Plasmid DNA, Gel Extraction & PCR Products Purification Kits. Code Description Size. BS363 EZ-10 Spin Column PCR Products Purification Kit 50 preps

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1 Plasmid DNA, Gel Extraction & PCR Products Purification Kits Code Description Size BS363 EZ-10 Spin Column PCR Products Purification Kit BS364 EZ-10 Spin Column PCR Products Purification Kit 100 preps BS664 EZ-10 Spin Column PCR Products Purification Kit 2 BS Well Plate PCR Products Purification Kit 2x Plates BS Well Plate PCR Products Purification Kit 5X Plates BS367 EZ-10 Spin Column DNA Cleanup Mini-preps Kit BS368 EZ-10 Spin Column DNA Cleanup Mini-preps Kit 100 preps BS668 EZ-10 Spin Column DNA Cleanup Mini-preps Kit 2 BS Well Plate DNA Cleanup Mini-preps Kit 2x Plates BS Well Plate DNA Cleanup Mini-preps Kit 5X Plates BS353 EZ-10 Spin Column DNA Gel Extraction Kit BS354 EZ-10 Spin Column DNA Gel Extraction Kit 100 preps BS654 EZ-10 Spin Column DNA Gel Extraction Kit 2 BS413 EZ-10 Spin Column Plasmid DNA Mini-preps Kit BS414 EZ-10 Spin Column Plasmid DNA Mini-preps Kit 100 preps BS614 EZ-10 Spin Column Plasmid DNA Min-preps Kit 2 BS4139 EZ-10 Spin Column Plasmid DNA Mini-preps Kit, LOW COPY PLASMIDS BS4149 EZ-10 Spin Column Plasmid DNA Mini-preps Kit, LOW COPY PLASMIDS 100 preps BS6149 EZ-10 Spin Column Plasmid DNA Min-preps Kit, LOW COPY PLASMIDS 2 BS82014 Ultra-Fast Spin Column Plasmid DNA Min-Preps Kit BS4654 EZ-500 Spin Column Plasmid DNA Maxi-preps Kit 4 preps BS466 EZ-500 Spin Column Plasmid DNA Maxi-preps Kit 20 preps BS Well Plate Plasmid DNA Mini-Preps Kit 2X Plates BS Well Plate Plasmid DNA Mini-Preps Kit 5X Plates BS92028 Ultra-Fast 96-Well Plate Plasmid DNA Mini-Preps Kit 2X Plates BS92029 Ultra-Fast 96-Well Plate Plasmid DNA Mini-Preps Kit 5X Plates PT Well Plate Single Colony Plasmid DNA Mini-Preps Kit 2X Plates PT Well Plate Single Colony Plasmid DNA Mini-Preps Kit 5X Plates BS71918 EZ-10 Spin Column Endotoxin-Free Plasmid DNA Mini-Preps Kit 50 Preps SK1249 EZ-500 Spin Column Endotoxin-Free Plasmid DNA Maxi-Preps Kit 4 Maxi-Preps SK1250 EZ-500 Spin Column Endotoxin-Free Plasmid DNA Maxi-Preps Kit 20 Maxi-Preps BS467 EZ-10 Spin Column Yeast Plasmid DNA Mini-Preps Kit 10 Preps BS468 EZ-10 Spin Column Yeast Plasmid DNA Mini-Preps Kit 50 Preps BS469 EZ-10 Spin Column Yeast Plasmid DNA Mini-Preps Kit 250 Preps Genomic DNA Isolation Kits Code Description Size BS8202 EZ-DNA Reagents 100 Preps BS8413 One-Tube Bacteria Genomic DNA Isolation Kit 100 Preps BS8414 One-Tube Bacteria Genomic DNA Isolation Kit 500 Preps BS8225 Rapid Bacteria Genomic DNA Isolation Kit 50 Preps BS423 EZ-10 Spin Column Bacterial Genomic DNA Mini-Preps Kit 50 Preps BS624 EZ-10 Spin Column Bacterial Genomic DNA Mini-Preps Kit 250 Preps SK Well Plate Bacteria Genomic DNA Mini-Preps Kit 2X Plates 1

2 SK Well Plate Bacteria Genomic DNA Mini-Preps Kit 5X Plates PT92038 EZ-500 Spin Column Bacteria Genomic DNA Maxi-Preps Kit 4 Preps BS8227 Rapid Yeast Genomic DNA Extraction Kit 50 Preps BS8257 EZ-10 Spin Column Yeast Genomic DNA Mini-Preps Kit 50 Preps BS Well Plate Yeast Genomic DNA Mini-Preps Kit 2X Plates FT71415 Rapid Fungal Genomic DNA Isolation Kit 50 Preps FT82012 EZ-10 Spin Column Fungal Genomic DNA Mini-Preps Kit 50 Preps PT92037 EZ-500 Spin Column Fungal Genomic DNA Maxi-Preps Kit 4 Preps PT71816 One-tube Plan Genomic DNA Extraction Kit 100 Preps PT71817 One-tube Plan Genomic DNA Extraction Kit 250 Preps BS425 EZ-10 Spin Column Plant Genomic DNA Mini-Preps Kit 50 Preps BS626 EZ-10 Spin Column Plant Genomic DNA Mini-Preps Kit 250 Preps PT92035 EZ-500 Spin Column Plant Genomic DNA Maxi-Preps Kit 4 Preps PT92036 EZ-500 Spin Column Plant Genomic DNA Maxi-Preps Kit 20 Preps BS Well Plate Plant Genomic DNA Mini-Preps Kit 2X Plates ST82316 EZ-10 Spin Column Soil DNA Mini-Preps Kit 50 Preps BS8401 One-Tube Tissue DNA Extraction Kit 100 Preps BS8402 One-Tube Tissue DNA Extraction Kit 500 Preps AT4780 Rapid Animal Genomic DNA Extraction Kit 10 Preps AT4781 Rapid Animal Genomic DNA Extraction Kit 50 Preps AT4782 Rapid Animal Genomic DNA Extraction Kit 250 Preps BS427 EZ-10 Spin Column Animal Genomic DNA Mini-Preps Kit 50 Preps BS628 EZ-10 Spin Column Animal Genomic DNA Mini-Preps Kit 250 Preps PT92034 EZ-500 Spin Column Animal Genomic DNA Maxi-Preps Kit 4 Preps BS Well Plate Animal Genomic DNA Mini-Preps Kit 2X Plates BS Well Plate Animal Genomic DNA Mini-Preps Kit 5X Plates BS8403 One-Tube Clinical Sample DNA Extraction Kit 100 Preps BS8404 One-Tube Clinical Sample DNA Extraction Kit 500 Preps BT4782 Rapid Blood Genomic DNA Extraction Kit 50 Preps BS483 EZ-10 Spin Column Blood Genomic DNA MiniPreps Kit 50 Preps BS684 EZ-10 Spin Column Blood Genomic DNA MiniPreps Kit 250 Preps BT Well Plate Blood Genomic DNA Mini-Prep Kit 2 Plates BT Well Plate Blood Genomic DNA Mini-Prep Kit 5 Plates VT4785 One Tube Viral DNA Isolation Buffer 50 Preps VT4786 One Tube Viral DNA Isolation Buffer 100 Preps VT81812 EZ-10 Spin Column Viral DNA Mini-Preps Kit 50 Preps VT81813 EZ-10 Spin Column Viral DNA Mini-Preps Kit 250 Preps VT Well Plate Viral DNA Mini-Preps Kit 2X Plates T71612 One-Tube Swab DNA Extraction Kit 20 Preps T71613 One-Tube Swab DNA Extraction Kit 100 Preps T71614 One-Tube Swab DNA Extraction Kit 500 Preps 2

3 BS8407 One-Tube Hair DNA Extraction Kit 50 Preps BS88503 ONE-4-ALL Genomic DNA Miniprep Kit 50 Preps BS88504 ONE-4-ALL Genomic DNA Miniprep Kit 100 Preps BS88505 ONE-4-ALL Genomic DNA Miniprep Kit 250 Preps DNA/RNA/Protein Isolation Kit Code Description Size BS88003 All-In-One DNA/RNA/Protein Miniprep Kit 50 Preps BS88203 All-In-One DNA/RNA Miniprep Kit 50 Preps RNA Isolation Kit Code Description Size BS409A One Step RNA Reagent 25 ml BS410A One Step RNA Reagent 100 ml BS1361 EZ-10 Spin Column Total RNA Mini-preps Kit 50 Preps BS8625 Rapid Bacteria RNA Isolation Kit 50 Preps BS583 EZ-10 Spin Column Total RNA Mini-Preps Super Kit 20 Preps BS584 EZ-10 Spin Column Total RNA Mini-Preps Super Kit 100 Preps BS784 EZ-10 Spin Column Total RNA Mini-Preps Super Kit 250 Preps BS Well Plate Bacterial Total RNA Mini-Preps Super Kit 2X Plates BS Well Plate Bacterial Total RNA Mini-Preps Super Kit 5X Plates BS91315 EZ-10 Spin Column RNA Cleanup & Concentration Kit 50 Preps BRC Well Plate RNA Cleanup & Concentration Kit 2x Plates FT71416 Rapid Fungal RNA Extraction Kit 50 Preps BS91915 EZ-10 Spin Column Fungal RNA Mini-Preps Kit 50 Preps PT4191 Rapid Plant RNA Isolation Kit 50 Preps BS82314 EZ-10 Spin Column Plant RNA Mini-Preps Kit 50 Preps VT4184 Rapid Viral RNA Extraction Kit 50 Preps VT82112 EZ-10 Spin Column Viral Total RNA Extraction Kit 50 Preps AT4181 Rapid Animal Total RNA Extraction Kit 50 Preps AT4182 Rapid Animal Total RNA Extraction Kit 250 Preps BS82312 EZ-10 Spin Column Animal Total RNA Mini-Preps Kit 50 Preps BS82322 EZ-10 Spin Column Animal Total RNA Mini-Preps Kit 250 Preps BT4182 Rapid Blood RNA Isolation Kit 10 Preps BT4183 Rapid Blood RNA Isolation Kit 50 Preps BT4184 Rapid Blood RNA Isolation Kit 250 Preps BS82313 EZ-10 Spin Column Blood RNA Mini-Preps Kit 50 Preps MT91928 mrna Purification Kit from Total RNA 25 Preps BS88583 EZ-10 Total RNA Miniprep Kit 50 Preps BS88253 Rnase-Free DNAse Set 50 Preps BS88133 EZ-10 DNAway RNA Miniprep Kit 50 Preps 3

4 BS88136 EZ-10 DNAway RNA Miniprep Kit 250 Preps More Kits Code Description Size 9K Gel Extraction Kit (100 preps) 100 Preps 9K Gel Extraction Kit (200 preps) 200 Preps 9K PCR Purification Kit (100 preps) 100 Preps 9K PCR Purification Kit (200 preps) 100 Preps 9K Gel and PCR Extraction System (100 preps) 100 Preps 9K Gel and PCR Extraction System (200 preps) 200 Preps 9K Plasmid Miniprep Kit (100 preps) 100 Preps 9K Plasmid Miniprep Kit (200 preps) 200 Preps 9K Genomic DNA Extraction. Blood Genomic DNA Prep Kit (400Rx) 400 Rxn 9K Genomic DNA Extraction. Bacteria Genomic DNA Prep Kit (400Rx) 400 Rxn 9K Genomic DNA Extraction. Plant Genomic DNA Prep Kit (400Rx) 400 Rxn 9K Animal Tissue & Fungi Genomic DNA Extraction Prep Kit (400Rx) 400 Rxn 9K Yeast Genomic DNA Extraction Prep Kit (400Rx) 400 Rxn 9K Genomic DNA Extraction. Blood Genomic DNA Prep Kit (3300Rx) 400 Rxn 9K Genomic DNA Extraction. Bacteria Genomic DNA Prep Kit (3300Rx) 3300 Rxn 9K Genomic DNA Extraction. Plant Genomic DNA Prep Kit (3300Rx) 3300 Rxn 9K Animal Tissue & Fungi Genomic DNA Extraction Prep Kit (3300Rx) 3300 Rxn 9K Yeast Genomic DNA Extraction Prep Kit (3300Rx) 3300 Rxn 9K Plasmid DNA Maxiprep (10 preps) 10 Preps 9K Plasmid DNA Maxiprep (30 preps) 30 Preps 9K Plasmid DNA Midiprep (25 preps) 25 preps Columns & 96 Well Plates Code Description Size SD5005 EZ-10 Column & collection tube (blue tube, clear ring, clear collection) 100 SD5005- EMPTY EZ-10 Empty Column without filter, without collection tube & O-ring 1000 SD5008 EZ-10 RNA Column & collection tube(clear tube, clear ring, clear collection) 100 SD5003 EZ-200 Column & collection tube (set) 12 SD5004 EZ-500 Column & collection tube (set) 12 SD well filter plate (960ul each well) 12 SD5006- EMPTY 96 well filter plate (no filter) 12 SD well DNA plate with membrane (960ul each well) 12 SD well RNA plate with membrane (960ul each well) 12 SD well sample storage plate (200ul each well), 10/PK 1PK SD Well Vacuum System 1 SD5012 Medi preps or Maxi preps Column Plug 1 BR480-N 48 WELL 4ML U-SHAPED DEEP-WELL PLATES, 10/BAG 1PK BR581-96N 96 WELL 2ML DEEP PLATE, NATURAL,EDGE FILLED, 10PLATES/BAG 1PK 4

5 Bio Basic Inc. EZ-Kits Overview Bio Basic Inc. supplies a remarkable variety of molecular biology kits, including Plasmid Miniprep, Gel Extraction, PCR Product Purification, and a complete line of Genomic DNA and RNA isolation kits offered in both spin column and 96-well plate formats! Although the detailed protocol for each kit varies, the general three-step principle remains the same: (1) BIND, (2) WASH, (3) ELUTE. Bio Basic s EZ-Membrane allows for fast and efficient purification, regardless of sample type, thereby providing you with high yields of purified product with minimal handling and effort. Spin Column Format EZ-Protocol 96-Well Plate Format (1) BIND Starting material is bound to Bio Basic s EZ-Membrane in spin column or plate format. (2) WASH The bound material is washed to remove impurities, buffers, and other unwanted materials. (3) ELUTE The purified product (DNA/RNA/Protein) is eluted from the membrane and collected for downstream application! 5

