Microscopes. Eukaryotes Eukaryotic cells are characterized by having: DNA in a nucleus that is bounded by a membranous nuclear envelope

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1 CH 6 The Cell Microscopy Scientists use microscopes to visualize cells too small to see with the naked eye. In a light microscope (LM), visible light is passed through a specimen and then through glass lenses. Lenses refract (bend) the light, so that the image is magnified. Microscopes LMs can magnify effectively to about 1,000 times the size of the actual specimen. Various techniques enhance contrast and enable cell components to be stained or labeled. Most subcellular structures, including organelles (membrane-enclosed compartments), are too small to be resolved by an LM. Two basic types of electron microscopes (EMs) are used to study subcellular structures. Scanning electron microscopes (SEMs) focus a beam of electrons onto the surface of a specimen, providing images that look 3-D. Transmission electron microscopes (TEMs) focus a beam of electrons through a specimen. TEMs are used mainly to study the internal structure of cells. Eukaryotic cell compartmentalization of functions The basic structural and functional unit of every organism is one of two types of cells: prokaryotic or eukaryotic. Only organisms of the domains Bacteria and Archaea consist of prokaryotic cells. Protists, fungi, animals, and plants all consist of eukaryotic cells. Prokaryotic vs. Eukaryotic Basic features of all cells Plasma membrane Semifluid substance called cytosol Chromosomes (carry genes) Ribosomes (make proteins) Prokaryotes Prokaryotic cells are characterized by having No nucleus DNA in an unbound region called the nucleoid No membrane-bound organelles Cytoplasm bound by the plasma membrane Eukaryotes Eukaryotic cells are characterized by having: DNA in a nucleus that is bounded by a membranous nuclear envelope

2 Membrane-bound organelles Cytoplasm in the region between the plasma membrane and nucleus Eukaryotic cells are generally much larger than prokaryotic cells. The plasma membrane is a selective barrier that allows sufficient passage of oxygen, nutrients, and waste to service the volume of every cell. The general structure of a biological membrane is a double layer of phospholipids. Metabolic requirements set upper limits on the size of cells. The surface area to volume ratio of a cell is critical. As the surface area increases by a factor of n2, the volume increases by a factor of n3. Small cells have a greater surface area relative to volume. Tour of an animal cell Tour of a plant cell Eukaryotic nucleus & ribosomes The nucleus contains most of the DNA in a eukaryotic cell. Ribosomes use the information from the DNA to make proteins. Nucleus The nucleus contains most of the cell s genes and is usually the most conspicuous organelle. The nuclear envelope encloses the nucleus, separating it from the cytoplasm. The nuclear membrane is a double membrane; each membrane consists of a lipid bilayer. In the nucleus, DNA is organized into discrete units called chromosomes. Each chromosome is composed of a single DNA molecule associated with proteins. The DNA and proteins of chromosomes are together called chromatin. Chromatin condenses to form discrete chromosomes as a cell prepares to divide. The nucleolus is located within the nucleus and is the site of ribosomal RNA (rrna) synthesis. Ribosomes Ribosomes are particles made of ribosomal RNA and protein. Ribosomes carry out protein synthesis in two locations: In the cytosol (free ribosomes) On the outside of the endoplasmic reticulum or the nuclear envelope (bound ribosomes)

3 Endomembrane System Components of the endomembrane system: Nuclear envelope Endoplasmic reticulum Golgi apparatus Lysosomes Vacuoles Plasma membrane These components are either continuous or connected via transfer by vesicles. ER: the factory The endoplasmic reticulum (ER) accounts for more than half of the total membrane in many eukaryotic cells. The ER membrane is continuous with the nuclear envelope. There are two distinct regions of ER: Smooth ER, which lacks ribosomes Rough ER, surface is studded with ribosomes Smooth ER The smooth ER: Synthesizes lipids Metabolizes carbohydrates Detoxifies drugs and poisons Stores calcium ions Rough ER The rough ER: Has bound ribosomes, which secrete glycoproteins (proteins covalently bonded to carbohydrates). Distributes transport vesicles, proteins surrounded by membranes. Is a membrane factory for the cell. Golgi Apparatus: The Golgi apparatus consists of flattened membranous sacs called cisternae. Functions of the Golgi apparatus: Modifies products of the ER Manufactures certain macromolecules Sorts and packages materials into transport vesicles Lysosomes A lysosome is a membranous sac of hydrolytic enzymes that can digest macromolecules. Lysosomal enzymes can hydrolyze proteins, fats, polysaccharides, and nucleic acids. Lysosomal enzymes work best in the acidic environment inside the lysosome. Some types of cell can engulf another cell by phagocytosis; this forms a food vacuole A lysosome fuses with the food vacuole and digests the molecules

