NIKLAS RYE JØRGENSEN, JULY 2014

Transcription

1 CV NIKLAS RYE JØRGENSEN, JULY 2014

2 Niklas Rye Jørgensen Page 2 INDEX 1. PERSONAL INFORMATION 3 2. PROFESSIONAL QUALIFICATIONS Educational record Special courses Appointments (full time) Appointments (part time, consultancy etc.) 5 3. SCIENTIFIC QUALIFICATIONS Academic degrees Publications Membership of scientific societies 7 4. MANAGEMENT AND ADMINISTRATIVE FUNCTION Education and courses Managerial and administrative experience Chairmanship Membership of professional committees and councils Other honorary offices 9 5. EDUCATION OF POSTGRADUATE STUDENTS AND THE LIKE Supervision of postgraduate students and PhD students Supervision of pregraduate students Contribution to postgraduate courses Pregraduate teaching EVALUATION Membership of scientific editorial board Reviewing of articles Membership of evaluation committees for academic personnel Membership of evaluation committees for academic dissertations Membership of research councils RESEARCH COMMUNICATION Invited lectures at conferences and meetings Chairman at international scientific conferences Other scientific papers and posters Publications and presentations in popular scientific connection RESEARCH FUNDING Funding awards held during the last five years Participation in international research programmes Existing commercial collaborations RESEARCH COLLABORATIONS 25

3 Niklas Rye Jørgensen Page 3 1. PERSONAL INFORMATION Name: Niklas Rye Jørgensen Age: Born 1967 Address: Axel Heides Gade 2, 6. lejl København S Phone: (home) (mobile) [email protected] 2. PROFESSIONAL QUALIFICATIONS 2.1 EDUCATIONAL RECORD Student (Math-Biology) Niels Steensen s Highschool June 1985 Medical Doctor University of Copenhagen June 1992 PhD (Medicine) University of Copenhagen March 2000 Doctor of Medical Science University of Copenhagen October 2005 Specialist Clinical Biochemistry Danish Health Agency March SPECIAL COURSES MANAGEMENT 1. Ledelse, adm. og samarbejde (LAS II) Comwell, Roskilde Oct., 3. Nov Ledelse, adm. og samarbejde (LAS III) Glostrup Park Hotel January Ledertalentudviklingsforløb Region Hovedstaden June OLAU Grundmodul Hindsgavl Castle September Læge og Leder Ledelse af Fagprofessionelle Hindsgavl Castle 26. March -13. June Ledelse af Forskning (Research Management) Region Hovedstaden Fall/winter 2014/2015 CLINICAL BIOCHEMISTRY 1. Endocrinology Domus Medica October Reproductive system Roskilde Hospital August Cardiovascular diseases Rigshospitalet November 4. Metabolism Odense University Hospital October Thrombosis and Haemostasis Esbjerg Hospital October Haematology Hvidovre Hospital November Pharmacology and toxicology Vejlefjord November 2008 PHD COURSES

4 Niklas Rye Jørgensen Page 4 1. Cellular immunology University of Copenhagen November Optical microscopy (34 lessons) Washington University Spring Light microscopy and imaging University of Copenhagen August Reference Manager for Windows Danish National Library 3. October Basic statistics for medical researchers University of Copenhagen January-April Immunochemical methods University of Copenhagen January In vitro gene expression University of Copenhagen February 1999 EU FUNDING RELATED 1. Vejen til en god ansøgning til EU FP7 Eurocenter 19. februar Budgetter og regnskaber i FP7 Eurocenter 21. April Styr dit FP7 projekt sikkert I havn Eurocenter 4. November Snyd ikke dig selv i et FP-7 projekt Eurocenter 5. November 2009 OTHER 1. Courses in sports medicine Danish Soc. for Sports Med Acupuncture basic course Danish Soc. for Acupuncture January Practical use of acupuncture Sheffield, UK February Acupuncture course V.A. Danish Soc. for Acupuncture 30. April-3. May Polymerase Chain Reaction (PCR) Hvidovre Hospital June Patent Conditions (24 lessons) Danish Research Agency November Genetic therapy University of Copenhagen May Laboratory animal science (30 lessons) University of Copenhagen January Bone histomorphometry (ECTS course) Aarhus University October SPSS Basic Uni-C December SPSS Regression analysis Uni-C February SPSS Regression analysis Uni-C May Bone histomorphometry Vienna, Austria 23. May Media Training Course Dethlefsen Communication 30. April 2013 CONFERENCES Annual participation in scientific conferences within the relevant fields including American Society for Bone and Mineral Research (ASBMR), European Calcified Tissue Society (ECTS), Purines, Clinical Biochemistry, Cell Biology etc. 2.3 APPOINTMENTS (FULL TIME) 1. Medicine (7 months) Resident Dept. of Int. Medicine, Fakse Hospital Surgery (6 months) Resident Dept. of Surgery, Fakse Hospital General medicine (6 months) Resident General Practice, Stokkemarke Medicine/endocrinology (12 months) Fellow Dept. of Endocrinology, Hvidovre Hospital Research (57 months) Research fellow, Osteoporosis Res. Center, Copenhagen Mun. Hosp Research (6 months) Visit. res. fellow Washington University in St. Louis, MO, USA Research (39 months)

5 Niklas Rye Jørgensen Page 5 Post doc/group leader Osteoporosis Research Clinic, Hvidovre Hospital Clinical Biochemistry (54 months) Fellow (spec.training) Dept. of Clinical Biochemistry, Hvidovre Hospital Clinical Biochemistry (18 months) Fellow (spec. training) Dept. of Clinical Biochemistry, Roskilde Hospital Research (3 months) Consultant Res. Center for Ageing and Osteop., Glostrup Hosp Clinical Biochemistry (31 months) Consultant Dept. of Clinical Biochemistry, Glostrup Hospital Clinical Biochemistry (31 months per 31. August 2014) Head of Division Division of Clinical Biochemistry, Dept. of Diagnostics, Glostrup Hospital cont. 2.4 APPOINTMENTS (PART TIME, CONSULTANCY ETC.) TEACHING 1. Teacher Niels Steensen s Highscool /Schools in Ishøj Associate professor Institute of Public Health, University of Copenhagen Lecturer (clin. bioch.) Hvidovre Hospital/University of Copenhagen Associate professor Roskilde University Tutor for MDs Div of Clinical Biochemistry, Glostrup Hospital 2011-cont. 6. Assoc. research prof. University of Copenhagen cont. RESEARCH RELATED 1. Medical consultant Zealand Pharma A/S (development of gap junction modifiers as anti-osteoporotic drugs) 2. Lab director Res. Center for Ageing and Osteop, Glostrup Hosp cont. 3. Clinical Assessor Danish Medicines Agency, Div. of Clin. Trials cont. (Evaluation of clinical trial applications and safety) 3. SCIENTIFIC QUALIFICATIONS 3.1 ACADEMIC DEGREES Medical Doctor University of Copenhagen June 1992 PhD (medicine) University of Copenhagen March 2000 Doctor of Medical Science University of Copenhagen October PUBLICATIONS PEER-REVIEWED PUBLICATIONS Total number of peer-reviewed publications (per August 1 st 2014): 83 Number of these within the past five years ( ): 49 SELECTED PUBLICATIONS The publications enclosed with the application have been selected as they are relevant to the research areas in the professorate. Publication # 1-5 relates to cellular and physiological mechanisms for the effects of mechanical loading and unloading, # 6-10 relates to purinergic signaling in health and disease and finally #11-12 relates to clinical use of biomarkers of bone