6 Plasmid DNA, Gel Extraction & PCR Products Purification Kits EZ-10 Spin Column PCR Products Purification Kit This kit utilizes membrane technology which selectively adsorbs up to 20ug of PCR products or DNA fragments in the presence of a specialized binding buffer. Primers (<40-mer), nucleotides, enzymes, mineral oil and all other impurities are unable to bind and are eliminated from the column. PCR products or DNA fragments are eluted readily in elution buffer. PCR products and DNA fragments can be used readily in all downstream applications including digestion, cloning and DNA sequencing. The kit can also be used for concentrating DNA fragments as well as removal of impurities such as salts and proteins. 1. Fast. Using a rapid spin column format, the entire procedure take less than 10 minutes. 2. High Recovery. For fragments of 100 bp-10 kb, >85% of DNA will be recovered. 3. High Quality of DNA. Purified PCR products and DNA fragments can be used readily in all downstream applications including digestion, cloning and DNA sequencing. 4. Non-Toxic. No phenol/chloroform is required. 5. Direct manufacturer with most competitive price advantage. Transportation: At ambient temperature. BS Preps BS Preps BS Preps Buffer B3 16ml 32ml 80ml Wash Solution 12ml 24ml 60ml Elution Buffer 5ml 10ml 25ml EZ-10 Column ml Collection Tube Gel electrophoresis of PCR products purified using EZ-10 Spin Column PCR Products Purification Kit. 96-Well Plate PCR Products Purification Kit This kit provides a simple and efficient high throughput technique for the cleanup of PCR products and DNA fragments from gel extraction and enzymatic reaction solutions. PCR products or enzymatic reactions are mixed with a specialized binding buffer and pass through 96 columns of the plate. Impurities such as enzymes, salts, nucleotides and primers (< 40-mer) are washed away. Pure DNA is eluted with a small volume of low-salt elution buffer or water, and is ready for downstream applications including DNA sequencing and cloning. 1. Fast. Using a rapid spin column and 96-well high throughput format, the entire procedure take less than 30 minutes. 2. High Recovery. For fragments of 100 bp-10 kb, >85% of DNA will be recovered. 3. High Quality of DNA. Purified PCR products and DNA fragments can be used readily in all downstream applications including digestion, cloning and DNA sequencing. 4. Non-Toxic. No phenol/chloroform is required. 5. Direct manufacturer with most competitive price advantage. BS x 96 well Plates Buffer B3 60ml 2X75ml Wash Solution 2X35ml 4X48ml Elution Buffer 12ml 30ml EZ Well Plate 2 5 Deep Well Collection Plate Storage Plate 2 5 Sealing film 8 20 Complete 1 BS365 5 x 96 well Plates Reproducible yields of PCR products purified using the 96-Well Spin Column PCR Products Purification Kit. Lane 1: DNA marker. Lanes 2-5: Crude PCR products. Lanes 6 to 25: Purified PCR products. Recoveries and yields are approximately 80%. 6

7 EZ-10 Spin Column DNA Cleanup Mini-Preps Kit This kit provides a simple and efficient method for the purification of DNA fragments from various enzymatic reactions, including cdna synthesis, restriction digestions, and PCR, among others. It is also well-suited for desalting a solution of DNA, as well as the removal of residual organic solvents or unincorporated nucleotides or primers (<40-mer) from reaction mixtures. The kit utilizes spin column technology which selectively adsorbs up to 20ug of DNA in the presence of specialized binding buffers. All impurities that do not bind are eliminated from the column. DNA fragments can be eluted readily with elution buffer. Purified DNA fragments and PCR products can be used for most downstream applications including sequencing and cloning. 1. Fast. Using a rapid spin column format, the entire procedure takes less than 10 minutes. 2. High Recovery. For fragments of 100 bp-10 kb, >85% of DNA will be recovered. 3. High Quality of DNA. Purified PCR products and DNA fragments can be used readily in all downstream applications, including digestion, cloning and sequencing. 4. Non-Toxic. No phenol/chloroform is required. 5. Direct manufacturer with most competitive price advantage. BS Preps BS Preps BS Preps Clean Up Solution 20ml 40ml 4X25ml Wash Solution 20ml 2X20ml 2X40ml Elution Buffer 5ml 10ml 25ml EZ-10 Column ml Collection Tube Gel electrophoresis of PCR products purified using EZ-10 Spin Column DNA Cleanup Mini-preps Kit. 96-Well Plate DNA Cleanup Mini-Preps Kit This kit provides a simple and efficient high throughput method for the cleanup of PCR products and other DNA fragments from gel extraction solutions and enzymatic reactions. The kit utilizes a simple bind-wash-elute procedure. PCR products or other enzymatic reactions are mixed with a specialized binding buffer and pass through 96 columns of the plate. Whereas DNA binds to the column, impurities such as enzymes, salts, nucleotides and primers (< 40-mer) are washed away. Pure DNA can be eluted in a small volume of the low-salt elution buffer or water, ready for use in all downstream applications including DNA sequencing and cloning. Phenol/chloroform extraction and ethanol precipitation are not required. Recovering yield is typically 60-80%. Up to 20ug of purified DNA fragments in each well can be obtained. It is suitable to recover 100 bp-40 kb DNA fragments. 1. Fast. Using a rapid spin column and 96-well high throughput format, the entire procedure takes less than 30 minutes. 2. High recovery. For fragments of 100 bp-10 kb, >85% of DNA will be recovered. 3. High quality of DNA. Purified PCR products and DNA fragments can be used readily in all downstream applications including digestion, cloning and DNA sequencing. 4. Non-Toxic. No phenol/chloroform is required. 5. Direct manufacturer with most competitive price advantage. Transportation: At ambient temperature. BS x 96 well Plates Clean Up Solution 60ml 2X75ml Wash Solution 2X35ml 4X48ml Elution Buffer 12ml 30ml EZ Well Plate 2 5 Deep Well Plate Well Storage Plate 2 5 Sealing film BS369 5 x 96 well Plates Reproducible yields of high quality PCR products purified using the EZ well Plate DNA Cleanup Mini-Prep Kit. 7

8 EZ-10 Spin Column DNA Gel Extraction Kit This kit provides a simple and rapid method for the purification of DNA fragments from agarose gel. It is also well-suited for desalting, as well as the removal of residual organic solvents or unincorporated nucleotides or primers (<40-mer) from reaction mixtures containing DNA. The kit utilizes a spin column technology which selectively adsorbs up to 20ug of DNA fragments in the presence of specialized binding buffers. Nucleotides, enzymes, mineral oil and other impurities do not bind to the column and are eliminated. DNA fragments can be eluted readily in elution buffer and used for DNA sequencing, restriction digestion, and other downstream applications. Recovering yield ranges from 60 to 80%. No phenol/chloroform extraction or ethanol precipitation is required. 1. Fast. Using a rapid spin column format, the entire procedure takes less than 15 minutes. 2. High Recovery. Yield ranges from 60 to 80%. 3. Direct manufacturer with most competitive price advantage. Transportation: At ambient temperature. BS Preps BS Preps BS Preps Binding Buffer II 50ml 2X50ml 5X50ml Wash Solution 20ml 2X20ml 2x40ml Elution Buffer 5ml 10ml 25ml EZ-10 Column ml Collection tube DNA fragments purified from an agarose gel using the Gel Extraction Kit. EZ-10 Spin Column Plasmid Mini-Preps Kit This kit provides a simple and efficient method for the purification of plasmid DNA from bacterial culture. Plasmid DNA is selectively adsorbed by the EZ-10 column, while impurities such as proteins, salts and nucleotides are washed away. In order to maximize the recovery yield of plasmid DNA, a color indicator VisualLyse - is added to the buffer, which prevents insufficient or over-lysis during the lysis and neutralization step. Note: This kit is designed for the preparation of up to 20ug of plasmid DNA. If larger amounts are required, plasmid Maxi preps are recommended. For low copy plasmid, BS4139, BS4149 and BS6149 are recommended. Solutions are doubled in low copy plasmid kits. 1. Fast. Using a rapid spin column format, the entire procedure takes only 12 minutes. 2. High Purity of DNA. OD260/OD280 ratio of purified DNA is , and can be used directly in most downstream applications such as sequencing, PCR, transformation, restriction digestion and ligation. 3. Non-toxic. No phenol/chloroform extraction or ethanol precipitation is required. 4. Highest yield is guaranteed by using VisulLyse reagent. 5. Direct manufacturer with most competitive price advantage. Transportation: At ambient temperature.. BS Preps BS Preps BS Preps Solution I 6ml 12ml 30ml Solution II* 12ml 24ml 60ml Solution III 25ml 50ml 125ml Wash Solution** 20ml 2X20ml 2X40ml Elution Buffer 5ml 10ml 25ml RNase A (10mg/ml) 120ul 240ul 600ul VisualLyse 60ul 120ul 300ul EZ-10 Column ml Collection Tube *Solution II may form a precipitate upon storage. If necessary, dissolve the precipitate by warming at 37 0 C. ** Before use for the first time, add four volumes of 96%-100% ethanol to Wash Solution (v/v). Gel electrophoresis of plasmid DNA purified using the EZ-10 Plasmid DNA Mini-preps Kit. 8

9 Ultra-Fast Spin Column Plasmid DNA Mini-Preps Kit This kit facilitates single-step plasmid DNA purification from bacterial cultures. Using a one buffer system as opposed to the traditional three, the kit releases plasmid DNA rapidly from your sample. Although the yield of the recovered plasmid DNA is slightly lower than that from regular kits, it is much faster and simpler to use. Bacterial cultures are lysed and the lysates are directly loaded on to the spin column. Plasmid DNA is selectively bound onto the EZ-10 column and other impurities are washed away. Pure plasmid DNA is eluted in low-salt buffer or water, and can be readily used for many downstream applications. This kit is suitable for the preparation of up to 8ug of pure plasmid DNA. 1. Fast and Simple: One step lysis of cell cultures. The entire procedure takes less than 10 minutes. 2. High Purity of DNA: OD260/OD280 of plasmid DNA purified using this kit is >1.8. Purified plasmid DNA can be used for many downstream applications, including DNA sequencing, cloning, PCR, transformation and restriction digestions. 3. Non-toxic: phenol/chloroform extraction and ethanol precipitation are not required. 4. Direct manufacturer with most competitive price advantage. Transportation: At ambient room temperature. Lysis Buffer UF Buffer DW1 Universal Wash Solution Elution Buffer BS Preps 50ml 24ml 12ml 5ml EZ-10 Column ml Collection Tube 50 Plasmid DNA purified using the Ultra-Fast EZ-10 Spin Column Plasmid DNA Mini-preps Kit. EZ-500 Spin Column Plasmid DNA Maxi-Preps Kit This kit provides a simple and efficient method for purifying large amounts of plasmid DNA. Up to 500ug of plasmid DNA is selectively adsorbed on the 25ml EZ-500 column. Impurities, including proteins, salts and nucleotides, are eliminated from the column and washed away. Plasmid DNA can be eluted in 3ml of Elution buffer. No phenol/chloroform or ethanol precipitations are required. DNA purified using the kit can be used in many downstream applications including transformation, DNA sequencing, PCR, and Southern-blotting. In order to obtain a maximal recovery yield, a color indicator - VisualLyse - is added to the buffer which prevents insufficient or over-lysis during the lysis and neutralization step. Specification: Sampling Yield Handling Time 100~250 ml of bacteria culture for high copy plasmids up to 500 mg ~2 hours 200~400 ml of bacteria culture for low copy plasmids for high-copy plasmid Transportation: At ambient temperature. BS4654 BS466 4 Preps 20 Preps MAXI 1 Resuspension Solution 55 ml 2X110 ml MAXI 2 Cell Lysis Solution 55 ml 2X110 ml MAXI 3 Neutralization Solution 55 ml 2X110 ml MAXI 4 Equilibration Solution 54 ml 2X135 ml MAXI 5 Washing Solution 4X55 ml 4X275 ml MAXI 6 Elution Solution 54 ml 2X135 ml RNase A (50 mg / ml) 88 ml 440 ml MAXI Column 4 20 Collection Tube Solutions provided in this kit contain irritants, wear gloves and lab coat when handling. 2. Briefly spin RNase A tube to remove drops from the inside of the lid. Transfer tube content into MAXI 1 Resuspension Solution bottle. Add 250µL of MAXI 1 Resuspension Solution into RNase A tube, rinse tube inside and transfer back into MAXI 1 Resuspension Solution bottle. Store at 4 C. 3. Check MAXI 2 Cell Lysis Solution before use. Warm MAXI 2 Cell Lysis Solution at 37 C if any precipitation formed. Prevent vigorous shaking of the MAXI 2 Cell Lysis Solution. 4. To avoid acidification of MAXI 2 Cell Lysis Solution from CO 2 in the air, close the bottle immediately after use. 9

