Polígono de Pocomaco Calle 1 Parcela G La Coruña

Transcription

1 MATERIAL DE LABORATORIO INSTRUMENTACIÓN Y PRODUCTOS QUÍMICOS Polígono de Pocomaco Calle 1 Parcela G La Coruña Teléfono: Fax: [email protected] Web: TARIFA 2013 THERMOSTABLE DNA POLYMERASES AND KITS PCR/ REAL-TIME PCR KITS dntps/ntps PCR ENHANCING REAGENTS POLYMERASES REVERSE TRANSCRIPTASES TRANSCRIPTION KITS REVERSE TRANSCRIPTION KITS LIGASES NUCLEASES DNA-MODIFYING AND RELATED PROTEINS MOLECULAR WEIGHT MARKERS SPECIALITY REAGENTS BUFFERS & CHEMICALS DNA PURIFICATION SYSTEMS RNA PURIFICATION SYSTEMS EMULSION KITS RESTRICTION ENDONUCLEASES NEW PRODUCTS: Fast SG qpcr Master Mix Perfect TM Color Protein Ladder PCR water (Molecular Biology Grade) Nuclease-free water, not DEPC treated (Molecular Biology Grade) Random Hexamer Primers SimplySafe TM Agarose tablets (Molecular Biology Grade) NXT Taq PCR Kit

2 Super Claro Agarose (Molecular Biology Grade) Low Melting Agarose (Molecular Biology Grade) High Resolution Agarose (Molecular Biology Grade) Agarose (Molecular Biology Grade) THERMOSTABLE DNA POLYMERASES AND KITS: Taq DNA Polymerases Cat.No. Package Kit Cat.No. Kit Taq DNA Polymerase 5.0u/μl E u 28,89 EK ,67 Top quality general application Taq DNA polymerase E u 64,44 EK ,33 E u 112,22 EK ,11 E u 555,56 EK ,00 Color Taq DNA Polymerase 1.0u/μl E u 28,89 EK ,67 Dyes-traced top quality general application Taq DNA polymerase E u 64,44 EK ,33 E u 112,22 EK ,11 E u 555,56 EK ,00 Native Taq DNA Polymerase 5.0u/μl E u 34,44 EK ,22 Top quality general application native Taq DNA polymerase E u 76,67 EK ,56 E u 135,56 EK ,44 E u 666,67 EK ,11 OptiTaq DNA Polymerases Kit OptiTaq DNA Polymerase 5.0u/μl E u 34,44 EK ,22 Proofreading blend of DNA polymerases for any template and long PCR E u 76,67 EK ,56 E u 135,56 EK ,44 E u 666,67 EK ,11 Color OptiTaq DNA Polymerase 1.0u/μl E u 34,44 EK ,22 Dyes-traced proofreading blend of DNA polymerases for any template and long PCR E u 76,67 EK ,56 E u 135,56 EK ,44 E u 666,67 EK ,11 Hot Start Taq DNA Polymerases Kit Perpetual Taq DNA Polymerase 2.5u/μl E u 75,56 EK ,33 Monoclonal antibody automatic hot start PCR system E u 171,11 EK ,00 E u 290,00 EK ,89 E u 1.376,67 EK ,11 Color Perpetual Taq DNA Polymerase 1.0u/μl E u 75,56 EK ,33 Dyes-traced monoclonal antibody automatic hot start PCR system E u 171,11 EK ,00 E u 290,00 EK ,89 E u 1.376,67 EK ,11 Hot Start OptiTaq DNA Polymerases Kit Perpetual OptiTaq DNA Polymerase 2.5u/μl E u 87,78 EK ,56 Monoclonal antibody automatic hot start proofreading PCR system E u 198,89 EK ,78 E u 347,78 EK ,67

