Real-Time Quantitative PCR Instruments and Consumables

Transcription

1 Index Page Sequence Detection Systems 2 Sequence Detection Systems Accessories and Softwares 3 Sequence Detection PCR Reagent Kits 4 Sequence Detection PCR Reagents 9 Sequence Detection Systems Disposables 13 TaqMan Food and Environmental Kits 15 Applied Biosystems 7900HT Micro Fluidic Cards 17 Sequence Detection Preformatted Assays 19 Training 21 Ordering Information 21 Trademarks 27 europe.appliedbiosystems.com Applera Italia Fillale della Applera Europe B.V. Page 1 of 27

2 Sequence Detection Systems ABI PRISM 7900HT Sequence Detection Each ,00 System with 384-Well Block Includes: Standard hardware configuration (peltier based thermal cycler, 384-well block, argon ion laser, CCD camera), Windows 2000 OS based PC, 17 flat panel monitor, Sequence Detection Software, PRIMER EXPRESS Oligonucleotide design software, User s Manual, Installation and Training ABI PRISM 7900HT Sequence Detection Each ,00 System with 384-Well Block and Automation Accessory Includes: Standard hardware configuration (peltier based thermal cycler, 384-well block, argon ion laser, CCD camera), Automation Accessory (five plate stacking positions, input up to 4 x well plates), Windows 2000 OS based PC, 17 flat panel monitor, Sequence Detection Software, PRIMER EXPRESS Oligonucleotide design software, User s Manual, Installation and Training ABI PRISM 7900HT Sequence Detection Each ,00 System 96 Well V Includes: Standard hardware configuration (peltier based thermal cycler, 96-well block, argon ion laser, CCD camera), Windows 2000 OS based PC, 17 flat panel monitor, Sequence Detection Software, PRIMER EXPRESS Oligonucleotide design software, User s Manual, Installation and Training ABI PRISM 7900HT Sequence Detection Each ,00 System 96 Well V and Automation Accessory Includes: Standard hardware configuration (peltier based thermal cycler, 96-well block, argon ion laser, CCD camera), Automation Accessory (five plate stacking positions, input up to 4 x well plates), Windows 2000 OS based PC, 17 flat panel monitor, Sequence Detection Software, PRIMER EXPRESS Oligonucleotide design software, User s Manual, Installation and Training. europe.appliedbiosystems.com Applera Italia Fillale della Applera Europe B.V. Page 2 of 27

3 ABI PRISM 7000 Sequence Detection System Each ,00 with Notebook Computer Includes: Notebook Computer with Windows 2000 OS, Sequence Detection Software, PRIMER EXPRESS Oligonucleotide design software, Users Manual, Installation and Training ABI PRISM 7000 Sequence Detection System Each ,00 with PC Tower Includes: Desktop Computer with Widows 2000 OS, 17" flat panel monitor, Sequence Detection Software, PRIMER EXPRESS Oligonucleotide design software, Users Manual, Installation and Training. Sequence Detection Systems Accessories and Softwares Well Block Upgrade for ABI PRISM Each 6.300, HT Sequence Detection System Well Block Upgrade for ABI PRISM Each 6.300, HT Sequence Detection System Automation Accessory Upgrade for ABI Each ,00 PRISM 7900HT Sequence Detection System Applied Biosystems 7900HT Micro Fluidic Card Each ,00 Upgrade Kit For the ABI PRISM 7900HT Sequence Detection System. Includes: 384-well Microcard cycling block, Centrifuge Buckets and Clips, Card Sealer and User s Manual, Service Installation SDS Software 2.1 for the 7900HT System, each ,00 Enterprise Edition Upgrade Kit Includes: Enterprise Software for high-throuhghput workflow and data management; Oracle data base, 5 client licenses, dedicated server computer with Windows 2000 OS and 17 flat panel monitor, router and cables, User s Manual, Service Installation RQ Manager Software for the SDS 7900HT System Each 3.770,00 Extension of the SDS 7900HT Enterprise Edition Software for completely automated relative gene expression analysis of up to 200 x 384-well plates in one analysis. Includes Software and User s Manual. europe.appliedbiosystems.com Applera Italia Fillale della Applera Europe B.V. Page 3 of 27

4 SNP Manager Software for the SDS 7900HT System Each 3.770,00 Extension of SDS 7900HT Enterprise Edition Software for completely automated SNP scoring analysis of up to 5000 x 384-well plates in one analysis. Includes Software and User s Manual. SSV00001 Celera Discovery System TM Database Each 9.500,00 Subscription One year personalized access for one user to the online research environment integrating public and proprietary genomic and biological information for human and mouse.academic, governmental and non-profit accounts are eligible for a special discount. Sequence Detection PCR Reagent Kits Unless noted otherwise, one reaction is defined as 50µl. Sequence Detection PCR Reagent Kits (without Controls) C TaqMan Universal PCR Master Mix, No 200 Rxn 470,00 AmpErase UNG Supplied at a 2x concentration. The mix is optimized for TaqMan reagent-based reactions and contains AmpliTaq Gold DNA Polymerase, dntps with dutp, Passive Reference 1, and optimized buffer components. 1 x 5ml vial. The protocol (P/N ) can be downloaded from the internet as pdf file: C TaqMan Universal PCR Master Mix 200 Rxn 525,00 Supplied at a 2x concentration. The mix is optimized for TaqMan reagent-based reactions and contains AmpliTaq Gold DNA Polymerase, AmpErase UNG, dntps with dutp, Passive Reference 1, and optimized buffer components. 1 x 5ml vial. The protocol (P/N ) can be downloaded from the internet as pdf file: europe.appliedbiosystems.com Applera Italia Fillale della Applera Europe B.V. Page 4 of 27

