IDEXX SNAP. Reader Analyzer. Operator s Manual

Transcription

1 IDEXX SNAP Reader Analyzer Operator s Manual

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3 Proprietary Rights Notice Information in this document is subject to change without notice. Companies, names and data used in examples are fictitious unless otherwise noted. No part of this document may be reproduced or transmitted in any form or by any means, electronic, mechanical or otherwise, for any purpose, without the express written permission of IDEXX Laboratories. IDEXX may have patents or pending patent applications, trademarks, copyrights or other intellectual or industrial property rights covering this document or subject matter in this document. The furnishing of this document does not give a license to these property rights except as expressly provided in any written license agreement from IDEXX Laboratories IDEXX Laboratories, Inc. All rights reserved All /TM marks are owned by IDEXX Laboratories, Inc. or its affiliates in the United States and/or other countries. One IDEXX Drive Westbrook, Maine USA idexx.com iii

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5 Contents Preface... vii About This Manual... vii International Symbol Descriptions... vii IDEXX Customer Support Information... ix 1 Getting Started Introduction SNAP Test Kits for Use with the SNAP Reader Analyzer SNAP Reader Specifications Handling the SNAP Reader Safety Precautions Important Electrical Safety Precautions Installing the SNAP Reader Analyzer Components Installation Running Quantitative SNAP Tests Using the SNAP Reader Analyzer Running a SNAP Cortisol Test Running a SNAP Bile Acids Test Running a SNAP T 4 (Thyroxine) Test Calibration and Maintenance Procedures SNAP Reader Calibration Procedure General Maintenance Procedures Powering Off Case Cleaning Incubator Cleaning SNAP Entry Port Cleaning Messages and Error Codes VetTest Analyzer Displayed or Printed Messages SNAP Reader Analyzer Error Codes and LCD Display Messages Troubleshooting (when no messages are displayed/printed)...21

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7 Preface About This Manual Use This Chapter... To Learn About... 1 Getting Started How the analyzer works Analyzer specifications Safety precautions 2 Installing the SNAP Reader Analyzer Analyzer components Installing the analyzer with other IDEXX analyzers 3 Running SNAP Tests How to run SNAP Cortisol, Bile Acids and T 4 Tests 4 Calibration and Maintenance Procedures How to calibrate the SNAP Reader analyzer General maintenance and cleaning procedures 5 Messages and Error Codes How to respond to messages and error codes International Symbol Descriptions International symbols are often used on packaging to provide a pictorial representation of particular information related to the product (such as expiration date, temperature limitations, batch code, etc.). IDEXX Laboratories has adopted the use of international symbols on our analyzers, product boxes, labels, inserts and manuals in an effort to provide our users with easy-to-read information. If you are unsure of the meaning for a particular symbol, see the table below for a description of each symbol that can be found on IDEXX Laboratories packaging. Symbol Description Symbol Description Use by A utiliser avant Verwendbar bis Usare entro Usar antes de 使 用 期 限 Batch Code (Lot) Code de lot (Lot) Chargenbezeichnung (Partie) Codice del lotto (partita) Código de lote (Lote) ロット 番 号 Temperature limitation Température limite Zulässiger Temperaturbereich Temperatura limite Limitación de temperatura 保 存 温 度 ( 下 限 ) Upper limit of temperature Limite supérieure de température Temperaturobergrenze Limite superiore di temperatura Limite superior de temperatura 保 存 温 度 ( 上 限 ) vii

8 IDEXX SNAP Reader Analyzer Operator s Manual Symbol Description Symbol Description Serial Number Numéro de série Seriennummer Numero di serie Número de serie シリアル 番 号 Catalog Number Numéro catalogue Bestellnummer Numero di catalogo Número de catálogo 製 品 番 号 Authorized Representative in the European Community Représentant agréé pour la C.E.E. Autorisierte EG-Vertretung Rappresentante autorizzato nella Comunitá Europea Representante autorizado en la Comunidad Europea EC 内 の 正 規 販 売 代 理 店 Manufacturer Fabricant Hersteller Ditta produttrice Fabricante 製 造 元 Caution, consult accompanying documents Attention, consulter les documents joints Achtung, Begleitdokumente beachten Attenzione, consultare la documentazione allegata Precaución, consultar la documentación adjunta 注 意 添 付 文 書 をご 参 照 ください 2 Consult instructions for use Consulter la notice d utilisation Gebrauchsanweisung beachten Consultare le istruzioni per l uso Consultar las instrucciones de uso 取 扱 説 明 書 をご 参 照 ください Keep away from sunlight Conserver à l abri de la lumière Vor direkter Sonneneinstrahlung schützen Mantener alejado de la luz solar Tenere lontano dalla luce diretta del sole 遮 光 してください WEEE Directive 2002/96/EC Directive 2002/96/CE (DEEE) WEEE-Richtlinie 2002/96/EG Directiva 2002/96/CE RAEE Direttiva RAEE 2002/96/CE 廃 電 気 電 子 機 器 指 令 (WEEE Directive 2002/96/EC) Biological Risks Risques biologiques Biogefährlich Rischi biologici Riesgos biológicos 生 物 学 的 リスク Do Not Reuse Usage unique Nicht wiederverwenden No reutilizarw Non riutilizzare 再 利 用 しないでください viii