6 Niklas Rye Jørgensen Page 6 turnover. As the implementation of bone turnover markers is relatively new in our department, we have only started to publish on this recently. and the number of publications in this field is still limited. Please find justification for including the individual publications in brackets adjacent to the publication. 1. Jørgensen NR, Syberg S, Ellegaard M. P2X receptor roles in bone biology. Current Medicinal Chemistry 2014; in press. (Invited review gives the latest overview of the role of P2X receptors in bone biology, health and disease) 2. Kringelbach TM, Aslan D, Novak I, Schwarz P, Jørgensen NR. UTP-induced ATP-release is a finetuned signalling pathway in osteocytes. Purinergic Signalling 2014; XX:xxx-xxx. (Key publication demonstrating the role of purinergic signaling in osteocyte biology) 3. Jørgensen NR, Henriksen Z, Sørensen OH, Eriksen EF, Civitelli R, Steinberg TH. Intercellular calcium signaling occurs between human osteoblasts and osteoclasts and requires activation of osteoclast P2X7 receptors. J Biol Chem 2002; 277: (Provides evidence that purinergic signaling is responsible for mechanically induced signaling between osteoblasts and osteoclast). 4. Jørgensen NR, Henriksen Z, Brot C, Sørensen OH, Eriksen EF, Civitelli R and Steinberg TH. Intercellular calcium waves in human osteoblasts occur by two different mechanisms. J Bone Min Res 2000;15: (Demonstrated the presence of two mechanisms for mechanically induced signaling among osteoblasts) 5. Jørgensen NR, Geist ST, Civitelli R and Steinberg TH. ATP- and gap junction-dependent intercellular calcium signaling in osteoblastic cells. J Cell Biol 1997; 139: (One of the first publications to demonstrate purinergic signaling among osteoblastic cells) 6. Vangsted A, Klausen T, Gimsing P, Abildgaard N, Andersen NF, Gang AO, Holmström M, Gregersen H, Vogel U, Schwarz P, Jørgensen NR. Genetic variants in the P2RX7 gene are associated with risk of multiple myeloma. Eur J Haematol 2014; 93: (This publication shows that genetic alterations in the P2X7 receptor gene has profound consequences for development of myeloma bone disease) 7. Jørgensen NR, Skarratt KK, Husted LB, Tofteng CL, Stokes L, Jensen JEB, Eiken P, Brixen K, Fuller S, Clifton-Bligh R, Gartland A, Langdahl BL, Schwarz P, Wiley JS. Single nucleotide polymorphisms in the P2X7 receptor gene are associated with post-menopausal bone loss and vertebral fractures. Eur J Human Genetics 2012;20; (Demonstrating the role of P2X7 receptor polymorphisms in bone health and disease in the Danish Osteoporosis Prevention Study) 8. Jørgensen NR, Grove EL, Schwarz P, Vestergaard P. Clopidogrel and risk of osteoporotic factors a nationwide cohort study. J Int Med 2012;272: (Showing the bone related side effects of clopidogrel, which was the first drug targeting purinergic receptors) 9. Vestergaard P, Schwarz P, Steinberg TH, Jørgensen NR. Use of the oral platelet inhibitors dipyridamole and acetylsalicylic acid is associated with increased risk of fracture. Int J Cardiol 2011;160: (Our first publication to address the bpne effects of clopidogrel) 10. Ohlendorff SD, Tofteng CL, Jensen JEB, Petersen S, Civitelli R, Fenger M, Abrahamsen B Hermann P, Eiken P, Jørgensen NR. Single nucleotide polymorphisms in the P2X7 gene are associated to fracture risk and to effect of estrogen treatment. Pharmacogenetics Genomics 2007; 17, (The first publication to ever investigate the association between P2 receptor polymorphisms and bone health and disease) 11. Shanbhogue VS, Hansen S, Jørgensen NR, Brixen K, Gravholt CH. Bone geometry, volumetric density, microarchitecture and estimated bone strength assessed by HR-pQCT in Klinefelter syndrome. J Bone Min Res 2014; in press. (Our first collaborative publication on bone turnover markers, made in collaboration with SDU/OUH) 12. Helge EW, Andersen TR, Schmidt JF, Jørgensen NR, Hornstrup T, Krustrup P, Bangsbo J. Recreational football improves bone mineral density and bone turnover marker profile in elderly men. Scand J Med Sci Sports 2014; Suppl.1: (The first publication from a line of collaborative projects on the effects of physical activity on bone mass as measured by bone turnover markers) MOST IMPORTANT REVIEW ARTICLES

7 Niklas Rye Jørgensen Page 7 1. Jørgensen NR, Syberg S, Ellegaard M. P2X receptor roles in bone biology. Current Medicinal Chemistry 2014; in press. 2. Jørgensen NR, Schwarz P. Effects of anti-osteoporosis medications on fracture healing. Curr Reports Osteoporosis 2011; 9, Jørgensen NR, Schwarz P. Osteoporosis in chronic obstructive pulmonary disease patients. Current Opinion Pulmonary Medicine 2008; 14: Jørgensen NR. Short-range intercellular calcium signalling in bone. APMIS 2005; 113 (suppl.118): Jørgensen NR, Steinberg TH, Civitelli R. Osteoblast-Osteoclast Communication. Current Opinion in Orthopedics 1999; 10: OTHER SCIENTIFIC PUBLICATIONS Original articles: 53 Editorials: 1 Proceedings: 2 Reviews: 22 Book chapters: 3 Patent applications: 2 Other publications: 5 Publications submitted or close to submission: 12 TEXT BOOKS AND CONTRIBUTIONS TO TEXT BOOKS 1. Civitelli R, Stains JP, Shin CS, Jørgensen NR. Intercellular junctions and cell-cell communication in the skeletal system. p In: Principles of Bone Biology, eds: Bilezikian JP, Raisz LG Academic Press, San Diego, CA. 2. Jørgensen NR, Steinberg TH. Purinergic signaling in osteoblasts. p In: Nucleotides and regulation of bone cell function. Eds: Burnstock G, Arnett T CRC Press, Boca Raton, FL. 3. Civitelli R, Lecanda F, Jørgensen NR, Steinberg TH. Intercellular junctions and cell-cell communication in bone. p In: Principles of Bone Biology, eds: Bilezikian JP, Raisz LG, Rodan GA Academic Press, San Diego, CA. 3.3 MEMBERSHIP OF SCIENTIFIC SOCIETIES 1. The New York Academy of Sciences 2. European Calcified Tissue Society 3. American Society for Bone and Mineral Research 4. American Society for Cell Biology 5. The Society for Theoretical and Applied Therapy (Jakobinerklubben) 6. The Danish Endocrine Society 7. The Danish Bone Society 8. The Danish Society for Clinical Biochemistry 4. MANAGEMENT AND ADMINISTRATIVE FUNCTION

8 Niklas Rye Jørgensen Page EDUCATION AND COURSES 1. Ledelse, adm. og samarbejde (LAS II) Comwell, Roskilde Oct. and 3. Nov Ledelse, adm. og samarbejde (LAS III) Glostrup Park Hotel January Ledertalentudviklingsforløb Region Hovedstaden June OLAU Grundmodul DADL (Hindsgavl) September Læge og Leder Ledelse af Fagprof. DADL (Hindsgavl) 26. March -13. June 2012 (included exam, 3 ECTS points) 6. Ledelse af Forskning ) Region Hovedstaden Fall/winter 2014/2015 (accepted for participation) 4.2 MANAGERIAL AND ADMINISTRATIVE EXPERIENCE CONTINUOUS MANAGERIAL AND ADMINISTRATIVE FUNCTIONS 1. Head of Division of Clinical Biochemistry (specialeansvarlig overlæge) cont. Glostrup Hospital 2. Lab Director, Research Center for Ageing and Osteoporosis cont. Glostrup Hospital 3. Group leader for own research group (P2 Bone lab) 2002-cont. Osteoporosis Research Clinic, Hvidovre Hospital and Research Center for Ageing and Osteoporosis EXPERIENCE WITH STAFF AND FINANCIAL ADMINISTRATION 1. As group leader of my own research group over the years I have the responsibility for administration of staff and financial issues 2002-cont. 2. Several grants obtained, in which I had the responsibility for administration of finances and for reporting to the funding agencies 2000-cont. 3. Member of the evaluation committee for employment of the head of Dept. of Clinical Biochemistry, Hvidovre Hospital, Denmark Chairman of the evaluation committee for senior consultant for Division of Clinical Biochemistry, Glostrup Hospital, Denmark Chairman of the evaluation committee for chemist at the Division of Clinical Biochemistry, Glostrup Hospital, Denmark Member of evaluation committees for employing technicians 2012-cont. at the Division of Clinical Biochemistry (several) 7. As coordinator and chairman of the board of the ATPBone consortium funded by the EU s 7 th Framework Programme, I had the administrative and financial responsibility for the grant 4.3 CHAIRMANSHIP Chairman of the Board and Scientific Coordinator for the ATPBone Consortium, which was funded by the EU s 7 th Framework Programme 4.4 MEMBERSHIP OF PROFESSIONAL COMMITTEES AND COUNCILS

9 Niklas Rye Jørgensen Page 9 1. Board member of Gruppen af Unge Forskere i H:S (GUF) Member of Steering Committee for development of a joint intranet in Copenhagen Hospital Corporation (H:S) 3. Member of Scientific Advisory Board, Zealand Pharma A/S Member of the Research Council of The Copenhagen Hospital Corporation (H:S) 5. Board member of the Danish Bone Society Member of the Danish Council for Education in Clinical Biochemistry 2006-cont. (Videreuddannelsesrådet, Region Øst) 7. Member of the Committee for Harmonization in Clinical Biochemistry In Region Hovedstaden (Harmoniseringsgruppen) 8. Treasurer of and Danish representative in the Board of the Nordic Society for Clinical Chemistry 9. Member of the Council for Clinical Biochemistry (Sundhedsfagligt Råd) 2012-cont. in Region Hovedstaden 10. Member of the Committee for establishment of the Danish Rheuma 2013-cont. Biobank 11. Co-founder of and member of steering committee for establishment 2013-cont. of a consortium for an inter-european project on diabetic complications and purinergic receptor signaling as drug targets 12. Board member of the Danish Bone Society 2013-cont. 13. Co-opted member of the Committee of Scientific Advisors for the 2013-cont. International Osteoporosis Foundation (IOF) 14. Member of IOF Working Group on Bone Turnover Markers and 2014-cont. Diabetes and Osteoporosis 15. Member of Project Management Team, CoNeXT project, funded by 2013-cont. University of Copenhagen (Puljen for interdiscipl. forskning). 4.5 OTHER HONORARY OFFICES Danish contact for Washington University International Alumni and Development Programs 5. EDUCATION OF POSTGRADUATE STUDENTS AND THE LIKE 5.1 SUPERVISION OF POSTGRADUATE STUDENTS AND PHD STUDENTS SUPERVISION OF PHD STUDENTS 1. Stine Ohlendorff (MSc) on her project: Single nucleotide polymorphisms in the P2X7 receptor: association with bone mass and fracture risk. Roskilde University PhD awarded March Was the main supervisor, but not the university supervisor. 2. Susanne Syberg Hansen (MSc) on her project: Characterization of a novel P2X7 knockout model, implicates a role for P2X7 in bone metabolism. Roskilde University (she had maternity leave x 2). PhD awarded February Served as main supervisor, but not the university supervisor. 3. Tina Marie Kringelbach (MSc) on her project: P2 purinergic receptors in osteocyte signalling. University of Copenhagen (she had maternity leave x 2). PhD awarded June Served as main supervisor, but not the university supervisor.