5 C TaqMan Universal PCR Master Mix, No AmpErase UNG, 2000 Rxn 4.180, Reactions Supplied at a 2x concentration. The mix is optimized for TaqMan reagent-based reactions and contains AmpliTaq Gold DNA Polymerase, dntps with dutp, Passive Reference 1, and optimized buffer components. 10 x 5ml vials in one box. The protocol (P/N ) can be downloaded from the internet as pdf file: C TaqMan Universal PCR Master Mix, 2000 Reactions 2000 Rxn 4.440,00 Supplied at a 2 x concentration. The mix is optimized for TaqMan reagent-based reactions and contains AmpliTaq Gold DNA Polymerase, AmpErase UNG, dntps with dutp, Passive Reference 1, and optimized buffer components. 10 x 5ml vials in one box. The protocol (P/N ) can be downloaded from the internet as pdf file: C TaqMan Universal PCR Master Mix, 50ml, No 2000 Rxn 4.040,00 AmpErase UNG Supplied at a 2x concentration. The mix is optimized for TaqMan reagent-based reactions and contains AmpliTaq Gold DNA Polymerase, dntps with dutp, Passive Reference 1, and optimized buffer components. 1 x 50ml vial. The protocol (P/N ) can be downloaded from the internet as pdf file: C TaqMan Universal PCR Master Mix, 50ml 2000 Rxn 4.310,00 Supplied at a 2 x concentration. The mix is optimized for TaqMan reagent-based reactions and contains AmpliTaq Gold DNA Polymerase, AmpErase UNG, dntps with dutp, Passive Reference 1, and optimized buffer components. 1x 50ml vial. The protocol (P/N ) can be downloaded from the internet as pdf file: C Pack, TaqMan Universal PCR Master Mix 2000 Rxn 4.580,00 Includes: 10 boxes of P/N The protocol (P/N ) can be downloaded from the internet as pdf file: europe.appliedbiosystems.com Applera Italia Fillale della Applera Europe B.V. Page 5 of 27

6 C6 N TaqMan PCR Core Reagent Kit 200 Rxn 505,00 Includes: 250 Units AmpliTaq Gold DNA Polymerase; 100 Units AmpErase UNG; dutp, datp, dctp, dgtp; 1.2ml 10 x TaqMan Buffer A; 2 x 1.5ml vials of 25mM MgCl 2 Solution. The protocol (P/N ) can be downloaded from the internet as pdf file: C Pack, TaqMan PCR Reagent Kit 2000 Rxn 4.650,00 Includes: 10 x N TaqMan PCR Core Reagent Kit. The protocol (P/N ) can be downloaded from the internet as pdf file: C TaqMan 1000 Reaction PCR Core Reagent Kit 1000 Rxn 2.140,00 Includes: 1250 Units AmpliTaq Gold DNA Polymerase; 500 Units AmpErase UNG; dutp, datp, dctp, dgtp, 10 x TaqMan Buffer A,15ml 25mM MgCl 2 Solution. The protocol (P/N ) can be downloaded from the internet as pdf file: C SYBR Green PCR Master Mix 40 Rxn 121,00 Includes: 1 x 1ml vial. The protocol (P/N ) can be downloaded from the internet as pdf file: C SYBR Green PCR Master Mix 200 Rxn 445,00 Supplied at a 2x concentration. The mix is optimized for SYBR Green dye reactions and contains SYBR Green 1 dye, AmpliTaq Gold DNA Polymerase, dntps with dutp, Passive Reference 1, and optimized buffer components. The protocol (P/N ) can be downloaded from the internet as pdf file: C Pack, SYBR Green PCR Master Mix 2000 Rxn 4.040,00 Includes: 10 x The protocol (P/N ) can be downloaded from the internet as pdf file: C SYBR Green PCR Master Mix, 50 ml 2000 Rxn 3.990,00 Includes: 1 x 50ml vial. The protocol (P/N ) can be downloaded from the internet as pdf file: europe.appliedbiosystems.com Applera Italia Fillale della Applera Europe B.V. Page 6 of 27

7 C SYBR Green PCR Core Reagents Kit 200 Rxn 505,00 Includes: 250 Units AmpliTaq Gold DNA Polymerase, 100 Units AmpErase UNG, dntp Mix with dutp, 10 x SYBR Green PCR Buffer, 25mM MgCl 2 Solution. The protocol (P/N ) can be downloaded from the internet as pdf file: C Pack, SYBR Green PCR Core Reagents 2000 Rxn 4.580,00 Includes: 10 x The protocol (P/N ) can be downloaded from the internet as pdf file: Sequence Detection RT-PCR Reagent Kits (without Controls) C TaqMan One-Step RT-PCR Master Mix 200 Rxn 740,00 Reagents Kit Contains two components. Vial 1 (5.0ml): AmpliTaq Gold DNA Polymerase mix (2x concentration) is optimized for TaqMan reagent-based reactions and contains AmpliTaq Gold DNA Polymerase, dntps with dutp, Passive Reference 1, and optimized buffer components. Vial 2 (250µl): RT enzyme mix (40x concentration) contains MultiScribe TM Reverse Transcriptase and RNase Inhibitor. The protocol (P/N ) can be downloaded from the internet as pdf file: C Pack, TaqMan One-Step RT-PCR Master Mix 2000 Rxn 6.800,00 Reagents Kit Includes: 10 x P/N The protocol (P/N ) can be downloaded from the internet as pdf file: C6 N TaqMan Gold RT-PCR Reagents without 200 Rxn 945,00 controls Includes: TaqMan Reverse Transcription Reagents (P/N N ) and TaqMan PCR Core Reagent Kit (P/N N ) and Protocol (P/N ). The protocol can also be downloaded from the internet as pdf file: europe.appliedbiosystems.com Applera Italia Fillale della Applera Europe B.V. Page 7 of 27

8 C Pack, TaqMan Gold RT-PCR Reagents without 2000 Rxn 8.650,00 controls Includes: 10 x N , 1 x Protocol P/N The protocol can also be downloaded from the internet as pdf file: C6 N TaqMan Reverse Transcription Reagents 200 Rxn 440,00 Includes: MultiScribe Reverse Transcriptase (5000 Units), RNase Inhibitor (2 tubes), dntp Mixture, Oligo d(t) 16, Random Hexamers, 10x RT buffer, MgCl 2 solution. The protocol (P/N ) can be downloaded from the internet as pdf file: C Pack, TaqMan Reverse Transcription Reagents 2000 Rxn 4.030,00 Includes: 10 x P/N N , 1 x Protocol P/N The protocol can also be downloaded from the internet as pdf file: C6 N TaqMan EZ RT-PCR Core Reagents 200 Rxn 945,00 Includes: 1000 Units rtth DNA Polymerase, 100 Units AmpErase UNG, dutp, datp, dctp, dgtp, 5 x TaqMan EZ Buffer, 25 mm Mn(OAc) 2. The protocol (P/N ) can be downloaded from the internet as pdf file: C Pack, TaqMan EZ RT-PCR Reagent Kit 2000 Rxn 8.650,00 Includes: 10 x P/N N , 1 x Protocol P/N The protocol can also be downloaded from the internet as pdf file: C SYBR Green RT-PCR Reagents 200 Rxn 880,00 Includes: SYBR Green PCR Master Mix (P/N ), TaqMan Reverse Transcriptase Reagents (P/N N ). The protocol (P/N ) can be downloaded from the internet as pdf file: C High Capacity cdna Archive Kit 200 Rxn 740,00 Includes: Random Primers, optimized RT Buffer, dntps and MultiScribe TM MuLV Reverse Transcriptase for the quantitative conversion of up to 10µg of total RNA in a single 100µl reaction to single stranded cdna. The protocol (P/N ) can be downloaded from the internet as pdf file: europe.appliedbiosystems.com Applera Italia Fillale della Applera Europe B.V. Page 8 of 27

