PCR Instruments and Consumables

Transcription

1 Index Page GeneAmp PCR Instrument Systems 2 GeneAmp PCR System 9700 Components 3 Accessories and Software for GeneAmp PCR Instrument Systems 4 Disposables 5 PCR Enzymes 7 GeneAmp PCR Kits 12 RNA PCR Kits 15 Other Reagents and Disposables 18 Training 20 Ordering Information 20 Trademarks 23 europe.appliedbiosystems.com Applera Italia Fillale della Applera Europe B.V. Page 1 of 23

2 GeneAmp PCR Instrument Systems Gold 96-Well GeneAmp PCR System 9700 Each 9.900,00 High performance, high speed 96-well PCR system with gold-plated silver block and graphical user interface for easy, intuitive programming. Interchangeable sample block. Includes user s manual on CD and disposables starter pack Aluminum 96-Well GeneAmp PCR System 9700 Each 7.950,00 High performance 96-well PCR system with aluminium block and graphical user interface for easy, intuitive programming. Interchangeable sample block. Includes user s manual on CD and disposables starter pack Dual 96-Well GeneAmp PCR System 9700 Each ,00 High performance, high speed 2 x 96-well PCR system with aluminium block and plate ejector and graphical user interface for easy, intuitive programming. Interchangeable sample block. Includes user s manual on CD. N Dual 384-Well GeneAmp PCR System 9700 Each ,00 High performance, high throughput 2x 384-well PCR system and graphical user interface for easy, intuitive programming. Interchangeable sample block. Includes user s manual on CD and disposables starter pack Auto-Lid Dual 384-Well GeneAmp PCR System 9700 Each ,00 High performance, high throughput 2x 384-well PCR system with automated heated lid to enable robotic sample loading and unloading. Interchangeable sample block. Includes user s manual on CD and disposables starter pack Well 0.5 ml GeneAmp PCR System 9700 Each 8.400,00 High performance 60-well PCR system for oil free PCR in 0.5 ml tubes. Graphical user interface for easy, intuitive programming and interchangeable sample block. Includes user s manual on CD and disposables. europe.appliedbiosystems.com Applera Italia Fillale della Applera Europe B.V. Page 2 of 23

3 GeneAmp PCR System 2700 Each 4.800,00 Personal-sized high performance PCR System with fixed 96-well sample block and graphical user interface for easy, intuitive programming. Includes user s manual on CD and disposables starter pack. GeneAmp PCR System 9700 Components N GeneAmp PCR System 9700 Base Module Each 7.900,00 Includes user s manual on CD. GeneAmp PCR System 9700 Base Module User s Manual, P/N , can also be downloaded from the internet as PDF file at: GeneAmp PCR System Well Gold Sample Block Each 3.540,00 Module Includes user s manual on CD. GeneAmp PCR System Well Sample Block User s Manual, P/N , can also be downloaded from the internet as PDF file at: GeneAmp PCR System Well Aluminium Sample Each 1.840,00 Block Module Includes user s manual on CD. GeneAmp PCR System Well Sample Block User s Manual, P/N , can also be downloaded from the internet as PDF file at: GeneAmp PCR System 9700 Dual 96-Well Sample Block Each 8.000,00 Module Includes user s manual on CD. User s Manual can also be downloaded from the internet as PDF file at: N GeneAmp PCR System 9700 Dual 384-Well Sample Each 8.000,00 Block Module Includes user s manual on CD. GeneAmp PCR System 9700 Dual 384-well Sample Block User s Manual, P/N , can also be downloaded from the internet as PDF file at: europe.appliedbiosystems.com Applera Italia Fillale della Applera Europe B.V. Page 3 of 23

4 GeneAmp PCR System 9700 Auto Lid Dual 384-Well Each ,00 Sample Block Module Includes user s manual on CD. GeneAmp PCR System 9700 Dual 384-well Sample Block User s Manual, P/N , can also be downloaded from the internet as PDF file at: GeneAmp PCR System Well Sample Block Each 2.400,00 Module Includes user s manual on CD. GeneAmp PCR System ml Sample Block User s Manual, P/N , can also be downloaded from the internet as PDF file at: C GeneAmp Thermal Isolation Frame Each 76,00 Accessories and Software for GeneAmp PCR Instrument Systems ml Temperature Verification Kit Each 2.740,00 For use with the 96-Well GeneAmp PCR System 9700, the GeneAmp PCR System 9600 and the GeneAmp PCR System ml Temperature Verification Kit Each 2.740,00 For use with the 60-Well GeneAmp PCR System 9700 and the DNA Thermal Cycler ml Temperature Verification Kit Each 2.740,00 For use with the Dual 384-Well GeneAmp PCR System Method Storage PCMCIA Card for 9700, 260K. Each 178,00 N Mac Communication Cable Each 7, Networking Software Kit for GeneAmp PCR System Each 1.970, Includes: Software Application CD, PCMCIA Card with Firmware, 1x 5m Communication Cable to connect Thermal Cycler with PC, 1x Serial Port Adapter, 4x Communication Cables to connect Thermal Cycler with Thermal Cycler. europe.appliedbiosystems.com Applera Italia Fillale della Applera Europe B.V. Page 4 of 23

