Lleve más, por menos.

Size: px
Start display at page:

Download "Lleve más, por menos."

Transcription

1 Lleve más, por menos. Código promo: AR1220 Código Detalle Presentación CULTIVO CELULAR AE A A A A A A mm Sodium Pyruvate Anti-Clumping Agent CD EfficientFeed C AGT Nutrient Supplement CD FortiCHO Medium CD OptiCHO Medium DMEM, High Glucose DMEM, High Glucose, GlutaMAX DMEM, high glucose, pyruvate DMEM, High Glucose, Pyruvate DMEM, high glucose, pyruvate, no glutamine DMEM, Powder, High Glucose DMEM, Powder, High Glucose, Pyruvate DMEM, Powder, High Glucose, Pyruvate DMEM, Powder, Low Glucose, Pyruvate DMEM/F-12, HEPES DMEM/F-12, Powder, HEPES DPBS, no calcium, no magnesium DPBS, Powder, no Calcium, no Magnesium EfficientFeed A+ AGT Supplement EfficientFeed C+ AGT Supplement Fetal Bovine Serum, certified, US origin Fetal Bovine Serum, certified, US origin FluoroBrite DMEM FunctionMAX TiterEnhancer Fungizone Antimycotic, Liquid Gentamicin Reagent Solution (10 mg/ml), Liquid Gentamicin Reagent Solution (50 mg/ml), Liquid Ham's F-10 Nutrient Mix Ham's F-10 Nutrient Mix, HEPES 20 ml 10x 20 ml 10 ml 10 ml

2 CULTIVO CELULAR L A Ham's F-12 Nutrient Mix Ham's F-12 Nutrient Mix, powder HEPES Buffer Solution (1 M) Kanamycin Sulfate Kanamycin Sulfate L-Glutamine-200 mm (100X), Liquid Lipofectamine 3000 Transfection Reagent Lipofectamine 2000 Transfection Reagent Lipofectamine 2000 Transfection Reagent Medium 199 MEM Non-Essential Amino Acids Solution 10 mm (100X), Liquid MEM, NEAA, no glutamine MEM, Powder, NEAA MEM, Powder, NEAA Opti-MEM Reduced Serum Medium PBS, 1X, ph 7.2 PBS, ph 7.4 Penicillin-Streptomycin, Liquid Penicillin-Streptomycin, Liquid Phytohemagglutinin (M Form) (PHA), Liquid PrestoBlue Cell Viability Reagent Qualified Fetal Bovine Serum (FBS) - Origen Nueva Zelanda Qualified Fetal Bovine Serum (FBS) - Origen Nueva Zelanda RPMI RPMI 1640 Medium, GlutaMAX Supplement RPMI, HEPES, Glutamine RPMI, no Glutamine RPMI, no Phenol Red RPMI, Powder Sf-900 II SFM TOPO TA Cloning Kit for Sequencing, without competent cells Trypan Blue Solution, 0.4% TrypLE Express (1X), no Phenol Red Trypsin 0.25% (1X), Phenol Red Trypsin-EDTA (0.05%), phenol red Trypsin-EDTA 0.25% (1X), Phenol Red Trypsin-EDTA 0.5% (10X), no Phenol Red 5 gr 25 gr 20 ml 0.75 ml 15 ml 0,75 ml 1 x 50 L 1 x 10 L 20 ml 10 ml 25 ml 10 x 25 reactions

3 PCR, RT-PCR Y qpcr N AM N mm datp 100 mm dctp 100 mm dgtp 100 mm dntp 100 mm dttp 10X BlueJuice Gel Loading Buffer AmpliTaq Gold DNA Polymerase with Gold Buffer and MgCl2 AmpliTaq Gold DNA Polymerase, with Buffer II and MgCl2 solution AMV Reverse Transcriptase, cloned ANTI-RNase (15-30 U/µL) DNase I DNase I, Amplification Grade MicroAmp Optical Tube without Cap, 0.2 ml MicroSEQ Salmonella spp. Detection Kit M-mlV Reverse Transcriptase Oligo(dT)12 18 Primer Platinum Pfx DNA Polymerase Platinum Taq DNA Polymerase - Origen Brasil Platinum Taq DNA Polymerase - Origen Brasil Platinum Taq DNA Polymerase - Origen Brasil Platinum Taq DNA Polymerase High Fidelity Platinum Pfx DNA Polymerase Power SYBR Green PCR Master Mix PrepSEQ Rapid Spin Sample Preparation Kit - Extra Clean with Proteinase K Random Primers (~297 ug/100ul) RapidFinder STEC Screening Assay RNaseOUT Recombinant Ribonuclease Inhibitor ROX Reference Dye SNaPshot Multiplex Kit SuperScript II Reverse Transcriptase SuperScript III First-Strand Synthesis System SuperScript III One-Step RT-PCR System with Platinum Taq High Fidelity SuperScript III One-Step RT-PCR System with Platinum Taq SuperScript III Platinum One-Step qrt-pcr Kit SuperScript III Reverse Transcriptase SYBR Green PCR Master Mix SYBR Select Master Mix, 1-Pack (1 x 5 ml) Taq DNA Polymerase, Native Taq DNA Polymerase, Recombinant Taq DNA Polymerase, Recombinant - Origen Brasil 4 x 3 x 1 ml 250 units 250 U 750 units 10,000 units U 100 U 2,000 tubes U 25 ug 5000 U U 250 reactions 1 x 5 ml 100 ul 0, reacc 500 ul 50 reacc 50 reacc 500 reacc 50 reacc 1 x 5 ml 500 reacc u

