SAIL Small Animal Imaging Lab
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1 Prof. T. Helbich/PD. P. Brader SAIL Small Animal Imaging Lab
2 Small Animal Imaging - Warum? Identifikation und Entwicklung von neuen Diagnostika (Kontrastmitteln/Tracern),Therapeutika und deren Kombination (Theranostika) Longitundinale Studien in ausreichenden Fallzahl Studien an genetically engineered Tiermodellen (Transgenic, Knock-out Mäusen) Probleme mit Tieren im klinischen Krankenhausbereich Nur eingeschränkte Verfügbarkeit klinischer Scanner (Abends, Nachts oder Wochenende)
3 Small Animal Imaging - Warum? Clinical PET Scanner Resolution: 6 mm FWHM Small-animal PET Scanner Resolution: 2 mm FWHM Tumor Xenograft = Viable rim + Necrotic core Whole-Body Mouse Images Coronal sections
4 Kooperationspartner
5 Plan: SAIL im AKH-Bauteil 24, Ebene1 CeMM Anna Spiegel FG
6 Modalitäten KOMBINATION: HYBRID-SYSTEME
7 Micro PET / CT Vordere Abdeckung entfernt Δθ 1º Detector Phosphor + CCD X-ray tube 90 μm ~5-10 min/tier
8 Quantitative MicroCT Cephalo- Caudal dimension Ventro-Dorsal dimension
9 MicroPET / CT LSO single crystal - 8 pro Ring bis zu 3 Ringe FOV: 8 cm Durchmesser X 15 cm Länge Maus Ratte FWHM 1.5 mm 2.2 mm
10 MicroPET Cranial MIBG 2h Axial Coronal Sagittal Throid Gland 3.0 % ID / g Lateral Tumor Lung Tumor Liver Medial Caudal FDG 1h Gut Heart 0.0 Brain 7.0 % ID / g %ID/g B Tumor Values GLV-1h99 48h n=5 GLV-1h99 72h GLV-1h68 48h No Virus n=5 n=3 n=3 Heart Kidney 0.0 Courtesy of Peter Brader. Brader et al. Clin Cancer Res. 2009;15: h 2h 4h 12h 24h 48h
11 MicroPET / CT M M L LT M LT LT L Riedl et al. Radiology. 2008;248:
12 Optische Bildgebung Bio-Luminescence Fluorescence Requires genetic transduction - Luciferase gene - of tracked cells Conventional reflectance illumination Fluorophore source Transillumination Light originates in labeled cell Light originates exogeneously
13 Optische Bildgebung Fluoreszenz RFP GFP mplum food autofl. Multispektrale Fluoreszenzbildgebung GFP RFP Standard fluorescent image Unmixed Unmixed GFP mplum RFP mplum Unmixed Normalized Spectra Unmixed composite - Maestro Courtesy of Pat Zanzonic, MSKCC.
14 Ultraschall Spatial resolution: < 100 μ Motor-driven logitudinal motion for 3D imaging Heated stage w/ EKG sensors
15 Ultraschall
16 MicroMRT (>11 Tesla) e f cranial lateral g h L R L R i j L R L R
17 MR Portfolio
18 Kontakt: Medical Imaging Plattform /imaging
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