9 Sequence Detection Reagent Kits (with Controls) C6 N TaqMan PCR Reagent Kit 200 Rxn 675,00 Includes: TaqMan Core Reagent Kit (P/N N ), TaqMan beta-actin Control Reactions (P/N ) and Protocol (P/N ). The protocol can also be downloaded from the internet as pdf file: C6 N TaqMan Gold RT-PCR Reagents with controls 200 Rxn 1.210,00 Includes: TaqMan PCR Core Reagent Kit (N ), TaqMan Reverse Transcriptase Reagents (P/N N ), and TaqMan GAPDH Control Reagents (P/N ), Protocol (P/N ), for 10µl Reverse Transcription Rxn and 50µl PCR Rxn. The protocol can also be downloaded from the internet as pdf file: C6 N TaqMan EZ RT-PCR Kit 200 Rxn 1.210,00 Includes: 1000 Units rtth DNA Polymerase, 100 Units AmpErase UNG, dutp, datp, dctp, dgtp, 5 x TaqMan EZ Buffer, 25mM Mn(OAc) 2, 100 GAPDH control reactions, and Protocol P/N The protocol can also be downloaded from the internet as pdf file: Sequence Detection PCR Reagents Sequence Detection Control Reagents C TaqMan RNase P Instrument Verification Each 675,00 Plate 96-well optical plate, preloaded with 96 x 50 µl TaqMan reagent-based reactions, including primers and probe (FAM TM dye), sealed with optical caps. Different template concentrations for standard curve and verification of 2-fold discrimination on ABI PRISM Sequence Detection Systems 7700, 7000, and 7900HT with 96-well block. europe.appliedbiosystems.com Applera Italia Fillale della Applera Europe B.V. Page 9 of 27

10 C TaqMan RNase P 384-well Instrument Each 875,00 Verification Plate 384-well optical plate, preloaded with 384 x 20µl TaqMan reagent-based reactions, including primers and probe (FAM TM dye), sealed with optical adhesive cover. Different template concentrations for standard curve and verification of 2-fold discrimination on ABI PRISM 7900HT Sequence Detection System. C TaqMan Human RNase P Detection Reagent Kit 100 Rxn 236,00 Includes: 20 x RNase P Mix with Forward and Reverse Primers, RNase P Probe (FAM TM dye), Human Genomic Control DNA (10 ng/µl). C TaqMan Human RNase P Control Reagents Kit 1000 Rxn 605,00 Includes: 20 x RNase P Mix with Forward and Reverse Primers, primer limited, and RNase P probe (VIC dye), 2.5ml; Human Genomic Control DNA (10 ng/µl, 100 µl); Product Insert with recommended procedure. For use as endogenous control in multiplex reactions. C TaqMan Ribosomal RNA Control Reagents 1000 Rxn 675,00 (VIC dye) Includes: Human Control RNA, rrna Probe (VIC dye) rrna Forward Primer, and rrna Reverse Primer. The protocol (P/N ) can be downloaded from the internet as pdf file: C TaqMan Rodent GAPDH Control Reagents 1000 Rxn 675,00 (VIC dye) Includes: Rodent Control RNA, rodent GAPDH Probe (VIC dye), rodent GAPDH Forward and Reverse Primer. The protocol (P/N ) can be downloaded from the internet as pdf file: C TaqMan Human GAPDH Control Reagents 100 Rxn 305,00 (JOE TM dye) Includes: Human Control RNA, GAPDH Probe (JOE TM dye), Forward and Reverse Primer. C TaqMan b-actin Detection Reagent 100 Rxn 305,00 Includes: beta-actin Forward and Reverse Primer (500µl, 3mM), beta-actin Probe (500µl, 2µM probe, FAM TM dye), 100µl 10ng/µl human genomic DNA. europe.appliedbiosystems.com Applera Italia Fillale della Applera Europe B.V. Page 10 of 27

11 C TaqMan DNA Template Reagents 1 plate 385,00 Includes: 1 tube with beta-actin probe(fam TM ), Forward and Reverse Primer, 6 tubes with different concentrations of human Raji DNA, unknown DNA control. C TaqMan Exogenous Internal Positive 200 Rxn 270,00 Control Reagents (VIC dye) Includes: 10x exogenous IPC Primer and Probe (VIC dye) Mix, 10x exogenous IPC, blocking reagent, and 50x exogenous IPC target. Kit represents an IPC assay (optimized with TaqMan Universal PCR Master Mix) to be used with custom +/- assays. The protocol (P/N ) can be downloaded from the internet as pdf file: C Pack, TaqMan Exogenous Internal 1000 Rxn 1.210,00 Positive Control Reagents (VIC dye) Includes: 5 x P/N C TaqMan Exogenous Internal Positive 200 Rxn 775,00 Control Reagents with TaqMan Universal PCR Master Mix Includes: P/N and P/N The protocol (P/N ) can be downloaded from the internet as pdf file: Sequence Detection Reagent Components C TaqMan 1000 Reactions Gold with Buffer A 1000 Rxn 1.350,00 Includes: 1250 Units AmpliTaq Gold DNA Polymerase, 6ml 10x TaqMan Buffer A, 15ml 25mM MgCl 2. The protocol (P/N ) can be downl from the internet as pdf file: C MultiScribe TM Reverse Transcriptase 5000 Units 81,00 Recombinant Moloney Murine Leukaemia Virus Reverse Transcriptase for 100 x 20 µl reactions. C Sequence Detection Systems Spectral Calibration Each 540,00 Kit Includes dye standards for: FAM TM, JOE TM, ROX TM, SYBR Green, TAMRA TM, TET TM and VIC dyes. europe.appliedbiosystems.com Applera Italia Fillale della Applera Europe B.V. Page 11 of 27

