Huntingtin Antibody AbVantage Pack

Transcription

1 Huntingtin Antibody AbVantage Pack Produced in Rabbit Catalog No. A3-034A NP_ CONTENTS Rabbit anti-huntingtin A302-82A Rabbit anti-huntingtin A302-83A WB, IP REACTIVITY STORAGE / SHELF LIFE 2-8 C / year from date of receipt NOTE (FAX) info@bethyl.com

2 Huntingtin Antibody Rabbit Polyclonal Catalog No. A302-82A NP_ REACTIVITY TESTED PRESUMED REACTIVITY ISOTYPE AMOUNT STORAGE/SHELF LIFE BUFFER PRODUCTION PROCEDURES WB, IP Based on 00% sequence identity, this antibody is predicted to react with Horse, Orangutan, Rhesus Monkey, Gorilla, Chimpanzee, White-tufted-ear marmoset, Crab-eating macaque, Gray short-tailed opossum, Small-eared galago and Northern white-cheeked gibbon. IgG 00 µl at 0.2 mg/ml 2-8 C / year from date of receipt Tris-buffered Saline with 0.% BSA containing 0.09% Sodium Azide Antibody was affinity purified using an epitope specific to Huntingtin immobilized on solid support. The epitope recognized by A302-82A maps to a region between residue 50 and 200 of human Huntingtin using the numbering given in entry NP_ (GeneID ). Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of.4 equals.0 mg of IgG. APPLICATION NOTES ADDITIONAL INFO Western Blot :2,000 to :0,000 Immunoprecipitation 2 to 5 µg/mg lysate Validation by IP/Western Blot was performed using 3-8% tris-acetate gel and ReliaBLOT Reagents (Cat. No. WB20). Use the link above to view SDS, a current list of citations, and other product specific information.

3 Huntingtin Antibody: A302-82A Detection of Huntingtin by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 5 and 50 µg for WB; mg for IP, 20% of IP loaded) and 293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti- Huntingtin antibody A302-82A used for WB at 0.04 µg/ml (A) and 0.4 µg/ml (B) and used for IP at 3 µg/mg lysate. Huntingtin was also immunoprecipitated by rabbit anti-huntingtin antibody A302-83A, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 0 seconds (A) and 3 seconds (B). 2

4 Huntingtin Antibody Rabbit Polyclonal Catalog No. A302-83A NP_ REACTIVITY TESTED PRESUMED REACTIVITY ISOTYPE AMOUNT STORAGE/SHELF LIFE BUFFER PRODUCTION PROCEDURES IP Based on 00% sequence identity, this antibody is predicted to react with Rhesus Monkey, Gorilla, Chimpanzee, Crab-eating macaque and Northern white-cheeked gibbon. IgG 00 µl at mg/ml 2-8 C / year from date of receipt Tris-citrate/phosphate buffer, ph 7 to 8 containing 0.09% Sodium Azide Antibody was affinity purified using an epitope specific to Huntingtin immobilized on solid support. The epitope recognized by A302-83A maps to a region between residue 2300 and 2350 of human Huntingtin using the numbering given in entry NP_ (GeneID ). Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of.4 equals.0 mg of IgG. APPLICATION NOTES ADDITIONAL INFO Western Blot Immunoprecipitation Not Recommended 2 to 5 µg/mg lysate Validation by IP/Western Blot was performed using 3-8% tris-acetate gel and ReliaBLOT Reagents (Cat. No. WB20). Use the link above to view SDS, a current list of citations, and other product specific information.

5 Huntingtin Antibody: A302-83A Detection of Huntingtin by Western Blot of Immunoprecipitates. Samples: Whole cell lysate ( mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-huntingtin antibody A302-83A used for IP at 3 µg/mg lysate. Huntingtin was also immunoprecipitated by rabbit anti-huntingtin antibody A302-82A, which recognizes an upstream epitope. For blotting immunoprecipitated Huntingtin, A302-82A was used at µg/ml,. Detection: Chemiluminescence with an exposure time of 3 seconds. 2