10 Niklas Rye Jørgensen Page Katarzyna Gurzawska (cand. odont.) on her project: Nanocoating of implant surfaces with pectin rhamnogalacturonan-is. University of Copenhagen PhD awarded March Co-supervisor. 5. Maria Ellegaard Larsen (MSc) on her project: Parathyroid hormone and bone healing. University of Copenhagen PhD awarded May Co-supervisor. 6. Torben Madsen Kvist (MD) on his project: The role of P2X7 receptors in immune-mediated bone loss. University of Copenhagen Thesis awaiting submission. Co-supervisor. 7. Camilla Albeck Neldam (cand. odont.) on her project: 3D microarchitecture rods, plates and µ-gap of peri-implant hard tissues evaluated by synchrotron µct radiation and histomorphometry. University of Copenhagen 2014-cont. Principal supervisor. 8. Morten Dahl (cand. odont.) on his project: Mesenchymal stem cell osteoblast mineralization. University of Copenhagen 2013-cont. Principal supervisor. SUPERVISION OF POST DOCS 1. Susanne Syberg Nielsen on several projects: a. P2X7 antagonists in the treatment of osteoporosis b. The negative effects on bone of platelet inhibitors targeting the P2Y12 receptor c. Bone turnover marker measurements stability, validity and reference ranges d. P2X7 receptor polymorphisms and their effects on bone cell function 2. Maria Ellegaard Larsen on several projects a. Mechanisms for loading and unloading effects on bone with special focus on purinergic signalling b. Role of P2 receptor polymorphisms in osteoporosis in men c. 3D methods to enhance osteocyte culturing and phenotype in vitro SUPERVISION OF MEDICAL DOCTORS 1. Anne Louise Knudsen, MD during her specialist training at Division of Clinical Biochemistry, Glostrup Hospital Young Bae Villy Lee Hansen, MD during his specialist training at Division of Clinical Biochemistry, Glostrup Hospital SUPERVISION OF PREGRADUATE STUDENTS SUPERVISION OF PREGRADUATE MASTER PROJECTS 1. Stefan Cuoni Teilmann BSc master project: Gap junction openers: Effects on intercellular communication in bone cells. Roskilde University Main supervisor. 2. Susanne Syberg Hansen BSc master project: The synthetic peptide ZP123 increases gap junctional intercellular communication in human osteoblasts. Roskilde University Main supervisor. 3. Rikke Rye Aarup (BSc) master project: Single nucleotide polymorphisms in the purinergic P2Y2 receptor and association to bone status. Roskilde University Main supervisor. 4. Maria Ellegaard Larsen (BSc) master project: The effect of amylin(1-8) on bone in ovariectomized rats. University of Copenhagen Co-supervisor. 5. Tina Marie Kringelbach (BSc) master project: The expression, functions and regulation of P2 purinergic receptors in osteocytes. Roskilde University Main supervisor. SUPERVISION OF OTHER PREGRADUATE PROJECTS 1. Stefan Cuoni Teilmann (BSc) project: Intercellular calcium signaling in osteoblast-like cells: An investigation of gap junction mediated calcium wave propagation. Roskilde University Main supervisor.

11 Niklas Rye Jørgensen Page Barbara Rubek Nielsen (stud.med.) OSVAL II project: Management of risk of glucocorticoid induced osteoporosis due to systemic administration in general practice in Denmark. University of Copenhagen Co-supervisor. 3. Marie Solgaard (stud.med.) OSVAL II project: P2 purinergic receptors in human osteoblasts. University of Copenhagen Main supervisor. 4. Maria Ellegaard Larsen (BSc) project: The OPG/RANKL system and the role in regulation of bone remodeling. University of Copenhagen Co-supervisor. 5. Zanne Henriksen (BSc) project: Calcium signaling during osteoblasts differentiation. Roskilde University Main supervisor. 6. Anne Qvist Rasmussen (BSc) project: Mutations in the calcium sensing receptor. Roskilde University Main supervisor. 7. Nina Geiker and Rikke Larsen (BSc) project: Vitamin D status in elite athletes. University of Copenhagen Co-supervisor. 8. Lasse Maretty Sørensen (stud.med.) OSVAL I project: Adipocyte-osteoblast transdifferentiating potential. University of Copenhagen Main supervisor. 9. Tore Lefolii (stud.odont.) project: Tooth implants in osteoporotic rabbits. University of Copenhagen Co-supervisor. 10. Derya Aslan, Mille Dahl Andersen, Lene Bjerring Gede, Sara Rubek Jørgensen and Tine Kellemann Hansen (BSc) project: Osteoporosis and parathyroid hormone. Roskilde University Main supervisor. 11. Morten Dahl (stud. odont.) scholarship project: Titanium granules and the effect on differentiation of adipocyte progenitors into osteoblasts. University of Copenhagen Co-supervisor. 12. Jan Larsen, Barakat Ali and Thomas Jensen (Bsc) project: Mechanotransduction in osteocytes. Roskilde University Main supervisor. 5.3 CONTRIBUTION TO POSTGRADUATE COURSES ORGANIZER OF POSTGRADUATE COURSES 1. Member of the board/steering group for the PhD school Skeletal Sciences, University of Copenhagen, Member of the board for the PhD school Basic and Clinical Research in Musculoskeletal and Oral Sciences. From 2014-cont. 3. Co-organizer of A-course in clinical biochemistry: Haematology, Hvidovre Hospital November Co-organizer of and course facilitator of compulsory course in research methods (Statistics) for physicians in specialist training (Obligatorisk Forskningstræningskursus for læger I hoveduddannelsesforløb). Three-day course. Has done this app. 25 times since Co-organizer of PhD course: Basic Bone Biology. PhD school Skeletal sciences, University of Copenhagen October Co-organizer of PhD course: Physical activity: Bone and muscle in the old and oldest old. PhD school Skeletal sciences, University of Copenhagen April Co-organizer of A-course in clinical biochemistry: Endocrinology. Responsible for planning the session on bone metabolism and vitamin D. Vejle October Member of working group planning and evaluating the compulsory research training courses (obligatorisk forskningstræningskurser) Modul II. From 2009-cont. ORGANIZER OF SCIENTIFIC MEETINGS AND CONFERENCES 1. Organizer of symposium on bone in tribute to Dr. Ole Helmer Sørensen. Hvidovre Hospital, August Organizer of meeting on Intercellular communication in bone in the Danish Bone Society. Hvidovre Hospital, November 2003.

12 Niklas Rye Jørgensen Page Organizer of meeting on In vitro and in vivo models in osteoporosis and bone research in the Danish Bone Society. Hvidovre Hospital, April Organizer of ATPBone minisymposium at Purines Copenhagen, June Organizer of meeting on Physical activity and osteoporosis in the Danish Bone Society. Glostrup Hospital, November Co-organizer of meeting on The calcium-sensing receptor in the Danish Bone Society. Glostrup Hospital, May TEACHING IN POSTGRADUATE COURSES (PHD) 1. Calcium in the cross-talk between bone cells. PhD course at DIBIT Research Institute, San Raffaele Hospital, Milan, Italy. April Intercellular calcium signaling in bone. PhD course on Bone biology at Aarhus University, Denmark. October Intercellular calcium signaling in bone or how exercise builds strong bones. PhD course in Bone biology at Aarhus University, Denmark. October Intercellular signaling in bone. PhD course on Bone Biology, PhD school of Skeletal Science at University of Copenhagen, Denmark. October Regulation of synthesis and degradation of bone matrix. PhD course on Matrix biology at Faculty of Health Sciences, University of Copenhagen, Denmark. October Regulation of synthesis and degradation of bone matrix. PhD course on Matrix biology at Faculty of Health Sciences, University of Copenhagen, Denmark. November TEACHING IN POSTGRADUATE COURSES (COURSES FOR PHYSICIANS IN SPECIALTY TRAINING) 1. The calcium homeostasis. A-course on Endocrinology for physicians in training in Clinical Biochemistry, Vejle, Denmark. November Calcium signaling in bone cells-presentation of a research project. A-course on Endocrinology for physicians in training in Clinical Biochemistry, Vejle, Denmark. November Basic principles of laboratory research. Compulsory research course for physicians in specialty training (Obligatorisk Forskningstræningskursus Modul 1), University of Copenhagen, Denmark. Spring Statistics in medical research. Compulsory research course for physicians in specialty training (Obligatorisk Forskningstræningskursus Modul 2), Roskilde, Denmark. App. 3-4 annual courses from 2007 and onwards. 5. The calcium homeostasis. A-course on Endocrinology for physicians in training in Clinical Biochemistry, Vejle, Denmark. November Osteoporosis and calcium metabolic diseases. A-course for endocrinologists in training (Dansul course), Hvidovre Hospital, Denmark The use of biochemical markers of bone turnover. A-course for endocrinologist in training. Køge Hospital, Denmark. October How to write a scientific paper, Regulatory issues, and The Research Protocol. Compulsory research course for physicians in specialty training (Obligatorisk Forskningstræningskursus Modul 1), University of Copenhagen, Denmark. March Calcium homeostasis and Bone turnover markers. A-course in clinical biochemstry: Endocrinology for physicians in training in Clinical Biochemistry, Vejle, Denmark. October OTHER POSTGRADUATE TEACHING 1. Osteoporosis prevention, diagnosis, and treatment. Teaching of General Practitioners, Maribo, Denmark. October Osteoporosis. Teaching of health personnel, Kokkedal Health Center, Denmark Hormone replacement therapy vs. selective estrogen modulators (SERMs). Course for product specialists, Roche Pharmaceuticals, Hvidovre, Denmark. August 1998.