5 Networking Software Expansion Kit for GeneAmp PCR Each 66,00 System 9700 Includes: 5 x Communication Cables to connect Thermal Cycler with Thermal Cycler. Disposables MicroAmp Disposables for the GeneAmp PCR Systems 9700, 9600, 2700 and 2400 C4 N MicroAmp Reaction Tubes with Caps 1000/Pkg 137,00 C4 N Bulkpack MicroAmp Reaction Tubes with Caps 10000/Pkg 1.250,00 C4 N MicroAmp Colored Reaction Tubes with Caps 1000/Pkg 145,00 C4 N MicroAmp Autoclaved Reaction Tubes with Caps 1000/Pkg 270,00 C4 N MicroAmp Reaction Tubes, 0.2 ml, without Caps 2000/Pkg 270,00 C4 N Bulkpack MicroAmp Reaction Tubes without Caps 10000/Pkg 1.250,00 C4 N MicroAmp Colored Reaction Tubes without Caps 1000/Pkg 137,00 C4 N MicroAmp 8 Strip Reaction Tubes, 0.2 ml, 1000/Pkg 132, Strips C4 N MicroAmp Caps, 8 Caps/Strip 300 Strips/Pkg 104,00 C4 N Bulkpack MicroAmp Caps, 8 Caps/Strip 1500 Strips/Pkg 415,00 C4 N MicroAmp 8 Strip Colored Reaction Tubes 125 Strips/Pkg 132,00 C4 N MicroAmp Colored Caps, 8 Caps/Strip 300 Strips/Pkg 111,00 C4 N MicroAmp Centrifuge Adapter Each 12,00 N MicroAmp Cap Installing Tool Each 36,00 C MicroAmp Multi Removal Tool Each 21,00 Multi-functional tool. Used for removing Microplates from a Thermal Cycler sample block; removing strip caps from Microplates and Tray/Retainer assemblies; and opening MicroAmp or GeneAmp tubes. MicroAmp Disposables for the 96-Well GeneAmp PCR Systems 9700, 9600 and 2700 C4 N MicroAmp 96-Well Reaction Plate (no barcode) 10/Pkg 76,00 C Bulkpack 96-Well Reaction Plate (50x N ) 500/Pkg 3.050,00 europe.appliedbiosystems.com Applera Italia Fillale della Applera Europe B.V. Page 5 of 23

6 C4 N MicroAmp Caps, 12 Caps/Strip 200 Strips/Pkg 104,00 C4 N Bulkpack MicroAmp Caps, 12 Caps/Strip 1000 Strips/Pkg 415,00 C4 N MicroAmp Colored Caps, 12 Caps/Strip 200 Strips/Pkg 111,00 C4 N MicroAmp 96-Well Full Plate Cover 5/Pkg 104,00 C MicroAmp 9700/9600 Reaction Tubes/Tray/Retainer 20/Pkg 540,00 Assembly C Bulkpack MicroAmp 9700/9600 Reaction 100/Pkg 2.080,00 Tubes/Tray/Retainer Assembly C Bulkpack MicroAmp 9700/9600 Reaction 1000/Pkg ,00 Tubes/Tray/Retainer Assembly C Splash Free Support Base 10/Pkg 83,00 For use on GeneAmp 9600, 9700 thermal cyclers and ABI PRISM 7700 Sequence Detection System; needs retainer to hold 0.2 ml tubes. Replaces MicroAmp Base N C4 N MicroAmp 96-Well Support Base 10/Pkg 69,00 C MicroAmp Tray/Retainer Set for 96 Tubes 10/Pkg 69,00 For use with single or strip optical tubes on the ABI PRISM 7000, 7700 and GeneAmp 5700 Sequence Detection Systems, and 310 Autosampler. C4 N MicroAmp 9600/9700 Tray for Tubes with Caps 10/Pkg 55,00 MicroAmp Disposables for the GeneAmp PCR System 2400 C4 N MicroAmp 2400 Base 16/Pkg 55,00 C4 N MicroAmp 2400 Tray/Retainer Set 16/Pkg 69,00 C4 N MicroAmp 2400 Tray for Tubes with Caps 16/Pkg 55,00 C4 N MicroAmp 2400 Full Plate Cover 5/Pkg 48,00 MicroAmp Disposables for the Dual 384-Well GeneAmp PCR System 9700 C ABI PRISM 384-well Clear Optical Reaction Plate 50/Pkg 310,00 with Barcode (code 128) C ABI PRISM 384-Well Clear Optical Reaction Plate 500/Pkg 2.620,00 with Barcode (code 128) Includes: 10 x P/N europe.appliedbiosystems.com Applera Italia Fillale della Applera Europe B.V. Page 6 of 23

7 C MicroAmp Clear Adhesive Film 100/Pkg 127,00 GeneAmp Disposables for 60-Well GeneAmp PCR System 9700 and DNA Thermal Cycler 480 C4 N GeneAmp PCR Reaction Tubes 2000/Pkg 136,00 C4 N GeneAmp Thin-Walled Reaction Tube with Flat Cap 1000/Pkg 132,00 C4 N GeneAmp Thin-Walled Reaction Tubes with Domed Cap 2000/Pkg 380,00 C4 N GeneAmp Autoclaved Thin-Walled Reaction Tubes 1000/Pkg 215,00 PCR Enzymes AmpliTaq Gold DNA Polymerase C AmpliTaq Gold PCR Master Mix 200 Rxs 320,00 Supplied at a 2x concentration. Includes 250 U AmpliTaq Gold DNA Polymerase for automated Hot Start PCR in 5ml 2x Gold PCR Buffer, 400µM each dntp and 5mM MgCl 2 for 200 x 50µl reactions, Protocol P/N The protocol can also be downloaded from the internet as PDF file at: C AmpliTaq Gold PCR Master Mix 10 x 200 Rxs 2.880,00 Includes 10 x 250U AmpliTaq Gold DNA Polymerase for automated Hot Start PCR in 2x Gold PCR Buffer, 400µM each dntp and 5mM MgCl 2 for 2000 x 50µl reactions and Protocol P/N The protocol can also be downloaded from the internet as PDF file at: C AmpliTaq Gold PCR Master Mix 2000 Rxs 2.590,00 Includes 2500 U AmpliTaq Gold DNA Polymerase for automated Hot Start PCR in 50ml 2x Gold PCR Buffer, 400µM each dntp and 5mM MgCl 2 for 2000 x 50µl reactions and Protocol P/N The protocol can also be downloaded from the internet as PDF file at: europe.appliedbiosystems.com Applera Italia Fillale della Applera Europe B.V. Page 7 of 23