4 PCR, RT-PCR Y qpcr Taq DNA Polymerase, Recombinant - Origen Brasil TaqMan RNA-to-CT 1-Step Kit TaqMan STEC O103 & O145 Assay Beads, MLG TaqMan STEC O26 & O111 Assay Beads, MLG TaqMan STEC O45 & O121 Beads, MLG TaqMan Universal Master Mix II, no UNG 1-Pack (1 x 5 ml) TaqMan Universal PCR Master Mix TaqMan Universal PCR Master Mix, no AmpErase UNG TaqMan Listeria monocytogenes Detection Kit 40 reactions 96 reacc 96 reacc 96 reacc 200 reacc 1 x 5 ml 200 reacc REACTIVOS PARA SECUENCIADORES AUTOMATICOS and 31xx Running Buffer, 10X Anode Buffer Container (ABC) 3500 Series BigDye XTerminator Purification Kit - 2 ml (~ µl Reactions) BigDye Terminator v1.1 & v3.1 5X Sequencing Buffer BigDye Terminator v1.1 Cycle Sequencing Kit BigDye Terminator v3.1 Cycle Sequencing Kit Cathode Buffer Container (CBC) 3500 Series Conditioning Reagent, 3500 Series Hi-Di Formamide, 25 ml 25 ml 4 U 1 ml 4 U 1 U 25 ml POLIMEROS, MATRICES y ESTÁNDARES Y PLÁSTICOS POP-6 Polymer for the 310 Genetic Analyzer POP-7 Polymer for 3500/3500xL Genetic Analyzers POP-7 Polymer for 3500/3500xL Genetic Analyzers 47 cm 310 Capillary Array GeneScan 500 ROX Size Standard GeneScan 500 LIZ Size Standard 3 ml 384 reacc 960 samples 5 U 800 reacc 800 reacc SECUENCIACIÓN DE NUEVA GENERACIÓN (NGS) Ion 314 Chip Kit Ion 316 Chip Kit v2 8 chips 4 chips

5 PURIFICACION, CUANTIFICACION Y TINCION DE ACIDOS NUCLEICOS CS15000 AM1561 WG1201BOX NP0322BOX K K K A R11490 Q32856 Q32851 Q33211 AM7020 AM7020M AM7023 G6601 S7563 S33102 ULTRAPUROS AM AM MagnaRack Magnetic Separation Rack mirvana mirna Isolation Kit, without phenol NuPAGE Novex 10% Bis-Tris Midi Protein Gels, 12+2 well NuPAGE Novex 4-12% Bis-Tris Protein Gels, 1.0 mm, 12 well PrepSEQ Nucleic Acid Extraction Kit for Food and Environmental Testing PureLink Quick Plasmid Miniprep Kit PureLink Quick Gel Extraction and PCR Purification Combo Kit PureLink Quick Gel Extraction Kit PureLink RNA Mini Kit PureLink Viral RNA/DNA Mini Kit Quant-iT RiboGreen RNA Assay Kit Qubit Assay Tubes *set of 500* Qubit dsdna HS Assay Kit, 100 assays ng Qubit Protein Assay Kit, 100 assays * µg* RNAlater RNAlater RNA Stabilization Solution RNAlater Stabilization Solution Safe Imager 2.0 Amber Filter Replacement SYBR Green I Nucleic Acid Gel Stain-10,000X concentrate in DMSO, 500 µl SYBR Safe DNA Gel Stain D-Glucose (Dextrose) EDTA (0.5 M), ph 8.0 HEPES (Ultra Pure) MicroAmp Optical Adhesive Film SSC (20X) UltraPure Acrylamide UltraPure Agarose UltraPure Agarose UltraPure Agarose-1000 UltraPure Disodium Salt, Dihydrate (EDTA) UltraPure DNase/RNase-Free Distilled Water UltraPure Glycerol UltraPure Glycine UltraPure Glycogen 1 each 40 preps 1 box 10 gels 300 preps tubos 100 ensayos 20 x 5 ml 1 U 500 ul 400 ul 1 kg 1 kg 25 covers 100 gr 100 gr 100 ul

6 ULTRAPUROS MARCADORES CLONADO UltraPure Guanidine Hydrochloride UltraPure N,N'-Methylenebisacrylamide UltraPure Sodium Dodecyl Sulfate (SDS) UltraPure Tris UltraPure Urea Water, Distilled 1 Kb Plus DNA Ladder 100 bp DNA Ladder 100 bp DNA Ladder 50 bp DNA Ladder Low DNA Mass Ladder Gateway LR Clonase II Enzyme mix 100 gr 1 kg 250 ug 50 ug 250 ug 50 ug 50 applications 20 reacc ENZIMAS MODIFICADORAS Sal I 1,000 units T4 DNA Ligase 100 U Xba I REACTIVOS PARA EXPERIMENTOS INMUNOLÓGICOS 2,000 units C195 LC (6)-CFDA (5-(and-6)-Carboxyfluorescein Diacetate), mixed isomers Nitrocellulose Pre-Cut Blotting Membranes, 0.45 µm pore size TMB Solution (ELISA) 100 mg 20 pack lifetechnologies.com Bases y condiciones Promoción válida para la República Argentina del 10/11/2014 al 19/12/2014 inclusive o hasta agotar stock de productos, lo que ocurra primero. (1) Con la compra de 2 productos iguales (mismo código), obtiene un 20% de descuento en la segunda unidad, sobre el precio sin IVA. (2) Para acceder a la promoción deberá figurar la palabra AR1220 en el pedido. (3) Sólo participan de la promoción los productos publicados en que se encuentren en stock al momento de confirmar el pedido. Promoción no acumulable con otras promociones. Válida solamente para consumidores finales, quedando excluidos distribuidores, compras por licitaciones y clientes de industria. Procedencia de los productos: Estados Unidos, Brasil y Nueva Zelanda. Ubicación de los productos: Argentina. Invitrogen Argentina S.A., Francisco Iturri 1474, (C1427AED) CABA, Argentina. CUIT Para uso exclusivo en investigación. No recomendado para procedimientos de diagnóstico Thermo Fisher Scientific Inc.Todos los derechos reservados. Las marcas mencionadas en este documento son propiedad de Thermo Fisher Scientific Inc. o de sus respectivos propietarios.

Řekněte si o vzorky zdarma!

Řekněte si o vzorky zdarma! strana 1 z 6 Ceník platí v souladu s Obchodními podmínkami LAB MARK od 15.10.2015 Core Reagents for Molecular Biology www.bioline.com Řekněte si o vzorky zdarma! PCR ENZYME & MIXES DNA Polymerases - For

More information

Reverse Transcription System

Reverse Transcription System TECHNICAL BULLETIN Reverse Transcription System Instruc ons for use of Product A3500 Revised 1/14 TB099 Reverse Transcription System All technical literature is available on the Internet at: www.promega.com/protocols/

More information

New products in the UD- GenoMed Ltd.'s offer!