3 E u 1.653,33 EK ,78 Color Perpetual OptiTaq DNA Polymerase 1.0u/μl E u 87,78 EK ,56 Dyes-traced monoclonal antibody automatic hot start proofreading PCR system E u 198,89 EK ,78 E u 347,78 EK ,67 E u 1.653,33 EK ,78 THERMOSTABLE DNA POLYMERASES AND KITS: Amplus DNA Polymerase Cat.No. Package Kit Cat.No. Kit Amplus DNA Polymerase 5.0u/μl E u 16,67 EK ,11 Amplifies large genomic DNA fragments from 3 kb to over 25 kb and E u 76,67 EK ,56 episomal fragments up to 40 kb Hybrid DNA Polymerase Cat.No. Package Kit Cat.No. Kit Hybrid DNA Polymerase 2.0u/μl E u 60,00 EK ,33 Extremely thermostable DNA polymerase, enabling efficient high fidelity PCR E u 234,44 EK ,33 of genomic targets up to 12 kb and episomal targets up to 20 kb Pfu DNA Polymerases Kit PfuPlus! DNA Polymerase 5.0u/μl E u 56,67 EK ,00 Top quality special application PfuPlus! DNA polymerase E u 221,11 EK ,00 E u 1.,00 EK ,67 Color PfuPlus! DNA Polymerase 1.25u/μl E u 56,67 EK ,00 Dyes-traced top quality special application PfuPlus! DNA polymerase E u 221,11 EK ,00 E u 1.,00 EK ,67 Thermus DNA Polymerases Tfl DNA Polymerase E u 55,56 EK ,33 Native Thermus flavus DNA polymerase E u 220,00 EK ,89 Tth DNA Polymerase E u 50,00 EK ,33 Native Thermus thermophilus DNA polymerase E u 200,00 EK ,89 All thermostable DNA Polymerases are also available as kits, which include a DNA Polymerase and a mix of carefully tested and calibrated ultrapure dntps. PCR/ REAL-TIME PCR KITS Cat.No. Package NXT Taq PCR Kit NEW E reactions 44,44 E reactions 86,67 E reactions 177,78 Direct Blood PCR Kit E reactions 64,44 E reactions 257,78 Taq PCR Master Mix (2x) E reactions 35,56 E reactions 66,67 E reactions 144,44 Perpetual Taq PCR Master Mix (2x) E reactions 80,00 E reactions 155,56 E reactions 322,22 SG qpcr Master Mix (2x) E reactions 47,78 Real-time PCR Kit E reactions 91,11 E reactions 411,11 SG qpcr Master Mix (2x), plus ROX Solution E reactions 51,11 Real-time PCR Kit with ROX Solution E reactions 98,89

4 E reactions 433,33 Blank qpcr Master Mix (2x) E reactions 46,67 Real-time PCR Kit E reactions 88,89 E reactions 400,00 Blank qpcr Master Mix (2x), plus ROX Solution E reactions 50,00 Real-time PCR Kit with ROX Solution E reactions 96,67 E reactions 422,22 Fast SG qpcr Master Mix (2x) NEW E reactions 53,33 Real-time PCR Kit E reactions,00 E reactions 472,22 dntps/ntps Cat.No. Package 10 mm Ultrapure dntps Set E x 200 μl 8,89 4 x 10 mm (datp, dctp, dgtp, dttp) E x 1 ml 44,44 mm Ultrapure dntps Set E x 200 μl 55,56 4 x mm (datp, dctp, dgtp, dttp) E x 1 ml 261,11 mm Ribo-Nucleotides Set E x 200 μl 38,89 4 x mm (ATP, CTP, GTP, UTP) E x 1 ml 166,67 PCR ENHANCING REAGENTS: Gp 32 Protein (single-stranded binding protein of T4 bacteriophage) E µg 60,00 E µg 250,00 Single-stranded DNA Binding Protein (E.coli) E µg 50,00 E µg 200,00 Taq Single-stranded DNA Binding Protein (Thermus aquaticus) E µg 50,00 E µg 200,00 POLYMERASES: DNA Polymerase Alpha (Human) E u 300,00 E u 1.022,22 DNA Polymerase Gamma (Human) E u temporary not E u available DNA Polymerase Beta (Human) E u 233,33 E u 800,00 Bst DNA Polymerase (Large Fragment, exo - ) E u 13,33 E u 53,33 DNA Polymerase I E u 50,00 E u 200,00 Klenow Fragment E u 50,00 E u 200,00 Klenow Fragment exo - E u 50,00 E u 200,00 Poly (A) Polymerase E u 55,56 E u 220,00 T4 DNA Polymerase E u 60,00 E u 240,00 T7 RNA Polymerase E u 75,56 E u 250,00 Terminal Deoxynucleotidyl Transferase E u 55,56 E u 220,00