10 96-Well Plate Plasmid DNA Mini-Preps Kit This kit provides a rapid, high-throughput method for 96-well plasmid DNA purification. Plasmid DNA is selectively adsorbed on silica membranes in the 96-well plate. Proteins, salts, nucleotides and other contaminants are subsequently eliminated from the column. Purified plasmid DNA can then be eluted in a small volume of elution buffer. In order to obtain maximal yield, a color indicator VisualLyse - is added to the buffer which prevents insufficient or over-lysis during the lysis and neutralization step. Purified DNA can be used directly in a variety of applications including sequencing, PCR and Southern-blotting. Note: This kit is used for the preparation of a maximum of 20ug of pure plasmid DNA per well. 1. Fast. Using the rapid spin column and high-throughput format, the entire procedure takes only 30 minutes. 2. High DNA Yield. Up to 20μg DNA in each well can be obtained. 3. High Purity of DNA. OD260/OD280 ratio of purified DNA is typically Non-toxic. No phenol/chloroform extraction or ethanol precipitation is required. 5. Direct manufacturer with most competitive price advantage. Transportation: At ambient temperature. BS plates BS415 5 plates Solution I 25ml 60ml Solution II* 50ml 120ml Solution III 90ml 210ml Wash Solution** 2X35ml 4X48ml Elution Buffer 15ml 30ml RNase A (10mg/ml) 0.5ml 1.2ml VisualLyse 240ul 600ul EZ Well Plate 2 5 Deep Well Collection Plate Storage Plate 2 5 Reproducible yields of plasmid DNA purified using the 96- Well Plate Plasmid DNA Mini-preps Kit. Sealing film *Solution II may form a precipitate upon storage. If necessary, dissolve the precipitate by warming at 37 C. ** Before use for the first time, add four volumes of 96%-100% ethanol to Wash Solution. Ultra-Fast 96-Well Plate Plasmid DNA Mini-Preps Kit This kit is designed for one-step plasmid DNA purification from bacterial culture using a rapid and high throughput spin column format. Using a one buffer solution and replacing the traditional three, the kit releases plasmid DNA rapidly from bacterial culture. Although the yield of the recovered plasmid DNA is slightly lower than that from regular 96-Well Plate Kits, it is simpler and faster. Bacterial cultures are lysed and the lysates are directly loaded to each column of the 96-Well plate. Plasmid DNA is selectively bound onto the 96-well plate and other impurities are washed away. Pure plasmid DNA is eluted in Elution buffer or water, and can be readily used for most downstream applications. This kit is used for preparation of up to 6-8ug of pure plasmid DNA. 1. Fast & Simple. Using one step lysis spin column and 96-well high throughput format, the entire procedure takes only 20 minutes. 2. High Purity of DNA. OD260/OD280 of plasmid DNA purified by this kit is >1.8. Purified plasmid DNA can be used for many downstream applications such as DNA sequencing, cloning, PCR, transformation and restriction digestions. 3. Non-toxic. No phenol/chloroform extraction and ethanol precipitation are required. Transportation: At ambient temperature. BS plates BS plates Lysis-Buffer-UF 200ml 2X250ml Buffer DW1 100ml 2X120ml Universal Wash Solution II 48ml 2X48ml Elution Buffer 20ml 50ml EZ Well Plate 2 5 Deep Well Collection Plate Storage Plate 2 5 Sealing film

11 96-Well Plate Single Colony Plasmid DNA Mini-Preps Kit This kit provides a simple, efficient and high throughput method for plasmid DNA purification. Plasmid DNA and DNA fragments are selectively adsorbed onto the EZ-10 columns of the 96-well plate. Other impurities such as proteins, salts and nucleotides are washed away. Plasmid DNA can then be eluted in a small volume of elution buffer. Purified plasmid DNA can be used for many downstream applications, including PCR, DNA sequencing, enzyme digestion and transformation. Note: the diameter of each colony should be >2 mm. 1. Fast. Using a rapid spin column and 96-well high throughput format, the entire procedure takes 20 minutes. 2. Direct amplification from colonies, or glycerol stock solutions, no overnight culture is required. 3. Suitable for fast and large number screening. 4. Compatible with all downstream applications including PCR, restriction analysis and DNA sequencing. 5. Economic. Transportation: at ambient room temperature. PT plates PT plates Lysis-Buffer-UF 200ml 2X250ml Buffer DW1 100ml 2X120ml Universal Wash Solution II 48ml 2X48ml Elution Buffer 20ml 50ml EZ Well Plate 2 5 Deep Well Collection Plate Storage Plate 2 5 Sealing film puc plasmid DNA purified using the 96-well single colony plasmid DNA MiniPrep Kit. EZ-10 Spin Column Endotoxin-Free Plasmid DNA Mini-Preps Kit This kit provides a simple and efficient method for high quality endotoxin-free plasmid DNA purification from bacterial culture. Greater than 90% of the endotoxin can be removed using our Endotoxin-Be-Gone reagent. If large scale preparation is needed, EZ-500 Column can be used (SK1249 & SK1250). Purified endotoxin-free plasmid DNA is suitable for transfection and other downstream applications. 1. Fast and convenient. Endotoxin is removed at the bacteria stage and the remaining steps are regular plasmid DNA extraction steps. 2. Efficient. A single treatment removes 90% of the endotoxin. Repeat procedure for greater efficiency. Purified plasmid DNA can be used for many downstream applications including transfection. 3. Economic. Solution I Solution II Solution III Wash Solution Elution Buffer Liquid Endotoxin Eliminator EZ-10 Column 50 RNase A Solution (10mg/ml) 2.0ml Collection Tube 50 BS Preps 6ml 12ml 25ml 20ml 5ml 5ml 120μl 11

12 EZ-500 Spin Column Endotoxin-Free Plasmid DNA Maxi-Preps Kit This kit provides a large-scale, simple and efficient method for high quality endotoxin-free plasmid DNA purification from bacterial culture. Up to 500ug of endotoxin-free plasmid DNA may be obtained per maxi prep. Greater than 90% of the endotoxin can be removed using bacterial endotoxin erasol at bacteria stage. Purified endotoxin-free plasmid DNA can be used for transfection and other downstream applications. 1. Fast. Using a maxi-prep spin-column format, the entire procedure takes only 40 minutes. 2. High Quality of DNA. >90-99% of endotoxin can be removed. 3. Economic. Store: at room temperature. The kit is stable for 1 year. Buffer Solution I with RNase A should be kept at 2-8 C, valid for 6 months. SK Maxi-Preps SK Maxi-Preps Solution I 100ml 2 X 240 ml Solution II 200ml 4 x 240ml Solution III 2X150ml 6 x 250ml Wash Solution 10ml 48ml Elution Buffer 8ml 40ml Liquid Endotoxin Eliminator 8ml 40ml RNase A Solution (10mg/ml) 2ml 10ml EZ-500 Column ml Collection Tube EZ-10 Spin Column Yeast Plasmid DNA Mini-Preps Kit The kit provides a simple and efficient Spin Column approach for Mini plasmid DNA purification from yeast. Plasmid DNA of lysed yeast cells is selectively bound onto the column. Other impurities such as proteins and salts do not bind to the column and are eliminated in the flow through. Purified plasmid DNA can be used for PCR, transformation, restriction digestion and many other downstream applications.1ug of plasmid DNA can be purified from 5ml yeast cells. 1. Fast. Using a rapid spin-column format, the entire procedure takes approximately 2 hours. 2. High Quality of DNA. OD260/OD280 of purified DNA is generally > Compatible with most downstream applications such as PCR, restriction digestion and hybridization. 4. Non-Toxic. Phenol/chloroform extractions or ethanol precipitation is not required. 5. Direct manufacturer with most competitive price advantage. Transportation: at ambient temperature BS Preps BS Preps BS Preps Buffer P1 3ml 15ml 75ml Buffer P2 3ml 15ml 75ml Buffer P3 4ml 20ml 100ml Buffer DW1 5ml 25ml 125ml Universal Wash Buffer 3ml 12ml 60ml Elution Buffer 1ml 5ml 25ml RNase A Solution (10mg/ml) 25ul 120ul 600ul Snailase 60mg 300mg 1500mg Snailase Storage Buffer 0.5ml 2.5ml 12.5ml Snailase Reaction Buffer 7ml 35ml 175ml EZ-10 Spin Column ml Collection Tube

13 Genomic DNA Purification & Extraction Kits EZ-DNA Reagent EZ-DNA Reagent is a ready-to-use reagent for the isolation of genomic DNA from various samples, including animal tissue, cultured cells, and bacteria. The method is based on the use of an optimized guanidine-detergent solution, which lysis the tissue quickly and selective precipitates DNA from a cell lysate. During the isolation step, tissue or cells are lysed in EZ-DNA Reagent and the genomic DNA is precipitated from the lysate with ethanol. Following an ethanol wash, purified DNA is dissolved in water, TE buffer or 8mM NaOH. The protocol allows for fast isolation of genomic DNA from a large number of samples of small or large volume. The whole procedure takes about minutes with DNA recovery of 70-90%. There is no need for overnight digestion, phenol extraction. Isolated DNA can be used directly for most applications such as Southern analysis, molecular cloning and PCR. 1. Fast. The entire procedure takes only minutes. 2. Simple. One-Tube procedure, no phenol/chloroform extraction is required. 3. Versatile. Suitable for wide range of samples including animal tissue, cultured cells, and bacteria. 4. Easy to scale up. 5. Economic. Transportation: t ambient temperature. BS preps EZ-DNA Reagent 100ml DNA extraction from various species using EZ-DNA Reagent. M. DNA marker. Lane 1-3: Genomic DNA from Bacteria. Lane 4-6: Genomic DNA from Rat. Lane 7-9: Genomic DNA from Yeast. One-Tube Bacteria Genomic DNA Isolation Kit The kit is designed for rapid small-scale extraction of high quality genomic DNA from a variety of Gram-negative and Gram-positive bacteria. Purified DNA can be used for most downstream applications, including PCR, restriction digestion, and hybridization. 1. Fast. Te entire procedure takes less than 10 minutes. 2. Simple. No phenol/chloroform extraction or ethanol precipitation is required. 3. Economic. Transportation: At ambient temperature. BS Preps BS Preps Lysis-Buffer-B 10ml 50ml Universal Buffer NST 10ml 50ml 1 DNA isolated from various bacteria. M: DNA marker. Lane 1-3: Escherichia coli (Gram-Negative). Lane 4-6: Staphyloccocus aureus (Gram-Positive). Rapid Bacteria Genomic DNA Isolation Kit The kit is designed for rapid small-scale extraction of high quality genomic DNA from a variety of Gram-negative or Gram-positive bacteria. Purified DNA can be used for many downstream applications such as PCR, restriction digestion, and hybridization. 1. Fast. It takes less than 30 minutes. 2. Simple. 3. Economic. Transportation: Room temperature Universal Digestion Buffer Buffer PB BS Preps 25ml 12ml 13

14 TE Buffer 10ml EZ-10 Spin Column Bacterial Genomic DNA Mini-Preps Kit This kit provides a simple and convenient silica membrane-based technique to isolate high quality DNA from both Gram-negative and Grampositive bacteria. Total hands-on preparation time is less than 30 minutes. Bacteria samples are lysed in an optimized buffer. Released DNA binds specifically to the column while contaminants are removed in the flowthrough. PCR inhibitors such as divalent cations and proteins are completely removed in two efficient wash steps. Pure DNA is then eluted in either water or a low-salt buffer provided with the kit. The kit is also suitable for isolation of bacterial genomic DNA from colonies on a dish. Purified genomic DNA is kb in length. Purified DNA is suitable for downstream applications such as restriction endonuclease digestions, PCR, and other applications. 1. Fast. Using a rapid spin-column format, the entire procedure takes approx 20 minutes. 2. High yield. 5-20ug of bacterial genomic DNA can be obtained from 1ml ( cells) of overnight culture. 3. High quality of DNA. OD260/280 ratio of purified DNA is generally > Non-Toxic. No phenol extraction or ethanol precipitation is required. 5. Economic Transportation: At ambient temperature. BS Preps BS Preps Digestion Buffer 20ml 100ml Wash Solution 12ml 2X30ml Elution Buffer 5ml 25ml Proteinase K 2mg 10mg EZ-10 Spin Column ml Collection Tube DNA isolated from various bacteria. M: DNA Marker Lane 1-3: Escherichia coli (Gram Negative) Lane 4-6: Staphyloccocus aureus (Gram Positive) 96-Well Plate Bacterial Genomic DNA Mini-Preps Kit The kit provides a simple and convenient high throughput approach to isolate high quality DNA from both Gram-negative and Gram-positive bacteria. DNA in a cell lysate is selectively adsorbed on to the EZ-10 column while impurities such as proteins, salts and nucleotides are washed away. No phenol/chloroform extraction or ethanol precipitation is required. Purified genomic DNA can be up to 50 kb in length. Genomic DNA purified can be used for PCR, and other downstream applications. 1. Suitable for Gram-positive and Gram-negative bacteria. 2. Fast. Using a high throughput 96-well plate format. 3. High purity of DNA. A260/280 ratio of purified DNA is generally > Compatible with downstream applications. Transportation: At ambient temperature. SK Plates SK Plates Digestion Buffer 160ml 2X200ml TE (ph=8.0) 60ml 150ml Wash Solution 50ml 3X48ml Elution Buffer 12ml 30ml Proteinase K 8mg 20mg 96-Well Plate 2 5 Deep Well Collection Plate Well Storage Plate 2 5 Sealing film E. coli genomic DNA purified using the 96-well plate mini-preps kit. Shown on 0.8% agarose with DNA ladders. 14

15 EZ-500 Spin Column Bacteria Genomic DNA Maxi-Preps Kit This kit provides a simple and convenient technique for large-scale isolation of high quality DNA from both Gram-negative and Gram-positive bacteria. Up to 500ug of DNA is selectively bound to EZ-500 spin column. Other impurities present in the lysate, including proteins and salts, do not bind on the column and are eliminated in the flow through. No phenol extraction or ethanol precipitation is required. The kit is also suitable for the isolation of bacterial genomic DNA from colonies grown on a dish. Purified genomic DNA can be up to 50 kb in length. Purified DNA is suitable for downstream applications such as restriction endonuclease digestions, PCR, and other applications. For isolation of DNA from Gram positive bacteria, bacteria should be pre-treated with a buffer containing lysozyme. 1. Suitable for both Gram-positive and Gram-negative bacteria. 2. High purity of DNA. Purified DNA has an A260/280 ratio of between 1.6 and Compatible with many downstream applications. 4. Direct manufacturer with most competitive price advantage. PT Preps Universal Digestion Buffer 40ml Universal Buffer BD 50ml Universal PW Solution 18ml Universal Wash Solution 7.5ml CE Buffer ( ph 9.0) 15ml Proteinase K (10mg/ml) 5ml Maxi Columns 4 50ml Collection Tube M E.coli genomic DNA purified using the EZ-500 Spin Column Bacteria DNA Maxi-Preps Kit. Shown on a 0.8% agarose gel. M: DNA Marker 1-7: genomic DNA from E.coli Rapid Yeast Genomic DNA Extraction Kit This kit is designed for rapid, small-scale extraction of high quality genomic DNA from yeast. Yeast cell walls are digested by Snailase (or lyticase, zymolyase) and the membrane is lysed in the presence of proprietary buffers. DNA is then precipitated and washed with alcohol. Purified DNA can be used for many downstream applications, including PCR, restriction digestion, and hybridization. 1. Fast. The entire procedure takes less than 40 minutes. 2. Easy to use. 3. Economic. BS Preps Universal Digestion Buffer Buffer PY TE Buffer Snailase Reaction Buffer Snailase Storage Buffer Snailase 25ml 12ml 10ml 75ml 5ml 600mg Genomic DNA isolated from Saccharomyces cerevisiae Lane 1-3: Genomic DNA from Saccharomyces cerevisiae. 15