8 C AmpliTaq Gold DNA Polymerase, with Gold Buffer 250 Units 214,00 Chemically modified enzyme for automated Hot Start PCR. Includes 10x PCR Gold Buffer (500mM KCl, 150mM Tris/HCl ph 8.0) and 25mM MgCl 2 solution for 200 x 50µl reactions. C AmpliTaq Gold DNA Polymerase, with Gold Buffer 1000 Units 750,00 Chemically modified enzyme for automated Hot Start PCR. Includes 10x PCR Gold Buffer and 25mM MgCl 2 solution for 800 x 50µl reactions. C Six Paq AmpliTaq GOLD DNA Polymerase, 6 x 250 Units 1.060,00 with Gold Buffer Chemically modified enzyme for automated Hot Start PCR. Includes 10x PCR Gold Buffer and 25mM MgCl 2 solution for 1200 x 50µl reactions. C Twelve Paq AmpliTaq Gold DNA Polymerase, with 12 x 250 Units 1.910,00 Gold Buffer Chemically modified enzyme for automated Hot Start PCR. Includes 10x PCR Gold Buffer and 25mM MgCl 2 solution for 2400 x50 µl reactions. C AmpliTaq Gold DNA Polymerase, with Gold Buffer 5 x 1000 Units 2.850,00 Chemically modified enzyme for automated Hot Start PCR. Includes 10x PCR Gold Buffer and 25mM MgCl 2 solution for 4000 x 50µl reactions. C AmpliTaq Gold DNA Polymerase, with Gold Buffer 25 x 1000 Units ,00 Chemically modified enzyme for automated Hot Start PCR. Includes 10x PCR Gold Buffer and 25mM MgCl 2 solution for x 50µl reactions. C AmpliTaq Gold DNA Polymerase, Bulk Pack, with 1 x Units ,00 Gold Buffer Chemically modified enzyme for automated Hot Start PCR. Includes 10x PCR Gold Buffer and 25mM MgCl 2 solution for x 50µl reactions. C1 N AmpliTaq Gold DNA Polymerase, with Buffer I 250 Units 214,00 C1 N AmpliTaq Gold DNA Polymerase, with Buffer II 250 Units 214,00 C1 N Six Paq AmpliTaq Gold DNA Polymerase, with 6 x 250 Units 1.060,00 Buffer I C1 N Six Paq AmpliTaq Gold DNA Polymerase, with 6 x 250 Units 1.060,00 Buffer II C1 N Twelve Paq AmpliTaq Gold DNA Polymerase, with 12 x 250 Units 1.910,00 Buffer I europe.appliedbiosystems.com Applera Italia Fillale della Applera Europe B.V. Page 8 of 23

9 C1 N Twelve Paq AmpliTaq Gold DNA Polymerase, with 12 x 250 Units 1.910,00 Buffer II C1 N AmpliTaq Gold DNA Polymerase, with Buffer I 1000 Units 750,00 C1 N AmpliTaq Gold DNA Polymerase, with Buffer II 1000 Units 750,00 C1 N AmpliTaq Gold DNA Polymerase, with Buffer I 5 x 1000 Units 2.850,00 C1 N AmpliTaq Gold DNA Polymerase, with Buffer II 5 x 1000 Units 2.850,00 C1 N AmpliTaq Gold DNA Polymerase 1 x Units ,00 C1 N AmpliTaq Gold DNA Polymerase, with Buffer I 1 x Units ,00 C1 N AmpliTaq Gold DNA Polymerase, with Buffer II 1 x Units ,00 C1 N AmpliTaq Gold DNA Polymerase, with Buffer I 25 x 1000 Units ,00 C1 N AmpliTaq Gold DNA Polymerase, with Buffer II 25 x 1000 Units ,00 AmpliTaq Gold DNA Polymerase Buffers C GeneAmp 10x PCR Gold Buffer and MgCl ml 27,00 Solution 500mM KCl, 150mM Tris/HCl ph 8.0 and separate 25mM MgCl 2 solution for 200 x 50µl reactions. C GeneAmp 10x PCR Gold Buffer and MgCl 2 6 x 1.5 ml 81,00 Solution, 6 set For 1200 x 50µl reactions. C GeneAmp 10x PCR Gold Buffer and MgCl ml 202,00 Solution, bulk set For x 50µl reactions. C3 N GeneAmp 10x PCR Buffer I 1.5 ml 27,00 500mM KCl, 100mM Tris/HCl ph 8.3, 15mM MgCl 2 and 0,01% (w/v) gelatin. C3 N GeneAmp 10x PCR Buffer I 6 x 1.5 ml 81,00 C3 N GeneAmp 10x PCR Buffer I 150 ml 202,00 C3 N GeneAmp 10x PCR Buffer II and MgCl ml 27,00 Solution 500mM KCl, 100mM Tris/HCl ph 8.3 and separate 25mM MgCl 2. C3 N GeneAmp 10x PCR Buffer II and MgCl 2 6 x 1.5 ml 81,00 Solution europe.appliedbiosystems.com Applera Italia Fillale della Applera Europe B.V. Page 9 of 23

