionals are recognized as the most highly qualified in the industry. They are certified on our instrument platforms through a rigorous 4-step certification program, with re-certification occurring every two years. This award-winning program helps to ensure that you receive the most efficient, highest-quality, and most up-to-date service available for AB SCIEX products and technology. Choose from flexible service plans and a variety of services for the right level of support for your laboratory s needs and budget. Our customer support network is available to provide expert assistance in the use and application of AB SCIEX products through a comprehensive range of services, including application support, technical service, and training. Whether you access our service and support team by phone, , on-site visits, or through our innovative remote monitoring technology, you can be confident that the AB SCIEX organization will be there for you. For more information, visit For Research Use Only. Not for use in diagnostic procedures AB SCIEX. The trademarks mentioned herein are the property of AB Sciex Pte. Ltd. or their respective owners. AB SCIEX is being used under license /2011 Headquarters 110 Marsh Drive Foster City CA USA Phone International Sales For our office locations please call the division headquarters or refer to our website at
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