13 Niklas Rye Jørgensen Page Clinical trials and statistics. Course for allied health personnel (nurses, physical therapists etc.) Clinical decisions and research methodologies. Danish Institute for Health and Nursing Research, Copenhagen and Sønderborg, Denmark. 2000, 2001, Gap junctions and ATP receptors roles in the regulation of bone turnover. Teaching of academics involved in research at the Dept. of Clinical Biochemistry, Hvidovre Hospital, Denmark. March Future strategies in the bone cell lab. Teaching of academics involved in research at the Dept. of Clinical Biochemistry, Hvidovre Hospital, Denmark. April Biological fluids. Course for technicians, Dept. of Clinical Biochemistry, Hvidovre Hospital, Denmark. Spring Osteoporosis. Course for technicians, Dept. of Clinical Biochemistry, Hvidovre Hospital, Denmark. Spring Zink. Course for technicians, Dept. of Clinical Biochemistry, Hvidovre Hospital, Denmark. Spring Training of patients with osteoporosis. Course for physical therapists on Training of Elderly, Bispebjerg Hospital, Denmark. February Osteoporosis from basic science to clinical applications. Teaching of physicians at Hvidovre Hospital, Denmark. Spring Calcium homeostasis. Staff meeting at Dept. of Clinical Biochemistry, Hvidovre Hospital, Denmark. Fall Osteoporosis. Staff meeting, Dept. of Clinical Biochemistry, Roskilde Hospital, Denmark. January Calcium signaling among bone cells. Staff meeting, Dept. of Clinical Biochemistry, Roskilde Hospital, Denmark. February Analysis of monoclonal components in multiple myeloma. Staff meeting, Dept. of Clinical Biochemistry, Roskilde Hospital, Denmark. June How to write a successful application and how to get the best possible start of the project. Meeting on the Health Programme under the EU Framework Programme 7 arranged by the Danish Agency for Science, Technology and Innovation, Copenhagen, Denmark. August How to steer a Framework Programme 7 project experiences from a coordinator. Course on European FP7-projects arranged by Eurocenter, Copenhagen, Denmark. November How to steer a Framework Programme 7 project experiences from a coordinator. Course on European FP7-projects arranged by Eurocenter, Copenhagen, Denmark. May How to write a successful application and how to get the best possible start of the project. Meeting on the Health Programme under the EU Framework Programme 7 arranged by the Danish Agency for Science, Technology and Innovation, Copenhagen, Denmark. August Bone biology. Staff meeting at Division of Clinical Physiology and Nuclear Medicine, Glostrup Hospital, Denmark. May How to steer a Framework Programme 7 project experiences from a coordinator. Course on European FP7-projects arranged by Eurocenter, Copenhagen, Denmark. June How to steer a Framework Programme 7 project experiences from a coordinator. Course on European FP7-projects arranged by Eurocenter, Copenhagen, Denmark. October The use of biochemical markers of bone turnover. Teaching of physicians at Dept. of Endocrinology, Gentofte Hospital, Denmark. May Bone and bone turnover markers. Teaching of technicians in Biolab, Division of Clinical Physiology and Nuclear Medicine, Glostrup Hospital, Denmark. May 2014.

14 Niklas Rye Jørgensen Page PRE-GRADUATE TEACHING 1. Teaching of medical students, University of Copenhagen/Hvidovre Hospital in Clinical Biochemistry. Topics to include: Disorders of calcium homeostasis, Bone diseases, Hepatic and Biliary diseases, Renal diseases. 6. EVALUATION 6.1 MEMBERSHIP OF SCIENTIFIC EDITORIAL BOARD 1. Member of Editorial Board for Frontiers in Bone Endocrinology 2011-cont. 2. Lead Guest Editor for Journal of Osteoporosis REVIEWING OF ARTICLES 1. American Journal of Physiology Cell Physiology 2. Journal of Bone and Mineral Research 3. Bone 4. Calcified Tissue International 5. Purincergic Signalling 6. Frontiers in Bioscience 7. Frontiers in Endocrinology 8. Thorax 9. Hormone and Metabolic Research 10. Cell Biology International 11. Trends in Pharmaceutical Sciences 12. Cell and Tissue Research 13. Pharmacological Research 14. Sports Medicine 15. Acta Physiologica 16. Scandinavian Journal of Clinical and Laboratory Investigation (SJCLI) 17. WIRE s Membrane Transport and Signaling 18. Yonsei Medicinal Journal 19. Journal of Innate Immunity 6.3 MEMBERSHIP OF EVALUATION COMMITTEES FOR ACADEMIC PERSONNEL 1. Member of the evaluation committee for the head of Dept. of Clinical Biochemistry, Hvidovre Hospital, Denmark Chairman of the evaluation committee for senior consultant for Division of Clinical Biochemistry, Glostrup Hospital, Denmark Chairman of the evaluation committee for chemist at the Division of Clinical Biochemistry, Glostrup Hospital, Denmark Member of evaluation committee for employment of two PhD s and one post doc at Molecular Sleep Lab, Glostrup Hospital, Denmark MEMBERSHIP OF EVALUATION COMMITTEES FOR ACADEMIC DISSERTATIONS 1. Morten Karsdal (MSc) PhD thesis at SDU October Bodil-Cecilie Søndergaard (MD) PhD thesis at SDU September Sune Land Bloch (MD) PhD thesis at University of Copenhagen December 2010

15 Niklas Rye Jørgensen Page Pia Rosgaard Jensen (MSc) PhD thesis at SDU (revised) 5. Stinus Hansen (MD) PhD thesis at SDU January Olena Kucheryavenko (MSc) PhD thesis at SDU November 2013 Since 2007 (March) external examiner at Aarhus University in the Biological Sciences. Also served as external examiner at University of Copenhagen and at Roskilde University at several graduate exams. 6.5 MEMBERSHIP OF RESEARCH COUNCILS, SCIENTIFIC FUND COMMITTEES ETC. MEMBERSHIP OF RESEARCH COUNCILS 1. Member of the Research Council of The Copenhagen Hospital Corporation (H:S Sundhedsvidenskabelige Forskningsråd) 2. Member of the Research Council at Glostrup Hospital 2014-cont. AD HOC EVALUATION OF GRANT APPLICATIONS 1. Research Grants Council Hong Kong North West Cancer Research Fund Scientific Committee Arthritis Research Campaign UK British Lung Foundation Arthritis Research UK Foundation RESEARCH COMMUNICATION 7.1 INVITED LECTURES AT CONFERENCES AND MEETINGS INTERNATIONAL CONFERENCES AND MEETINGS 1. ATP as extracellular messenger in intercellular calcium waves in osteoblastic cells. Invited lecture at mini-symposium on cell signaling, Washington University in St. Louis, MO; USA. April Osteoblast communication-a role for calcium waves. Invited lecture at mini-symposium on cell signaling. Washington University, St. Louis, USA. July Osteoclast communication. Invited lecture at Washington University, St. Louis, USA. August Status on calcium waves in human bone cells. Invited lecture at Washington University, St. Louis, USA. September The role of gap junctional communication in bone diseases. Invited lecture at the First international meeting in consortium on the establishment of a Network of Excellence for the EU s 6 th FP. Paris September The role of purinergic receptors in bone biology. Invited lecture at seminar series on Progress in Cell Biology and Cancer. University of Navarra, Pamplona, Spain. April Calcium in the cross-talk between bone cells. Invited lecture at the Congress on Calcium in Health and Disease. Rovaniemi, Finland. July The role of nucleotides and P2 receptors in intercellular calcium signaling in bone. Invited lecture at the 8 th International Symposium on Adenine and Adenosine Nucleotides, Ferrara, Italy. May Cellular signaling in bone changes during aging and bone diseases. Invited lecture at the IARU Workshop on Ageing, longevity, and health. Copenhagen, Denmark. October Purinergic signaling in the regulation of bone homeostasis. Invited lecture at the 39 th Sandbjerg Meeting on Membrane Transport, Sandbjerg, Denmark. May 2007.