New products in the UD- GenoMed Ltd.'s offer! Plastic prducts: New prducts in the UD- GenMed Ltd.'s ffer! All assrtments f the SARSTEDT plastic prducts Cnsumable plastic labratry tls fr cell and tissue culture Bld cllectin and diagnstic prducts Medical

More information

RevertAid Premium First Strand cdna Synthesis Kit

RevertAid Premium First Strand cdna Synthesis Kit RevertAid Premium First Strand cdna Synthesis Kit #K1651, #K1652 CERTIFICATE OF ANALYSIS #K1651 Lot QUALITY CONTROL RT-PCR using 100 fg of control GAPDH RNA and GAPDH control primers generated a prominent

More information

PCR Instruments and Consumables

PCR Instruments and Consumables Index Page GeneAmp PCR Instrument Systems 2 GeneAmp PCR System 9700 Components 3 Accessories and Software for GeneAmp PCR Instrument Systems 4 Disposables 5 PCR Enzymes 7 GeneAmp PCR Kits 12 RNA PCR Kits

More information

TIANquick Mini Purification Kit

TIANquick Mini Purification Kit TIANquick Mini Purification Kit For purification of PCR products, 100 bp to 20 kb www.tiangen.com TIANquick Mini Purification Kit (Spin column) Cat no. DP203 Kit Contents Contents Buffer BL Buffer PB Buffer

More information

Updated: July 2005. 5' End label RNA markers 18.113 (18mer) and 44.12 (24mer) with Kinase and 32P-gamma-ATP. Gel purify labeled markers.

Updated: July 2005. 5' End label RNA markers 18.113 (18mer) and 44.12 (24mer) with Kinase and 32P-gamma-ATP. Gel purify labeled markers. RNA Cloning Method Flowchart Extract total RNA containing small RNAs. Check quality on denaturing gel with EtBr staining 5' End label RNA markers 18.113 (18mer) and 44.12 (24mer) with Kinase and 32P-gamma-ATP.

More information

In vitro analysis of pri-mirna processing. by Drosha-DGCR8 complex. (Narry Kim s lab)

In vitro analysis of pri-mirna processing. by Drosha-DGCR8 complex. (Narry Kim s lab) In vitro analysis of pri-mirna processing by Drosha-DGCR8 complex (Narry Kim s lab) 1-1. Preparation of radiolabeled pri-mirna transcript The RNA substrate for a cropping reaction can be prepared by in

More information

PCR was carried out in a reaction volume of 20 µl using the ABI AmpliTaq GOLD kit (ABI,

PCR was carried out in a reaction volume of 20 µl using the ABI AmpliTaq GOLD kit (ABI, Supplemental Text/Tables PCR Amplification and Sequencing PCR was carried out in a reaction volume of 20 µl using the ABI AmpliTaq GOLD kit (ABI, Foster City, CA). Each PCR reaction contained 20 ng genomic

More information

Laboratory Techniques I Oncology for Scientists I

Laboratory Techniques I Oncology for Scientists I Laboratory Techniques I Oncology for Scientists I September 9 th, 2015 Hayley Affronti, PhD Student Hayley.Affronti@roswellpark.org Dr. Sheila Figel too! When we first hit the lab there are so many things

More information

RT-PCR: Two-Step Protocol

RT-PCR: Two-Step Protocol RT-PCR: Two-Step Protocol We will provide both one-step and two-step protocols for RT-PCR. We recommend the twostep protocol for this class. In the one-step protocol, the components of RT and PCR are mixed

More information

RT31-020 20 rxns. RT31-100 100 rxns TRANSCRIPTME Enzyme Mix (1) 40 µl 2 x 50 µl 5 x 40 µl

RT31-020 20 rxns. RT31-100 100 rxns TRANSCRIPTME Enzyme Mix (1) 40 µl 2 x 50 µl 5 x 40 µl Components RT31-020 20 rxns RT31-050 50 rxns RT31-100 100 rxns TRANSCRIPTME Enzyme Mix (1) 40 µl 2 x 50 µl 5 x 40 µl 2x RT Master Mix (2) 200 µl 2 x 250 µl 5 x 200 µl RNase H (E. coli) 20 µl 2 x 25 µl

More information

BUFFERS and MEDIAS Coomassie Blue Staining Solution Coomassie blue Destaining Solution DMEM Normal Cell Culture Media

BUFFERS and MEDIAS Coomassie Blue Staining Solution Coomassie blue Destaining Solution DMEM Normal Cell Culture Media BUFFERS and MEDIAS Coomassie Blue Staining Solution 2 g Coomassie Blue 2 L Methanol or Ethanol * 1.6 L 400 ml Glacial acetic acid *If you will be microwaving the gel in staining solution for rapid staining

More information

Chromatin Immunoprecipitation (ChIP)

Chromatin Immunoprecipitation (ChIP) Chromatin Immunoprecipitation (ChIP) Day 1 A) DNA shearing 1. Samples Dissect tissue (One Mouse OBs) of interest and transfer to an eppendorf containing 0.5 ml of dissecting media (on ice) or PBS but without

More information

First Strand cdna Synthesis

First Strand cdna Synthesis 380PR 01 G-Biosciences 1-800-628-7730 1-314-991-6034 technical@gbiosciences.com A Geno Technology, Inc. (USA) brand name First Strand cdna Synthesis (Cat. # 786 812) think proteins! think G-Biosciences

More information

Thermo Scientific DyNAmo cdna Synthesis Kit for qrt-pcr Technical Manual

Thermo Scientific DyNAmo cdna Synthesis Kit for qrt-pcr Technical Manual Thermo Scientific DyNAmo cdna Synthesis Kit for qrt-pcr Technical Manual F- 470S 20 cdna synthesis reactions (20 µl each) F- 470L 100 cdna synthesis reactions (20 µl each) Table of contents 1. Description...