16 EZ-10 Spin Column Yeast Genomic DNA Mini-Preps Kit The kit provides a simple and convenient method for the isolation of high quality yeast DNA. DNA present in cell lysate is selectively bound to the spin column while other impurities such as proteins and salts do not bind and are eliminated in the flow through. Purified genomic DNA is kb in length, and is suitable for many downstream applications, including restriction endonuclease digestion, PCR, and cloning. 1. Fast. Using a rapid spin-column format, the entire procedure takes approx 30 minutes. 2. High Quality of DNA: OD260/OD280 of purified DNA is generally Non-Toxic. No phenol extraction or ethanol precipitation is required. 4. Economic. Transportation: At ambient temperature including Proteinase K and Snailase. BS Preps Universal Digestion Buffer 12ml Universal Buffer PY 6ml Universal Buffer BD 12ml Universal PW Solution 18ml Universal Wash Solution 7.5ml CE Buffer ( ph 9.0) 15ml Proteinase K 1.2ml Snailase Reaction Buffer 75ml Snailase Storage Buffer 5ml Snailase 600mg EZ-10 Spin Column ml Collection Tube 50 Genomic DNA isolated from Saccharomycetaceae 96-Well Plate Yeast Genomic DNA Mini-Preps Kit This kit provides a simple and convenient, high-throughput approach to isolate high quality DNA from yeast. DNA present in cell lysate is selectively adsorbed onto the EZ-10 column, while impurities such as proteins, salts and nucleotides are washed away. Purified genomic DNA can be up to 50 kb in length, and can be used for a wide array of downstream applications, including PCR and restriction endonuclease digestions. 1. Fast. using the rapid spin-column and high throughput format, the entire procedure takes only 2 hours. 2. High quality of DNA. OD260/280 of purified DNA is generally Non-toxic. No phenol extraction or ethanol precipitation is required. 4. Economic. Transportation: at ambient temperature (including Proteinase K and Snailase). BS Plates Universal Buffer Digestion Universal Buffer PY Universal Buffer BD Universal PW Solution Universal Wash Solution CE Buffer (ph 9.0) Proteinase K Snailase Reaction Buffer Snailase Storage Buffer Snailase 96-Well EZ Plate 2 96-Well Collection Plate 6 96-Well Storage Plate 2 Sealing film 8 48ml 24ml 48ml 72ml 30ml 15ml 4.8ml 300ml 20ml 2.4 g Saccharomyces cerevisiae genomic DNA purified using the 96-well plate Yeast Genomic DNA Mini-Prep Extraction Kit. 16

17 Rapid Fungal Genomic DNA Isolation Kit This kit provides a simple and efficient method for the isolation of total DNA (genomic and mitochondrial) from fungal species. The size of genomic DNA purified by this procedure is around kb. Purified DNA can be readily used in a number of downstream applications including RT-PCR, SNP analysis, and restriction digestion. 1. Fast. the entire procedure takes less than 40 minutes. 2. Easy. 3. Economic. Universal Digestion Buffer Universal Buffer PF TE Buffer FT ml 12ml 10ml DNA isolated from various fungal species M: Marker Lane 1-2: Penicillium spp. Lane 3-4: Pleurotus ostreatus. EZ-10 Spin Column Fungal Genomic DNA Mini-Preps Kit This kit provides a simple and convenient method for the isolation of high quality DNA from various fungal species. DNA present in a cell lysate is selectively bound to the spin column, while other impurities such as proteins, salts, and nucelotides do not bind and are eliminated in the flow through. No phenol extraction or ethanol precipitation is required. Purified genomic DNA is kb in length, and is suitable for downstream applications, including restriction digestions, cloning, and PCR. 1. Fast and Simple. Using a spin-column format, the entire procedure takes only 30 minutes. 2. High quality of Fungal DNA. 3. Economic. FT82012 Universal Digestion Buffer Universal Buffer PF Universal Buffer BD Universal PW Solution Universal Wash Solution TE Buffer (ph 8.0) Proteinase K 12ml 6ml 12ml 18ml 7.5ml 10ml EZ-10 Spin Column 50 2-ml Collection Tube ml Price $ Genomic DNA isolated from various fungi Lane 1-3: Penicillium spp. genomic DNA Lane 4-6: Pleurotus ostreatus. DNA EZ-500 Spin Column Fungal Genomic DNA Maxi-Preps Kit This kit provides a simple and efficient method for the isolation of genomic DNA from a variety of fungal species. DNA present in a cell lysate is selectively adsorbed in the column while other impurities are eliminated. Average DNA yield is ~10-100ug per preparation. Average size of the genomic DNA purified by this procedure is around kb. No phenol/chloroform extraction, ethanol precipitation, or liquid nitrogen homogenization is required in this protocol. Purified fungal genomic DNA can be used for PCR, SNP, RAPD, RFLP, AFLP, Southern blotting, transformation and other applications. 1. Fast. 2. High Quality of DNA. OD260/280 ratio of purified fungal genomic DNA is generally > Suitable for various filamentous fungal species including S. cerevisiae, S. pombe, Pichia pastoris, Candida albicans, Aspergillus niger, Collectotrichum musae, and Fusarium oxysporum. PT preps Universal Digestion Buffer 50ml Universal Buffer PF 25ml Universal Buffer BD 50ml Universal PW Solution 18ml Universal Wash Solution 7.5ml TE Buffer (ph 8.0) 10ml Proteinase K 5ml EZ-500 Spin Columns 4 Purified fungal genomic DNA run on a 0.7% agarose gel.

18 50-ml Collection Tube 4 Rapid Plant Genomic DNA Isolation Kit This kit is designed for the rapid isolation of genomic DNA from plant leaves and seeds. Samples may be fresh, frozen, or dried. The whole procedure takes approximately 15 minutes and the kit is suitable for screening and high throughput extraction. No phenol/chloroform extraction or ethanol precipitation is required. Purified Genomic DNA can be used for RT-PCR, cloning and other downstream applications. 1. Fast. The entire procedure takes less than 30 minutes. 2. Easy. 3. Direct manufacturer with most competitive price advantage. PT preps PT preps Buffer PCL 50ml 250ml Buffer PP 24ml 120ml TE Buffer 20ml 100ml 1 High molecular genomic DNA isolated from various plant species using the Rapid Plant DNA Kit. Lane 1-2: Genomic DNA from Nicotiana tabacum Lane 3-4: Genomic DNA from Arabidopsis thaliana Lane 5-6: Genomic DNA from Oryza sativa M: DNA marker. EZ-10 Spin Column Plant DNA Mini-Preps Kit This kit provides a simple and efficient method for the isolation of plant genomic DNA. Samples may be fresh, frozen, or dried. DNA in plant tissues & cells is selectively adsorbed on the spin column, while other impurities such as proteins, salts, and lipids do not bind to the column and are washed away. Average size of genomic DNA purified by this procedure is ~30-50 kb. Purified genomic DNA can be used directly in PCR, Southern Blotting, RAPD, RFLP, and many more. 1. Fast. Using a rapid spin-column format, the entire procedure takes less than 30 minutes. 2. High Purity of DNA. OD260/280 of purified DNA is generally > Compatible with many downstream applications such as PCR, restriction digestions, real-time PCR, multiplex PCR, RAPD, RFLP, AFLP, Southern Blotting and microsatellite analysis. 4. Suitable for a wide variety of plant species and tissue types including some recalcitrant specimens. BS425 BS626 2 RNase A (10mg/ml) 150ul 750ul PCL Solution 15ml 75ml PP Solution 2ml 10ml PB Solution 20ml 100ml Wash Solution 12ml 2X30ml Elution Buffer 5ml 25ml EZ-10 Spin Column ml Collection Tube Genomic DNA isolated from various plant species using the EZ-10 Plant DNA mini-prep kit. Lane 1-2: Nicotiana tabacum Lane 3-4: Arabidopsis thaliana Lane 5-6: Oryza sativa Lane 7-8: Platycladus orientalis Lane 9-10: Wisteria sinensis M: DNA marker 18

19 EZ-500 Spin Column Plant Genomic DNA Maxi-Preps Kit PT preps/ PT preps The kit is designed for the isolation of large quantities of genomic DNA from a wide variety of plant species. Samples may be fresh, frozen, or dried. DNA in plant tissues and cells are selectively adsorbed on the EZ-500 maxi-prep spin column, while other impurities such as proteins, salts, and lipids do not bind to the column and are washed away. Up to 500ug of plant DNA can be obtained per preparation. Average size of DNA purified using this procedure is ~30-50 kb. Purified genomic DNA can be used directly for PCR, Southern Blotting, RAPD, and more. 1. High Purity of DNA. OD260/280 of purified DNA is generally > Compatible with many downstream applications, including such as PCR, RAPD, RFLP, AFLP, Southern Blotting and microsatellite analysis. 3. Suitable for a wide variety of plant species and tissue types including some very recalcitrant specimens. Transportation: At ambient temperature. E.coli genomic DNA purified using the EZ-500 Spin Column Plant DNA Maxi-Preps Kit. Shown on a 0.8% agarose gel. M: DNA Marker 1-7: genomic DNA from E.coli M 96-Well Plate Plant Genomic DNA Mini-Preps Kit This kit provides a rapid and convenient high-throughput technique for the preparation of high quality genomic DNA from various plant species. DNA in a plant lysate is selectively adsorbed on each column of the plate while other impurities such as proteins, salts, and lipids are eliminated from the column. Each column can adsorb up to 20ug of DNA. No phenol/chloroform extraction or ethanol precipitation is required. Purified plant genomic DNA is kb, and is suitable for an array of downstream applications including PCR, Southern blotting, microsatellite analysis, AFLP, RFLP, and RAPD. 1. Fast. Using a spin column format, the entire procedure takes less than 2 hours. 2. High purity of DNA. OD260/280 of purified DNA is generally > Compatible with many downstream applications. 4. Suitable for a wide variety of plant species and tissue types including some very recalcitrant specimens. Universal Buffer PCB Universal Buffer BD Universal PW Solution Universal Wash Solution TE Buffer (ph 8.0) 96-Well EZ Plate 2 96-Well Collection Plate 6 96-Well Storage Plate 2 Sealing film 8 BS Plates 130ml 50ml 72ml 30ml 40ml Plant genomic DNA purified using the 96-well Plate DNA Mini-Prep Kit. 19

20 EZ-10 Spin Column Soil DNA Mini-Preps Kit This kit provides PCR-inhibitor free DNA through a convenient and rapid method for the detection of microorganisms from soil, sand and fecal samples. These samples are considered challenging as they contain rich humic acid that can interfere with the PCR reaction. The kit removes all traces of humic acid using an optimized buffer during the spin column procedure. Briefly, the soil sample is lysed using lysis buffer (Buffer SCL). DNA is then bound to an EZ-10 column in the presence of chaotropic salt. Under these conditions only DNA will bind to the membrane while most of the contaminating RNA, proteins, and cell debris are removed in the flow through. Purified inhibitor-free DNA is then eluted into ul of the provided TE buffer or water. Total genomic DNA can be isolated and purified from various microorganisms found in soil, including bacteria, fungi and algae. Purified humic acid-free DNA is of the highest quality and is fully compatible with PCR applications or other downstream applications. The molecular size of the purified DNA is around kb. Average DNA yields are 5-20ug per gram of soil sample. 1. Fast. Using a rapid spin column format, the entire procedure takes 30 minutes. 2. Versatile. All types of soil samples can be processed with this kit, including sand and fecal samples. 3. High Quality of DNA. OD260/OD280 of purified DNA is generally The purified DNA is almost free from all inhibitors including humic acid. 4. Economical ST82316 SCL Solution SP Solution SB Solution Wash Solution Elution Buffer 25ml 25ml 40ml 12ml 5ml Spin Column 50 2ml Collection Tube 50 DNA purified from soil samples. One-Tube Tissue DNA Extraction Kit This kit facilitates rapid isolation of genomic DNA from a wide array of sample types, including animal tissue, mouse tail and ear clips, human hair or saliva, and fish and insect tissue. There is no need for phenol extraction, overnight digestion, DNA precipitation or column purification; the lysate can be used in PCR directly. One-tube extraction kit minimizes the cross-contamination between samples. The procedure takes less than 15 minutes, and the kit is suitable for high throughput PCR screening of large numbers of samples. For other applications, Rapid Animal DNA Isolation Kit and EZ-10 Spin Column Animal DNA Mini-preps Kit (codes: BS427 AT4781; AT4780; BS428) are recommended. Samples recommended: cm mouse tail; or mm mouse ear punch; or 2-5mg piece of tissue; or 1-10 hairs with roots; or 20µl saliva; or 2-3 mm 2 piece of zebrafish fin. 1. Fast. The entire procedure takes less than 15 minutes. 2. High Quality of DNA. OD260/OD280 of purified DNA is generally Versatile. Compatible with tissues from mice, fish and insects, human hair, saliva, swab, blood clot and so on. 4. Easy to scale up. 5. Economic. BS Preps BS Preps Lysis-Buffer-T 20ml 100ml Proteinase K 2ml 10ml Universal Buffer NST 20ml 100ml 1 PCR amplification using DNA isolated by One-Tube Tissue DNA Extraction Kit as template. Lane 1-3: template DNA from Rat muscle Lane 4-6: template DNA from Rat liver 20