5 REVERSE TRANSCRIPTASES: AMV Reverse Transcriptase Cloned E u 60,00 E u 240,00 AMV Reverse Transcriptase Native E u 75,56 E u 302,22 HIV Reverse Transcriptase E u 300,00 E u 1.200,00 Tth Reverse Transcriptase E u 80,00 TRANSCRIPTION KITS: T7 transcription kit E E u 320,00 Cat.No. Package 25x 25 µl react 111,11 50x 25 µl E react 200,00 REVERSE TRANSCRIPTION KITS: dart RT kit E E dart RT-PCR kit E E OneStep RT-PCR kit E E LIGASES: 25 reactions 72,22 reactions 265,56 25 reactions 83,33 reactions 305,56 25 reactions,00 reactions 366,67 DNA Ligase (E.coli) E u 125,56 E u 500,00 T4 DNA Ligase E u 60,00 E u 240,00 Taq DNA Ligase (Thermus aquaticus) E u 40,00 E u 160,00 NUCLEASES: RNase III (E.coli) E u 108,89 E u 435,56 Exonuclease III (E.coli) E u 50,00 E u 200,00 Lambda Exonuclease E u 50,00 E u 200,00 Mung Bean Nuclease E u 50,00 E u 200,00 Ribonuclease H (E.coli) E u 54,44 E u 220,00 RNase I E u 70,00 E u 280,00 S1 Nuclease E u 40,00 E u 156,67 DNA-MODIFYING AND RELATED PROTEINS:

6 Alkaline Phosphatase (Calf Intestine) E u 50,00 E u 200,00 Bacterial Alkaline Phosphatase (E.coli) E u 50,00 E u 200,00 Endocyclase N15 (Protelomerase N15) Unique! E u 98,89 E u 442,22 Rec A Protein (E.coli) E mg 68,89 E mg 272,22 SV40 Large Antigen Unique! E µg 188,89 E µg 722,22 SV40 DNA Replication Assay Kit Unique! E kit (20reactions) 388,89 T4 Polynucleotide Kinase E u 75,56 E u 302,22 Thermophilic Pyrophosphatase (Thermus aquaticus) E u 75,56 E u 302,22 MOLECULAR WEIGHT MARKERS: Cat.No. Package Perfect Plus bp DNA Ladder E loadings 61,11 E loadings 244,44 Perfect Plus 2 kb DNA Ladder E loadings 61,11 E loadings 244,44 Perfect Plus Molecular Weigt Quantitative DNA Ladder E loadings 61,11 E loadings 244,44 GENEFLO 625 DNA ladder, ROX Labeled E loadings 55,56 Fluorescent-labeled DNA ladder for use with ABI PRISM genetic analysers. E loadings 277,78 GENEFLO 625 DNA ladder, TAMRA Labeled E loadings 55,56 Fluorescent-labeled DNA ladder for use with ABI PRISM genetic analysers. E loadings 277,78 Perfect bp DNA Ladder E µg 50,00 E µg 200,00 Perfect -0bp DNA Ladder E µg 50,00 E µg 200,00 Perfect 1kb DNA Ladder E µg 40,00 E µg 160,00 Perfect Plus 1kb DNA Ladder E µg 43,33 E µg 173,33 Perfect Color Protein Ladder (prestained) NEW E loadings 66,67 SPECIALITY REAGENTS: Random Hexamer Primers NEW E μg 27,78 Agarose (Molecular Biology Grade) NEW E g 198,89