More information

Human Tubal Fluid (HTF) Media & Modifi ed Human Tubal Fluid (mhtf) Medium with Gentamicin

Human Tubal Fluid (HTF) Media & Modifi ed Human Tubal Fluid (mhtf) Medium with Gentamicin Human Tubal Fluid (HTF) Media & Modifi ed Human Tubal Fluid (mhtf) Medium with Gentamicin HTF Media are intended for use in assisted reproductive procedures which include gamete and embryo manipulation

More information

Qubit dsdna HS Assay Kits

Qubit dsdna HS Assay Kits Qubit dsdna HS Assay Kits For use with the Qubit 2.0 Fluorometer Table 1. Contents and storage information. Material Amount Concentration Storage Stability Qubit dsdna HS Reagent (Component A) 250 µl or

More information

All-in-One First-Strand cdna Synthesis Kit

All-in-One First-Strand cdna Synthesis Kit All-in-One First-Strand cdna Synthesis Kit For reliable first-strand cdna synthesis from all RNA sources Cat. No. AORT-0020 (20 synthesis reactions) Cat. No. AORT-0050 (50 synthesis reactions) User Manual

More information

Real-Time Quantitative PCR Instruments and Consumables

Real-Time Quantitative PCR Instruments and Consumables Index Page Sequence Detection Systems 2 Sequence Detection Systems Accessories and Softwares 3 Sequence Detection PCR Reagent Kits 4 Sequence Detection PCR Reagents 9 Sequence Detection Systems Disposables

More information

Genomic DNA Clean & Concentrator Catalog Nos. D4010 & D4011

Genomic DNA Clean & Concentrator Catalog Nos. D4010 & D4011 Page 0 INSTRUCTION MANUAL Catalog Nos. D4010 & D4011 Highlights Quick (5 minute) spin column recovery of large-sized DNA (e.g., genomic, mitochondrial, plasmid (BAC/PAC), viral, phage, (wga)dna, etc.)

More information

Table of Contents. I. Description... 2. II. Kit Components... 2. III. Storage... 2. IV. 1st Strand cdna Synthesis Reaction... 3

Table of Contents. I. Description... 2. II. Kit Components... 2. III. Storage... 2. IV. 1st Strand cdna Synthesis Reaction... 3 Table of Contents I. Description... 2 II. Kit Components... 2 III. Storage... 2 IV. 1st Strand cdna Synthesis Reaction... 3 V. RT-PCR, Real-time RT-PCR... 4 VI. Application... 5 VII. Preparation of RNA

More information

Path-ID Multiplex One-Step RT-PCR Kit

Path-ID Multiplex One-Step RT-PCR Kit USER GUIDE Path-ID Multiplex One-Step RT-PCR Kit TaqMan probe-based multiplex one-step real-time RT-PCR detection of RNA targets Catalog Numbers 4428206, 4428207, 4440022 Publication Part Number 4440907

More information

PyroPhage 3173 DNA Polymerase, Exonuclease Minus (Exo-)

PyroPhage 3173 DNA Polymerase, Exonuclease Minus (Exo-) PyroPhage 3173 DNA Polymerase, Exonuclease Minus (Exo-) FOR RESEARCH USE ONLY. NOT FOR HUMAN OR DIAGNOSTIC USE Lucigen Corporation 2905 Parmenter St, Middleton, WI 53562 USA Toll Free: (888) 575-9695 (608)

More information

Troubleshooting Sequencing Data

Troubleshooting Sequencing Data Troubleshooting Sequencing Data Troubleshooting Sequencing Data No recognizable sequence (see page 7-10) Insufficient Quantitate the DNA. Increase the amount of DNA in the sequencing reactions. See page

More information

MMLV High Performance Reverse Transcriptase

MMLV High Performance Reverse Transcriptase MMLV High Performance Reverse Transcriptase Cat. Nos. RT80110K and RT80125K Connect with Epicentre on our blog (epicentral.blogspot.com), Facebook (facebook.com/epicentrebio), and Twitter (@EpicentreBio).

More information

HiPer RT-PCR Teaching Kit

HiPer RT-PCR Teaching Kit HiPer RT-PCR Teaching Kit Product Code: HTBM024 Number of experiments that can be performed: 5 Duration of Experiment: Protocol: 4 hours Agarose Gel Electrophoresis: 45 minutes Storage Instructions: The

More information

All-in-One mirna qrt-pcr Reagent Kits For quantitative detection of mature mirna

All-in-One mirna qrt-pcr Reagent Kits For quantitative detection of mature mirna All-in-One mirna qrt-pcr Reagent Kits For quantitative detection of mature mirna All-in-One TM mirna First-Strand cdna Synthesis Kit AMRT-0020 (20 RT reactions), AMRT-0060 (60 RT reactions) Used in combination

More information

Real-time quantitative RT -PCR (Taqman)

Real-time quantitative RT -PCR (Taqman) Real-time quantitative RT -PCR (Taqman) Author: SC, Patti Lab, 3/03 This is performed as a 2-step reaction: 1. cdna synthesis from DNase 1-treated total RNA 2. PCR 1. cdna synthesis (Advantage RT-for-PCR

More information

TECHNICAL BULLETIN. HIS-Select Nickel Affinity Gel. Catalog Number P6611 Storage Temperature 2 8 C

TECHNICAL BULLETIN. HIS-Select Nickel Affinity Gel. Catalog Number P6611 Storage Temperature 2 8 C HIS-Select Nickel Affinity Gel Catalog Number P6611 Storage Temperature 2 8 C TECHNICAL BULLETIN Product Description HIS-Select Nickel Affinity Gel is an immobilized metalion affinity chromatography (IMAC)

More information

Mir-X mirna First-Strand Synthesis Kit User Manual

Mir-X mirna First-Strand Synthesis Kit User Manual User Manual Mir-X mirna First-Strand Synthesis Kit User Manual United States/Canada 800.662.2566 Asia Pacific +1.650.919.7300 Europe +33.(0)1.3904.6880 Japan +81.(0)77.543.6116 Clontech Laboratories, Inc.

More information

ab185916 Hi-Fi cdna Synthesis Kit

ab185916 Hi-Fi cdna Synthesis Kit ab185916 Hi-Fi cdna Synthesis Kit Instructions for Use For cdna synthesis from various RNA samples This product is for research use only and is not intended for diagnostic use. Version 1 Last Updated 1

More information

ID kit. imegen Anchovies II. and E. japonicus) DNA detection by. User manual. Anchovies species (E. encrasicolus. sequencing.