21 Rapid Animal Genomic DNA Extraction Kit This kit is designed for rapid small-scale extraction of high quality genomic DNA from a variety of fresh or frozen animal cells and tissues. Purified DNA can be used for many downstream applications such as PCR restriction digestion, hybridization and other applications. 1. Rapid and simple. The entire procedure takes only 20 minutes. 2. High Quality of DNA. OD260/280 ratio of purified DNA is generally > Non-Toxic. 4. Easy to scale up. 5. Economic. AT Preps AT Preps AT Preps Universal Buffer Digestion 5ml 24ml 120ml Buffer PA 3ml 12ml 60ml TE Buffer 2ml 10ml 50ml 1 1 Fig. DNA isolated from various animal tissues Lane 1-2: Rat muscle DNA Lane 3-4: mouse liver DNA Lane 5-6: fish DNA EZ-10 Spin Column Animal Genomic DNA Mini-Preps Kit The kit is designed for rapid small scale extraction of genomic DNA from various animal tissues (fresh or frozen). DNA from cell lysate is selectively adsorbed on the spin column, while other impurities such as proteins, salts, and lipids are eliminated. No phenol extraction or ethanol precipitation is required. Purified DNA can be used for many downstream applications including PCR, restriction digestion, and hybridization. 1. Fast. Using a rapid spin-column format, the entire procedure takes only 20 minutes. 2. High Quality of DNA. OD260/OD280 ratio of purified DNA is generally > Compatible with many downstream applications. 4. Economic. BS Preps BS Preps ACL Solution 20ml 100ml PBS Solution 75ml 2X200ml AB Solution 20ml 100ml CW2 Solution 9ml 45ml Wash Solution 12ml 2X30ml Elution Buffer 5ml 25ml Proteinase K 20mg 100mg EZ-10 Spin Column ml Collection Tube Gel electrophoresis of an animal genomic DNA sample purified using the EZ-10 Spin Column Animal DNA Minipreps Kit. Lane 1 is DNA marker. 21

22 EZ-500 Spin Column Animal Genomic DNA Maxi-Preps Kit This kit provides an efficient method for the purification of large quantities of animal genomic DNA. DNA in tissue lysates is selectively adsorbed on the EZ-500 spin column, while other impurities are unable to bind and eliminated in the flowthrough. No phenol extraction or ethanol precipitation is required. Purified genomic DNA can be used directly in a wide array of downstream applications. 1. Fast. Uses a rapid spin-column format, the entire procedure takes 20 minutes only. 2. High Quality of DNA. OD260/OD280 ratio of purified DNA is generally > Suitable for large scale extraction of DNA from fresh or frozen animal tissues or cells. 4. Economic. Universal Buffer ACL Universal Buffer CL CW1 Solution CW2 Solution CE Buffer Proteinase K EZ-500 Column 4 50-ml Collection Tube 4 PT preps 50ml 60ml 13ml 9ml 20ml 6ml Gel electrophoresis of an animal genomic DNA sample purified by using EZ-500 Spin Column Animal Genomic DNA Maxi-Preps Kit. Lane 1 is DNA marker. 96-Well Plate Animal Genomic DNA Mini-Preps Kit This kit provides a rapid and convenient high-throughput technique for the preparation of high quality genomic DNA from animal tissues and cells. DNA in a cell lysate is selectively adsorbed in the column while other impurities such as proteins, salts, and lipids are eliminated from the column. Each column can retain up to 20ug of DNA. No phenol/chloroform extraction or ethanol precipitation is required. Purified genomic DNA can be used for PCR and many other downstream applications. 1. Fast. Using a high-throughput spin column format, the entire procedure takes only 15 minutes. 2. High Quality of DNA. OD260/OD280 ratio of purified DNA is generally > Suitable for extraction of DNA from fresh or frozen animal tissues or cells. BS Plates BS437 5 Plates ACL Solution 80ml 200ml PBS Solution 80ml 200ml AB Solution 80ml 200ml CW2 Solution 80ml 200ml Wash Solution 2X35ml 4X48ml Elution Buffer 20ml 50ml Proteinase K 80mg 200mg EZ Well Plate 2 5 Deep Collection Plate 4 10 Genomic DNA from various animal tissues purified using the 96-well plate mini-preps kit. Shown on a 0.8% agarose gel. 96 Storage Plate 2 5 Sealing film

23 One-Tube Clinical Sample DNA Extraction Kit This kit is designed for rapid isolation of genomic DNA from clinical samples including blood, blood clots, saliva, hair, swabs, and cell shedding. A simple and rapid lysis method is utilized, and there is no need for phenol extraction, overnight digestion, DNA precipitation or column purification. DNA can be used in PCR directly. The procedure takes less than 15 minutes to complete, and the kit is suitable for high throughput PCR screening of large-scale samples. Samples recommended: 5-20µl blood; or 20µl saliva; or 2-5mg blood clot; or Swab. 1. Fast. The entire procedure takes less than 15 minutes. 2. Suitable for screening and high-throughput automation. 3. Easy to scale up. 4. Economical. BS Preps BS Preps Lysis Buffer-L 10ml 50ml 1 PCR amplification of DNA isolated using the One-Tube Clinical Sample DNA Extraction Kit. Lane 1-4: template DNA from human anti-coagulated blood. (+) Positive Control (-) Negative Control Rapid Blood Genomic DNA Extraction Kit This kit facilitates the simple and rapid isolation of high quality genomic DNA from fresh or frozen anti-coagulated blood. Whole blood of mammalian animals consists of non-nucleated cells (e.g. red blood cells, platelets) and nucleated cells (e.g. white blood cells). The former is lysed and removed by a Red Blood Cell Lysis buffer (Buffer TBP), while the latter (containing genomic DNA) is collected and lysed by Buffer Digestion in the presence of Proteinase K. DNA is released to the solution and can be isolated by ethanol precipitation. Up to 5ml of blood can be used in each prep. Average DNA yields are 20-60μg per ml of whole blood. Purified DNA can be used directly in a wide range of downstream applications. 1. Fast. The entire procedure takes 30 minutes. 2. Simple. 3. High Quality of DNA. OD260/OD280 ratio of purified DNA is generally > Easy to scale up. 5. Economical. Transportation: At ambient temperature. BT Preps Buffer TBP 40ml Universal Buffer Digestion 10ml Buffer PR 4ml Proteinase K 1.2ml TE Buffer 50ml Genomic DNA purified from whole animal blood using the Rapid Blood Genomic DNA Extraction Kit. 1: Chicken Blood. 2: Rabbit Blood. 3: Pig Blood. M: lambda DNA. Shown on 0.8% agarose 23

24 EZ-10 Spin Column Blood Genomic DNA Mini-Preps Kit This kit provides a simple, rapid and efficient technique for isolation of high molecular weight genomic DNA from fresh, frozen and anticoagulated whole blood. DNA from cell lysate is selectively adsorbed on the column while other impurities, including proteins and lipids, are unable to bind and are washed away. Purified genomic DNA can be used in a number of downstream applications, including PCR, Southern blotting and SNP genotyping. 1. High quality of DNA. OD260/OD280 ratio of purified DNA is generally > Fast. Using a rapid spin-column format, the entire procedure takes 20 minutes. 3. Non-Toxic. 4. Easy. No red blood cell lysis step required. 5. Economical. BS Preps BS684 2 TBP Buffer 120ml 60ml TBM Buffer 25ml 65ml TE (ph 8.0) 15ml 45ml Wash Solution 12ml 2X30ml Elution Buffer 5ml 25ml Proteinase K 2mg 10 mg EZ-10 Spin Column ml Collection Tube Genomic DNA preparations from a single blood sample of human whole blood using the Kit. 96-Well Plate Blood Genomic DNA Mini-Prep Kit This kit provides a simple, rapid and high-throughput method for the isolation of high quality genomic DNA from blood. DNA in a lysate is selectively adsorbed on each column in the plate while other impurities such as proteins, salts, and lipids are washed away. Purified genomic DNA can be used for PCR, Southern blot analysis and other applications. Average yield is about 20-60ug per ml of mammalian whole blood sample. 1. High Quality of DNA. OD260/OD280 ratio of purified DNA is generally > Non-Toxic. No phenol extraction and no ethanol precipitation are required. 3. Easy. No red blood cell lysis step require 4. Economical. BT plates BT plates PBS Solution 40ml 100ml Universal Buffer CL 48ml 120ml CW1 Solution (concentrate) 52ml 130ml CW2 Solution (concentrate) 36ml 90ml CE Buffer 40ml 120ml Proteinase K 4.8ml 12ml EZ Well Plate 2 5 Deep Well Collection Plate Storage Plate 2 5 Sealing film 8 20 Genomic DNA purified from human blood using the 96-well plate Blood Genomic DNA Mini-preps Kit. 24

25 One-Tube Viral DNA Isolation Buffer The One Tube Viral DNA Isolation Buffer is used for isolation of viral DNA from a broad range of cell-free clinical samples including serum, urine and plasma. The one-tube procedure is simple and rapid; no transfer between tubes is necessary, which helps in minimizing crosscontamination. Purified viral DNA can be used for PCR, Real Time PCR, and many other downstream applications. 1. High yield. The recovery yield is generally >85%. 2. Simple. 3. Compatible with many downstream applications including PCR and Real Time PCR. 4. Easy to scale up. 5. Economic. VT4785 VT preps Lysis Buffer-V 30ml 60ml 1 Viral DNA was extracted from 0.2ml blood plasma with HBV titer of 1000 copies/ml and dissolved in 200ul water. A 5uL aliquot was used in realtime PCR. Red and green line represent our product; the Blue line represents a product from a competitor. EZ-10 Spin Column Viral DNA Mini-Preps Kit This kit provides a simple, rapid and highly reproducible method for the isolation of viral DNA from a broad range of cell-free clinical samples, including serum, urine and plasma. Viral DNA in lysate is selectively adsorbed in the spin column while impurities are unable to bind and are removed in the flow through. PCR inhibitors, proteins and salts are completely removed during wash steps. Purified nucleic acids are then eluted in either water or TE buffer. The procedure is simple and fast. Purified viral DNA can be used for PCR, Real Time PCR and other clinical research applications. 1. Fast and Simple. Using a rapid spin-column format the entire procedure takes only 20 minutes. 2. Sensitive virus copies in 1ml of sample can be detected by PCR. 3. Non-Toxic. No phenol/chloroform or ethanol precipitation is required. 4. Versatile. Purified viral DNA can be used for PCR, Real Time PCR and other clinical applications. Suitable for broad range cell-free clinical samples including serum, urine and plasma. VT81812 VT Lysis-Buffer-V 30ml 150ml Universal Wash Solution 15ml 75ml TE Buffer 10ml 50ml Spin Column ml Collection Tube

26 96-Well Plate Viral DNA Mini-Preps Kit This kit provides a fast, simple and high-throughput technique for the isolation of viral DNA from a broad range of cell-free clinical samples, including serum, urine and plasma. Viral DNA in lysates is selectively adsorbed in each spin column of the 96-well plate, while other impurities are unable to bind and are washed away. The procedure is simple and rapid. Purified viral DNA can be used for PCR, Real Time PCR and other clinical applications. 1. Fast and Simple. Using a rapid spin-column & 96-well high throughput format, the entire procedure takes only 30 minutes. 2. Sensitive virus copies in 1ml of sample in each well can be detected by PCR. 3. Non-Toxic. No phenol/ chloroform and ethanol are required. 4. Compatible. Purified viral DNA can be used for PCR, Real Time PCR and other clinical applications. 5. Versatile. Suitable for broad range cell-free clinical samples including serum, urine and plasma. 6. Economical. VT plates Lysis-Buffer-V 120ml Universal Wash Buffer 60ml Universal Elution Buffer 20ml EZ Well Plate 2 Deep Well Collection Plate 4 96 Storage Plate 2 Sealing film 8 One-Tube Swab DNA Extraction Kit This kit is designed for one-tube isolation of PCR-ready genomic DNA from a variety of swab materials, including buccal swab and virginal swab. There is no need for phenol extraction, overnight digestion, DNA precipitation or column purification, and the lysate can be use as a PCR template directly. The one-tube manipulation minimizes the cross-contamination between specimens. The kit is also suitable for DNA extraction from many other clinical samples such as blood, dried blood and sperm. Purified swab DNA can be used for PCR. 1. Fast and Simple. 2. Suitable for extraction of DNA from a wide range of clinical samples. 3. Non-Toxic. 4. Economical. T Preps T Preps T Preps Lysis-Buffer-S 4ml 20ml 100ml Proteinase K 0.4ml 2ml 10ml Universal Buffer NST 4ml 20ml 100ml One-Tube Hair DNA Extraction Kit This kit provides a simple and rapid method for the isolation of genomic DNA from hair and other forensic samples, including saliva, nails and dried blood. The kit includes Proteinase K and other reagents to break up most tissue samples as well as to separate proteins and other components from the DNA. No phenol extraction, overnight digestion, DNA precipitation or column purification. Lysate can be used directly as a PCR template. The one-tube manipulation minimizes cross-contamination between specimens. 1. Fast and Simple. 2. Suitable for extraction of DNA from a wide range of clinical samples, including buccal swab, virginal swab, blood, dried blood and sperm. 3. Non-Toxic. 4. Economic. BS Preps Lysis-Buffer-H 1.6ml Proteinase K 60ul 26

27 ONE-4-ALL Genomic DNA Mini-Preps Kit The kit is designed for rapid small scale extraction of genomic DNA from various sources of starting materials. DNA from cell lysate is selectively absorbed on the Spin Column, and other impurities such as proteins, salts are eliminated. No phenol extraction, no ethanol precipitations are required. Purified DNA can be used for many downstream applications such as PCR, restriction digestion, hybridization and other applications. Fast. Using a rapid spin-column format, the entire procedure takes 20 minutes. High Quality of DNA. OD260/OD280 ratio of purified DNA is generally >1.8. Flexible and versatile. Isolation of genomic DNA from various sources. Transportation: At ambient temperature. BS Preps BS Preps Buffer ACL 10ml 50ml Buffer CL 12ml 60ml CW1 Solution 13ml 65ml CW2 Solution 9ml 45ml CE Buffer 15ml 75ml Proteinase K 1.25ml 5X1.25ml EZ-10 Spin Column ml Collection Tube Gel electrophoresis of an animal genomic DNA sample purified by using ONE-4-ALL Genomic DNA Minipreps Kit. Lane 1 is DNA marker. All-In-One DNA/RNA Mini-Preps Kit All-In-One DNA/RNA kit is designed for simultaneous extraction of total RNA and genomic DNA from a single biological sample. DNA and RNA are isolated without splitting the sample prior to extraction. DNA and RNA can be isolated from cultured eukaryotic cells, animal and plant tissues. 1. Efficient. Genomic DNA and RNA can be simultaneously isolated in 1hr. 2. Simple. No phenol/chloroform extraction is required. 3. Versatile. Suitable for wide range of samples including animal tissue, cultured cells, and bacteria. 4. Easy to scale up. 5. High yield and reproducibility. Transportation: At ambient temperature. BS88203 Buffer Lysis-DR 45 ml CW1 Solution 13 ml CW2 Solution 9 ml CE Buffer 10 ml GT Solution 18 ml NT Solution 6 ml RNase Free Water 5 ml EZ-10 DNA Column 50 RZ-10 RNA Column 50 27