12 C Sequence Detection Systems 384-well Spectral 2/Pkg 540,00 Calibration Kit One 384-Well Optical Background Plate, preloaded and sealed, and one 384-well Spectral Calibration Plate, preloaded and sealed, with 8 dye standards loaded in alphabetical order: FAM TM, JOE TM, NED TM, ROX TM, SYBR Green, TAMRA TM, TET TM and VIC dyes. For use on ABI PRISM 7900HT instrument. Each dye is contained in 3 columns. 20µl per well. C ABI PRISM 7900HT SDS Spectral Dye 3/Pkg 540,00 Calibration Kit, 96-Well Includes: Three ABI PRISM TM Optical 96-Well Reaction Plates: one preloaded and sealed Background plate, and two preloaded and sealed Spectral Calibration plates containing eight separate dye standards (FAM TM, JOE TM, NED TM, ROX TM, SYBR Green, TAMRA TM, TET TM, and VIC dye). C ABI PRISM 7000 SDS Spectral Dye 8/Pkg 1.010,00 Calibration Kit Includes: one 96-Well Background plate and seven 96-well Spectral Calibration Plates, preloaded with dye standard and sealed. One dye per calibration plate: FAM TM, JOE TM, NED TM, ROX TM, SYBR Green, TAMRA TM and VIC dye. C3 N RNase Inhibitor, 20 Units/µl, 100 reactions 2000 Units 81,00 Recombinant protein for 100 x 20µl reactions. C3 N GeneAmp dntp Blend with dttp 1ml 67,00 Single vial blend, contains a 10mM solution of dntp (2.5mM each of datp, dctp, dgtp and dttp) for 200 x 50µl reactions. C3 N GeneAmp dntp Blend, 12.5mM with dutp 1ml 101,00 Single vial blend, contains each datp, dctp, dgtp at 2.5mM and dutp at 5mM for 200 x 50µl reactions. C3 N Oligo d(t) 16 Primers, 100µl, 50µM 100 Rxn 67,00 C3 N Random Hexamers, 100µl, 50µM. 100 Rxn 67,00 C TaqMan Control Total RNA (Human) 50ng/µl 100 µl 67,00 C TaqMan Control Genomic DNA (Human) 10ng/µl 2 x 100 µl 67,00 C % Glycerol Solution, Molecular Biology Grade 100 ml 67,00 C2 N AmpErase Uracil N-glycosylase (UNG), 100 Units 168,00 1 Unit / µl europe.appliedbiosystems.com Applera Italia Fillale della Applera Europe B.V. Page 12 of 27

13 Sequence Detection Systems Disposables C MicroAmp Optical 96-well Reaction Plate, 20/Pkg 148,00 with Barcode (code 128) C MicroAmp Optical 96-well Reaction Plate 500/Pkg 3.230,00 with Barcode (code 128), 25-Pack Includes: 25 x P/N C MicroAmp Optical 96-Well Reaction Plate 20 plates/pkg 255,00 and ABI PRISM TM Flat Optical Caps Includes: 20 Plates (P/N ) with barcode (code 128) and 2400 Flat Optical Caps ( ). C ABI PRISM TM Optical Adhesive Covers and 100/Pkg 875,00 96-Well Optical Reaction Plates Includes: 1 x P/N (ABI PRISM Optical Adhesive Covers) and 5 x P/N (ABI PRISM Optical 96-Well Reaction Plates) First time users should read User Bulletin 117UB01, downloadable from the internet as pdf file: C ABI PRISM 384-well Clear Optical Reaction Plate 50/Pkg 310,00 with Barcode (code 128) C ABI PRISM 384-Well Clear Optical Reaction Plate 500/Pkg 2.620,00 with Barcode (code 128) Includes: 10 x P/N C ABI PRISM TM 384-Well Clear Reaction Plate 1000/Pkg 4.040,00 with Barcode (code 128), 1000-Pack C ABI PRISM TM 384-Well Clear Reaction Plate 1000/Pkg 3.910,00 without Barcode, 1000-Pack C ABI PRISM TM 384-Well Clear Optical 50/Pkg 350,00 Reaction Plate with 3 Barcodes (code 128) Barcode labels are on both long sides and the right hand short side of the plate. Allows processing of plates by several robotic plate handling devices. C ABI PRISM TM 384-Well Clear Optical 500/Pkg 2.630,00 Reaction Plate with 3 Barcodes (code 128), 10-pack Barcode labels are on both long sides and the right hand short side of the plate. Allows processing of plates by several robotic plate handling devices. europe.appliedbiosystems.com Applera Italia Fillale della Applera Europe B.V. Page 13 of 27

14 C ABI PRISM TM Optical Adhesive Cover Starter 20/Pkg 67,00 Kit Includes: 20 ABI PRISM Optical Adhesive Covers, 1 x ABI PRISM Optical Cover Compression Pad, 1 x Applicator. First time users should read User Bulletin 117UB01, downloadable from the internet as pdf file: C ABI PRISM TM Optical Adhesive Covers 100/Pkg 215,00 Includes: 100 x ABI PRISM Optical Adhesive Covers. First time users should read User Bulletin 117UB01, downloadable from the internet as pdf file: C Adhesive Seal Applicator Kit 5/Pkg 74,00 Spare applicators for sealing Optical Adhesive Covers on reaction plates. C ABI PRISM TM Optical Cover Compression Pads 5/Pkg 59,00 C ABI PRISM TM 96-Well Snap-on Compression 9/Pkg 405,00 Pad Kit For use with 96-well plates in the plate stacking positions of the ABI PRISM 7900 Sequence Detection System Automation Acessory. C ABI PRISM TM Optical Caps, 8 Flat Caps/Strip 2400 caps/pkg 114,00 Compatible with ABI PRISM 7900HT, 7700, 7000, and GeneAmp 5700 Sequence Detection Systems. 300 strips. C ABI PRISM TM Optical Tubes, 8 Tubes/Strip 1000 tubes/pkg 108,00 For use on the ABI PRISM 7000, 7700 and GeneAmp 5700 Sequence Detection systems only. C5 N MicroAmp Optical Tubes 2000/Pkg 270,00 For use on the ABI PRISM 7000, 7700 and GeneAmp 5700 Sequence Detection systems only. C MicroAmp Tray/Retainer Set for 96 Tubes 10/Pkg 69,00 For use with single or strip optical tubes on the ABI PRISM 7000, 7700 and GeneAmp 5700 Sequence Detection Systems, and 310 Autosampler. C MicroAmp Multi Removal Tool Each 21,00 Multi-functional tool. Used for removing Microplates from a Thermal Cycler sample block; removing strip caps from Microplates and Tray/Retainer assemblies; and opening MicroAmp or GeneAmp tubes. europe.appliedbiosystems.com Applera Italia Fillale della Applera Europe B.V. Page 14 of 27

