FAK phospho S732 Antibody, Rabbit Polyclonal Antibody

Transcription

1 GenWay Biotech, Inc Nancy Ridge Drive San Diego, CA Tel: (858) Fax: (858) FAK phospho S732 Antibody, Rabbit Polyclonal Antibody Catalog Number: Related Product Names: - FAK antibody; FAK; 5747; PTK2; ; Q05397; FADK; FAK; FAK1; pp125fak;; FAK phospho S732 - Rabbit polyclonal to FAK ( phospho S732 ); 5747; PTK2; ; Q05397; FADK; FAK; FAK1; pp125fak;; Focal adhesion kinase 1 - Gene Information - Information in yellow represents specific gene information and does not necessarily represent specific product details. For more information please contact [email protected]. Gene Name: FAK Gene Name Synonym: 5747; PTK2; ; Q05397; FADK; FAK; FAK1; pp125fak; Gi #: N/A NCBI Acc #: NP_ Swiss Prot Acc #: Q05397 Length (aa): N/A Mol. Weight (Da): Chrom Location: N/A Isotype: IgG Immunogen: Synthetic peptide (Human) derived from the region of human Focal Adhesion Kinase that contains serine 732. The sequence is conserved in mouse, rat and chicken. Antigen Species: Human Specificity: The antibody also reacts with FRNK. Positive Control: Cdk5 +/- and cdk5 -/- mouse brain lysates Target: FAK Localization: Cytoplasmic, localized to focal adhesions. Purification Note: Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using (i) a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated Focal Adhesion Kinase enzyme and (ii) a generic serine phosphorylated peptide to remove antibody that is reactive with phosphoserine, irrespective of the sequence. The final product is generated by affinity chromatography using a Focal Adhesion Kinase-derived peptide that is phosphorylated at serine 722. Storage Buffer: Preservative: 0.05% Sodium Azide; Constituents: 50% Glycerol, PBS, 1mg/ml BSA (IgG, protease free). ph 7.3 Application Note: WB: Use at a concentration of µg/ml. Predicted molecular weight: 125 kda. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user. Cellular Localization: Cytoplasmic, localized to focal adhesions. Source/Host: Rabbit Purity/Purification: Immunogen affinity purified Clonality: Polyclonal Crossreactivity: Cross-reacts with Rat and Mouse. Expected to cross-react with Chicken (100% identity with immunogen), Human (100% identity with immunogen), Xenopus laevis (100% identity with immunogen) due to sequence homology. Not yet tested in other species.

2 Shipping: Products may be shipped on ice pack or dry ice. APPLICATIONS for FAK ANTIBODY: WB: Tested TESTING: (secondary reagents and protocols) Not Available FAK ANTIBODY TARGET DESCRIPTION: Synonym Names for FAK antibody: FAK; 5747; PTK2; ; Q05397; FADK; FAK; FAK1; pp125fak;; Rabbit polyclonal to FAK ( phospho S732 ); 5747; PTK2; ; Q05397; FADK; FAK; FAK1; pp125fak;; Focal adhesion kinase 1 Focal Adhesion Kinase is a 125 kda non-receptor protein tyrosine kinase that plays a key role in signalling by growth factors, extracellular matrix and stress signals. Indeed, Focal Adhesion Kinase plays a central role in cell spreading, differentiation, migration, cell death and acceleration of the G1 to S phase transition of the cell cycle. Cyclin-dependent kinase-5 (Cdk5), a serine/threonine kinase and an important regulator in brain development, has been found to be essential for phosphorylation at serine 732. In the context of the Focal Adhesion Kinase- Related Non-Kinase (FRNK), the c-terminal non-kinase domain of Focal Adhesion Kinase that acts as a dominant negative inhibitor of Focal Adhesion Kinase signalling, other serine kinases appear to readily phosphorylate this same site. The functional significance of phosphorylation on serine 732 is yet to be determined. Function: Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Plays a potential role in oncogenic transformations resulting in increased kinase activity. Catalytic Activity: ATP + a [protein]-l-tyrosine = ADP + a [protein]-l-tyrosine phosphate. Subunit: Interacts with CAS family members and with GIT1, SORBS1 and BCAR3. Interacts with RGNEF and SHB (By similarity). Interacts with TGFB1I1. Subcellular Location: Cell junction, focal adhesion. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Note=Constituent of focal adhesions. Tissue Specificity: Expressed in all organs tested, in lymphoid cell lines, but most abundantly in brain. Domain: The first Pro-rich domain interacts with the SH3 domain of CRK-associated substrate (BCAR1) and CASL (By similarity). Domain: The carboxy-terminal region is the site of focal adhesion targeting (FAT) sequence which mediates the localization of FAK1 to focal adhesions. Ptm: Phosphorylated on 6 tyrosine residues upon activation. Similarity: Belongs to the protein kinase superfamily. Tyr protein kinase family. FAK subfamily. Similarity: Contains 1 FERM domain. Similarity: Contains 1 protein kinase domain. FAK phospho S732 reacts with cross-reacts with rat and mouse. expected to cross-react with chicken (100% identity with immunogen), human (100% identity with immunogen), xenopus laevis (100% identity with immunogen) due to sequence homology. not yet tested in other species..