16 Niklas Rye Jørgensen Page P2 receptor single nucleotide polymorphisms and bone metabolism. Invited lecture at the Purines 2008 conference. Copenhagen, Denmark. July P2 receptor SNPs are associated with bone mass and bone strength after menopause. Invited lecture at the ATPBone symposium, University of Liverpool, UK. November Intercellular calcium signaling and mechanotransduction in bone. Invited lecture at seminar at University of Sheffield, UK. November P2X7 receptors and bone Invited lecture at the European Calcified Tissue Society conference, Stockholm, Sweden. May P2X7 antagonists a novel anabolic treatment for osteoporosis?. Invited lecture at the Joint meeting of the German-Italian Purine Club, Rimini, Italy. September The platelet-inhibitor clopidogrel increases risk of bone fractures in stroke patients. Selected for oral presentation at the annual meeting in The American Society for Bone and Mineral Research, Baltimore, USA. October P2X and P2Y receptor polymorphisms and bone health and disease. Invited lecture at the Purines 2014 conference, Bonn, Germany. July NATIONAL CONFERENCES AND MEETINGS 1. The use of video imaging for examination of bone cells. TNF-alfa as modulator of bone cell activity. Invited lecture at scientific meeting at the Osteoporosis Research Center, Copenhagen Municipal Hospital, Copenhagen, Denmark. December Intercellular calcium waves. Invited lecture at staff-meeting, RHIMA Center, Rigshospitalet, Copenhagen, Denmark. February Intercellular calcium waves in osteoblastic cells. Invited lecture in meeting of The Danish Society for Molecular Gerontology, Aarhus, Denmark. March Calcium waves among bone cells. Invited lecture in meeting of the Danish Bone and Tooth Society, Copenhagen, Denmark. March Intercellular calcium signals as communication among human bone cells. Invited lecture in mini-symposium on calcium. Dept. of Nephrology, Rigshospitalet, Copenhagen, Denmark. September Bone cell biology changes in osteoporosis. Invited lecture at mini-symposium on osteoporosis, Copenhagen, Denmark. May The effect of gap junction openers on bone cells in vitro. Invited lecture in meeting on gap junction openers arranged by Statens Serum Institute and Zealand Pharma A/S, Holte, Denmark. November Bone cells past, now or never. Invited lecture at symposium, Hvidovre Hospital, Hvidovre, Denmark. August The P2X7 ko mouse and bone. Invited presentation at meeting with GlaxoSmithKline, Harlow, UK. August The P2X7 ko mouse and bone. Invited presentation at meeting with Rheoscience, Glostrup, Denmark. September Gap junctions and bone status on research. Invited presentation at Zealand Pharma A/S, Glostrup, Denmark. February Short-range intercellular calcium signaling in bone. Invited lecture at meeting in Dept. of Endocrinology, Odense University Hospital, Odense, Denmark. June Osteoporosis presentation of a specialty. Invited presentation at meeting in the Laboratory Center, Hvidovre Hospital, Hvidovre, Denmark. August The effect of gap junction modifiers on bone in vitro and in vivo. Invited presentation at meeting in Zealand Pharma A/S, Glostrup, Denmark. March Short-range intercellular calcium signaling in bone. Presentation in relation to the defense of my doctoral thesis. University of Copenhagen, Denmark. October The P2X7 receptor and hip fractures. Invited lecture in relation to project meeting at Kolding Hospital, Denmark. January 2006

17 Niklas Rye Jørgensen Page The P2X7 receptor and osteoporosis. Invited lecture at meeting at Glostrup Hospital, Denmark. March The role of nucleotides and P2 receptors in intercellular calcium signaling in bone. Invited lecture at seminar in Copenhagen Pharmaceutical University, Denmark. June PTH(1-34) but not alendronate increases bone quality in trabecular bone in a rat model of osteoporosis. Glostrup Hospital, Denmark. September Calcium signaling in bone cells. Invited lecture at meeting in Hvidovre Hospital, Denmark. November Short-range intercellular calcium signaling in bone. Invited lecture at meeting in The Danish Bone Society, Aarhus University, Denmark. November Short-range intercellular calcium signaling in bone. Invited lecture at seminar at Aarhus University, Denmark. May Immobilization and osteoporosis from laboratory to the clinic. Invited presentation at staff-meeting, Roskilde Hospital, Denmark. Spring P2 receptors and bone. Invited lecture at scientific meeting in Dept. of Clinical Biochemistry, Glostrup Hospital, Denmark. July How to write a good protocol for clinical trials. Invited lecture at meeting on clinical trials in the Danish Pharmaceutical Society, University of Copenhagen, Denmark. Fall Training of osteoporosis patients. Invited lecture at the annual Danish Congress of Sports Medicine, Kolding, Denmark. February The use of biochemical bone markers. Invited lecture at Eli Lilly Annual PTH Symposium, Copenhagen, Denmark. March The use of bone turnover markers in Denmark: A biochemical perspective. Invited lecture at meeting in The Danish Bone Society, Odense, Denmark. May Adynamic bone disease the view from the clinical biochemistry lab. Invited lecture in Multidisciplinary Gentofte Symposium on adynamic bone disease, Gentofte Hospital, Denmark. November The platelet inhibitor clopidogrel increases risk of bone fractures. Invited lecture at scientific meeting at Dept. of Clinical Biochemistry, Rigshospitalet, Copenhagen, Denmark. March CHAIRMAN AT INTERNATIONAL SCIENTIFIC CONFERENCES 1. Chairman at the European Calcified Tissue Society conference in the sessions Cell-cell communication and Osteoclast biology. Copenhagen, May Chairman at the ATPBone minisymposium in relation to Purines Copenhagen June Chairman at the Purines 2008 conference in the session Purinergic signaling in bone and skeletal muscle. Copenhagen. June Chairman at the ATPBone symposium in Liverpool, UK. November Chairman at the ATPBone symposium in Maastricht, Netherlands, May Chairman at the ATPBone symposium in Brussels, Belgium, November Chairman at the ATPBone symposium in Cardiff, UK. November Chairman at the Purines 2014 conference in the session Purinergic receptor polymorphisms and their pathological implications. Bonn, Germany. July OTHER SCIENTIFIC PAPERS AND POSTERS 1. Sørensen OH, Kollerup G, Storm T, Thamsborg G, Jørgensen NR, Genant HK. Effect of longterm etidronate treatment on bone mass and spinal fracture rate. Osteoporosis International, Amsterdam, The Netherlands, May 18-23, 1996.

18 Niklas Rye Jørgensen Page Short-term in vivo reliability of bone mass and soft tissue variables assessed by the Norland XR-26 scanner. Jørgensen NR, Jensen JEB, Madsen OR, Sørensen OH, Osteoporosis International, Amsterdam, The Netherlands, May 18-23, Jørgensen NR, Geist S, Civitelli R, Steinberg TH. Intercellular calcium waves in osteoblastic cell lines occur by two different mechanisms. American Society for Bone and Mineral Research, Seattle, WA, September Jørgensen NR, Geist S, Civitelli R, Steinberg TH. ATP-dependent and gap junction-dependent intercellular calcium signalins in osteoblastic cells. American Society of Cell Biology, San Francisco, CA, December Brot C, Jørgensen NR, Hansen B, Sørensen OH. Reduced PTH levels among tobacco consuming perimenopausal women. European Calcified Tissue Society meeting, Vienna Jørgensen NR, Henriksen Z, Eriksen EF, Sørensen OH, Civitelli R, Steinberg TH. Intercellular calcium signalling between human osteoblastic and osteoclastic cells. American Society for Bone and Mineral Research, San Francisco, CA, December Teilmann SC, Jørgensen NR, Sørensen OH. Mechanisms of calcium signalling in osteoblastlike cells. IIIrd Baltic Bone Conference, Ronneby, Sweden, August Rasmussen AM, Jørgensen NR, Hansen B, Sørensen HA, Sørensen OH. IIIrd Baltic Bone Conference, Ronneby, Sweden, August Teilmann SC, Henriksen Z, Sørensen OH, Civitelli R, Steinberg TH, Jørgensen NR. Intercellular calcium signaling in the osteoblast-like cell line ROS 17/2.8 requires activation of voltagegated calcium channels. American Society for Bone and Mineral Research, St. Louis, MO, September Jørgensen NR, Henriksen Z, Sørensen OH, Civitelli R, Steinberg TH. In vitro differentiation of human osteoblast-like cells alters the predominant mechanism of intercellular calcium wave propagation. American Society for Bone and Mineral Research, St. Louis, MO, September Jørgensen NR, Henriksen Z, Eriksen EF, Sørensen OH, Civitelli R, Steinberg TH. Co-cultures of human osteoblasts and osteoclasts can propagate intercellular calcium signals bidirectionally, and purinergic receptors seem to be involved in the signalling. Conference on nucleotides and purinergic receptors, Ferrara, Italy, September Teilmann SC, Henriksen Z, Meier E, Petersen JS, Sørensen OH, Jørgensen NR. The gap junction opener ZS enhances intercellular communication in osteoblastic cells. Gap junction meeting, Honolulu, HI, August Jørgensen NR, Teilmann SC, Henriksen Z, Civitelli R, Sørensen OH. Steinberg TH. Mechanisms of calcium signaling in human bone marrow-derived stromal cells. American Society for Bone and Mineral Research, San Antonio, TX, September Petersen JS, Kjølbye AL, Larsen BD, Harlow KW, Jepsen T, Meier E, Vinge MM, Nielsen MS, Holstein-Rathlou NH, Xing D, Martins JB, Teilmann SC, Sørensen OH, Jørgensen NR. Antiarrhythmic effects of the gap junction opener ZP123. Scandinavian Physiological Society Meeting, Århus, Denmark, Ohlendorff S, Friberg-Hitz M, Tofteng CL, Jensen JEB, Jørgensen NR. A specific mutation in the P2X7 receptor DNA sequence may be responsible for impaired bone quality. European Calcified Tissue Society, Rome, Italy, May Henriksen Z, Sørensen OH, Jørgensen NR. Dexamethasone and BMP-2 induce a more differentiated osteoblast phenotype in human bone marrow-derived stromal cells. European Calcified Tissue Society, Rome, Italy, May Hansen SS, Henriksen Z, Petersen JS, Sørensen OH, Jørgensen NR. The AAP analogue ZP123 increases intercellular coupling in human osteoblasts in vitro. European Calcified Tissue Society, Rome, Italy, May Jørgensen NR, Steinberg TH, Henriksen Z, Ohlendorff SD, Jensen JEB, Chessell IP, Sørensen OH. The purinergic P2X7 receptor is an inhibitor of bone resorption in mice. American Society for Bone and Mineral Research, Minneapolis, MN, September 2003.