15 ABI PRISM TM Cap Installing Tool Each 34,00 Used for installing and removing ABI PRISM Flat Optical Caps from the MicroAmp plate or ABI PRISM 96-Well Optical Plates or Tray/Retainer Assemblies. C Splash Free Support Base 10/Pkg 83,00 For use on GeneAmp 9600, 9700 thermal cyclers and ABI PRISM 7700 Sequence Detection System; needs retainer to hold 0.2 ml tubes. Replaces MicroAmp Base N TaqMan Food and Environmental Kits TaqMan GMO Detection Kits For ABI PRISM 7700, 7900HT and 7000 Sequence Detection Systems. A TaqMan GMO Soy 35S Detection Kit, with 100 Rxn 1.000,00 User Guide Includes: AmpliTaq Gold DNA Polymerase, Reaction Mix with primers and TAMRA TM dye-quenched probes with FAM TM and VIC dye label. Positive and Negative Controls, certain specific PCR Service Rights, Quick Reference Card and User Guide. The User Guide (P/N ) and Quick Reference Card (P/N ) can be downloaded free of charge from the internet as pdf file: A TaqMan GMO Soy 35S Detection Kit 100 Rxn 1.000,00 Includes: AmpliTaq Gold DNA Polymerase, Reaction Mix with primers and TAMRA TM dye-quenched probes with FAM TM and VIC dye label. Positive and Negative Controls, certain specific PCR Service Rights. The User Guide (P/N ) and Quick Reference Card (P/N ) can be downloaded free of charge from the internet as pdf file: europe.appliedbiosystems.com Applera Italia Fillale della Applera Europe B.V. Page 15 of 27

16 A TaqMan GMO Maize 35S Detection Kit, with 100 Rxn 1.000,00 User Guide Includes: AmpliTaq Gold DNA Polymerase, Reaction Mix with primers and TAMRA TM dye-quenched probes with FAM TM and VIC dye label. Positive and Negative Controls, certain specific PCR Service Rights, Quick Reference Card and User Guide. The User Guide (P/N ) and Quick Reference Card (P/N ) can be downloaded free of charge from the internet as pdf file: A TaqMan GMO Maize 35S Detection Kit 100 Rxn 1.000,00 Includes: AmpliTaq Gold DNA Polymerase, Reaction Mix with primers and TAMRA TM dye-quenched probes with FAM TM and VIC dye label. Positive and Negative Controls, certain specific PCR Service Rights. The User Guide (P/N ) and Quick Reference Card (P/N ) can be downloaded free of charge from the internet as pdf file: A TaqMan GMO Quick Refence Card Each 31,00 Also downloadable free of charge from the internet as pdf file: A TaqMan GMO User Guide Each 31,00 Also downloadable free of charge from the internet as pdf file: Pathogen Detection Reagents A TaqMan E. coli O157:H7 Detection Kit 100 Tests 875,00 Not recommended for use on the ABI PRISM 7000 Sequence Detection System. Includes: TaqMan E. coli O175 : H7 Reaction Mix with TET TM dye-labelled probe, AmpliTaq Gold (5U/µl, 100µl), positive and negative controls, certain specific PCR Service Rights. The User Guide (P/N ) and Quick Reference Card (P/N ) can be downloaded free of charge from the internet as pdf file: A TaqMan Pathogen Detection User Guide Each 34,00 also downloadable free of charge from the internet as pdf file: europe.appliedbiosystems.com Applera Italia Fillale della Applera Europe B.V. Page 16 of 27

17 A TaqMan Pathogen Detection Quick Reference Each 34,00 Card Also downloadable free of charge from the internet as pdf file: A PrepMan Ultra TM Sample Preparation Reagent 100 Rxn 133,00 with Protocol, 20 ml Homogeneous ready-to-use solution designed for the preparation of DNA template. For bacterial and fungal templates, DNA preparations for GMO testing and other cells. 20ml vial includes Quick Start Guide and Protocol P/N The protocol can also be downloaded from the internet as PDF file at: A PrepMan TM Ultra Sample Preparation 100 Rxn 133,00 Reagent, 20ml Homogeneous ready-to-use solution designed for the preparation of DNA template. For bacterial and fungal templates, DNA preparations for GMO testing and other cells. 20ml vial. The protocol (P/N ) and Quick Start Card (P/N ) can be downloaded free of charge from the internet as pdf file: Applied Biosystems 7900HT Micro Fluidic Cards Delivery time: 5-6 weeks. Applied Biosystems 7900HT Micro Fluidic Card for Gene Expression HT Micro Fluidic Card for Gene Expression; 1 card 340,00 Configuration 1 11 unique assays, 1 mandatory control, 4 replicates per biological sample, 8 unique port sets. Minimum order volume: 10 cards. Micro Fluidic Cards can be custom-configured and ordered at HT Micro Fluidic Card for Gene Expression; 1 card 365,00 Configuration 2 23 unique assays, 1 mandatory control, 2 replicates per biological sample, 8 unique port sets. Minimum order volume: 10 cards. Micro Fluidic Cards can be custom-configured and ordered at europe.appliedbiosystems.com Applera Italia Fillale della Applera Europe B.V. Page 17 of 27

