N6: A Protein Crystallographic Toolbox: The CCP4 Software Suite and RCSB PDB Deposition Tools IUCr 2005 Florence Italy August 23rd
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1 N6: A Protein Crystallographic Toolbox: The CCP4 Software Suite and RCSB PDB Deposition Tools IUCr August 23rd 1
2 About this workshop The objective of this one-day workshop is not to teach crystallography but to teach people how to use some of the programs distributed with the CCP4 package to do protein crystallography, and how to get the best from the RCSB PDB when they deposit their structures. Thanks to Maeri Howard & Judy Flippen-Anderson (co-organisers) Helen Berman CCP4 and RCSB PDB The IUCr (Annalisa Guerri) and all the workshop speakers 2
3 About CCP4 CCP4 is the Collaborative Computational Project no.4 UK-based initiative Provides software for Macromolecular X-ray Crystallography Educational remit to promote good practice Core funding from BBSRC Additional income from industrial receipts Core staff based at CCLRC Daresbury Laboratory in UK Maintain, develop and release core software Make releases of the software suite Coordinate other activities including: Annual study weekend Newsletters Maintain website, bulletin board, problems pages Conferences, workshops (like this one!) 3
4 CCP4 funds other staff working on CCP4-related projects and programs across the UK Software is also provided by developers not funded by CCP4 CCP4-related software developments CCP4 Molecular Graphics (CCP4mg, Coot) Refmac5 Mosflm Phaser, Pirate, BP3, Chooch Other projects CCP4 Automation effort Involvement in various European projects: e-htpx, SPINE, BIOXHIT, PIMS 4
5 Some general CCP4 resources CCP4 website Links to lots of useful information (downloads, documentation ) Bulletin Board ccp4bb lively forum for discussing all aspects of protein crystallography Sorry for the non-ccp4 related query To (un)subscribe: Newsletters news@ccp4 Journal style newsletters Current and past issues: 5
6 The Protein Data Bank is the single worldwide repository for the processing and distribution of 3-D biological macromolecular structure data. The RCSB PDB s mission is provide the most accurate, wellannotated data in the most timely and efficient way possible to facilitate new discoveries and advances in science. As of August 1, 2005 there are more than 31,500 structures available in the archive. 6
7 Tools available for deposition and validation ADIT (deposit.rcsb.org/adit/) pdb_extract (pdb-extract.rutgers.edu/) also available through CCP4i Ligand Depot (ligand-depot.rutgers.edu/) Validation suite (deposit.rcsb.org/validate/) The RCSB PDB s new and improved search engine is available for beta testing at pdbbeta.rcsb.org 7
8 Exhibit Booths CCP4 is at stand 11 Meet CCP4 staff and developers Software demos, questions, manuals wwpdb is at booths wwpdb members are exhibiting jointly at IUCr XX RCSB PDB MSD/EBI PDBj Staff from all three sites will be available Website/software demos Please stop by for a visit! 8
9 Workshop Timetable 8:30am Introduction and Welcome Morning session: General 8:45 An Introduction to the CCP4 Package: Utilities, Tricks and Tips Peter Briggs 9:30 Overview of using CCP4 for PX Martin Noble 10:30 Coffee 11:00 Data Processing and Reduction using MOSFLM & SCALA Gwyndaf Evans 12:30 Lunch Afternoon session 13:30 TLS Refinement with REFMAC5 Martyn Winn 14:30 CCP4 Molecular Graphics: Pictures & Movies With CCP4MG Liz Potterton 15:00 Coffee 15:30 Coot And Graphical Model Building Tools Paul Emsley 16:00 Data Deposition And Validation Using RCSB PDB Tools Kyle Burkhardt 16:45 Summing Up Summary, or: "if you don't remember anything else from today, remember this..." Questions and Answers 17:00 Close 9
10 Practical points Coffee breaks at 10:30am and 3:00pm Lunch is provided by the IUCr as part of the workshop fee Packs contain copies of all the talks Please let us know if there are missing documents/other problems There are also questionnaires in the packs please give us feedback! Speakers Please please please stick to time Mr Timer will assist you Gather for photo at morning coffee 10
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