9 Preface IDEXX Customer Support Information IDEXX Representative: Telephone/Voice Mail: United States IDEXX Laboratories Inc. One IDEXX Drive Westbrook, Maine USA Toll-Free Technical Support Main Telephone Number Toll-Free Fax idexx.com The Netherlands IDEXX Laboratories B.V. P.O. Box EK Hoofddorp The Netherlands Toll-Free Technical Support Telephone Toll-Free Fax Fax France IDEXX S.A.R.L. Bâtiment Floride - Eragny Parc 11 Allée Rosa, Luxembourg Eragny, sur Oise France Toll-Free Technical Support Telephone Fax Germany IDEXX GmbH Mörikestraße 28/3 D Ludwigsburg Germany Toll-Free Technical Support Telephone Fax Italy IDEXX Laboratories Italia, S.r.l. Via Canova Milano Italy Toll-Free Technical Support Telephone Fax Spain IDEXX Laboratorios, S.L. c/ Plom n 2-8, Barcelona Spain Toll-Free Technical Support Telephone Fax United Kingdom IDEXX Laboratories Ltd. Milton Court, Churchfield Road Chalfont St Peter Buckinghamshire SL9 9EW United Kingdom Toll-Free Technical Support Telephone Fax Australia IDEXX Laboratories Pty. Ltd. Metro Centre Unit 20, South Street Rydalmere, New South Wales 2116 Australia Toll-Free Technical Support Telephone Fax Canada IDEXX Laboratories Canada Corporation C/O Apple Express 5300 Satellite Drive Mississauga, Ontario L4W 5J2 Canada Toll-Free Technical Support Telephone Toll-Free Fax Fax idexx.com Japan IDEXX Laboratories KK Kitano, Mitaka-shi, Tokyo Japan Toll-Free Technical Support Telephone Fax ix

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11 1 Getting Started 1.1 Introduction The IDEXX SNAP Reader Analyzer is a digital camera and image-analysis instrument. It stores and processes images of SNAP tests according to the protocol of the specific, individual SNAP test designed for use with the analyzer. The SNAP Reader analyzer then uses custom software to evaluate the results of the test being run and reports the results. The SNAP Reader, working with the IDEXX VetTest Chemistry Analyzer, walks you through the test protocol via online instructions and audible signals. A sample incubator and an automatic timing function add convenience when running the SNAP tests. The analyzer takes digital pictures as test results are developing and its software uses algorithms specific to the test to calculate the test results from these digital images. The test results display on the SNAP Reader LCD screen and on the VetTest analyzer s screen. You can print these results using the VetTest analyzer or an external printer. Standard curve information for the different manufacturing lots of SNAP tests are encoded on the tests and found on the kit packaging. The SNAP Reader must be on for 30 minutes to reach the correct operating temperature. The SNAP calibration device must be run daily prior to running an assay. Individual SNAP tests must be at room temperature prior to beginning the testing protocol. An individual SNAP test takes 30 minutes to acclimate to room temperature. The IDEXX SNAP Reader Analyzer is for veterinary use only. 1.2 SNAP Test Kits for Use with the SNAP Reader Analyzer Only certain SNAP test kits are compatible with the SNAP Reader analyzer. These tests are clearly marked on the kit label and in the product insert as For use only with the SNAP Reader. To order SNAP Reader-compatible SNAP test kits, contact your IDEXX distributor. 1.3 SNAP Reader Specifications Species Units Sample Size Result Timing Canine Feline Equine T 4 Cortisol Bile Acids U.S., French or S.I. units The SNAP Reader requires: μl of sample to run a cortisol test μl of sample to run a T 4 test 100 μl of sample to run a bile acids test The SNAP Reader takes as little as six minutes to run a T 4, cortisol or bile acids assay and report the results. 1