7 High Resolution Agarose (Molecular Biology Grade) NEW E g 183,33 Low Melting Agarose (Molecular Biology Grade) NEW E g 173,33 Super Claro Agarose (Molecular Biology Grade) NEW E g 158,89 Super low ethidium bromide background, high resolution Agarose tablets (Molecular Biology Grade) NEW E tablets 40,00 Each agarose tablet consists 0.5 g agarose. E tablets 158,89 Bovine Serum Albumin (Acetylated) E mg 50,00 E mg 200,00 Human COT DNA E mg 105,56 E mg 400,00 Uncut Human Placental DNA E mg 105,56 E mg 400,00 Ribonuclease Inhibitor E u 105,56 E u 422,22 TEV Protease E u 66,67 E u 600,00 Vivid Violet DNA / RNA Co - Precipitate E µl 50,00 SimplySafe NEW E ml 55,56 Non-toxic, non-mutagenic, non-carcinogenic alternative to ethiduim bromide (EtBr). BUFFERS & CHEMICALS RNase-free water E x 1.5 ml 20,00 E x 5.0 ml 34,44 PCR water (Molecular Biology Grade) NEW E x 1.5 ml 27,78 Ultrapure, sterile, deionized, double destiled and prealiquoted water. E x 1.5 ml 50,00 Nuclease-free water, not DEPC treated (Molecular Biology Grade) NEW E ml 20,00 50 x TAE (Molecular Biology Grade) NEW E ml 33,33 E l 55,56 5 x TBE (Molecular Biology Grade) NEW E l 22,22 E x 1 l, M EDTA ph 8.0 (Molecular Biology Grade) NEW E ml 17,78 E ml 27,78 E l 50,00 1 x TE ph 8.0 (Molecular Biology Grade) NEW E ml 17,78 E l 24,44 E x 1 l 111,11 DNA PURIFICATION SYSTEMS: Cat.No. Package GeneMATRIX PLASMID MINIPREP DNA Purification Kit E preps 50,00 Kit for isolation of high-purity plasmid DNA (1.5-4 ml bacterial culture). E preps 140,00 GeneMATRIX SHORT DNA CLEAN - UP DNA Purification Kit E preps 40,00 Kit for purification of short single-stranded and double-stranded DNA fragments after enzymatic reactions. E preps 143,33 GeneMATRIX PCR / DNA CLEAN - UP DNA Purification Kit E preps 43,33 Kit for purification of PCR products / DNA after enzymatic reactions. E preps 121,11 GeneMATRIX AGAROSE - OUT DNA Purification Kit E preps 51,11