10 C3 N GeneAmp 10x PCR Buffer II and MgCl ml 202,00 Solution AmpliTaq DNA Polymerase C2 N AmpliTaq DNA Polymerase, with Buffer I 250 Units 209,00 Includes 10x GeneAmp PCR Buffer I (500mM KCl, 100mM Tris/HCl ph 8.3, 15mM MgCl 2 and 0,01% (w/v) gelatin). C2 N AmpliTaq DNA Polymerase, with Buffer II 250 Units 209,00 Includes 10x GeneAmp PCR Buffer II (500mM KCl, 100mM Tris/HCl ph 8.3) and 25mM MgCl 2 solution. C2 N AmpliTaq DNA Polymerase, with Buffer I 1000 Units 735,00 C2 N AmpliTaq DNA Polymerase, with Buffer II 1000 Units 735,00 C2 N Six Paq AmpliTaq DNA Polymerase, with Buffer I 6 x 250 Units 1.040,00 C2 N Six Paq AmpliTaq DNA Polymerase, with Buffer II 6 x 250 Units 1.040,00 C2 N Twelve Paq AmpliTaq DNA Polymerase with Buffer I 12 x 250 Units 1.880,00 C2 N Twelve Paq AmpliTaq DNA Polymerase, with Buffer II 12 x 250 Units 1.880,00 C2 N AmpliTaq DNA Polymerase, with Buffer I 5 x 1000 Units 2.800,00 C2 N AmpliTaq DNA Polymerase, with Buffer II 5 x 1000 Units 2.800,00 C2 N AmpliTaq DNA Polymerase, with Buffer I 5 x 5000 Units ,00 C2 N AmpliTaq DNA Polymerase, with Buffer II 5 x 5000 Units ,00 AmpliTaq DNA Polymerase Buffers C3 N GeneAmp 10x PCR Buffer I 1.5 ml 27,00 500mM KCl, 100mM Tris/HCl ph 8.3, 15mM MgCl 2 and 0,01% (w/v) gelatin. C3 N GeneAmp 10x PCR Buffer I 6 x 1.5 ml 81,00 C3 N GeneAmp 10x PCR Buffer I 150 ml 202,00 C3 N GeneAmp 10x PCR Buffer II and MgCl ml 27,00 Solution 500mM KCl, 100mM Tris/HCl ph 8.3 and separate 25mM MgCl 2. C3 N GeneAmp 10x PCR Buffer II and MgCl 2 6 x 1.5 ml 81,00 Solution europe.appliedbiosystems.com Applera Italia Fillale della Applera Europe B.V. Page 10 of 23

11 C3 N GeneAmp 10x PCR Buffer II and MgCl ml 202,00 Solution Special PCR Enzymes C AmpliTaq Gold DNA Polymerase, LD, Kit and Protocol 250 Units 255,00 Further purified AmpliTaq Gold DNA Polymerase (5U/µl) to substantially reduce contaminating bacterial DNA sequences. Contains less than 10 copies of bacterial 16S rrna gene per 5 Unit aliquot. Includes Protocol P/N The protocol can also be downloaded from the internet as PDF file at: C AmpliTaq Gold DNA Polymerase, LD, Kit 250 Units 255,00 Further purified AmpliTaq Gold DNA Polymerase (5U/µl) to substantially reduce contaminating bacterial DNA sequences. Contains less than 10 copies of bacterial 16S rrna gene per 5 Unit aliquot. Protocol P/N can be downloaded from the internet as PDF file at: C AmpliTaq Gold LD DNA Polymerase, Kit and Protocol 1000 Units 910,00 Further purified AmpliTaq Gold DNA Polymerase (5U/µl) to substantially reduce contaminating bacterial DNA sequences. Contains less than 10 copies of bacterial 16S rrna gene per 5 Unit aliquot. Includes Protocol P/N The protocol can also be downloaded from the internet as PDF file at: C AmpliTaq Gold DNA Polymerase, LD, Kit 1000 Units 910,00 Further purified AmpliTaq Gold DNA Polymerase (5U/µl) to substantially reduce contaminating bacterial DNA sequences. Contains less than 10 copies of bacterial 16S rrna gene per 5 Unit aliquot. Protocol P/N can be downloaded from the internet as PDF file at: C2 N AmpliTaq DNA Polymerase, LD with Buffer I 250 Units 236,00 Further purified AmpliTaq DNA Polymerase (5U/µl) to substantially reduce contaminating bacterial DNA sequences. Contains less than 10 copies of bacterial 16S rrna per 5 Unit aliquot. Sufficient for 200 reactions at 5 Units / 50µl reaction. europe.appliedbiosystems.com Applera Italia Fillale della Applera Europe B.V. Page 11 of 23