18 HT Micro Fluidic Card for Gene Expression; 1 card 365,00 Configuration 3 23 unique assays, 1 mandatory control, 4 replicates per biological sample, 4 unique port sets. Minimum order volume: 10 cards. Micro Fluidic Cards can be custom-configured and ordered at HT Micro Fluidic Card for Gene Expression; 1 card 390,00 Configuration 4 47 unique assays, 1 mandatory control, 1 replicate per biological sample, 8 unique port sets. Minimum order volume: 10 cards. Micro Fluidic Cards can be custom-configured and ordered at HT Micro Fluidic Card for Gene Expression; 1 card 390,00 Configuration 5 47 unique assays, 1 mandatory control, 2 replicates per biological sample, 4 unique port sets. Minimum order volume: 10 cards. Micro Fluidic Cards can be custom-configured and ordered at HT Micro Fluidic Card for Gene Expression; 1 card 390,00 Configuration 6 47 unique assays, 1 mandatory control, 4 replicates per biological sample, 2 unique port sets. Minimum order volume: 10 cards. Micro Fluidic Cards can be custom-configured and ordered at HT Micro Fluidic Card for Gene Expression; 1 card 410,00 Configuration 7 95 unique assays, 1 mandatory control, 1 replicate per biological sample, 4 unique port sets. Minimum order volume: 10 cards. Micro Fluidic Cards can be custom-configured and ordered at HT Micro Fluidic Card for Gene Expression; 1 card 410,00 Configuration 8 95 unique assays, 1 mandatory control, 2 replicates per biological sample, 2 unique port sets. Minimum order volume: 10 cards. Micro Fluidic Cards can be custom-configured and ordered at europe.appliedbiosystems.com Applera Italia Fillale della Applera Europe B.V. Page 18 of 27

19 HT Micro Fluidic Card for Gene Expression; 1 card 410,00 Configuration 9 95 unique assays, 1 mandatory control, 4 replicates per biological sample, 1 unique port set. Minimum order volume: 10 cards. Micro Fluidic Cards can be custom-configured and ordered at HT Micro Fluidic Card for Gene Expression; 1 card 340,00 Configuration unique assays, 1 mandatory control, 1 replicate per biological sample, 1 unique port set. Minimum order volume: 50 cards. Micro Fluidic Cards can be custom-configured and ordered at Sequence Detection Preformatted Assays Applied Biosystems 7900HT Immune Profiling Micro 10/Pkg 2.280,00 Fluidic Card For use on ABI PRISM 7900HT Sequence Detection System with Micro Fluidic Card Upgrade. Includes 96 different assays in quadruplicate, incl. 6 Endogenous Control Assays. C TaqMan Cytokine Gene Expression Plate 1 2/Pkg 1.250,00 with TaqMan Universal PCR Master Mix and Control Total RNA Multiplex assay system for use on ABI PRISM 7700, 7900HT and 7000 Sequence Detection Systems. Includes: two MicroAmp Optical 96-well Reaction Plates pre-loaded with TaqMan primers and probes for 12 human cytokine targets (replicates of 8) and the 18S Ribosomal RNA endogenous control (in all 96 wells). TaqMan primer and probe concentrations are optimized for multiplex PCR at 50µl utilizing FAM TM and VIC dyes. Configuration includes TaqMan Universal PCR Master Mix, MicroAmp Optical Caps, Control Total RNA (Human), and Protocol. Targets:IL-1a, IL-1b, IL-2, IL-4, IL-5, IL-8, IL-10, IL-12p35, IL-12p40, IL-15, IFN gamma, TNF alpha. The protocol (P/N ) can be downloaded from the internet as pdf file: europe.appliedbiosystems.com Applera Italia Fillale della Applera Europe B.V. Page 19 of 27

20 C TaqMan Cytokine Gene Expression Plate 1 2/Pkg 1.190,00 with TaqMan Universal PCR Master Mix Multiplex assay system for use on ABI PRISM 7700, 7900HT and 7000 Sequence Detection Systems. Includes: two MicroAmp Optical 96-well Reaction Plates pre-loaded with TaqMan primers and probes for 12 human cytokine targets (replicates of 8) and the 18S Ribosomal RNA endogenous control (in all 96 wells). TaqMan primer and probe concentrations are optimized for multiplex PCR at 50 µl utilizing FAM TM and VIC dyes. Configuration includes TaqMan Universal PCR Master Mix, MicroAmp Optical Caps, Control Total RNA (Human), and Protocol. Targets:IL-1a, IL-1b, IL-2, IL-4, IL-5, IL-8, IL-10, IL-12p35, IL-12p40, IL-15, IFN gamma, TNF alpha. The protocol (P/N ) can be downloaded from the internet as pdf file: C TaqMan Human Endogenous Control Plate 2/Pkg 1.250,00 with TaqMan Universal PCR Master Mix and Control Total RNA Includes: two MicroAmp Optical 96-well Reaction Plates pre-loaded with TaqMan primers and probes for 11 human endogenous control targets and an internal positive control (IPC) in replicates of 8. TaqMan primer and probe concentrations are optimized for 50µl reactions and utilize VIC dye and TAMRA TM dye quencher. Configuration includes TaqMan Universal PCR Master Mix, MicroAmp Optical Caps, Control Total RNA (Human), and Protocol. Human Targets: 18S ribosomal RNA, acidic ribosomal protein (PO), beta-actin, beta-glucuronidase (hugus), beta-2-microglobulin (B2M), cyclophilin (CYC), glyceroaldehyde phosphate dehydrogenase (GAPDH), hypoxanthine ribosyltransferase (HPRT), Glycerokinase (PGK), Transcription factor IID/TATA binding factor (TFIID/TBP), transferrin receptor (TfR), Internal Positive Control (IPC). The protocol (P/N ) can be downloaded from the internet as pdf file: europe.appliedbiosystems.com Applera Italia Fillale della Applera Europe B.V. Page 20 of 27