8 Universal kit for purification of DNA from agarose gels. E preps 143,33 GeneMATRIX BASIC DNA Purification Kit E preps 53,33 Universal kit for purification of PCR products / DNA after enzymatic reactions, isolation of DNA from agarose gels and isolation of plasmid DNA from bacteria. GeneMATRIX TISSUE DNA Purification Kit E preps 82,22 Universal kit for purification of DNA from human and animal tissues. E preps 230,00 GeneMATRIX TISSUE & BACTERIAL DNA Purification Kit E preps 84,44 Kit for isolation of total DNA from human and animal tissues and bacteria. E preps 234,44 GeneMATRIX CELL CULTURE DNA Purification Kit Unique! E preps 82,22 Kit for purification of DNA from human and animal cell cultures. E preps 230,00 GeneMATRIX BONE DNA Purification Kit E preps 74,44 Kit for isolation of DNA from animal or human bones. E preps 136,67 GeneMATRIX QUICK BLOOD DNA Purification Kit E preps 87,78 Kit for quick purification of DNA from fresh or frozen blood. E preps 241,11 GeneMATRIX SOIL DNA Purification Kit E preps 105,56 Kit for purification of DNA from soil. E preps 205,56 GeneMATRIX STOOL DNA Purification Kit E preps 105,56 Kit for purification of DNA from stool. E preps 205,56 GeneMATRIX BACTERIAL & YEAST GENOMIC DNA Purification Kit E preps 81,11 Universal kit for isolation of total DNA from bacteria Gram +, Gram - and yeast. E preps 226,67 GeneMATRIX PLANT & FUNGI DNA Purification Kit Unique! E preps 93,33 Universal kit for purification of total DNA from plants, fungi and lichens. E preps 260,00 GeneMATRIX BIO-TRACE DNA Purification Kit E preps 54,44 Kit for purification of DNA from various samples for clinical and forensic analysis. E preps 201,11 GeneMATRIX SWAB-EXTRACT DNA Purification Kit E preps 40,00 Kit for purification of DNA from swabs for clinical and forensic analysis. E preps 147,78 GeneMATRIX FOOD-EXTRACT DNA Purification Kit E preps 54,44 Kit for purification of DNA from food. E preps 201,11 RNA PURIFICATION SYSTEMS: GeneMATRIX HUMAN BLOOD RNA Purification Kit E preps 58,89 Kit for isolation of total RNA from fresh human blood. GeneMATRIX UNIVERSAL DNA/RNA/Protein Purification Kit E preps 68,89 Kit for purification of total DNA/RNA/Protein from the same biological sample. E preps 254,44 GeneMATRIX UNIVERSAL RNA Purification Kit E preps 53,33 Kit for purification of total RNA. E preps 196,67 GeneMATRIX UNIVERSAL RNA/miRNA Purification Kit E preps 58,89 Universal kit for isolation of total RNA and mirna from the tissue, plant and cell culture. E preps 218,89 Each kit buffers are also available in a separate sets. Price: 30 % of full kit price. EMULSION KITS: Cat.No. Package Micellula DNA Emulsion & Purification Kit E preps 83,33 Kit for creation of emulsions and subsequent DNA purification. For in vitro evolution, for in vitro E preps 233,33 compartmentalization (IVC) and for emulsion PCR (epcr). RESTRICTION ENDONUCLEASES: Enzyme Prototype Recognition sequence React. temp. o C Cat.No. Package AccI AccI GT / MKAC 37 E u 55,56 E u 222,22 AcvI PmaCI CAC / GTG 37 E u 40,00 E u 158,89 AluI AluI AG / CT 37 E u 36,67 E u 144,44 ApaI ApaI GGGCC / C 25 E u 50,00

9 E u 200,00 AvaI AvaI C / YCGRG 37 E u 40,00 E u 158,89 AvaII AvaII G / GWCC 37 E u 43,33 E u 173,33 BalI BalI TGG / CCA 37 E u 63,33 E u 253,33 BamHI BamHI G / GATCC 37 E u 40,00 E u 158,89 BanII HgiJII GRGCY / C 37 E u 45,56 E u 180,00 BglI BglI GCCNNNN / NGGC 37 E u 40,00 E u 158,89 BglII BglII A / GATCT 37 E u 40,00 E u 158,89 BsiHKCI AvaI C / YCGRG 65 E u 45,56 E u 180,00 BssHII BsePI G / CGCGC 50 E u 45,56 E u 180,00 BstXI BstXI CCANNNNN / NTGG 50 E u 45,56 E u 180,00 ClaI ClaI AT / CGAT 37 E u 51,11 E u 204,44 CviJI CviJI RG / CY 37 E u 125,56 E u 400,00 CviJI* Unique! CviJI* G / C except YG / CR 37 E u 165,56 E u 527,78 DdeI DdeI C / TNAG 37 E u 55,56 E u 222,22 DpnI DpnI GA / TC 37 E u 38,89 E u 150,00 DraI AhaI TTT / AAA 37 E u 36,67 E u 144,44 EcoRI EcoRI G / AATTC 37 E u 36,67 E u 144,44 E u 350,00 EcoRV EcoRV GAT / ATC 37 E u 36,67 E u 144,44 FokI FokI GGATG (9/13) 37 E u 40,00 E u 158,89 HaeIII HaeIII GG / CC 37 E u 36,67 E u 144,44 HhaI HhaI GCG / C 37 E u 55,56 E u 222,22 HincII HindII GTY / RAC 37 E u 40,00 E u 158,89 HindIII HindIII A / AGCTT 37 E u 36,67 E u 144,44 HinfI HinfI G / ANTC 37 E u 40,00 E u 158,89 HpaI HpaI GTT / AAC 37 E u 36,67 E u 144,44 HpaII HpaII C / CGG 37 E u 36,67 E u 144,44 KpnI KpnI GGTAC / C 37 E u 40,00 E u 158,89 MboI MboI / GATC 37 E u 40,00