12 C2 N AmpliTaq DNA Polymerase, LD with Buffer II 250 Units 236,00 Further purified AmpliTaq DNA Polymerase (5U/µl) to substantially reduce contaminating bacterial DNA sequences. Contains less than 10 copies of bacterial 16S rrna gene per 5 Unit aliquot. Sufficient for 200 reactions at 5 Units / 50µl reaction. C2 N AmpliTaq DNA Polymerase, Stoffel Fragment 1000 Units 265,00 Modified form of AmpliTaq DNA Polymerase (10U/µl) without exonuclease activity. Includes 10x Stoffel Buffer (100mM KCl, 100mM Tris/HCl ph 8.3) and separate 25mM MgCl 2. Sufficient for 200 reactions at 5 Units / 50µl reaction. C2 N Six Paq AmpliTaq DNA Polymerase, Stoffel Fragment 6 x 1000 Units 1.310,00 Modified form of AmpliTaq DNA Polymerase (10U/µl) without exonuclease activity. Includes 10x Stoffel Buffer (100mM KCl, 100mM Tris/HCl ph 8.3) and separate 25mM MgCl 2. Sufficient for 1200 reactions at 5 Units / 50µl reaction. C2 N rtth DNA Polymerase, XL and XL Buffer II 400 Units 390,00 Blend of rtth DNA Polymerase and Tli to provide optimized amounts of both 5-3 and 3-5 nuclease activity for high yields of long PCR products. Includes sufficient enzyme and buffer for 200 XL PCR amplifications, using 2 Units / 50µl reactions. C2 N Six Paq rtth DNA Polymerase, XL and XL Buffer II 6 x 400 Units 1.950,00 Includes sufficient enzyme and buffer for 1200 XL PCR amplifications, using 2 Units / 50 µl reactions. GeneAmp PCR Kits C GeneAmp Gold PCR Reagent Kit 100 Rxn 163,00 Includes 25µl AmpliTaq Gold DNA Polymerase for automated Hot Start PCR, 10x PCR Gold Buffer, 25mM MgCl 2 solution and dntp blend for 100 x 50µl reactions and Lambda Multiplex Control DNA, primers and Protocol P/N The protocol can also be downloaded from the internet as PDF file at: europe.appliedbiosystems.com Applera Italia Fillale della Applera Europe B.V. Page 12 of 23

13 C3 N GeneAmp PCR Reagent Kit with AmpliTaq DNA 200 Rxn 465,00 Polymerase Includes 250 U/50µl AmpliTaq DNA Polymerase, 1.5ml GeneAmp 10x PCR Buffer I, 4 x 320µl 10mM GeneAmp dntps for 200 x 50µl reactions and GeneAmp Lambda Control Reagents. C3 N GeneAmp PCR Core Reagents 200 Rxn 305,00 Includes 250 U/50 µl AmpliTaq DNA Polymerase, 1.5ml GeneAmp 10x PCR Buffer II, 1.5ml 25mM MgCl 2 and 4 x 320µl 10mM GeneAmp dntps for 200 x 50µl reactions. C AmpliTaq Gold PCR Master Mix 200 Rxs 320,00 Supplied at a 2x concentration. Includes 250 U AmpliTaq Gold DNA Polymerase for automated Hot Start PCR in 5ml 2x Gold PCR Buffer, 400µM each dntp and 5mM MgCl 2 for 200 x 50µl reactions, Protocol P/N The protocol can also be downloaded from the internet as PDF file at: C AmpliTaq Gold PCR Master Mix 10 x 200 Rxs 2.880,00 Includes 10 x 250U AmpliTaq Gold DNA Polymerase for automated Hot Start PCR in 2x Gold PCR Buffer, 400µM each dntp and 5mM MgCl 2 for 2000 x 50µl reactions and Protocol P/N The protocol can also be downloaded from the internet as PDF file at: C AmpliTaq Gold PCR Master Mix 2000 Rxs 2.590,00 Includes 2500 U AmpliTaq Gold DNA Polymerase for automated Hot Start PCR in 50ml 2x Gold PCR Buffer, 400µM each dntp and 5mM MgCl 2 for 2000 x 50µl reactions and Protocol P/N The protocol can also be downloaded from the internet as PDF file at: europe.appliedbiosystems.com Applera Italia Fillale della Applera Europe B.V. Page 13 of 23

14 GeneAmp High Fidelity PCR Systems C GeneAmp High Fidelity PCR System 40 Rxn 114,00 Includes 100 U GeneAmp High Fidelity Enzyme Mix, GeneAmp High Fidelity 10 X PCR Buffer with 15mM MgCl 2, GeneAmp High Fidelity 10 X PCR Buffer without MgCl 2, MgCl 2 solution, 25mM; Protocol P/N The protocol can also be downloaded from the internet as PDF file at: C GeneAmp High Fidelity PCR System 200 Rxn 540,00 Includes 2 x 250 U GeneAmp High Fidelity Enzyme Mix, GeneAmp High Fidelity 10 X PCR Buffer with 15mM MgCl 2, GeneAmp High Fidelity 10 X PCR Buffer without MgCl 2, MgCl 2 solution, 25mM; Protocol P/N The protocol can also be downloaded from the internet as PDF file at: GeneAmp XL PCR Kits C3 N GeneAmp XL PCR Kit 50 Rxn 335,00 PCR kit for extra long targets up to 40kb. Contains Blend of rtth DNA Polymerase and Tli to provide optimized polymerase and proofreading activities. Includes sufficient enzyme and buffer for 100 XL PCR amplifications, using 2 Units / 50µl reactions and Lambda Control Reagents. C3 N GeneAmp XL PCR Kit 100 Rxn 640,00 Includes sufficient enzyme and buffer for 200 XL PCR amplifications, using 2 Units / 50µl reactions and Lambda Control Reagents. PCR Kits/Contamination Control C3 N GeneAmp PCR Carry-over Prevention Kit 200 Rxn 202,00 Includes 100µl AmpErase TM Uracil N-glycosylase, 1 Unit / µl; 320µl 20mM dutp for 200 x 50µl reactions. C2 N AmpErase Uracil N-glycosylase (UNG), 100 Units 168,00 1 Unit / µl europe.appliedbiosystems.com Applera Italia Fillale della Applera Europe B.V. Page 14 of 23