19 Niklas Rye Jørgensen Page Jørgensen NR. Calcium in the cross-talk between bone cells. The congress on Calcium in Health and Disease, Rovaniemi, Finland. July Ohlendorff SD, Jørgensen NR. The Glu496Ala polymorphism in the P2X7 receptor and bone loss. Meeting in the Danish Bone Research Society, Århus, Denmark, April Petersen S, Henriksen Z, Ohlendorff SD, Jørgensen NR. Validation of a histomorphometric method to study bone formation and bone resorption in mice. European Calcified Tissue Society, Nice, France, June Furlan F, Jørgensen NR, Rubinacci A, Heagen E, Villa I, Blasi F. Involvement of the urokinase receptor in bone homeostasis. American Society for Bone and Mineral Research, Seattle, WA, September Solgaard M, Henriksen Z, Jørgensen NR. P2 receptors are involved in the regulation of proliferation of human osteoblastic cells. American Society for Bone and Mineral Research, Seattle, WA, September Hansen SS, Ohlendorff SD, Henriksen Z, Teilmann J, Jørgensen NR. Variability in bone status among inbred strains of mice. American Society for Bone and Mineral Research, Seattle, WA, September Henriksen Z, Thorklidsen C, Jensen JEB, Jørgensen NR. PTH increases bone mineral density and resistance to mechanical load in a rat model of osteoporosis. American Society for Bone and Mineral Research, Seattle, WA, September Ohlendorff SD, Petersen S, Tofteng CL, Jørgensen NR. Effect of the P2X7 Glu396Ala polymorphism on osteoclast phenotype and survival. American Society for Bone and Mineral Research, Seattle, WA, September Aaboe M, Bire K, Jørgensen NR, Terheyden H, Acil Y. Cultivation of minipig bone cells for bone tissue engineering: some pitfalls some solutions. Meeting on jaw surgery, Amsterdam, June Nielsen BR, Jørgensen NR, Schwarz P. Primary and secondary prophylaxis to the use of systemic glucocorticoid in primary health care. American Society for Bone and Mineral Research, Nashville, TN, September Petersen S, Hansen SS, Jørgensen NR. Validation of histomorphometric indices in the vertebral spine of C57/BL mice. American Society for Bone and Mineral Research, Nashville, TN, September Furlan F, Jørgensen NR, Jensen JEB, Rubinacci A, Mrak E, Marenzana M, Blasi F. Urokinase receptor is involved in bone remodelling by influencing osteoclast resorption. American Society for Bone and Mineral Research, Nashville, TN, September Furlan F, Jørgensen NR, Jensen JEB, Mrak E, Rubinacci A, Verde P, Blasi F. Urokinase receptor is involved in bone remodelling by influencing osteoclast resorption. European Calcified Tissue Society, Prague, Czech Republic, May Jørgensen NR. The role of P2 receptors and nucleotides in intercellular signalling in bone. 8th International Symposium on Adenine and Adenosine Nucleotides. Ferrara, Italy. May Henriksen Z, Nissen N, Jørgensen NR. Functional P2X7 purinergic receptors are expressed in differentiated human osteoblasts. American Society for Bone and Mineral Research, Philadelphia, PA, September Jørgensen NR, Thorkildsen C, Jensen JE, Petersen JS, Schwarz P. PTH increases bone mineral density and resistance to mechanical load in a rat model of osteoporosis. American Society for Bone and Mineral Research, Philadelphia, PA, September Qvist A, Jørgensen NR, Schwarz P. Sequencing of the promoter region and exons 1a and 1b in the human calcium-sensing receptor gene. European Calcified Tissue Society meeting, Copenhagen, Denmark, May Henriksen Z, Nissen N, Schwarz P, Larsen TK, Jørgensen NR. P2X7 receptors are important in cell signalling and pore formation in mature primary human osteblasts. European Calcified Tissue Society meeting, Copenhagen, Denmark, May 2007.

20 Niklas Rye Jørgensen Page Vestergaard P, Jørgensen NR, Mosekilde L, Schwarz P. Effects of strontium ranelate on bone mineral density and fracture risk a meta-analysis. European Calcified Tissue Society meeting, Copenhagen, Denmark, May Nielsen BR, Jørgensen NR, Schwarz P. Primary and secondary prophylaxis to the use of inhaled glucocorticoid in primary health care. European Calcified Tissue Society meeting, Copenhagen, Denmark, May Syberg S, Petersen S, Jørgensen NR. Regional differences in P2X7 receptor regulation of bone metabolism in P2X7 knock-out mice. European Calcified Tissue Society meeting, Copenhagen, Denmark, May Ellegaard M, Stahlhut M, Petersen JS, Pedersen S, Jørgensen NR, Schwarz P. The effect of amylin(1-8) on bone in ovariectomized rats. European Calcified Tissue Society meeting, Copenhagen, Denmark, May Jensen JEB, Hitz M, Schwarz P, Jørgensen NR, Hyldstrup L. Biochemical markers of bone turnover; initial response to PTH(1-34) treatment and relation to changes in BMD and BMC. European Calcified Tissue Society meeting, Copenhagen, Denmark, May Vestergaard P, Jørgensen NR, Schwarz P, Mosekilde L. Effects of treatment with fluoride on bone mineral density and fracture risk a meta-analysis. European Calcified Tissue Society meeting, Copenhagen, Denmark, May Petersen S, Syberg S, Henriksen Z, Schwarz P, Jensen JE, Sørensen OH, Jørgensen NR. The purinergic P2X7 receptor is responsible for ovariectomy-induced bone loss in mice. Prize poster at the research day, Copenhagen University Hospital Glostrup, Glostrup, Denmark. September Henriksen Z, Nissen N, Schwarz P, Larsen TK, Jørgensen NR. P2X7 receptors are important in cell signalling and pore formation in mature primary human osteoblasts. Research day, Copenhagen University Hospital Glostrup, Glostrup, Denmark. September Kringelbach TM, Bonewald L, Jørgensen NR. P2 receptor signalling in osteocyte networks. Research day, Copenhagen University Hospital Glostrup, Glostrup, Denmark. September Syberg S, Petersen S, Teilmann J, Jensen JE, Schwarz P, Jørgensen NR. Ovariectomy-induced bone loss is reduced by the P2X7 agonist BzATP in mice. Research day, Copenhagen University Hospital Glostrup, Glostrup, Denmark. September Johansen ZJ, Syberg S, Schwarz P, Jørgensen NR. The expression of in vitro markers in osteoblasts of different origin. Research day, Copenhagen University Hospital Glostrup, Glostrup, Denmark. September Syberg S, Petersen S, Teilmann J, Jensen JE, Schwarz P, Jørgensen NR. Ovariectomy-induced bone loss is reduced by the P2X7 agonist BzATP in mice. American Society for Bone and Mineral Research, Honolulu, HI, September Petersen S, Syberg S, Henriksen Z, Schwarz P, Jensen JE, Sørensen OH, Jørgensen NR. The purinergic P2X7 receptor is responsible for ovariectomy-induced bone loss in mice. Plenary poster at American Society for Bone and Mineral Research, Honolulu, HI, September Bours MJL, van Helden S, Jørgensen NR, Dagnelie PC. Purinergic signaling in human osteoporosis: design of a cross-sectional genetic epidemiological study to detect diseaserelated aberrations in P2 receptor genes. Purines Copenhagen, June/July Jørgensen NR, Ohlendorff SD, Aarup R, Petersen S, Schwarz P, Tofteng CL, Husted L, Langdahl BL, Wiley J. P2 receptor single nucleotide polymorphisms and bone metabolism. Purines Copenhagen, June/July Kringelbach TM, Schwarz P, Novak I, Bonewald LF, Vang O, Jørgensen NR. P2 receptors are functionally expressed in MLO-Y4 osteocytes. Purines Copenhagen, June/July Jørgensen NR, Husted LB, Schwarz P, Tofteng CL, Jensen JB, Nissen N, Eiken, Ødum L, Langdahl BL. Single Nucleotide Polymorphisms in the P2X7 Receptor Gene are associated with increased Postmenopausal Bone Loss and Fracture Incidence. American Society for Bone and Mineral Research, Montreal, Canada, September 2008.