ID kit. imegen Anchovies II. and E. japonicus) DNA detection by. User manual. Anchovies species (E. encrasicolus. sequencing. User manual imegen Anchovies II ID kit Anchovies species (E. encrasicolus and E. japonicus) DNA detection by sequencing Reference: Made in Spain The information in this guide is subject to change without

More information

Power SYBR Green PCR Master Mix and Power SYBR Green RT-PCR Reagents Kit

Power SYBR Green PCR Master Mix and Power SYBR Green RT-PCR Reagents Kit USER GUIDE Power SYBR Green PCR Master Mix and Power SYBR Green RT-PCR Reagents Kit Catalog Number 4368577, 4367659, 4367660, 4368706, 4368702, 4368708 (Master Mix) and 4368711 (RT-PCR Reagents Kit) Publication

More information

Molecular Biology Techniques: A Classroom Laboratory Manual THIRD EDITION

Molecular Biology Techniques: A Classroom Laboratory Manual THIRD EDITION Molecular Biology Techniques: A Classroom Laboratory Manual THIRD EDITION Susan Carson Heather B. Miller D.Scott Witherow ELSEVIER AMSTERDAM BOSTON HEIDELBERG LONDON NEW YORK OXFORD PARIS SAN DIEGO SAN

More information

AffinityScript QPCR cdna Synthesis Kit

AffinityScript QPCR cdna Synthesis Kit AffinityScript QPCR cdna Synthesis Kit INSTRUCTION MANUAL Catalog #600559 Revision C.01 For In Vitro Use Only 600559-12 LIMITED PRODUCT WARRANTY This warranty limits our liability to replacement of this

More information

Taq98 Hot Start 2X Master Mix

Taq98 Hot Start 2X Master Mix Taq98 Hot Start 2X Master Mix Optimized for 98C Denaturation Lucigen Corporation 2905 Parmenter St, Middleton, WI 53562 USA Toll Free: (888) 575-9695 (608) 831-9011 FAX: (608) 831-9012 lucigen@lucigen.com

More information

ArC Amine Reactive Compensation Bead Kit

ArC Amine Reactive Compensation Bead Kit ArC Amine Reactive Compensation Bead Kit Catalog no. A1346 Table 1. Contents and storage information. Material Amount Composition Storage Stability ArC reactive beads (Component A) ArC negative beads (Component

More information

All-in-One mirna qrt-pcr Detection System Handbook

All-in-One mirna qrt-pcr Detection System Handbook All-in-One mirna qrt-pcr Detection System Handbook For quantitative detection of mature mirna All-in-One mirna First-Strand cdna Synthesis Kit Cat. No. AMRT-0020 (20 mirna reverse transcription reactions)

More information

Chromatin Immunoprecipitation

Chromatin Immunoprecipitation Chromatin Immunoprecipitation A) Prepare a yeast culture (see the Galactose Induction Protocol for details). 1) Start a small culture (e.g. 2 ml) in YEPD or selective media from a single colony. 2) Spin

More information

How To Make A Tri Reagent

How To Make A Tri Reagent TRI Reagent For processing tissues, cells cultured in monolayer or cell pellets Catalog Number T9424 Store at room temperature. TECHNICAL BULLETIN Product Description TRI Reagent is a quick and convenient

More information

Recipes for Reagents and Stock Solutions

Recipes for Reagents and Stock Solutions The success of the laboratories depends on the use of high-quality reagents. Follow the recipes with care and pay attention to cleanliness. Use a clean spatula for each ingredient or carefully pour each

More information

RIBOPROTECT. RNase Inhibitor RT33-020, RT33-100

RIBOPROTECT. RNase Inhibitor RT33-020, RT33-100 RIBOPROTECT RT33-020, RT33-100 RT33-020, RT33-100 RIBOPROTECT The RIBOPROTECT is a recombinant protein isolated and purified from Escherichia coli. It inhibits ribonuclease (RNase) activity of enzymes

More information

ZR-96 DNA Sequencing Clean-up Kit Catalog Nos. D4052 & D4053

ZR-96 DNA Sequencing Clean-up Kit Catalog Nos. D4052 & D4053 INSTRUCTION MANUAL ZR-96 DNA Sequencing Clean-up Kit Catalog Nos. D4052 & D4053 Highlights Simple 10 Minute Bind, Wash, Elute Procedure Flexible 15-20 µl Elution Volumes Allow for Direct Loading of Samples

More information

Sanger Sequencing. Troubleshooting Guide. Failed sequence

Sanger Sequencing. Troubleshooting Guide. Failed sequence Sanger Sequencing Troubleshooting Guide Below are examples of the main problems experienced in ABI Sanger sequencing. Possible causes for failure and their solutions are listed below each example. The

More information

TaqMan Fast Advanced Master Mix. Protocol

TaqMan Fast Advanced Master Mix. Protocol TaqMan Fast Advanced Master Mix Protocol For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use. Information in this document is subject to change without notice. APPLIED

More information

CompleteⅡ 1st strand cdna Synthesis Kit

CompleteⅡ 1st strand cdna Synthesis Kit Instruction Manual CompleteⅡ 1st strand cdna Synthesis Kit Catalog # GM30401, GM30402 Green Mountain Biosystems. LLC Web: www.greenmountainbio.com Tel: 800-942-1160 Sales: Sales@ greenmountainbio.com Support:

More information

DNA Sequencing Setup and Troubleshooting

DNA Sequencing Setup and Troubleshooting DNA Sequencing Setup and Troubleshooting Lara Cullen, PhD Scientific Applications Specialist Australia and New Zealand Reviewing Sequencing Data Review the Electropherogram Review the Raw Data (Signal

More information

Speed Matters - Fast ways from template to result

Speed Matters - Fast ways from template to result qpcr Symposium 2007 - Weihenstephan Speed Matters - Fast ways from template to result March 28, 2007 Dr. Thorsten Traeger Senior Scientist, Research and Development - 1 - Overview Ạgenda Fast PCR The Challenges

More information

Global MicroRNA Amplification Kit

Global MicroRNA Amplification Kit Global MicroRNA Amplification Kit Store kit at -20 C on receipt (ver. 3-060901) A limited-use label license covers this product. By use of this product, you accept the terms and conditions outlined in

More information

Genolution Pharmaceuticals, Inc. Life Science and Molecular Diagnostic Products

Genolution Pharmaceuticals, Inc. Life Science and Molecular Diagnostic Products Genolution Pharmaceuticals, Inc. Revolution through genes, And Solution through genes. Life Science and Molecular Diagnostic Products www.genolution1.com TEL; 02-3010-8670, 8672 Geno-Serum Hepatitis B

More information

Sequencing Guidelines Adapted from ABI BigDye Terminator v3.1 Cycle Sequencing Kit and Roswell Park Cancer Institute Core Laboratory website

Sequencing Guidelines Adapted from ABI BigDye Terminator v3.1 Cycle Sequencing Kit and Roswell Park Cancer Institute Core Laboratory website Biomolecular Core Facility AI Dupont Hospital for Children, Rockland Center One, Room 214 Core: (302) 651-6712, Office: (302) 651-6707, mbcore@nemours.org Katia Sol-Church, Ph.D., Director Jennifer Frenck

More information

QUANTITATIVE RT-PCR. A = B (1+e) n. A=amplified products, B=input templates, n=cycle number, and e=amplification efficiency.