12 IDEXX SNAP Reader Analyzer Operator s Manual Assay Kits Calibration Technology Power Supply Available assays are T 4, cortisol and bile acids. Assay devices are packaged in boxes of six or fifteen T 4 devices, and six bile acids or cortisol devices. The SNAP Reader must be calibrated daily using the CalSNAP device that is supplied with the reader. This process takes less than two minutes to complete. Assays are calibrated at IDEXX and have lot-specific calibration information encoded on each device. Colorimetric competitive enzyme-linked immunosorbent assays (ELISAs) are read on an optical analyzer with digital processing. Universal power supply Operating Temperature C (66 81 F) and relative humidity between 30% and 85% Software Accessories Selectable Dutch, English, French, German, Italian and Spanish interfaces Calibration device (CalSNAP ), 300-μL manual pipettor, individual SNAP devices for bile acids, T 4 and cortisol 1.4 Handling the SNAP Reader Use extreme care when handling the SNAP Reader analyzer. Always keep the analyzer upright. The computer circuitry and optical sensors can be damaged if it is dropped or jarred, particularly when it is on. If you must move the analyzer any distance, use the original packing materials. 1.5 Safety Precautions As with any precision instrument, safety precautions must be taken to ensure that the SNAP Reader analyzer functions properly. Be sure to take the following safety precautions: Do not stack other equipment or containers on top of the analyzer. Keep the analyzer away from sources of heat or flames, e.g., Bunsen burners. Do not place or operate the analyzer near X-ray equipment, photocopiers or other devices that generate static or magnetic fields, e.g., transformers. Protect your equipment from damp conditions or wet weather. Keep the analyzer out of direct sunlight. Take care to place the analyzer in an area not exposed to bright light or a light source that points into the SNAP entry port. The SNAP Reader should be turned off nightly. Turn off the unit using the on/off switch located on the back of the analyzer. Always turn the SNAP Reader off before covering it with the dust cover. 2

13 Chapter 1: Getting Started Disconnect the power supply before cleaning. Refer to the cleaning instructions in Chapter 4: Calibration and Maintenance Procedures for correct cleaning methods. Because this instrument has a digital camera, take care to keep dust and debris away from it, especially the SNAP entry port. Do not use any of the following liquids, abrasives or aerosol sprays on or near the analyzer, as they may damage the outer case and may influence the analysis results: Organic solvents Insecticides Ammonia-based cleaners Disinfectant Ink markers Polish Sprays containing volatile liquids Room freshener Do not use aerosols, propellant solutions or solvents near the SNAP entry port; they may cloud or obscure the lens of the camera. Take care not to spill water or other fluids on the analyzer. Do not insert anything into the SNAP entry port except tests approved for use with the analyzer. The SNAP Reader contains no user-serviceable components and should only be opened by a qualified IDEXX service representative. Call IDEXX Technical Support for any service issues. 1.6 Important Electrical Safety Precautions Be sure to take the following electrical safety precautions: To avoid electrical shock, ensure the ground conductor of the power cable is connected to ground. Use only the power cable supplied with the analyzer. IDEXX recommends that the power pack be connected to a quality, dedicated AC source through a surge-protected power strip. Disconnect the power pack: If the power cord or plug becomes frayed or otherwise damaged. If anything is spilled onto the analyzer. If your analyzer is exposed to rain or any excessive moisture. If your analyzer is dropped or the case has been damaged. If you suspect that your analyzer needs service or repair. Whenever you clean the analyzer. 3

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15 2 Installing the SNAP Reader Analyzer 2.1 Components SNAP Reader SNAP Reader connector pack (VetTest D-connector, gray cable) Power pack Power cord Calibration SNAP test (CalSNAP ) 300 µl manual pipettor NOTE: You may have been shipped additional cables and/or connectors. Be sure to store them in a safe place for possible future use. 5

16 IDEXX SNAP Reader Analyzer Operator s Manual 2.2 Installation 1. Turn off your VetTest analyzer, remove the pipettor from its holder and set it on the counter. 2. Remove the VetTest rotor cover and store in a safe place for possible future use. 3. Replace the rotor cover with the SNAP Reader. 4. Plug the VetTest D-connector into the RS232 port on the back of the VetTest analyzer. 5. Plug one end of the gray cable into the VetTest D-connector and the other end into the VT port on the back of the SNAP Reader. 6

17 Chapter 2: Installing the IDEXX SNAP Reader Analyzer Warning: The following steps must be performed in exact order. 6a. Plug the power pack firmly into the back of the SNAP Reader. Plug the VetTest power cord into a surge protector that is plugged into a standard electrical outlet. 6b. Plug the SNAP Reader power cord into the SNAP Reader power pack. 6c. Plug the power pack s power cord into the surge protector. 6d. Turn on the SNAP Reader by pressing the on/off switch located on the back to the on (I) position. Let the SNAP Reader warm up for 30 minutes before use. 7. Turn on your VetTest analyzer and allow it to complete its full system check and warm-up. The SNAP icon appears on the VetTest main menu. You re now ready to use your SNAP Reader. 7