28 DNA/RNA/Protein Extraction Kits All-In-One DNA/RNA/Protein Mini-Preps Kit All-In-One DNA/RNA/Protein kit is designed for simultaneous extraction of total RNA, genomic DNA and protein from a single biological sample. DNA, RNA and protein are isolated without splitting the sample prior to extraction. DNA, RNA and protein can be isolated from cultured eukaryotic cells, animal and plant tissues. Efficient. Genomic DNA, RNA and proteins can be simultaneously isolated in 1hr. Simple. No phenol/chloroform extraction is required. Versatile. Suitable for wide range of samples including animal tissue, cultured cells, and bacteria. Easy to scale up. High yield and reproducibility. BS88003 Buffer Lysis-DRP 45 ml CW1 Solution 13 ml CW2 Solution 9 ml CE Buffer 10 ml GT Solution 18 ml NT Solution 6 ml RNase Free Water 5 ml PP Solution 35 ml PD Solution 10 ml EZ-10 DNA Column 50 RZ-10 RNA Column 50 RNA Extraction & Purification Kits EZ-RNA Reagents EZ-RNA Reagents are used for the isolation of total RNA from cells and tissues. During sample homogenization or lysis, the reagents maintain the integrity of the RNA. Addition of chloroform followed by centrifugation separates the solution into an aqueous phase and an organic phase. RNA is then recovered by precipitation with ethanol or isopropyl alcohol. EZ-RNA Reagents perform well with both small and large quantities of tissue and cells, and are suitable for human, animal, plant or bacterial samples. RNA species of a wide MW range are isolated. The isolated RNA typically has an OD260/OD280 ratio of when diluted into RNase-free water at ph 8.0, and can be used in most downstream applications. 1. High recovery yield. 2. Simple and rapid. The entire procedure takes only 10 minutes. 3. High Purity of RNA. 4. Compatible with many downstream applications, including RT-PCR, Northern Blot, and cdna synthesis. 5. Versatile. Suitable for RNA extraction from most sources including bacteria, fungi, animals and some plants. 6. Easy to scale up. 7. Economic. 25ml and 100ml of EZ-RNA Reagents are sufficient for 50 and 200 mini-preps. Total RNA isolation by EZ-RNA Reagents. M: DNA marker. Lane 1-2: Nicotiana tabacum total RNA. Lane 3-4: Fish total RNA. BS409A 50 Preps BS410A 100 Preps One Step RNA Reagent 25ml 100ml 1 28

29 EZ-10 Spin Column Total RNA Mini-preps Kit This kit is designed for the purification of total RNA from a wide range of sample types, including bacteria, fungi, animals and plants. This kit simplifies total RNA isolation by combining the stringency of guanidine isothiocyanate lysis with the speed and purity of silica-membrane based purification. Samples are first lysed and then homogenized. Ethanol is added to the lysate to provide optimal binding conditions. The lysate is then loaded onto the EZ column. RNA binds to the silica membrane, while proteins and other contaminants are removed in the flow-through. Purified RNA is then eluted in RNase-free Water, and is ideal for use in most downstream applications, including Northern blotting, RT-PCR, Quantitative PCR, Poly A+ RNA selection and Array analysis 1. Fast. Using a rapid spin column format, the whole procedure takes less than 20 minutes. 2. High Quality of RNA. Purified RNA has an OD260/OD280 ratio of Versatile. Suitable for purification of total RNA from various samples such as bacteria, fungi, animals and some plants. 4. Economic. BS Preps Buffer RLT 25ml Buffer RW 30ml Universal RPE Solution 12ml RNase-free water 5ml Spin Columns 50 Collection Tubes 50 Rapid Bacteria RNA Isolation Kit This kit is designed for the preparation of high quality total RNA from bacterial cells. 20μg total RNA can be purified from 5 x 10 7 bacterial cells using this kit. Purified RNA is ready for most downstream applications including RT-PCR, Northern Blotting, Poly (A) purification, nuclease protection and in vitro translation 1. Fast. Using fast lysis buffer, the whole procedure takes less than 40 minutes. 2. High Quality of RNA. Purified RNA has an OD260/OD280 ratio of Easy to scale up. 4. Economical. Total bacterial RNA isolated using the Rapid RNA isolation Kit. BS Preps Buffer Rlysis-B 50ml RNase-free Water 5ml EZ-10 Spin Column Bacterial Total RNA Mini-Preps Kit This kit provides a simple spin column technique for preparation of high-purity intact total RNA. The reagent contains guanidine isothiocyanate and β-mercaptoethanol that can inactivate ribonucleases present in cell extracts. RNA is selectively absorbed on spin column and other impurities are washed away. Total RNA is eluted in he presence of RNase-Free Water. 5-15μg total RNA can be purified from 25mg animal tissue using this kit. Purified RNA is ready for most downstream applications such as RT-PCR, Northern Blotting, Poly(A)+ selection and in vitro translation. 1. Fast. Using rapid spin column format and fast lysis buffer the whole procedure takes less than 30 minutes. 2. High Quality of RNA. Purified RNA has an OD260/OD280 ratio of Economical. BS preps BS preps RLT Solution 14ml 70ml 175ml RW Solution 12ml 60ml 150ml RPE Solution 5ml 22ml 230ml BS784 2 RNase-free Water 1ml 5ml 12.5ml EZ-10 Column ml Collection Tube Fig. 1. Total RNA purified from E. coli using the EZ10 Column Bacteria Total RNA Mini-Prep Kit. M: Marker;

30 96-Well Plate Bacterial Total RNA Mini-Preps Super Kit This kit provides a simple and rapid method for the extraction of high quality total RNA from a wide range of biological samples, including Gramnegative and Gram-positive cultures. RNA in a bacterial lysate is selectively bound on each column of the 96-well plate while other impurities such as proteins and salts are washed away. The entire procedure takes approximately 15 minutes. No ethanol precipitation is required. Purified bacterial total RNA can be used for many downstream applications such as RT-PCR, Northern Blot, and cdna synthesis. The kit is also suitable for RNA extraction from animal, fungi and some of plants samples. The kit may not be suitable for RNA extraction from the plants which contain high levels of secondary metabolites, polyphenols and polysaccharides. Plant RNA Isolation Kit and EZ-10 Spin Column Plant RNA Isolation Kit are recommended (Codes PT4191 and BS82314 ). 1. Fast. Using a rapid spin and 96-well high throughput format, the entire procedure takes less than 15 minutes only. 2. High Purity of RNA. Purified RNA has an OD260/OD280 ratio of Suitable for RNA extraction from both of Gram negative and positive bacteria. 4. Economic. BS plates BS585 5 plates Buffer Rlysis-BG 80ml 200ml Universal GT Solution 72ml 180ml Universal NT Solution 24ml 60ml RNase-free Water 10ml 25ml EZ Well Plate 2 5 Deep Collection Plate well Storage Plate 1 5 Sealing Film Reproducible yields of total RNA from a single sample using EZ well Spin Column Plate RNA Minipreps Kit. The bands at two ends are RNA markers EZ-10 Spin Column RNA Cleanup & Concentration Kit. This kit is designed for the rapid purification and concentration of total RNA from in vitro transcription products or from various other materials. More than 80% of the RNA in a given sample can be recovered, and is ready for most downstream applications, such as RT-PCR, Northern Blotting and in vitro translation. 1. Fast. Using a rapid spin-column format, the entire procedure takes only 5 minutes! 2. High Purity of RNA. Purified RNA can directly used for RT-PCR, electrophoresis and labelling reaction, Northern Blot and cdna synthesis. 3. High recovery yield. 4. Economic. BS91315 Buffer RLT 50ml Universal RPE Solution 12ml RNase-free Water 5ml EZ-10 Spin Column 50 2-ml Collection Tube 50 30

31 96-Well Plate RNA Cleanup & Concentration Kit. This kit is designed for the rapid purification and concentration of total RNA from in vitro transcription products or various other materials. More than 80% of the RNA can typically be recovered, and is ready for most downstream applications including RT-PCR, Northern Blotting and in vitro translation. 1. Fast. Using a rapid spin-column and 96-well high throughput format, the entire procedure takes 10 minutes. 2. High Yield: Up to 80% of the RNA will be recovered. 3. High Efficiency: Complete removal of contaminants 4. Economic. Buffer RLT Universal RPE Solution RNase-free Water 96-Well Plate 2 2 ml Deep Well Collection Plate 4 96-Well Storage Plate 2 Caps for Storage Plate 24 Sealing film 8 BRC Plates 200ml 48ml 20ml Rapid Fungal RNA Extraction Kit This kit is designed for the preparation of high quality total RNA from a wide range of fungal species. Total RNA can be purified from fresh or frozen filamentous fungi samples using this kit. Purified RNA is ready for most downstream applications including RT-PCR, Northern Blotting, Poly (A) purification, nuclease protection and in vitro translation. 1. Simple and rapid. The entire procedure takes only 40 minutes. 2. High quality of RNA. OD260/OD280 ratio of purified RNA is generally > Easy to scale up. 4. Economic. Transportation: at ambient temperature FT71416 Buffer Rlysis-F 50ml RNase-free Water 5ml Total RNA purified from Pichia pastoris using the Rapid Fungal RNA Isolation Kit. 31

32 EZ-10 Spin Column Fungal RNA Mini-Preps Kit This kit is designed for the preparation of high quality total RNA from a wide range of fungal species. Fungal samples are first lysed and homogenized using Buffer FS. RNA in the homogenate is then selectively adsorbed on to the spin column. Finally, the total RNA is eluted from the membrane by the addition of RNase-Free Water. 3-5μg total RNA can be purified from 30mg filamentous fungi using this kit. Purified RNA is ready for most downstream applications such as RT-PCR, Northern Blotting, Poly A+ purification, nuclease protection and in vitro translation. 1. Fast. Using a rapid spin-column format, the entire procedure takes approximately 20 minutes. 2. High quality of RNA. OD260/280 of purified RNA is generally > Intact RNA. RNA is purified with intergrity. 4. Economic. Transportation: at ambient temperature Buffer Rlysis-FG Universal GT Solution Universal NT Solution RNase-free Water BS ml 18ml 6ml 5ml EZ-10 Spin Column 50 2ml Collection Tube 50 Total RNA isolated from filamentous fungi using the Fungal RNA Mini-Preps Kit. Lane 1-3: Fungal total RNA. Rapid Plant RNA Isolation Kit This kit is designed for the preparation of high quality total RNA from a wide variety of plant species and tissue types. Plant tissue is first lysed and homogenized using Buffer Rlysis-P. All contaminants, including polysaccharides and lipids, are removed by centrifugation. Purified RNA is ready for most downstream applications such as RT-PCR, Northern Blotting, Poly (A) purification, nuclease protection and in vitro translation 1. Fast. The whole procedure can be completed in 40 minutes 2. High Quality of RNA. The OD260/OD280 of purified RNA is generally > Versatile. Suitable for isolation of total RNA from a wide range of specimens such as Arabidopsis thaliana, tobacco, camphor and other samples. 4. Easy to scale up. 5. Economical. Transportation : at ambient temperature PT4191 Buffer Rlysis-P 35ml Buffer PK 4ml RNase-free Water 5ml Total RNA from a plant leaf purified using the Plant RNA Extraction Kit 32

33 EZ-10 Spin Column Plant RNA Mini-Preps Kit Plant polysaccharides and polyphenols have a tendency to form insoluble compounds with RNA, making the isolation of plant RNA a sometimes-difficult task. Our Total Plant RNA Purification Kit resolves this problem and can help you obtain the RNA samples you require for your research. RNA Purification using this kit is easy to operate, and the purified RNA sample is ready for most downstream applications such as RT-PCR, Northern Blotting, Poly A+ purification, nuclease protection and in vitro translation. 1. Fast. Using a rapid spin-column format the entire procedure takes only 30 minutes. 2. Versatile. Suitable for the isolation of RNA from a wide range of specimens such as Arabidopsis thaliana, tobacco, camphor and other samples. 3. High Quality of RNA. The OD260/OD280 of purified RNA is generally > Economic. Transportation: at ambient temperature BS82314 Buffer Rlysis-PG 25ml Universal GT Solution 18ml Universal NT Solution 6ml RNase-free Water 5ml EZ-10 Column 50 2-ml Collection Tube 50 Total RNA isolation from various plant species using the EZ10 Spin Column Plant RNA Mini-Prep Kit. Lane 1-2: Arabidopsis thaliana. Lane 3-4: Nicotiana tabacum. Lane 5-6: Spinacia oleracea. Lane 7-8: Oryza sativa. Lane 9-10: Cupressaceae. Lane 11-12: Cinnamomum camphora. Rapid Viral RNA Extraction Kit This kit is designed for the preparation of high quality viral RNA from cell-free liquid specimens, including blood serum, urine, body fluids. This method is fast and easy to perform, and no mechanical disruption or column purification is required. Purified RNA is ready for most downstream applications including RT-PCR, Northern Blotting, Poly A+ purification, nuclease protection and in vitro translation. 1. High recovery yield. >90% of viral RNA can be recovered. 2. Simple and rapid procedure. Entire procedure takes less than 30 minutes and can be completed in one tube. 3. High Quality of viral RNA: Complete removal of contaminants. Isolation of total RNA for use in a large range of applications 4. Economic. Transportation: at ambient temperature VT4184 Buffer VG 30ml RNase-free Water 5ml RNA isolated by Rapid Viral RNA Isolation Kit in Real-time PCR 33

