Introducing the MISSION 3 UTR Lenti GoClone. Take the guess work out of your mirna target validation.

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1 Bioidentify. Introducing the MISSION 3 UTR Lenti GoClone. Take the guess work out of your mirna target validation. You can now validate the 3 UTR targets of your favorite mirna with a functional, versatile and quantitative assay. Sigma Life Science and SwitchGear Genomics, have partnered to offer you a collection of lentiviral constructs, each with a unique human 3 UTR cloned downstream of an optimized Renilla luciferase. Identify and validate the 3 UTR targets of your favorite mirna with the powerful new MISSION 3 UTR Lenti GoClone. wherebiobegins.com/lentigoclone biosilencing Sigma, Sigma-Aldrich and MISSION are registered trademarks of Sigma-Aldrich Biotechnology L.P., an affiliate of Sigma-Aldrich Co. GoClone and SwitchGear Genomics are trademarks of SwitchGear Genomics.

2 cell sciences cytokine center Browse our web site of recombinant proteins, including cytokines, growth factors, chemokines and neurotrophins. Daily shipping and competitive pricing are offered. Bulk quantities of many proteins available. Cell Sciences also carries corresponding antibodies and ELISA kits. LIST OF PROTEINS 4-1BBL 4-1BB Receptor 6 Ckine ACAD8 ACAT2 gacrp30/adipolean Activin A ACY1 ADAT1 Adiponectin ADRP AITRL Akt1 Alpha-Feto Protein (AFP) Alpha-Galactosidase A Angiopoietin-1 (Ang-1) Angiopoietin-2 (Ang-2) Angiostatin K1-3 Annexin-V apo-saa Apoliprotein A-1 Apoliprotein E2 Apoliprotein E3 Apoliprotein E4 APRIL Artemin ATF2 Aurora A Aurora B BAFF BAFF Receptor BCA-1 / BLC / CXCL13 BCMA BD-1 BD-2 BD-3 BDNF Bivalirudin BMP-2 BMP-4 BMP-7 BMP-13 sbmpr-1a Brain Natriuretic Protein BRAK Breast Tumor Antigen C5a C5L2 Peptide C-10 C-Reactive Protein C-Src Calbindin D-9K Calbindin D-28K Calbindin D-29K Calmodulin Calcitonin Acetate Carbonic Anhydrase III Carcino-embryonic Antigen Cardiotrophin-1 Caspase-3 Caspase-6 CD4 CD14 CD22 CD40 Ligand / TRAP CD95 / sfas Ligand CD105 /Endoglin CHIPS CNTF Collagen CREB CTACK/CCL27 CTGF CTGFL/WISP-2 CTLA-4/Fc CXCL16 Cytokeratin 8 DEP-1 Desmopressin Disulfide Oxidoreductase E-selectin ECGF EGF Elafin/SKALP EMAP-II ENA-78 Endostatin Enteropeptidase Eotaxin Eotaxin-2 Eotaxin-3 (TSC) EPHB2 EPHB4 Eptifbatide Erk-2 Erythropoietin (EPO) Exodus-2 Fas Ligand Fas Receptor FGF-1 (acidic) FGF-2 (basic) FGF-4 FGF-5 FGF-6 FGF-7/ KGF FGF-8 FGF-9 FGF-10 FGF-16 FGF-17 FGF-18 FGF-19 FGF-20 sfgfr-1 (IIIc) / Fc Chimera sfgfr-2 (IIIc) / Fc Chimera sfgfr-3 / Fc Chimera sfgfr-4 / Fc Chimera sflt-1 (native) sflt-1 (D3) sflt-1 (D4) sflt-1 (D5) sflt-1 (D7) Flt3-Ligand sflt-4 sflt-4/ Fc Chimera Follistatin FSH Fractalkine/ CX3C G-CSF α-galactosidase A Galectin-1 Galectin-3 Gastrointestinal CA GCP-2 GDF-3 GDF-9 GDF-11 GDNF GLP-1 Glucagon Goserelin GM-CSF GPBB GROα GROβ GROγ GRO/MGSA Growth Hormone Growth Hormone BP GST-p21/WAF-1 HB-EGF HCC-1 HGF Histdyl-tRNA synthetase Histrelin HRG1-β1 I-309 I-TAC IFN-α IFN-α A IFN-α 2a IFN-α 2b IFN-β IFN-γ IFN-Omega IGF-I IGF-II proigf-ii IGFBP-1 IGFBP-2 IGFBP-3 IGFBP-4 IGFBP-4 IGFBP-5 IGFBP-6 IGFBP-7 IL-1α IL-1β IL-2 IL-3 IL-4 sil-4 Receptor IL-5 IL-6 sil-6 Receptor IL-7 IL-8 (72 a.a.) IL-8 (77 a.a.) IL-9 IL-10 IL-11 IL-12 IL-13 IL-13 analog IL-15 IL-16 (121 a.a.) IL-16 (130 a.a.) IL-17 IL-17B IL-17D IL-17E IL-17F IL-19 IL-20 IL-22 IL-31 Insulin IP-10 JE JNK2a1 JNK2a2 KC / CXCL1 KGF L-asparaginase LAG-1 LALF Peptide LAR-PTP LC-1 LBP LD-78β LDH LEC/NCC-4 Leptin LIGHT LIX LKM LL-37 Lymphotactin slyve-1 M-CSF MCP-1 (MCAF) MCP-2 MCP-3 MCP-4 MCP-5 MDC (67 a.a.) MDC (69 a.a.) MDH MEC Mek-1 MIA Midkine MIG / CXCL9 MIP-1α / CCL3 MIP-1β / CCL4 MIP-3 / CCL23 MIP-3α / CCL20 MIP-3β / CCL19 MIP-4 (PARC) /CCL18 MIP-5 / CCL15 MMP-3 MMP-7 MMP-13 Myostatin Nanog NAP-2 Neurturin NFAT-1 beta-ngf NOGGIN NOV NP-1 NT-1/BCSF-3 NT-3 NT-4 Ocreotide Oncostatin M Osteoprotegerin (OPG) OTOR Oxytocin p38-α Parathyroid Hormone PDGF-AA PDGF-AB PDGF-BB Persephin PF-4 PIGF-1 PIGF-2 PKA α-subunit PKC-α PKC-γ Pleiotrophin PLGF-1 Polymyxin B (PMB) PRAS40 PRL-1 PRL-2 PRL-3 Prokineticin-2 Prolactin Protirelin PTHrP PTP1B PTP-IA2 PTP-MEG2 PTP-PEST srank srankl RANTES RELM-α RELM-β Resistin RPTPβ RPTPγ RPTPμ SCF SCGF-α SCGF-β SDF-1α SDF-1β Secretin SF20 SHP-2 STAT1 c-src TACI TARC TC-PTP TECK TFF2 TGF-α TGF-β1 TGF-β2 TGF-β3 Thymosin α1 stie-1/fc Chimera stie-2/fc Chimera TL-1A TNF-α TNF-β stnfr1 stnfr2 TPO TRAIL/Apo2L strail R-1 (DR4) strail R-2 (DR5) TSH TSLP TWEAK TWEAK Receptor Urokinase VEGF121 VEGF145 VEGF165 VEGF-C VEGF-C I525 EG-VEGF VEGF-E HB-VEGF-E svegfr-1 svegfr-2 svegfr-3 WISP-1 WISP-2 WISP-3 WNT-1 CELL SCIENCES INC 480 NEPONSET STREET, BUILDING 12A, CANTON, MA INFO@CELLSCIENCES.COM TOLL FREE: (888) TEL: (781) FAX: (781) WEB:

3 Submission deadline August1 Your name here. The GE & Science Prize for Young Life Scientists. Because brilliant ideas build better realities. Imagine standing on the podium at the Grand Hotel in Stockholm, making your acceptance speech for the GE & Science Prize for Young Life Scientists. Imagine having your essay read by your peers around the world. Imagine discussing your work in a seminar with other prize winners and Nobel Laureates. Imagine what you could do with the $25,000 prize money. Now stop imagining. If you were awarded your Ph.D. in molecular biology in 2010, then submit your 1000-word essay by August 1, and you can make it a reality. Want to build a better reality? Go to imagination at work * For the purpose of this prize, molecular biology is defined as that part of biology which attempts to interpret biological events in terms of the physicochemical properties of molecules in a cell. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th Edition). GE Healthcare Bio-Sciences AB, Björkgatan 30, Uppsala, Sweden General Electric Company All rights reserved AB

4 Better Antibodies Better Science Now Available 2500 RABBIT MONOCLONALS Over 9000 Antibodies Total THE RABBIT MONOCLONAL ADVANTAGE RABBIT MONOCLONAL MOUSE MONOCLONAL What is the Rabbit Monoclonal Advantage RECOGNITION AFFINITIES APPLICATIONS IHC Excellent response to a wide range of antigens Limited immuno response Picomolar (10-12 kd M) possible Nanomolar (~10-9 kd M) Westerns,ELISA,Flow Cytometry, IP, IHC, ICC - Excellent results in IHC Westerns,ELISA,Flow Cytometry, IP - Not suitable for mouse studies Rabbit Monoclonal Antibodies (RabMAbs ) provide the combined benefits of superior antigen recognition of the rabbit immune system with the specificity and consistency of a monoclonal antibody, bringing you the highest quality antibody possible. Find out Paraffin-embedded human colon tissue stained with Epitomics CDX2 RabMAb (Cat.# ) and Vendor A s CDX2 Mouse Monoclonal under optimal conditions Find out SPECIAL OFFER $99ANTIBODY SPECIAL For updates and special offers Follow us on: US & Canada Outside NA

5 What color will helpyou today? Abcam offers a variety of conjugated secondary antibodies that complement our products in a wide range of applications. Monoclonal and polyclonal antibodies Large selection of host and target species Conjugated to high quality fluorochromes Pre-adsorbed F(ab) and F(ab ) 2 fragments Learn more about DyLight at DyLight is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries Abcam This image is courtesy of an Abreview in which an E14 mouse brain sample was stained with a primary Rabbit anti- Clim1 antibody. The secondary antibody used was ab96921, a Donkey polyclonal secondary antibody to Rabbit IgG - H&L (DyLight 594) pre-adsorbed against 8 species. The blue staining is DAPI.

6 THE ONE YOU VE BEEN WAITING FOR OneTaq DNA Polymerase The ONE polymerase for your endpoint PCR needs Robust yield with minimal optimization Ideal for routine, AT- or GC-rich templates Hot start and master mix versions available Request a sample at kb M %GC AT-rich Standard GC-rich High GC Standard Reaction Buffer GC Reaction Buffer Plus High GC Enhancer Amplification of a selection of sequences with varying AT and GC content from human and C. elegans genomic DNA, using OneTaq DNA Polymerase.

7 2011 Thermo Fisher Scientific Inc. All rights reserved.copyrights in and to the scientists image are owned by a third party and licensed for limited use only to Thermo Fisher Scientific by Getty Images. Limited by an outdated PCR system? You can run 15 minute PCR protocols and use 50% less consumables and energy by getting away from the old standard. Choose Thermo Scientific Piko Thermal Cyclers, Phusion reagents, and ultra thin wall (UTW) plastic consumables for the most rapid and accurate PCR results. These technologically advanced components can be used individually to improve your current workflow, or as a complete solution for advanced PCR. Ultra fast PCR cycling protocols optimized to <15 minutes 96-well PCR runs using 50% less plastics, reagents and energy PCR kits that allow amplification directly from plant, blood, and tissue samples Start your PCR experiment today Everything for PCR Thermo Scientific PCR portfolio has everything you need for successful PCR including industry leading reagents, high-quality instruments and trusted plastic consumables. Moving science forward

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9 A dream told me to do it. Dr. Carl Alving on his inspiration for inventing the vaccine patch. Carl R. Alving, M.D. Chief of the Department of Adjuvant & Antigen Research, Division of Retrovirology at the Walter Reed Army Institute of Research AAAS member MemberCentral is the new website that looks at science through the eyes of AAAS members. It celebrates their achievements like Dr. Alving s vaccine patch and their shared belief in the transformative power of science. Use MemberCentral to connect with other members, learn about work being done in other fields, and get fresh perspectives on issues ranging from speciation to STEM education. Visit MemberCentral today and get to know the AAAS member community in a whole new way. MemberCentral.aaas.org Blogs I Videos I Webinars I Discounts I Downloads Community

10 EppendorfConsumables it syoursample eppendorf and Eppendorf Tubes are registered trademarks of Eppendorf AG. All rights reserved, including graphics and images. Copyright 2011 by Eppendorf AG. Go for Unaffected Assay Results! Choose the right tubes for your experiments Laboratory consumables can affect bioassays! Reaction tubes for storing and processing samples should be selected carefully as they can influence the qualityandreproducibilityofyourentireworkflow. Original Eppendorf Tubes are produced without additives thathavebeenshowntoinfluencebioassayresults,offer proven mechanical stability, superior technical performanceandbatchcertifiedpuritylevels. OriginalEppendorfTubes : Developedwithyourresearchresultsinmind! No slip agents, no plasticizers or biocides used at any time of the production process No leaching of UV-absorbing substances that can affectdnaquantifications Automated manufacturing under clean-room conditions to eliminate human interaction as source for contamination Experience Eppendorf quality consumables and orderyourfreesampleon: Your local distributor Application Support: support@eppendorf.com Eppendorf AG Germany Tel: Eppendorf North America, Inc. USA Tel: Eppendorf Asia Pacific Headquarters Malaysia Tel:

11 AAAS/Science Business Office Feature LIFE SCIENCE TECHNOLOGIES NEW PRODUCTS DUAL-MEMBRANE SYRINGE FILTERS The Phenex-GF/NY dual-membrane syringe filters are designed for viscous, particulate-laden samples. The new filter incorporates a glass layer, through which the sample passes first for elimination of heavy particulates, increasing the effectiveness of the second, nylon fiber membrane. By removing particulates from the sample, the syringe filter prevents buildup and blockage of chromatography columns and frits while minimizing wear and tear on injection valves. Proper sample filtration can ultimately reduce system downtime and component replacement. Ideal for any applications with highly viscous or heavily particulate-laden samples, such as foods, biological fluids, soil, and fermentation broths, Phenex-GF/NY performs at higher capacity than nylon-only filters and requires less hand pressure to filter the sample. Phenex syringe filters are optimized for superior flow rates and high throughput. Target applications include food and beverage, and environmental testing. Phenomenex For info: PROTEASE INHIBITOR COCKTAIL FOCUS ProteaseArrest is a ready-to-use, 100x concentrated broad range protease inhibitor cocktail that is fully compatible with 2D electrophoresis and subsequent mass spectrometry. The protease inhibitor cocktail contains reversible and irreversible inhibitors of serine, cysteine, calpain, and metalloproteases. Due to the optimized concentration of the various inhibitors, FOCUS ProteaseArrest shows excellent inhibition of protease activities and is therefore suitable for the protection of protein samples from animal tissues, plants, yeast, and bacteria. FOCUS ProteaseArrest is compatible with 2D electrophoresis as it uses an alternative to EDTA as an inhibitor of metalloproteases. The absence of EDTA allows for optimal action of nucleases or removing nucleic acids from the samples. For additional convenience, OneQuant ProteaseArrest is available as a single use aliquot suitable for >95 percent protease inhibition in 10 ml solutions. G-Biosciences For info: KINASE ENRICHMENT KITS The Thermo Scientific Pierce Kinase Enrichment Kits use novel ATPand ADP-based probes developed by ActivX Biosciences to covalently modify active sites of ATPases, including kinases, chaperones, and metabolic enzymes. The probes feature a desthiobiotin tag, a biotin analog, attached to the nucleotide. The nucleotide binds to the active site of the kinase, whereas the desthiobiotin tag allows for capture and enrichment of the bound kinase. The desthiobiotin tag binds less tightly to biotin-binding proteins, making the capture bond easily reversible by biotin displacement, low ph, or heat. The isolated proteins then can be further studied by Western blot analysis or mass spectrometry. The system is useful for inhibitor profiling and IC 50 determinations. The Pierce Kinase Enrichment Kit is available with either an ActivX ATP- or ADP-based probe and includes all labeling and enrichment reagents. Both ActivX Probes are also available separately. Thermo Fisher Scientific For info: THERMO-CONDUCTIVE PLATE HOLDER Used as an interface between a microplate and crushed ice, the BioCision CoolSink thermo-conductive plate holder provides fast equilibration of all wells to below 4ºC and excellent well-to-well temperature uniformity. Many experimental and clinical applications require low-temperature control for culture plates. Placing a plate on BioCision s thermo-conductive CoolSink and then putting the assembly on ice brings all wells to or below 4ºC. This setup provides a very simple way for researchers to ensure that all their samples are treated uniformly with no plate edge effect. CoolSink is an ideal tool for increasing the reproducibility of results in a variety of applications, such as cell-based assays, ELISA, FACS staining and analysis, migration or adhesion studies, temperature shift assays, or any work with temperature-sensitive biomarkers. BioCision For info: PHOSPHOPEPTIDE ENRICHMENT KIT Recently, titanium dioxide (TiO 2 ) has been demonstrated to enrich phosphopeptides from complex peptide mixtures. This methodology has provided a solution for this challenging analytical task; however, the technology has not been optimized. Protea Biosciences has improved upon its previously existing TiO 2 product with the release of the new TiO 2 SpinTip Sample Prep Kit (SP-154). This kit contains tips and reagents for the enrichment of phosphopeptides from complex mixtures such as protein digest samples. The reformulated reagent solutions are specially formulated to maximize the retention of phosphorylated peptides, while simultaneously minimizing the retention of nonphosphorylated peptides. Protea Biosciences For more info: OXYGEN FLASK COMBUSTION UNIT The Oxygen Flask Combustion Unit (OFCU-1) provides a highly safe tool for preparing samples for elemental analysis of halogens, sulfur, phosphorus, and a number of metals using the Schöniger flask method. The Schöniger flask method is a well-proven technique for combusting samples prior to elemental analysis by ion chromatography or titration. The durably constructed OFCU-1 copes easily with a wide range of sample types and is very simple to set up, use, and maintain. Built with operator safety in mind, the OFCU-1 remotely ignites samples inside a safety interlocked chamber using focused infrared heat from two tungsten-halogen lamps. Integral pressure vents ensure safe release of excess reaction pressure. A thick black acrylic observation window is provided on the OFCU-1 to allow users to visually check ignition has taken place. Exeter Analytical For info: Electronically submit your new product description or product literature information! Go to for more information. Newly offered instrumentation, apparatus, and laboratory materials of interest to researchers in all disciplines in academic, industrial, and governmental organizations are featured in this space. Emphasis is given to purpose, chief characteristics, and availability of products and materials. Endorsement by Science or AAAS of any products or materials mentioned is not implied. Additional information may be obtained from the manufacturer or supplier. SCIENCE VOL MAY