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19 3 Running Quantitative SNAP Tests 3.1 Using the SNAP Reader Analyzer 1. Select NEW SAMPLE on the VetTest main menu and follow the screen prompts to enter patient information. Select "YES" when prompted to run a SNAP test. 2. Place the tip securely on the pipettor. 3. Keep the pipettor vertical. BEFORE pressing the button, place the tip in the center of the sample. Press and release the pipettor button. The pipettor will automatically draw enough sample to perform a SNAP test. 2 Beeps 4. At 2 beeps, remove the pipettor from the sample. 5. Lower the pipette tip into an empty sample tube. Press and release the button on top of the pipettor. Keep the pipette tip in the sample tube until the analyzer beeps 3 times. 3 Beeps 9

20 IDEXX SNAP Reader Analyzer Operator s Manual 6. Fit a new disposable pipette tip over the 300-µL manual pipettor. 7. Depress the button on top of the pipettor. Lower it into the conjugate solution. Slowly release the button to draw up 300 µl of conjugate. 8. Lower the pipette containing 300 µl of conjugate into the sample tube. Depress the button on top of the pipettor to dispense the conjugate. 9. Close the sample tube and gently invert it 3 to 4 times. Place it in the SNAP Reader incubator. Press 1 to begin the VetTest five-minute timer. Note: The SNAP Bile Acids Test does not require sample incubation. 10. At the completion of the five-minute incubation, remove the sample tube and pour the entire contents into the SNAP sample well. 11. When the sample first appears in the activation circle, push firmly on the activator button until it is flush with the device body. 10

21 Chapter 3: Running SNAP Tests 12. Immediately insert the SNAP device into the SNAP Reader. The rest of the testing process occurs automatically. 3.2 Running a SNAP Cortisol Test Preparing the VetTest Analyzer 1. Enter the patient species and patient identification. When the screen reads SNAP Test Selection, press 2 on the VetTest analyzer to perform a cortisol analysis. The VetTest screen prompts you to select a cortisol protocol: Select Baseline Cortisol to obtain cortisol values for pre-dexamethasone or pre-acth administration. Dynamic range = µg/dl ( nmol/l). Select Post-Dexamethasone Cortisol to obtain values for post low- or high-dose dexamethasone administration. Dynamic range = µg/dl ( nmol/l). Select Post-ACTH Stim Cortisol to obtain values for post-acth administration. 2a. If you selected Post-Dexamethasone Cortisol, the VetTest screen prompts you to select an option: Select 4-Hour Post Low-Dose Dex when testing the first sample drawn following low-dose dexamethasone administration. Select 8-Hour Post Low-Dose Dex when testing the second sample drawn following low-dose dexamethasone administration. Select 4-Hour Post High-Dose Dex when testing the first sample drawn following high-dose dexamethasone administration. Select 8-Hour Post High-Dose Dex when testing the second sample drawn following high-dose dexamethasone administration. 2b. If you selected Post-ACTH Stim Cortisol, the VetTest screen prompts you to select an option: Select Cushing s Suspected when testing dogs suspected of having Cushing s syndrome. Dynamic range = µg/dl ( nmol/l). Select Addison s Suspected when testing dogs suspected of having Addison s disease. Dynamic range = µg/dl (14 76 nmol/l). Select Therapeutic Monitoring when testing dogs on mitotane or similar therapy. Dynamic range = µg/dl (14 76 nmol/l). Select Therapeutic Monitoring >10 µg/dl (276 nmol/l) only when the Therapeutic Monitoring option gives a >10 µg/dl (>276 nmol/l) result and a quantitative result is required. Dynamic range = µg/dl ( nmol/l). 3. Wait until the SNAP sample pipetting screen appears. Remove the VetTest pipettor from its holder and fit a new, disposable pipette tip onto it. 11