34 EZ-10 Spin Column Viral Total RNA Extraction Kit This kit simplifies the isolation of viral RNA from cell-free body fluids with a fast spin-column format. Viral RNA binds specifically to the silica membrane while contaminants are removed in the flow-through. PCR inhibitors such as divalent cations and proteins are completely removed in two washing steps, leaving pure viral RNA to be eluted in RNase-free Water. Purified RNA is ready to use in RT-PCR, Northern blotting or other downstream applications. 1. Fast. Using a rapid spin column format, the entire procedure takes about 20 minutes. 2. High Yield. The recovery yield of viral RNA is generally >85% 3. Versatile. Suitable for purification of viral RNA from a wide range of specimens, including serum, plasma, cell culture media, and milk. 4. Non-toxic. No Phenol/chloroform extraction. 5. Economic. Transportation: at ambient temperature VT82112 Buffer Rlysis-VG 30ml Universal RPE Solution 12ml RNase-free Water 5ml EZ-10 Spin Column 50 2-ml Collection Tube 50 Rapid Animal Total RNA Extraction Kit This kit is designed for the preparation of high quality total RNA from animal tissue samples, including skeletal muscle, heart and connective tissues. Animal tissue is lysed and homogenized by Buffer Rlysis-A. RNA is then recovered by precipitation with Buffer LD. 10μg total RNA can be purified from 25mg animal tissue using this kit. Purified RNA is ready for most downstream applications such as RT-PCR, Northern Blotting, Poly (A) purification, nuclease protection and in vitro translation. 1. Simple and rapid. The entire procedure takes approximately 40 minutes. 2. High Purity of RNA. OD260/OD280 ratio of purified RNA is generally Compatible with many downstream applications, including RT-PCR, Northern Blotting, cdna synthesis, Poly (A) purification, nuclease protection and in vitro translation. 4. Easy to scale up. 5. Economic. Transportation: at ambient temperature RNA isolated from various animal tissues using the Rapid Animal Total RNA Extraction Kit. Lane 1-2: rat muscle RNA. Lane 3-4: mouse liver RNA. Lane 5-6: fish RNA. M: DNA marker AT4181 AT Solution A 50ml 250ml 1 EZ-10 Spin Column Animal Total RNA Mini-Preps Kit The EZ10 Spin Column Animal Total RNA Purification Kit provides a simple, rapid method for preparation of high-purity total RNA. The reagent contains guanidine isothiocyanate and β-mercaptoethanol that inactivate the ribonucleases present in cell extracts. RNA in the whole homogenate is selectively adsorbed on the spin column (silica membrane) while other impurities are washed away. Total RNA is eluted from the membrane by the addition of RNase-free water. 1. Fast. Using a rapid spin-column format, the entire procedure takes approx 15 minutes. 2. High Purity of RNA. OD260/OD280 ratio of purified RNA is generally > Compatible with downstream applications such as Northern Blots, cdna synthesis, RT-PCR and qrt-pcr. 4. High integrity. Buffer AG maintains the integrity of the RNA 5. Economic. Transportation: at ambient temperature BS82312 BS Buffer Rlysis-AG 25ml 125ml Universal GT Solution 18ml 90ml Universal NT Solution 6ml 30ml RNase-free Water 5ml 25ml EZ-10 Spin Column ml Collection Tube Total RNA purified using the EZ-10 Column Animal Total RNA Mini-Prep Kit. M: Marker;

35 Rapid Blood RNA Isolation Kit This kit is designed for the preparation of high quality total RNA from anticoagulated blood. 20μg total RNA can be purified from 1ml anticoagulated blood using this kit. Purified RNA is ready for most downstream applications such as RT-PCR, Northern Blotting, Poly (A) purification, nuclease protection and in vitro translation. 1. Fast. The entire procedure can be completed in 40 minutes. 2. Simple. Red Blood Cell Lysis Buffer is not required. 3. Easy to scale up. 4. Economic. Transportation: at ambient temperature BT preps BT4183 BT Buffer Rlysis-R 3ml 12ml 60ml Buffer NS-A 4.5ml 22.5ml 108ml 2% SDS Solution 0.5ml 2.5ml 12ml RNase-free Water 6ml 30ml 150ml 1 1 Total RNA was isolated from 0.75ml rabbit whole blood using Rapid Blood RNA Isolation Kit and dissolved in 20ul water. 10ul was used in formaldehyde denaturing gel electrophoresis. EZ-10 Spin Column Blood RNA Mini-Preps Kit The Blood total RNA Purification Kit provides a quick and simple method for preparation of high-purity intact total RNA. The reagent contains guanidine isothiocyanate and β-mercaptoethanol that inactivate the ribonucleases present in cell extracts. RNA in the whole homogenate is selectively adsorbed on the spin column while other impurities are washed away. Total RNA is eluted from the membrane in presence of RNasefree water. 5-15μg total RNA can be purified from 200μl anticoagulated blood using this kit. Purified RNA is ready for most downstream applications including RT-PCR, Northern Blotting, Poly (A) purification, nuclease protection and in vitro translation. 1. Fast. Using rapid spin column format, the entire procedure takes 30 minute. 2. Simple. Red Blood Cell Lysis Buffer is not required. 3. High Quality of RNA. 4. Economic. Transportation: at ambient temperature BS82313 Buffer Rlysis-R 12ml Buffer NS-A 22.5ml 2% SDS Solution 2.5ml Universal GT Solution 18ml Universal NT Solution 6ml RNase-free Water 30ml EZ-10 Column 50 2-ml Collection Tube 50 Agarose gel electrophoresis of total RNA from rat anticoagulated blood. Lane 1-3: blood total RNA. mrna Purification Kit from Total RNA This kit provides a convenient and easy-to-follow method for purification of mrna from total RNA. Each prep can use up to 10ug total RNA as starting material. mrna is selectively bound on oligo-dt cellulose, while 99% of the remaining RNA, including rrnas and trnas, do not bind. Purified mrna can be used for Norhtern blotting, RT-PCR, in vitro translation, differential display, cdna synthesis or library construction, among others. 1. Fast. The entire procedure takes only 30 minutes. 2. High Quality of mrna. OD260/OD280 of purified mrna is generally > Compatible with many downstream applications. 4. Easy to scale up. Transportation: At ambient temperature 2X Binding Buffer 1X Binding Buffer Wash Solution RNA Elution MT Preps 15ml 40ml 30ml 20ml 35

36 oligo-dt cellulose 125mg LPA 200ul RNase-free Water 10ml EZ-10 Spin Column Bacterial Total RNA Mini-Preps Kit The kit allows efficient purification of total RNA from various samples. Total RNA is easily purified from animal or human cells/tissues using a simple spin format. 5-15μg total RNA can be purified from 25mg animal tissue using this kit. Purified RNA is ready for most downstream applications such as RT- PCR, Northern Blotting, Poly(A)+ selection and in vitro translation. 1. Fast. Using rapid spin column format and fast lysis buffer the whole procedure takes less than 30 minutes. 2. High Quality of RNA. Purified RNA has an OD260/OD280 ratio of Economic. Transportation: At ambient temperature. BS88583 BS Buffer RLT 20ml 100ml DW Solution (concentrate) 36ml 180ml RPE Solution (concentrate) 112ml 60ml RNase-free Water 5ml 25ml Proteinase K 0.6 ml 3 ml EZ-10 Column ml Collection Tube Fig. 1. Total RNA purified from E. coli using the EZ10 Column Bacteria Total RNA Mini-Prep Kit. M: Marker; Lane 1-2: E. coli total RNA. RNAse Free DNAse Set Buffer RDD is optimized for on-column DNase Digestion. The buffer is also well-suited for efficient DNAase digestion in solution. DNAse is supplied in liquid form. Store at -20 C for up to 9 months. No freeze-thaw. BS88253 RNase-Free DNAse (1U/ul) Buffer RDD RNase-free Water 1.5ml 3ml 2ml EZ-10 DNAway RNA Mini-Preps Kit This kit is designed to purify RNA from small amounts of animal cells or tissues. Samples are lysed and homogenized by Buffer Lysis-DR. Genomic DNA contamination is removed using a gdna Eliminator column. Purified RNA is ready for most downstream applications including RT- PCR, Northern Blotting, Poly(A)+ selection and in vitro translation. 1. Efficient. Remove DNA during RNA purification. 2. High Quality of RNA. Purified RNA has an OD260/OD280 ratio of Fast. Entire procedure can be completed in 30 minutes. Transportation: At ambient temperature. BS88133 BS Buffer Rlysis-DR 20ml 100ml GT Solution 18ml 90ml NT Solution 6ml 30ml RNase-free Water 5ml 25ml gdna Eliminator Column

37 EZ-10 Column Gel Extraction Kit Gel Extraction Kit is an excellent tool offering a rapid and economic method to extract DNA from an agarose gel. This technology is based on binding DNA to silica-based membrane, followed by subsequent wash steps and then elute pure ready-to-use DNA. The high quality of extracted DNA can be used directly for any critical downstream application. Specification: Sampling Recovery Volume of Eluate Handling Time Up to 200 mg 70 ~ 95 % 25 ~ 50 ml Within 25 min Agarose Gel Transportation: At ambient temperature. 9K s 9K K Preps 100 Preps 200 Preps GEL 1 Solubilization Solution 10 ml 100 ml 200 ml GEL 2 Washing Solution* 3 ml 30 ml 60 ml GEL 3 Elution Solution 1 ml 10 ml 20 ml GEL Column Collection Tube * Add 7 ml / 70 ml / 140 ml of ethanol (96 ~ 100%) to GEL 2 Washing Solution when first open. PCR Purification Kit Bio Basic Inc s PCR Purification Kit is an excellent tool offering a rapid and economic method to purify DNA samples from any enzymatic reactions. This technology is based on binding DNA to silica-based membrane, followed by subsequent wash steps and then elute pure ready-to-use DNA. The very high quality purified DNA can be used directly for any critical downstream application. Specification: Sampling Recover Volume of Eluate Handling Time Up to 100 ml of PCR / Enzymatic reaction 80 ~ 95 % 25 ~ 50 ml Within 15 min Transportation: At ambient temperature. 9K s 9K K Preps 100 Preps 200 Preps PCR 1 Dilution Solution* 4 ml 40 ml 80 ml PCR 2 Washing Solution** 2 ml 20 ml 40 ml PCR 3 Elution Solution 1 ml 10 ml 20 ml GEL Column Collection Tube * Add 1.7 ml/17 ml /34 ml of ethanol (96 ~ 100%) to PCR 1 Dilution Solution when first open. ** Add 8 ml/80 ml/160 ml of ethanol (96 ~ 100%) to PCR 2 Washing Solution when first open. Plasmid DNA Miniprep Kit Bio Basic Inc s Plasmid DNA Extraction Miniprep Kit is an excellent tool offering a speed and economic method to purify plasmid DNA from bacteria cultures. This technology is based on binding DNA to silica-based membranes in chaotropic salts and washing DNA with specially formulated solutions. Compared with other harmful and time-consuming procedures, such as phenol / chloroform extraction and ethanol precipitation, Feldan Plasmid DNA Extraction Miniprep Kit shortens the handling time to about 25 min. The high quality plasmid DNA can be used directly for any downstream applications. Specification: Sampling Yield Handling Time 1~5 ml overnight culture up to 20 mg for high-copy plasmids ~25 min Transportation: At ambient temperature. 9K s 10 Preps 9K Preps MINI 1 Resuspension Solution 3 ml 30 ml 60 ml 9K Preps 37

38 MINI 2 Cell Lysis Solution 3 ml 30 ml 60 ml MINI 3 Neutralization Solution 4 ml 40 ml 80 ml MINI 4 Washing 1 Solution 3.5 ml 35 ml 70 ml (concentrated)* MINI 5 Washing 2 Solution (concentrated)** MINI 6 Elution Solution 2 ml 20 ml 40 ml RNase A (50mg/ml) 6 ml 60 ml 120 ml MINI Column Collection Tube * Add 1,5 ml/15 ml/30ml ethanol (96 ~ 100%) to MINI 4 Washing 1 Solution before first use. ** Add 8 ml/80 ml/160ml ethanol (96 ~ 100%) to MINI 5 Washing 2 Solution before first use. Genomic DNA Prep Kit Blood 9K (400 preps) / 9K (3300 preps) Note: If you are using mammalian cells from cell culture, please start with pelleted cells at step 2. It is possible to use up to 6x106 cells per prep. 1. Add 300mL of whole blood (or bone marrow) to a 1.5ml microcentrifuge tube containing 900mL of RBC Lysis Solution. Incubate for 3min. at room temperature. (Note: If blood collection occured >1 hour ago, increase the incubation time to 10min to complete cell lysis. 2. Centrifuge for 30 sec. at 13,000-16,000xg. Remove the supernatant with a pipet for leaving behind the visible white cell pellet and about 10-20mL of the residual liquid. 3. Vortex the tube vigorously for 10 sec. to resuspend the white cells in the residual liquid. (The white cells should be completely 4. Add 300mL Cell Lysis Solution to the resuspend cells and pipet up and down to release the cells. 5. Add 100mL of Protein Precipitation Solution to the cell lysate. 6. Vortex vigorously for 20 sec. to mix well. 7. Centrifuge at 13,000 ~16,000xg for 1min. The precipitated proteins should form a tight, dark brown pellet. (If the protein pellet is not tight, repeat Step 6, followed by incubation on ice for 5min then repeat Step 7.) 8. Transfer the supernatant to a clean1.5ml microcentrifuge tube containing 300mL of 100% Isopropanol (2-propanol ). 9. Mix the sample thoroughly by gentle inversions. 10. Centrifuge at 13,000-16,000xg for 1 min. (White DNA pellet will be formed.) 11. Discard the supernatant and drain tube briefly on clean absorbent paper. Add 300mL of 80% Ethanol and invert the tube several times to wash the DNA pellet. 12. Centrifuge at 13,000-16,000xg for 1 min. Carefully discard the ethanol and dry at room temperature for about 10~15 min. 13. Add 50mL~100mL of DNA Hydration Solution. 14. Vortex 5 sec. at medium speed to mix. 15. Incubate the sample at 65 C for 10~30 min. 16. Store the DNA at 4 C. (For long time storage, store sample at -20 C or -80 C). 38