21 C TaqMan Human Endogenous Control Plate 2/Pkg 1.190,00 with TaqMan Universal PCR Master Mix Includes: two MicroAmp Optical 96-well Reaction Plates pre-loaded with TaqMan primers and probes for 11 human endogenous control targets and an internal positive control (IPC) in replicates of 8. TaqMan primer and probe concentrations are optimized for 50µl reactions and utilize VIC dye and TAMRA TM dye quencher. Configuration includes TaqMan Universal PCR Master Mix and MicroAmp Optical Caps. Human Targets: 18S ribosomal RNA, acidic ribosomal protein (PO), beta-actin, beta-glucuronidase (hugus), beta-2-microglobulin (B2M), cyclophilin (CYC), glyceroaldehyde phosphate dehydrogenase (GAPDH), hypoxanthine ribosyltransferase (HPRT), Glycerokinase (PGK), Transcription factor IID/TATA binding factor (TFIID/TBP), transferrin receptor (TfR), Internal Positive Control. The protocol (P/N ) can be downloaded from the internet as pdf file: Training TRAIN-7700 ABI PRISM 7700 Sequence Detection System 1 pers / 2 days 975,00 User Training** TRAIN-7000 ABI PRISM 7000 Sequence Detection System 1 pers / 2 days 975,00 User Training** TRAIN-7900HT ABI PRISM 7900HT Sequence Detection 1 pers / 2 days 975,00 System User Training** The training courses cover the effective operation of the ABI PRISM Sequence Detection Systems. The courses include instrument operation, optimal use of the run set up and Analysis Software, assay optimisation, primer and probe design and troubleshooting. **Training fees do not include travel and subsistence. Ordering Information Delivery charge per order 15,00 europe.appliedbiosystems.com Applera Italia Fillale della Applera Europe B.V. Page 21 of 27

22 europe.appliedbiosystems.com Applera Italia Fillale della Applera Europe B.V. Page 22 of 27

23 For further information, to order or to obtain a quotation please contact : Applera Italia Via Tiepolo 18 I Monza (MI) Tel: Fax: Italy.Order@eur.appliedbiosystems.com Visit us at europe.appliedbiosystems.com and register at New Store Customer to get a complete overview of products, create your personal catalogue, see full pricing information, place orders and check your order status. PLEASE NOTE: Applied Biosystems markets products for specific uses. Please refer to the product label for the intended use statements of specific products. Applied Biosystems products are intended For Research Use Only. Not for use in diagnostic procedures. This price list supersedes all previous price lists and is subject to change without notice. This price list is only for distribution within Europe. All Prices Quoted Exclude V.A.T Applied Biosystems. All rights reserved. europe.appliedbiosystems.com Applera Italia Fillale della Applera Europe B.V. Page 23 of 27

24 Trademarks Applied Biosystems, MicroAmp, Primer Express, and VIC are registered trademarks and AB (Design), ABI PRISM, Applera, FAM, JOE, MultiScribe, NED, ROX, TAMRA, TET, and VIC are trademarks of Applera Corporation or its subsidiaries in the US and/or certain other countries. AmpErase, AmpliTaq Gold, GeneAmp, and TaqMan are registered trademarks of Roche Molecular Systems, Inc. SYBR is a registered trademark of Molecular Probes, Inc. All other trademarks are the sole property of their respective owners. PLEASE NOTE 1. Notice to Purchaser: Authorized Thermal Cylcer The Sequence Detection System Base Unit, in combination with its immediately attached sample block modules,comprise an instrument that is an Authorized Thermal Cycler. The purchase price of the 2700 PCR System or the 9700 PCR System Base Base Unit includes the up-front fee component of a license under United States Patent Nos. 4,683,195, 4,683,202 and 4,965,188, owned by Roche Molecular Systems, Inc., and under corresponding claims in patents outside the United States, owned by F. Hoffmann-La Roche Ltd, covering the Polymerase Chain Reaction ("PCR") process to practice the PCR process for internal research and development using this instrument. The running royalty component of that license may be purchased from Applied Biosystems or obtained by purchasing Authorized Reagents. This instrument is also an Authorized Thermal Cycler for use with applications licenses available from Applied Biosystems. Its use with Authorized Reagents also provides a limited PCR license in accordance with the label rights accompanying such reagents. Purchase of this product does not itself convey to the purchaser a complete license or right to perform the PCR process. NOTICE TO PURCHASER: DISCLAIMER OF LICENCE: Sequence Detection Systems: No rights for any application including any in vitro diagnostic application, are conveyed expressly, by implication or by estoppel under any patent or patent applications claiming homogeneous or real-time detection methods, including patents covering such methods used in conjunction with the PCR process or other amplification processes. The 5 nuclease detection assay and certain other homogeneous or real-time amplification and detection methods are covered by United States Patent Nos. 5,210,015, 5,487,972, 5,804,375 and 5,994,056, owned by Roche Molecular Systems, Inc.; by corresponding patents and patent applications outside the United States, owned by F. Hoffmann-La Roche Ltd; and by United States Patent Nos. 5,538,848 and 6,030,787, and corresponding patents and patent applications outside the United States, owned by Applera Corporation. Purchase of this instrument conveys no license or right under the foregoing patents. Use of these and other patented processes in conjunction with the PCR process requires a license. For information on obtaining licenses, contact the Director of Licensing at Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, or The Licensing Department, Roche Molecular Systems, Inc., 1145 Atlantic Avenue, Alameda, California, 94501, USA. europe.appliedbiosystems.com Applera Italia Fillale della Applera Europe B.V. Page 24 of 27