22 IDEXX SNAP Reader Analyzer Operator s Manual Pipetting the Serum Sample 4. Place the pipette tip into the serum sample; press and release the button on top of the pipettor. Keep the pipette tip in the serum sample until the analyzer beeps twice. 5. Lift the pipette tip out of the sample and lower it into an empty sample tube. 6. Press and release the button on top of the pipettor. Keep the pipette tip in the sample tube until the analyzer has beeped three times. Remove the pipettor from the sample tube. 7. Press E on the VetTest analyzer to continue. Note: Hold the pipettor upright at all times. Adding the Conjugate Solution 8. Fit a new, disposable pipette tip over the end of the 300-µL manual pipettor. Push it on firmly. 9. Fully depress and hold the button on top of the pipettor and lower the pipette tip into the conjugate solution. 10. While holding the pipette tip in the conjugate solution, slowly release the button on top of the pipettor. 11. Remove the pipette tip from the conjugate solution. Note: If air bubbles are present in the conjugate, discard the solution and repipette. 12. Lower the pipette tip containing 300 µl of conjugate into the sample tube containing the serum sample. 13. Fully depress and hold the button on top of the pipettor to dispense all of the conjugate solution. Incubating the Sample 14. Close the sample tube, gently invert it four to five times and place it in the SNAP Reader incubator. 15. Press 1 to start the VetTest s five-minute automatic timer or press 2 to time the five-minute incubation manually. IMPORTANT If you are performing only a cortisol test on the sample, press E at the Insert Slide screen to return to the VetTest main menu while the sample is incubating. If you are performing an additional analysis on this sample with other IDEXX VetLab analyzers, you can proceed with that testing while the cortisol sample is incubating. Adding the Sample to the SNAP Device 16. After the five-minute incubation, remove the sample tube from the incubator. 17. Place the SNAP cortisol device on a flat surface and pour the entire contents of the sample tube into the sample well. 18. When the sample FIRST appears in the activation circle, push down firmly on the activator button until it is flush with the device body. 12

23 Chapter 3: Running SNAP Tests Reading the SNAP Device 19. Immediately insert the SNAP cortisol device into the SNAP Reader with the sample well end inserted first. Keep the device horizontal at all times. Note: Do not remove the device from the SNAP Reader during analysis. 20. Remove the SNAP cortisol device from the SNAP Reader when prompted and press any key to continue. Cortisol results will print automatically. Note: If other tests were previously run on any IDEXX VetLab analyzer, results can be combined by following the prompts on the VetTest screen. Standard Curve Information Cortisol standard curve information is laser-etched onto each SNAP cortisol device and is read automatically. If necessary, standard curve information can also be entered manually by following instructions on the VetTest screen. The standard curve number and lot number can be found on the SNAP cortisol kit label. Important! Insert the sample well end of the SNAP device into the SNAP Reader. sample well reference reagent test reagent activation circle activator 3.3 Running a SNAP Bile Acids Test Preparing the VetTest Analyzer 1. Enter the patient species and patient identification. When the screen reads SNAP Test Selection, select Bile Acids on the VetTest analyzer. 2. The VetTest screen displays a message asking you to select a bile acids protocol. Enter 1 for Preprandial Bile Acids or 2 for Postprandial Bile Acids. 3. Wait until the SNAP sample pipetting screen appears. Remove the VetTest pipettor from its holder and fit a new, disposable pipette tip onto it. Pipetting the Serum Sample 4. Place the pipette tip into the serum sample; press and release the button on top of the pipettor. Keep the pipette tip in the serum sample until the analyzer beeps twice. 5. Lift the pipette tip out of the sample and lower it into an empty sample tube. 6. Press and release the button on top of the pipettor. Keep the pipette tip in the sample tube until the analyzer has beeped three times. Remove the pipettor from the sample tube. 7. Press E on the VetTest analyzer to continue. Note: Hold the pipettor upright at Adding the Conjugate Solution all times. 8. Fit a new, disposable pipette tip over the end of the 300-µL manual pipettor. Push it on firmly. 9. Fully depress and hold the button on top of the pipettor and lower the pipette tip into the conjugate solution. 10. While holding the pipette tip in the conjugate solution, slowly release the button on top of the pipettor. 11. Remove the pipette tip from the conjugate solution. Note: If air bubbles are present in the conjugate, discard the solution and repipette. 13