21 Niklas Rye Jørgensen Page Syberg S, Schwarz P, Jensen JEB, Jørgensen NR. The implications of P2X7 in bone metabolism in mice. The 7 th Baltic Bone conference, Nyborg, Denmark, August Kringelbach TM, Schwarz P, Novak I, Vang O, Bonewald LF, Jørgensen NR. The identification of P2 receptors in human osteocytes. The 7 th Baltic Bone conference, Nyborg, Denmark, August Petersen S, Syberg S, SchwarzP, Henriksen Z, Jørgensen NR. The Purinergic P2X7 Receptor might be involved in the Regulation of Fat Metabolism. American Society for Bone and Mineral Research, Denver, CO, USA, September Henriksen Z, Brixen K, Eiken P, Jensen JEB, Langdahl BL, Schwarz P, Tofteng CL, Jørgensen NR. The Arg312Ser Single Nucleotide Polymorphism in the P2Y2 Receptor Gene is associated with Postmenopausal Rate of Bone Loss.American Society for Bone and Mineral Research, Denver, CO, USA, September Bours MJL, Jørgensen NR, van Helden S, van Rhijn L, Geusens P, Wesselius A, Dagnelie PC. Design of a study on associations between P2 receptor polymorphisms and osteoporosis risk. EULAR, Copenhagen, Denmark, June Hansen RR, Syberg S, Jørgensen NR, Ding M, Nasser A, Heegaard AM. Investigating the role of the purinergic P2X 7 receptor in bone cancer pain. European Calcified Tissue Society meeting, Glasgow, Scotland, May Syber S, Schwarz P, Petersen S, Jensen JEB, Steinberg TH, Gartland A, Teilmann J, Chessell I, Jørgensen NR. Bone status of P2X7 knockout mice in two different strains. Purines 2010 Conference, Tarragona, Spain, May/June Schwarz P, Jørgensen NR, Jensen LT, Vestergaard P. Bone mineral density difference between right and left hip. International Osteoporosis Foundation, Rome, April Dahl M, Jørgensen NR, Pinholt EM. Titanium granule carriers in adipose mesenchymal stem cell osteoblast in vitro mineralization. European Calcified Tissue Society meeting, Athens, Greece, May Syberg S, Petersen S, Orriss I, Schwarz P, Jørgensen NR. ATP treatment potentiates the positive effect of PTH on ovariectomy-induced bone loss in mice. European Calcified Tissue Society meeting, Athens, Greece, May Petersen S, Syberg S, Riis M, Schwarz P, Jørgensen NR. Differences in fat and bone metabolism between genders are partially mediated by the P2X7 receptor. European Calcified Tissue Society meeting, Athens, Greece, May Kvist TM, Aarup RR, Henriksen Z, Brixen K, Eiken P, Jensen JEB, Langdal BL, Tofteng CL, Schwarz P, Jørgensen NR. Association between SNPs in P2Y2 purinergic receptors in postmenopausal women and bone status in vivo. European Calcified Tissue Society meeting, Athens, Greece, May Schwarz P, Jørgensen NR, Mosekilde L, Vestergaard P. The evidence for efficacy of osteoporosis treatment in men with primary osteoporosis. European Calcified Tissue Society meeting, Athens, Greece, May Rasmussen AQ, Jørgensen NR, Schwarz P. Clinical and biochemical beneficial effect in four cases of familial hypocalciuric hypercalcemia treated with cinacalcet for up to three years. European Calcified Tissue Society meeting, Athens, Greece, May Orriss IR, Syberg S, Brandao-Burch A, Hajjawi M, Arnett TR, Jorgensen NR. Clopidogrel (Plavix ), a P2Y 12 receptor antagonist, inhibits osteoblast function in vitro and decreases trabecular bone in vivo. American Society for Bone and Mineral Research, San Diego, CA, USA, September Jørgensen NR, Agca C, Syberg S, Wang N, Petersen S, Gartland A, Schwarz P, Agca Y. Increased expression of the purinergic P2Y2 receptor results in increased resorption and decreased bone in vivo. American Society for Bone and Mineral Research, San Diego, CA, USA, September Petersen S, SybergS, Schwarz P, Jørgensen NR. Effects of adenosine triphosphate (ATP) on bone and fat metabolism. American Society for Bone and Mineral Research, San Diego, CA, USA, September 2011.

22 Niklas Rye Jørgensen Page Durup D, Plovst JR, Jensen LT, Frandsen E, Jørgensen NR, Petersen S, Heegaard AM. Assessement of Bone Associated Pain in the Aged Osteomalacic Rat. American Society for Bone and Mineral Research, San Diego, CA, USA, September Vangsted A, Klausen T, Gang AO, Gimsing P, Abildgaard N, Andersen NF, GregersenH, Nexø BA, Vogel U, Schwarz P, Jørgensen NR. Genetic variation in the P2X7 receptor and the association to risk of disease, effect of high-dose treatment and bone disease in multiple myeloma. International Myeloma Workshop, Paris, May Grove EJ, Wurtz M, Schwarz P, Jørgensen NR, Vestergaard P. Clopidogrel and risk of GI events. European Society of Cardiology, Munich, Germany, August Xu J, Hansen SS, Jørgensen NR, Saurabh G. Role of P2Y2 receptors in neuropathic pain and sex difference. 22 nd Neuropharmacology Conference, New Orleans, USA, October Kvist TM, Petersen S, Frederiksen A, Syberg S, Jørgensen NR, Schwarz P. Inflammatorisk knogletab i en dyremodel - er P2X7 receptoren involveret? Danish Endocrine Society, Aalborg, Denmark, January Jørgensen NR, Vestergaard P,Schwarz P, Iversen HK. The platelet-inhibitor Clopidogrel increases risk of bone fractures in stroke patients. American Society for Bone and Mineral Research, Baltimore, MD, USA, October Petersen S, Syberg S, Schwarz P, Jørgensen NR. P2X7 receptors do they have a role in commitment of mesenchymal stem cells? American Society for Bone and Mineral Research, Baltimore, MD, USA, October Shanbhogue V, Hansen S, Jørgensen NR, Brixen K, Gravholt C. Bone geometry, volumetric density, microarchitecture and estimated bone strength in Klinefelter syndrome. Controversies in bone muscle and joint diseases, Brussels, Belgium, November Rasmussen AQ, Jørgensen NR, Tfelt-Hansen J, Bevilacqua M, Schwarz P. A986S or the R990G polymorphism in CASR does not explain hypercalciuria and low normal serum calcium. European Congress of Endocrinology, Copenhagen, Denmark, April Kvist, Petersen S, Frederiksen AG, Syberg S, Jørgensen NR, Schwarz P. Bone loss in inflammation-mediated osteoporosis a role for the p2x7 receptor? European Congress of Endocrinology, Copenhagen, Denmark, April Frederiksen AG, Lysgaard AL, Iversen E, Jørgensen NR, Schwarz P. BMI might be overestimated in elderly and old osteoporosis patients suffering vertebral fracture(s). European Union Geriatric Medicine Society meeting, Venice, Italy, October Lysgaard AL, Frederiksen AG, Iversen E, Jørgensen NR, Schwarz P. Zoledronic acid does not improve BMD in elderly and old women after 24 months of treatment in an out-patient clinic. European Union Geriatric Medicine Society meeting, Venice, Italy, October Knudsen A, Jørgensen NR, Katzenstein TL, Kronborg G, Obel N, Benfield T, Gerstoft J, Kjær A, Lebech A-M. Plasma plasminogen activator inhibitor-1 predicts first-time myocardial infarction in human immunodeficiency infected individuals. European AIDS Clinical Society, Brussels, Belgium, October Syberg S, Agrawal A, Petersen S, Jensen JEB, Schwarz P, Gartland A, Bös M, Jørgensen NR. The P2X7 antagonist AFC-5261 rescues ovariectomy-induced bone loss in mice. American Society for Bone and Mineral Research, Houston, TX, USA, September Dahl M, Syberg S, Jørgensen NR, Pinholt EM. 3D Scaffolding with Adipose Derived Mesenchymal Stem Cells Their Osteogenicity and Osteoblast Mineralization. American Society for Bone and Mineral Research, Houston, TX, USA, September Ellegaard M, Karlsson M, Lorentzon M, Ohlsson C, Mellström D, Ljunggren Ö, Schwarz P, Jørgensen NR. Association of P2Y 2 Receptor Single-Nucleotide Polymorphism with Bone Mineral Density in Elderly Men. American Society for Bone and Mineral Research, Houston, TX, USA, September Neldam CA, Jørgensen NR, Pinholt EM. Synchrotron µct evaluation of peri-implant hard tissues. American Society for Bone and Mineral Research, Houston, TX, USA, September 2014.