25 2. The combination of the ABI PRISM 7900HT Sequence Detection System 96-Well Block Upgrade Accessory and the 384-Well Block Upgrade Accessory Interchangeable Sample Block Modules with an ABI PRISM 7900HT Sequence Detection System Base Unit is licensed under the thermal cycler apparatus claims of U.S. Patent No. 5,333,675 and counterpart patent claims in other countries. 3. The combination of the ABI PRISM 7900HT Micro Fluidic Card Block Upgrade Interchangeable Sample Block Module with an ABI PRISM 7900HT Sequence Detection System Base Unit is licensed under the thermal cycler apparatus claims of U.S. Patent No. 5,333,675 and counterpart patent claims in other countries. 4. Notice to Purchaser: License Disclaimer: SDS Software Purchase of this software product alone does not imply any license under any process, instrument or other apparatus, system, composition, reagent or kit rights under patent claims owned or otherwise controlled by Applera Corporation, either expressly or by estoppel. 5. NOTICE TO PURCHASER: LIMITED LABEL LICENSE: AmpliTaq Gold A license under U.S. Patents 4,683,202, 4,683,195, and 4,965,188 or their foreign counterparts, owned by Roche Molecular Systems, Inc. and F. Hoffmann-La Roche Ltd (Roche), for use in research and development, has an up-front fee component and a running-royalty component. The purchase price of this product includes limited, non-transferable rights under the running-royalty component to use only this amount of the product to practice the Polymerase Chain Reaction (PCR) and related processes described in said patents solely for the research and development activities of the purchaser when this product is used in conjunction with a thermal cycler whose use is covered by the up- front fee component. Rights to the up-front fee component must be obtained by the end user in order to have a complete license. These rights under the up-front fee component may be purchased from Applied Biosystems or obtained by purchasing an authorized thermal cycler. No right to perform or offer commercial services of any kind using PCR, including without limitation reporting the results of purchaser s activities for a fee or other commercial consideration, is hereby granted by implication or estoppel. 6. Uracil-N-glycosylase The use of uracil-n-glycosylase for carryover prevention is licensed by Life Technologies, Inc. under U.S. Patent 5,035,996 and foreign equivalents for research purposes only. No right for use in other applications, including the diagnosis of disease in humans, animals, or plants under any patents owned by Life Technologies, Inc. are covered by the purchase of this product. 7. SYBR Green The SYBR Green dye is sold pursuant to a limited license from Molecular Probes, Inc. under U.S. Patent No. 5,436,134 and 5,658,751 and corresponding foreign patents and patent applications. europe.appliedbiosystems.com Applera Italia Fillale della Applera Europe B.V. Page 25 of 27

26 8. NOTICE TO PURCHASER: Disclaimer of License: MultiScribe TM Reverse Transcriptase These products are optimized for use in the polymerase chain reaction (PCR) and 5 nuclease detection methods covered by patents owned by Roche Molecular Systems, Inc. and F. Hoffmann-La Roche Ltd. No license under these patents to use the PCR Process is conveyed expressly or by implication to the purchaser by the purchase of these products. A license to use the PCR Process for certain research and development activities accompanies the purchase of certain Applied Biosystems reagents when used in conjunction with an authorized thermal cycler, or is available from Applied Biosystems. Further information on purchasing licenses to practice the PCR process may be obtained by contacting the Director of Licensing at Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California or at Roche Molecular Systems, Inc., 1145 Atlantic Avenue, Alameda, California 94501, USA. 9. NOTICE TO PURCHASER: TaqMan Probes TaqMan probes are covered by U.S. Patent 5,723,591 and foreign counterparts and patents pending owned by Applera Corporation, and may be covered by U.S. Patents 5,801,155 and 6,084,102 and foreign counterparts licensed to Applied Biosystems. 10. NOTICE TO PURCHASER: LIMITED LICENSE: rtth DNA Polymerase A license under U.S. Patents 4,683,202, 4,683,195, 4,965,188, 5,407,800, 5,322,770 and 5,310,652 or their foreign counterparts, owned by Roche Molecular Systems Inc. and F. Hoffmann-La Roche Ltd ("Roche"), has an up- front fee component and a running-royalty component. The purchase price of this product includes limited, nontransferable rights under the running-royalty component to use only this amount of the product to practice the Polymerase Chain Reaction ("PCR") and related processes described in said patents solely for the research and development activities of the purchaser when this product is used in conjunction with a thermal cycler whose use is covered by the up-front fee component. Rights to the up- front fee component must be obtained by the end user in order to have a complete license to use this product in the PCR process. These rights under the up-front fee component may be purchased from Applied Biosystems or obtained by purchasing an Authorized Thermal Cycler. No right to perform or offer commercial services of any kind using PCR, including without limitation reporting the results of purchaser s activities for a fee or other commercial ngconsideration, is hereby granted by implication or estoppel. europe.appliedbiosystems.com Applera Italia Fillale della Applera Europe B.V. Page 26 of 27

27 11. NOTICE TO PURCHASER: LIMITED LICENSE: GMO Detection Kits A license under U.S. Patents 4,683,202, 4,683,195, and 4,965,188 or their foreign counterparts, owned by Roche Molecular Systems, Inc. and F. Hoffmann-La Roche Ltd (Roche), has an up-front fee component and a running-royalty component. The purchase price of this product includes limited, non-transferable rights under the running-royalty component to use only this amount of the product to practice the Polymerase Chain Reaction (PCR) and related processes described in said patents solely for genetically modified organism ("GMO") testing and research activities of the purchaser when this product is used in conjunction with a thermal cycler whose use is covered by the up- front fee component. Rights to the up-front fee component must be obtained by the end user in order to have a complete license. These rights under the up-front fee component may be purchased from Applied Biosystems or obtained by purchasing an authorized thermal cycler. No right to perform or offer commercial services of any kind using PCR, including without limitation reporting the results of purchaser s activities for a fee or other commercial consideration, is hereby granted by implication or estoppel. 12. NOTICE TO PURCHASER: LIMITED LICENSE: Pathogen Detection Kits In 11. replace "solely for genetically modified organism ("GMO") testing and research activities" by "solely for the environmental testing, quality control/quality assurance testing, food testing and research activities" 13. NOTICE TO PURCHASER: License Disclaimer: PrepMan Ultra This product conveys no patent rights, expressly or by implication, under any patent or patent application owned by or licensable by Applera Corporation that covers any thermal cycling instrument, apparatus or system, any composition, reagent, or kit, or any process. Specifically, but without limitation, no right, immunity, authorization, or license is granted, expressly or by implication, for the processes of PCR, real-time PCR, reverse-transcription PCR, or the 5 nuclease assay. 14. Micro Fluidic Card Micro Fluidic Card developed in collaboration with 3M Company. 15. Endogenous Control Plates This product is sold under licensing arrangements between Applied Biosystems and Life Technologies, Inc. The purchase of this product includes limited, nontransferable rights under U.S. Patent 5,035,996 and foreign equivalents owned by Life Technologies, Inc. to use only this amount of the product to practice the claims in said patents solely for activities of the purchaser within the field of research. Further information on purchasing licenses under the above patents may be obtained by contacting the Director, Corporate Development at Life Technologies, Inc., 9800 Medical Center Dr., Rockville, MD Further information on purchasing licenses to practice the PCR Process may be obtained by contacting the Director of Licensing at Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California or at Roche Molecular Systems, Inc., 1145 Atlantic Avenue, Alameda, California 94501, USA. europe.appliedbiosystems.com Applera Italia Fillale della Applera Europe B.V. Page 27 of 27