24 IDEXX SNAP Reader Analyzer Operator s Manual 12. Lower the pipette tip containing 300 µl of conjugate into the sample tube containing the serum sample. 13. Fully depress and hold the button on top of the pipettor to dispense all of the conjugate solution. 14. Close the sample tube and gently invert it four to five times. 15. Press E on the VetTest analyzer to continue. Adding the Sample to the SNAP Device 16. Place the SNAP bile acids device on a flat surface and pour the entire contents of the sample tube into the sample well. 17. When the sample first appears in the activation circle, push down firmly on the activator until it is flush with the device body. Reading the SNAP Device 18. Immediately insert the SNAP bile acids device into the SNAP Reader with the sample well end inserted first. Keep the device horizontal at all times. Note: Do not remove the device from the SNAP Reader during analysis. 19. Press any key to continue. IMPORTANT If you are performing only a bile acids test on the sample, press E at the Insert Slide screen to return to the VetTest main menu. If you are performing an additional analysis on this sample on other IDEXX VetLab analyzers, you can proceed with that testing while the bile acids analysis is in process. If necessary, refer to your VetTest Operator s Manual. 20. When prompted, remove the SNAP bile acids device from the SNAP Reader and press any key to continue. The bile acids results will print automatically. Note: If other tests were previously run on any IDEXX VetLab analyzer, results can be combined by following the prompts on the VetTest screen. Standard Curve Information Bile acids standard curve information is laser-etched onto each SNAP bile acids device and is read automatically. If necessary, standard curve information can also be entered manually by following instructions on the VetTest screen. The standard curve number and lot number can be found on the SNAP bile acids test kit label. 3.4 Running a SNAP T 4 (Thyroxine) Test Important! Insert the sample well end of the SNAP device into the SNAP Reader. sample well reference reagent test reagent activation circle activator Preparing the VetTest Analyzer 1. Enter the patient species and patient identification. When the screen reads SNAP Test Selection, press 1 on the VetTest Analyzer to perform a T 4 analysis. 2. Enter 1 for Low Dynamic Range ( µg/dl, nmol/l) or 2 for High Dynamic Range ( µg/dl, nmol/l). 3. Wait until the SNAP sample pipetting screen appears. Remove the VetTest pipettor from its holder and fit a new, disposable pipette tip onto it. 14

25 Chapter 3: Running SNAP Tests Pipetting the Sample* 4. Place the pipette tip into the sample;* press and release the button on top of the pipettor. Keep the pipette tip in the sample until the analyzer beeps twice. 5. Lift the pipette tip out of the sample and lower it into an empty sample tube. 6. Press and release the button on top of the pipettor. Keep the pipette tip in the sample tube until the analyzer has beeped three times. Remove the pipettor from the sample tube. 7. Press E on the VetTest analyzer to continue. *Supported samples are serum or lithium-heparin-based plasma. Note: Hold the pipettor upright at all times. Adding the Conjugate Solution 8. Fit a new, disposable pipette tip over the end of the 300-µL manual pipettor. Push it on firmly. 9. Fully depress and hold the button on top of the pipettor and lower the pipette tip into the conjugate solution. 10. While holding the pipette tip in the conjugate solution, slowly release the button on top of the pipettor. 11. Remove the pipette tip from the conjugate solution. Note: If air bubbles are present in the conjugate, discard the solution and repipette. 12. Lower the pipette tip containing 300 µl of conjugate into the sample tube containing the sample. 13. Fully depress and hold the button on top of the pipettor to dispense all of the conjugate solution. Incubating the Sample 14. Close the sample tube, gently invert it four to five times and place it in the SNAP Reader incubator. 15. Press 1 to start the VetTest analyzer s five-minute automatic timer or press 2 to time the five-minute incubation manually. IMPORTANT If you are performing only a T 4 test on the sample, press E at the Insert Slide screen to return to the VetTest main menu while the sample is incubating. If you are performing an additional analysis on this sample on other IDEXX VetLab analyzers, you can proceed with that testing while the T 4 sample is incubating. Adding the Sample to the SNAP Device 16. After the five-minute incubation, remove the sample tube from the incubator. 17. Place the SNAP T 4 device on a flat surface and pour the entire contents of the sample tube into the sample well. 18. When the sample FIRST appears in the activation circle, push down firmly on the activator button until it is flush with the device body. 15

26 IDEXX SNAP Reader Analyzer Operator s Manual Reading the SNAP Device 19. Immediately insert the SNAP T 4 device into the SNAP Reader with the sample well end inserted first. Keep the device horizontal at all times. Note: Do not remove the device from the SNAP Reader during analysis. 20. Remove the SNAP T 4 device from the SNAP Reader when prompted and press any key to continue. T 4 results will print automatically. Note: If other tests were previously run on any IDEXX VetLab analyzer, results can be combined by following the prompts on the VetTest screen. Standard Curve Information T 4 standard curve information is laser-etched onto each SNAP T 4 device and is read automatically. If necessary, standard curve information can also be entered manually by following instructions on the VetTest screen. The standard curve number and lot number can be found on the SNAP T 4 kit label. Important! Insert the sample well end of the SNAP device into the SNAP Reader. sample well reference reagent test reagent activation circle activator 16