10 E u 158,89 MboII MboII GAAGA (8/7) 37 E u 40,00 E u 158,89 MluI MluI A / CGCGT 37 E u 40,00 E u 158,89 MnlI MnlI CCTC (7/6) 37 E u 40,00 E u 158,89 MspI HpaII C / CGG 37 E u 40,00 E u 158,89 NarI NarI GG / CGCC 37 E u 40,00 E u 158,89 NcoI NcoI C / CATGG 37 E u 40,00 E u 158,89 NdeI NdeI CA / TATG 37 E u 27,78 E u 111,11 NheI NheI G / CTAGT 37 E u 27,78 E u 111,11 NotI NotI GC / GGCCGC 37 E u 50,00 E u 198,89 NruI NruI TCG / CGA 37 E u 40,00 E u 158,89 NsiI NsiI ATGCA / T 37 E u 55,56 E u 222,22 PinAI AgeI A / CCGGT 37 E u 61,11 E u 241,11 PstI PstI CTGCA / G 37 E u 45,56 E u 180,00 PvuI PvuI CGAT / CG 37 E u 36,67 E u 144,44 PvuII PvuII CAG / CTG 37 E u 40,00 E u 158,89 RsaI RsaI GT / AC 37 E u 40,00 E u 158,89 RsrII RsrII CG / GWCCG 37 E u 40,00 E u 158,89 SacI SacI GAGCT / C 37 E u 40,00 E u 158,89 SacII SacII CCGC / GG 37 E u 40,00 E u 158,89 SalI SalI G / TCGAC 37 E u 40,00 E u 158,89 Sau3AI MboI / GATC 37 E u temporary not E u available ScaI ScaI AGT / ACT 37 E u 40,00 E u 158,89 SfiI SfiI GGCCNNNN / NGGCC 50 E u 45,56 E u 180,00 SmaI SmaI CCC / GGG 25 E u 40,00 E u 158,89 SpeI SpeI A / CTAGT 37 E u 45,56 E u 180,00 SphI SphI GCATG / C 37 E u 40,00 E u 158,89 SspI SspI AAT / ATT 37 E u 45,56 E u 180,00 StuI StuI AGG / CCT 37 E u 40,00 E u 158,89 TaqI TaqI T / CGA 65 E u 40,00

11 E u 158,89 TaqII Unique! TaqII GACCGA (11/9) 65 E u 90,00 E u 360,00 TspDTI Unique! TspDTI ATGAA (11/9) 70 E u 90,00 E u 360,00 TspGWI Unique! TspGWI ACGGA (11/9) 70 E u 90,00 E u 360,00 Tth111I Tth111I GACN / NNGTC 65 E u 50,00 E u 200,00 XbaI XbaI T / CTAGA 37 E u 40,00 E u 158,89 XhoI XhoI C / TCGAG 37 E u 40,00 E u 158,89 LOS PRECIOS QUE SE INDICAN SON SIMPLEMENTE INFORMATIVOS Y PODRÁN VARIAR SIN PREVIO AVISO. CONFIRMAR VIGENCIA DE LOS MISMOS. IVA NO INCLUIDO