3 OMIM: ; gene. [NCBI / EBI] Pathways: KEGG pathway: Axon guidance KEGG pathway: ErbB signaling pathway KEGG pathway: Focal adhesion KEGG pathway: Leukocyte transendothelial migration KEGG pathway: Regulation of actin cytoskeleton KEGG pathway: Small cell lung cancer KEGG pathway: VEGF signaling pathway Reactome Event:Apoptosis PRODUCT INTERACTIONS: FAK phospho S732 interacts with Paxillin (Phospho-Tyr118), IgG (GenWay FAK phospho S732 interacts with Paxillin (Phospho-Tyr31), IgG (GenWay FAK phospho S732 interacts with Paxillin (Ab-118), IgG (GenWay FAK phospho S732 interacts with Paxillin (Ab-31), IgG (GenWay FAK phospho S732 interacts with Paxillin (Phospho-Tyr118) Antibody Blocking Peptide, Peptide (GenWay FAK phospho S732 interacts with Paxillin (Phospho-Tyr31) Antibody Blocking Peptide, Peptide (GenWay FAK phospho S732 interacts with Paxillin [PXC-10], IgG (GenWay FAK phospho S732 interacts with Paxillin phospho Y31, IgG (GenWay FAK phospho S732 interacts with Paxillin phospho Y118, IgG (GenWay FAK phospho S732 interacts with Phospho-Paxillin (py31), IgG (GenWay FAK phospho S732 interacts with Paxillin, IgG (GenWay FAK phospho S732 interacts with Paxillin Phospho (py181), IgG (GenWay FAK phospho S732 interacts with Recombinant Human Paxillin, Protein (GenWay Catalog #: ). FAK phospho S732 interacts with SRC, Protein (GenWay FAK phospho S732 interacts with SRC, IgG (GenWay FAK phospho S732 interacts with SRC, IgG (GenWay FAK phospho S732 interacts with Src (Phospho-Tyr418), IgG (GenWay FAK phospho S732 interacts with Src (Phospho-Tyr529), IgG (GenWay FAK phospho S732 interacts with Src (Ab-418), IgG (GenWay FAK phospho S732 interacts with Src (Ab-529), IgG (GenWay FAK phospho S732 interacts with Src(Phospho-Tyr418) Antibody Blocking Peptide, Peptide (GenWay FAK phospho S732 interacts with Src (Phospho-Tyr529) Antibody Blocking Peptide, Peptide (GenWay FAK phospho S732 interacts with Src Mouse, Protein (GenWay FAK phospho S732 interacts with SRC, IgG (GenWay FAK phospho S732 interacts with SRC phospho Y418, IgG (GenWay FAK phospho S732 interacts with Src (py529), IgG (GenWay FAK phospho S732 interacts with Src, IgG (GenWay FAK phospho S732 interacts with Src, IgG (GenWay FAK phospho S732 interacts with Proto-Oncogene Protein Tyrosine Kinase SRC (SRC) Rabbit anti-mouse Polyclonal (N-Terminus) Antibody, IgG (GenWay FAK phospho S732 interacts with Cellular tumor antigen p53, IgG (GenWay FAK phospho S732 interacts with Cellular tumor antigen p53, IgG (GenWay FAK phospho S732 interacts with p53 Human, Protein (GenWay FAK phospho S732 interacts with p53m Human, Protein (GenWay FAK phospho S732 interacts with p53 [SP5], IgG (GenWay FAK phospho S732 interacts with p53 phospho S15, IgG (GenWay FAK phospho S732 interacts with p53 [DO-13], IgG (GenWay FAK phospho S732 interacts with p53 [DO-7], IgG (GenWay FAK phospho S732 interacts with p53 [Pab240], IgG (GenWay FAK phospho S732 interacts with p53 [D01], IgG (GenWay FAK phospho S732 interacts with p53 [3F301], IgG (GenWay FAK phospho S732 interacts with p53 [DO-7] (ready-to-use), IgG (GenWay FAK phospho S732 interacts with p53 [BP53-12], IgG (GenWay FAK phospho S732 interacts with I-kappaB-alpha (Phospho-Ser32/Ser36), IgG (GenWay Catalog #: ). FAK phospho S732 interacts with p53 (Phospho-Ser15), IgG (GenWay FAK phospho S732 interacts with p53 (Phospho-Ser46), IgG (GenWay FAK phospho S732 interacts with p53 (Phospho-Ser315), IgG (GenWay FAK phospho S732 interacts with p53 (Ab-15), IgG (GenWay FAK phospho S732 interacts with p53 (Ab-46), IgG (GenWay FAK phospho S732 interacts with p53 (Ab-315), IgG (GenWay