QUANTITATIVE RT-PCR. A = B (1+e) n. A=amplified products, B=input templates, n=cycle number, and e=amplification efficiency. QUANTITATIVE RT-PCR Application: Quantitative RT-PCR is used to quantify mrna in both relative and absolute terms. It can be applied for the quantification of mrna expressed from endogenous genes, and

More information

biotechrabbit Product Catalog

biotechrabbit Product Catalog biotechrabbit Product Catalog 2014 2015 biotechrabbit GmbH Neuendorfstr. 24a 16761 Hennigsdorf, Germany info@biotechrabbit.com www.biotechrabbit.com Office: +49 3302 20754 1 0 Fax: +49 3302 20754 1 1 Commercial

More information

Essentials of Real Time PCR. About Sequence Detection Chemistries

Essentials of Real Time PCR. About Sequence Detection Chemistries Essentials of Real Time PCR About Real-Time PCR Assays Real-time Polymerase Chain Reaction (PCR) is the ability to monitor the progress of the PCR as it occurs (i.e., in real time). Data is therefore collected

More information

DNA Sequence Analysis

DNA Sequence Analysis DNA Sequence Analysis Two general kinds of analysis Screen for one of a set of known sequences Determine the sequence even if it is novel Screening for a known sequence usually involves an oligonucleotide

More information

ONLINE SUPPLEMENTAL MATERIAL. Allele-Specific Expression of Angiotensinogen in Human Subcutaneous Adipose Tissue

ONLINE SUPPLEMENTAL MATERIAL. Allele-Specific Expression of Angiotensinogen in Human Subcutaneous Adipose Tissue ONLINE SUPPLEMENTAL MATERIAL Allele-Specific Expression of Angiotensinogen in Human Subcutaneous Adipose Tissue Sungmi Park 1, Ko-Ting Lu 1, Xuebo Liu 1, Tapan K. Chatterjee 2, Steven M. Rudich 3, Neal

More information

SYBR Green PCR Master Mix and SYBR Green RT-PCR Reagents Kit

SYBR Green PCR Master Mix and SYBR Green RT-PCR Reagents Kit USER GUIDE SYBR Green PCR Master Mix and SYBR Green RT-PCR Reagents Kit Catalog Number 4309155 (Master Mix) and 4306736 (RT-PCR Reagents Kit) Publication Part Number 4310251 Rev. G Revision Date September

More information

ZR DNA Sequencing Clean-up Kit

ZR DNA Sequencing Clean-up Kit INSTRUCTION MANUAL ZR DNA Sequencing Clean-up Kit Catalog Nos. D40 & D4051 Highlights Simple 2 Minute Bind, Wash, Elute Procedure Flexible 6-20 µl Elution Volumes Allow for Direct Loading of Samples with

More information

DyNAmo cdna Synthesis Kit for qrt-pcr

DyNAmo cdna Synthesis Kit for qrt-pcr DyNAmo cdna Synthesis Kit for qrt-pcr Instruction manual F- 470S Sufficient for 20 cdna synthesis reactions (20 µl each) F- 470L Sufficient for 100 cdna synthesis reactions (20 µl each) Description...

More information

Expresión de la información genética en eucariotas. similitudes y diferencias con procariotas. metodologías de estudio 2

Expresión de la información genética en eucariotas. similitudes y diferencias con procariotas. metodologías de estudio 2 Expresión de la información genética en eucariotas similitudes y diferencias con procariotas metodologías de estudio 2 Víctor Romanowski, 2013 Polymerase chain reaction (PCR) The polymerase chain reaction

More information

FOR REFERENCE PURPOSES

FOR REFERENCE PURPOSES BIOO LIFE SCIENCE PRODUCTS FOR REFERENCE PURPOSES This manual is for Reference Purposes Only. DO NOT use this protocol to run your assays. Periodically, optimizations and revisions are made to the kit

More information

Znak sprawy: ZP-PNU/D/2014/4/42

Znak sprawy: ZP-PNU/D/2014/4/42 Znak sprawy: ZP-PNU/D/2014/4/42 Załącznik nr 3 - Formularz cenowy SPECYFIKACJA PRZEDMIOTU ZAMÓWIENIA w postępowaniu o udzielenie zamówienia publicznego prowadzonym w trybie przetargu nieograniczonego na:

More information

Optimice sus purificaciones de ADN y ARN con nuestros kits Stratagene y el Bioanalizador 2100

Optimice sus purificaciones de ADN y ARN con nuestros kits Stratagene y el Bioanalizador 2100 Optimice sus purificaciones de ADN y ARN con nuestros kits Stratagene y el Bioanalizador 2100 Indice 1-Introducción a la purificación del ADN A- kits de extracción B- Control de Calidad 2-Introducción

More information

Western Blotting: Mini-gels

Western Blotting: Mini-gels Western Blotting: Mini-gels Materials a Protein Extraction Buffer (for callus or kernel), Solution Stock Final Volume Tris-HCl ph 80 1 M 200 mm 20 ml NaCl 4 M 100 mm 25 ml Sucrose 2 M 400 mm 20 ml EDTA

More information

How To Write An Ipa

How To Write An Ipa LIBRARY PREPARATION NEBNext Multiplex Small RNA Library Prep Set for Illumina (Set 1) Instruction Manual NEB #E7300S/L 24/96 reactions Sign up for the NEBNext e-newsletter Scan this code or visit www.neb.com/

More information

Peptide Antibody Production

Peptide Antibody Production Peptide Antibody Production A) Peptide BioSynthesis (http://www.biosyn.com, 800-227-0627) B) Conjugation of peptide to KLH (Imject Maleimide Activated KLH, PIERCE=Thermo #77605, 10 mg) C) Peptide affinity