15 C3 N GeneAmp dntp Blend, 12.5mM with dutp 1ml 101,00 Single vial blend, contains each datp, dctp, dgtp at 2.5mM and dutp at 5mM for 200 x 50µl reactions. RNA PCR Kits C GeneAmp AccuRT RNA PCR Kit with Controls 100 Rxn 590,00 Kit for highly accurate one-step RT-PCR. Includes: GeneAmp AccuRT enzyme (3.75 U/µL, 200 µl), 1.0 ml 5X AccuRT Buffer, 1.5 ml 25 mm Mg(OAc) 2, Control RNA (paw109) and primers sufficient for 50 reactions, 320 µl (10 mm) each of datp, dctp, dgtp and dttp, Protocol (P/N ). The protocol can also be downloaded from the internet as PDF file at: C GeneAmp AccuRT Hot Start RNA-PCR Kit with 100 Rxn 730,00 Controls Kit for highly accurate one-step RT-PCR with hot start. Includes: GeneAmp AccuRT Hot Start enzyme (3.75 U/µL, 200 µl), 1.0 ml 5X AccuRT Buffer, 1.5 ml 25 mm Mg(OAc) 2, Control RNA (paw109) and primers sufficient for 50 reactions, 320 µl (10 mm) each of datp, dctp, dgtp and dttp, protocol (P/N ). The protocol can also be downloaded from the internet as PDF file at: C GeneAmp Gold RNA PCR Reagent Kit 100 Rxn 690,00 High Sensitivity, High Specificity, One-Step RNA PCR Kit containing MultiScribe Reverse Transcriptase, AmpliTaq Gold DNA Polymerase, RNase Inhibitor, 5x RT-PCR Buffer, 100mM DTT, MgCl 2 Solution, dntp Blend, Random Hexamers, Oligo d(t) 16, GeneAmp RNA P Control Kit and Protocol P/N The protocol can also be downloaded from the internet as PDF file at: europe.appliedbiosystems.com Applera Italia Fillale della Applera Europe B.V. Page 15 of 23

16 C GeneAmp Gold RNA PCR Core Kit 100 Rxn 470,00 Contains MultiScribe Reverse Transcriptase, AmpliTaq Gold DNA Polymerase, Rnase Inhibitor, 5x RT-PCR Buffer, 100mM DTT, MgCl 2 Solution, dntp Blend, Random Hexamers, Oligo d(t) 16 and Protocol PN The protocol can also be downloaded from the internet as PDF file at: C GeneAmp RNA PCR Control Kit 20 Rxn 255,00 Contains paw109 Control RNA, Primer DM151 and Primer DM152. C3 N GeneAmp RNA PCR Kit 100 Rxn 605,00 Contains MuLV Reverse Transcriptase, AmpliTaq DNA Polymerase, Rnase Inhibitor, 10x PCR Buffer II, 100mM DTT, MgCl 2 Solution, dntps, Random Hexamers, Oligo d(t) 16 and GeneAmp RNA PCR Control Kit. C3 N GeneAmp RNA PCR Core Kit 100 Rxn 440,00 Contains MuLV Reverse Transcriptase, AmpliTaq DNA Polymerase, Rnase Inhibitor, 10x PCR Buffer II, 100mM DTT, MgCl 2 Solution, dntps, Random Hexamers and Oligo d(t) 16. C3 N GeneAmp EZ rtth RNA PCR Kit 100 Rxn 605,00 For one step RT-PCR with high temperature reverse transcription. Contains rtth DNA Polymerase, dntps, 5x EZ Buffer, 25mM Mn(OAc) 2 Solution and GeneAmp RNA PCR Control Kit. RNA PCR Enzymes C GeneAmp AccuRT RNA PCR Enzyme 100 Rxn 380,00 Enzyme for highly accurate one-step RT-PCR. Includes: GeneAmp AccuRT enzyme (3.75 U/µL, 200 µl), 1.0 ml 5X AccuRT Buffer, 1.5 ml 25 mm Mg(OAc) 2 and protocol (P/N ). The protocol can also be downloaded from the internet as PDF file at: europe.appliedbiosystems.com Applera Italia Fillale della Applera Europe B.V. Page 16 of 23