4 Lysosomes also use enzymes to recycle the cell s own organelles and macromolecules, a process called autophagy Vacuoles A plant cell or fungal cell may have one or several vacuoles, derived from endoplasmic reticulum and Golgi apparatus. Food vacuoles are formed by phagocytosis. Contractile vacuoles, found in many freshwater protists, pump excess water out of cells. Central vacuoles, found in many mature plant cells, hold organic compounds and water. Review Mitochondria Mitochondria are the sites of cellular respiration, a metabolic process that uses oxygen to generate ATP. Chloroplasts, found in plants and algae, are the sites of photosynthesis. Peroxisomes are oxidative organelles. Origins related to bacteria Mitochondria and chloroplasts have similarities with bacteria: Enveloped by a double membrane Contain free ribosomes and circular DNA molecules Grow and reproduce somewhat independently in cells Endosymbiont Theory An early ancestor of eukaryotic cells engulfed a nonphotosynthetic prokaryotic cell, which formed an endosymbiont relationship with its host. The host cell and endosymbiont merged into a single organism, a eukaryotic cell with a mitochondrion. At least one of these cells may have taken up a photosynthetic prokaryote, becoming the ancestor of cells that contain chloroplasts. Mitochondria Mitochondria are in nearly all eukaryotic cells. They have a smooth outer membrane and an inner membrane folded into cristae. The inner membrane creates two compartments: intermembrane space and mitochondrial matrix. Some metabolic steps of cellular respiration are catalyzed in the mitochondrial matrix. Cristae present a large surface area for enzymes that synthesize ATP. Chloroplasts: Capture of Light Chloroplasts contain the green pigment chlorophyll, as well as enzymes and other molecules that function in photosynthesis.

5 Chloroplasts are found in leaves and other green organs of plants and in algae. Chloroplast structure includes: Thylakoids, membranous sacs, stacked to form a granum Stroma, the internal fluid The chloroplast is one of a group of plant organelles, called plastids. Peroxisomes Peroxisomes are specialized metabolic compartments bounded by a single membrane. Peroxisomes produce hydrogen peroxide and convert it to water. Peroxisomes perform reactions with many different functions. How peroxisomes are related to other organelles is still unknown. Cytoskeleton The cytoskeleton is a network of fibers extending throughout the cytoplasm. It organizes the cell s structures and activities, anchoring many organelles. It is composed of three types of molecular structures: Microtubules Microfilaments Intermediate filaments Support and Motility The cytoskeleton helps to support the cell and maintain its shape. It interacts with motor proteins to produce motility. Inside the cell, vesicles can travel along monorails provided by the cytoskeleton. Recent evidence suggests that the cytoskeleton may help regulate biochemical activities. Components of the Cytoskeleton Three main types of fibers make up the cytoskeleton: Microtubules are the thickest of the three components of the cytoskeleton Microfilaments, also called actin filaments, are the thinnest components Intermediate filaments are fibers with diameters in a middle range Microtubules Microtubules are hollow rods about 25 nm in diameter and about 200 nm to 25 microns long. Functions of microtubules: Shaping the cell Guiding movement of organelles Separating chromosomes during cell division In many cells, microtubules grow out from a centrosome near the nucleus. The centrosome is a microtubule-organizing center. In animal cells, the centrosome has a pair of centrioles, each with nine triplets of microtubules arranged in a ring.

6 Cilia & Flagella Microtubules control the beating of cilia and flagella, locomotor appendages of some cells. Cilia and flagella differ in their beating patterns. Paramecium Cilia Cilia and flagella share a common structure A core of microtubules sheathed by the plasma membrane A basal body that anchors the cilium or flagellum A motor protein called dynein, which drives the bending movements of a cilium or flagellum Microfilaments Microfilaments are solid rods about 7 nm in diameter, built as a twisted double chain of actin subunits. The structural role of microfilaments is to bear tension, resisting pulling forces within the cell. They form a 3-D network called the cortex just inside the plasma membrane to help support the cell s shape. Bundles of microfilaments make up the core of microvilli of intestinal cells. Microfilaments that function in cellular motility contain the protein myosin in addition to actin. In muscle cells, thousands of actin filaments are arranged parallel to one another. Thicker filaments composed of myosin interdigitate with the thinner actin fibers. Intermediate Filaments Intermediate filaments range in diameter from 8 12 nanometers, larger than microfilaments but smaller than microtubules. They support cell shape and fix organelles in place. Intermediate filaments are more permanent cytoskeleton fixtures than the other two classes. Extracellular Structures The cell wall is an extracellular structure that distinguishes plant cells from animal cells. Prokaryotes, fungi, and some protists also have cell walls. The cell wall protects the plant cell, maintains its shape, and prevents excessive uptake of water. Plant cell walls are made of cellulose fibers embedded in other polysaccharides and protein. Functions Support Adhesion Movement Regulation Extracellular Structures

7 Animal cells lack cell walls but are covered by an elaborate extracellular matrix (ECM). The ECM is made up of glycoproteins such as collagen, proteoglycans, and fibronectin. ECM proteins bind to receptor proteins in the plasma membrane called integrins. Extracellular Structures Neighboring cells in tissues, organs, or organ systems often adhere, interact, and communicate through direct physical contact. Intercellular junctions facilitate this contact. There are several types of intercellular junctions Plasmodesmata Tight junctions Desmosomes Gap junctions Plant Cells Plasmodesmata are channels that perforate plant cell walls. Through plasmodesmata, water and small solutes (and sometimes proteins and RNA) can pass from cell to cell. Animal Cells At tight junctions, membranes of neighboring cells are pressed together, preventing leakage of extracellular fluid. Desmosomes (anchoring junctions) fasten cells together into strong sheets. Gap junctions (communicating junctions) provide cytoplasmic channels between adjacent cells.