More information

Southern Blot Analysis (from Baker lab, university of Florida)

Southern Blot Analysis (from Baker lab, university of Florida) Southern Blot Analysis (from Baker lab, university of Florida) DNA Prep Prepare DNA via your favorite method. You may find a protocol under Mini Yeast Genomic Prep. Restriction Digest 1.Digest DNA with

More information

mircute mirna qpcr Detection Kit (SYBR Green)

mircute mirna qpcr Detection Kit (SYBR Green) mircute mirna qpcr Detection Kit (SYBR Green) For detection of mirna using real-time RT-PCR (SYBR Green I) www.tiangen.com QP110302 mircute mirna qpcr Detection Kit (SYBR Green) Kit Contents Cat. no. FP401

More information

Introduction To Real Time Quantitative PCR (qpcr)

Introduction To Real Time Quantitative PCR (qpcr) Introduction To Real Time Quantitative PCR (qpcr) SABiosciences, A QIAGEN Company www.sabiosciences.com The Seminar Topics The advantages of qpcr versus conventional PCR Work flow & applications Factors

More information

The cell lines used in this study were obtained from the American Type Culture

The cell lines used in this study were obtained from the American Type Culture Supplementary materials and methods Cell culture and drug treatments The cell lines used in this study were obtained from the American Type Culture Collection (ATCC) and grown as previously described.

More information

Absolute Quantification Getting Started Guide

Absolute Quantification Getting Started Guide 5 cdna Reverse Primer Oligo d(t) or random hexamer Synthesis of 1st cdna strand 3 5 cdna Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Absolute Quantification Getting Started Guide Introduction

More information

Investigating the role of a Cryptosporidium parum apyrase in infection

Investigating the role of a Cryptosporidium parum apyrase in infection Investigating the role of a Cryptosporidium parum apyrase in infection David Riccardi and Patricio Manque Abstract This project attempted to characterize the function of a Cryptosporidium parvum apyrase

More information

Dongsheng Biotech Co., Ltd. was established in 2005 in Guangzhou China.

Dongsheng Biotech Co., Ltd. was established in 2005 in Guangzhou China. Dongsheng Biotech Co., Ltd. was established in 2005 in Guangzhou China. We focus on R&D and produce fundamental molecular biology reagents. In the past 5 years, Dongsheng Biotech has successfully grown

More information

Introduction. Preparation of Template DNA

Introduction. Preparation of Template DNA Procedures and Recommendations for DNA Sequencing at the Plant-Microbe Genomics Facility Ohio State University Biological Sciences Building Room 420, 484 W. 12th Ave., Columbus OH 43210 Telephone: 614/247-6204;

More information

MANAGEMENT & DISPOSAL OF QIAGEN REAGENTS

MANAGEMENT & DISPOSAL OF QIAGEN REAGENTS Buffer AE Buffer AL Buffer ALO Buffer AP1 Buffer AP2 Buffer AP3/E Buffer APP Buffer ATL Buffer AW Buffer AW1 Buffer AW2 Buffer BB Buffer EB Buffer EC Buffer ETR Buffer EX Reaction Buffer Buffer N3 Buffer

More information

UltraClean PCR Clean-Up Kit

UltraClean PCR Clean-Up Kit UltraClean PCR Clean-Up Kit Catalog No. Quantity 12500-50 50 Preps 12500-100 100 Preps 12500-250 250 Preps Instruction Manual Please recycle Version: 02212013 1 Table of Contents Introduction... 3 Protocol

More information

QIAGEN OneStep RT-PCR Handbook

QIAGEN OneStep RT-PCR Handbook October 2012 QIAGEN OneStep RT-PCR Handbook For fast and highly sensitive one-step RT-PCR Sample & Assay Technologies QIAGEN Sample and Assay Technologies QIAGEN is the leading provider of innovative sample

More information

Transformation Protocol

Transformation Protocol To make Glycerol Stocks of Plasmids ** To be done in the hood and use RNase/DNase free tips** 1. In a 10 ml sterile tube add 3 ml autoclaved LB broth and 1.5 ul antibiotic (@ 100 ug/ul) or 3 ul antibiotic

More information

GENOTYPING ASSAYS AT ZIRC

GENOTYPING ASSAYS AT ZIRC GENOTYPING ASSAYS AT ZIRC A. READ THIS FIRST - DISCLAIMER Dear ZIRC user, We now provide detailed genotyping protocols for a number of zebrafish lines distributed by ZIRC. These protocols were developed

More information

Classic Immunoprecipitation

Classic Immunoprecipitation 292PR 01 G-Biosciences 1-800-628-7730 1-314-991-6034 technical@gbiosciences.com A Geno Technology, Inc. (USA) brand name Classic Immunoprecipitation Utilizes Protein A/G Agarose for Antibody Binding (Cat.

More information

Protocol for Western Blotting

Protocol for Western Blotting Protocol for Western Blotting Materials Materials used on Day 3 Protease inhibitor stock: 1 μg/μl pepstatin A in DMSO 200 μm leupeptin in OG Buffer 200 mm PMSF: Freshly made. Ex) 34.8 mg PMSF in 1 ml isopropanol

More information

RNA Fragment DeepSeq Library Preparation Protocol

RNA Fragment DeepSeq Library Preparation Protocol RNA Fragment DeepSeq Library Preparation Protocol I) LIGATION Recommended input: RNA between 0.05-2 pmol; must have 3' OH 1. Thaw 10X T4 RNA Ligase Reaction Buffer, 50% PEG8000, 20 mm DTT, 7 um App Adaptor

More information

How To Use An Enzymatics Spark Dna Sample Prep Kit For Ion Torrent

How To Use An Enzymatics Spark Dna Sample Prep Kit For Ion Torrent SPARK DNA Sample Prep Kit Ion Torrent (SPK0002-V08) Frequently Asked Questions Under what circumstances would I use SPARK DNA Sample Prep Kit for Ion Torrent? Enzymatics SPARK DNA Sample Prep Kit for Ion

More information

5PCR, real-time PCR, reverse transcription, and cloning

5PCR, real-time PCR, reverse transcription, and cloning 5PCR, real-time PCR, reverse transcription, and cloning 166 www.qiagen.com QIAGEN Product Guide 2007 PCR, real-time PCR, reverse transcription, and cloning 5 5.1 PCR and RT-PCR www.qiagen.com/pg/pcr Selection