17 C GeneAmp AccuRT RNA PCR Enzyme 1000 Rxn 3.470,00 Enzyme for highly accurate one-step RT-PCR. Includes: GeneAmp AccuRT enzyme (3.75 U/µL, 200 µl), 10 X 1.0 ml 5X AccuRT Buffer, 10 X 1.5 ml 25 mm Mg(OAc) 2, protocol (P/N ). The protocol can also be downloaded from the internet as PDF file at: C GeneAmp AccuRT Hot Start RNA PCR Enzyme 100 Rxn 470,00 Enzyme for highly accurate one-step RT-PCR with hot start. Includes: GeneAmp AccuRT Hot Start enzyme (3.75 U/µL, 200 µl), 1.0 ml 5XAccuRT Buffer, 1.5 ml 25 mm Mg(OAc) 2 and protocol (P/N ). The protocol can also be downloaded from the internet as PDF file at: C GeneAmp AccuRT Hot Start RNA PCR Enzyme 1000 Rxn 4.330,00 Enzyme for highly accurate one-step RT-PCR with hot start. Includes: GeneAmp AccuRT Hot Start enzyme (3.75 U/µL, 200 µl), 10 X 1.0 ml 5X AccuRT Buffer, 10 X 1.5 ml 25 mm Mg(OAc) 2, protocol (P/N ). The protocol can also be downloaded from the internet as PDF file at: C2 N rtth DNA Polymerase and EZ Buffer Pack 500 Units 380,00 Includes rtth DNA Polymerase, 5X EZ Buffer and 25mM Mn(OAc) 2 Solution. C2 N rtth DNA Polymerase with Buffer Pack 500 Units 345,00 Includes rtth DNA Polymerase, 10x RT and Chelating Buffers, 10mM MnCl 2 and 25mM MgCl 2 Solutions. C2 N MuLV Reverse Transcriptase 5000 Units 78,00 Recombinant Moloney Murine Leukaemia Virus Reverse Transcriptase for 100 x 20µl reactions. C3 N RNase Inhibitor, 20 Units/µl, 100 reactions 2000 Units 81,00 Recombinant protein for 100 x 20µl reactions. RNA PCR Primers C3 N Random Hexamers, 100µl, 50µM. 100 Rxn 67,00 C3 N Oligo d(t) 16 Primers, 100µl, 50µM 100 Rxn 67,00 europe.appliedbiosystems.com Applera Italia Fillale della Applera Europe B.V. Page 17 of 23

18 Other Reagents and Disposables PCR dntps C3 N GeneAmp dntps 4 x 320 µl 67,00 10mM Solution of each dntp in glass-distilled water, titrated to ph 7.0 with NaOH. For 200 x 50µl reactions. C3 N GeneAmp dntp Blend with dttp 1ml 67,00 Single vial blend, contains a 10mM solution of dntp (2.5mM each of datp, dctp, dgtp and dttp) for 200 x 50µl reactions. C Six Pack GeneAmp dntp Blend, 10mM 6 x 1ml 365,00 C Twelve Pack GeneAmp dntp Blend, 10mM 12 x 1ml 645,00 C3 N GeneAmp dntp Blend, 100mM 1ml 540,00 Single vial blend, contains a 100mM solution of dntp (25mM each of datp, dctp, dgtp and dttp) for 2000 x 50µl reactions. C Twelve Pack GeneAmp dntp Blend, 100mM. 12 x 1ml 4.440,00 C3 N GeneAmp dntps with dutp 4 x 320 µl 101,00 10mM Solution of datp, dctp and dgtp and a 20mM solution of dutp. For 200 x 50µl reactions. C3 N GeneAmp dntp Blend, 12.5mM with dutp 1ml 101,00 Single vial blend, contains each datp, dctp, dgtp at 2.5mM and dutp at 5mM for 200 x 50µl reactions. AmpliWax PCR Gems and Mineral Oil C3 N AmpliWax PCR Gem Gems 135,00 Specially formulated wax beads for hot start PCR applications used in reaction volumes from 50 to 100µl. C3 N AmpliWax PCR Gem Gems 135,00 Specially formulated wax beads for hot start PCR applications used in reaction volumes from 20 to 50µl. C Mineral Oil 30 ml 20,00 europe.appliedbiosystems.com Applera Italia Fillale della Applera Europe B.V. Page 18 of 23

19 PCR Control Reagents C3 N GeneAmplimer HIV-1 Control Reagents 100 Rxn 335,00 C3 N GeneAmplimer paw109 RNA, 106 copies/µl 50 µl 101,00 DNA Sequencing Kits C3 N AmpliCycle Sequencing Kit 100 Rxn 249,00 Cycle Sequencing Kit using AmpliTaq CS which contains a point mutation virtually eliminating 5-3 exonuclease activity. DNA Preparation and Separation A PrepMan Ultra TM Sample Preparation Reagent 100 Rxn 133,00 with Protocol, 20 ml Homogeneous ready-to-use solution designed for the preparation of DNA template. For bacterial and fungal templates, DNA preparations for GMO testing and other cells. 20ml vial includes Quick Start Guide and Protocol P/N The protocol can also be downloaded from the internet as PDF file at: D Plasmid Miniprep Kit 100 Rxn 265,00 D Plasmid Miniprep Kit 500 Rxn 1.210,00 C3 N Agarose for the Separation of PCR Products 100 g 209,00 Composite Agarose blend yielding gels of high strength and flexibility. Ideal for the fine resolution of PCR products less than 1kb. In Situ PCR Consumables C3 N AmpliCover Discs 52/Pkg 78,00 C3 N AmpliCover Clips 10/Pkg 71,00 C3 N In Situ PCR Glass Slides 70/Pkg 86,00 europe.appliedbiosystems.com Applera Italia Fillale della Applera Europe B.V. Page 19 of 23

20 Training TRAIN-PCRBASIC Basic PCR Training ** 1 pers / 1 day 525,00 The course is designed for anyone, with or without previous experience, striving for successful PCR. The course includes, the basic principles of the PCR process, optimisation of reaction components and conditions, primer design, quantitative PCR, RT-PCR, long PCR, alternative enzymes and troubleshooting. **Training fees do not include travel and subsistence. Ordering Information Delivery charge per order 15,00 europe.appliedbiosystems.com Applera Italia Fillale della Applera Europe B.V. Page 20 of 23