27 4 Calibration and Maintenance Procedures 4.1 SNAP Reader Calibration Procedure The Calibration SNAP (CalSNAP ) test should be run daily on the SNAP Reader analyzer. It calibrates the analyzer and performs self-diagnostic checks, confirming that the SNAP Reader analyzer is working properly. You should also run your Calibration SNAP test: When the analyzer is first installed If the analyzer is moved or jarred severely When you want to ensure the analyzer is working properly Note: When not in use, store the CalSNAP in its black case. Running the Calibration SNAP Test 1. Turn on the SNAP Reader analyzer and allow it to warm up for 30 minutes before running a CalSNAP test. 2. Be sure that the VetTest analyzer is turned on and is displaying the Main menu on its touch screen. A SNAP icon should be displayed. 3. If the time and date on the SNAP Reader analyzer is incorrect, press 6 on the VetTest analyzer s Main menu to reinitialize the SNAP Reader analyzer. 4. Insert the CalSNAP into the SNAP Reader analyzer. NOTE: The SNAP Reader analyzer performs a series of internal diagnostics, which are completed in about 90 seconds. The SNAP Reader analyzer s LCD display and the VetTest analyzer s touch screen both show the results of the CalSNAP procedure. If the analyzer passes its diagnostic checks, the message Calibration OK appears on the SNAP Reader analyzer s LCD display, and the message SNAP Reader Calibration Complete appears on the VetTest touch screen. If the analyzer does not pass its diagnostic checks, the SNAP Reader analyzer s LCD display shows the message Cal Error along with an error code. Write down the error code number and contact IDEXX Customer Support. Note: See Chapter 5: Messages and Error Codes for error code explanations. 4.2 General Maintenance Procedures Calibration SNAP device Sample well Insert into the SNAP Reader analyzer sample well end first Powering Off The SNAP Reader analyzer should be turned off nightly using the on/off switch located on the back of the analyzer. WARNING: Always disconnect the power cord from the electrical outlet before cleaning. 17

28 IDEXX SNAP Reader Analyzer Operator s Manual Case Cleaning Clean the outside of the SNAP Reader analyzer with a damp (not wet), lint-free cloth. A mild liquid soap will remove dirt or stains. Do not use any of the following near the instrument: organic solvents, ink markers, sprays containing volatile liquids, insecticides, polish, room freshener, aerosols or propellant solutions. If you need to disinfect the unit, do not use an aerosol disinfectant. Aerosols may damage the internal components. Using a lint-free cloth, apply a liquid disinfectant to the analyzer. Take care not to spill any samples, chemicals, cleaning agents, water, solvents or other fluids on the analyzer. Liquids and harsh abrasives should be kept away from openings and the LCD screen or LCD display Incubator Cleaning If fluids are spilled in the incubator, wipe the inside of the incubator with a damp, lintfree cloth. Never pour any fluids into the incubator SNAP Entry Port Cleaning If anything is spilled in the entry port, wipe it immediately with a damp, lint-free cloth. Confine wiping and cleaning of the entry port to the bottom, side and rear surfaces, taking care not to come in contact with the lens on the top of the port. If any fluid cannot be reached, call IDEXX Customer Support for service. 18

29 5 Messages and Error Codes The SNAP Reader analyzer has no user-serviceable or -adjustable components. Do not attempt to disassemble, modify or make any adjustments to the analyzer as serious damage to the precision components could occur. Any disassembly or analyzer modification without the explicit direction of IDEXX Customer Support may void the warranty. 5.1 VetTest Analyzer Displayed or Printed Messages SNAP Calibration Entry. Enter standard curve number from SNAP device package. Cause: The SNAP Reader analyzer has not correctly identified the standard curve number on the SNAP test. Action: Enter the information using the VetTest keypad or external keyboard. The calibration information is a sequence of numbers found on the SNAP test package or on the kit label itself. SNAP Message: # Causes: The automatic self-test of the SNAP Reader analyzer has detected a system failure while running diagnostics on the CalSNAP. Communication problems Bad SNAP test Action: Write down the error code number displayed on the VetTest screen and follow the suggestions at the bottom of the VetTest screen. 5.2 SNAP Reader Analyzer Error Codes and LCD Display Messages SNAP Reader Analyzer Error Numbers and Codes ### Code Category Specific 1 DIAG ERROR Calibration Error 2 DIAG ERROR Calibration Error 3 DIAG ERROR Calibration Error 10 DIAG ERROR Hardware Error Unable to recognize CalSNAP despite successful Vericode read Recognizes CalSNAP, but unable to read Vericode Unable to set calibrator autoexposure; image may be too dark Unable to identify flash chip properly 100 DIAG ERROR Hardware Error Incubator diagnostic failed 1000 DIAG ERROR Hardware Error Camera diagnostic failed 1010 DIAG ERROR Hardware Error Combined Error 1000 and DIAG ERROR Hardware Error Combined Error 1000 and DIAG ERROR Hardware Error Combined Error 1000, 100 and 10 19