23 Niklas Rye Jørgensen Page Rasmussen AQ, Jørgensen NR, Schwarz P. Identification and functional characterization of a novel activating mutation in the human calcium-sensing receptor gene, responsible for autosomal dominant hypocalcemia. American Society for Bone and Mineral Research, Houston, TX, USA, September Madsbad S, Jørgensen NR, Rabøl R, Chu PL, Stender-Petersen K, Jennum P. The impact of nocturnal hypoglycaemia on sleep in subjects with type 2 diabetes. European Association for the Study of Diabetes Meeting, Vienna, Austria, September Gurzawska K, Svava R, Yihua Y, Haugshøj K, Dirscher K, Nielsen MW, Jørgensen B, Ulvskov P, Berglundh T, Jørgensen NR, Gotfredsen K. Surface nanocoating with pectins of titanium implants in rabbit model. International Association for Dental Research, Madsbad S, Jørgensen NR, Rabøl R, Chu PL, Stender-Petersen K, Jennum P. The impact of nocturnal hypoglycaemia on sleep in subjects with type 2 diabetes. 10 th International Diabetes Federation-Western Pacific Region Congress, Singapore, November Ishøy PL, KnopFK, Broberg BV, Baandrup L, Fagerlund B, Jørgensen NR, Andersen UB, Rostrup E, Glenthøj BY, Ebdrup BH. Treatment of antipsychotic-associated obesity with a GLP-1 receptor agonist: protocol for an investigator-initiated prospective, randomised, placebo-controlled, double-blinded intervention trial - the TAO study. In addition, my group has presented several posters at the scientific meetings at Hvidovre Hospital and Glostrup Hospital every year ( Forskningens Dag ). 7.4 PUBLICATIONS AND PRESENTATIONS IN POPULAR SCIENTIFIC CONNECTION PAPERS IN POPULAR SCIENTIFIC CONNECTION 1. Jørgensen NR, Vadstrup S. Instruction for patients in treatment with Didronate. PraxInfo Jensen JEB, Jørgensen NR. Knoglescanning ved osteoporose, hvor skal man måle og hvilken metode skal anvendes? (Bone scan in osteoporosis where to measure and which method to use. InfoMed 2001;12: Jørgensen NR. Når knoglerne snakker (When bones talk). Apropos Schwarz P, Jørgensen NR. Ung og med øget risiko for knogleskørhed (Young and with increased risk of osteoporosis). Apropos 2004;4: Jørgensen NR. Risk factors for osteoporosis. Best Practice Jørgensen NR, Schwarz P. Et dansk ledet europæisk samarbejde om osteporoseforskning. Apropos PRESENTATIONS IN POPULAR SCIENTIFIC CONNECTION 1. Bone biology and osteoporosis. Invited lecture. Open University (Folkeuniversitetet) series on Osteoporosis, University of Copenhagen, Denmark. November Bone biology and osteoporosis. Invited lecture. Open University (Folkeuniversitetet) series on Osteoporosis, University of Copenhagen, Denmark. November Bone biology and osteoporosis. Invited lecture. Open University (Folkeuniversitetet) series on Osteoporosis, Roskilde University, Denmark. November Bone biology and osteoporosis. Invited lecture. Open University (Folkeuniversitetet) series on Osteoporosis, University of Copenhagen, Denmark. November Bone biology. Invited lecture. Open University (Folkeuniversitetet) series on Osteoporosis, University of Copenhagen, Denmark. Fall Bone biology. Invited lecture. Open University (Folkeuniversitetet) series on Osteoporosis, University of Copenhagen, Denmark. Spring TV interview on new developments in osteoporosis research. TV2 News (6 minutes direct interview). August Radio interview on new developments in osteoporosis research. Radioavisen. August 2011.

24 Niklas Rye Jørgensen Page Osteoporosis at the cellular level how does the treatment work? Invited lecture. Open University (Folkeuniversitetet) series on Osteoporosis, University of Copenhagen, Denmark. February ATPBone a European research collaboration on osteoporosis. Invited lecture. The Danish Osteoporosis Patient Organization (Osteoporoseforeningen), Slagelse, Denmark. Spring ATPBone a European research collaboration on osteoporosis. Invited lecture. The Danish Osteoporosis Patient Organization (Osteoporoseforeningen), Holbæk, Denmark. Spring Radio interview on the risk of fractures when taking clopidogrel. Radio 24/7 direct interview. Spring New knowledge about osteoporosis the results of a joint European project. Invited lecture. The Danish Osteoporosis Patient Organization (Osteoporoseforeningen), Næstved, Denmark. October RESEARCH FUNDING 8.1 FUNDING AWARDS HELD DURING THE LAST FIVE YEARS AS MAIN APPLICANT 1. European Commission s 7 th Framework Programme Kr ,- I was coordinator. Project on the role of P2 receptors in bone metabolism 2. Toyota Fonden. For purchase of equipment (NovoStar) 2010 Kr ,- 3. The Danish Research Council. From Kr ,- Project on mechanotransduction in bone and the role of P2 receptors 4. Novo Nordisk Foundation For purchase of loading equipment Kr ,- AS CO-APPLICANT 5. Copenhagen County Hospital Corporation Kr ,- Main applicant: Peter Schwarz 6. The Danish Research Council Kr ,- Main applicant: Peter Schwarz 7. University of Copenhagen. PhD stipend Kr ,- Main applicant: Tina Marie Kringelbach 8. The Danish Research Council (Det Strategiske Forskningsråd) Kr ,- Main applicant: Iben Damager 9. University of Copenhagen (Puljen for interdiscipl. forskning) Kr ,- Main applicant: Sine Larsen 8.2 PARTICIPATION IN INTERNATIONAL RESEARCH PROGRAMMES 1. Coordinator of the ATPBone consortium which was funded by the European Commission s 7 th Framework Programme Participant in EU COST action aiming at establishing a pan-european Network in Inflammation. Application is submitted to the EU Co-founder and member of steering committee of consortium to apply for EU funding through the Horizon2020 program with the topic of The role of purinergic signaling in complications to type 2 diabetes EXISTING COMMERCIAL COLLABORATIONS

25 Niklas Rye Jørgensen Page Novo Nordisk: A trial to investigate the impact of nocturnal hypoglycaemia on sleep in subjects with type 2 diabetes. Scientific joint project in which I serve as the laboratory coordinator. Funding from the project covers the cost of blood sampling, analysis and coordination. Budget for laboratory part kr , Lundbeck Pharma A/S: Collaborative project on the effects of P2X7 receptor compounds on bone biology. Does not involve direct financial remuneration, but instead access to compounds, technology and knowhow. CONFIDENTIAL INFORMATION 3. Lundbeck Pharma A/S. Collaborative project on a characterization of a newly discovered mutation leading to a severe syndrome involving a bone phenotype of low bone mass. CONFIDENTIAL INFORMATION 4. Lundbeck Pharma A/S: Negotiations on a project addressing the effects of P2X7 compounds on P2X7 receptors with different genetic mutations. Possible involvement in a larger grans application on the subject. CONFIDENTIAL INFORMATION 9. RESEARCH COLLABORATIONS INTERNATIONAL COLLABORATIONS 1. Professor Francesco Di Virgilio, University of Ferrara, Italy on projects investigating the role of the P2X7 receptor in cancer. 2. Professor James Wiley, University of Melbourne, Australia on projects determining the role of P2 receptor polymorphisms in bone health and disease 3. Dr. Alison Gartland, University of Sheffield, UK, on projects relating to P2X7 and P2Y2 knockout mouse models. Also on in vivo studies of different P2 receptor active compounds. 4. Dr. Isabel Orriss, Royal Veterinary College, London, UK on the side effects of platelet inhibitors on bone 5. Professor Yuksel Agca, University of Missouri in Columbia, USA. Projects on P2Y2 overexpressing rats and bone and on studies on the effects of ovary transplant on bone mass and quality. NATIONAL COLLABORATIONS 1. Research Center for Ageing and Osteoporosis (FAO). Close collaboration with Professor Peter Schwarz (University of Copenhagen) and Professor Bo Abrahamsen (OPEN, University of Southern Denmark). My research group is part of the research center, so we have several joint projects on bone/osteporosis. 2. ATPSignal: Resarch network with Professor Ivana Novak, Assoc. Professor Anne-Marie Heegaard and Assoc. Professor Stephan Pless (all University of Copenhagen). Joint projects on P2X7 receptor biology, and with regular scientific meetings with participation of members from all four groups. 3. CoNeXT: Interdisciplinary research group with Professor Sine Larsen as the principal investigator. The collaboration focuses on Fertilizing the ground and harvesting the full potential of the new neutron and X-ray research infrastructures close to Copenhagen University. 4. Assoc. Professor Helle Iversen, Dept. of Neurology, Glostrup Hospital. Collaboration on projects relating to bone health in stroke patients including the clinical effects of clopidogrel on bone in stroke patients. 5. Assoc. Professor Annette Vangsted on projects on the role of the P2X7 receptor in multiple myeloma and in myeloma bone disease. 6. Professor Poul Jennum, Dept. of Neurophysiology, Glostrup Hospital, Professor Sten Madsbad, Dept. of Endocrinology, Hvidovre Hospital, and Novo Nordisk on the impact of nocturnal hypoglycaemia on sleep and hormones in type 2 diabetes patients.

26 Niklas Rye Jørgensen Page Assoc. Professor Eva Wulff Helge, University of Copenhagen on several projects addressing physical exercise and bone turnover markers. 8. Assoc. Professor Henrik Vestergaard, University of Copenhagen on a project on changes in bone turnover after bariatric surgery. 9. Assoc. Professor Pia Eiken, Hillerød Hospital, on project on bone metabolism in patients with type 2 diabetes. 10. Professor Ming Ding, SDU, on several projects addressing the role of P2 receptor signaling and bone structure in vivo. 11. Dr. Stinus Hansen, Esbjerg Hospital, on a project examining the effect of gastric bypass on bone structure (HR-pQCT) and bone turnover markers. 12. Professor Peter Vestergaard, Aalborg University, on project addressing the role of clopidogrel and other platelet inhibitors in fracture risk (epidemiological studies). 13. Assoc. Professor Ole Rintek Madsen, Gentofte Hospital on project on bone turnover markers in rheumatological patients treated with biological anti-rheumatic agents. 14. Dr. Trine Jensen David, Hvidovre Hospital and Professor Mikkel Østergaard, VRR, Glostrup Hospital on a project on bone metabolism in rheumatoid arthritis patients in DMARD therapy. 15. Professor Allan Linneberg, Glostrup Hospital on project on reference ranges of bone turnover markers in healthy individuals as well as other studies in the Glostrup cohorts. 16. Professor Merete Hetland on the Danish Rheuma Biobank. 17. Lundbeck Pharma A/S: Collaborative project on the effects of P2X7 receptor compounds on bone biology. 18. Lundbeck Pharma A/S. Collaborative project on a characterization of a newly discovered mutation leading to a severe syndrome involving a bone phenotype of low bone mass.