39 Genomic DNA Prep Kit Bacteria 9K (400 preps) / 9K (3300 preps) Prepare 100% Isopropanol and 80% Ethanol before using kit. Please follow instruction on the provided vials to obtain recommended enzyme concentration: Lysozyme (100mg/ml) RNaseA (4mg/ml ) and store those solutions at -20 C. Preheat a bath at 37 C and 65 C for incubations. 1. Transfer 1 ml of cultured cell to a 1.5 ml microcentrifuge tube. In case cell density is low, 2ml of Cultured cells can be used. 2. Harvest the cells by centrifugation (13,000 ~16,000xg, 1 min.) and discard the supernatant. 3. Resuspend the cell pellet in 300mL of Cell Resuspension Solution. 4. Add 2mL of Lysozyme (100 mg/ml ) and mix well by inverting. 5. Incubate the tube at 37 C for 1 hour. 6. Centrifuge (13,000 ~ 16,000xg for 1 min.) and discard the supernatant. 7. Resuspend the pellet in 300mL of Cell Lysis Solution. RNase Treatment 8. Add 1.5mL of RNase A (4mg/ml) and mix by inverting. 9. Incubate at 37 C for 15 ~16 min. and cool on ice for 1 min. Protein Precipitation 10. Add 50~100mL of Protein Precipitation Solution and vortex vigorously for 20 ~ 30 sec. 11. Centrifuge at 13,000 ~16,000xg for 5 min. 12. Transfer the supernatant to a clean 1.5ml micro tube containing 300mL of 100% Isopropanol. 13. Mix the sample thoroughly by gentle inversions. 14. Centrifuge at 13,000 ~16,000xg for 1 min. (White DNA pellet will be formed.) 15. Discard the supernatant and drain tube briefly on a clean absorbent paper. Add 500mL of (80% Ethanol) and invert the tube several times to wash the DNA pellet. 16. Centrifuge at 13,000 ~ 16,000xg for 1 min. Carefully discard the Ethanol. 17. Air dry at room temperature for 10 ~15 min. DNA Hydration 18. Add 20 ~100mL of DNA Hydration Solution to the dried DNA pellet. 19. Hydrate the DNA by incubating at 65 C for 1 hour. 20. Store the DNA at 4 C. (For long time storage, store at -20 C or -80 C). Genomic DNA Prep Kit Plant 9K (400 preps) / 9K (3300 preps) Prepare Isopropanol and 80% Ethanol before using the Kit. Please follow instructions on the vial to prepare the provided RNase A solution (4mg/ml) and store at -20 o C. Prepare a bath at 65 o C for incubation. Sample collection & Handling Fresh or frozen tissue must be finely ground with a mortar and pestle in liquid nitrogen before DNA isolation. Work quickly and keep tissue cold to minimize DNAase activity. Cell Lysis 1. Transfer the finely ground tissue (10~30mg) to a 1.5ml microcentrifuge tube. 2. Add 300mL of Cell Lysis Solution to the tissue. 3. Incubate at 65 C for 60 min. mix invert the tube every 5~10 min. during the incubation. RNase Treatment 4. Add 1.5mL of RNase A (4mg/ml) to the cell lysate. 5. Mix the sample by inverting the tube 25 times and incubate at 37 C for 15~60 min. Protein Precipitation 6. Cool the sample to room temperature and add 100mL of Protein Precipitation Solution to the cell lysate. 7. Mix the solution by vortex. 8. Centrifuge at 13,000-16,000xg for 3min. (The precipitant should form a tight, green pellet. If the pellet is not tight, repeat step 7, incubate on ice for 10 min. and then repeat Step 8.) DNA Precipitation 9. Transfer supernatant to a clean 1.5ml microcentrifuge tube containing 300mL of 100% Isopropanol (2-propanol). 10. Mix the sample thoroughly by gentle inversions. 11. Centrifuge at 13,000~16,000xg for 1min (white DNA pellet will be formed). 12. Discard the supernatant and drain tube briefly on clean absorbent paper. Add 300mL of 80% Ethanol and invert tube several times to wash the DNA Pellet. 13. Centrifuge at 13,000~16,000xg for 1 min. Carefully discard the ethanol. 14. Air dry at room temperature for 10~15 min. DNA Hydration 15. Add 50mL ~100mL of DNA Hydration Solution to the dried DNA pellet. 16. Hydrate the DNA by incubating sample at 65 C for 1 hour. 17. Store DNA at 4 C (For long time storage, store at -20 C or -80 C). 39

40 Genomic DNA Prep Kit Fungi 9K (400 preps) / 9K (3300 preps) Prepare 100% Isopropanol and 80% Ethanol before using kit. Please follow instruction on the provided vials to prepare the RNase A solution (4mg/ml) and the proteinase K solution (20mg/ml) and store those vials at -20 C. Preheat a bath at 55 C for Proteinase K reaction incubation then at 65 C for RNase reaction incubation. Cell Lysis 1. Transfer 1ml of the cultured cell into a 1.5ml microcentrifuge tube. Alternatively, use mg of fungal tissue or 2-3 pieces of 0.5x1cm of Cultured plate. 2. Harvest the cell by centrifuge (13,000~16,000xg, 1 min.) and discard supernatant. 3. Resuspend the cell pellet in 300mL of Cell Resuspension Solution. 4. Add 1.5mL of Proteinase K (20mg/ml) and mix by inverting gently but thoroughly. 5. Incubate the tube at 55 o C for 1hour. 6. Centrifuge (13,000~16,000xg, 1 min.), and discard the supernatant. 7. Resuspend the pellet in 300mL of Cell Lysis Solution. Protein Precipitation 8. Add 100mL of Protein Precipitation Solution and vortex vigorously for over 20 sec. 9. Centrifuge at 13,000~16,000xg for 5 min. DNA Precipitation 10. Transfer the supernatant to a clean1.5ml microcentrifuge tube containing 300mL 100% Isopropanol (2-propanol). 11. Mix the sample by inverting gently but thoroughly. 12. Centrifuge at 13,000-16,000xg for 1 min. (white DNA pellet will be formed.) 13. Discard the supernatant and drain tube briefly on clean absorbent paper. Add 300mL of 80% Ethanol and invert the tube several times to wash the DNA pellet. 14. Centrifuge at 13,000-16,000xg for 1 min. Carefully discard the Ethanol. 15. Air dry at room temperature for 10~15 min. DNA Hydration 16. Add 50mL~100mL of DNA Hydration Solution to the dried DNA pellet. 17. Hydrate the DNA by incubating at 65 C for 1 hour. 18. Add 1.5mL of RNase A (4mg/ml) and incubate at 37 C for 60 min. 19. Store the DNA at 4 o C. (For long time storage, store at -20 C or -80 C). Genomic DNA Prep Kit Animal Tissue 9K (400 preps) / 9K (3300 preps) Prepare 100% Isopropanol and 80% Ethanol before using kit. Please follow instruction on the vials to prepare 4mg/ml RNaseA solution and 20mg/ml Proteinase K solution, then store those solutions at -20 C. Preheat a bath at 55 C for Proteinase K reaction incubation then at 65 C for RNase reaction incubation. Cell Lysis 1. Transfer 5~10mg of fresh tissue or frozen tissue to 1.5ml microcentrifuge tube. 2. Add 300ml of Cell Lysis Solution to the tissue 3. Add 1.5mL of Proteinase K (20mg/ml) to lysate and mix by inverting. Incubate at 55 C overnight or until the tissue has dissolved. Protein Precipitation 4. Cool the sample at room temperature and add 100mL of Protein Precipitation Solution to the cell lysate. 5. Mix the solution well by vortex over 20 sec. 6. Centrifuge at 13,000~16,000xg for 3 min. (The precipitated protein should form a tight pellet. If the pellet is not tight, repeat step 5, incubate on ice for 10 min. and then repeat Step 6.) DNA Precipitation 7. Transfer the supernatant to a clean 1.5ml microcentrifuge tube containing 300mL of 100% Isopropanol (2-propanol). 8. Mix the sample thoroughly by gentle inversions. 9. Centrifuge at 13,000~16,000xg for 1 min. The DNA will be visible as a pellet. 10. Discard the supernatant and drain tube briefly on clean absorbent paper. Add 300mL of 80% Ethanol and invert tube several times to wash the DNA Pellet. 11. Centrifuge at 13,000~16,000xg for 1 min. Carefully discard the ethanol. 12. Air dry at room temperature for 10~15 min. DNA Hydration 13. Add 50mL~100mL of DNA Hydration Solution to the dried DNA pellet. 14. Hydrate the DNA by incubating sample at 65 C for 1 hour. 15. Add 1.5mL of RNase A (4mg/ml) and incubate at 37 C for 30~60 min. 16. Store the DNA at 4 C (For long time storage, store at - 20 C or -80 C). 40

41 Genomic DNA Prep Kit Yeast 9K (400 preps) / 9K (3300 preps) Prepare 100% Isopropanol and 80% Ethanol before using kit. Follow instruction on the provided vials of RNase A and Lyticase vial to obtain RNase A (4mg/ml) and Lyticase (2.5U/mL) and store those vials at -20 C. Preheat a source at 65 o C for RNase reaction incubation. Cell Lysis 1. Transfer 1ml of the cultured cell into a 1.5ml microcentrifuge tube. 2. Harvest the cell by centrifuging (13,000~16,000xg, 1 min.) and discard supernatant. 3. Resuspend the cell pellet in 300mL of Cell Resuspension Solution. 4. Add 1mL of Lyticase (2.5U/mL) and mix by gentle inversions. 5. Incubate the tube at 37 C for 60 min. 6. Centrifuge (13,000~16,000g, 1 min), and discard the supernatant. 7. Resuspend the pellet in 300mL of Cell Lysis Solution. Protein Precipitation 8. Add 100mL of Protein Precipitation Solution and vortex vigorously for over 20 sec. 9. Centrifuge at 13,000~16,000xg for 5 min. DNA Precipitation 10. Transfer the supernatant to a clean1.5ml microcentrifuge tube containing 300mL of 100% Isopropanol (2-propanol). 11. Mix the sample thoroughly by gentle inversions. 12. Centrifuge at 13,000~16,000xg for 1 min. (White DNA pellet will be formed.) 13. Discard the supernatant and drain the tube briefly on clean absorbent paper. Add 300mL of 80% Ethanol and invert the tube several times to wash the DNA pellet. 14. Centrifuge at 13,000~16,000xg for 1 min. Carefully discard the ethanol. 15. Air dry at room temperature for 10~15 min. DNA Hydration 16. Add 50mL~100mL of DNA Hydration Solution to the dried DNA pellet. 17. Hydrate the DNA by incubating at 65 C for 1 hour. 18. Add 1.5mLof RNase A (4mg/ml) and incubate at 37 C for 30 min. 19. Store the DNA at 4 C. (For long time storage, store at -20 C or -80 C). Plasmid DNA Maxi Kit Bio Basic Inc s Plasmid DNA Extraction Maxiprep Kit is an excellent tool offering a rapid and economic method to purify plasmid DNA from bacteria cultures. This technology is based on alkaline lysis and purification by Anion-exchange chromatography. Compared with other harmful and time-consuming procedures such as phenol / chloroform extraction and ethanol precipitation, Bio Basic s Plasmid DNA Extraction Kit shortens the handling time to about 2 hours. The high quality plasmid DNA can be used directly for any downstream applications. Specification: Sampling Yield Handling Time 100~250 ml of bacteria culture for high copy plasmids up to 500 mg About 2 hours 200~400 ml of bacteria culture for low copy plasmids for high-copy plasmid Transportation: At ambient temperature. 9K s 9K K Preps 10 Preps 30 Preps MAXI 1 Resuspension Solution 11 ml 110 ml 3X110 ml MAXI 2 Cell Lysis Solution 11 ml 110 ml 3X110 ml MAXI 3 Neutralization Solution 11 ml 110 ml 3X110 ml MAXI 4 Equilibration Solution 13.5 ml 135 ml 3X135 ml MAXI 5 Washing Solution 55 ml 2X275 ml 6X275 ml MAXI 6 Elution Solution 13.5 ml 135 ml 3X135 ml RNase A (50 mg / ml) 22 ml 220 ml 3X220 ml MAXI Column Collection Tube Solutions provided in this kit contain irritants, wear gloves and lab coat when handling. 2. Briefly spin RNase A tube to remove drops from the inside of the lid. Transfer tube content into MAXI 1 Resuspension Solution bottle. Add 250µL of MAXI 1 Resuspension Solution into RNase A tube, rinse tube inside and transfer back into MAXI 1 Resuspension Solution bottle. Store at 4 C. 3. Check MAXI 2 Cell Lysis Solution before use. Warm MAXI 2 Cell Lysis Solution at 37 C if any precipitation formed. Prevent vigorous shaking of the MAXI 2 Cell Lysis Solution. 4. To avoid acidification of MAXI 2 Cell Lysis Solution from CO 2 in the air, close the bottle immediately after use. 41

42 Columns & 96 Well Plates Code Description Size SD5005 EZ-10 Column & collection tube (blue tube, clear ring, clear collection) 100 SD5005-EMPTY EZ-10 Empty Column without filter, without collection tube & O-ring 1000 SD5008 EZ10 RNA Column & collection tube(clear tube, clear ring, clear collection) 100 SD5003 EZ-200 Column & collection tube (set) 12 SD5004 EZ-500 Column & collection tube (set) 12 SD well filter plate (960ul each well) 12 SD5006-EMPTY 96 well filter plate (no filter) 12 SD well DNA plate with membrane (960ul each well) 12 SD well RNA plate with membrane (960ul each well) 12 SD well sample storage plate (200ul each well), 10/PK 1PK SD Well Vacuum System 1 SD5012 Medi preps or Maxi preps Column Plug 1 BR480-N 48 WELL 4ML U-SHAPED DEEP-WELL PLATES, 10/BAG 1PK BR581-96N 96 WELL 2ML DEEP PLATE, NATURAL,EDGE FILLED, 10PLATES/BAG 1PK 42