30 IDEXX SNAP Reader Analyzer Operator s Manual SNAP Reader Analyzer Error Numbers and Codes ### Code Category Specific 200 INSUF DEV Development Problem 202 NO LDET Development Problem 203 NO CHANGE Development Problem 204 DEV REMOVED Development Problem 300 UNSUP CURV Incompatible Data 301 UNSUP SPE Incompatible Data 302 BAD TYPE Incompatible Data Reference stripe did not achieve minimum response during development time Failure to find reference and/or sample stripes on device No change in reference stripe during development time Device removed early (during analysis) Attempt to calculate results for a curve that does not exist in this software Attempt to calculate results for a species that is not supported by this software The device that was inserted did not match the test chosen 303 UNSUPP Incompatible Data The device is older than 04/01/ CALC ERR Computation Error 401 CALC ERR Computation Error No result calculation possible: math error No result calculation possible: no Vericode 500 NO MATCH Hardware Error No results available 501 SAVE ERR Hardware Error Unable to record results 502 RTRV ERR Hardware Error Unable to retrieve results 600 DIAG ERROR Calibration Error Bad parameters 601 DIAG ERROR Calibration Error No device detected 602 DIAG ERROR Calibration Error Device not a CalSNAP 603 DIAG ERROR Calibration Error Cannot read Vericode (can no longer happen post version 3.66) 604 DIAG ERROR Calibration Error DM<LM while setting AGC 605 DIAG ERROR Calibration Error Proper AGC setting not found 606 DIAG ERROR Calibration Error DM<LM while setting clamp 607 DIAG ERROR Calibration Error Proper clamp setting not found 608 DIAG ERROR Calibration Error No proper settings found 609 DIAG ERROR Calibration Error Bad CalSNAP curve type 20

31 Chapter 5: Messages and Error Codes 5.3 Troubleshooting (when no messages are displayed/printed) SNAP Reader analyzer does not communicate with the other analyzers. Symptoms: No SNAP icon appears on the VetTest screen. There is no prompt from the VetTest analyzer to run a SNAP test. No results are passed to the VetTest analyzer. The SNAP Reader analyzer s LCD does not show the current time or date. Possible Causes: Cables and plugs are not properly connected to all the analyzers. Power is not being supplied to the SNAP Reader analyzer. The test is not supported for the species selected. Recommended Actions: If other analyzers are connected to the VetTest analyzer, check to see that their icons are present. Ensure that communication with any of the other analyzers is working properly. Reinitialize remote analyzers by pressing 6 on the VetTest analyzer s Main menu. Check that all connections have been made according to the installation instructions for your particular configuration. Check the SNAP test kit s package insert to confirm the species is supported for the selected SNAP test. If the problem persists, call IDEXX Customer Support. SNAP Reader analyzer does not recognize SNAP test is inserted. Symptoms: There is no audible signal when the SNAP test is inserted. The red light does not flash when the SNAP test is inserted. Possible Causes: The SNAP test is not properly inserted into the SNAP entry port. The SNAP Reader analyzer is not functioning normally. Recommended Actions: Ensure that the SNAP test is fully inserted into the SNAP entry port. Insert the CalSNAP into the SNAP entry port and allow the instrument to complete the self-test. If it passes, repeat the test with a new SNAP test and ensure full insertion into the SNAP entry port. If the self-test fails (write down the error code), or if the problem persists, call IDEXX Customer Support. 21

32 IDEXX SNAP Reader Analyzer Operator s Manual SNAP Reader analyzer detects multiple insertions of the SNAP test during an analysis. Symptoms: The incubator light is accompanied by multiple SNAP insertion and removal auditory signals. An error code is displayed on both the SNAP Reader and VetTest analyzer screens. Possible Causes: The SNAP test is not properly inserted into the SNAP entry port. The SNAP Reader analyzer is not functioning normally. Recommended Actions: Ensure that the SNAP test is fully inserted into the SNAP entry port. Insert the CalSNAP into the SNAP entry port and allow the instrument to complete the self-test. If it passes, repeat the test with a new SNAP test. If the self-test fails (write down the error code), or if the problem persists, call IDEXX Customer Support. Power is not properly applied to the SNAP Reader analyzer. Symptoms: No messages on LCD screen or LCD display/no LCD light. The LED light in front of the incubator is not lit; neither orange nor green. There is no communication to the VetTest analyzer. The SNAP Reader analyzer icon doesn t appear on the VetTest analyzer s Main menu. Possible Causes: On/off switch is in off ( Ø ) position. Power connections may be seated improperly. Switchable outlets may be turned off. Power pack is not operating. The SNAP Reader analyzer is not functioning properly. Recommended Actions: Turn on the SNAP Reader analyzer by pressing the on/off switch to the on (1) position. Check all the power connections to the SNAP Reader analyzer. Ensure that any power strips or switchable electrical outlets are in the on position. If the problem persists, call IDEXX Customer Support. 22

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