4 FAK phospho S732 interacts with p53 (Phospho-Ser15) Antibody Blocking Peptide, Peptide (GenWay FAK phospho S732 interacts with p53 (Phospho-Ser46) Antibody Blocking Peptide, Peptide (GenWay FAK phospho S732 interacts with p53 (Phospho-Ser315) Antibody Blocking Peptide, Peptide (GenWay FAK phospho S732 interacts with TP53 (tumor protein p53 (Li-Fraumeni syndrome)), IgG (GenWay FAK phospho S732 interacts with p53 (a.a ), IgG (GenWay FAK phospho S732 interacts with p53 (a.a ), IgG (GenWay FAK phospho S732 interacts with Mouse p53, Wild Type, IgG (GenWay FAK phospho S732 interacts with Human p53 Oncogene, IgG (GenWay FAK phospho S732 interacts with p53 [B20.1 (BP )] antibody (ready-to-use), IgG (GenWay FAK phospho S732 interacts with p53 AIP1, IgG (GenWay FAK phospho S732 interacts with p53 [BP53-12] (FITC), IgG (GenWay FAK phospho S732 interacts with p53 [DO-2], IgG (GenWay FAK phospho S732 interacts with p53 [DO-1], IgG (GenWay FAK phospho S732 interacts with P53, IgG (GenWay FAK phospho S732 interacts with p53 (aa20-25), IgG (GenWay FAK phospho S732 interacts with P53 [SP5] (ready-to-use), IgG (GenWay FAK phospho S732 interacts with p53 [DO-1], IgG (GenWay FAK phospho S732 interacts with p53 [B449], IgG (GenWay FAK phospho S732 interacts with p53 [Pab240], IgG (GenWay FAK phospho S732 interacts with p53 [Pab1801], IgG (GenWay FAK phospho S732 interacts with p53 [Pab1801], IgG (GenWay FAK phospho S732 interacts with p53 [DO-2], IgG (GenWay FAK phospho S732 interacts with p53 (N-Term), IgG (GenWay FAK phospho S732 interacts with p53 (C-Term), IgG (GenWay FAK phospho S732 interacts with Phospho p53 (ps6), IgG (GenWay FAK phospho S732 interacts with Phospho p53 (ps9), IgG (GenWay FAK phospho S732 interacts with P53 Phospho (ps392), IgG (GenWay FAK phospho S732 interacts with p53 Phospho (ps46), IgG (GenWay FAK phospho S732 interacts with p53 Acetyl (K373), IgG (GenWay FAK phospho S732 interacts with p53 Phospho (ps33), IgG (GenWay FAK phospho S732 interacts with p53 Phospho (ps392), IgG (GenWay FAK phospho S732 interacts with p53 Acetyl (K382), IgG (GenWay FAK phospho S732 interacts with Recombinant Human p53 GST-Tag, Protein (GenWay Catalog #: ). FAK phospho S732 interacts with Tumor Protein P53 (Li-Fraumeni Syndrome) (TP53) Rabbit anti- Human Polyclonal Antibody, IgG (GenWay FAK phospho S732 interacts with Tumor Protein P53 (Li-Fraumeni Syndrome) (TP53) Mouse anti- Human Monoclonal Antibody, IgG (GenWay FAK phospho S732 interacts with Tumor Protein P53 (Li-Fraumeni Syndrome) (TP53) Mouse anti- Human Monoclonal (pser392) (FP3.2) Antibody, IgG (GenWay FAK phospho S732 interacts with RABBIT ANTI HUMAN p53, IgG (GenWay FAK phospho S732 interacts with MOUSE ANTI p53 MUTANT, IgG (GenWay FAK phospho S732 interacts with MOUSE ANTI HUMAN p53 (aa20-25):fitc, IgG (GenWay Catalog #: ).

5 Order Confirmation: Sales order confirmations are sent out upon the receipt of all orders. Please contact GenWay if you do not receive a confirmation within 1 business day of submitting your order. Precautions: FAK antibody is for in vitro research use only. Not for use in diagnostics or therapeutic procedures. Important Notes: During shipment, small volumes of FAK antibody vial. For products with volumes of 200 µl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container s cap. Actual concentration, volume and quantity will be printed on the vial's label. Please refer to the vials label for this information. Copyright: This GenWay TDS is copyrighted. This datasheet is produced based partially on data from Swiss- Prot/TrEMBL and NCBI. To better serve our clients with everything we know about FAK antibody, all related information, articles, resources about FAK antibody are being stored on our online database. Let us know if you have questions regarding this product. Disclaimer: For documents and software available from this server, GenWay neither warrants nor assumes any legal liability or responsibility for the accuracy, completeness or utility of any information, product or process disclosed. GenWay Biotech, Inc Nancy Ridge Drive, San Diego, CA Tel: (858) Fax: (858) [email protected] GenWay Biotech, Inc. - All Rights Reserved