More information

STA DARD OPERATI G PROCEDURE FOR THE DETECTIO OF AFRICA SWI E FEVER VIRUS (ASFV) BY REAL-TIME POLYMERASE CHAI REACTIO (PCR)

STA DARD OPERATI G PROCEDURE FOR THE DETECTIO OF AFRICA SWI E FEVER VIRUS (ASFV) BY REAL-TIME POLYMERASE CHAI REACTIO (PCR) STA DARD OPERATI G PROCEDURE FOR THE DETECTIO OF AFRICA SWI E FEVER VIRUS (ASFV) BY REAL-TIME POLYMERASE CHAI REACTIO (PCR) jmvizcaino@vet.ucm.es Av/ Puerta de Hierro s/n. 28040 Madrid. Tel: (34) 913944082

More information

Electrophoresis, cleaning up on spin-columns, labeling of PCR products and preparation extended products for sequencing

Electrophoresis, cleaning up on spin-columns, labeling of PCR products and preparation extended products for sequencing Electrophoresis, cleaning up on spin-columns, labeling of PCR products and preparation extended products for sequencing PAGE electrophoresis Polyacrylamide gel electrophoresis (PAGE) is used for separating

More information

VLLM0421c Medical Microbiology I, practical sessions. Protocol to topic J10

VLLM0421c Medical Microbiology I, practical sessions. Protocol to topic J10 Topic J10+11: Molecular-biological methods + Clinical virology I (hepatitis A, B & C, HIV) To study: PCR, ELISA, your own notes from serology reactions Task J10/1: DNA isolation of the etiological agent

More information

HighPure Maxi Plasmid Kit

HighPure Maxi Plasmid Kit HighPure Maxi Plasmid Kit For purification of high pure plasmid DNA with high yields www.tiangen.com PP120109 HighPure Maxi Plasmid Kit Kit Contents Storage Cat.no. DP116 Contents RNaseA (100 mg/ml) Buffer

More information

Real-Time PCR Vs. Traditional PCR

Real-Time PCR Vs. Traditional PCR Real-Time PCR Vs. Traditional PCR Description This tutorial will discuss the evolution of traditional PCR methods towards the use of Real-Time chemistry and instrumentation for accurate quantitation. Objectives

More information

Hepatitis B Virus Genemer Mix

Hepatitis B Virus Genemer Mix Product Manual Hepatitis B Virus Genemer Mix Primer Pair for amplification of HBV Specific DNA Fragment Includes Internal Negative Control Primers and Template Catalog No.: 60-2007-12 Store at 20 o C For

More information

Megaplex Pools For microrna Expression Analysis

Megaplex Pools For microrna Expression Analysis Megaplex Pools For microrna Expression Analysis Protocol Copyright 2008, 2010 Applied Biosystems. All rights reserved. For Research Use Only. Not for use in diagnostic procedures. Information in this document

More information

EZ-Run Protein Gel Solution. EZ-Run Protein Standards. EZ-Run Gel Staining Solution. Traditional SDS-Page Reagents. Protein Electrophoresis

EZ-Run Protein Gel Solution. EZ-Run Protein Standards. EZ-Run Gel Staining Solution. Traditional SDS-Page Reagents. Protein Electrophoresis EZ-Run Protein Gel Solution EZ-Run Protein Standards EZ-Run Gel Staining Solution Traditional SDS-Page Reagents Protein Electrophoresis protein electrophoresis Introduction Sodium dodecyl sulfate polyacrylamide

More information

Wizard DNA Clean-Up System INSTRUCTIONS FOR USE OF PRODUCT A7280.

Wizard DNA Clean-Up System INSTRUCTIONS FOR USE OF PRODUCT A7280. Technical Bulletin Wizard DNA Clean-Up System INSTRUCTIONS FOR USE OF PRODUCT A7280. PRINTED IN USA. Revised 4/06 AF9TB141 0406TB141 Wizard DNA Clean-Up System All technical literature is available on

More information

Aurora Forensic Sample Clean-up Protocol

Aurora Forensic Sample Clean-up Protocol Aurora Forensic Sample Clean-up Protocol 106-0008-BA-D 2015 Boreal Genomics, Inc. All rights reserved. All trademarks are property of their owners. http://www.borealgenomics.com support@borealgenomics.com

More information

RNA Extraction and Quantification, Reverse Transcription, and Real-time PCR (q-pcr)

RNA Extraction and Quantification, Reverse Transcription, and Real-time PCR (q-pcr) RNA Extraction and Quantification, Reverse Transcription, and Real-time Preparation of Samples Cells: o Remove media and wash cells 2X with cold PBS. (2 ml for 6 well plate or 3 ml for 6cm plate) Keep

More information

Quantitative Real Time PCR Protocol. Stack Lab

Quantitative Real Time PCR Protocol. Stack Lab Quantitative Real Time PCR Protocol Stack Lab Overview Real-time quantitative polymerase chain reaction (qpcr) differs from regular PCR by including in the reaction fluorescent reporter molecules that

More information

360 Master Mix. , and a supplementary 360 GC Enhancer.

360 Master Mix. , and a supplementary 360 GC Enhancer. Product Bulletin AmpliTaq Gold 360 Master Mix and 360 DNA Polymerase AmpliTaq Gold 360 Master Mix AmpliTaq Gold 360 DNA Polymerase 360 Coverage for a Full Range of Targets AmpliTaq Gold 360 Master Mix

More information

Catalogue. Catalogue. www. genemarkbio.com

Catalogue. Catalogue. www. genemarkbio.com Catalogue Catalogue 2 0 1 4 www. genemarkbio.com Company Profile Company Profile GMbiolab Co., Ltd. was founded in 2001 in Taiwan. We provide a variety of molecular biology kits and reagents, including

More information

Mir-X mirna First-Strand Synthesis and SYBR qrt-pcr

Mir-X mirna First-Strand Synthesis and SYBR qrt-pcr User Manual Mir-X mirna First-Strand Synthesis and SYBR qrt-pcr User Manual United States/Canada 800.662.2566 Asia Pacific +1.650.919.7300 Europe +33.(0)1.3904.6880 Japan +81.(0)77.543.6116 Clontech Laboratories,

More information