DNA & Protein Synthesis Exam
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1 DNA & Protein Synthesis Exam DO NOT WRITE ON EXAM EXAM # VER. B Multiple choice Directions: Answer the following questions based on the following diagram. (1pt. each) 5. The above nucleotide is purine b. An enzyme c. A pyramidine d. None of the above 6. The DNA molecule is in the shape of a(n) a. Single strand b. Alpha helix c. Beta pleated sheet d. Double helix 7. The mrna Molecule is in the shape of a(n) a. Single strand b. Alpha helix c. Beta pleated sheet d. Double helix 1. In the above item A refers to the 2. In the above item B refers to the 3. In the above item C refers to the 4. The sugar molecule represented consists of Carbons. a. 3 b. 4 c. 5 d The Backbone of the DNA molecule is made of which components of the nucleotide from the above diagram? & B b. B & C c. A & C d. B only 9. The component which binds complimentarily according to base pairing rules is item. b. B c. C d. None of the above 10. According to base pairing rules with DNA "A" bonds only to b. C c. T d. U e. G 1
2 11. The "Central Dogma" of genetics states the usual order of events in protein synthesis proceeds along which of the following sequences? a. RNA DNA Protein b. DNA Ribosome RNA c. DNA Protein RNADNA RNA Protein Matching: Directions: Match the enzyme with the correct function of that enzyme 12. DNA Helicase A. Binds RNA primer to lagging strand of DNA 13. RNA polymerase B. "Unzips" DNA strand 14. Primase C. Adds deoxyribonucleotides along a DNA strand 15 DNA polymerase D. Adds ribonucleotides along an RNA strand 16. Ligase E. Links DNA fragments on lagging strand Directions: For the following questions, refer to the diagram below n 19. t Item labeled B refers to a. The codon b. Amino acid attachment site c. The anticodon d. A hydrogen bond Directions: For the following questions, refer to the diagram below 17. The above diagram refers to a molecule of a. mrna b. DNA c. trna d. rrna 18. Item labeled A refers to a. The codon b. Amino acid attachment site c. The anticodon d. A hydrogen bond 20. Given the above DNA strand. If replication was proceeding from bottom to top, which strand would be the "lagging" Strand? b. B c. None of the above 2
3 Directions: For the following questions, refer to the diagram below and match the letter of the indicated structure. 30. The DNA strand from which mrna is transcribed is known as the a. Leading Strand b. Sense strand c. Lagging Strand d. Nonsense strand e. None of the above 31. Reproductive cloning would most likely result in a. Republican domination of the world b. Transplantable hearts c. Nervous tissue generation d. A human clone baby 21. Large ribosomal subunit 22. Ribosome 23. mrna strand 24. Polypeptide 25. Small subunit 26. The ribosomes are made in which cellular structure in the eukaryotic cell. a. Rough endoplasmic reticulum b. Ribosobium complex c. Nucleolus d. Smooth endoplasmic reticulum 27. The experiment in which DNA and proteins were isolated, and used to transform rough strain bacteria was cunducted by which researcher(s)? a. Watson & Crick b. Fredrick Griffith c. Avery, McCarthy & McLeod d. Hershey & Chase 32. RNA molecules differ from DNA in the following ways except a. I its smaller b. It is single stranded c. It contains no adenine d. It has ribose, not deoxyribose as it's 5 carbon sugar e. None of the above 33. The three basepair sequence found on an mrna strand is called the a. Codon b. Anticodon c. Coda d. Genome 34. DNA replication occurs in which direction? a. 5' 3' b. 3' 5' c. Both a & b d. Neither a or b 28. DNA gel electrophoresis separates DNA fragments by a. Density b. Strength of charge c. Number of adenine subunits d. Size 29. A mutation which results in the changing of all the codons following the error is called a mutation. a. a. frameshift b. a point only mutation c. Chromosomal mutation d. Germ mutation 3
4 4
5 NAME: DATE: PER: VER:.A Directions: Using the genetic code table below, take the following DNA sequencee and complete the following information below. (3 points each step) DNA Sequence: TACCCC CATTTAA ACATACC CACT 35. Complimentary DNA strand: 36. mrna Strand: 37. Polypeptide sequence: Directions: Use the restriction map below to complete the following electrophoresis problem. Draw the products of the digestion of plasmid Stan2 (left), into the electrophoresiss gel diagram (right) 38. Plot Lane A with the digest if only EcoR1 was used 39. Plot Lane B with the digest from EcoR1, & Hind III 40. Plot Lane C with the digest from EcoR1, Hind III, & Sma1 41. Plot Lane D with the digest from all the restriction enzymes. 5
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