21 For further information, to order or to obtain a quotation please contact : Applera Italia Via Tiepolo 18 I Monza (MI) Tel: Fax: Italy.Order@eur.appliedbiosystems.com Visit us at europe.appliedbiosystems.com and register at New Store Customer to get a complete overview of products, create your personal catalogue, see full pricing information, place orders and check your order status. PLEASE NOTE: Applied Biosystems markets products for specific uses. Please refer to the product label for the intended use statements of specific products. Applied Biosystems products are intended For Research Use Only. Not for use in diagnostic procedures. This price list supersedes all previous price lists and is subject to change without notice. This price list is only for distribution within Europe. All Prices Quoted Exclude V.A.T Applied Biosystems. All rights reserved. europe.appliedbiosystems.com Applera Italia Fillale della Applera Europe B.V. Page 21 of 23

22 Trademarks Applied Biosystems, AmpliCover, and MicroAmp are registered trademarks and AB (Design), Applera, and PrepMan are trademarks of Applera Corporation or its subsidiaries in the US and/or certain other countries. AmpErase, AmpliTaq, AmpliTaq Gold, AmpliWax, and GeneAmp are registered trademarks of Roche Molecular Systems, Inc. All other trademarks are the sole property of their respective owners. PLEASE NOTE: 1. Notice to Purchaser: Authorized Thermal Cylcer The GeneAmp PCR System 2700, and the GeneAmp PCR System 9700 Base Unit, in combination with its immediately attached sample block modules,comprise an instrument that is an Authorized Thermal Cycler. The purchase price of the 2700 PCR System or the 9700 PCR System Base Base Unit includes the up-front fee component of a license under United States Patent Nos. 4,683,195, 4,683,202 and 4,965,188, owned by Roche Molecular Systems, Inc., and under corresponding claims in patents outside the United States, owned by F. Hoffmann-La Roche Ltd, covering the Polymerase Chain Reaction ("PCR") process to practice the PCR process for internal research and development using this instrument. The running royalty component of that license may be purchased from Applied Biosystems or obtained by purchasing Authorized Reagents. This instrument is also an Authorized Thermal Cycler for use with applications licenses available from Applied Biosystems. Its use with Authorized Reagents also provides a limited PCR license in accordance with the label rights accompanying such reagents. Purchase of this product does not itself convey to the purchaser a complete license or right to perform the PCR process. 2. Auto-Lid Patents are pending on the Auto-Lid mechanism. 3. Notice to Purchaser: License Disclaimer: Networking Software Purchase of this software product alone does not imply any license under any process, instrument or other apparatus, system, composition, reagent or kit rights under patent claims owned or otherwise controlled by Applera Corporation, either expressly or by estoppel. 4. Notice to Purchaser: LIMITED LABEL LICENSE: AmpliTaq and AmpliTaq Gold A license under U.S. Patents 4,683,202, 4,683,195, and 4,965,188 or their foreign counterparts, owned by Roche Molecular Systems, Inc. and F. Hoffmann-La Roche Ltd (Roche), for use in research and development, has an up-front fee component and a running-royalty component. The purchase price of this product includes limited, non-transferable rights under the running-royalty component to use only this amount of the product to practice the Polymerase Chain Reaction (PCR) and related processes described in said patents solely for the research and development activities of the purchaser when this product is used in conjunction with a thermal cycler whose use is covered by the up- front fee component. Rights to the up-front fee component must be obtained by the end user in order to have a complete license. These rights under the up-front fee component may be purchased from Applied Biosystems or obtained by purchasing an authorized thermal cycler. No right to perform or offer commercial services of any kind using PCR, including without limitation reporting the results of purchaser s activities for a fee or other commercial consideration, is hereby granted by implication or estoppel. europe.appliedbiosystems.com Applera Italia Fillale della Applera Europe B.V. Page 22 of 23

23 5. Notice to Purchaser: Disclaimer of License: Other PCR Reaction Components These products are optimized for use in the polymerase chain reaction (PCR) covered by patents owned by Roche Molecular Systems, Inc. and F. Hoffmann-La Roche Ltd. No license under these patents to use the PCR Process is conveyed expressly or by implication to the purchaser by the purchase of these products. A license to use the PCR Process for certain research and development activities accompanies the purchase of certain Applied Biosystems reagents when used in conjunction with an authorized thermal cycler, or is available from Applied Biosystems. 6. Notice to Purchaser: LIMITED LICENSE: AmpliCycle Sequencing Kit A license under U.S. Patents 4,683,202, 4,683,195, 4,965,188, 5,407,800, 5,322,770 and 5,310,652 or their foreign counterparts, owned by Roche Molecular Systems, Inc. and F. Hoffmann-La Roche Ltd ("Roche"), has an up-front fee component and a running-royalty component. The purchase price of this product includes limited, nontransferable rights under the running-royalty component to use only this amount of the product to practice the Polymerase Chain Reaction ("PCR") and related processes described in said patents solely for the research and development activities of the purchaser when this product is used in conjunction with a thermal cycler whose use is covered by the up-front fee component. Rights to the up-front fee component must be obtained by the end user in order to have a complete license to use this product in the PCR process. These rights under the up-front fee component may be purchased from Applied Biosystems or obtained by purchasing an Authorized Thermal Cycler. No right to perform or offer commercial services of any kind using PCR, including without limitation reporting the results of purchaser s activities for a fee or other commercial consideration, is hereby granted by implication or estoppel. Further information on purchasing licenses to practice the PCR process may be obtained by contacting the Director of Licensing at Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California or at Roche Molecular Systems, Inc., 1145 Atlantic Avenue, Alameda, California 94501, USA. europe.appliedbiosystems.com Applera Italia Fillale della Applera Europe B.V. Page 23 of 23