12 The Proteomics of Posttranslational Modi cations Unraveling Cellular Signaling Through Mass Spectrometry Webinar June 8, noon ET, 9 am PT, 5 pm UK, 6pm CEST Posttranslational modi cationsincluding phosphorylation, acetylation, and ubiquitinationallow for the ne-tuning of signaling pathways and networks within cells. Mass spectrometry-based proteomics techniques are powerful tools for the investigation of these networks. Dynamic protein phosphorylation is a key cellular regulatory mechanism, and the characterization of global phosphorylation pro les using large-scale proteomic approaches, including antibody-based phosphopeptide enrichment, has proven informative. Acetylation has also been studied using these approachesas histone deacetylase inhibitors gain clinical importance, the need to better characterize their mechanism of action has accelerated lysine acetylation proteomics research. Further, ubiquitination is gaining growing attention as an important mechanism for both signal transduction and proteolytic degradation. Most recently, new ubiquitin af nity reagents, such as the ubiquitin remnant antibody, have become available, allowing for the identi cation of hundreds of ubiquitinylated lysine residues in human cells. Participating Experts Matthias Mann, Ph.D. Max Planck Institute of Biochemistry Martinsried, Germany Samie Jaffrey, M.D., Ph.D. Weill Cornell Medical College New York, NY Akhilesh Pandey, M.D., Ph.D. Johns Hopkins Medical Institutes Baltimore, MD During this webinar, viewers will: Obtain an overview of posttranslational mechanisms and their role in cellular signaling Learn about technologies for studying and characterizing these modi cations REGISTER NOW! Sign Up At: See how the latest technologies are being applied by experts in the eld to cellular signaling research Have the opportunity to put their question to the expert panel live and in real time. Brought to you by the AAAS/Science Business Of ce Webinar sponsored by

13 ELECTROPHORESIS AND BLOTTING // TRANS-BLOT TURBO Efficient protein transfers in 3 minutes. That s transferapidly. The Trans-Blot Turbo transfersystem thenextinnovation inproteintransfersforwesternblotting.getmoreblotsdone faster without compromising on performance and transfer efficiency. Accelerate your western blotting with: Turbo Transfers total protein transfer in as little as 3 min using the convenient and ready-to-use Trans-Blot Turbo Transfer Packs SuperiorTransferEfficiency up to 6x the transfer efficiency compared to the iblot dry blotting system; efficienttransferofhigh-andlow-mwproteins High Throughput transfers up to 4 mini/2 midi gels simultaneously System Flexibility can run traditional semi-dry protocols using standard membranes, paper, and buffers MakethechangetoourTrans-BlotTurbosystem. CallyourBio-Radsalesrepresentativeorvisit Research. Together. A. Trans-Blot Turbo system C. Semi-dry blot B. Tank blot 10 ng 5 ng 2.5 ng 1.25 ng 10 ng 5 ng 2.5 ng 1.25 ng D. iblot system Superior transfer efficiency. Serial dilutions of transferrin were separated on a Criterion TGX 4 20% gel and transferred using four different techniques. A, Trans-BlotTurbosystem(25Vfor7min);B, tank blotting (100 V for 30 min); C, semi-dry (25 V for 30 min); D, iblot(p3for7min). iblot is a registered trademark of Invitrogen Corporation. To find your local sales office, visit In the U.S., call toll free at BIORAD ( ) Visit us at